AT3G01080
WRKY Family

WRKY Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0023623
Physical Location & Seq
Forward (+)
25355 .. 27712
2358 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01080.1

Sequence Viewer

Length: 1272 bp
ATGGCGGTTGAAGACGATGTATCTTTGATAAGAACGACGACGTTAGTGGCACCAACAAGACCCACGATTACAGTTCCTCATAGACCTCCGGCGATCGAAACGGCGGCGTATTTCTTTGGCGGTGGAGATGGGCTTAGTCTAAGCCCAGGGCCACTTTCTTTTGTCTCTTCTTTGTTTGTTGATAACTTCCCTGACGTCTTGACGCCGGATAACCAACGGACGACGTCGTTTACTCATCTTCTCACCTCGCCCATGTTCTTTCCCCCGCAGTCGTCAGCTCATACCGGCTTTATTCAACCACGGCAGCAGTCACAACCGCAACCACAACGACCAGACACGTTTCCTCACCATATGCCACCATCGACATCCGTCGCCGTCCATGGTCGTCAATCTTTAGACGTTTCACAAGTAGATCAAAGAGCTCGAAACCATTATAATAATCCGGGGAATAACAATAATAACCGGTCGTATAACGTTGTGAACGTTGATAAACCGGCGGATGACGGTTATAACTGGAGGAAGTACGGACAAAAGCCTATCAAAGGGTGTGAATATCCAAGGAGTTATTACAAATGTACACATGTTAACTGTCCGGTGAAGAAGAAAGTCGAACGGTCATCGGATGGACAGATCACTCAGATCATTTACAAAGGTCAACATGATCACGAGAGGCCTCAGAATCGCCGTGGCGGTGGAGGCAGAGATTCCACTGAGGTTGGTGGTGCAGGGCAAATGATGGAATCTAGTGATGATAGTGGTTATCGTAAGGATCATGATGATGATGATGATGATGATGAAGATGATGAAGATCTTCCGGCTTCAAAGATAAGAAGAATAGACGGTGTGTCGACGACTCACCGGACGGTGACCGAGCCTAAGATTATCGTTCAGACAAAAAGTGAAGTCGATCTTCTCGACGATGGCTATAGGTGGCGTAAGTACGGACAAAAAGTTGTCAAAGGAAATCCCCATCCAAGGAGCTATTATAAATGTACAACGCCAAATTGTACGGTCCGTAAACATGTAGAGAGAGCTTCCACGGATGCTAAGGCTGTGATTACAACTTACGAAGGTAAACACAATCACGATGTCCCTGCCGCTAGAAACGGTACCGCGGCAGCAACCGCAGCTGCGGTGGGGCCGTCTGACCACCATCGTATGAGATCAATGTCGGGGAACAATATGCAACAACATATGAGTTTCGGTAACAATAATAACACAGGCCAATCTCCGGTTCTTTTGAGGTTGAAAGAAGAGAAAATCACAATTTGA

Protein Analysis

423

Amino Acids

47.13

Weight (kDa)

8.17

Isoelectric Point (pI)

