AT4G02340

epoxide hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016168
Physical Location & Seq
Forward (+)
1035596 .. 1037615
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02340.1

Sequence Viewer

Length: 975 bp
ATGGAGAAGATAGAGCACACGACGATATCTACCAATGGTATTAACATGCATGTGGCTTCGATTGGTTCAGGTCCAGTGATCCTATTCGTCCATGGCTTTCCCGATCTCTGGTACTCATGGCGTCACCAGCTTGTCTCTTTCGCTGCCTTAGGTTACCGCGCAATCGCTCCAGATCTTCGAGGATACGGCGATTCCGATGCGCCGCCGTCTCGTGAATCTTACACCATCCTTCATATCGTCGGAGACCTCGTCGGATTACTGGATTCGCTCGGTGTTGATCGGGTTTTCCTCGTCGGTCACGATTGGGGAGCAATTGTTGCGTGGTGGCTTTGTATGATCAGGCCTGATCGAGTCAACGCGTTGGTCAACACTAGCGTTGTGTTTAATCCGAGGAATCCTTCTGTGAAACCTGTTGATGCGTTTAGGGCTTTGTTCGGCGATGATTACTACATTTGCAGGTTTCAGGAGCCTGGAGAGATCGAAGAGGACTTTGCTCAAGTTGATACAAAAAAGTTAATAACCAGATTTTTCACTTCGCGTAACCCACGTCCACCTTGCATTCCAAAGTCAGTTGGTTTCAGAGGTTTACCTGATCCACCTTCTTTGCCCGCTTGGCTCACCGAACAAGATGTCAGGTTCTACGGAGACAAGTTTAGCCAAAAAGGCTTCACCGGTGGGCTAAACTACTATCGCGCCCTGAACCTAAGCTGGGAACTAACAGCTCCATGGACCGGTCTACAAATCAAAGTCCCTGTGAAATTCATAGTGGGTGATCTAGACATAACATACAATATCCCCGGAACAAAGGAATACATACATGAAGGTGGTTTGAAGAAGCACGTACCTTTTCTACAAGAAGTGGTGGTAATGGAAGGTGTAGGTCACTTCCTCCATCAAGAGAAGCCTGACGAGGTCACTGACCATATCTATGGCTTCTTCAAGAAATTCAGAACCCGCGAAACCGCATCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1 Abhydrolase_6

Protein Analysis

324

Amino Acids

36.66

Weight (kDa)

6.26

Isoelectric Point (pI)

