Leucine rich repeat
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 740, 1323 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 292 |
| AccBSI | CCGCTC | 2 cut(s) | 1406, 1988 |
| AccI | GTMKAC | 1 cut(s) | 1533 |
| AccII | CGCG | 1 cut(s) | 1656 |
| AclWI | GGATC | 7 cut(s) | 630, 761, 929, 1003, 1741, 1767, 2165 |
| AcoI | YGGCCR | 1 cut(s) | 1047 |
| AcsI | RAATTY | 5 cut(s) | 693, 1004, 1429, 1616, 1873 |
| AcuI | CTGAAG | 5 cut(s) | 283, 969, 1026, 1305, 1454 |
| AdeI | CACNNNGTG | 1 cut(s) | 1607 |
| AfaI | GTAC | 3 cut(s) | 1721, 1794, 1917 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 292, 821, 986, 1192, 1193, 1232 |
| AflII | CTTAAG | 2 cut(s) | 253, 566 |
| AflIII | ACRYGT | 1 cut(s) | 1918 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 511 |
| AjnI | CCWGG | 3 cut(s) | 229, 938, 2251 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 12, 44, 1506, 1538 |
| Alw21I | GWGCWC | 3 cut(s) | 130, 793, 1135 |
| Alw26I | GTCTC | 5 cut(s) | 38, 156, 546, 1242, 1636 |
| AlwI | GGATC | 7 cut(s) | 630, 761, 929, 1003, 1741, 1767, 2165 |
| Ama87I | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| AoxI | GGCC | 2 cut(s) | 1047, 2093 |
| ApeKI | GCWGC | 2 cut(s) | 481, 1354 |
| ApoI | RAATTY | 5 cut(s) | 693, 1004, 1429, 1616, 1873 |
| AseI | ATTAAT | 1 cut(s) | 102 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1201 |
| AspS9I | GGNCC | 2 cut(s) | 668, 1011 |
| AsuC2I | CCSGG | 2 cut(s) | 1421, 1422 |
| AsuHPI | GGTGA | 7 cut(s) | 776, 879, 897, 969, 1447, 1582, 1859 |
| AvaI | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| AvaII | GGWCC | 2 cut(s) | 668, 1011 |
| AxyI | CCTNAGG | 1 cut(s) | 1992 |
| BalI | TGGCCA | 1 cut(s) | 1049 |
| BanII | GRGCYC | 1 cut(s) | 1135 |
| BbsI | GAAGAC | 1 cut(s) | 1875 |
| Bbv12I | GWGCWC | 3 cut(s) | 130, 793, 1135 |
| BbvI | GCAGC | 2 cut(s) | 493, 1341 |
| BceAI | ACGGC | 1 cut(s) | 1627 |
| BciT130I | CCWGG | 3 cut(s) | 231, 940, 2253 |
| BciVI | GTATCC | 5 cut(s) | 219, 818, 1075, 1275, 2202 |
| BcnI | CCSGG | 2 cut(s) | 1421, 1422 |
| BcoDI | GTCTC | 5 cut(s) | 38, 156, 546, 1242, 1636 |
| BfaI | CTAG | 3 cut(s) | 1031, 1628, 2114 |
| BfmI | CTRYAG | 2 cut(s) | 1534, 2240 |
| BfrI | CTTAAG | 2 cut(s) | 253, 566 |
| BfuI | GTATCC | 5 cut(s) | 219, 818, 1075, 1275, 2202 |
| BglII | AGATCT | 1 cut(s) | 2287 |
| BisI | GCNGC | 5 cut(s) | 482, 1352, 1355, 1407, 1986 |
| BlpI | GCTNAGC | 2 cut(s) | 1542, 1580 |
