Belongs to the disease resistance NB-LRR family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1493 |
| Acc36I | ACCTGC | 1 cut(s) | 1493 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 925 |
| AccI | GTMKAC | 3 cut(s) | 187, 1831, 1871 |
| AccII | CGCG | 2 cut(s) | 1371, 2154 |
| AccIII | TCCGGA | 1 cut(s) | 823 |
| AciI | CCGC | 3 cut(s) | 142, 543, 1169 |
| AcuI | CTGAAG | 1 cut(s) | 2447 |
| AcyI | GRCGYC | 1 cut(s) | 1102 |
| AfaI | GTAC | 2 cut(s) | 533, 1515 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 925, 961, 1045, 1201 |
| AflII | CTTAAG | 1 cut(s) | 1196 |
| AflIII | ACRYGT | 1 cut(s) | 1114 |
| AjiI | CACGTC | 1 cut(s) | 1412 |
| AjnI | CCWGG | 2 cut(s) | 207, 1629 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 498, 530 |
| Alw21I | GWGCWC | 3 cut(s) | 132, 889, 2048 |
| Alw26I | GTCTC | 5 cut(s) | 52, 639, 1061, 1745, 2437 |
| Alw44I | GTGCAC | 1 cut(s) | 885 |
| Ama87I | CYCGRG | 2 cut(s) | 89, 1678 |
| Aor13HI | TCCGGA | 1 cut(s) | 823 |
| AoxI | GGCC | 3 cut(s) | 1036, 1446, 1485 |
| ApaLI | GTGCAC | 1 cut(s) | 885 |
| ApeKI | GCWGC | 3 cut(s) | 104, 1133, 2172 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 277, 309, 670, 702 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2237 |
| AspLEI | GCGC | 2 cut(s) | 1051, 1373 |
| AspS9I | GGNCC | 1 cut(s) | 870 |
| AsuC2I | CCSGG | 1 cut(s) | 993 |
| AsuHPI | GGTGA | 3 cut(s) | 398, 2372, 2549 |
| AsuNHI | GCTAGC | 1 cut(s) | 1708 |
| AvaI | CYCGRG | 2 cut(s) | 89, 1678 |
| AvaII | GGWCC | 1 cut(s) | 870 |
| AxyI | CCTNAGG | 1 cut(s) | 1824 |
| BaeGI | GKGCMC | 1 cut(s) | 889 |
| BamHI | GGATCC | 2 cut(s) | 826, 996 |
| BanII | GRGCYC | 2 cut(s) | 132, 2048 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1169, 1201 |
| BbsI | GAAGAC | 2 cut(s) | 246, 560 |
| Bbv12I | GWGCWC | 3 cut(s) | 132, 889, 2048 |
| BbvCI | CCTCAGC | 1 cut(s) | 1332 |
| BbvI | GCAGC | 3 cut(s) | 116, 1120, 2159 |
| BccI | CCATC | 7 cut(s) | 503, 1301, 1378, 1391, 1397, 1561, 2188 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 880, 914 |
| BciT130I | CCWGG | 2 cut(s) | 209, 1631 |
| BciVI | GTATCC | 1 cut(s) | 2110 |
| BcnI | CCSGG | 1 cut(s) | 993 |
| BcoDI | GTCTC | 5 cut(s) | 52, 639, 1061, 1745, 2437 |
| BfaI | CTAG | 9 cut(s) | 59, 180, 1709, 1772, 1944, 2078, 2120, 2324, 2465 |
| BfmI | CTRYAG | 2 cut(s) | 1134, 2173 |
| BfoI | RGCGCY | 1 cut(s) | 1052 |
| BfrI | CTTAAG | 1 cut(s) | 1196 |
| BfuAI | ACCTGC | 1 cut(s) | 1493 |
| BfuI | GTATCC | 1 cut(s) | 2110 |
| BglII | AGATCT | 3 cut(s) | 2082, 2294, 2433 |
| BisI | GCNGC | 3 cut(s) | 105, 1134, 2173 |
| BlpI | GCTNAGC | 1 cut(s) | 138 |
| BlsI | GCNGC | 3 cut(s) | 106, 1135, 2174 |