32.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 25 - 300 2.8e-26 alpha/beta hydrolase fold
Hydrolase_4 PF12146 26 - 139 1.9e-08 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 27 - 306 3.9e-22 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02340
fragaria_vesca FvH4_2g25930
malus_domestica MD03G1290700.v1.1 MD11G1307800.v1.1
prunus_persica Prupe.8G263700_v2.0.a1
pyrus_communis pycom11g26910
rosa_chinensis RchiOBHm_Chr6g0294251
rosa_laevigata RLG00000011885
rosa_multiflora Rmu_sc0002462.1_g000006
rosa_roxburghii Rroxscaffold_7G00173510
rosa_rugosa Rorug06G0242900
rosa_samantha Rh6AG355300 Rh6CG369400 Rh6DG356100
rosa_wichuraiana Rw6G030950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 447
AccI GTMKAC 1 cut(s) 736
AccII CGCG 5 cut(s) 159, 359, 538, 693, 957
AciI CCGC 5 cut(s) 157, 203, 609, 955, 963
AclWI GGATC 2 cut(s) 73, 587
AcsI RAATTY 2 cut(s) 758, 944
AcyI GRCGYC 1 cut(s) 121
AfaI GTAC 2 cut(s) 113, 843
AfiI CCNNNNNNNGG 3 cut(s) 108, 709, 731
AflIII ACRYGT 1 cut(s) 357
AgeI ACCGGT 2 cut(s) 671, 731
AgsI TTSAA 2 cut(s) 832, 940
AjiI CACGTC 1 cut(s) 548
AjnI CCWGG 1 cut(s) 469
AluBI AGCT 3 cut(s) 130, 708, 722
AluI AGCT 3 cut(s) 130, 708, 722
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 4 cut(s) 139, 213, 237, 639
AlwI GGATC 2 cut(s) 73, 587
AoxI GGCC 1 cut(s) 341
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 2 cut(s) 758, 944
ArsI GACNNNNNNTTYG 2 cut(s) 615, 647
AsiGI ACCGGT 2 cut(s) 671, 731
AspLEI GCGC 3 cut(s) 161, 202, 695
AspS9I GGNCC 2 cut(s) 71, 729
AsuC2I CCSGG 1 cut(s) 798
AsuHPI GGTGA 4 cut(s) 116, 610, 661, 782
AvaII GGWCC 2 cut(s) 71, 729
AxyI CCTNAGG 1 cut(s) 148
BauI CACGAG 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 1 cut(s) 130
BccI CCATC 2 cut(s) 233, 900
BceAI ACGGC 2 cut(s) 190, 202
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 1 cut(s) 471
BciVI GTATCC 1 cut(s) 176
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 798
BcoDI GTCTC 4 cut(s) 139, 213, 237, 639
BfaI CTAG 2 cut(s) 372, 776
BfuAI ACCTGC 1 cut(s) 447
BfuI GTATCC 1 cut(s) 176
BglI GCCNNNNNGGC 2 cut(s) 613, 663
BglII AGATCT 1 cut(s) 172
BisI GCNGC 2 cut(s) 144, 203
BlsI GCNGC 2 cut(s) 145, 204
Bme1390I CCNGG 2 cut(s) 471, 798
Bme18I GGWCC 2 cut(s) 71, 729
BmgBI CACGTC 1 cut(s) 548
BmgT120I GGNCC 2 cut(s) 71, 729
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 2 cut(s) 471, 798
BmsI GCATC 2 cut(s) 187, 406
BpmI CTGGAG 2 cut(s) 153, 492
Bpu10I CCTNAGC 1 cut(s) 704
BpuEI CTTGAG 1 cut(s) 480
BpuMI CCSGG 1 cut(s) 798
BsaAI YACGTR 1 cut(s) 841
BsaHI GRCGYC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 237
BsaJI CCNNGG 4 cut(s) 91, 389, 725, 796
BsaWI WCCGGW 2 cut(s) 671, 731
BsaXI ACNNNNNCTCC 4 cut(s) 234, 264, 300, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 709, 731
Bse118I RCCGGY 2 cut(s) 671, 731
Bse1I ACTGG 2 cut(s) 74, 264
Bse21I CCTNAGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 471
BseDI CCNNGG 4 cut(s) 91, 389, 725, 796
BseGI GGATG 2 cut(s) 225, 965
BseLI CCNNNNNNNGG 3 cut(s) 108, 709, 731
BseNI ACTGG 2 cut(s) 74, 264
BseXI GCAGC 1 cut(s) 130
BseYI CCCAGC 1 cut(s) 708
Bsh1236I CGCG 5 cut(s) 159, 359, 538, 693, 957
BshFI GGCC 1 cut(s) 343
BshTI ACCGGT 2 cut(s) 671, 731
BsiHKAI GWGCWC 1 cut(s) 18
BsiSI CCGG 3 cut(s) 672, 732, 798
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 3 cut(s) 108, 709, 731
BsmAI GTCTC 4 cut(s) 139, 213, 237, 639
BsmBI CGTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 734
BsmI GAATGC 1 cut(s) 558
BsnI GGCC 1 cut(s) 343
Bso31I GGTCTC 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 18
Bsp143I GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
Bsp19I CCATGG 2 cut(s) 91, 725