| BlsI | GCNGC | 5 cut(s) | 483, 1353, 1356, 1408, 1987 |
| BmcAI | AGTACT | 1 cut(s) | 1721 |
| Bme1390I | CCNGG | 5 cut(s) | 231, 940, 1421, 1422, 2253 |
| Bme18I | GGWCC | 2 cut(s) | 668, 1011 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 511 |
| BmeT110I | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| BmgT120I | GGNCC | 2 cut(s) | 668, 1011 |
| BmiI | GGNNCC | 3 cut(s) | 669, 1212, 2250 |
| BmrFI | CCNGG | 5 cut(s) | 231, 940, 1421, 1422, 2253 |
| BmrI | ACTGGG | 1 cut(s) | 393 |
| BmsI | GCATC | 2 cut(s) | 1735, 2140 |
| BmuI | ACTGGG | 1 cut(s) | 393 |
| BpiI | GAAGAC | 1 cut(s) | 1875 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1393, 1425 |
| BpmI | CTGGAG | 3 cut(s) | 90, 545, 1266 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 1542, 1580 |
| BpuEI | CTTGAG | 1 cut(s) | 352 |
| BpuMI | CCSGG | 2 cut(s) | 1421, 1422 |
| Bsa29I | ATCGAT | 2 cut(s) | 734, 2148 |
| BsaAI | YACGTR | 2 cut(s) | 1796, 1919 |
| BsaI | GGTCTC | 2 cut(s) | 38, 156 |
| BsaJI | CCNNGG | 9 cut(s) | 546, 938, 1367, 1419, 1420, 1887, 2038, 2096, 2132 |
| BsaWI | WCCGGW | 1 cut(s) | 1067 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 292, 821, 986, 1192, 1193, 1232 |
| Bse118I | RCCGGY | 1 cut(s) | 1858 |
| Bse1I | ACTGG | 5 cut(s) | 388, 507, 528, 1378, 2071 |
| Bse21I | CCTNAGG | 1 cut(s) | 1992 |
| Bse3DI | GCAATG | 3 cut(s) | 1308, 1458, 1826 |
| BseBI | CCWGG | 3 cut(s) | 231, 940, 2253 |
| BseCI | ATCGAT | 2 cut(s) | 734, 2148 |
| BseDI | CCNNGG | 9 cut(s) | 546, 938, 1367, 1419, 1420, 1887, 2038, 2096, 2132 |
| BseGI | GGATG | 3 cut(s) | 348, 874, 1336 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 292, 821, 986, 1192, 1193, 1232 |
| BseMI | GCAATG | 3 cut(s) | 1308, 1458, 1826 |
| BseMII | CTCAG | 7 cut(s) | 78, 1506, 1533, 1571, 1926, 1983, 2132 |
| BseNI | ACTGG | 5 cut(s) | 388, 507, 528, 1378, 2071 |
| BseRI | GAGGAG | 6 cut(s) | 174, 193, 762, 783, 942, 945 |
| BseXI | GCAGC | 2 cut(s) | 493, 1341 |
| BseYI | CCCAGC | 1 cut(s) | 832 |
| Bsh1236I | CGCG | 1 cut(s) | 1656 |
| BshFI | GGCC | 2 cut(s) | 1049, 2095 |
| BshVI | ATCGAT | 2 cut(s) | 734, 2148 |
| BsiHKAI | GWGCWC | 3 cut(s) | 130, 793, 1135 |
| BsiHKCI | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| BsiSI | CCGG | 4 cut(s) | 1068, 1294, 1421, 1859 |
| BslFI | GGGAC | 3 cut(s) | 334, 681, 2115 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 292, 821, 986, 1192, 1193, 1232 |
| BsmAI | GTCTC | 5 cut(s) | 38, 156, 546, 1242, 1636 |