| Bme1390I | CCNGG | 3 cut(s) | 209, 993, 1631 |
| Bme18I | GGWCC | 1 cut(s) | 870 |
| BmeT110I | CYCGRG | 2 cut(s) | 89, 1678 |
| BmgBI | CACGTC | 1 cut(s) | 1412 |
| BmgT120I | GGNCC | 1 cut(s) | 870 |
| BmiI | GGNNCC | 3 cut(s) | 828, 872, 998 |
| BmrFI | CCNGG | 3 cut(s) | 209, 993, 1631 |
| BmrI | ACTGGG | 2 cut(s) | 1478, 1905 |
| BmsI | GCATC | 4 cut(s) | 1240, 1382, 2537, 2559 |
| BmtI | GCTAGC | 1 cut(s) | 1712 |
| BmuI | ACTGGG | 2 cut(s) | 1478, 1905 |
| BpiI | GAAGAC | 2 cut(s) | 246, 560 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1332 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 138 |
| BpuEI | CTTGAG | 7 cut(s) | 116, 376, 621, 772, 1904, 2236, 2275 |
| BpuMI | CCSGG | 1 cut(s) | 993 |
| Bsa29I | ATCGAT | 1 cut(s) | 2019 |
| BsaAI | YACGTR | 2 cut(s) | 1517, 2558 |
| BsaHI | GRCGYC | 1 cut(s) | 1102 |
| BsaI | GGTCTC | 2 cut(s) | 1061, 2437 |
| BsaJI | CCNNGG | 3 cut(s) | 992, 1630, 1952 |
| BsaWI | WCCGGW | 1 cut(s) | 823 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 319, 349 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 925, 961, 1045, 1201 |
| Bse118I | RCCGGY | 1 cut(s) | 300 |
| Bse1I | ACTGG | 2 cut(s) | 1473, 1900 |
| Bse21I | CCTNAGG | 1 cut(s) | 1824 |
| BseAI | TCCGGA | 1 cut(s) | 823 |
| BseBI | CCWGG | 2 cut(s) | 209, 1631 |
| BseCI | ATCGAT | 1 cut(s) | 2019 |
| BseDI | CCNNGG | 3 cut(s) | 992, 1630, 1952 |
| BseGI | GGATG | 6 cut(s) | 151, 904, 1399, 1408, 1563, 2552 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 925, 961, 1045, 1201 |
| BseMII | CTCAG | 7 cut(s) | 584, 801, 1323, 1797, 2051, 2175, 2489 |
| BseNI | ACTGG | 2 cut(s) | 1473, 1900 |
| BseRI | GAGGAG | 2 cut(s) | 188, 340 |
| BseSI | GKGCMC | 1 cut(s) | 889 |
| BseXI | GCAGC | 3 cut(s) | 116, 1120, 2159 |
| BsgI | GTGCAG | 1 cut(s) | 1521 |
| Bsh1236I | CGCG | 2 cut(s) | 1371, 2154 |
| BshFI | GGCC | 3 cut(s) | 1038, 1448, 1487 |
| BshVI | ATCGAT | 1 cut(s) | 2019 |
| BsiHKAI | GWGCWC | 3 cut(s) | 132, 889, 2048 |
| BsiHKCI | CYCGRG | 2 cut(s) | 89, 1678 |
| BsiSI | CCGG | 4 cut(s) | 301, 824, 992, 1754 |
| BsiWI | CGTACG | 1 cut(s) | 1513 |
| BslFI | GGGAC | 1 cut(s) | 856 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 925, 961, 1045, 1201 |
| BsmAI | GTCTC | 5 cut(s) | 52, 639, 1061, 1745, 2437 |
| BsmFI | GGGAC | 1 cut(s) | 856 |
| BsnI | GGCC | 3 cut(s) | 1038, 1448, 1487 |
| Bso31I | GGTCTC | 2 cut(s) | 1061, 2437 |
| BsoBI | CYCGRG | 2 cut(s) | 89, 1678 |
| Bsp1286I | GDGCHC | 3 cut(s) | 132, 889, 2048 |
| Bsp13I | TCCGGA | 1 cut(s) | 823 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 138 |
| Bsp19I | CCATGG | 1 cut(s) | 1952 |
| Bsp68I | TCGCGA | 1 cut(s) | 2154 |
