Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1137, 1283 |
| AccI | GTMKAC | 3 cut(s) | 1025, 1984, 2092 |
| AccII | CGCG | 2 cut(s) | 1500, 2305 |
| AciI | CCGC | 5 cut(s) | 102, 947, 981, 1500, 2045 |
| AclI | AACGTT | 1 cut(s) | 1212 |
| AclWI | GGATC | 4 cut(s) | 1160, 1475, 2192, 2382 |
| AcoI | YGGCCR | 2 cut(s) | 1557, 2281 |
| AcsI | RAATTY | 5 cut(s) | 588, 1361, 1808, 1852, 2548 |
| AcuI | CTGAAG | 1 cut(s) | 2583 |
| AcvI | CACGTG | 1 cut(s) | 1849 |
| AdeI | CACNNNGTG | 2 cut(s) | 468, 1849 |
| AfaI | GTAC | 4 cut(s) | 627, 1176, 2024, 2074 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 801 |
| AflII | CTTAAG | 1 cut(s) | 1399 |
| AjiI | CACGTC | 1 cut(s) | 460 |
| AjnI | CCWGG | 4 cut(s) | 800, 897, 1520, 1978 |
| AluBI | AGCT | 9 cut(s) | 19, 1006, 1155, 1206, 1997, 2250, 2420, 2494, 2603 |
| AluI | AGCT | 9 cut(s) | 19, 1006, 1155, 1206, 1997, 2250, 2420, 2494, 2603 |
| Alw26I | GTCTC | 3 cut(s) | 72, 494, 2458 |
| AlwI | GGATC | 4 cut(s) | 1160, 1475, 2192, 2382 |
| Ama87I | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| AoxI | GGCC | 7 cut(s) | 233, 870, 1557, 1960, 1976, 2281, 2331 |
| ApeKI | GCWGC | 3 cut(s) | 968, 1003, 1206 |
| ApoI | RAATTY | 5 cut(s) | 588, 1361, 1808, 1852, 2548 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 885, 917, 1806, 1838 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 574, 1704, 1830 |
| AspLEI | GCGC | 1 cut(s) | 1500 |
| AspS9I | GGNCC | 3 cut(s) | 386, 1961, 2331 |
| AsuC2I | CCSGG | 1 cut(s) | 703 |
| AsuHPI | GGTGA | 5 cut(s) | 443, 598, 952, 1835, 2366 |
| AvaI | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| AvaII | GGWCC | 1 cut(s) | 386 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2145, 2178 |
| BalI | TGGCCA | 1 cut(s) | 2283 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1812, 1844 |
| BbrPI | CACGTG | 1 cut(s) | 1849 |
| BbsI | GAAGAC | 1 cut(s) | 1251 |
| BbvI | GCAGC | 3 cut(s) | 980, 1015, 1193 |
| BccI | CCATC | 7 cut(s) | 419, 428, 719, 1189, 1454, 1642, 2394 |
| BceAI | ACGGC | 2 cut(s) | 1544, 1751 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 815, 849 |
| BciT130I | CCWGG | 4 cut(s) | 802, 899, 1522, 1980 |
| BciVI | GTATCC | 1 cut(s) | 1865 |
| BcnI | CCSGG | 1 cut(s) | 703 |
| BcoDI | GTCTC | 3 cut(s) | 72, 494, 2458 |
| BfaI | CTAG | 5 cut(s) | 882, 1290, 1367, 2012, 2141 |
| BfmI | CTRYAG | 3 cut(s) | 280, 966, 1050 |
| BfrI | CTTAAG | 1 cut(s) | 1399 |
| BfuI | GTATCC | 1 cut(s) | 1865 |
