AT1G74650
MYB Family

myb domain protein 31

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0023956
Physical Location & Seq
Forward (+)
28041146 .. 28043022
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G74650.1

Sequence Viewer

Length: 993 bp
ATGGGTAGACCACCTTGTTGCGAGAAGATTGAGGTGAAGAAAGGACCATGGACTCCCGAAGAAGACATAATCTTGGTCTCTTATATCCAACAACACGGCCCTGGAAATTGGAGATCTGTCCCTGCAAACACCGGTTTGCTAAGGTGTAGCAAGAGTTGCAGACTTAGATGGACTAATTACCTTCGTCCCGGGATCAAACGAGGAAATTTCACTCAACCGGAAGAGAAGATGATCATCCACCTTCAAGCTCTTTTGGGAAATAGATGGGCAGCTATAGCATCATATCTACCTCAGAGGACCGACAATGATATCAAGAACTACTGGAACACTCATCTTAAAAAGAAACTAGTGATGATGAAGTTTCAAAATGGTATCATCAACGAAAACAAAACCAATCTGGCAACAGATATTTCGTCTTGTAATAATAACAACAATGGATGTAATCACAACAAAAGGACCACCAACAAAGGCCAATGGGAGAAAAAACTTCAAACAGACATCAACATGGCCAAACAAGCCTTATTCCAAGCCTTGTCACTTGACCAACCATCTTCATTGATCCCTCCCGATCCTGACTCACCAAAACCTCATCATCATTCTACCACCACTTATGCCTCAAGCACAGATAACATCTCTAAATTACTCCAGAACTGGACAAGCTCATCATCGTCAAAGCCTAACACTTCATCAGTCTCCAACAACCGGAGCTCAAGCCCCGGTGAAGGAGGACTTTTTGATCATCACTCTTTGTTCTCATCGAATTCAGAATCTGGATCAGTTGATGAGAAGCTGAATTTGATGTCCGAGACAAGCATGTTCAAAGGTGAGAGCAAGCCAGACATAGACATGGAAGCTACACCTACTACTACTACTACTGATGATCAAGGCTCGTTGTCATTGATCGAGAAATGGTTGTTTGATGATCAAGGCTTGGTTCAGTGTGATGATAGTCAAGAAGATCTCATCGACGTGTCTTTAGAGGAGTTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001101 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009723 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009787 GO:0009889 GO:0009891 GO:0009893 GO:0009966 GO:0009987 GO:0010025 GO:0010033 GO:0010035 GO:0010038 GO:0010115 GO:0010166 GO:0010468 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0014070 GO:0019216 GO:0019219 GO:0019222 GO:0019747 GO:0023051 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032502 GO:0033993 GO:0042221 GO:0042493 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043455 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0046677 GO:0046686 GO:0046890 GO:0048518 GO:0048522 GO:0048580 GO:0048583 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051239 GO:0051252 GO:0051254 GO:0060255 GO:0062012 GO:0065007 GO:0071704 GO:0080050 GO:0080090 GO:0080167 GO:0097159 GO:0097305 GO:0140110 GO:1901363 GO:1901419 GO:1901568 GO:1901570 GO:1901576 GO:1901700 GO:1902680 GO:1902930 GO:1903506 GO:1903508 GO:1904276 GO:1904278 GO:1905957 GO:2000026 GO:2000033 GO:2000034 GO:2000112 GO:2000241 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

330

Amino Acids

36.97

Weight (kDa)

6.32

Isoelectric Point (pI)

