AT1G64105

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0022108
Physical Location & Seq
Forward (+)
23795050 .. 23795642
593 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G64105.1

Sequence Viewer

Length: 486 bp
ATGCAGGCGGAGGAGATAATATGCCGTGTTTCAGACGAAGAGATCATCGAGAATTACCTGAGGCCAAAGATAAACGGTGAAACATCTTCAATCCCGAGATATGTTGTGGAATTGGCAGAGGAGCTGTACACTGTAGAGCCTTGGCTGCTTCCTCGACAAACAGCTCCAATTCTCAACCCTGGCGAGTGGTTCTACTTTGGGAAACGCAATCGAAAGTATAGTAACTTGGAAGGTGTTCATTGCGAGGGATCGTGGATACTCGAAGACGGTTGCATCGCAGTCCTGTCCAAGGAGACGGGTGAGGAAATTGGTGGAACGACGAGATTTAGGTATTACTATAGGAACAAGGGGGATAAAGAGAGTCGTCTGAAGATGAGCAATTGGTTTATGAGAGAGTATCGTCTCTATTACAAATCCAGGAGAGTCTTCAATGGACGTCAGGTTTTCTGCATTATCACTTGTAATGATGAACATTTTATCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM PPR_2 RWP-RK

Protein Analysis

161

Amino Acids

19.22

Weight (kDa)

5.86

Isoelectric Point (pI)

46.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 11 - 135 1.3e-16 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022108)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G64100 AT1G64100 AT1G64100 AT1G64105