| BglII | AGATCT | 1 cut(s) | 2132 |
| BisI | GCNGC | 4 cut(s) | 969, 1004, 1207, 2045 |
| BlsI | GCNGC | 4 cut(s) | 970, 1005, 1208, 2046 |
| BmcAI | AGTACT | 1 cut(s) | 2024 |
| Bme1390I | CCNGG | 5 cut(s) | 703, 802, 899, 1522, 1980 |
| Bme18I | GGWCC | 1 cut(s) | 386 |
| BmeT110I | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| BmgBI | CACGTC | 1 cut(s) | 460 |
| BmgT120I | GGNCC | 3 cut(s) | 386, 1961, 2331 |
| BmiI | GGNNCC | 2 cut(s) | 577, 2537 |
| BmrFI | CCNGG | 5 cut(s) | 703, 802, 899, 1522, 1980 |
| BmsI | GCATC | 1 cut(s) | 1550 |
| BoxI | GACNNNNGTC | 1 cut(s) | 486 |
| BpiI | GAAGAC | 1 cut(s) | 1251 |
| BpmI | CTGGAG | 2 cut(s) | 1296, 1368 |
| BpuEI | CTTGAG | 2 cut(s) | 1416, 1779 |
| BpuMI | CCSGG | 1 cut(s) | 703 |
| BsaAI | YACGTR | 1 cut(s) | 1849 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2379 |
| BsaI | GGTCTC | 1 cut(s) | 2458 |
| BsaJI | CCNNGG | 2 cut(s) | 932, 2562 |
| BsaWI | WCCGGW | 1 cut(s) | 676 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 801 |
| Bse1I | ACTGG | 4 cut(s) | 196, 1279, 1351, 2284 |
| Bse3DI | GCAATG | 1 cut(s) | 1107 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2379 |
| BseBI | CCWGG | 4 cut(s) | 802, 899, 1522, 1980 |
| BseDI | CCNNGG | 2 cut(s) | 932, 2562 |
| BseGI | GGATG | 5 cut(s) | 430, 810, 1105, 1200, 2042 |
| BseJI | GATNNNNATC | 1 cut(s) | 2379 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 801 |
| BseMI | GCAATG | 1 cut(s) | 1107 |
| BseMII | CTCAG | 3 cut(s) | 225, 843, 2453 |
| BseNI | ACTGG | 4 cut(s) | 196, 1279, 1351, 2284 |
| BseRI | GAGGAG | 2 cut(s) | 195, 461 |
| BseXI | GCAGC | 3 cut(s) | 980, 1015, 1193 |
| BsgI | GTGCAG | 2 cut(s) | 1022, 1992 |
| Bsh1236I | CGCG | 2 cut(s) | 1500, 2305 |
| BshFI | GGCC | 7 cut(s) | 235, 872, 1559, 1962, 1978, 2283, 2333 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| BsiSI | CCGG | 2 cut(s) | 677, 703 |
| BslFI | GGGAC | 2 cut(s) | 517, 2580 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 801 |
| BsmAI | GTCTC | 3 cut(s) | 72, 494, 2458 |
| BsmFI | GGGAC | 2 cut(s) | 517, 2580 |
| BsmI | GAATGC | 1 cut(s) | 1051 |
| BsnI | GGCC | 7 cut(s) | 235, 872, 1559, 1962, 1978, 2283, 2333 |
| Bso31I | GGTCTC | 1 cut(s) | 2458 |
| BsoBI | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| Bsp143I | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| BspACI | CCGC | 5 cut(s) | 102, 947, 981, 1500, 2045 |
| BspANI | GGCC | 7 cut(s) | 235, 872, 1559, 1962, 1978, 2283, 2333 |
| BspCNI | CTCAG | 3 cut(s) | 224, 844, 2454 |