BspACI CCGC 5 cut(s) 157, 203, 609, 955, 963
BspANI GGCC 1 cut(s) 343
BspFNI CGCG 5 cut(s) 159, 359, 538, 693, 957
BspLI GGNNCC 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 447
BspPI GGATC 2 cut(s) 73, 587
BspTNI GGTCTC 1 cut(s) 237
BsrFI RCCGGY 2 cut(s) 671, 731
BsrI ACTGG 2 cut(s) 74, 264
BssAI RCCGGY 2 cut(s) 671, 731
BssECI CCNNGG 4 cut(s) 91, 389, 725, 796
BssMI GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
BssNI GRCGYC 1 cut(s) 121
BssSI CACGAG 1 cut(s) 210
BssT1I CCWWGG 2 cut(s) 91, 725
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 841
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 2 cut(s) 148, 704
BstDSI CCRYGG 2 cut(s) 91, 725
BstEII GGTNACC 1 cut(s) 152
BstF5I GGATG 2 cut(s) 225, 965
BstFNI CGCG 5 cut(s) 159, 359, 538, 693, 957
BstHHI GCGC 3 cut(s) 161, 202, 695
BstKTI GATC 9 cut(s) 81, 106, 175, 280, 339, 349, 480, 595, 775
BstMAI GTCTC 4 cut(s) 139, 213, 237, 639
BstMBI GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
BstMWI GCNNNNNNNGC 5 cut(s) 127, 317, 425, 613, 663
BstNI CCWGG 1 cut(s) 471
BstNSI RCATGY 2 cut(s) 49, 53
BstPI GGTNACC 1 cut(s) 152
BstSCI CCNGG 2 cut(s) 469, 796
BstUI CGCG 5 cut(s) 159, 359, 538, 693, 957
BstV1I GCAGC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 172
BstXI CCANNNNNNTGG 1 cut(s) 929
BstYI RGATCY 1 cut(s) 172
Bsu36I CCTNAGG 1 cut(s) 148
BsuI GTATCC 1 cut(s) 176
BsuRI GGCC 1 cut(s) 343
BtgI CCRYGG 2 cut(s) 91, 725
BtgZI GCGATG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 548
BtsCI GGATG 2 cut(s) 225, 965
BtsIMutI CAGTG 2 cut(s) 81, 915
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 1 cut(s) 609
CfoI GCGC 3 cut(s) 161, 202, 695
Cfr10I RCCGGY 2 cut(s) 671, 731
Cfr13I GGNCC 2 cut(s) 71, 729
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 112, 842
CspAI ACCGGT 2 cut(s) 671, 731
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviAII CATG 6 cut(s) 46, 50, 92, 117, 726, 818
CviQI GTAC 2 cut(s) 112, 842
DdeI CTNAG 2 cut(s) 148, 704
DpnI GATC 9 cut(s) 80, 105, 174, 279, 338, 348, 479, 594, 774
DpnII GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 91, 725
Eco147I AGGCCT 1 cut(s) 343
Eco31I GGTCTC 1 cut(s) 237
Eco32I GATATC 1 cut(s) 27
Eco47I GGWCC 2 cut(s) 71, 729
Eco81I CCTNAGG 1 cut(s) 148
Eco91I GGTNACC 1 cut(s) 152
EcoO65I GGTNACC 1 cut(s) 152
EcoRII CCWGG 1 cut(s) 469
EcoRV GATATC 1 cut(s) 27
EcoT14I CCWWGG 2 cut(s) 91, 725
EcoT22I ATGCAT 1 cut(s) 51
ErhI CCWWGG 2 cut(s) 91, 725
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 6 cut(s) 49, 53, 95, 120, 729, 821
FaqI GGGAC 1 cut(s) 734
FatI CATG 6 cut(s) 45, 49, 91, 116, 725, 817
FauI CCCGC 2 cut(s) 616, 962
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 1 cut(s) 736
Fnu4HI GCNGC 2 cut(s) 144, 203
FokI GGATG 2 cut(s) 212, 952
Fsp4HI GCNGC 2 cut(s) 144, 203
FspBI CTAG 2 cut(s) 372, 776
GlaI GCGC 3 cut(s) 160, 201, 694
GluI GCNGC 2 cut(s) 144, 203
GsaI CCCAGC 1 cut(s) 712
GsuI CTGGAG 2 cut(s) 153, 492
HaeIII GGCC 1 cut(s) 343
HapII CCGG 3 cut(s) 672, 732, 798
HgaI GACGC 1 cut(s) 110
HhaI GCGC 3 cut(s) 161, 202, 695
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 6 cut(s) 49, 53, 95, 120, 729, 821
Hin6I GCGC 3 cut(s) 159, 200, 693
HinP1I GCGC 3 cut(s) 159, 200, 693
HincII GTYRAC 2 cut(s) 355, 367
HindII GTYRAC 2 cut(s) 355, 367
HinfI GANTC 5 cut(s) 191, 215, 263, 351, 394
HpaII CCGG 3 cut(s) 672, 732, 798
HphI GGTGA 4 cut(s) 116, 610, 661, 782
Hpy166II GTNNAC 5 cut(s) 355, 367, 551, 587, 737
Hpy188I TCNGA 6 cut(s) 196, 242, 254, 390, 581, 950
Hpy188III TCNNGA 8 cut(s) 101, 170, 212, 299, 464, 776, 896, 940
Hpy8I GTNNAC 5 cut(s) 355, 367, 551, 587, 737
Hpy99I CGWCG 4 cut(s) 25, 242, 254, 296
HpyAV CCTTC 5 cut(s) 239, 408, 609, 815, 866
HpyCH4IV ACGT 2 cut(s) 547, 840
