AT4G12020

histone deacetylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0017954
Physical Location & Seq
Forward (+)
7201522 .. 7209692
8171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12020.1

Sequence Viewer

Length: 5397 bp
ATGTCGGAGAAGGAAGAACTTCCGTTGACATTGACGTCCATCGGAGCGGCCACCGCGACTAGTGATTATCATCAGAGAGTAGGAAGTTCCGGTGAAGGGATTAGTAGCTCGAGTAGTGATGTTGACCCGAGGTTCATGCAGAATAGCCCCACGGGTTTGATGATTTCCCAATCGTCGTCGATGTGCACCGTACCGCCTGGCATGGCAGCAACACCACCAATAAGCTCAGGTTCCGGTTTATCTCAGCAGCTTAATAATTCTTCTAGTTCCAAGTTATGTCAAGTGGAAGGATGTCAAAAAGGAGCAAGAGATGCATCTGGTCGTTGCATTTCCCATGGCGGTGGACGTAGATGCCAGAAACCTGATTGCCAGAAGGGAGCTGAAGGTAAAACAGTGTACTGTAAAGCCCACGGAGGTGGTCGCAGATGTGAATATCTTGGATGCACCAAAGGCGCAGAAGGCAGTACTGATTTTTGTATAGCTCATGGAGGTGGTCGAAGATGCAACCATGAAGATTGCACACGATCTGCTTGGGGAAGAACAGAATTCTGTGTCAAGCACGGTGGAGGAGCGAGATGCAAAACATACGGCTGCGGAAAAAGCGCTAGTGGTCCTTTGCCATTCTGCCGAGCCCATGGTGGTGGTAAAAAATGCAGCCATGAAGATTGCACAGGATTTGCTAGGGGAAGATCAGGACTCTGTCTCATGCACGGTGGGGGAAAGAGATGCCAAAGAGAGAACTGCACTAAAAGCGCTGAAGGTCTTTCGGGACTCTGCATATCCCATGGTGGTGGTCGGCGATGTCAATCTATTGGATGCACAAAAGGAGCGAAAGGGAGCAAAATGTTCTGCAAAGCATGCATAACTAAAAGGCCTCTAACGATTGATGGAGGAGGAAATATGGGAGGGGTAACAACAGGTGATGCCTTGAACTATCTCAAAGCTGTGAAGGACAAGTTTGAAGACAGTGAGAAATATGACACTTTCCTTGAAGTCTTGAATGACTGTAAACATCAGGGAGTTGACACTAGTGGCGTCATAGCCAGATTAAAAGATTTGTTCAAGGGCCATGACGACTTACTTTTGGGTTTTAATACCTACTTGTCAAAGGAGTACCAAATAACCATTCTGCCCGAGGATGATTTCCCTATCGATTTTCTTGACAAGGTTGAGGGACCTTATGAAATGACATATCAGCAAGCTCAAACAGTTCAAGCCAATGCCAATATGCAACCTCAAACTGAGTACCCTTCTTCCTCTGCGGTTCAATCATTTTCATCGGGTCAACCTCAGATCCCCACCTCAGCTCCGGATTCTTCACTACTAGCTAAAAGTAATACCTCAGGTATAACTATCATCGAGCACATGTCACAACAGCCTCTAAATGTTGACAAACAAGTTAATGATGGCTATAACTGGCAAAAGTATGGGCAAAAGAAAGTTAAAGGCAGCAAGTTTCCTCTAAGCTATTACAAGTGCACATATCTAGGATGTCCTTCCAAGAGGAAGGTTGAGAGATCTCTTGATGGACAAGTAGCAGAAATCGTCTACAAAGATCGACACAATCACGAACCTCCTAACCAAGGAAAAGATGGTAGCACCACATATCTAAGTGGGAGTTCGACACACATCAATTGCATGAGCTCTGAATTGACAGCATCACAGTTTAGCTCCAACAAGACTAAGATAGAGCAACAGGAAGCAGCAAGTCTAGCTACGACAATAGAGTACATGTCTGAGGCAAGTGACAATGAAGAAGACAGTAATGGAGAAACTAGTGAGGGAGAGAAAGATGAAGACGAGCCTGAACCAAAGAGAAGAATTACAGAAGTTCAGGTTTCGGAACTAGCTGATGCTTCAGATAGAACCGTGAGAGAGCCTAGGGTTATTTTCCAAACAACGAGTGAAGTTGATAATTTAGATGATGGATATAGGTGGCGGAAATATGGACAGAAAGTTGTTAAAGGGAATCCTTATCCAAGGTTTTCCTCCTCTAAAGATTATGATGTCGTAATCAGATACGGAAGAGCAGATATAAGCAATGAGGATTTCATTAGCCATCTTCGTGCTTCCCTCTGCCGGAGAGGGATTTCTGTCTATGAAAAATTTAATGAAGTGGATGCACTTCCAAAATGTAGGGTTTTGATTATAGTATTAACAAGCACATATGTCCCTTCGAACCTCTTAAACATTCTTGAACACCAACATACAGAGGATCGAGTGGTTTATCCAATTTTCTACAGACTATCACCATATGATTTTGTCTGTAACAGCAAGAATTATGAGAGATTTTATCTCCAAGATGAGCCAAAAAAATGGCAAGCTGCTTTGAAGGAAATAACTCAGATGCCTGGCTACACATTGACAGATAAGTCTGAATCTGAACTTATAGATGAGATTGTAAGAGATGCTTTAAAGGTGCTATGTTCTGCTGATAAGGTGAACATGATTGGGATGGATATGCAAGTAGAGGAGATTTTGTCACTGCTATGCATTGAGTCCCTTGATGTTCGCAGCATTGGTATATGGGGTACAGTTGGTATAGGAAAAACAACCATTGCTGAAGAGATCTTTCGCAAAATCTCTGTCCAATATGAGACCTGTGTCGTCCTTAAGGACCTCCACAAAGAAGTTGAGGTAAAAGGTCACGATGCTGTGAGAGAGAATTTTCTGTCTGAAGTTTTAGAGGTAGAACCTCATGTTATCCGGATATCTGACATTAAAACAAGCTTCTTGAGAAGTCGGCTTCAGCGTAAAAGGATCCTTGTTATTCTTGACGATGTGAATGATTACAGAGATGTTGACACCTTTTTGGGGACGCTTAACTATTTTGGTCCAGGAAGCAGAATAATCATGACCTCTAGAAATAGACGTGTTTTCGTACTATGTAAAATCGATCATGTCTATGAGGTTAAGCCATTAGATATTCCTAAGTCTCTACTACTTCTTGATCGTGGGACATGTCAAATTGTTTTGTCACCTGAGGTTTACAAGACATTGTCACTTGAGCTGGTCAAATTTTCAAATGGAAATCCCCAGGTTCTTCAGTTCTTGAGCAGTATTGACAGAGAATGGAATAAGTTATCACAAGAAGTTAAGACAACATCTCCCATTTACATCCCAGGTATATTTGAAAAGAGCTGTTGTGGGCTTGATGACAACGAGAGGGGTATATTTTTGGACATTGCATGTTTCTTTAATAGGATTGATAAAGACAATGTCGCAATGTTGCTGGATGGTTGTGGTTTCTCTGCACATGTCGGATTTAGAGGCCTTGTTGACAAATCACTGTTGACAATATCACAACACAACTTGGTGGACATGCTCAGTTTTATCCAGGCAACTGGTCGAGAAATTGTTCGCCAAGAATCAGCTGACAGACCAGGAGACCGCAGCAGGTTGTGGAATGCCGACTATATCAGACACGTATTCATAAATGACACTGGCACATCAGCTATTGAGGGCATTTTCCTAGACATGTTGAATCTTAAATTTGATGCAAATCCCAACGTGTTCGAGAAAATGTGTAACCTTAGACTGTTGAAATTGTATTGCTCCAAAGCGGAAGAGAAGCATGGAGTATCTTTTCCACAAGGTCTTGAATATTTGCCGAGCAAGCTAAGGCTTCTCCATTGGGAATATTATCCTCTAAGTTCTTTGCCGAAAAGTTTTAATCCAGAGAACCTTGTCGAGCTTAACTTGCCAAGTAGCTGTGCAAAGAAACTTTGGAAAGGAAAAAAGGCAAGGTTTTGTACAACCAATTCAAGTCTGGAAAAGCTTAAAAAGATGAGACTTAGCTACTCCGACCAGTTAACTAAAATCCCAAGACTTTCAAGCGCAACAAATCTTGAGCATATTGATCTTGAAGGTTGCAACAGTTTGTTGAGCCTTAGCCAGTCCATTTCTTATCTTAAGAAGCTTGTTTTTCTGAATTTAAAGGGCTGCTCGAAGCTGGAGAATATTCCATCTATGGTTGATTTAGAATCGCTTGAGGTTCTAAATCTTTCGGGTTGTTCAAAGCTAGGGAACTTCCCGGAGATCTCACCAAATGTGAAAGAACTGTACATGGGTGGGACTATGATACAAGAAATCCCGTCATCGATTAAGAACTTGGTATTGCTTGAGAAACTGGACCTGGAAAACAGTAGACATCTCAAGAATCTTCCAACAAGCATCTACAAGTTGAAGCATCTTGAAACTCTAAATCTTTCAGGCTGCATAAGCCTGGAGCGATTTCCAGACTCGTCGAGAAGGATGAAATGCTTAAGGTTTTTGGATTTAAGCAGGACAGACATTAAAGAGCTGCCCTCTTCCATATCGTATCTGACTGCTCTTGACGAACTATTATTCGTAGACTCCAGGAGAAACTCGCCAGTTGTAACCAATCCCAATGCCAATTCAACTGAGTTGATGCCTTCTGAGTCAAGTAAGCTTGAGATCTTAGGTACTCCGGCAGATAACGAAGTAGTTGTTGGTGGTACGGTAGAGAAAACCCGTGGTATTGAACGAACGCCGACTATTTTGGTGAAGTCGAGAGAGTATCTAATTCCCGATGATGTTGTGGCGGTTGGTGGTGATATTAAGGGGCTAAGACCACCAGTACTTCAGCTCCAACCAGCAATGAAACTATCTCATATTCCTCGAGGATCAACTTGGGATTTCGTTACGCATTTCGCTCCACCTGAAACAGTTGCGCCGCCGAGTTCCTCTTCAGAAGCCAGGGAAGAGGAAGTGGAAACGGAAGAGACGGGAGCTATGTTTATCCCATTGGGGGATAAGGAGACATGCTCATTCACTGTAAACAAGGGTGACTCCTCAAGGACAATATCTAATACGTCGCCGATTTATGCCTCCGAAGGATCTTTCATCACGTGTTGGCAGAAGGGTCAACTTCTGGGACGAGGATCATTAGGGTCCGTATATGAAGGCATTTCAGCAGACGGGGACTTCTTTGCTTTCAAGGAAGTTTCACTACTTGATCAGGGAAGTCAGGCACATGAATGGATACAACAAGTCGAGGGGGGGATTGCGCTACTTAGTCAGCTTCAGCATCAGAATATCGTGCGATATCGTGGCACAACTAAGGACGAGTCGAATTTGTACATTTTTCTTGAACTTGTAACCCAAGGGTCCCTTCGAAAACTCTACCAAAGAAACCAGCTTGGGGACTCTGTAGTCTCCTTATACACAAGACAGATTCTTGATGGATTGAAATATCTCCACGATAAAGGTTTTATACACAGGAACATTAAATGTGCAAATGTATTGGTGGACGCTAATGGAACAGTTAAACTTGCAGATTTTGGATTGGCTAAGGTAATGTCCCTCTGGCGAACTCCGTATTGGAATTGGATGGCTCCAGAGGTTATTGTTTTAAAATCCTTCCCGCTGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0001101 GO:0001708 GO:0001932 GO:0001934 GO:0002119 GO:0002164 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006355 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007423 GO:0007548 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009507 GO:0009526 GO:0009536 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009653 GO:0009791 GO:0009887 GO:0009888 GO:0009889 GO:0009893 GO:0009941 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010468 GO:0010556 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022414 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030154 GO:0030539 GO:0031098 GO:0031323 GO:0031325 GO:0031326 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031967 GO:0031975 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035112 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0040011 GO:0040025 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045165 GO:0045859 GO:0045860 GO:0045937 GO:0046661 GO:0046777 GO:0048364 GO:0048507 GO:0048513 GO:0048518 GO:0048522 GO:0048569 GO:0048583 GO:0048584 GO:0048589 GO:0048608 GO:0048731 GO:0048806 GO:0048808 GO:0048856 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051252 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0061458 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0090596 GO:0090597 GO:0090598 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:0140110 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko04016 ko04139 ko04626 ko04919 ko05016 ko05202 map04016 map04139 map04626 map04919 map05016 map05202
Pfam Domains
Protein Families
PAH Pkinase