| BspFNI | CGCG | 2 cut(s) | 1500, 2305 |
| BspHI | TCATGA | 1 cut(s) | 2488 |
| BspLI | GGNNCC | 2 cut(s) | 577, 2537 |
| BspMAI | CTGCAG | 1 cut(s) | 970 |
| BspPI | GGATC | 4 cut(s) | 1160, 1475, 2192, 2382 |
| BspTI | CTTAAG | 1 cut(s) | 1399 |
| BspTNI | GGTCTC | 1 cut(s) | 2458 |
| BsrDI | GCAATG | 1 cut(s) | 1107 |
| BsrI | ACTGG | 4 cut(s) | 196, 1279, 1351, 2284 |
| BssECI | CCNNGG | 2 cut(s) | 932, 2562 |
| BssMI | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| BssT1I | CCWWGG | 1 cut(s) | 932 |
| Bst2UI | CCWGG | 4 cut(s) | 802, 899, 1522, 1980 |
| Bst4CI | ACNGT | 8 cut(s) | 284, 350, 1185, 1383, 1877, 2085, 2097, 2575 |
| Bst6I | CTCTTC | 1 cut(s) | 2228 |
| BstAFI | CTTAAG | 1 cut(s) | 1399 |
| BstBAI | YACGTR | 1 cut(s) | 1849 |
| BstC8I | GCNNGC | 3 cut(s) | 17, 1157, 2346 |
| BstDEI | CTNAG | 5 cut(s) | 211, 852, 1415, 1835, 2462 |
| BstF5I | GGATG | 5 cut(s) | 430, 810, 1105, 1200, 2042 |
| BstFNI | CGCG | 2 cut(s) | 1500, 2305 |
| BstHHI | GCGC | 1 cut(s) | 1500 |
| BstKTI | GATC | 9 cut(s) | 453, 652, 1168, 1377, 1483, 2135, 2200, 2377, 2545 |
| BstMAI | GTCTC | 3 cut(s) | 72, 494, 2458 |
| BstMBI | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 1161, 1538, 2111 |
| BstNI | CCWGG | 4 cut(s) | 802, 899, 1522, 1980 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 486 |
| BstSCI | CCNGG | 5 cut(s) | 701, 800, 897, 1520, 1978 |
| BstSFI | CTRYAG | 3 cut(s) | 280, 966, 1050 |
| BstUI | CGCG | 2 cut(s) | 1500, 2305 |
| BstV1I | GCAGC | 3 cut(s) | 980, 1015, 1193 |
| BstV2I | GAAGAC | 1 cut(s) | 1251 |
| BstX2I | RGATCY | 1 cut(s) | 2132 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 396, 1521 |
| BstYI | RGATCY | 1 cut(s) | 2132 |
| BsuI | GTATCC | 1 cut(s) | 1865 |
| BsuRI | GGCC | 7 cut(s) | 235, 872, 1559, 1962, 1978, 2283, 2333 |
| BtgZI | GCGATG | 1 cut(s) | 2447 |
| BtrI | CACGTC | 1 cut(s) | 460 |
| BtsCI | GGATG | 5 cut(s) | 430, 810, 1105, 1200, 2042 |
| BtsIMutI | CAGTG | 4 cut(s) | 787, 2277, 2459, 2593 |
| Cac8I | GCNNGC | 3 cut(s) | 17, 1157, 2346 |
| CciI | TCATGA | 1 cut(s) | 2488 |
| CfoI | GCGC | 1 cut(s) | 1500 |
| Cfr13I | GGNCC | 3 cut(s) | 386, 1961, 2331 |
| CseI | GACGC | 1 cut(s) | 1054 |
| Csp6I | GTAC | 4 cut(s) | 626, 1175, 2023, 2073 |
| CviAII | CATG | 7 cut(s) | 1170, 1191, 1739, 1915, 2159, 2473, 2489 |
| CviQI | GTAC | 4 cut(s) | 626, 1175, 2023, 2073 |
| DdeI | CTNAG | 5 cut(s) | 211, 852, 1415, 1835, 2462 |