| BsmFI | GGGAC | 3 cut(s) | 334, 681, 2115 |
| BsnI | GGCC | 2 cut(s) | 1049, 2095 |
| Bso31I | GGTCTC | 2 cut(s) | 38, 156 |
| BsoBI | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| Bsp1286I | GDGCHC | 3 cut(s) | 130, 793, 1135 |
| Bsp143I | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 1542, 1580 |
| Bsp19I | CCATGG | 1 cut(s) | 546 |
| BspANI | GGCC | 2 cut(s) | 1049, 2095 |
| BspCNI | CTCAG | 7 cut(s) | 77, 1505, 1534, 1572, 1925, 1984, 2131 |
| BspDI | ATCGAT | 2 cut(s) | 734, 2148 |
| BspFNI | CGCG | 1 cut(s) | 1656 |
| BspHI | TCATGA | 2 cut(s) | 1840, 2140 |
| BspLI | GGNNCC | 3 cut(s) | 669, 1212, 2250 |
| BspMAI | CTGCAG | 1 cut(s) | 2244 |
| BspPI | GGATC | 7 cut(s) | 630, 761, 929, 1003, 1741, 1767, 2165 |
| BspTI | CTTAAG | 2 cut(s) | 253, 566 |
| BspTNI | GGTCTC | 2 cut(s) | 38, 156 |
| BsrBI | CCGCTC | 2 cut(s) | 1406, 1988 |
| BsrDI | GCAATG | 3 cut(s) | 1308, 1458, 1826 |
| BsrFI | RCCGGY | 1 cut(s) | 1858 |
| BsrI | ACTGG | 5 cut(s) | 388, 507, 528, 1378, 2071 |
| BssAI | RCCGGY | 1 cut(s) | 1858 |
| BssECI | CCNNGG | 9 cut(s) | 546, 938, 1367, 1419, 1420, 1887, 2038, 2096, 2132 |
| BssMI | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| BssT1I | CCWWGG | 3 cut(s) | 546, 1887, 2038 |
| Bst2UI | CCWGG | 3 cut(s) | 231, 940, 2253 |
| Bst4CI | ACNGT | 6 cut(s) | 432, 788, 1127, 1450, 1685, 1724 |
| Bst6I | CTCTTC | 2 cut(s) | 1281, 1491 |
| BstAFI | CTTAAG | 2 cut(s) | 253, 566 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 337, 842 |
| BstBAI | YACGTR | 2 cut(s) | 1796, 1919 |
| BstC8I | GCNNGC | 3 cut(s) | 150, 333, 1281 |
| BstDEI | CTNAG | 8 cut(s) | 64, 556, 1492, 1542, 1580, 1912, 1992, 2118 |
| BstDSI | CCRYGG | 1 cut(s) | 546 |
| BstEII | GGTNACC | 1 cut(s) | 903 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 984 |
| BstF5I | GGATG | 3 cut(s) | 348, 874, 1336 |
| BstFNI | CGCG | 1 cut(s) | 1656 |
| BstKTI | GATC | 9 cut(s) | 625, 769, 937, 998, 1736, 1762, 2173, 2290, 2304 |
| BstMAI | GTCTC | 5 cut(s) | 38, 156, 546, 1242, 1636 |
| BstMBI | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 337, 842, 851, 1777, 1814, 1823, 1982, 2123 |
| BstNI | CCWGG | 3 cut(s) | 231, 940, 2253 |
| BstPI | GGTNACC | 1 cut(s) | 903 |
| BstSCI | CCNGG | 5 cut(s) | 229, 938, 1419, 1420, 2251 |
| BstSFI | CTRYAG | 2 cut(s) | 1534, 2240 |
| BstSNI | TACGTA | 1 cut(s) | 1796 |
| BstUI | CGCG | 1 cut(s) | 1656 |
| BstV1I | GCAGC | 2 cut(s) | 493, 1341 |
