AT1G01660

Modified RING finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0027316
Physical Location & Seq
Reverse (-)
239841 .. 242703
2863 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01660.1

Sequence Viewer

Length: 1707 bp
ATGGCGGAACTCATGGCCATGGGCAATGATGTTGTTCATGTTGCAGTGAAGAGTGATGTCAGAGAGAGCAGATCAACTCTTCTTTGGGCTTTGAGGAACCTCGGGGCTAAGAAAGTCTGCATCCTCCATGTTTATCAGCCAAAAACAGCTTCTCCGGCTGCTCGCAAGCTTGAAGAATTGGAGGCTATCATGTACGAGACATTACATGATTATTTTGATTTTTGTCAACAAGAAGGGGTGAATGAGGACGATATTTACATATCATGTATAGAGATGAACGATGTGAAGCAGGGGATACTAGAACTCATCCATGAGAGCAAAATCAAGAAGCTCGTCATGGGAGCTGCATCAGATCATCATTATTCAGAGAAGATGTTCGATCTCAAATCAAGAAAGGCAAAGTATGTTTACCAACACGCGCCCAGCTCGTGCGAGGTTATGTTTATGTGTGATGGACATCTCATCTACACAAAGGAAGCCAATCTTGAAGACTGTATGGGTGAAACAGAATCCGAAGCTGGACAATCCAAACCGAAGCTCTATTCCTCTGCTTCACCGAAATGCTCTGCAGAATTAGTCTCTGCAATTGTAGCTTACATTGACACAAGGAGAGACAGAGATATGTTGGAGCCAAATGCATCAGAGGATCAATCTGAAAGCGACAGGAATGATCAACTGTATAGACAGCTTAAGCAAGCACTTATGGAAGTTGAGGAATCGAAAAGGGAAGCTTACGAAGAATGTGTCAGGCGGTTTAAAGCTGAAAATACAGCCGTTGAGGCCATTCGCAGTGCAAGAGAGTACGAGGCCATGTACAACGAAGAGGCGAAGCTAAGGAAAGAAGGTAAAGAAGCATTAGCGAAGCAGAGGAAAATGGTGGAGAAGACTAAACAAGAGAGGGACGACGCGCTAATCATCATTCTAAATGGAAGGAAGCTATACAATGAGGAACTGAGACGCAGAGTTGAAGCTGAAGAGATGTTGGGGAAAGAGAAAGAAGAACACGAGAGAACTAAGAAGGAGATCGAAGAAGTGCGAGCCATTGTTCAGGATGGTACTCTGTACAACGAGCAGCTGAGACATAGGAAAGAGATGGAGGAATCTATGAAGAGACAAGAGGAAGAGCTTGAGAAGACAAAGAAGGAGAAGGAAGAAGCGTGCATGATAAGCAAGAACTTGATGCAGCTATATGAAGACGAGGTGAGGCAGAGGAAAGAGGCAGAGGAGTTGGTGAAGAGAAGAAGAGAGGAGCTAGAGAAAGTGAAGAAGGAGAAGGAAGAAGCTTGCTCTGTAGGACAAAACTTCATGAGGTTGTATGAAGAAGAAGCAAGACGTAGAAAAGGAACAGAGGAAGAACTCAGCAAGGTGGCGGCTGAGAAAGACGCAGCGAGCTCAGTCTGTTCGGAGATTCTATTACTCCTTCAGAGTTACACTCGTCGCCATGGAACTCCTTCTGGATTCTCCGATGAGGATTCCGTTACACGCCAGCCTCCCTCATATTTCATATGTCCTATCTCACAGGAAGTAATGCGAGAGCCTCGTGTTGCAGCCGATGGTTTCACCTATGAAGCTGAATCTTTAAGAGAATGGCTCGATAATGGTCACGAGACCTCACCAATGACAAACCTGAAGCTCGCCCACAACAACCTTGTCCCGAATCACGCCCTTCGTTCCGCGATTCAGGAGTGGCTACAACGAAACTCTTGA

Protein Analysis

568

Amino Acids

66.03

Weight (kDa)