48.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 167 - 223 1.6e-25 WRKY DNA -binding domain
WRKY PF03106 306 - 363 4.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0023623)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G01080 AT3G01080 AT3G01080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 435, 510, 987
AatII GACGTC 2 cut(s) 198, 227
Acc65I GGTACC 1 cut(s) 1109
AccB1I GGYRCC 2 cut(s) 49, 1109
AccI GTMKAC 1 cut(s) 848
AccII CGCG 1 cut(s) 1115
AclI AACGTT 2 cut(s) 474, 483
AclWI GGATC 1 cut(s) 777
AcyI GRCGYC 3 cut(s) 195, 203, 224
AfaI GTAC 6 cut(s) 524, 577, 941, 994, 1009, 1111
AfiI CCNNNNNNNGG 3 cut(s) 542, 975, 1231
AflIII ACRYGT 3 cut(s) 336, 580, 1021
AgeI ACCGGT 1 cut(s) 462
AgsI TTSAA 4 cut(s) 11, 296, 822, 1249
AhdI GACNNNNNGTC 1 cut(s) 844
AjnI CCWGG 1 cut(s) 145
AjuI GAANNNNNNNTTGG 2 cut(s) 1218, 1250
AluBI AGCT 5 cut(s) 278, 422, 981, 1034, 1130
AluI AGCT 5 cut(s) 278, 422, 981, 1034, 1130
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 777
AoxI GGCC 4 cut(s) 149, 671, 1139, 1222
ApeKI GCWGC 4 cut(s) 304, 1118, 1127, 1130
AsiGI ACCGGT 1 cut(s) 462
Asp700I GAANNNNTTC 1 cut(s) 810
Asp718I GGTACC 1 cut(s) 1109
AspS9I GGNCC 3 cut(s) 149, 1012, 1139
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 5 cut(s) 235, 338, 607, 848, 877
AvaII GGWCC 1 cut(s) 1012
BanI GGYRCC 2 cut(s) 49, 1109
BanII GRGCYC 1 cut(s) 424
BauI CACGAG 1 cut(s) 665
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 4 cut(s) 316, 1117, 1130, 1139
BccI CCATC 7 cut(s) 122, 367, 617, 730, 914, 978, 1161
BceAI ACGGC 5 cut(s) 117, 317, 359, 669, 1126
BcgI CGANNNNNNTGC 2 cut(s) 1076, 1110
BciT130I CCWGG 1 cut(s) 147
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 2 cut(s) 744, 1101
BfmI CTRYAG 1 cut(s) 925
BglII AGATCT 1 cut(s) 808
BisI GCNGC 7 cut(s) 105, 305, 1098, 1116, 1119, 1128, 1131
BlsI GCNGC 7 cut(s) 106, 306, 1099, 1117, 1120, 1129, 1132
Bme1390I CCNGG 2 cut(s) 147, 444
Bme18I GGWCC 1 cut(s) 1012
BmeRI GACNNNNNGTC 1 cut(s) 844
BmgT120I GGNCC 3 cut(s) 149, 1012, 1139
BmiI GGNNCC 3 cut(s) 51, 1111, 1140
BmrFI CCNGG 2 cut(s) 147, 444
BmsI GCATC 1 cut(s) 1033
BoxI GACNNNNGTC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 535
Bpu10I CCTNAGC 1 cut(s) 1047
BpuMI CCSGG 1 cut(s) 444
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 3 cut(s) 195, 203, 224
BsaWI WCCGGW 4 cut(s) 462, 592, 858, 1231
Bsc4I CCNNNNNNNGG 3 cut(s) 542, 975, 1231
Bse118I RCCGGY 3 cut(s) 284, 462, 493
Bse1I ACTGG 1 cut(s) 518
Bse8I GATNNNNATC 1 cut(s) 807
BseBI CCWGG 1 cut(s) 147
BseGI GGATG 5 cut(s) 365, 505, 628, 970, 1048
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 3 cut(s) 542, 975, 1231
BseMII CTCAG 3 cut(s) 650, 689, 702
BseNI ACTGG 1 cut(s) 518
BseXI GCAGC 4 cut(s) 316, 1117, 1130, 1139
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 1115
Bsh1285I CGRYCG 2 cut(s) 96, 467
BshFI GGCC 4 cut(s) 151, 673, 1141, 1224
