Rw5G048140

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
N/A
Physical Location & Seq
Reverse (-)
83558952 .. 83559710
759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048140.1

Sequence Viewer

Length: 759 bp
ATGTCGAATGGCAGTTTTGAAGCATTTAAAGCAACAAGACAAGCCATGGATAAGCTCACGAAGGCAGTAGAAAATGATGAGGTGGCTGCCATTGGGGTCTATGGAATGGGAGGAGTTGGGAAGACAACTATGGTCAAACACGTCAGTGCACAAGCCCAAAAAAGGGGACTTTTTGATCATGTGATTATGGTTGTTGTATCCCAAACCCCAAATAGGAGAAATATTCAAGGCGCACTTGAGGATCTGCTGGGCTTAAAATTGGAGGAGGAGTCAGAAGGTGGACGAGCAGATAGATTGCAGAAGGAGACACTTAGAAGAAATAAGATCCTTATAATCTTAAGACGACATTTGGAGACTGGACTTGTCAAGCATTGGAATTCCCAGCTACAACGACATTCAAAAATGCGATTCCAAAGTGCTACTCACCACAAGGAGATTGAATGTTTGCCATACCATGGAGAGCCAAGAAATCATTCATCTAGTATCTTATTAGAAGAAGATGCTTGGAACTTGTTTTTGAAGAAGGCAAGAAAGTCTTTTCAAAAATCTACCGAATTTTCAAGAAAGTCTTTTCAAAAATCTACCGAATTTTGTCAGGTAGCCAAAGAATGTGCTGTTCTCCCAGTTGCATTGATTACAGTTGCGAGGGCACTTGGAGATAAAGATTTTGAAGACTGGAAAGAAGCAGCTCGACGGCTTGAGATGTCTCAACCTGCCAACCTTAACGAGGAGAAAGATGTGTTCAACTGTATAAAGTAG

Protein Analysis

252

Amino Acids

28.69

Weight (kDa)