5.4

Isoelectric Point (pI)

64.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 498 - 567 5.5e-19 U-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0027316)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01660 AT1G01660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 419, 908, 1676
AciI CCGC 4 cut(s) 5, 751, 1370, 1674
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 1 cut(s) 15
AcuI CTGAAG 3 cut(s) 993, 1406, 1649
AfaI GTAC 5 cut(s) 194, 803, 815, 1057, 1064
AflII CTTAAG 1 cut(s) 689
AgsI TTSAA 3 cut(s) 173, 488, 968
AjuI GAANNNNNNNTTGG 2 cut(s) 133, 165
Alw21I GWGCWC 1 cut(s) 1394
Alw26I GTCTC 7 cut(s) 191, 583, 606, 949, 1072, 1105, 1601
AlwI GGATC 1 cut(s) 654
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 3 cut(s) 15, 780, 807
ApeKI GCWGC 6 cut(s) 158, 344, 1072, 1183, 1385, 1547
ArsI GACNNNNNNTTYG 2 cut(s) 729, 761
Asp700I GAANNNNTTC 2 cut(s) 374, 1450
AspLEI GCGC 2 cut(s) 421, 910
AsuHPI GGTGA 7 cut(s) 250, 512, 546, 1213, 1243, 1552, 1605
AvaI CYCGRG 1 cut(s) 101
BalI TGGCCA 1 cut(s) 17
BanII GRGCYC 1 cut(s) 1394
BauI CACGAG 4 cut(s) 427, 1004, 1539, 1604
BbsI GAAGAC 4 cut(s) 495, 890, 1139, 1200
Bbv12I GWGCWC 1 cut(s) 1394
BbvI GCAGC 6 cut(s) 145, 331, 1084, 1195, 1397, 1559
BccI CCATC 4 cut(s) 446, 1046, 1087, 1547
BceAI ACGGC 1 cut(s) 758
BciVI GTATCC 1 cut(s) 288
BclI TGATCA 1 cut(s) 670
BcoDI GTCTC 7 cut(s) 191, 583, 606, 949, 1072, 1105, 1601
BfaI CTAG 2 cut(s) 299, 1253
BfmI CTRYAG 2 cut(s) 567, 1290
BfrI CTTAAG 1 cut(s) 689
BfuI GTATCC 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 779
BisI GCNGC 7 cut(s) 159, 345, 1073, 1184, 1371, 1386, 1548
BlsI GCNGC 7 cut(s) 160, 346, 1074, 1185, 1372, 1387, 1549
BmeT110I CYCGRG 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 98, 630
BmsI GCATC 4 cut(s) 129, 356, 647, 1170
BpiI GAAGAC 4 cut(s) 495, 890, 1139, 1200
BplI GAGNNNNNCTC 4 cut(s) 1417, 1449, 1575, 1607
Bpu10I CCTNAGC 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 1148
BsaBI GATNNNNATC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 1601
BsaJI CCNNGG 3 cut(s) 18, 100, 1441
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bse3DI GCAATG 1 cut(s) 31
Bse8I GATNNNNATC 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 18, 100, 1441
BseGI GGATG 3 cut(s) 120, 306, 1057
BseJI GATNNNNATC 1 cut(s) 456
BseMI GCAATG 1 cut(s) 31
BseMII CTCAG 5 cut(s) 944, 1067, 1365, 1372, 1407
BseRI GAGGAG 2 cut(s) 1238, 1262
BseXI GCAGC 6 cut(s) 145, 331, 1084, 1195, 1397, 1559
BseYI CCCAGC 1 cut(s) 422
