nuclease activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 32 |
| AccB1I | GGYRCC | 1 cut(s) | 32 |
| AclWI | GGATC | 1 cut(s) | 215 |
| AcsI | RAATTY | 1 cut(s) | 200 |
| AfaI | GTAC | 1 cut(s) | 34 |
| AgsI | TTSAA | 1 cut(s) | 80 |
| AluBI | AGCT | 1 cut(s) | 100 |
| AluI | AGCT | 1 cut(s) | 100 |
| Alw26I | GTCTC | 1 cut(s) | 136 |
| AlwI | GGATC | 1 cut(s) | 215 |
| ApoI | RAATTY | 1 cut(s) | 200 |
| Asp718I | GGTACC | 1 cut(s) | 32 |
| BanI | GGYRCC | 1 cut(s) | 32 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 26, 60 |
| BcoDI | GTCTC | 1 cut(s) | 136 |
| BmiI | GGNNCC | 1 cut(s) | 34 |
| BmsI | GCATC | 1 cut(s) | 124 |
| BseRI | GAGGAG | 1 cut(s) | 107 |
| BseYI | CCCAGC | 1 cut(s) | 137 |
| BshNI | GGYRCC | 1 cut(s) | 32 |
| BsmAI | GTCTC | 1 cut(s) | 136 |
| Bsp143I | GATC | 1 cut(s) | 207 |
| BspLI | GGNNCC | 1 cut(s) | 34 |
| BspPI | GGATC | 1 cut(s) | 215 |
| BspT107I | GGYRCC | 1 cut(s) | 32 |
| BssMI | GATC | 1 cut(s) | 207 |
| BstDEI | CTNAG | 2 cut(s) | 12, 83 |
| BstKTI | GATC | 1 cut(s) | 210 |
| BstMAI | GTCTC | 1 cut(s) | 136 |
| BstMBI | GATC | 1 cut(s) | 207 |
| Csp6I | GTAC | 1 cut(s) | 33 |
| CviAII | CATG | 1 cut(s) | 192 |
| CviJI | RGCY | 1 cut(s) | 100 |
| CviKI_1 | RGCY | 1 cut(s) | 100 |
| CviQI | GTAC | 1 cut(s) | 33 |
| DdeI | CTNAG | 2 cut(s) | 12, 83 |
| DpnI | GATC | 1 cut(s) | 209 |
| DpnII | GATC | 1 cut(s) | 207 |
| FaeI | CATG | 1 cut(s) | 195 |
| FaiI | YATR | 6 cut(s) | 155, 168, 177, 189, 193, 237 |
| FatI | CATG | 1 cut(s) | 191 |
| GsaI | CCCAGC | 1 cut(s) | 141 |
| Hin1II | CATG | 1 cut(s) | 195 |
| HindIII | AAGCTT | 1 cut(s) | 98 |
| HinfI | GANTC | 1 cut(s) | 118 |
| Hpy166II | GTNNAC | 1 cut(s) | 216 |
| Hpy188I | TCNGA | 2 cut(s) | 123, 254 |
| Hpy8I | GTNNAC | 1 cut(s) | 216 |
| HpyAV | CCTTC | 1 cut(s) | 233 |
| HpyF3I | CTNAG | 2 cut(s) | 12, 83 |
| Hsp92II | CATG | 1 cut(s) | 195 |
| KpnI | GGTACC | 1 cut(s) | 36 |
| Kzo9I | GATC | 1 cut(s) | 207 |
| LpnPI | CCDG | 1 cut(s) | 123 |
| LweI | GCATC | 1 cut(s) | 124 |
| MalI | GATC | 1 cut(s) | 209 |
| MboI | GATC | 1 cut(s) | 207 |
| MluCI | AATT | 2 cut(s) | 87, 200 |
| MnlI | CCTC | 2 cut(s) | 85, 163 |
| MseI | TTAA | 1 cut(s) | 102 |
| NdeII | GATC | 1 cut(s) | 207 |
| NlaIII | CATG | 1 cut(s) | 195 |
| NlaIV | GGNNCC | 1 cut(s) | 34 |
| PfeI | GAWTC | 1 cut(s) | 118 |
| PspFI | CCCAGC | 1 cut(s) | 137 |
| PspN4I | GGNNCC | 1 cut(s) | 34 |
| RsaI | GTAC | 1 cut(s) | 34 |
| RsaNI | GTAC | 1 cut(s) | 33 |
| SaqAI | TTAA | 1 cut(s) | 102 |
| Sau3AI | GATC | 1 cut(s) | 207 |
| SetI | ASST | 4 cut(s) | 78, 102, 174, 244 |
| SfaNI | GCATC | 1 cut(s) | 124 |
| SgeI | CNNG | 8 cut(s) | 56, 60, 78, 89, 150, 204, 223, 234 |
| Sse9I | AATT | 2 cut(s) | 87, 200 |
| SspI | AATATT | 1 cut(s) | 112 |
| TasI | AATT | 2 cut(s) | 87, 200 |
| TfiI | GAWTC | 1 cut(s) | 118 |
| Tru1I | TTAA | 1 cut(s) | 102 |
| Tru9I | TTAA | 1 cut(s) | 102 |
| TspDTI | ATGAA | 1 cut(s) | 252 |
| XapI | RAATTY | 1 cut(s) | 200 |