| DpnI | GATC | 9 cut(s) | 452, 651, 1167, 1376, 1482, 2134, 2199, 2376, 2544 |
| DpnII | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| DraI | TTTAAA | 1 cut(s) | 1467 |
| DraIII | CACNNNGTG | 2 cut(s) | 468, 1849 |
| EaeI | YGGCCR | 2 cut(s) | 1557, 2281 |
| Eam1104I | CTCTTC | 1 cut(s) | 2228 |
| EarI | CTCTTC | 1 cut(s) | 2228 |
| Eco130I | CCWWGG | 1 cut(s) | 932 |
| Eco31I | GGTCTC | 1 cut(s) | 2458 |
| Eco32I | GATATC | 3 cut(s) | 522, 2192, 2593 |
| Eco47I | GGWCC | 1 cut(s) | 386 |
| Eco57I | CTGAAG | 1 cut(s) | 2583 |
| Eco72I | CACGTG | 1 cut(s) | 1849 |
| Eco88I | CYCGRG | 3 cut(s) | 1945, 2478, 2563 |
| EcoRI | GAATTC | 1 cut(s) | 1808 |
| EcoRII | CCWGG | 4 cut(s) | 800, 897, 1520, 1978 |
| EcoRV | GATATC | 3 cut(s) | 522, 2192, 2593 |
| EcoT14I | CCWWGG | 1 cut(s) | 932 |
| ErhI | CCWWGG | 1 cut(s) | 932 |
| FaeI | CATG | 7 cut(s) | 1173, 1194, 1742, 1918, 2162, 2476, 2492 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2094, 2126, 2395, 2427 |
| FaqI | GGGAC | 2 cut(s) | 517, 2580 |
| FatI | CATG | 7 cut(s) | 1169, 1190, 1738, 1914, 2158, 2472, 2488 |
| FauI | CCCGC | 2 cut(s) | 974, 1493 |
| FauNDI | CATATG | 1 cut(s) | 485 |
| FblI | GTMKAC | 3 cut(s) | 1025, 1984, 2092 |
| Fnu4HI | GCNGC | 4 cut(s) | 969, 1004, 1207, 2045 |
| FokI | GGATG | 5 cut(s) | 437, 817, 1112, 1207, 2049 |
| Fsp4HI | GCNGC | 4 cut(s) | 969, 1004, 1207, 2045 |
| FspBI | CTAG | 5 cut(s) | 882, 1290, 1367, 2012, 2141 |
| GlaI | GCGC | 1 cut(s) | 1499 |
| GluI | GCNGC | 4 cut(s) | 969, 1004, 1207, 2045 |
| GsuI | CTGGAG | 2 cut(s) | 1296, 1368 |
| HaeIII | GGCC | 7 cut(s) | 235, 872, 1559, 1962, 1978, 2283, 2333 |
| HapII | CCGG | 2 cut(s) | 677, 703 |
| HgaI | GACGC | 1 cut(s) | 1054 |
| HhaI | GCGC | 1 cut(s) | 1500 |
| Hin1II | CATG | 7 cut(s) | 1173, 1194, 1742, 1918, 2162, 2476, 2492 |
| Hin6I | GCGC | 1 cut(s) | 1498 |
| HinP1I | GCGC | 1 cut(s) | 1498 |
| HincII | GTYRAC | 2 cut(s) | 1026, 2226 |
| HindII | GTYRAC | 2 cut(s) | 1026, 2226 |
| HindIII | AAGCTT | 1 cut(s) | 17 |
| HpaI | GTTAAC | 1 cut(s) | 2226 |
| HpaII | CCGG | 2 cut(s) | 677, 703 |
| HphI | GGTGA | 5 cut(s) | 443, 598, 952, 1835, 2366 |
| Hpy166II | GTNNAC | 7 cut(s) | 1026, 1175, 1843, 1985, 2056, 2093, 2226 |
| Hpy8I | GTNNAC | 7 cut(s) | 1026, 1175, 1843, 1985, 2056, 2093, 2226 |
| HpyAV | CCTTC | 7 cut(s) | 120, 257, 1448, 1593, 2460, 2526, 2607 |
| HpyCH4III | ACNGT | 8 cut(s) | 284, 350, 1185, 1383, 1877, 2085, 2097, 2575 |
| HpyCH4IV | ACGT | 6 cut(s) | 459, 628, 1212, 1630, 1848, 1954 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 1161, 1538, 2111 |