| BspACI | CCGC | 3 cut(s) | 142, 543, 1169 |
| BspANI | GGCC | 3 cut(s) | 1038, 1448, 1487 |
| BspCNI | CTCAG | 7 cut(s) | 583, 802, 1324, 1798, 2052, 2176, 2490 |
| BspDI | ATCGAT | 1 cut(s) | 2019 |
| BspEI | TCCGGA | 1 cut(s) | 823 |
| BspFNI | CGCG | 2 cut(s) | 1371, 2154 |
| BspLI | GGNNCC | 3 cut(s) | 828, 872, 998 |
| BspMAI | CTGCAG | 2 cut(s) | 1138, 2177 |
| BspMI | ACCTGC | 1 cut(s) | 1493 |
| BspOI | GCTAGC | 1 cut(s) | 1712 |
| BspTI | CTTAAG | 1 cut(s) | 1196 |
| BspTNI | GGTCTC | 2 cut(s) | 1061, 2437 |
| BsrFI | RCCGGY | 1 cut(s) | 300 |
| BsrI | ACTGG | 2 cut(s) | 1473, 1900 |
| BssAI | RCCGGY | 1 cut(s) | 300 |
| BssECI | CCNNGG | 3 cut(s) | 992, 1630, 1952 |
| BssNAI | GTATAC | 2 cut(s) | 1832, 1872 |
| BssNI | GRCGYC | 1 cut(s) | 1102 |
| BssT1I | CCWWGG | 1 cut(s) | 1952 |
| Bst1107I | GTATAC | 2 cut(s) | 1832, 1872 |
| Bst2UI | CCWGG | 2 cut(s) | 209, 1631 |
| Bst4CI | ACNGT | 3 cut(s) | 235, 536, 1969 |
| Bst6I | CTCTTC | 4 cut(s) | 261, 450, 924, 2337 |
| BstACI | GRCGYC | 1 cut(s) | 1102 |
| BstAFI | CTTAAG | 1 cut(s) | 1196 |
| BstBAI | YACGTR | 2 cut(s) | 1517, 2558 |
| BstC8I | GCNNGC | 7 cut(s) | 414, 889, 1500, 1700, 1710, 2313, 2531 |
| BstDSI | CCRYGG | 1 cut(s) | 1952 |
| BstF5I | GGATG | 6 cut(s) | 151, 904, 1399, 1408, 1563, 2552 |
| BstFNI | CGCG | 2 cut(s) | 1371, 2154 |
| BstH2I | RGCGCY | 1 cut(s) | 1052 |
| BstHHI | GCGC | 2 cut(s) | 1051, 1373 |
| BstMAI | GTCTC | 5 cut(s) | 52, 639, 1061, 1745, 2437 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 306, 2603 |
| BstNI | CCWGG | 2 cut(s) | 209, 1631 |
| BstNSI | RCATGY | 3 cut(s) | 1118, 1702, 2315 |
| BstSCI | CCNGG | 3 cut(s) | 207, 991, 1629 |
| BstSFI | CTRYAG | 2 cut(s) | 1134, 2173 |
| BstSLI | GKGCMC | 1 cut(s) | 889 |
| BstSNI | TACGTA | 1 cut(s) | 2558 |
| BstUI | CGCG | 2 cut(s) | 1371, 2154 |
| BstV1I | GCAGC | 3 cut(s) | 116, 1120, 2159 |
| BstV2I | GAAGAC | 2 cut(s) | 246, 560 |
| BstX2I | RGATCY | 8 cut(s) | 826, 996, 1720, 1957, 2082, 2090, 2294, 2433 |
| BstYI | RGATCY | 8 cut(s) | 826, 996, 1720, 1957, 2082, 2090, 2294, 2433 |
| BstZ17I | GTATAC | 2 cut(s) | 1832, 1872 |
| Bsu15I | ATCGAT | 1 cut(s) | 2019 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1824 |
| BsuI | GTATCC | 1 cut(s) | 2110 |
| BsuRI | GGCC | 3 cut(s) | 1038, 1448, 1487 |
| BsuTUI | ATCGAT | 1 cut(s) | 2019 |
| BtgI | CCRYGG | 1 cut(s) | 1952 |
| BtrI | CACGTC | 1 cut(s) | 1412 |
| BtsCI | GGATG | 6 cut(s) | 151, 904, 1399, 1408, 1563, 2552 |
| BtsIMutI | CAGTG | 1 cut(s) | 1974 |
| BtuMI | TCGCGA | 1 cut(s) | 2154 |
| BveI | ACCTGC | 1 cut(s) | 1493 |
| Cac8I | GCNNGC | 7 cut(s) | 414, 889, 1500, 1700, 1710, 2313, 2531 |