Bsh1236I CGCG 3 cut(s) 419, 908, 1676
BshFI GGCC 3 cut(s) 17, 782, 809
BsiHKAI GWGCWC 1 cut(s) 1394
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 1 cut(s) 155
BslFI GGGAC 2 cut(s) 914, 1637
BsmAI GTCTC 7 cut(s) 191, 583, 606, 949, 1072, 1105, 1601
BsmBI CGTCTC 1 cut(s) 949
BsmFI GGGAC 2 cut(s) 914, 1637
BsnI GGCC 3 cut(s) 17, 782, 809
Bso31I GGTCTC 1 cut(s) 1601
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 1394
Bsp1407I TGTACA 2 cut(s) 813, 1062
Bsp143I GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
Bsp19I CCATGG 2 cut(s) 18, 1441
BspACI CCGC 4 cut(s) 5, 751, 1370, 1674
BspANI GGCC 3 cut(s) 17, 782, 809
BspCNI CTCAG 5 cut(s) 945, 1068, 1366, 1371, 1406
BspFNI CGCG 3 cut(s) 419, 908, 1676
BspHI TCATGA 1 cut(s) 1305
BspLI GGNNCC 2 cut(s) 98, 630
BspMAI CTGCAG 1 cut(s) 571
BspPI GGATC 1 cut(s) 654
BspQI GCTCTTC 1 cut(s) 1116
BspTI CTTAAG 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 1601
BsrDI GCAATG 1 cut(s) 31
BsrGI TGTACA 2 cut(s) 813, 1062
BssECI CCNNGG 3 cut(s) 18, 100, 1441
BssMI GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
BssSI CACGAG 4 cut(s) 427, 1004, 1539, 1604
BssT1I CCWWGG 2 cut(s) 18, 1441
Bst2BI CACGAG 4 cut(s) 427, 1004, 1539, 1604
Bst4CI ACNGT 2 cut(s) 494, 678
Bst6I CTCTTC 8 cut(s) 44, 84, 816, 969, 1103, 1116, 1229, 1237
BstAFI CTTAAG 1 cut(s) 689
BstAUI TGTACA 2 cut(s) 813, 1062
BstC8I GCNNGC 9 cut(s) 163, 167, 696, 1038, 1159, 1285, 1390, 1487, 1635
BstDEI CTNAG 8 cut(s) 108, 833, 953, 1014, 1076, 1358, 1374, 1393
BstDSI CCRYGG 2 cut(s) 18, 1441
BstF5I GGATG 3 cut(s) 120, 306, 1057
BstFNI CGCG 3 cut(s) 419, 908, 1676
BstHHI GCGC 2 cut(s) 421, 910
BstKTI GATC 6 cut(s) 74, 355, 382, 649, 673, 1026
BstMAI GTCTC 7 cut(s) 191, 583, 606, 949, 1072, 1105, 1601
BstMBI GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
BstMWI GCNNNNNNNGC 4 cut(s) 155, 590, 779, 1167
BstSFI CTRYAG 2 cut(s) 567, 1290
BstUI CGCG 3 cut(s) 419, 908, 1676
BstV1I GCAGC 6 cut(s) 145, 331, 1084, 1195, 1397, 1559
BstV2I GAAGAC 4 cut(s) 495, 890, 1139, 1200
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 3 cut(s) 17, 782, 809
BtgI CCRYGG 2 cut(s) 18, 1441
BtsCI GGATG 3 cut(s) 120, 306, 1057
BtsI GCAGTG 2 cut(s) 51, 796
BtsIMutI CAGTG 2 cut(s) 51, 796
Cac8I GCNNGC 9 cut(s) 163, 167, 696, 1038, 1159, 1285, 1390, 1487, 1635
CciI TCATGA 1 cut(s) 1305
CfoI GCGC 2 cut(s) 421, 910
CseI GACGC 3 cut(s) 914, 966, 1391
Csp6I GTAC 5 cut(s) 193, 802, 814, 1056, 1063
CviQI GTAC 5 cut(s) 193, 802, 814, 1056, 1063
