Rw2G023760
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
N/A
Physical Location & Seq
Forward (+)
34819317 .. 34823730
4414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023760.1

Sequence Viewer

Length: 1806 bp
ATGGACAACGTAGATTGGAATCTTGAGCTCCAGCGCCTGAAGAGAAGCAAGCGCCGCCAAGTAAGGAACTACCCGGCTCGGAAAACAAGTTTCTTTGTTGAATTCCGGCACGGCCTTCCCGGCGCCGGCCACCGTGAAGACTTTCTTACTGCCGGACGTGCTTTTCAAGAACTGAGGGATATGCCTCCACACCCCAATGTGGTTAGAGTGATTGAGTGGGTCGACAACGACGTCCAAGGCGGTCCTGGTTTTCATGTCGTTCTTGAAAGCTCTCAAATGACTTTGAGAAGTTTACTACAAAAATGTGGACGGATTCCTGAGGAGAAAGCCCAGTTTATTATGCTCCAACTCTTGAAAGGAGTTGAGCACTGTCATGAGAATCAACTCCTGCATGGAGATATTAGACCCGAAAATTTAATAATTGATGAAGGCAGTTTTCAGATCAAACTGACTGGGTTTGATATTGCTCGTCCCCTATGGTCACTTCCCGTCGATCCCCTGCATAATCTAAAATCTCTTTCTGGCTGTCTAAGCTACCAGAGTCCAGAGCTTCTGTTGAATGACATCCGAAACAGTCCTGCAGTGGACATCTGGTCCTGCGCTTGTGTCTTTGCTGAAATGCTCACCGGACAGCTTCTTTTTCAAGGGGACAACTCTGAGTCAGCTACTGGACAACTAAAGTCAATCATTTCTCTATTTGCAACTCCTAGTGAGCCGGAAGTGAGTGATTTACTTAAGTCCTTCCATCAGGGTTCACTTGATAACATGAGTGATCGGCTTATATCATACCTCTTCCATCGTTATGCACCTATCGACTTGACTGATGCTATGCCTGATGTTTTGGAGTGCTTGCGGCCTTGGGGACTTGACCTGCTCTCTAGGATGTTAAATGTGAATCCAACTGAAAGAATTCCATTGGAATTGTGCCTACATCATCCATACTTCTTAATGAATCCAGAGGACAAGGCGGAATTGGATGACACCGTACCTATCCGATTAATTAGGAATTATAGCGTGAGATTGGCTTATAAATTTCGATTTATGGAGAAGGATCGTATTAGGATTCTTAATGCTACTCGTGGACTGTCGTGTCTTCATGCTCTTCCTCCACATCCGAACATAGTATCATTAGAGGAGTGGAATGATTGGGATGTCAGCGGTCATGAGAGGGGTTTTCACGTGGTTCTAAGGTATCAATCAACCAAAAGTTTGAAAAGTTTCATCCGCTGCAGATCAGATCTTAGAACTATATGTTCGATATTGTACCAACTCTTAATAGGAGTGGCACATTGCCACTCCCATGGAGTTTTACATGGAGACATTCGACCAGAAAACCTAGTGGTAGATCATAGGACTGGAGTTCTACAGATCAGTGAATTTGACATTGCTCGCCGTTTAGGGGTGCCATTGGATCCAGATATCCGTTTTCCAGCCGGTTTTCTATCTTACCAATCCCCTGAGCTGCTTCTCGGTGACACCACTAATAATGCTGCATCTGATATATGGTCTGTTGGCTGTATCTTTGCTGAGCTGCTGTTGGGAAAAGTCATGTTCACTGGGAATTCTGTGTTTCAGCAGATGGTTTCTATTTTCAGATTTCTTGGAACTCCCCCAGTGGAGGACTTGGGGGACTTGCGTCCACCTAGCTTGTACAGACAATATCCACCACAGAATTTGAGCAATATTTTCCCACAAGAGACCGTTCGTCTCATTTCGGGAATGTTGACCATAAACCCTGCCAACAGGTTTTCTGTGCAGGACTGCCTCGATGATCCACTTTTAGCAGATGAGATAAACAGGCATTAG

Protein Analysis

601

Amino Acids

68.45

Weight (kDa)

5.94

Isoelectric Point (pI)

46.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 56 - 231 5.7e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 58 - 315 2.6e-38 Protein kinase domain
