AT3G10960

permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0014608
Physical Location & Seq
Reverse (-)
3424813 .. 3427058
2246 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10960.1

Sequence Viewer

Length: 1740 bp
ATGGAGCAACAGCAACAACAACAACTACCTTCTACTACTACTAGACCCAAGCCCAAGCTACTAAACCGGCTCAATACCTACGTCGGTTCGAGCCGGGTTGGCAAACGGTTCAAACTCGCCGAACGTAATTCAACTTTCACGACGGAGCTCCGTGCTGGTACCGCCACTTTCTTAACCATGGCTTATATCCTCGCCGTCAACGCTTCAATCCTTTCTGATTCCGGTGGTACTTGCTCTGTTTCCGATTGTATCCCTCTATGCTCAAACCCAGCGATCGAACCTTCGCAGTGTACCGGACCGGGTCTCCGATTAATCCAACCCGACGTTTCCTGTAAATTCAACCCGGTTAATCCTGGTTACGCCGCATGCGTTGAAGAAATTCGAAAGGATCTAATCGTCGCCACCGTAGCTGCGTCACTAATCGGTTGTGTAATCATGGGATTGATGGCGAATCTTCCTCTAGCTTTAGCTCCGGGAATGGGAACTAACGCTTATTTCGCTTACACCGTCGTTGGATTTCACGGATCCGGTTCAATCTCATACCGGACCGCACTCGCCGCCGTTTTCATCGAAGGACTGATATTTCTCTTCATCTCCGCCATAGGTTTCAGAGCAAAACTTGCGAAACTCGTTCCTAAACCGGTCAGAATCAGTTCCTCCGCCGGAATCGGACTTTTCCTCGCTTTCATCGGTTTACAGAACAATCAAGGAATCGGTTTAGTCGGTTATAGTCCTTCAACACTAGTCACTCTCGCCGCTTGTCCAGCCTCATCACGAATATCGTTAGCTCCCGTTATAACGTCAGCTAACGGAACCGTTAGTTTACTCGCCGGAGGATCAGTTTCCGGCGATATTATGTGTATTCACGGACGAATGGAGAGTCCAACGTTCTGGTTAGGTATCGTCGGATTCGTGATCATAGCTTATTGTTTGGTAAAGAACGTGAAAGGAGCGATGATCTACGGGATCGTCTTCGTAACGGCCGTTTCGTGGTTCCGTAACACAGAGGTAACGGCGTTTCCGAACACATCGGCCGGAGATGCAGCTCATGATTATTTCAAAAAGATCGTTGATGTACACGTCATTAAACATACCGCCGGAGCTTTAAGCTTCTCCGGGATAAACAAAGGACATTTCTGGGAAGCTCTGGTGACATTCCTCTACGTTGATATTTTGGATACGACGGGAACGCTTTACTCCATGGCTAGATTCGCCGGATTCGTCGACGAGAAAGGAGATTTCGCCGGACAGTATTTCGCGTTTATGTCTGACGCGTCTGCGATTGTGATCGGATCGTTGCTTGGTACATCTCCGGTGACTGTTTTCATCGAGTCGTCGACGGGGATAAGAGAAGGAGGGAGGACGGGTCTAACGGCGATTACGGTGGCTGTTTATTTCTTGTTAGCGATGTTTTTCACACCGTTGCTAGCTTCGATTCCCGCATGGGCGGTTGGTCCGCCGTTGATATTAGTCGGAGTGATGATGATGAAATCAGTCACAGAGATTGATTGGGAAGATATGAGAGAGGCGATTCCGGCATTTGTGACAATGATTCTGATGCCGTTGACTTACTCCGTCGCGTATGGATTGATCGGAGGGATTGGGAGCTATGTAGTGTTGCATCTATGGGATTGGGGAGAAGAAGGTTTGGTGAAATTAGGGTTTTTGAAAAGGAAAGTTAAAGAAGAGGATAATAATAATGGAGTTGTCAAAGCTAGTGAGATTGATACTACCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005345 GO:0006810 GO:0006863 GO:0008150 GO:0015205 GO:0015851 GO:0015853 GO:0015854 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0072530 GO:1904823
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Xan_ur_permease

Protein Analysis

579

Amino Acids

61.67

Weight (kDa)

7.96

Isoelectric Point (pI)