DdeI CTNAG 8 cut(s) 108, 833, 953, 1014, 1076, 1358, 1374, 1393
DpnI GATC 6 cut(s) 73, 354, 381, 648, 672, 1025
DpnII GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
DraI TTTAAA 1 cut(s) 757
EaeI YGGCCR 1 cut(s) 15
Eam1104I CTCTTC 8 cut(s) 44, 84, 816, 969, 1103, 1116, 1229, 1237
EarI CTCTTC 8 cut(s) 44, 84, 816, 969, 1103, 1116, 1229, 1237
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 1392
Eco130I CCWWGG 2 cut(s) 18, 1441
Eco24I GRGCYC 1 cut(s) 1394
Eco31I GGTCTC 1 cut(s) 1601
Eco53kI GAGCTC 1 cut(s) 1392
Eco57I CTGAAG 3 cut(s) 993, 1406, 1649
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 1392
EcoT14I CCWWGG 2 cut(s) 18, 1441
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 1394
ErhI CCWWGG 2 cut(s) 18, 1441
Esp3I CGTCTC 1 cut(s) 949
FalI AAGNNNNNCTT 4 cut(s) 468, 500, 715, 747
FaqI GGGAC 2 cut(s) 914, 1637
FauNDI CATATG 1 cut(s) 1505
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 7 cut(s) 159, 345, 1073, 1184, 1371, 1386, 1548
FokI GGATG 3 cut(s) 107, 293, 1064
FriOI GRGCYC 1 cut(s) 1394
Fsp4HI GCNGC 7 cut(s) 159, 345, 1073, 1184, 1371, 1386, 1548
FspBI CTAG 2 cut(s) 299, 1253
GlaI GCGC 2 cut(s) 420, 909
GluI GCNGC 7 cut(s) 159, 345, 1073, 1184, 1371, 1386, 1548
GsaI CCCAGC 1 cut(s) 426
HaeIII GGCC 3 cut(s) 17, 782, 809
HapII CCGG 1 cut(s) 155
HgaI GACGC 3 cut(s) 914, 966, 1391
HhaI GCGC 2 cut(s) 421, 910
Hin6I GCGC 2 cut(s) 419, 908
HinP1I GCGC 2 cut(s) 419, 908
HincII GTYRAC 1 cut(s) 227
HindII GTYRAC 1 cut(s) 227
HindIII AAGCTT 3 cut(s) 167, 729, 1281
HinfI GANTC 9 cut(s) 509, 716, 1100, 1408, 1458, 1472, 1574, 1657, 1678
HpaII CCGG 1 cut(s) 155
HphI GGTGA 7 cut(s) 250, 512, 546, 1213, 1243, 1552, 1605
Hpy166II GTNNAC 2 cut(s) 227, 409
Hpy188I TCNGA 9 cut(s) 62, 352, 367, 514, 643, 655, 1405, 1425, 1465
Hpy8I GTNNAC 2 cut(s) 227, 409
Hpy99I CGWCG 2 cut(s) 908, 1440
HpyCH4III ACNGT 2 cut(s) 494, 678
HpyCH4IV ACGT 1 cut(s) 1333
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 590, 779, 1167
HpyF3I CTNAG 8 cut(s) 108, 833, 953, 1014, 1076, 1358, 1374, 1393
HpySE526I ACGT 1 cut(s) 1333
HspAI GCGC 2 cut(s) 419, 908
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
LguI GCTCTTC 1 cut(s) 1116
LmnI GCTCC 3 cut(s) 341, 628, 1249
Lsp1109I GCAGC 6 cut(s) 145, 331, 1084, 1195, 1397, 1559
LweI GCATC 4 cut(s) 129, 356, 647, 1170
MaeI CTAG 2 cut(s) 299, 1253
MaeII ACGT 1 cut(s) 1333
MaeIII GTNAC 3 cut(s) 1427, 1477, 1601
MalI GATC 6 cut(s) 73, 354, 381, 648, 672, 1025
MboI GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
MfeI CAATTG 1 cut(s) 585