Pkinase PF00069 356 - 593 2.1e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 358 - 518 3.2e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1029
AasI GACNNNNNNGTC 2 cut(s) 230, 1089
AatII GACGTC 1 cut(s) 234
Acc36I ACCTGC 1 cut(s) 879
AccB1I GGYRCC 2 cut(s) 122, 1402
AccI GTMKAC 1 cut(s) 222
AciI CCGC 6 cut(s) 55, 240, 853, 968, 1158, 1225
AclWI GGATC 5 cut(s) 488, 1059, 1406, 1419, 1766
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 7 cut(s) 101, 412, 909, 1031, 1376, 1561, 1672
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 1180
AcyI GRCGYC 2 cut(s) 123, 231
AfaI GTAC 3 cut(s) 987, 1265, 1652
AfiI CCNNNNNNNGG 4 cut(s) 125, 199, 1399, 1618
AflII CTTAAG 1 cut(s) 734
AgsI TTSAA 7 cut(s) 101, 167, 266, 355, 559, 644, 1213
AhdI GACNNNNNGTC 2 cut(s) 592, 1704
AjiI CACGTC 1 cut(s) 158
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 9 cut(s) 28, 270, 534, 550, 634, 665, 1462, 1531, 1647
AluI AGCT 9 cut(s) 28, 270, 534, 550, 634, 665, 1462, 1531, 1647
Alw21I GWGCWC 2 cut(s) 30, 369
Alw26I GTCTC 3 cut(s) 1311, 1691, 1712
AlwI GGATC 5 cut(s) 488, 1059, 1406, 1419, 1766
AlwNI CAGNNNCTG 2 cut(s) 37, 668
AoxI GGCC 3 cut(s) 112, 127, 854
ApeKI GCWGC 4 cut(s) 1227, 1462, 1490, 1531
ApoI RAATTY 7 cut(s) 101, 412, 909, 1031, 1376, 1561, 1672
AseI ATTAAT 1 cut(s) 998
Asp700I GAANNNNTTC 3 cut(s) 141, 909, 1217
AspLEI GCGC 4 cut(s) 36, 54, 125, 602
AspS9I GGNCC 2 cut(s) 242, 594
AsuC2I CCSGG 2 cut(s) 74, 120
AsuHPI GGTGA 2 cut(s) 616, 1484
AvaII GGWCC 2 cut(s) 242, 594
AxyI CCTNAGG 1 cut(s) 318
BamHI GGATCC 1 cut(s) 1411
BanI GGYRCC 2 cut(s) 122, 1402
BanII GRGCYC 1 cut(s) 30
BauI CACGAG 1 cut(s) 1077
BbrPI CACGTG 1 cut(s) 1180
BbsI GAAGAC 2 cut(s) 144, 1085
Bbv12I GWGCWC 2 cut(s) 30, 369
BbvI GCAGC 4 cut(s) 1214, 1449, 1477, 1518
BccI CCATC 3 cut(s) 753, 804, 1573
BceAI ACGGC 2 cut(s) 127, 1377
BciT130I CCWGG 1 cut(s) 246
BcnI CCSGG 2 cut(s) 74, 120
BcoDI GTCTC 3 cut(s) 1311, 1691, 1712
BfaI CTAG 4 cut(s) 708, 879, 1337, 1644
BfmI CTRYAG 3 cut(s) 579, 1228, 1364
BfoI RGCGCY 3 cut(s) 37, 55, 126
BfrI CTTAAG 1 cut(s) 734
BfuAI ACCTGC 1 cut(s) 879
BglI GCCNNNNNGGC 1 cut(s) 120
BglII AGATCT 1 cut(s) 1237
BisI GCNGC 6 cut(s) 55, 854, 1228, 1463, 1491, 1532