30.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Xan_ur_permease PF00860 124 - 240 2.2e-12 Permease family
Xan_ur_permease PF00860 277 - 529 1.7e-18 Permease family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014608)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10960
fragaria_vesca FvH4_7g25660
malus_domestica MD01G1163900.v1.1 MD07G1231600.v1.1
prunus_persica Prupe.2G261700_v2.0.a1
pyrus_communis pycom01g17830 pycom07g20940
rosa_chinensis RchiOBHm_Chr1g0371841
rosa_laevigata RLG00000026939
rosa_multiflora Rmu_sc0001755.1_g000048
rosa_roxburghii Rroxscaffold_4G00285410
rosa_rugosa Rorug01G0366700
rosa_samantha Rh1AG376700 Rh1BG340000 Rh1CG353000 Rh1DG371000
rosa_wichuraiana Rw1G033140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 797
Acc65I GGTACC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 158
AccI GTMKAC 2 cut(s) 1224, 1337
AccII CGCG 3 cut(s) 1259, 1274, 1580
AclI AACGTT 1 cut(s) 887
AclWI GGATC 6 cut(s) 396, 519, 532, 844, 974, 1300
AcoI YGGCCR 2 cut(s) 981, 1032
AcsI RAATTY 2 cut(s) 335, 378
AfaI GTAC 5 cut(s) 160, 229, 292, 1077, 1306
AfiI CCNNNNNNNGG 2 cut(s) 479, 990
AflIII ACRYGT 2 cut(s) 1078, 1272
AgeI ACCGGT 1 cut(s) 640
AgsI TTSAA 9 cut(s) 112, 132, 207, 340, 374, 534, 738, 1060, 1669
AhlI ACTAGT 1 cut(s) 742
AjiI CACGTC 1 cut(s) 1081
AjnI CCWGG 1 cut(s) 352
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 6 cut(s) 396, 519, 532, 844, 974, 1300
AoxI GGCC 2 cut(s) 981, 1032
ApeKI GCWGC 2 cut(s) 410, 1043
ApoI RAATTY 2 cut(s) 335, 378
ArsI GACNNNNNNTTYG 2 cut(s) 865, 897
AseI ATTAAT 1 cut(s) 311
AsiGI ACCGGT 1 cut(s) 640
Asp700I GAANNNNTTC 2 cut(s) 378, 652
Asp718I GGTACC 1 cut(s) 158
AspS9I GGNCC 3 cut(s) 296, 546, 1454
AsuC2I CCSGG 5 cut(s) 95, 300, 344, 474, 1117
AsuHPI GGTGA 3 cut(s) 1162, 1327, 1663
AsuII TTCGAA 1 cut(s) 382
AsuNHI GCTAGC 1 cut(s) 1426
AvaII GGWCC 3 cut(s) 296, 546, 1454
BaeI ACNNNNGTAYC 1 cut(s) 1719
BamHI GGATCC 1 cut(s) 524
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 964
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 397, 1055
BccI CCATC 1 cut(s) 439
BceAI ACGGC 8 cut(s) 179, 545, 968, 996, 1029, 1389, 1444, 1546
BciT130I CCWGG 1 cut(s) 354
BciVI GTATCC 2 cut(s) 260, 1171
BclI TGATCA 1 cut(s) 915
BcnI CCSGG 5 cut(s) 95, 300, 344, 474, 1117
BcoDI GTCTC 1 cut(s) 308
BcuI ACTAGT 1 cut(s) 742
BfaI CTAG 6 cut(s) 42, 461, 743, 1206, 1427, 1716
BfuI GTATCC 2 cut(s) 260, 1171
BglI GCCNNNNNGGC 1 cut(s) 99
BisI GCNGC 5 cut(s) 363, 411, 558, 756, 1044
BlsI GCNGC 5 cut(s) 364, 412, 559, 757, 1045
Bme1390I CCNGG 6 cut(s) 95, 300, 344, 354, 474, 1117
Bme18I GGWCC 3 cut(s) 296, 546, 1454
BmgBI CACGTC 1 cut(s) 1081
BmgT120I GGNCC 3 cut(s) 296, 546, 1454
BmiI GGNNCC 4 cut(s) 160, 526, 814, 995
BmrFI CCNGG 6 cut(s) 95, 300, 344, 354, 474, 1117
BmsI GCATC 3 cut(s) 1030, 1548, 1630