| BstV2I | GAAGAC | 1 cut(s) | 1875 |
| BstX2I | RGATCY | 3 cut(s) | 622, 934, 2287 |
| BstYI | RGATCY | 3 cut(s) | 622, 934, 2287 |
| Bsu15I | ATCGAT | 2 cut(s) | 734, 2148 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1992 |
| BsuI | GTATCC | 5 cut(s) | 219, 818, 1075, 1275, 2202 |
| BsuRI | GGCC | 2 cut(s) | 1049, 2095 |
| BsuTUI | ATCGAT | 2 cut(s) | 734, 2148 |
| BtgI | CCRYGG | 1 cut(s) | 546 |
| BtsCI | GGATG | 3 cut(s) | 348, 874, 1336 |
| BtsI | GCAGTG | 2 cut(s) | 857, 2237 |
| BtsIMutI | CAGTG | 7 cut(s) | 381, 857, 1050, 1132, 2064, 2126, 2237 |
| Cac8I | GCNNGC | 3 cut(s) | 150, 333, 1281 |
| CciI | TCATGA | 2 cut(s) | 1840, 2140 |
| Cfr10I | RCCGGY | 1 cut(s) | 1858 |
| Cfr13I | GGNCC | 2 cut(s) | 668, 1011 |
| Cfr9I | CCCGGG | 1 cut(s) | 1420 |
| ClaI | ATCGAT | 2 cut(s) | 734, 2148 |
| CseI | GACGC | 1 cut(s) | 2079 |
| Csp6I | GTAC | 3 cut(s) | 1720, 1793, 1916 |
| CviAII | CATG | 6 cut(s) | 235, 292, 547, 1553, 1841, 2141 |
| CviQI | GTAC | 3 cut(s) | 1720, 1793, 1916 |
| DdeI | CTNAG | 8 cut(s) | 64, 556, 1492, 1542, 1580, 1912, 1992, 2118 |
| DpnI | GATC | 9 cut(s) | 624, 768, 936, 997, 1735, 1761, 2172, 2289, 2303 |
| DpnII | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| DraIII | CACNNNGTG | 1 cut(s) | 1607 |
| DriI | GACNNNNNGTC | 1 cut(s) | 511 |
| EaeI | YGGCCR | 1 cut(s) | 1047 |
| Eam1104I | CTCTTC | 2 cut(s) | 1281, 1491 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 511 |
| EarI | CTCTTC | 2 cut(s) | 1281, 1491 |
| EciI | GGCGGA | 1 cut(s) | 744 |
| Ecl136II | GAGCTC | 1 cut(s) | 1133 |
| Eco105I | TACGTA | 1 cut(s) | 1796 |
| Eco130I | CCWWGG | 3 cut(s) | 546, 1887, 2038 |
| Eco24I | GRGCYC | 1 cut(s) | 1135 |
| Eco31I | GGTCTC | 2 cut(s) | 38, 156 |
| Eco32I | GATATC | 2 cut(s) | 2224, 2271 |
| Eco47I | GGWCC | 2 cut(s) | 668, 1011 |
| Eco53kI | GAGCTC | 1 cut(s) | 1133 |
| Eco57I | CTGAAG | 5 cut(s) | 283, 969, 1026, 1305, 1454 |
| Eco81I | CCTNAGG | 1 cut(s) | 1992 |
| Eco88I | CYCGRG | 3 cut(s) | 664, 1206, 1420 |
| Eco91I | GGTNACC | 1 cut(s) | 903 |
| EcoICRI | GAGCTC | 1 cut(s) | 1133 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 984 |
| EcoO109I | RGGNCCY | 1 cut(s) | 668 |
| EcoO65I | GGTNACC | 1 cut(s) | 903 |
| EcoRI | GAATTC | 2 cut(s) | 1429, 1616 |
| EcoRII | CCWGG | 3 cut(s) | 229, 938, 2251 |
| EcoRV | GATATC | 2 cut(s) | 2224, 2271 |
| EcoT14I | CCWWGG | 3 cut(s) | 546, 1887, 2038 |
| EcoT38I | GRGCYC | 1 cut(s) | 1135 |