| HpyF3I | CTNAG | 5 cut(s) | 211, 852, 1415, 1835, 2462 |
| HpySE526I | ACGT | 6 cut(s) | 459, 628, 1212, 1630, 1848, 1954 |
| Hsp92II | CATG | 7 cut(s) | 1173, 1194, 1742, 1918, 2162, 2476, 2492 |
| HspAI | GCGC | 1 cut(s) | 1498 |
| KspAI | GTTAAC | 1 cut(s) | 2226 |
| Kzo9I | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| LmnI | GCTCC | 1 cut(s) | 2535 |
| Lsp1109I | GCAGC | 3 cut(s) | 980, 1015, 1193 |
| LweI | GCATC | 1 cut(s) | 1550 |
| MaeI | CTAG | 5 cut(s) | 882, 1290, 1367, 2012, 2141 |
| MaeII | ACGT | 6 cut(s) | 459, 628, 1212, 1630, 1848, 1954 |
| MaeIII | GTNAC | 4 cut(s) | 460, 1561, 2262, 2575 |
| MalI | GATC | 9 cut(s) | 452, 651, 1167, 1376, 1482, 2134, 2199, 2376, 2544 |
| MboI | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| MboII | GAAGA | 6 cut(s) | 650, 1251, 1579, 1668, 2245, 2445 |
| MflI | RGATCY | 1 cut(s) | 2132 |
| MlsI | TGGCCA | 1 cut(s) | 2283 |
| MluNI | TGGCCA | 1 cut(s) | 2283 |
| MlyI | GAGTC | 4 cut(s) | 679, 1021, 2012, 2470 |
| MmeI | TCCRAC | 3 cut(s) | 138, 789, 1849 |
| Mox20I | TGGCCA | 1 cut(s) | 2283 |
| MroXI | GAANNNNTTC | 3 cut(s) | 574, 1704, 1830 |
| MscI | TGGCCA | 1 cut(s) | 2283 |
| MslI | CAYNNNNRTG | 2 cut(s) | 976, 2061 |
| Msp20I | TGGCCA | 1 cut(s) | 2283 |
| MspA1I | CMGCKG | 1 cut(s) | 1155 |
| MspCI | CTTAAG | 1 cut(s) | 1399 |
| MspI | CCGG | 2 cut(s) | 677, 703 |
| MspR9I | CCNGG | 5 cut(s) | 703, 802, 899, 1522, 1980 |
| Mva1269I | GAATGC | 1 cut(s) | 1051 |
| MvaI | CCWGG | 4 cut(s) | 802, 899, 1522, 1980 |
| MvnI | CGCG | 2 cut(s) | 1500, 2305 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 1161, 1538, 2111 |
| NciI | CCSGG | 1 cut(s) | 703 |
| NdeI | CATATG | 1 cut(s) | 485 |
| NdeII | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| NlaIII | CATG | 7 cut(s) | 1173, 1194, 1742, 1918, 2162, 2476, 2492 |
| NlaIV | GGNNCC | 2 cut(s) | 577, 2537 |
| NmuCI | GTSAC | 1 cut(s) | 460 |
| PagI | TCATGA | 1 cut(s) | 2488 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1379 |
| PctI | GAATGC | 1 cut(s) | 1051 |
| PdmI | GAANNNNTTC | 3 cut(s) | 574, 1704, 1830 |
| PfeI | GAWTC | 8 cut(s) | 84, 119, 661, 812, 856, 1149, 1239, 1300 |
| PkrI | GCNGC | 4 cut(s) | 970, 1005, 1208, 2046 |
| PleI | GAGTC | 4 cut(s) | 679, 1021, 2011, 2470 |
| PmaCI | CACGTG | 1 cut(s) | 1849 |
| PmlI | CACGTG | 1 cut(s) | 1849 |
| PpsI | GAGTC | 4 cut(s) | 679, 1021, 2011, 2470 |
| Ppu21I | YACGTR | 1 cut(s) | 1849 |