BshNI GGYRCC 2 cut(s) 49, 1109
BshTI ACCGGT 1 cut(s) 462
BsiEI CGRYCG 2 cut(s) 96, 467
BsiHKAI GWGCWC 1 cut(s) 424
BslFI GGGAC 1 cut(s) 1076
BslI CCNNNNNNNGG 3 cut(s) 542, 975, 1231
BsmAI GTCTC 1 cut(s) 169
BsmFI GGGAC 1 cut(s) 1076
BsnI GGCC 4 cut(s) 151, 673, 1141, 1224
Bsp1286I GDGCHC 1 cut(s) 424
Bsp1407I TGTACA 2 cut(s) 575, 992
Bsp143I GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
Bsp19I CCATGG 1 cut(s) 379
BspANI GGCC 4 cut(s) 151, 673, 1141, 1224
BspCNI CTCAG 3 cut(s) 649, 688, 703
BspFNI CGCG 1 cut(s) 1115
BspHI TCATGA 1 cut(s) 772
BspLI GGNNCC 3 cut(s) 51, 1111, 1140
BspPI GGATC 1 cut(s) 777
BspT107I GGYRCC 2 cut(s) 49, 1109
BsrFI RCCGGY 3 cut(s) 284, 462, 493
BsrGI TGTACA 2 cut(s) 575, 992
BsrI ACTGG 1 cut(s) 518
BssAI RCCGGY 3 cut(s) 284, 462, 493
BssMI GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
BssNI GRCGYC 3 cut(s) 195, 203, 224
BssSI CACGAG 1 cut(s) 665
BssT1I CCWWGG 3 cut(s) 379, 557, 974
Bst2BI CACGAG 1 cut(s) 665
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 8 cut(s) 73, 506, 590, 615, 842, 865, 1012, 1109
Bst6I CTCTTC 2 cut(s) 172, 1248
BstACI GRCGYC 3 cut(s) 195, 203, 224
BstAUI TGTACA 2 cut(s) 575, 992
BstDEI CTNAG 7 cut(s) 134, 140, 636, 675, 711, 876, 1047
BstDSI CCRYGG 5 cut(s) 299, 379, 685, 1038, 1113
BstEII GGTNACC 1 cut(s) 865
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 5 cut(s) 365, 505, 628, 970, 1048
BstFNI CGCG 1 cut(s) 1115
BstKTI GATC 9 cut(s) 96, 415, 633, 642, 664, 772, 811, 910, 1166
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
BstMCI CGRYCG 2 cut(s) 96, 467
BstMWI GCNNNNNNNGC 3 cut(s) 696, 1124, 1127
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 2 cut(s) 584, 1025
BstPAI GACNNNNGTC 1 cut(s) 368
BstPI GGTNACC 1 cut(s) 865
BstSCI CCNGG 2 cut(s) 145, 442
BstSFI CTRYAG 1 cut(s) 925
BstUI CGCG 1 cut(s) 1115
BstV1I GCAGC 4 cut(s) 316, 1117, 1130, 1139
BstV2I GAAGAC 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
BsuRI GGCC 4 cut(s) 151, 673, 1141, 1224
BtgI CCRYGG 5 cut(s) 299, 379, 685, 1038, 1113
BtsCI GGATG 5 cut(s) 365, 505, 628, 970, 1048
BtsIMutI CAGTG 1 cut(s) 708
CciI TCATGA 1 cut(s) 772
Cfr10I RCCGGY 3 cut(s) 284, 462, 493
Cfr13I GGNCC 3 cut(s) 149, 1012, 1139
Cfr42I CCGCGG 1 cut(s) 1116
CpoI CGGWCCG 1 cut(s) 1012
CseI GACGC 1 cut(s) 211
Csp6I GTAC 6 cut(s) 523, 576, 940, 993, 1008, 1110
CspAI ACCGGT 1 cut(s) 462
CspCI CAANNNNNGTGG 2 cut(s) 697, 732
CspI CGGWCCG 1 cut(s) 1012
CviAII CATG 6 cut(s) 253, 380, 581, 659, 773, 1022
CviQI GTAC 6 cut(s) 523, 576, 940, 993, 1008, 1110
DdeI CTNAG 7 cut(s) 134, 140, 636, 675, 711, 876, 1047
DpnI GATC 9 cut(s) 95, 414, 632, 641, 663, 771, 810, 909, 1165
DpnII GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
DriI GACNNNNNGTC 1 cut(s) 844
Eam1104I CTCTTC 2 cut(s) 172, 1248
Eam1105I GACNNNNNGTC 1 cut(s) 844
EarI CTCTTC 2 cut(s) 172, 1248
EciI GGCGGA 1 cut(s) 512
Ecl136II GAGCTC 1 cut(s) 422
Eco130I CCWWGG 3 cut(s) 379, 557, 974