BlpI GCTNAGC 1 cut(s) 1527
BlsI GCNGC 6 cut(s) 56, 855, 1229, 1464, 1492, 1533
Bme1390I CCNGG 3 cut(s) 74, 120, 246
Bme18I GGWCC 2 cut(s) 242, 594
BmeRI GACNNNNNGTC 2 cut(s) 592, 1704
BmgBI CACGTC 1 cut(s) 158
BmgT120I GGNCC 2 cut(s) 242, 594
BmiI GGNNCC 3 cut(s) 124, 1404, 1413
BmrFI CCNGG 3 cut(s) 74, 120, 246
BmrI ACTGGG 4 cut(s) 325, 462, 1566, 1607
BmsI GCATC 2 cut(s) 814, 1502
BmuI ACTGGG 4 cut(s) 325, 462, 1566, 1607
BoxI GACNNNNGTC 1 cut(s) 1635
BpiI GAAGAC 2 cut(s) 144, 1085
BpmI CTGGAG 2 cut(s) 14, 1377
Bpu10I CCTNAGC 1 cut(s) 1458
Bpu1102I GCTNAGC 1 cut(s) 1527
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 74, 120
BsaAI YACGTR 1 cut(s) 1180
BsaBI GATNNNNATC 1 cut(s) 18
BsaHI GRCGYC 2 cut(s) 123, 231
BsaI GGTCTC 1 cut(s) 1691
BsaJI CCNNGG 3 cut(s) 235, 857, 1300
BsaWI WCCGGW 1 cut(s) 626
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 199, 1399, 1618
Bse118I RCCGGY 2 cut(s) 125, 1433
Bse1I ACTGG 6 cut(s) 331, 457, 673, 1360, 1561, 1613
Bse21I CCTNAGG 1 cut(s) 318
Bse3DI GCAATG 2 cut(s) 1288, 1383
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 235, 857, 1300
BseGI GGATG 7 cut(s) 564, 888, 934, 982, 1111, 1156, 1221
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 4 cut(s) 125, 199, 1399, 1618
BseMI GCAATG 2 cut(s) 1288, 1383
BseMII CTCAG 5 cut(s) 164, 309, 648, 1449, 1518
BseNI ACTGG 6 cut(s) 331, 457, 673, 1360, 1561, 1613
BseRI GAGGAG 2 cut(s) 335, 1148
BseXI GCAGC 4 cut(s) 1214, 1449, 1477, 1518
BsgI GTGCAG 1 cut(s) 1775
BshFI GGCC 3 cut(s) 114, 129, 856
BshNI GGYRCC 2 cut(s) 122, 1402
BsiHKAI GWGCWC 2 cut(s) 30, 369
BsiSI CCGG 8 cut(s) 74, 106, 120, 126, 153, 627, 716, 1434
BslFI GGGAC 4 cut(s) 456, 662, 876, 1643
BslI CCNNNNNNNGG 4 cut(s) 125, 199, 1399, 1618
BsmAI GTCTC 3 cut(s) 1311, 1691, 1712
BsmBI CGTCTC 1 cut(s) 1712
BsmFI GGGAC 4 cut(s) 456, 662, 876, 1643
BsnI GGCC 3 cut(s) 114, 129, 856
Bso31I GGTCTC 1 cut(s) 1691
Bsp1286I GDGCHC 2 cut(s) 30, 369
Bsp1407I TGTACA 1 cut(s) 1650
Bsp1720I GCTNAGC 1 cut(s) 1527
Bsp19I CCATGG 1 cut(s) 1300
BspACI CCGC 6 cut(s) 55, 240, 853, 968, 1158, 1225
BspANI GGCC 3 cut(s) 114, 129, 856
BspCNI CTCAG 5 cut(s) 165, 310, 649, 1450, 1519
BspHI TCATGA 2 cut(s) 373, 1162
BspLI GGNNCC 3 cut(s) 124, 1404, 1413
BspMAI CTGCAG 2 cut(s) 583, 1232