Protein Analysis

1798

Amino Acids

199.99

Weight (kDa)

6.64

Isoelectric Point (pI)

41.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zf_WRKY19 PF24906 91 - 114 6.9e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 115 - 139 2.1e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 140 - 164 1.2e-07 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 165 - 189 1.1e-06 WRKY19 zinc finger domain
Zf_WRKY19 PF24906 240 - 264 1e-09 WRKY19 zinc finger domain
PAH PF02671 324 - 368 3.8e-15 Paired amphipathic helix repeat
WRKY PF03106 468 - 524 4.9e-22 WRKY DNA -binding domain
WRKY PF03106 641 - 661 2.1e-06 WRKY DNA -binding domain
NB-ARC PF00931 822 - 984 3e-15 NB-ARC domain
WHD_ROQ1 PF23282 1053 - 1124 2.3e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 1320 - 1422 1.9e-09 Leucine-rich repeat region
LRR_13 PF23286 1327 - 1396 1.6e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_8 PF13855 1374 - 1432 7.9e-06 Leucine rich repeat
Pkinase PF00069 1626 - 1790 2.2e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1629 - 1793 1.1e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 34, 2371, 5174
AatII GACGTC 1 cut(s) 38
AbsI CCTCGAGG 1 cut(s) 4641
Acc36I ACCTGC 1 cut(s) 3387
AccBSI CCGCTC 1 cut(s) 47
AccI GTMKAC 3 cut(s) 1548, 4147, 4353
AccII CGCG 1 cut(s) 56
AccIII TCCGGA 2 cut(s) 1309, 2706
AclWI GGATC 7 cut(s) 1288, 2223, 2755, 2768, 4654, 4867, 4912
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 7 cut(s) 545, 2105, 2665, 3017, 3491, 3931, 5095
AcvI CACGTG 1 cut(s) 4872
AcyI GRCGYC 2 cut(s) 35, 1035
AfeI AGCGCT 2 cut(s) 604, 754
AfiI CCNNNNNNNGG 6 cut(s) 96, 1583, 2079, 2814, 4771, 5164
AflII CTTAAG 3 cut(s) 2612, 3911, 4264
AflIII ACRYGT 9 cut(s) 1365, 1731, 2872, 2960, 3256, 3424, 3477, 3510, 4871
AhlI ACTAGT 3 cut(s) 59, 1028, 1775
AjiI CACGTC 1 cut(s) 2873
AjuI GAANNNNNNNTTGG 8 cut(s) 1110, 1142, 4455, 4487, 5118, 5150, 5326, 5358
AleI CACNNNNGTG 1 cut(s) 414
Alw21I GWGCWC 4 cut(s) 188, 1365, 1481, 1646
Alw26I GTCTC 8 cut(s) 707, 2591, 2940, 3381, 3784, 4739, 4775, 5182
Alw44I GTGCAC 2 cut(s) 184, 1477
AlwI GGATC 7 cut(s) 1288, 2223, 2755, 2768, 4654, 4867, 4912
Ama87I CYCGRG 4 cut(s) 109, 127, 1133, 4641
Aor13HI TCCGGA 2 cut(s) 1309, 2706
Aor51HI AGCGCT 2 cut(s) 604, 754
AoxI GGCC 4 cut(s) 48, 872, 1066, 3271
ApaLI GTGCAC 2 cut(s) 184, 1477
ApoI RAATTY 7 cut(s) 545, 2105, 2665, 3017, 3491, 3931, 5095
ArsI GACNNNNNNTTYG 2 cut(s) 2571, 2603
Asp700I GAANNNNTTC 2 cut(s) 18, 3357
AspA2I CCTAGG 1 cut(s) 1880