49.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 14 - 61 8.4e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 17 - 76 1.1e-10 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 67 - 112 2e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 116 8.8e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023956)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74650 AT1G74650 AT1G74650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 7
AclWI GGATC 4 cut(s) 200, 553, 563, 781
AcoI YGGCCR 1 cut(s) 507
AcsI RAATTY 3 cut(s) 205, 760, 793
AfiI CCNNNNNNNGG 2 cut(s) 702, 722
AflIII ACRYGT 1 cut(s) 969
AgeI ACCGGT 1 cut(s) 131
AgsI TTSAA 4 cut(s) 245, 365, 491, 820
AhlI ACTAGT 1 cut(s) 346
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 6 cut(s) 248, 272, 660, 708, 790, 854
AluI AGCT 6 cut(s) 248, 272, 660, 708, 790, 854
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 3 cut(s) 82, 697, 800
AlwI GGATC 4 cut(s) 200, 553, 563, 781
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 3 cut(s) 97, 469, 507
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 3 cut(s) 205, 760, 793
AsiGI ACCGGT 1 cut(s) 131
AspS9I GGNCC 4 cut(s) 44, 98, 297, 456
AsuC2I CCSGG 3 cut(s) 189, 190, 717
AsuHPI GGTGA 4 cut(s) 46, 570, 731, 836
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 3 cut(s) 44, 297, 456
BalI TGGCCA 1 cut(s) 509
BanII GRGCYC 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 710
BbvI GCAGC 1 cut(s) 281
BccI CCATC 3 cut(s) 162, 258, 556
BceAI ACGGC 1 cut(s) 112
BciT130I CCWGG 1 cut(s) 102
BclI TGATCA 4 cut(s) 231, 736, 880, 922
BcnI CCSGG 3 cut(s) 189, 190, 717
BcoDI GTCTC 3 cut(s) 82, 697, 800
BcuI ACTAGT 1 cut(s) 346
BfaI CTAG 1 cut(s) 347
BfmI CTRYAG 1 cut(s) 273
BglII AGATCT 2 cut(s) 113, 958
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 4 cut(s) 102, 189, 190, 717
Bme18I GGWCC 3 cut(s) 44, 297, 456
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 4 cut(s) 44, 98, 297, 456
BmrFI CCNGG 4 cut(s) 102, 189, 190, 717
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 629
Bpu10I CCTNAGC 1 cut(s) 140
BpuEI CTTGAG 2 cut(s) 601, 694
BpuMI CCSGG 3 cut(s) 189, 190, 717
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 47, 100, 188, 715
BsaWI WCCGGW 3 cut(s) 131, 217, 702
Bsc4I CCNNNNNNNGG 2 cut(s) 702, 722
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 2 cut(s) 326, 656
Bse8I GATNNNNATC 1 cut(s) 233
BseBI CCWGG 1 cut(s) 102
BseDI CCNNGG 4 cut(s) 47, 100, 188, 715
BseGI GGATG 2 cut(s) 234, 443
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 702, 722
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 2 cut(s) 326, 656
BseXI GCAGC 1 cut(s) 281
BshFI GGCC 3 cut(s) 99, 471, 509
BshTI ACCGGT 1 cut(s) 131
BsiHKAI GWGCWC 1 cut(s) 710
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 5 cut(s) 132, 189, 218, 703, 717
BslFI GGGAC 2 cut(s) 104, 171
