AT1G06460

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0023786
Physical Location & Seq
Reverse (-)
1967087 .. 1969656
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06460.1

Sequence Viewer

Length: 858 bp
ATGGAGCATGAATCTATCACCGCTCGACGCAGGCTCGCTGCCTTCGCCGCTCACTTCCCGGCAACCAGTTACGATTCCGCCTCCACCGCATCTCTCGTCCCCTTGAACTGCAGCAGCAGTTTGAACACTGTCATCCAAAGGTGTGACAACAAAATCTCTTTTGCCCGGCAAGCATCATCTGAACAGGGCTTTTTTATGAGGCCAGCTTCACCCGATGATGTCCTTGAGAACTTAGGTATGAATTTGAAGAACACTGTCGTTAGAAGGGGTGACAACAGGCTGTATTTTGCTCGGCAAGCATCTTCTGCACAAGGTTTTTTTATGAGACAGGCTTCGACTAATGAGAGGACCATACCACATGATGCTGCTGCATCTACCAAGTTTTCTGCTACCAAAACAACTGGCTTCGATTCTTCTTCACCTGCGTACGCTGCACCACACTTTTCTAAGCCTGCAAAAGAGGACATCTTTTTTCCTTCTTTATCACCAAATCTGCAAAAGGAAAGGCCTAAGCTTGATCTTCCTAAGTTAGCCAATTTAGGTACTGTTTGGTCTCCCAGATCGAATGTTGCTGAATCGACACACAGCTATGTTGTGGCAATAGAGCTACCAGGAGCCAGTATCAATGATATAAGAGTGGAGGTTGACAACACAAACTTGACTGTGACAGGTAGACGCACATCTATCTGTCAAAAAGTTGATGCAGGCACCAAAGCTTCGATCCTTGGATATCATAAGCAAGAAATACTACAAGGACCATTCAAAGTTTCCTGGCCACTCCCCAGCAATGTGAACAAGGATAATGTTTCCGCTGAATTTATGGATGGGATTCTGAGAATAGTAATTCCAAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

285

Amino Acids

31.22

Weight (kDa)

9.38

Isoelectric Point (pI)