MhlI GDGCHC 1 cut(s) 1394
MlsI TGGCCA 1 cut(s) 17
MluCI AATT 3 cut(s) 176, 572, 585
MluNI TGGCCA 1 cut(s) 17
MmeI TCCRAC 1 cut(s) 606
Mox20I TGGCCA 1 cut(s) 17
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 2 cut(s) 374, 1450
MscI TGGCCA 1 cut(s) 17
MseI TTAA 3 cut(s) 690, 756, 1580
MslI CAYNNNNRTG 2 cut(s) 17, 559
Msp20I TGGCCA 1 cut(s) 17
MspA1I CMGCKG 1 cut(s) 1075
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 1 cut(s) 155
MunI CAATTG 1 cut(s) 585
MvnI CGCG 3 cut(s) 419, 908, 1676
MwoI GCNNNNNNNGC 4 cut(s) 155, 590, 779, 1167
NcoI CCATGG 2 cut(s) 18, 1441
NdeI CATATG 1 cut(s) 1505
NdeII GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
NlaIV GGNNCC 2 cut(s) 98, 630
NmuCI GTSAC 1 cut(s) 1601
NsiI ATGCAT 1 cut(s) 640
PagI TCATGA 1 cut(s) 1305
PciSI GCTCTTC 1 cut(s) 1116
PdmI GAANNNNTTC 2 cut(s) 374, 1450
PfeI GAWTC 9 cut(s) 509, 716, 1100, 1408, 1458, 1472, 1574, 1657, 1678
PkrI GCNGC 7 cut(s) 160, 346, 1074, 1185, 1372, 1387, 1549
Psp124BI GAGCTC 1 cut(s) 1394
PspFI CCCAGC 1 cut(s) 422
PspN4I GGNNCC 2 cut(s) 98, 630
PstI CTGCAG 1 cut(s) 571
PvuII CAGCTG 1 cut(s) 1075
RsaI GTAC 5 cut(s) 194, 803, 815, 1057, 1064
RsaNI GTAC 5 cut(s) 193, 802, 814, 1056, 1063
RseI CAYNNNNRTG 2 cut(s) 17, 559
SacI GAGCTC 1 cut(s) 1394
SapI GCTCTTC 1 cut(s) 1116
SaqAI TTAA 3 cut(s) 690, 756, 1580
SatI GCNGC 7 cut(s) 159, 345, 1073, 1184, 1371, 1386, 1548
Sau3AI GATC 6 cut(s) 71, 352, 379, 646, 670, 1023
SduI GDGCHC 1 cut(s) 1394
SfaNI GCATC 4 cut(s) 129, 356, 647, 1170
SfcI CTRYAG 2 cut(s) 567, 1290
SmiMI CAYNNNNRTG 2 cut(s) 17, 559
SmlI CTYRAG 2 cut(s) 689, 1127
SmoI CTYRAG 2 cut(s) 689, 1127
Sse9I AATT 3 cut(s) 176, 572, 585
SsiI CCGC 4 cut(s) 5, 751, 1370, 1674
SspMI CTAG 2 cut(s) 299, 1253
SstI GAGCTC 1 cut(s) 1394
StyI CCWWGG 2 cut(s) 18, 1441
TaaI ACNGT 2 cut(s) 494, 678
TaiI ACGT 1 cut(s) 1336
TaqI TCGA 4 cut(s) 378, 719, 1026, 1593
TasI AATT 3 cut(s) 176, 572, 585
TatI WGTACW 2 cut(s) 813, 1062
TauI GCSGC 1 cut(s) 1373
TfiI GAWTC 9 cut(s) 509, 716, 1100, 1408, 1458, 1472, 1574, 1657, 1678
Tru1I TTAA 3 cut(s) 690, 756, 1580
Tru9I TTAA 3 cut(s) 690, 756, 1580
TscAI CASTG 2 cut(s) 51, 796
TseFI GTSAC 1 cut(s) 1601
TseI GCWGC 6 cut(s) 158, 344, 1072, 1183, 1385, 1547
Tsp45I GTSAC 1 cut(s) 1601
TspDTI ATGAA 8 cut(s) 26, 290, 1121, 1206, 1294, 1332, 1492, 1581
TspGWI ACGGA 1 cut(s) 1465
TspRI CASTG 2 cut(s) 51, 796
Vha464I CTTAAG 1 cut(s) 689
XmnI GAANNNNTTC 2 cut(s) 374, 1450
XspI CTAG 2 cut(s) 299, 1253
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.