AspLEI GCGC 6 cut(s) 455, 605, 755, 3839, 4696, 5032
AspS9I GGNCC 8 cut(s) 611, 1066, 1175, 2617, 2834, 4132, 4914, 5130
AsuC2I CCSGG 1 cut(s) 4034
AsuHPI GGTGA 9 cut(s) 104, 932, 2241, 2452, 2970, 4035, 4537, 4586, 4820
AsuII TTCGAA 2 cut(s) 2177, 5137
AvaI CYCGRG 4 cut(s) 109, 127, 1133, 4641
AvaII GGWCC 7 cut(s) 611, 1175, 2617, 2834, 4132, 4914, 5130
AvrII CCTAGG 1 cut(s) 1880
AxyI CCTNAGG 2 cut(s) 1342, 2982
BaeGI GKGCMC 2 cut(s) 188, 1481
BamHI GGATCC 1 cut(s) 2760
BanII GRGCYC 2 cut(s) 634, 1646
BarI GAAGNNNNNNTAC 2 cut(s) 4956, 4988
BbrPI CACGTG 1 cut(s) 4872
BbsI GAAGAC 3 cut(s) 969, 1764, 1803
Bbv12I GWGCWC 4 cut(s) 188, 1365, 1481, 1646
BbvCI CCTCAGC 1 cut(s) 1303
BceAI ACGGC 1 cut(s) 604
BcgI CGANNNNNNTGC 2 cut(s) 118, 152
BciVI GTATCC 1 cut(s) 4998
BclI TGATCA 1 cut(s) 4978
BcnI CCSGG 1 cut(s) 4034
BcoDI GTCTC 8 cut(s) 707, 2591, 2940, 3381, 3784, 4739, 4775, 5182
BcuI ACTAGT 3 cut(s) 59, 1028, 1775
BfmI CTRYAG 2 cut(s) 2239, 5172
BfoI RGCGCY 2 cut(s) 606, 756
BfrI CTTAAG 3 cut(s) 2612, 3911, 4264
BfuAI ACCTGC 1 cut(s) 3387
BfuI GTATCC 1 cut(s) 4998
BglII AGATCT 4 cut(s) 1517, 2568, 4038, 4437
BlnI CCTAGG 1 cut(s) 1880
BmcAI AGTACT 2 cut(s) 466, 4602
Bme18I GGWCC 7 cut(s) 611, 1175, 2617, 2834, 4132, 4914, 5130
BmeT110I CYCGRG 4 cut(s) 109, 127, 1133, 4641
BmgBI CACGTC 1 cut(s) 2873
BmgT120I GGNCC 8 cut(s) 611, 1066, 1175, 2617, 2834, 4132, 4914, 5130
BmiI GGNNCC 7 cut(s) 232, 1176, 2762, 4915, 5131, 5132, 5358
BoxI GACNNNNGTC 1 cut(s) 2603
BpiI GAAGAC 3 cut(s) 969, 1764, 1803
BplI GAGNNNNNCTC 4 cut(s) 4292, 4324, 4772, 4804
BpmI CTGGAG 4 cut(s) 3974, 4247, 4342, 5343
Bpu10I CCTNAGC 5 cut(s) 226, 1303, 3620, 3890, 5313
Bpu14I TTCGAA 2 cut(s) 2177, 5137
BpuEI CTTGAG 9 cut(s) 2755, 3026, 3073, 3869, 4010, 4139, 4142, 4454, 4801
BpuMI CCSGG 1 cut(s) 4034
Bsa29I ATCGAT 3 cut(s) 1152, 2895, 4100
BsaAI YACGTR 2 cut(s) 3427, 4872
BsaBI GATNNNNATC 3 cut(s) 69, 805, 3501
BsaHI GRCGYC 2 cut(s) 35, 1035
BsaI GGTCTC 2 cut(s) 2591, 3381
BsaWI WCCGGW 4 cut(s) 89, 233, 1309, 2706
BsaXI ACNNNNNCTCC 8 cut(s) 1291, 1321, 2465, 2495, 3091, 3121, 4593, 4623
Bsc4I CCNNNNNNNGG 6 cut(s) 96, 1583, 2079, 2814, 4771, 5164
Bse1I ACTGG 8 cut(s) 1421, 3349, 3448, 3808, 3895, 4134, 4373, 4598
Bse21I CCTNAGG 2 cut(s) 1342, 2982
Bse3DI GCAATG 5 cut(s) 2047, 2556, 3183, 3231, 4626
Bse8I GATNNNNATC 3 cut(s) 69, 805, 3501
BseAI TCCGGA 2 cut(s) 1309, 2706
BseCI ATCGAT 3 cut(s) 1152, 2895, 4100
BseJI GATNNNNATC 3 cut(s) 69, 805, 3501