| CfoI | GCGC | 2 cut(s) | 1051, 1373 |
| Cfr10I | RCCGGY | 1 cut(s) | 300 |
| Cfr13I | GGNCC | 1 cut(s) | 870 |
| ClaI | ATCGAT | 1 cut(s) | 2019 |
| CseI | GACGC | 1 cut(s) | 1091 |
| Csp6I | GTAC | 2 cut(s) | 532, 1514 |
| CviQI | GTAC | 2 cut(s) | 532, 1514 |
| Eam1104I | CTCTTC | 4 cut(s) | 261, 450, 924, 2337 |
| EarI | CTCTTC | 4 cut(s) | 261, 450, 924, 2337 |
| Ecl136II | GAGCTC | 2 cut(s) | 130, 2046 |
| Eco105I | TACGTA | 1 cut(s) | 2558 |
| Eco130I | CCWWGG | 1 cut(s) | 1952 |
| Eco147I | AGGCCT | 1 cut(s) | 1487 |
| Eco24I | GRGCYC | 2 cut(s) | 132, 2048 |
| Eco31I | GGTCTC | 2 cut(s) | 1061, 2437 |
| Eco32I | GATATC | 1 cut(s) | 1902 |
| Eco47I | GGWCC | 1 cut(s) | 870 |
| Eco53kI | GAGCTC | 2 cut(s) | 130, 2046 |
| Eco57I | CTGAAG | 1 cut(s) | 2447 |
| Eco81I | CCTNAGG | 1 cut(s) | 1824 |
| Eco88I | CYCGRG | 2 cut(s) | 89, 1678 |
| EcoICRI | GAGCTC | 2 cut(s) | 130, 2046 |
| EcoRI | GAATTC | 3 cut(s) | 203, 1123, 1685 |
| EcoRII | CCWGG | 2 cut(s) | 207, 1629 |
| EcoRV | GATATC | 1 cut(s) | 1902 |
| EcoT14I | CCWWGG | 1 cut(s) | 1952 |
| EcoT22I | ATGCAT | 2 cut(s) | 1428, 2552 |
| EcoT38I | GRGCYC | 2 cut(s) | 132, 2048 |
| ErhI | CCWWGG | 1 cut(s) | 1952 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1472, 1504 |
| FaqI | GGGAC | 1 cut(s) | 856 |
| FauI | CCCGC | 1 cut(s) | 135 |
| FauNDI | CATATG | 1 cut(s) | 788 |
| FblI | GTMKAC | 3 cut(s) | 187, 1831, 1871 |
| Fnu4HI | GCNGC | 3 cut(s) | 105, 1134, 2173 |
| FokI | GGATG | 6 cut(s) | 158, 891, 1406, 1415, 1570, 2559 |
| FriOI | GRGCYC | 2 cut(s) | 132, 2048 |
| Fsp4HI | GCNGC | 3 cut(s) | 105, 1134, 2173 |
| FspBI | CTAG | 9 cut(s) | 59, 180, 1709, 1772, 1944, 2078, 2120, 2324, 2465 |
| GlaI | GCGC | 2 cut(s) | 1050, 1372 |
| GluI | GCNGC | 3 cut(s) | 105, 1134, 2173 |
| HaeII | RGCGCY | 1 cut(s) | 1052 |
| HaeIII | GGCC | 3 cut(s) | 1038, 1448, 1487 |
| HapII | CCGG | 4 cut(s) | 301, 824, 992, 1754 |
| HgaI | GACGC | 1 cut(s) | 1091 |
| HhaI | GCGC | 2 cut(s) | 1051, 1373 |
| Hin1I | GRCGYC | 1 cut(s) | 1102 |
| Hin6I | GCGC | 2 cut(s) | 1049, 1371 |
| HinP1I | GCGC | 2 cut(s) | 1049, 1371 |
| HindIII | AAGCTT | 6 cut(s) | 414, 680, 1880, 2147, 2349, 2504 |
| HpaII | CCGG | 4 cut(s) | 301, 824, 992, 1754 |
| HphI | GGTGA | 3 cut(s) | 398, 2372, 2549 |
| Hpy166II | GTNNAC | 5 cut(s) | 188, 705, 887, 1832, 1872 |
| Hpy188I | TCNGA | 9 cut(s) | 202, 818, 1263, 1465, 1659, 1929, 2185, 2274, 2499 |
| Hpy8I | GTNNAC | 5 cut(s) | 188, 705, 887, 1832, 1872 |
| Hpy99I | CGWCG | 2 cut(s) | 1413, 2228 |
| HpyCH4III | ACNGT | 3 cut(s) | 235, 536, 1969 |
| HpyCH4IV | ACGT | 8 cut(s) | 847, 1056, 1411, 1431, 1516, 2267, 2557, 2572 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 306, 2603 |