| ErhI | CCWWGG | 3 cut(s) | 546, 1887, 2038 |
| FaeI | CATG | 6 cut(s) | 238, 295, 550, 1556, 1844, 2144 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1999, 2031 |
| FaqI | GGGAC | 3 cut(s) | 334, 681, 2115 |
| FatI | CATG | 6 cut(s) | 234, 291, 546, 1552, 1840, 2140 |
| FauI | CCCGC | 3 cut(s) | 784, 1272, 1969 |
| FauNDI | CATATG | 1 cut(s) | 2298 |
| FblI | GTMKAC | 1 cut(s) | 1533 |
| Fnu4HI | GCNGC | 5 cut(s) | 482, 1352, 1355, 1407, 1986 |
| FokI | GGATG | 3 cut(s) | 335, 861, 1343 |
| FriOI | GRGCYC | 1 cut(s) | 1135 |
| Fsp4HI | GCNGC | 5 cut(s) | 482, 1352, 1355, 1407, 1986 |
| FspBI | CTAG | 3 cut(s) | 1031, 1628, 2114 |
| GluI | GCNGC | 5 cut(s) | 482, 1352, 1355, 1407, 1986 |
| GsaI | CCCAGC | 1 cut(s) | 836 |
| GsuI | CTGGAG | 3 cut(s) | 90, 545, 1266 |
| HaeIII | GGCC | 2 cut(s) | 1049, 2095 |
| HapII | CCGG | 4 cut(s) | 1068, 1294, 1421, 1859 |
| HgaI | GACGC | 1 cut(s) | 2079 |
| Hin1II | CATG | 6 cut(s) | 238, 295, 550, 1556, 1844, 2144 |
| HincII | GTYRAC | 2 cut(s) | 1853, 2157 |
| HindII | GTYRAC | 2 cut(s) | 1853, 2157 |
| HindIII | AAGCTT | 1 cut(s) | 1646 |
| HinfI | GANTC | 4 cut(s) | 200, 1023, 1966, 2128 |
| HpaII | CCGG | 4 cut(s) | 1068, 1294, 1421, 1859 |
| HphI | GGTGA | 7 cut(s) | 776, 879, 897, 969, 1447, 1582, 1859 |
| Hpy166II | GTNNAC | 6 cut(s) | 1014, 1534, 1853, 1867, 2157, 2237 |
| Hpy188I | TCNGA | 8 cut(s) | 353, 418, 427, 919, 1495, 1971, 2022, 2275 |
| Hpy8I | GTNNAC | 6 cut(s) | 1014, 1534, 1853, 1867, 2157, 2237 |
| Hpy99I | CGWCG | 1 cut(s) | 2030 |
| HpyAV | CCTTC | 6 cut(s) | 232, 351, 549, 921, 993, 1508 |
| HpyCH4III | ACNGT | 6 cut(s) | 432, 788, 1127, 1450, 1685, 1724 |
| HpyCH4IV | ACGT | 5 cut(s) | 1795, 1918, 2028, 2212, 2233 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 337, 842, 851, 1777, 1814, 1823, 1982, 2123 |
| HpyF3I | CTNAG | 8 cut(s) | 64, 556, 1492, 1542, 1580, 1912, 1992, 2118 |
| HpySE526I | ACGT | 5 cut(s) | 1795, 1918, 2028, 2212, 2233 |
| Hsp92II | CATG | 6 cut(s) | 238, 295, 550, 1556, 1844, 2144 |
| Kzo9I | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| Lsp1109I | GCAGC | 2 cut(s) | 493, 1341 |
| LweI | GCATC | 2 cut(s) | 1735, 2140 |
| MaeI | CTAG | 3 cut(s) | 1031, 1628, 2114 |
| MaeII | ACGT | 5 cut(s) | 1795, 1918, 2028, 2212, 2233 |
| MalI | GATC | 9 cut(s) | 624, 768, 936, 997, 1735, 1761, 2172, 2289, 2303 |
| MbiI | CCGCTC | 2 cut(s) | 1406, 1988 |
| MboI | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| MflI | RGATCY | 3 cut(s) | 622, 934, 2287 |