| PshAI | GACNNNNGTC | 1 cut(s) | 486 |
| PsiI | TTATAA | 2 cut(s) | 1137, 1283 |
| Psp1406I | AACGTT | 1 cut(s) | 1212 |
| Psp6I | CCWGG | 4 cut(s) | 800, 897, 1520, 1978 |
| PspCI | CACGTG | 1 cut(s) | 1849 |
| PspGI | CCWGG | 4 cut(s) | 800, 897, 1520, 1978 |
| PspN4I | GGNNCC | 2 cut(s) | 577, 2537 |
| PspPI | GGNCC | 3 cut(s) | 386, 1961, 2331 |
| PstI | CTGCAG | 1 cut(s) | 970 |
| PsuI | RGATCY | 1 cut(s) | 2132 |
| PvuII | CAGCTG | 1 cut(s) | 1155 |
| RsaI | GTAC | 4 cut(s) | 627, 1176, 2024, 2074 |
| RsaNI | GTAC | 4 cut(s) | 626, 1175, 2023, 2073 |
| RseI | CAYNNNNRTG | 2 cut(s) | 976, 2061 |
| SalI | GTCGAC | 1 cut(s) | 1024 |
| SatI | GCNGC | 4 cut(s) | 969, 1004, 1207, 2045 |
| Sau3AI | GATC | 9 cut(s) | 450, 649, 1165, 1374, 1480, 2132, 2197, 2374, 2542 |
| Sau96I | GGNCC | 3 cut(s) | 386, 1961, 2331 |
| ScaI | AGTACT | 1 cut(s) | 2024 |
| SchI | GAGTC | 4 cut(s) | 679, 1021, 2012, 2470 |
| ScrFI | CCNGG | 5 cut(s) | 703, 802, 899, 1522, 1980 |
| SfaNI | GCATC | 1 cut(s) | 1550 |
| SfcI | CTRYAG | 3 cut(s) | 280, 966, 1050 |
| SinI | GGWCC | 1 cut(s) | 386 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 976, 2061 |
| SmlI | CTYRAG | 3 cut(s) | 1399, 1431, 1794 |
| SmoI | CTYRAG | 3 cut(s) | 1399, 1431, 1794 |
| SsiI | CCGC | 5 cut(s) | 102, 947, 981, 1500, 2045 |
| SspI | AATATT | 2 cut(s) | 394, 2207 |
| SspMI | CTAG | 5 cut(s) | 882, 1290, 1367, 2012, 2141 |
| StyD4I | CCNGG | 5 cut(s) | 701, 800, 897, 1520, 1978 |
| StyI | CCWWGG | 1 cut(s) | 932 |
| TaaI | ACNGT | 8 cut(s) | 284, 350, 1185, 1383, 1877, 2085, 2097, 2575 |
| TaiI | ACGT | 6 cut(s) | 462, 631, 1215, 1633, 1851, 1957 |
| TaqI | TCGA | 9 cut(s) | 132, 230, 444, 568, 1025, 1262, 1315, 1824, 2377 |
| TaqII | GACCGA | 1 cut(s) | 374 |
| TatI | WGTACW | 1 cut(s) | 2022 |
| TauI | GCSGC | 1 cut(s) | 2047 |
| TfiI | GAWTC | 8 cut(s) | 84, 119, 661, 812, 856, 1149, 1239, 1300 |
| TscAI | CASTG | 4 cut(s) | 787, 2284, 2466, 2593 |
| TseFI | GTSAC | 1 cut(s) | 460 |
| TseI | GCWGC | 3 cut(s) | 968, 1003, 1206 |
| Tsp45I | GTSAC | 1 cut(s) | 460 |
| TspGWI | ACGGA | 1 cut(s) | 1112 |
| TspRI | CASTG | 4 cut(s) | 787, 2284, 2466, 2593 |
| Vha464I | CTTAAG | 1 cut(s) | 1399 |
| VpaK11BI | GGWCC | 1 cut(s) | 386 |
| XapI | RAATTY | 5 cut(s) | 588, 1361, 1808, 1852, 2548 |
| XmiI | GTMKAC | 3 cut(s) | 1025, 1984, 2092 |
| XmnI | GAANNNNTTC | 3 cut(s) | 574, 1704, 1830 |
| XspI | CTAG | 5 cut(s) | 882, 1290, 1367, 2012, 2141 |
| ZrmI | AGTACT | 1 cut(s) | 2024 |