BmtI GCTAGC 1 cut(s) 1430
BpiI GAAGAC 1 cut(s) 964
Bpu14I TTCGAA 1 cut(s) 382
BpuMI CCSGG 5 cut(s) 95, 300, 344, 474, 1117
BsaBI GATNNNNATC 1 cut(s) 1286
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 2 cut(s) 177, 1200
BsaWI WCCGGW 6 cut(s) 221, 293, 527, 543, 640, 1312
BsaXI ACNNNNNCTCC 6 cut(s) 288, 318, 825, 855, 1351, 1381
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 990
Bse118I RCCGGY 2 cut(s) 66, 640
Bse8I GATNNNNATC 1 cut(s) 1286
BseBI CCWGG 1 cut(s) 354
BseDI CCNNGG 2 cut(s) 177, 1200
BseJI GATNNNNATC 1 cut(s) 1286
BseLI CCNNNNNNNGG 2 cut(s) 479, 990
BseX3I CGGCCG 2 cut(s) 981, 1032
BseXI GCAGC 2 cut(s) 397, 1055
BseYI CCCAGC 1 cut(s) 268
Bsh1236I CGCG 3 cut(s) 1259, 1274, 1580
Bsh1285I CGRYCG 3 cut(s) 276, 984, 1035
BshFI GGCC 2 cut(s) 983, 1034
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 640
BsiEI CGRYCG 3 cut(s) 276, 984, 1035
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 2 cut(s) 479, 990
BsmAI GTCTC 1 cut(s) 308
BsnI GGCC 2 cut(s) 983, 1034
Bso31I GGTCTC 1 cut(s) 308
Bsp119I TTCGAA 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 150
Bsp1407I TGTACA 1 cut(s) 1075
Bsp19I CCATGG 2 cut(s) 177, 1200
BspANI GGCC 2 cut(s) 983, 1034
BspFNI CGCG 3 cut(s) 1259, 1274, 1580
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 4 cut(s) 160, 526, 814, 995
BspOI GCTAGC 1 cut(s) 1430
BspPI GGATC 6 cut(s) 396, 519, 532, 844, 974, 1300
BspT104I TTCGAA 1 cut(s) 382
BspT107I GGYRCC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 308
BsrFI RCCGGY 2 cut(s) 66, 640
BsrGI TGTACA 1 cut(s) 1075
BssAI RCCGGY 2 cut(s) 66, 640
BssECI CCNNGG 2 cut(s) 177, 1200
BssT1I CCWWGG 2 cut(s) 177, 1200
Bst2UI CCWGG 1 cut(s) 354
Bst4CI ACNGT 9 cut(s) 108, 406, 508, 817, 1251, 1321, 1384, 1422, 1735
Bst6I CTCTTC 2 cut(s) 593, 1680
BstAPI GCANNNNNTGC 1 cut(s) 620
BstAUI TGTACA 1 cut(s) 1075
BstBI TTCGAA 1 cut(s) 382
BstC8I GCNNGC 2 cut(s) 367, 1428
BstDSI CCRYGG 2 cut(s) 177, 1200
BstFNI CGCG 3 cut(s) 1259, 1274, 1580
BstMAI GTCTC 1 cut(s) 308
BstMCI CGRYCG 3 cut(s) 276, 984, 1035
BstNI CCWGG 1 cut(s) 354
BstNSI RCATGY 1 cut(s) 369
BstSCI CCNGG 6 cut(s) 93, 298, 342, 352, 472, 1115
BstUI CGCG 3 cut(s) 1259, 1274, 1580
BstV1I GCAGC 2 cut(s) 397, 1055
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 2 cut(s) 388, 524
BstXI CCANNNNNNTGG 1 cut(s) 891
BstYI RGATCY 2 cut(s) 388, 524
BstZI CGGCCG 2 cut(s) 981, 1032
BsuI GTATCC 2 cut(s) 260, 1171
BsuRI GGCC 2 cut(s) 983, 1034
BtgI CCRYGG 2 cut(s) 177, 1200
BtgZI GCGATG 2 cut(s) 968, 1421
BtrI CACGTC 1 cut(s) 1081
BtsI GCAGTG 1 cut(s) 293
BtsIMutI CAGTG 1 cut(s) 293
Cac8I GCNNGC 2 cut(s) 367, 1428
CciI TCATGA 1 cut(s) 1048
Cfr10I RCCGGY 2 cut(s) 66, 640
Cfr13I GGNCC 3 cut(s) 296, 546, 1454
CpoI CGGWCCG 2 cut(s) 296, 546
CseI GACGC 3 cut(s) 402, 1263, 1280
Csp6I GTAC 5 cut(s) 159, 228, 291, 1076, 1305