BspMI ACCTGC 1 cut(s) 879
BspPI GGATC 5 cut(s) 488, 1059, 1406, 1419, 1766
BspQI GCTCTTC 1 cut(s) 1107
BspT107I GGYRCC 2 cut(s) 122, 1402
BspTI CTTAAG 1 cut(s) 734
BspTNI GGTCTC 1 cut(s) 1691
BsrDI GCAATG 2 cut(s) 1288, 1383
BsrFI RCCGGY 2 cut(s) 125, 1433
BsrGI TGTACA 1 cut(s) 1650
BsrI ACTGG 6 cut(s) 331, 457, 673, 1360, 1561, 1613
BssAI RCCGGY 2 cut(s) 125, 1433
BssECI CCNNGG 3 cut(s) 235, 857, 1300
BssNI GRCGYC 2 cut(s) 123, 231
BssSI CACGAG 1 cut(s) 1077
BssT1I CCWWGG 3 cut(s) 235, 857, 1300
Bst2BI CACGAG 1 cut(s) 1077
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 6 cut(s) 134, 371, 575, 985, 1086, 1702
Bst6I CTCTTC 3 cut(s) 35, 797, 1107
BstACI GRCGYC 2 cut(s) 123, 231
BstAFI CTTAAG 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 1650
BstBAI YACGTR 1 cut(s) 1180
BstC8I GCNNGC 4 cut(s) 50, 127, 851, 1390
BstDEI CTNAG 8 cut(s) 173, 318, 530, 657, 1187, 1241, 1458, 1527
BstDSI CCRYGG 1 cut(s) 1300
BstF5I GGATG 7 cut(s) 564, 888, 934, 982, 1111, 1156, 1221
BstH2I RGCGCY 3 cut(s) 37, 55, 126
BstHHI GCGC 4 cut(s) 36, 54, 125, 602
BstMAI GTCTC 3 cut(s) 1311, 1691, 1712
BstMWI GCNNNNNNNGC 4 cut(s) 54, 120, 158, 531
BstNI CCWGG 1 cut(s) 246
BstPAI GACNNNNGTC 1 cut(s) 1635
BstSCI CCNGG 3 cut(s) 72, 118, 244
BstSFI CTRYAG 3 cut(s) 579, 1228, 1364
BstV1I GCAGC 4 cut(s) 1214, 1449, 1477, 1518
BstV2I GAAGAC 2 cut(s) 144, 1085
BstX2I RGATCY 2 cut(s) 1237, 1411
BstXI CCANNNNNNTGG 1 cut(s) 1301
BstYI RGATCY 2 cut(s) 1237, 1411
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 114, 129, 856
BtgI CCRYGG 1 cut(s) 1300
BtrI CACGTC 1 cut(s) 158
BtsCI GGATG 7 cut(s) 564, 888, 934, 982, 1111, 1156, 1221
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 5 cut(s) 367, 588, 1378, 1554, 1620
BveI ACCTGC 1 cut(s) 879
Cac8I GCNNGC 4 cut(s) 50, 127, 851, 1390
CaiI CAGNNNCTG 2 cut(s) 37, 668
CciI TCATGA 2 cut(s) 373, 1162
CfoI GCGC 4 cut(s) 36, 54, 125, 602
Cfr10I RCCGGY 2 cut(s) 125, 1433
Cfr13I GGNCC 2 cut(s) 242, 594
CseI GACGC 1 cut(s) 1625
Csp6I GTAC 3 cut(s) 986, 1264, 1651
CspCI CAANNNNNGTGG 4 cut(s) 1629, 1656, 1664, 1691
CviAII CATG 9 cut(s) 254, 374, 392, 766, 1097, 1163, 1301, 1313, 1549
CviQI GTAC 3 cut(s) 986, 1264, 1651
DdeI CTNAG 8 cut(s) 173, 318, 530, 657, 1187, 1241, 1458, 1527