9.31

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 14 - 177 5.8e-19 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 332
Acc36I ACCTGC 1 cut(s) 721
AccB7I CCANNNNNTGG 1 cut(s) 455
AclWI GGATC 2 cut(s) 249, 319
AcsI RAATTY 3 cut(s) 376, 554, 587
AfiI CCNNNNNNNGG 5 cut(s) 162, 163, 213, 455, 727
AflII CTTAAG 1 cut(s) 337
AflIII ACRYGT 1 cut(s) 139
AjiI CACGTC 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 457, 489
AleI CACNNNNGTG 1 cut(s) 144
AluBI AGCT 3 cut(s) 55, 385, 689
AluI AGCT 3 cut(s) 55, 385, 689
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 3 cut(s) 299, 347, 711
Alw44I GTGCAC 1 cut(s) 147
AlwI GGATC 2 cut(s) 249, 319
ApaLI GTGCAC 1 cut(s) 147
ApeKI GCWGC 2 cut(s) 86, 686
ApoI RAATTY 3 cut(s) 376, 554, 587
Asp700I GAANNNNTTC 1 cut(s) 472
AspLEI GCGC 1 cut(s) 233
AsuHPI GGTGA 1 cut(s) 416
BaeGI GKGCMC 2 cut(s) 151, 652
BbsI GAAGAC 2 cut(s) 128, 678
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 2 cut(s) 73, 698
BceAI ACGGC 1 cut(s) 710
BciVI GTATCC 1 cut(s) 208
BclI TGATCA 1 cut(s) 175
BcoDI GTCTC 3 cut(s) 299, 347, 711
BfaI CTAG 1 cut(s) 480
BfrI CTTAAG 1 cut(s) 337
BfuAI ACCTGC 1 cut(s) 721
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 2 cut(s) 87, 687
BlsI GCNGC 2 cut(s) 88, 688
BmgBI CACGTC 1 cut(s) 142
BmrI ACTGGG 1 cut(s) 617
BmsI GCATC 1 cut(s) 490
BmuI ACTGGG 1 cut(s) 617
BpiI GAAGAC 2 cut(s) 128, 678
BpuEI CTTGAG 2 cut(s) 257, 719
BsaJI CCNNGG 2 cut(s) 45, 454
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 163, 213, 455, 727
Bse1I ACTGG 3 cut(s) 361, 623, 680
BseDI CCNNGG 2 cut(s) 45, 454
BseLI CCNNNNNNNGG 5 cut(s) 162, 163, 213, 455, 727
BseNI ACTGG 3 cut(s) 361, 623, 680
BseRI GAGGAG 4 cut(s) 126, 278, 281, 743
BseSI GKGCMC 2 cut(s) 151, 652
BseXI GCAGC 2 cut(s) 73, 698
BseYI CCCAGC 2 cut(s) 247, 381
BsiHKAI GWGCWC 1 cut(s) 151
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 5 cut(s) 162, 163, 213, 455, 727
BsmAI GTCTC 3 cut(s) 299, 347, 711
BsmFI GGGAC 1 cut(s) 180
Bsp1286I GDGCHC 2 cut(s) 151, 652
Bsp143I GATC 3 cut(s) 175, 241, 324
Bsp19I CCATGG 2 cut(s) 45, 454
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 2 cut(s) 249, 319
BspTI CTTAAG 1 cut(s) 337
BsrI ACTGG 3 cut(s) 361, 623, 680
BssECI CCNNGG 2 cut(s) 45, 454
BssMI GATC 3 cut(s) 175, 241, 324
BssT1I CCWWGG 2 cut(s) 45, 454
Bst4CI ACNGT 2 cut(s) 640, 749
BstAFI CTTAAG 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 311
BstDSI CCRYGG 2 cut(s) 45, 454
BstENI CCTNNNNNAGG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 3 cut(s) 178, 244, 327
BstMAI GTCTC 3 cut(s) 299, 347, 711
BstMBI GATC 3 cut(s) 175, 241, 324
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstSLI GKGCMC 2 cut(s) 151, 652
BstV1I GCAGC 2 cut(s) 73, 698
BstV2I GAAGAC 2 cut(s) 128, 678
BstX2I RGATCY 2 cut(s) 241, 324
BstYI RGATCY 2 cut(s) 241, 324
BsuI GTATCC 1 cut(s) 208
BtgI CCRYGG 2 cut(s) 45, 454
BtrI CACGTC 1 cut(s) 142
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 1 cut(s) 721
CfoI GCGC 1 cut(s) 233
CviAII CATG 3 cut(s) 46, 179, 455
DdeI CTNAG 1 cut(s) 311
DpnI GATC 3 cut(s) 177, 243, 326
DpnII GATC 3 cut(s) 175, 241, 324
DraI TTTAAA 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 45, 454
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoRI GAATTC 1 cut(s) 376
EcoT14I CCWWGG 2 cut(s) 45, 454
ErhI CCWWGG 2 cut(s) 45, 454
FaeI CATG 3 cut(s) 49, 182, 458
FaiI YATR 9 cut(s) 47, 102, 131, 180, 188, 332, 451, 456, 752
FalI AAGNNNNNCTT 6 cut(s) 219, 251, 520, 552, 553, 585
FaqI GGGAC 1 cut(s) 180
FatI CATG 3 cut(s) 45, 178, 454
FbaI TGATCA 1 cut(s) 175
Fnu4HI GCNGC 2 cut(s) 87, 687
Fsp4HI GCNGC 2 cut(s) 87, 687
FspBI CTAG 1 cut(s) 480
GlaI GCGC 1 cut(s) 232
GluI GCNGC 2 cut(s) 87, 687
GsaI CCCAGC 2 cut(s) 251, 385
HhaI GCGC 1 cut(s) 233
Hin1II CATG 3 cut(s) 49, 182, 458
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HinfI GANTC 2 cut(s) 269, 408
HphI GGTGA 1 cut(s) 416
Hpy166II GTNNAC 2 cut(s) 149, 281
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 2 cut(s) 58, 561
Hpy8I GTNNAC 2 cut(s) 149, 281
Hpy99I CGWCG 1 cut(s) 696
HpyAV CCTTC 4 cut(s) 55, 269, 295, 517
HpyCH4III ACNGT 2 cut(s) 640, 749
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 3 cut(s) 149, 298, 629
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 311
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 3 cut(s) 49, 182, 458
HspAI GCGC 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 175
Kzo9I GATC 3 cut(s) 175, 241, 324
LpnPI CCDG 7 cut(s) 233, 342, 395, 581, 636, 661, 726
Lsp1109I GCAGC 2 cut(s) 73, 698
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 480
MaeII ACGT 1 cut(s) 141
MalI GATC 3 cut(s) 177, 243, 326
MboI GATC 3 cut(s) 175, 241, 324
MboII GAAGA 6 cut(s) 133, 327, 506, 509, 532, 683
MflI RGATCY 2 cut(s) 241, 324
MhlI GDGCHC 2 cut(s) 151, 652
MluCI AATT 4 cut(s) 257, 376, 554, 587
MlyI GAGTC 1 cut(s) 278
MnlI CCTC 7 cut(s) 73, 104, 232, 256, 259, 639, 721
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 4 cut(s) 27, 254, 338, 723
MslI CAYNNNNRTG 1 cut(s) 144
MspCI CTTAAG 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 29
NcoI CCATGG 2 cut(s) 45, 454
NdeII GATC 3 cut(s) 175, 241, 324
NlaIII CATG 3 cut(s) 49, 182, 458
OliI CACNNNNGTG 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 408
PflMI CCANNNNNTGG 1 cut(s) 455
PkrI GCNGC 2 cut(s) 88, 688
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
PsiI TTATAA 1 cut(s) 332
PspFI CCCAGC 2 cut(s) 247, 381
PsuI RGATCY 2 cut(s) 241, 324
RseI CAYNNNNRTG 1 cut(s) 144
SaqAI TTAA 4 cut(s) 27, 254, 338, 723
SatI GCNGC 2 cut(s) 87, 687
Sau3AI GATC 3 cut(s) 175, 241, 324
SchI GAGTC 1 cut(s) 278
SduI GDGCHC 2 cut(s) 151, 652
SetI ASST 9 cut(s) 57, 84, 144, 280, 387, 600, 691, 715, 723
SfaNI GCATC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 3 cut(s) 236, 337, 698
SmoI CTYRAG 3 cut(s) 236, 337, 698
Sse9I AATT 4 cut(s) 257, 376, 554, 587
SspI AATATT 1 cut(s) 223
SspMI CTAG 1 cut(s) 480
StyI CCWWGG 2 cut(s) 45, 454
TaaI ACNGT 2 cut(s) 640, 749
TaiI ACGT 1 cut(s) 144
TaqI TCGA 2 cut(s) 5, 691
TasI AATT 4 cut(s) 257, 376, 554, 587
TfiI GAWTC 1 cut(s) 408
Tru1I TTAA 4 cut(s) 27, 254, 338, 723
Tru9I TTAA 4 cut(s) 27, 254, 338, 723
TscAI CASTG 1 cut(s) 151
TseI GCWGC 2 cut(s) 86, 686
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 1 cut(s) 151
Van91I CCANNNNNTGG 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 337
VneI GTGCAC 1 cut(s) 147
XagI CCTNNNNNAGG 1 cut(s) 725
XapI RAATTY 3 cut(s) 376, 554, 587
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.