CspAI ACCGGT 1 cut(s) 640
CspI CGGWCCG 2 cut(s) 296, 546
CviAII CATG 6 cut(s) 178, 366, 436, 1049, 1201, 1443
CviQI GTAC 5 cut(s) 159, 228, 291, 1076, 1305
EaeI YGGCCR 2 cut(s) 981, 1032
EagI CGGCCG 2 cut(s) 981, 1032
Eam1104I CTCTTC 2 cut(s) 593, 1680
EarI CTCTTC 2 cut(s) 593, 1680
EciI GGCGGA 3 cut(s) 586, 649, 1446
Ecl136II GAGCTC 1 cut(s) 148
EclXI CGGCCG 2 cut(s) 981, 1032
Eco130I CCWWGG 2 cut(s) 177, 1200
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 308
Eco47I GGWCC 3 cut(s) 296, 546, 1454
Eco52I CGGCCG 2 cut(s) 981, 1032
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 352
EcoT14I CCWWGG 2 cut(s) 177, 1200
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 177, 1200
FaeI CATG 6 cut(s) 181, 369, 439, 1052, 1204, 1446
FatI CATG 6 cut(s) 177, 365, 435, 1048, 1200, 1442
FauI CCCGC 1 cut(s) 1447
FbaI TGATCA 1 cut(s) 915
FblI GTMKAC 2 cut(s) 1224, 1337
Fnu4HI GCNGC 5 cut(s) 363, 411, 558, 756, 1044
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 5 cut(s) 363, 411, 558, 756, 1044
FspBI CTAG 6 cut(s) 42, 461, 743, 1206, 1427, 1716
GluI GCNGC 5 cut(s) 363, 411, 558, 756, 1044
GsaI CCCAGC 1 cut(s) 272
HaeIII GGCC 2 cut(s) 983, 1034
HgaI GACGC 3 cut(s) 402, 1263, 1280
Hin1II CATG 6 cut(s) 181, 369, 439, 1052, 1204, 1446
HincII GTYRAC 4 cut(s) 199, 1225, 1338, 1566
HindII GTYRAC 4 cut(s) 199, 1225, 1338, 1566
HindIII AAGCTT 1 cut(s) 1108
HphI GGTGA 3 cut(s) 1162, 1327, 1663
Hpy166II GTNNAC 8 cut(s) 199, 291, 695, 824, 1078, 1225, 1338, 1566
Hpy188III TCNNGA 4 cut(s) 139, 774, 913, 1049
Hpy8I GTNNAC 8 cut(s) 199, 291, 695, 824, 1078, 1225, 1338, 1566
HpyAV CCTTC 6 cut(s) 39, 291, 566, 744, 1346, 1637
HpyCH4III ACNGT 9 cut(s) 108, 406, 508, 817, 1251, 1321, 1384, 1422, 1735
HpyCH4IV ACGT 8 cut(s) 81, 124, 324, 800, 887, 942, 1080, 1164
HpyCH4V TGCA 2 cut(s) 1043, 1621
HpySE526I ACGT 8 cut(s) 81, 124, 324, 800, 887, 942, 1080, 1164
Hsp92II CATG 6 cut(s) 181, 369, 439, 1052, 1204, 1446
KpnI GGTACC 1 cut(s) 162
Ksp22I TGATCA 1 cut(s) 915
LmnI GCTCC 8 cut(s) 4, 145, 153, 475, 793, 950, 1100, 1605
Lsp1109I GCAGC 2 cut(s) 397, 1055
LweI GCATC 3 cut(s) 1030, 1548, 1630
MaeI CTAG 6 cut(s) 42, 461, 743, 1206, 1427, 1716
MaeII ACGT 8 cut(s) 81, 124, 324, 800, 887, 942, 1080, 1164
MboII GAAGA 7 cut(s) 386, 446, 580, 964, 1526, 1652, 1697
MflI RGATCY 2 cut(s) 388, 524
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 4 cut(s) 127, 335, 378, 1655
MluI ACGCGT 1 cut(s) 1272
MlyI GAGTC 2 cut(s) 889, 1340
MmeI TCCRAC 5 cut(s) 340, 493, 886, 908, 1453
MroXI GAANNNNTTC 2 cut(s) 378, 652
MseI TTAA 6 cut(s) 173, 311, 348, 1086, 1106, 1680
MspR9I CCNGG 6 cut(s) 95, 300, 344, 354, 474, 1117
MvaI CCWGG 1 cut(s) 354
MvnI CGCG 3 cut(s) 1259, 1274, 1580
NciI CCSGG 5 cut(s) 95, 300, 344, 474, 1117
NcoI CCATGG 2 cut(s) 177, 1200