| MhlI | GDGCHC | 3 cut(s) | 130, 793, 1135 |
| MlsI | TGGCCA | 1 cut(s) | 1049 |
| MluCI | AATT | 8 cut(s) | 324, 693, 1004, 1053, 1429, 1486, 1616, 1873 |
| MluNI | TGGCCA | 1 cut(s) | 1049 |
| MlyI | GAGTC | 2 cut(s) | 194, 1975 |
| Mox20I | TGGCCA | 1 cut(s) | 1049 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1201 |
| MscI | TGGCCA | 1 cut(s) | 1049 |
| MseI | TTAA | 7 cut(s) | 102, 254, 567, 632, 1221, 1335, 1650 |
| Msp20I | TGGCCA | 1 cut(s) | 1049 |
| MspA1I | CMGCKG | 4 cut(s) | 68, 1279, 1354, 1932 |
| MspCI | CTTAAG | 2 cut(s) | 253, 566 |
| MspI | CCGG | 4 cut(s) | 1068, 1294, 1421, 1859 |
| MspR9I | CCNGG | 5 cut(s) | 231, 940, 1421, 1422, 2253 |
| MvaI | CCWGG | 3 cut(s) | 231, 940, 2253 |
| MvnI | CGCG | 1 cut(s) | 1656 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 337, 842, 851, 1777, 1814, 1823, 1982, 2123 |
| NciI | CCSGG | 2 cut(s) | 1421, 1422 |
| NcoI | CCATGG | 1 cut(s) | 546 |
| NdeI | CATATG | 1 cut(s) | 2298 |
| NdeII | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| NlaIII | CATG | 6 cut(s) | 238, 295, 550, 1556, 1844, 2144 |
| NlaIV | GGNNCC | 3 cut(s) | 669, 1212, 2250 |
| NmeAIII | GCCGAG | 2 cut(s) | 2113, 2121 |
| NmuCI | GTSAC | 4 cut(s) | 197, 903, 1607, 2208 |
| PagI | TCATGA | 2 cut(s) | 1840, 2140 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1201 |
| PfeI | GAWTC | 2 cut(s) | 1023, 2128 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 292 |
| PkrI | GCNGC | 5 cut(s) | 483, 1353, 1356, 1408, 1987 |
| PleI | GAGTC | 2 cut(s) | 194, 1974 |
| PpsI | GAGTC | 2 cut(s) | 194, 1974 |
| Ppu21I | YACGTR | 2 cut(s) | 1796, 1919 |
| PpuMI | RGGWCCY | 1 cut(s) | 668 |
| PshBI | ATTAAT | 1 cut(s) | 102 |
| PsiI | TTATAA | 2 cut(s) | 740, 1323 |
| Psp124BI | GAGCTC | 1 cut(s) | 1135 |
| Psp5II | RGGWCCY | 1 cut(s) | 668 |
| Psp6I | CCWGG | 3 cut(s) | 229, 938, 2251 |
| PspEI | GGTNACC | 1 cut(s) | 903 |
| PspFI | CCCAGC | 1 cut(s) | 832 |
| PspGI | CCWGG | 3 cut(s) | 229, 938, 2251 |
| PspN4I | GGNNCC | 3 cut(s) | 669, 1212, 2250 |
| PspPI | GGNCC | 2 cut(s) | 668, 1011 |
| PspPPI | RGGWCCY | 1 cut(s) | 668 |
| PstI | CTGCAG | 1 cut(s) | 2244 |
| PsuI | RGATCY | 3 cut(s) | 622, 934, 2287 |
| PvuII | CAGCTG | 1 cut(s) | 68 |
| RsaI | GTAC | 3 cut(s) | 1721, 1794, 1917 |
| RsaNI | GTAC | 3 cut(s) | 1720, 1793, 1916 |
| SacI | GAGCTC | 1 cut(s) | 1135 |
| SaqAI | TTAA | 7 cut(s) | 102, 254, 567, 632, 1221, 1335, 1650 |