BslI CCNNNNNNNGG 2 cut(s) 702, 722
BsmAI GTCTC 3 cut(s) 82, 697, 800
BsmFI GGGAC 2 cut(s) 104, 171
BsnI GGCC 3 cut(s) 99, 471, 509
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 710
Bsp19I CCATGG 1 cut(s) 47
BspANI GGCC 3 cut(s) 99, 471, 509
BspCNI CTCAG 1 cut(s) 304
BspPI GGATC 4 cut(s) 200, 553, 563, 781
BspTNI GGTCTC 1 cut(s) 82
BsrFI RCCGGY 1 cut(s) 131
BsrI ACTGG 2 cut(s) 326, 656
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 4 cut(s) 47, 100, 188, 715
BssT1I CCWWGG 1 cut(s) 47
Bst2UI CCWGG 1 cut(s) 102
Bst6I CTCTTC 1 cut(s) 216
BstAPI GCANNNNNTGC 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 833
BstDEI CTNAG 3 cut(s) 140, 164, 291
BstDSI CCRYGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 234, 443
BstMAI GTCTC 3 cut(s) 82, 697, 800
BstMWI GCNNNNNNNGC 3 cut(s) 156, 275, 515
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 4 cut(s) 100, 187, 188, 715
BstSFI CTRYAG 1 cut(s) 273
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 69
BstX2I RGATCY 2 cut(s) 113, 958
BstYI RGATCY 2 cut(s) 113, 958
BsuRI GGCC 3 cut(s) 99, 471, 509
BtgI CCRYGG 1 cut(s) 47
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 2 cut(s) 234, 443
BtsIMutI CAGTG 1 cut(s) 944
Cac8I GCNNGC 1 cut(s) 833
CaiI CAGNNNCTG 1 cut(s) 770
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 4 cut(s) 44, 98, 297, 456
Cfr9I CCCGGG 1 cut(s) 188
CspAI ACCGGT 1 cut(s) 131
CviAII CATG 4 cut(s) 48, 505, 814, 847
DdeI CTNAG 3 cut(s) 140, 164, 291
EaeI YGGCCR 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
Ecl136II GAGCTC 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 47
Eco24I GRGCYC 1 cut(s) 710
Eco31I GGTCTC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 310
Eco47I GGWCC 3 cut(s) 44, 297, 456
Eco53kI GAGCTC 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 708
EcoRI GAATTC 1 cut(s) 760
EcoRII CCWGG 1 cut(s) 100
EcoRV GATATC 1 cut(s) 310
EcoT14I CCWWGG 1 cut(s) 47
EcoT38I GRGCYC 1 cut(s) 710
ErhI CCWWGG 1 cut(s) 47
FaeI CATG 4 cut(s) 51, 508, 817, 850
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 2 cut(s) 104, 171
FatI CATG 4 cut(s) 47, 504, 813, 846
FbaI TGATCA 4 cut(s) 231, 736, 880, 922
FblI GTMKAC 1 cut(s) 7
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 2 cut(s) 221, 450
FriOI GRGCYC 1 cut(s) 710
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 1 cut(s) 347
GluI GCNGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 629
HaeIII GGCC 3 cut(s) 99, 471, 509
HapII CCGG 5 cut(s) 132, 189, 218, 703, 717
Hin1II CATG 4 cut(s) 51, 508, 817, 850
HinfI GANTC 3 cut(s) 52, 575, 767
HpaII CCGG 5 cut(s) 132, 189, 218, 703, 717
HphI GGTGA 4 cut(s) 46, 570, 731, 836
Hpy166II GTNNAC 1 cut(s) 8
Hpy188I TCNGA 3 cut(s) 294, 766, 805
Hpy188III TCNNGA 8 cut(s) 56, 313, 566, 572, 646, 771, 904, 953
Hpy8I GTNNAC 1 cut(s) 8
Hpy99I CGWCG 1 cut(s) 971