49.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 191 - 284 8.4e-13 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023786)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06460
prunus_persica Prupe.3G108500_v2.0.a1 Prupe.3G108500_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 430
Acc36I ACCTGC 1 cut(s) 430
AccB1I GGYRCC 1 cut(s) 707
AccBSI CCGCTC 2 cut(s) 23, 50
AccI GTMKAC 1 cut(s) 673
AciI CCGC 5 cut(s) 21, 48, 78, 87, 810
AclWI GGATC 1 cut(s) 715
AcoI YGGCCR 1 cut(s) 773
AcsI RAATTY 2 cut(s) 241, 815
AfaI GTAC 2 cut(s) 428, 544
AgsI TTSAA 4 cut(s) 106, 124, 247, 763
AjnI CCWGG 2 cut(s) 610, 770
AluBI AGCT 6 cut(s) 206, 514, 588, 607, 716, 853
AluI AGCT 6 cut(s) 206, 514, 588, 607, 716, 853
Alw26I GTCTC 2 cut(s) 319, 558
AlwI GGATC 1 cut(s) 715
AoxI GGCC 3 cut(s) 200, 506, 773
ApeKI GCWGC 6 cut(s) 38, 111, 114, 365, 368, 431
ApoI RAATTY 2 cut(s) 241, 815
AspS9I GGNCC 2 cut(s) 348, 755
AsuC2I CCSGG 2 cut(s) 59, 166
AsuHPI GGTGA 5 cut(s) 10, 201, 281, 411, 477
AvaII GGWCC 2 cut(s) 348, 755
BalI TGGCCA 1 cut(s) 775
BanI GGYRCC 1 cut(s) 707
BbvI GCAGC 6 cut(s) 25, 123, 126, 352, 355, 418
BccI CCATC 1 cut(s) 818
BciT130I CCWGG 2 cut(s) 612, 772
BcnI CCSGG 2 cut(s) 59, 166
BcoDI GTCTC 2 cut(s) 319, 558
BfmI CTRYAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 430
BisI GCNGC 7 cut(s) 39, 48, 112, 115, 366, 369, 432
BlsI GCNGC 7 cut(s) 40, 49, 113, 116, 367, 370, 433
Bme1390I CCNGG 4 cut(s) 59, 166, 612, 772
Bme18I GGWCC 2 cut(s) 348, 755
BmgT120I GGNCC 2 cut(s) 348, 755
BmiI GGNNCC 2 cut(s) 616, 709
BmrFI CCNGG 4 cut(s) 59, 166, 612, 772
BmsI GCATC 6 cut(s) 98, 182, 308, 352, 380, 691
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 59, 166
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 1 cut(s) 724
Bse1I ACTGG 3 cut(s) 66, 406, 618
Bse3DI GCAATG 1 cut(s) 793
BseBI CCWGG 2 cut(s) 612, 772
BseDI CCNNGG 1 cut(s) 724
BseGI GGATG 2 cut(s) 132, 829
BseMI GCAATG 1 cut(s) 793
BseMII CTCAG 1 cut(s) 824
BseNI ACTGG 3 cut(s) 66, 406, 618
BseXI GCAGC 6 cut(s) 25, 123, 126, 352, 355, 418
BseYI CCCAGC 1 cut(s) 782
BsgI GTGCAG 2 cut(s) 291, 417
BshFI GGCC 3 cut(s) 202, 508, 775
BshNI GGYRCC 1 cut(s) 707
BsiSI CCGG 2 cut(s) 59, 166
BsiWI CGTACG 1 cut(s) 426
BslFI GGGAC 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 319, 558
BsmFI GGGAC 1 cut(s) 83
BsnI GGCC 3 cut(s) 202, 508, 775
Bso31I GGTCTC 1 cut(s) 558
Bsp143I GATC 3 cut(s) 517, 560, 720
BspACI CCGC 5 cut(s) 21, 48, 78, 87, 810
BspANI GGCC 3 cut(s) 202, 508, 775
BspCNI CTCAG 1 cut(s) 825
BspLI GGNNCC 2 cut(s) 616, 709
BspMAI CTGCAG 1 cut(s) 113
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 1 cut(s) 715
BspT107I GGYRCC 1 cut(s) 707
BspTNI GGTCTC 1 cut(s) 558
BsrBI CCGCTC 2 cut(s) 23, 50
BsrDI GCAATG 1 cut(s) 793
BsrI ACTGG 3 cut(s) 66, 406, 618
BssECI CCNNGG 1 cut(s) 724
BssMI GATC 3 cut(s) 517, 560, 720
BssT1I CCWWGG 1 cut(s) 724
Bst2UI CCWGG 2 cut(s) 612, 772
Bst4CI ACNGT 4 cut(s) 130, 256, 547, 664
BstAPI GCANNNNNTGC 1 cut(s) 305
BstC8I GCNNGC 7 cut(s) 32, 36, 171, 204, 297, 453, 706
BstDEI CTNAG 5 cut(s) 232, 447, 510, 525, 833
BstF5I GGATG 2 cut(s) 132, 829
BstKTI GATC 3 cut(s) 520, 563, 723
BstMAI GTCTC 2 cut(s) 319, 558
BstMBI GATC 3 cut(s) 517, 560, 720
BstMWI GCNNNNNNNGC 7 cut(s) 44, 47, 86, 170, 296, 305, 431
BstNI CCWGG 2 cut(s) 612, 772
BstSCI CCNGG 4 cut(s) 57, 164, 610, 770
BstSFI CTRYAG 1 cut(s) 109
BstV1I GCAGC 6 cut(s) 25, 123, 126, 352, 355, 418
BsuRI GGCC 3 cut(s) 202, 508, 775
BtsCI GGATG 2 cut(s) 132, 829
BtsIMutI CAGTG 2 cut(s) 126, 252
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 7 cut(s) 32, 36, 171, 204, 297, 453, 706
Cfr13I GGNCC 2 cut(s) 348, 755
CseI GACGC 2 cut(s) 36, 684
Csp6I GTAC 2 cut(s) 427, 543
CviAII CATG 2 cut(s) 8, 359
CviQI GTAC 2 cut(s) 427, 543
DdeI CTNAG 5 cut(s) 232, 447, 510, 525, 833
DpnI GATC 3 cut(s) 519, 562, 722
DpnII GATC 3 cut(s) 517, 560, 720
EaeI YGGCCR 1 cut(s) 773