DinI GGCGCC 1 cut(s) 124
DrdI GACNNNNNNGTC 2 cut(s) 230, 1089
DriI GACNNNNNGTC 2 cut(s) 592, 1704
DseDI GACNNNNNNGTC 2 cut(s) 230, 1089
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 3 cut(s) 35, 797, 1107
Eam1105I GACNNNNNGTC 2 cut(s) 592, 1704
EarI CTCTTC 3 cut(s) 35, 797, 1107
EciI GGCGGA 1 cut(s) 983
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 235, 857, 1300
Eco24I GRGCYC 1 cut(s) 30
Eco31I GGTCTC 1 cut(s) 1691
Eco32I GATATC 1 cut(s) 1420
Eco47I GGWCC 2 cut(s) 242, 594
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 1180
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 3 cut(s) 101, 909, 1561
EcoRII CCWGG 1 cut(s) 244
EcoRV GATATC 1 cut(s) 1420
EcoT14I CCWWGG 3 cut(s) 235, 857, 1300
EcoT38I GRGCYC 1 cut(s) 30
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 3 cut(s) 235, 857, 1300
Esp3I CGTCTC 1 cut(s) 1712
FaeI CATG 9 cut(s) 257, 377, 395, 769, 1100, 1166, 1304, 1316, 1552
FalI AAGNNNNNCTT 2 cut(s) 129, 161
FaqI GGGAC 4 cut(s) 456, 662, 876, 1643
FatI CATG 9 cut(s) 253, 373, 391, 765, 1096, 1162, 1300, 1312, 1548
FblI GTMKAC 1 cut(s) 222
Fnu4HI GCNGC 6 cut(s) 55, 854, 1228, 1463, 1491, 1532
FokI GGATG 7 cut(s) 551, 895, 921, 989, 1098, 1163, 1208
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 6 cut(s) 55, 854, 1228, 1463, 1491, 1532
FspBI CTAG 4 cut(s) 708, 879, 1337, 1644
GlaI GCGC 4 cut(s) 35, 53, 124, 601
GluI GCNGC 6 cut(s) 55, 854, 1228, 1463, 1491, 1532
GsuI CTGGAG 2 cut(s) 14, 1377
HaeII RGCGCY 3 cut(s) 37, 55, 126
HaeIII GGCC 3 cut(s) 114, 129, 856
HapII CCGG 8 cut(s) 74, 106, 120, 126, 153, 627, 716, 1434
HgaI GACGC 1 cut(s) 1625
HhaI GCGC 4 cut(s) 36, 54, 125, 602
Hin1I GRCGYC 2 cut(s) 123, 231
Hin1II CATG 9 cut(s) 257, 377, 395, 769, 1100, 1166, 1304, 1316, 1552
Hin6I GCGC 4 cut(s) 34, 52, 123, 600
HinP1I GCGC 4 cut(s) 34, 52, 123, 600
HincII GTYRAC 2 cut(s) 223, 1725
HindII GTYRAC 2 cut(s) 223, 1725
HinfI GANTC 8 cut(s) 19, 313, 379, 541, 659, 895, 952, 1063
HpaII CCGG 8 cut(s) 74, 106, 120, 126, 153, 627, 716, 1434
HphI GGTGA 2 cut(s) 616, 1484
Hpy166II GTNNAC 9 cut(s) 223, 293, 308, 586, 755, 1082, 1554, 1640, 1725
Hpy188I TCNGA 9 cut(s) 81, 441, 569, 658, 995, 1116, 1237, 1498, 1595
Hpy8I GTNNAC 9 cut(s) 223, 293, 308, 586, 755, 1082, 1554, 1640, 1725