HpyAV CCTTC 3 cut(s) 191, 251, 716
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 2 cut(s) 125, 159
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 275, 515
HpyF3I CTNAG 3 cut(s) 140, 164, 291
HpySE526I ACGT 1 cut(s) 969
Hsp92II CATG 4 cut(s) 51, 508, 817, 850
Ksp22I TGATCA 4 cut(s) 231, 736, 880, 922
LmnI GCTCC 1 cut(s) 705
Lsp1109I GCAGC 1 cut(s) 281
LweI GCATC 1 cut(s) 287
MaeI CTAG 1 cut(s) 347
MaeII ACGT 1 cut(s) 969
MaeIII GTNAC 1 cut(s) 534
MboII GAAGA 8 cut(s) 37, 49, 71, 74, 233, 238, 543, 968
MflI RGATCY 2 cut(s) 113, 958
MhlI GDGCHC 1 cut(s) 710
MlsI TGGCCA 1 cut(s) 509
MluCI AATT 6 cut(s) 106, 175, 205, 638, 760, 793
MluNI TGGCCA 1 cut(s) 509
MlyI GAGTC 2 cut(s) 46, 569
MmeI TCCRAC 2 cut(s) 112, 720
MnlI CCTC 9 cut(s) 25, 194, 288, 300, 573, 597, 625, 719, 973
Mox20I TGGCCA 1 cut(s) 509
MscI TGGCCA 1 cut(s) 509
MseI TTAA 2 cut(s) 336, 986
MslI CAYNNNNRTG 3 cut(s) 503, 845, 968
Msp20I TGGCCA 1 cut(s) 509
MspI CCGG 5 cut(s) 132, 189, 218, 703, 717
MspR9I CCNGG 4 cut(s) 102, 189, 190, 717
MvaI CCWGG 1 cut(s) 102
MwoI GCNNNNNNNGC 3 cut(s) 156, 275, 515
NciI CCSGG 3 cut(s) 189, 190, 717
NcoI CCATGG 1 cut(s) 47
NlaIII CATG 4 cut(s) 51, 508, 817, 850
NmuCI GTSAC 1 cut(s) 534
NspI RCATGY 1 cut(s) 817
PfeI GAWTC 1 cut(s) 767
PinAI ACCGGT 1 cut(s) 131
PkrI GCNGC 1 cut(s) 271
PleI GAGTC 2 cut(s) 46, 569
PpsI GAGTC 2 cut(s) 46, 569
Psp124BI GAGCTC 1 cut(s) 710
Psp6I CCWGG 1 cut(s) 100
PspGI CCWGG 1 cut(s) 100
PspPI GGNCC 4 cut(s) 44, 98, 297, 456
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 2 cut(s) 113, 958
RseI CAYNNNNRTG 3 cut(s) 503, 845, 968
SacI GAGCTC 1 cut(s) 710
SaqAI TTAA 2 cut(s) 336, 986
SatI GCNGC 1 cut(s) 270
Sau96I GGNCC 4 cut(s) 44, 98, 297, 456
SchI GAGTC 2 cut(s) 46, 569
ScrFI CCNGG 4 cut(s) 102, 189, 190, 717
SduI GDGCHC 1 cut(s) 710
SfaNI GCATC 1 cut(s) 287
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 3 cut(s) 44, 297, 456
SmaI CCCGGG 1 cut(s) 190
SmiMI CAYNNNNRTG 3 cut(s) 503, 845, 968
SmlI CTYRAG 2 cut(s) 616, 709
SmoI CTYRAG 2 cut(s) 616, 709
SpeI ACTAGT 1 cut(s) 346
Sse9I AATT 6 cut(s) 106, 175, 205, 638, 760, 793
SspMI CTAG 1 cut(s) 347
SstI GAGCTC 1 cut(s) 710
StyD4I CCNGG 4 cut(s) 100, 187, 188, 715
StyI CCWWGG 1 cut(s) 47
TaiI ACGT 1 cut(s) 972
TaqI TCGA 3 cut(s) 758, 903, 966
TaqII GACCGA 1 cut(s) 314
TasI AATT 6 cut(s) 106, 175, 205, 638, 760, 793
TfiI GAWTC 1 cut(s) 767
Tru1I TTAA 2 cut(s) 336, 986
Tru9I TTAA 2 cut(s) 336, 986
TscAI CASTG 1 cut(s) 944
TseFI GTSAC 1 cut(s) 534
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 534
TspDTI ATGAA 3 cut(s) 371, 543, 675
TspMI CCCGGG 1 cut(s) 188
TspRI CASTG 1 cut(s) 944
VpaK11BI GGWCC 3 cut(s) 44, 297, 456
XapI RAATTY 3 cut(s) 205, 760, 793
XceI RCATGY 1 cut(s) 817
XmaI CCCGGG 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 7
XspI CTAG 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.