HpyCH4V TGCA 3 cut(s) 49, 456, 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 317, 425, 613, 663
HpyF3I CTNAG 2 cut(s) 148, 704
HpySE526I ACGT 2 cut(s) 547, 840
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 6 cut(s) 49, 53, 95, 120, 729, 821
HspAI GCGC 3 cut(s) 159, 200, 693
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
LmnI GCTCC 4 cut(s) 172, 308, 466, 727
Lsp1109I GCAGC 1 cut(s) 130
LweI GCATC 2 cut(s) 187, 406
MaeI CTAG 2 cut(s) 372, 776
MaeII ACGT 2 cut(s) 547, 840
MaeIII GTNAC 6 cut(s) 122, 152, 296, 539, 881, 913
MalI GATC 9 cut(s) 80, 105, 174, 279, 338, 348, 479, 594, 774
MboI GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
MboII GAAGA 5 cut(s) 19, 167, 494, 844, 928
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 18
MluCI AATT 3 cut(s) 312, 758, 944
MluI ACGCGT 1 cut(s) 357
MlyI GAGTC 1 cut(s) 360
MmeI TCCRAC 2 cut(s) 220, 232
MnlI CCTC 8 cut(s) 173, 257, 299, 384, 478, 575, 899, 904
Mph1103I ATGCAT 1 cut(s) 51
MseI TTAA 3 cut(s) 42, 384, 515
MslI CAYNNNNRTG 3 cut(s) 50, 822, 927
MspI CCGG 3 cut(s) 672, 732, 798
MspR9I CCNGG 2 cut(s) 471, 798
MunI CAATTG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 558
MvaI CCWGG 1 cut(s) 471
MvnI CGCG 5 cut(s) 159, 359, 538, 693, 957
MwoI GCNNNNNNNGC 5 cut(s) 127, 317, 425, 613, 663
NciI CCSGG 1 cut(s) 798
NcoI CCATGG 2 cut(s) 91, 725
NdeII GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
NlaIII CATG 6 cut(s) 49, 53, 95, 120, 729, 821
NlaIV GGNNCC 1 cut(s) 468
NmuCI GTSAC 4 cut(s) 122, 296, 881, 913
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 49, 53
PceI AGGCCT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 246, 297
PctI GAATGC 1 cut(s) 558
PfeI GAWTC 4 cut(s) 191, 215, 263, 394
PflFI GACNNNGTC 2 cut(s) 248, 911
PinAI ACCGGT 2 cut(s) 671, 731
PkrI GCNGC 2 cut(s) 145, 204
PleI GAGTC 1 cut(s) 359
PpsI GAGTC 1 cut(s) 359
Ppu21I YACGTR 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 469
PspEI GGTNACC 1 cut(s) 152
PspFI CCCAGC 1 cut(s) 708
PspGI CCWGG 1 cut(s) 469
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 2 cut(s) 71, 729
PsuI RGATCY 1 cut(s) 172
PsyI GACNNNGTC 2 cut(s) 248, 911
RsaI GTAC 2 cut(s) 113, 843
RsaNI GTAC 2 cut(s) 112, 842
RseI CAYNNNNRTG 3 cut(s) 50, 822, 927
SaqAI TTAA 3 cut(s) 42, 384, 515
SatI GCNGC 2 cut(s) 144, 203
Sau3AI GATC 9 cut(s) 78, 103, 172, 277, 336, 346, 477, 592, 772
Sau96I GGNCC 2 cut(s) 71, 729
SchI GAGTC 1 cut(s) 360
ScrFI CCNGG 2 cut(s) 471, 798
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 2 cut(s) 187, 406
SgrAI CRCCGGYG 1 cut(s) 671
SinI GGWCC 2 cut(s) 71, 729
SmiMI CAYNNNNRTG 3 cut(s) 50, 822, 927
SmlI CTYRAG 1 cut(s) 495
SmoI CTYRAG 1 cut(s) 495
Sse9I AATT 3 cut(s) 312, 758, 944
SseBI AGGCCT 1 cut(s) 343
SsiI CCGC 5 cut(s) 157, 203, 609, 955, 963
SspMI CTAG 2 cut(s) 372, 776
StuI AGGCCT 1 cut(s) 343
StyD4I CCNGG 2 cut(s) 469, 796
StyI CCWWGG 2 cut(s) 91, 725
TaiI ACGT 2 cut(s) 550, 843
TaqI TCGA 4 cut(s) 59, 178, 349, 480
TaqII GACCGA 1 cut(s) 284
TasI AATT 3 cut(s) 312, 758, 944
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 4 cut(s) 191, 215, 263, 394
Tru1I TTAA 3 cut(s) 42, 384, 515
Tru9I TTAA 3 cut(s) 42, 384, 515
TscAI CASTG 2 cut(s) 81, 922
TseFI GTSAC 4 cut(s) 122, 296, 881, 913
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 4 cut(s) 122, 296, 881, 913
TspDTI ATGAA 3 cut(s) 221, 751, 834
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 2 cut(s) 81, 922
Tth111I GACNNNGTC 2 cut(s) 248, 911
VpaK11BI GGWCC 2 cut(s) 71, 729
XapI RAATTY 2 cut(s) 758, 944
XbaI TCTAGA 1 cut(s) 775
XceI RCATGY 2 cut(s) 49, 53
XmiI GTMKAC 1 cut(s) 736
XspI CTAG 2 cut(s) 372, 776
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.