Hpy99I CGWCG 2 cut(s) 233, 494
HpyAV CCTTC 4 cut(s) 125, 422, 751, 1042
HpyCH4III ACNGT 6 cut(s) 134, 371, 575, 985, 1086, 1702
HpyCH4IV ACGT 4 cut(s) 9, 157, 231, 1179
HpyCH4V TGCA 8 cut(s) 391, 502, 581, 701, 806, 1230, 1493, 1756
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 120, 158, 531
HpyF3I CTNAG 8 cut(s) 173, 318, 530, 657, 1187, 1241, 1458, 1527
HpySE526I ACGT 4 cut(s) 9, 157, 231, 1179
Hsp92I GRCGYC 2 cut(s) 123, 231
Hsp92II CATG 9 cut(s) 257, 377, 395, 769, 1100, 1166, 1304, 1316, 1552
HspAI GCGC 4 cut(s) 34, 52, 123, 600
KasI GGCGCC 1 cut(s) 122
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
LguI GCTCTTC 1 cut(s) 1107
LmnI GCTCC 2 cut(s) 33, 348
Lsp1109I GCAGC 4 cut(s) 1214, 1449, 1477, 1518
LweI GCATC 2 cut(s) 814, 1502
MaeI CTAG 4 cut(s) 708, 879, 1337, 1644
MaeII ACGT 4 cut(s) 9, 157, 231, 1179
MaeIII GTNAC 2 cut(s) 480, 1472
MboII GAAGA 5 cut(s) 52, 149, 784, 1085, 1094
MflI RGATCY 2 cut(s) 1237, 1411
MhlI GDGCHC 2 cut(s) 30, 369
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 550, 668
MmeI TCCRAC 2 cut(s) 370, 923
MroNI GCCGGC 1 cut(s) 125
MroXI GAANNNNTTC 3 cut(s) 141, 909, 1217
MseI TTAA 7 cut(s) 416, 735, 887, 947, 998, 1068, 1274
MslI CAYNNNNRTG 4 cut(s) 195, 372, 801, 1299
MspA1I CMGCKG 2 cut(s) 1158, 1227
MspCI CTTAAG 1 cut(s) 734
MspI CCGG 8 cut(s) 74, 106, 120, 126, 153, 627, 716, 1434
MspR9I CCNGG 3 cut(s) 74, 120, 246
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 4 cut(s) 54, 120, 158, 531
NaeI GCCGGC 1 cut(s) 127
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 2 cut(s) 74, 120
NcoI CCATGG 1 cut(s) 1300
NgoMIV GCCGGC 1 cut(s) 125
NlaIII CATG 9 cut(s) 257, 377, 395, 769, 1100, 1166, 1304, 1316, 1552
NlaIV GGNNCC 3 cut(s) 124, 1404, 1413
NmuCI GTSAC 2 cut(s) 480, 1472
PagI TCATGA 2 cut(s) 373, 1162
PciSI GCTCTTC 1 cut(s) 1107
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PdiI GCCGGC 1 cut(s) 127
PdmI GAANNNNTTC 3 cut(s) 141, 909, 1217
PfeI GAWTC 6 cut(s) 19, 313, 379, 895, 952, 1063
PkrI GCNGC 6 cut(s) 56, 855, 1229, 1464, 1492, 1533
PleI GAGTC 2 cut(s) 549, 667
PluTI GGCGCC 1 cut(s) 126
PmaCI CACGTG 1 cut(s) 1180
PmlI CACGTG 1 cut(s) 1180
PpsI GAGTC 2 cut(s) 549, 667
Ppu21I YACGTR 1 cut(s) 1180
PshAI GACNNNNGTC 1 cut(s) 1635
PshBI ATTAAT 1 cut(s) 998