NheI GCTAGC 1 cut(s) 1426
NlaIII CATG 6 cut(s) 181, 369, 439, 1052, 1204, 1446
NlaIV GGNNCC 4 cut(s) 160, 526, 814, 995
NmuCI GTSAC 6 cut(s) 414, 745, 1150, 1315, 1495, 1543
NspI RCATGY 1 cut(s) 369
NspV TTCGAA 1 cut(s) 382
PaeI GCATGC 1 cut(s) 369
PagI TCATGA 1 cut(s) 1048
PcsI WCGNNNNNNNCGW 8 cut(s) 366, 402, 504, 909, 981, 986, 1019, 1218
PdmI GAANNNNTTC 2 cut(s) 378, 652
PflFI GACNNNGTC 1 cut(s) 300
PfoI TCCNGGA 2 cut(s) 472, 1115
PinAI ACCGGT 1 cut(s) 640
PkrI GCNGC 5 cut(s) 364, 412, 559, 757, 1045
Ple19I CGATCG 1 cut(s) 276
PleI GAGTC 2 cut(s) 888, 1339
PpsI GAGTC 2 cut(s) 888, 1339
PshBI ATTAAT 1 cut(s) 311
PsiI TTATAA 1 cut(s) 797
Psp124BI GAGCTC 1 cut(s) 150
Psp1406I AACGTT 1 cut(s) 887
Psp6I CCWGG 1 cut(s) 352
PspFI CCCAGC 1 cut(s) 268
PspGI CCWGG 1 cut(s) 352
PspN4I GGNNCC 4 cut(s) 160, 526, 814, 995
PspPI GGNCC 3 cut(s) 296, 546, 1454
PsuI RGATCY 2 cut(s) 388, 524
PsyI GACNNNGTC 1 cut(s) 300
PvuI CGATCG 1 cut(s) 276
RsaI GTAC 5 cut(s) 160, 229, 292, 1077, 1306
RsaNI GTAC 5 cut(s) 159, 228, 291, 1076, 1305
Rsr2I CGGWCCG 2 cut(s) 296, 546
RsrII CGGWCCG 2 cut(s) 296, 546
SacI GAGCTC 1 cut(s) 150
SalI GTCGAC 2 cut(s) 1223, 1336
SaqAI TTAA 6 cut(s) 173, 311, 348, 1086, 1106, 1680
SatI GCNGC 5 cut(s) 363, 411, 558, 756, 1044
Sau96I GGNCC 3 cut(s) 296, 546, 1454
SchI GAGTC 2 cut(s) 889, 1340
ScrFI CCNGG 6 cut(s) 95, 300, 344, 354, 474, 1117
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 3 cut(s) 1030, 1548, 1630
SfuI TTCGAA 1 cut(s) 382
SgrDI CGTCGACG 2 cut(s) 1223, 1336
SinI GGWCC 3 cut(s) 296, 546, 1454
SpeI ACTAGT 1 cut(s) 742
SphI GCATGC 1 cut(s) 369
Sse9I AATT 4 cut(s) 127, 335, 378, 1655
SspMI CTAG 6 cut(s) 42, 461, 743, 1206, 1427, 1716
SstI GAGCTC 1 cut(s) 150
StyD4I CCNGG 6 cut(s) 93, 298, 342, 352, 472, 1115
StyI CCWWGG 2 cut(s) 177, 1200
TaaI ACNGT 9 cut(s) 108, 406, 508, 817, 1251, 1321, 1384, 1422, 1735
TaiI ACGT 8 cut(s) 84, 127, 327, 803, 890, 945, 1083, 1167
TaqI TCGA 8 cut(s) 89, 276, 382, 570, 1224, 1329, 1337, 1433
TasI AATT 4 cut(s) 127, 335, 378, 1655
TatI WGTACW 1 cut(s) 1075
TauI GCSGC 3 cut(s) 365, 560, 758
Tru1I TTAA 6 cut(s) 173, 311, 348, 1086, 1106, 1680
Tru9I TTAA 6 cut(s) 173, 311, 348, 1086, 1106, 1680
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 6 cut(s) 414, 745, 1150, 1315, 1495, 1543
TseI GCWGC 2 cut(s) 410, 1043
Tsp45I GTSAC 6 cut(s) 414, 745, 1150, 1315, 1495, 1543
TspDTI ATGAA 5 cut(s) 556, 580, 676, 1315, 1502
TspGWI ACGGA 7 cut(s) 140, 158, 537, 825, 882, 986, 1564
TspRI CASTG 1 cut(s) 293
Tth111I GACNNNGTC 1 cut(s) 300
VpaK11BI GGWCC 3 cut(s) 296, 546, 1454
VspI ATTAAT 1 cut(s) 311
XapI RAATTY 2 cut(s) 335, 378
XceI RCATGY 1 cut(s) 369
XmiI GTMKAC 2 cut(s) 1224, 1337
XmnI GAANNNNTTC 2 cut(s) 378, 652
XspI CTAG 6 cut(s) 42, 461, 743, 1206, 1427, 1716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.