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 439
AciI CCGC 1 cut(s) 8
AclWI GGATC 1 cut(s) 256
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 436
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 289
AgsI TTSAA 2 cut(s) 90, 430
AjnI CCWGG 2 cut(s) 178, 416
AluBI AGCT 2 cut(s) 124, 164
AluI AGCT 2 cut(s) 124, 164
Alw26I GTCTC 2 cut(s) 287, 407
AlwI GGATC 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 94
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 1 cut(s) 145
Asp700I GAANNNNTTC 1 cut(s) 234
AsuHPI GGTGA 2 cut(s) 89, 311
AvaI CYCGRG 1 cut(s) 94
AxyI CCTNAGG 1 cut(s) 59
BbsI GAAGAC 2 cut(s) 270, 418
BbvI GCAGC 1 cut(s) 132
BceAI ACGGC 1 cut(s) 9
BciT130I CCWGG 2 cut(s) 180, 418
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 2 cut(s) 287, 407
BfmI CTRYAG 2 cut(s) 132, 337
BfuI GTATCC 1 cut(s) 249
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 2 cut(s) 180, 418
BmeT110I CYCGRG 1 cut(s) 94
BmrFI CCNGG 2 cut(s) 180, 418
BmsI GCATC 1 cut(s) 282
BpiI GAAGAC 2 cut(s) 270, 418
BsaHI GRCGYC 1 cut(s) 436
BsaJI CCNNGG 3 cut(s) 140, 178, 288
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse21I CCTNAGG 1 cut(s) 59
Bse3DI GCAATG 1 cut(s) 238
BseBI CCWGG 2 cut(s) 180, 418
BseDI CCNNGG 3 cut(s) 140, 178, 288
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 1 cut(s) 50
BseRI GAGGAG 2 cut(s) 26, 134
BseXI GCAGC 1 cut(s) 132
BshFI GGCC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 94
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 2 cut(s) 287, 407
BsmBI CGTCTC 2 cut(s) 287, 407
BsnI GGCC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 94
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 2 cut(s) 42, 248
BspACI CCGC 1 cut(s) 8
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 51
BspPI GGATC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 238
BsrGI TGTACA 1 cut(s) 126
BssECI CCNNGG 3 cut(s) 140, 178, 288
BssMI GATC 2 cut(s) 42, 248
BssNI GRCGYC 1 cut(s) 436
BssT1I CCWWGG 2 cut(s) 140, 288
Bst2UI CCWGG 2 cut(s) 180, 418
Bst4CI ACNGT 3 cut(s) 77, 133, 269
Bst6I CTCTTC 1 cut(s) 33
BstACI GRCGYC 1 cut(s) 436
BstAUI TGTACA 1 cut(s) 126
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 59
BstENI CCTNNNNNAGG 1 cut(s) 287
BstKTI GATC 2 cut(s) 45, 251
BstMAI GTCTC 2 cut(s) 287, 407
BstMBI GATC 2 cut(s) 42, 248
BstMWI GCNNNNNNNGC 1 cut(s) 145
BstNI CCWGG 2 cut(s) 180, 418
BstSCI CCNGG 2 cut(s) 178, 416
BstSFI CTRYAG 2 cut(s) 132, 337
BstV1I GCAGC 1 cut(s) 132
BstV2I GAAGAC 2 cut(s) 270, 418
Bsu36I CCTNAGG 1 cut(s) 59
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 259
BtsIMutI CAGTG 1 cut(s) 129
Cac8I GCNNGC 1 cut(s) 6
Csp6I GTAC 1 cut(s) 127
CviJI RGCY 5 cut(s) 64, 124, 139, 145, 164
CviKI_1 RGCY 5 cut(s) 64, 124, 139, 145, 164
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 1 cut(s) 59
DpnI GATC 2 cut(s) 44, 250
DpnII GATC 2 cut(s) 42, 248
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 2 cut(s) 140, 288
Eco57I CTGAAG 1 cut(s) 389
Eco81I CCTNAGG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 94
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 178, 416
EcoT14I CCWWGG 2 cut(s) 140, 288
ErhI CCWWGG 2 cut(s) 140, 288
Esp3I CGTCTC 2 cut(s) 287, 407
FaiI YATR 5 cut(s) 22, 102, 219, 339, 389
Fnu4HI GCNGC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 146
HaeIII GGCC 1 cut(s) 64
Hin1I GRCGYC 1 cut(s) 436
HinfI GANTC 2 cut(s) 361, 423
HphI GGTGA 2 cut(s) 89, 311
Hpy166II GTNNAC 1 cut(s) 129
Hpy188I TCNGA 2 cut(s) 34, 369
Hpy188III TCNNGA 2 cut(s) 49, 94
Hpy8I GTNNAC 1 cut(s) 129
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 224
HpyCH4III ACNGT 3 cut(s) 77, 133, 269
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 3 cut(s) 4, 273, 450
HpyF10VI GCNNNNNNNGC 1 cut(s) 145
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 436
Hsp92I GRCGYC 1 cut(s) 436
Kzo9I GATC 2 cut(s) 42, 248
LmnI GCTCC 2 cut(s) 121, 169
LpnPI CCDG 7 cut(s) 71, 165, 192, 296, 403, 425, 430
Lsp1109I GCAGC 1 cut(s) 132
LweI GCATC 1 cut(s) 282
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 221
MalI GATC 2 cut(s) 44, 250
MboI GATC 2 cut(s) 42, 248
MboII GAAGA 5 cut(s) 50, 78, 275, 382, 418
MfeI CAATTG 1 cut(s) 379
MluCI AATT 5 cut(s) 52, 110, 168, 306, 379
MlyI GAGTC 2 cut(s) 370, 432
MnlI CCTC 6 cut(s) 4, 54, 112, 162, 238, 295
MroXI GAANNNNTTC 1 cut(s) 234
MspR9I CCNGG 2 cut(s) 180, 418
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 2 cut(s) 180, 418
MwoI GCNNNNNNNGC 1 cut(s) 145
NdeII GATC 2 cut(s) 42, 248
PdmI GAANNNNTTC 1 cut(s) 234
PfoI TCCNGGA 1 cut(s) 416
PkrI GCNGC 1 cut(s) 147
PleI GAGTC 2 cut(s) 369, 431
PpsI GAGTC 2 cut(s) 369, 431
Psp6I CCWGG 2 cut(s) 178, 416
PspGI CCWGG 2 cut(s) 178, 416
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 2 cut(s) 42, 248
SchI GAGTC 2 cut(s) 370, 432
ScrFI CCNGG 2 cut(s) 180, 418
SetI ASST 7 cut(s) 60, 126, 166, 235, 332, 439, 444
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 2 cut(s) 132, 337
Sse9I AATT 5 cut(s) 52, 110, 168, 306, 379
SsiI CCGC 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 178, 416
StyI CCWWGG 2 cut(s) 140, 288
TaaI ACNGT 3 cut(s) 77, 133, 269
TaiI ACGT 1 cut(s) 439
TaqI TCGA 5 cut(s) 48, 154, 211, 261, 480
TasI AATT 5 cut(s) 52, 110, 168, 306, 379
TatI WGTACW 1 cut(s) 126
TscAI CASTG 1 cut(s) 136
TseI GCWGC 1 cut(s) 145
TspDTI ATGAA 2 cut(s) 227, 483
TspRI CASTG 1 cut(s) 136
XagI CCTNNNNNAGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 234
ZraI GACGTC 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.