PsiI TTATAA 1 cut(s) 1029
Psp124BI GAGCTC 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 244
PspCI CACGTG 1 cut(s) 1180
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 124, 1404, 1413
PspPI GGNCC 2 cut(s) 242, 594
PstI CTGCAG 2 cut(s) 583, 1232
PstNI CAGNNNCTG 2 cut(s) 37, 668
PsuI RGATCY 2 cut(s) 1237, 1411
RsaI GTAC 3 cut(s) 987, 1265, 1652
RsaNI GTAC 3 cut(s) 986, 1264, 1651
RseI CAYNNNNRTG 4 cut(s) 195, 372, 801, 1299
SacI GAGCTC 1 cut(s) 30
SalI GTCGAC 1 cut(s) 221
SapI GCTCTTC 1 cut(s) 1107
SaqAI TTAA 7 cut(s) 416, 735, 887, 947, 998, 1068, 1274
SatI GCNGC 6 cut(s) 55, 854, 1228, 1463, 1491, 1532
Sau96I GGNCC 2 cut(s) 242, 594
SchI GAGTC 2 cut(s) 550, 668
ScrFI CCNGG 3 cut(s) 74, 120, 246
SduI GDGCHC 2 cut(s) 30, 369
SfaNI GCATC 2 cut(s) 814, 1502
SfcI CTRYAG 3 cut(s) 579, 1228, 1364
SfoI GGCGCC 1 cut(s) 124
SinI GGWCC 2 cut(s) 242, 594
SmiMI CAYNNNNRTG 4 cut(s) 195, 372, 801, 1299
SmlI CTYRAG 2 cut(s) 23, 734
SmoI CTYRAG 2 cut(s) 23, 734
SsiI CCGC 6 cut(s) 55, 240, 853, 968, 1158, 1225
SspDI GGCGCC 1 cut(s) 122
SspI AATATT 1 cut(s) 1684
SspMI CTAG 4 cut(s) 708, 879, 1337, 1644
SstI GAGCTC 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 72, 118, 244
StyI CCWWGG 3 cut(s) 235, 857, 1300
TaaI ACNGT 6 cut(s) 134, 371, 575, 985, 1086, 1702
TaiI ACGT 4 cut(s) 12, 160, 234, 1182
TaqI TCGA 7 cut(s) 222, 492, 813, 1036, 1256, 1324, 1767
TatI WGTACW 1 cut(s) 1650
TauI GCSGC 2 cut(s) 57, 856
TfiI GAWTC 6 cut(s) 19, 313, 379, 895, 952, 1063
Tru1I TTAA 7 cut(s) 416, 735, 887, 947, 998, 1068, 1274
Tru9I TTAA 7 cut(s) 416, 735, 887, 947, 998, 1068, 1274
TscAI CASTG 5 cut(s) 374, 588, 1378, 1561, 1620
TseFI GTSAC 2 cut(s) 480, 1472
TseI GCWGC 4 cut(s) 1227, 1462, 1490, 1531
Tsp45I GTSAC 2 cut(s) 480, 1472
TspDTI ATGAA 5 cut(s) 242, 441, 965, 1085, 1210
TspGWI ACGGA 2 cut(s) 325, 1412
TspRI CASTG 5 cut(s) 374, 588, 1378, 1561, 1620
Vha464I CTTAAG 1 cut(s) 734
VpaK11BI GGWCC 2 cut(s) 242, 594
VspI ATTAAT 1 cut(s) 998
XapI RAATTY 7 cut(s) 101, 412, 909, 1031, 1376, 1561, 1672
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmiI GTMKAC 1 cut(s) 222
XmnI GAANNNNTTC 3 cut(s) 141, 909, 1217
XspI CTAG 4 cut(s) 708, 879, 1337, 1644
ZraI GACGTC 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.