| HpySE526I | ACGT | 8 cut(s) | 847, 1056, 1411, 1431, 1516, 2267, 2557, 2572 |
| Hsp92I | GRCGYC | 1 cut(s) | 1102 |
| HspAI | GCGC | 2 cut(s) | 1049, 1371 |
| Kpn2I | TCCGGA | 1 cut(s) | 823 |
| LmnI | GCTCC | 6 cut(s) | 327, 1204, 1632, 1975, 2051, 2287 |
| Lsp1109I | GCAGC | 3 cut(s) | 116, 1120, 2159 |
| LweI | GCATC | 4 cut(s) | 1240, 1382, 2537, 2559 |
| MaeI | CTAG | 9 cut(s) | 59, 180, 1709, 1772, 1944, 2078, 2120, 2324, 2465 |
| MaeII | ACGT | 8 cut(s) | 847, 1056, 1411, 1431, 1516, 2267, 2557, 2572 |
| MaeIII | GTNAC | 2 cut(s) | 1103, 2558 |
| MfeI | CAATTG | 3 cut(s) | 1693, 2242, 2636 |
| MflI | RGATCY | 8 cut(s) | 826, 996, 1720, 1957, 2082, 2090, 2294, 2433 |
| MhlI | GDGCHC | 3 cut(s) | 132, 889, 2048 |
| MlyI | GAGTC | 3 cut(s) | 859, 1669, 2071 |
| MmeI | TCCRAC | 1 cut(s) | 946 |
| Mph1103I | ATGCAT | 2 cut(s) | 1428, 2552 |
| MroI | TCCGGA | 1 cut(s) | 823 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2237 |
| MseI | TTAA | 8 cut(s) | 320, 1197, 1533, 1667, 2006, 2166, 2190, 2649 |
| MspCI | CTTAAG | 1 cut(s) | 1196 |
| MspI | CCGG | 4 cut(s) | 301, 824, 992, 1754 |
| MspR9I | CCNGG | 3 cut(s) | 209, 993, 1631 |
| MunI | CAATTG | 3 cut(s) | 1693, 2242, 2636 |
| MvaI | CCWGG | 2 cut(s) | 209, 1631 |
| MvnI | CGCG | 2 cut(s) | 1371, 2154 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 306, 2603 |
| NciI | CCSGG | 1 cut(s) | 993 |
| NcoI | CCATGG | 1 cut(s) | 1952 |
| NdeI | CATATG | 1 cut(s) | 788 |
| NheI | GCTAGC | 1 cut(s) | 1708 |
| NlaIV | GGNNCC | 3 cut(s) | 828, 872, 998 |
| NmuCI | GTSAC | 1 cut(s) | 1103 |
| NruI | TCGCGA | 1 cut(s) | 2154 |
| NsiI | ATGCAT | 2 cut(s) | 1428, 2552 |
| NspI | RCATGY | 3 cut(s) | 1118, 1702, 2315 |
| PaeI | GCATGC | 2 cut(s) | 1702, 2315 |
| PaeR7I | CTCGAG | 2 cut(s) | 89, 1678 |
| PaqCI | CACCTGC | 1 cut(s) | 1493 |
| PceI | AGGCCT | 1 cut(s) | 1487 |
| PciI | ACATGT | 1 cut(s) | 1114 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1062 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2237 |
| PfeI | GAWTC | 7 cut(s) | 97, 1579, 2123, 2366, 2374, 2516, 2567 |
| Pfl23II | CGTACG | 1 cut(s) | 1513 |
| PflFI | GACNNNGTC | 2 cut(s) | 868, 1072 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 925 |
| PkrI | GCNGC | 3 cut(s) | 106, 1135, 2174 |
| PleI | GAGTC | 3 cut(s) | 859, 1668, 2070 |
| PpsI | GAGTC | 3 cut(s) | 859, 1668, 2070 |
| Ppu21I | YACGTR | 2 cut(s) | 1517, 2558 |
| PscI | ACATGT | 1 cut(s) | 1114 |
| Psp124BI | GAGCTC | 2 cut(s) | 132, 2048 |
| Psp6I | CCWGG | 2 cut(s) | 207, 1629 |
| PspGI | CCWGG | 2 cut(s) | 207, 1629 |
| PspLI | CGTACG | 1 cut(s) | 1513 |
| PspN4I | GGNNCC | 3 cut(s) | 828, 872, 998 |