Eco147I AGGCCT 1 cut(s) 673
Eco24I GRGCYC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 1012
Eco53kI GAGCTC 1 cut(s) 422
Eco91I GGTNACC 1 cut(s) 865
EcoICRI GAGCTC 1 cut(s) 422
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO65I GGTNACC 1 cut(s) 865
EcoRII CCWGG 1 cut(s) 145
EcoT14I CCWWGG 3 cut(s) 379, 557, 974
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 3 cut(s) 379, 557, 974
FaeI CATG 6 cut(s) 256, 383, 584, 662, 776, 1025
FalI AAGNNNNNCTT 2 cut(s) 894, 926
FaqI GGGAC 1 cut(s) 1076
FatI CATG 6 cut(s) 252, 379, 580, 658, 772, 1021
FauI CCCGC 1 cut(s) 273
FauNDI CATATG 2 cut(s) 351, 1194
FbaI TGATCA 1 cut(s) 661
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 7 cut(s) 105, 305, 1098, 1116, 1119, 1128, 1131
FokI GGATG 5 cut(s) 352, 512, 635, 957, 1055
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 7 cut(s) 105, 305, 1098, 1116, 1119, 1128, 1131
FspBI CTAG 2 cut(s) 744, 1101
GluI GCNGC 7 cut(s) 105, 305, 1098, 1116, 1119, 1128, 1131
GsuI CTGGAG 1 cut(s) 535
HaeIII GGCC 4 cut(s) 151, 673, 1141, 1224
HgaI GACGC 1 cut(s) 211
Hin1I GRCGYC 3 cut(s) 195, 203, 224
Hin1II CATG 6 cut(s) 256, 383, 584, 662, 776, 1025
HincII GTYRAC 3 cut(s) 586, 656, 849
HindII GTYRAC 3 cut(s) 586, 656, 849
HinfI GANTC 4 cut(s) 679, 704, 740, 853
HpaI GTTAAC 1 cut(s) 586
HphI GGTGA 5 cut(s) 235, 338, 607, 848, 877
Hpy166II GTNNAC 8 cut(s) 231, 481, 578, 586, 656, 849, 1019, 1076
Hpy188I TCNGA 5 cut(s) 622, 639, 678, 891, 1147
Hpy188III TCNNGA 5 cut(s) 199, 665, 773, 914, 1085
Hpy8I GTNNAC 8 cut(s) 231, 481, 578, 586, 656, 849, 1019, 1076
Hpy99I CGWCG 7 cut(s) 40, 43, 226, 229, 374, 853, 920
HpyAV CCTTC 1 cut(s) 1064
HpyCH4III ACNGT 8 cut(s) 73, 506, 590, 615, 842, 865, 1012, 1109
HpyCH4IV ACGT 7 cut(s) 41, 195, 224, 338, 399, 474, 483
HpyCH4V TGCA 2 cut(s) 725, 1186
HpyF10VI GCNNNNNNNGC 3 cut(s) 696, 1124, 1127
HpyF3I CTNAG 7 cut(s) 134, 140, 636, 675, 711, 876, 1047
HpySE526I ACGT 7 cut(s) 41, 195, 224, 338, 399, 474, 483
Hsp92I GRCGYC 3 cut(s) 195, 203, 224
Hsp92II CATG 6 cut(s) 256, 383, 584, 662, 776, 1025
KpnI GGTACC 1 cut(s) 1113
Ksp22I TGATCA 1 cut(s) 661
KspAI GTTAAC 1 cut(s) 586
KspI CCGCGG 1 cut(s) 1116
Kzo9I GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
LmnI GCTCC 1 cut(s) 978
Lsp1109I GCAGC 4 cut(s) 316, 1117, 1130, 1139
LweI GCATC 1 cut(s) 1033
MaeI CTAG 2 cut(s) 744, 1101
MaeII ACGT 7 cut(s) 41, 195, 224, 338, 399, 474, 483
MaeIII GTNAC 3 cut(s) 309, 865, 1205
MalI GATC 9 cut(s) 95, 414, 632, 641, 663, 771, 810, 909, 1165
MboI GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 2 cut(s) 1003, 1266
MlyI GAGTC 1 cut(s) 847
MroXI GAANNNNTTC 1 cut(s) 810
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 777
MspA1I CMGCKG 2 cut(s) 1115, 1130
MspR9I CCNGG 2 cut(s) 147, 444
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 1 cut(s) 1115
MwoI GCNNNNNNNGC 3 cut(s) 696, 1124, 1127
NciI CCSGG 1 cut(s) 444