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 724
Eco147I AGGCCT 1 cut(s) 508
Eco31I GGTCTC 1 cut(s) 558
Eco32I GATATC 1 cut(s) 731
Eco47I GGWCC 2 cut(s) 348, 755
EcoRII CCWGG 2 cut(s) 610, 770
EcoRV GATATC 1 cut(s) 731
EcoT14I CCWWGG 1 cut(s) 724
ErhI CCWWGG 1 cut(s) 724
FaeI CATG 2 cut(s) 11, 362
FaqI GGGAC 1 cut(s) 83
FatI CATG 2 cut(s) 7, 358
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 7 cut(s) 39, 48, 112, 115, 366, 369, 432
FokI GGATG 2 cut(s) 119, 836
Fsp4HI GCNGC 7 cut(s) 39, 48, 112, 115, 366, 369, 432
GluI GCNGC 7 cut(s) 39, 48, 112, 115, 366, 369, 432
GsaI CCCAGC 1 cut(s) 786
HaeIII GGCC 3 cut(s) 202, 508, 775
HapII CCGG 2 cut(s) 59, 166
HgaI GACGC 2 cut(s) 36, 684
Hin1II CATG 2 cut(s) 11, 362
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HindIII AAGCTT 3 cut(s) 512, 714, 851
HinfI GANTC 5 cut(s) 11, 74, 410, 575, 829
HpaII CCGG 2 cut(s) 59, 166
HphI GGTGA 5 cut(s) 10, 201, 281, 411, 477
Hpy166II GTNNAC 3 cut(s) 646, 674, 793
Hpy188I TCNGA 2 cut(s) 181, 834
Hpy8I GTNNAC 3 cut(s) 646, 674, 793
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 3 cut(s) 52, 258, 486
HpyCH4III ACNGT 4 cut(s) 130, 256, 547, 664
HpyCH4V TGCA 7 cut(s) 111, 308, 371, 434, 455, 496, 704
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 47, 86, 170, 296, 305, 431
HpyF3I CTNAG 5 cut(s) 232, 447, 510, 525, 833
Hsp92II CATG 2 cut(s) 11, 362
Kzo9I GATC 3 cut(s) 517, 560, 720
LmnI GCTCC 2 cut(s) 4, 614
Lsp1109I GCAGC 6 cut(s) 25, 123, 126, 352, 355, 418
LweI GCATC 6 cut(s) 98, 182, 308, 352, 380, 691
MaeIII GTNAC 4 cut(s) 68, 143, 269, 664
MalI GATC 3 cut(s) 519, 562, 722
MbiI CCGCTC 2 cut(s) 23, 50
MboI GATC 3 cut(s) 517, 560, 720
MboII GAAGA 5 cut(s) 259, 294, 405, 408, 512
MlsI TGGCCA 1 cut(s) 775
MluCI AATT 4 cut(s) 241, 535, 815, 843
MluNI TGGCCA 1 cut(s) 775
MnlI CCTC 5 cut(s) 91, 192, 339, 454, 634
Mox20I TGGCCA 1 cut(s) 775
MscI TGGCCA 1 cut(s) 775
MslI CAYNNNNRTG 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 775
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 2 cut(s) 59, 166
MspR9I CCNGG 4 cut(s) 59, 166, 612, 772
MvaI CCWGG 2 cut(s) 612, 772
MwoI GCNNNNNNNGC 7 cut(s) 44, 47, 86, 170, 296, 305, 431
NciI CCSGG 2 cut(s) 59, 166
NdeII GATC 3 cut(s) 517, 560, 720
NlaIII CATG 2 cut(s) 11, 362
NlaIV GGNNCC 2 cut(s) 616, 709
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 3 cut(s) 143, 269, 664
PaqCI CACCTGC 1 cut(s) 430
PceI AGGCCT 1 cut(s) 508
PfeI GAWTC 5 cut(s) 11, 74, 410, 575, 829
Pfl23II CGTACG 1 cut(s) 426
PkrI GCNGC 7 cut(s) 40, 49, 113, 116, 367, 370, 433
Psp6I CCWGG 2 cut(s) 610, 770
PspFI CCCAGC 1 cut(s) 782
PspGI CCWGG 2 cut(s) 610, 770
PspLI CGTACG 1 cut(s) 426
PspN4I GGNNCC 2 cut(s) 616, 709
PspPI GGNCC 2 cut(s) 348, 755
PstI CTGCAG 1 cut(s) 113
RsaI GTAC 2 cut(s) 428, 544
RsaNI GTAC 2 cut(s) 427, 543
RseI CAYNNNNRTG 1 cut(s) 588
SatI GCNGC 7 cut(s) 39, 48, 112, 115, 366, 369, 432
Sau3AI GATC 3 cut(s) 517, 560, 720
Sau96I GGNCC 2 cut(s) 348, 755
ScrFI CCNGG 4 cut(s) 59, 166, 612, 772
SfaNI GCATC 6 cut(s) 98, 182, 308, 352, 380, 691
SfcI CTRYAG 1 cut(s) 109
SinI GGWCC 2 cut(s) 348, 755
SmiMI CAYNNNNRTG 1 cut(s) 588
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
Sse9I AATT 4 cut(s) 241, 535, 815, 843
SseBI AGGCCT 1 cut(s) 508
SsiI CCGC 5 cut(s) 21, 48, 78, 87, 810
StuI AGGCCT 1 cut(s) 508
StyD4I CCNGG 4 cut(s) 57, 164, 610, 770
StyI CCWWGG 1 cut(s) 724
TaaI ACNGT 4 cut(s) 130, 256, 547, 664
TaqI TCGA 6 cut(s) 25, 335, 408, 563, 578, 719
TasI AATT 4 cut(s) 241, 535, 815, 843
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 5 cut(s) 11, 74, 410, 575, 829
TscAI CASTG 2 cut(s) 133, 259
TseFI GTSAC 3 cut(s) 143, 269, 664
TseI GCWGC 6 cut(s) 38, 111, 114, 365, 368, 431
Tsp45I GTSAC 3 cut(s) 143, 269, 664
TspDTI ATGAA 2 cut(s) 24, 254
TspRI CASTG 2 cut(s) 133, 259
VpaK11BI GGWCC 2 cut(s) 348, 755
XapI RAATTY 2 cut(s) 241, 815
XmiI GTMKAC 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.