| PspPI | GGNCC | 1 cut(s) | 870 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1458, 1490 |
| PstI | CTGCAG | 2 cut(s) | 1138, 2177 |
| PsuI | RGATCY | 8 cut(s) | 826, 996, 1720, 1957, 2082, 2090, 2294, 2433 |
| PsyI | GACNNNGTC | 2 cut(s) | 868, 1072 |
| RruI | TCGCGA | 1 cut(s) | 2154 |
| RsaI | GTAC | 2 cut(s) | 533, 1515 |
| RsaNI | GTAC | 2 cut(s) | 532, 1514 |
| SacI | GAGCTC | 2 cut(s) | 132, 2048 |
| SaqAI | TTAA | 8 cut(s) | 320, 1197, 1533, 1667, 2006, 2166, 2190, 2649 |
| SatI | GCNGC | 3 cut(s) | 105, 1134, 2173 |
| Sau96I | GGNCC | 1 cut(s) | 870 |
| SchI | GAGTC | 3 cut(s) | 859, 1669, 2071 |
| ScrFI | CCNGG | 3 cut(s) | 209, 993, 1631 |
| SduI | GDGCHC | 3 cut(s) | 132, 889, 2048 |
| SfaNI | GCATC | 4 cut(s) | 1240, 1382, 2537, 2559 |
| SfcI | CTRYAG | 2 cut(s) | 1134, 2173 |
| Sfr274I | CTCGAG | 2 cut(s) | 89, 1678 |
| SinI | GGWCC | 1 cut(s) | 870 |
| SlaI | CTCGAG | 2 cut(s) | 89, 1678 |
| SnaBI | TACGTA | 1 cut(s) | 2558 |
| SphI | GCATGC | 2 cut(s) | 1702, 2315 |
| SseBI | AGGCCT | 1 cut(s) | 1487 |
| SsiI | CCGC | 3 cut(s) | 142, 543, 1169 |
| SspMI | CTAG | 9 cut(s) | 59, 180, 1709, 1772, 1944, 2078, 2120, 2324, 2465 |
| SstI | GAGCTC | 2 cut(s) | 132, 2048 |
| StuI | AGGCCT | 1 cut(s) | 1487 |
| StyD4I | CCNGG | 3 cut(s) | 207, 991, 1629 |
| StyI | CCWWGG | 1 cut(s) | 1952 |
| TaaI | ACNGT | 3 cut(s) | 235, 536, 1969 |
| TaiI | ACGT | 8 cut(s) | 850, 1059, 1414, 1434, 1519, 2270, 2560, 2575 |
| TaqII | GACCGA | 1 cut(s) | 858 |
| TfiI | GAWTC | 7 cut(s) | 97, 1579, 2123, 2366, 2374, 2516, 2567 |
| Tru1I | TTAA | 8 cut(s) | 320, 1197, 1533, 1667, 2006, 2166, 2190, 2649 |
| Tru9I | TTAA | 8 cut(s) | 320, 1197, 1533, 1667, 2006, 2166, 2190, 2649 |
| TscAI | CASTG | 1 cut(s) | 1974 |
| TseFI | GTSAC | 1 cut(s) | 1103 |
| TseI | GCWGC | 3 cut(s) | 104, 1133, 2172 |
| Tsp45I | GTSAC | 1 cut(s) | 1103 |
| TspDTI | ATGAA | 9 cut(s) | 80, 137, 635, 681, 1116, 1480, 1571, 1617, 2619 |
| TspGWI | ACGGA | 2 cut(s) | 21, 2156 |
| TspRI | CASTG | 1 cut(s) | 1974 |
| Tth111I | GACNNNGTC | 2 cut(s) | 868, 1072 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 925 |
| Vha464I | CTTAAG | 1 cut(s) | 1196 |
| VneI | GTGCAC | 1 cut(s) | 885 |
| VpaK11BI | GGWCC | 1 cut(s) | 870 |
| XbaI | TCTAGA | 3 cut(s) | 1943, 2119, 2464 |
| XceI | RCATGY | 3 cut(s) | 1118, 1702, 2315 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 962 |
| XhoI | CTCGAG | 2 cut(s) | 89, 1678 |
| XmiI | GTMKAC | 3 cut(s) | 187, 1831, 1871 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2237 |
| XspI | CTAG | 9 cut(s) | 59, 180, 1709, 1772, 1944, 2078, 2120, 2324, 2465 |
| Zsp2I | ATGCAT | 2 cut(s) | 1428, 2552 |