NcoI CCATGG 1 cut(s) 379
NdeI CATATG 2 cut(s) 351, 1194
NdeII GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
NlaIII CATG 6 cut(s) 256, 383, 584, 662, 776, 1025
NlaIV GGNNCC 3 cut(s) 51, 1111, 1140
NmuCI GTSAC 2 cut(s) 309, 865
NspI RCATGY 2 cut(s) 584, 1025
PagI TCATGA 1 cut(s) 772
PasI CCCWGGG 1 cut(s) 146
PceI AGGCCT 1 cut(s) 673
PciI ACATGT 2 cut(s) 580, 1021
PcsI WCGNNNNNNNCGW 2 cut(s) 480, 912
PdmI GAANNNNTTC 1 cut(s) 810
PfeI GAWTC 3 cut(s) 679, 704, 740
PflFI GACNNNGTC 1 cut(s) 223
PinAI ACCGGT 1 cut(s) 462
PkrI GCNGC 7 cut(s) 106, 306, 1099, 1117, 1120, 1129, 1132
Ple19I CGATCG 1 cut(s) 96
PleI GAGTC 1 cut(s) 847
PpsI GAGTC 1 cut(s) 847
PscI ACATGT 2 cut(s) 580, 1021
PshAI GACNNNNGTC 1 cut(s) 368
PsiI TTATAA 3 cut(s) 435, 510, 987
Psp124BI GAGCTC 1 cut(s) 424
Psp1406I AACGTT 2 cut(s) 474, 483
Psp6I CCWGG 1 cut(s) 145
PspEI GGTNACC 1 cut(s) 865
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 3 cut(s) 51, 1111, 1140
PspPI GGNCC 3 cut(s) 149, 1012, 1139
PsuI RGATCY 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 223
PvuI CGATCG 1 cut(s) 96
PvuII CAGCTG 1 cut(s) 1130
RsaI GTAC 6 cut(s) 524, 577, 941, 994, 1009, 1111
RsaNI GTAC 6 cut(s) 523, 576, 940, 993, 1008, 1110
RseI CAYNNNNRTG 1 cut(s) 777
Rsr2I CGGWCCG 1 cut(s) 1012
RsrII CGGWCCG 1 cut(s) 1012
SacI GAGCTC 1 cut(s) 424
SacII CCGCGG 1 cut(s) 1116
SalI GTCGAC 1 cut(s) 847
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 7 cut(s) 105, 305, 1098, 1116, 1119, 1128, 1131
Sau3AI GATC 9 cut(s) 93, 412, 630, 639, 661, 769, 808, 907, 1163
Sau96I GGNCC 3 cut(s) 149, 1012, 1139
SchI GAGTC 1 cut(s) 847
ScrFI CCNGG 2 cut(s) 147, 444
SduI GDGCHC 1 cut(s) 424
SfaNI GCATC 1 cut(s) 1033
SfcI CTRYAG 1 cut(s) 925
Sfr303I CCGCGG 1 cut(s) 1116
SgrBI CCGCGG 1 cut(s) 1116
SinI GGWCC 1 cut(s) 1012
SmiMI CAYNNNNRTG 1 cut(s) 777
Sse9I AATT 2 cut(s) 1003, 1266
SseBI AGGCCT 1 cut(s) 673
SspMI CTAG 2 cut(s) 744, 1101
SstI GAGCTC 1 cut(s) 424
StuI AGGCCT 1 cut(s) 673
StyD4I CCNGG 2 cut(s) 145, 442
StyI CCWWGG 3 cut(s) 379, 557, 974
TaaI ACNGT 8 cut(s) 73, 506, 590, 615, 842, 865, 1012, 1109
TaiI ACGT 7 cut(s) 44, 198, 227, 341, 402, 477, 486
TaqI TCGA 7 cut(s) 96, 362, 424, 609, 848, 906, 915
TaqII GACCGA 1 cut(s) 884
TasI AATT 2 cut(s) 1003, 1266
TatI WGTACW 2 cut(s) 575, 992
TauI GCSGC 3 cut(s) 107, 1100, 1118
TfiI GAWTC 3 cut(s) 679, 704, 740
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 2 cut(s) 309, 865
TseI GCWGC 4 cut(s) 304, 1118, 1127, 1130
Tsp45I GTSAC 2 cut(s) 309, 865
TspDTI ATGAA 2 cut(s) 810, 819
TspGWI ACGGA 6 cut(s) 232, 358, 540, 957, 1004, 1055
TspRI CASTG 1 cut(s) 715
Tth111I GACNNNGTC 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 1012
XagI CCTNNNNNAGG 1 cut(s) 540
XceI RCATGY 2 cut(s) 584, 1025
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 1 cut(s) 810
XspI CTAG 2 cut(s) 744, 1101
ZraI GACGTC 2 cut(s) 196, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.