| SatI | GCNGC | 5 cut(s) | 482, 1352, 1355, 1407, 1986 |
| Sau3AI | GATC | 9 cut(s) | 622, 766, 934, 995, 1733, 1759, 2170, 2287, 2301 |
| Sau96I | GGNCC | 2 cut(s) | 668, 1011 |
| ScaI | AGTACT | 1 cut(s) | 1721 |
| SchI | GAGTC | 2 cut(s) | 194, 1975 |
| ScrFI | CCNGG | 5 cut(s) | 231, 940, 1421, 1422, 2253 |
| SduI | GDGCHC | 3 cut(s) | 130, 793, 1135 |
| SfaNI | GCATC | 2 cut(s) | 1735, 2140 |
| SfcI | CTRYAG | 2 cut(s) | 1534, 2240 |
| SinI | GGWCC | 2 cut(s) | 668, 1011 |
| SmaI | CCCGGG | 1 cut(s) | 1422 |
| SmlI | CTYRAG | 3 cut(s) | 253, 367, 566 |
| SmoI | CTYRAG | 3 cut(s) | 253, 367, 566 |
| SnaBI | TACGTA | 1 cut(s) | 1796 |
| Sse9I | AATT | 8 cut(s) | 324, 693, 1004, 1053, 1429, 1486, 1616, 1873 |
| SspI | AATATT | 1 cut(s) | 1804 |
| SspMI | CTAG | 3 cut(s) | 1031, 1628, 2114 |
| SstI | GAGCTC | 1 cut(s) | 1135 |
| StyD4I | CCNGG | 5 cut(s) | 229, 938, 1419, 1420, 2251 |
| StyI | CCWWGG | 3 cut(s) | 546, 1887, 2038 |
| TaaI | ACNGT | 6 cut(s) | 432, 788, 1127, 1450, 1685, 1724 |
| TaiI | ACGT | 5 cut(s) | 1798, 1921, 2031, 2215, 2236 |
| TaqI | TCGA | 4 cut(s) | 734, 1230, 1520, 2148 |
| TasI | AATT | 8 cut(s) | 324, 693, 1004, 1053, 1429, 1486, 1616, 1873 |
| TatI | WGTACW | 1 cut(s) | 1719 |
| TauI | GCSGC | 3 cut(s) | 1354, 1409, 1988 |
| TfiI | GAWTC | 2 cut(s) | 1023, 2128 |
| Tru1I | TTAA | 7 cut(s) | 102, 254, 567, 632, 1221, 1335, 1650 |
| Tru9I | TTAA | 7 cut(s) | 102, 254, 567, 632, 1221, 1335, 1650 |
| TscAI | CASTG | 7 cut(s) | 388, 864, 1050, 1132, 2071, 2126, 2244 |
| TseFI | GTSAC | 4 cut(s) | 197, 903, 1607, 2208 |
| TseI | GCWGC | 2 cut(s) | 481, 1354 |
| Tsp45I | GTSAC | 4 cut(s) | 197, 903, 1607, 2208 |
| TspDTI | ATGAA | 6 cut(s) | 251, 267, 1305, 1829, 2129, 2213 |
| TspGWI | ACGGA | 3 cut(s) | 711, 2163, 2199 |
| TspMI | CCCGGG | 1 cut(s) | 1420 |
| TspRI | CASTG | 7 cut(s) | 388, 864, 1050, 1132, 2071, 2126, 2244 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 292 |
| Vha464I | CTTAAG | 2 cut(s) | 253, 566 |
| VpaK11BI | GGWCC | 2 cut(s) | 668, 1011 |
| VspI | ATTAAT | 1 cut(s) | 102 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 984 |
| XapI | RAATTY | 5 cut(s) | 693, 1004, 1429, 1616, 1873 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 897 |
| XmaI | CCCGGG | 1 cut(s) | 1420 |
| XmiI | GTMKAC | 1 cut(s) | 1533 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1201 |
| XspI | CTAG | 3 cut(s) | 1031, 1628, 2114 |
| ZrmI | AGTACT | 1 cut(s) | 1721 |