BseLI CCNNNNNNNGG 6 cut(s) 96, 1583, 2079, 2814, 4771, 5164
BseMI GCAATG 5 cut(s) 2047, 2556, 3183, 3231, 4626
BseNI ACTGG 8 cut(s) 1421, 3349, 3448, 3808, 3895, 4134, 4373, 4598
BseRI GAGGAG 5 cut(s) 582, 906, 1981, 2486, 4804
BseSI GKGCMC 2 cut(s) 188, 1481
BsgI GTGCAG 2 cut(s) 727, 3237
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 4 cut(s) 50, 874, 1068, 3273
BshVI ATCGAT 3 cut(s) 1152, 2895, 4100
BsiHKAI GWGCWC 4 cut(s) 188, 1365, 1481, 1646
BsiHKCI CYCGRG 4 cut(s) 109, 127, 1133, 4641
BsiSI CCGG 7 cut(s) 90, 234, 1310, 2080, 2707, 4034, 4451
BslI CCNNNNNNNGG 6 cut(s) 96, 1583, 2079, 2814, 4771, 5164
BsmAI GTCTC 8 cut(s) 707, 2591, 2940, 3381, 3784, 4739, 4775, 5182
BsmBI CGTCTC 1 cut(s) 4739
BsmI GAATGC 1 cut(s) 3412
BsnI GGCC 4 cut(s) 50, 874, 1068, 3273
Bso31I GGTCTC 2 cut(s) 2591, 3381
BsoBI CYCGRG 4 cut(s) 109, 127, 1133, 4641
Bsp119I TTCGAA 2 cut(s) 2177, 5137
Bsp1286I GDGCHC 5 cut(s) 188, 634, 1365, 1481, 1646
Bsp13I TCCGGA 2 cut(s) 1309, 2706
Bsp1407I TGTACA 3 cut(s) 3752, 4062, 5100
Bsp19I CCATGG 3 cut(s) 334, 634, 784
BspANI GGCC 4 cut(s) 50, 874, 1068, 3273
BspDI ATCGAT 3 cut(s) 1152, 2895, 4100
BspEI TCCGGA 2 cut(s) 1309, 2706
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 2853
BspLI GGNNCC 7 cut(s) 232, 1176, 2762, 4915, 5131, 5132, 5358
BspMI ACCTGC 1 cut(s) 3387
BspPI GGATC 7 cut(s) 1288, 2223, 2755, 2768, 4654, 4867, 4912
BspQI GCTCTTC 1 cut(s) 2020
BspT104I TTCGAA 2 cut(s) 2177, 5137
BspTI CTTAAG 3 cut(s) 2612, 3911, 4264
BspTNI GGTCTC 2 cut(s) 2591, 3381
BsrBI CCGCTC 1 cut(s) 47
BsrDI GCAATG 5 cut(s) 2047, 2556, 3183, 3231, 4626
BsrGI TGTACA 3 cut(s) 3752, 4062, 5100
BsrI ACTGG 8 cut(s) 1421, 3349, 3448, 3808, 3895, 4134, 4373, 4598
BssNI GRCGYC 2 cut(s) 35, 1035
BssT1I CCWWGG 7 cut(s) 334, 634, 784, 1582, 1880, 1979, 5125
Bst6I CTCTTC 7 cut(s) 2020, 2559, 3561, 4315, 4714, 4719, 4737
BstACI GRCGYC 2 cut(s) 35, 1035
BstAFI CTTAAG 3 cut(s) 2612, 3911, 4264
BstAPI GCANNNNNTGC 2 cut(s) 311, 858
BstAUI TGTACA 3 cut(s) 3752, 4062, 5100
BstBAI YACGTR 2 cut(s) 3427, 4872
BstBI TTCGAA 2 cut(s) 2177, 5137
BstC8I GCNNGC 4 cut(s) 859, 1200, 2322, 3617
BstDSI CCRYGG 6 cut(s) 150, 334, 409, 634, 784, 4495
BstENI CCTNNNNNAGG 1 cut(s) 1581
BstFNI CGCG 1 cut(s) 56
BstH2I RGCGCY 2 cut(s) 606, 756
BstHHI GCGC 6 cut(s) 455, 605, 755, 3839, 4696, 5032
BstMAI GTCTC 8 cut(s) 707, 2591, 2940, 3381, 3784, 4739, 4775, 5182
BstNSI RCATGY 9 cut(s) 861, 1369, 1735, 2964, 3192, 3260, 3325, 3481, 4788
BstPAI GACNNNNGTC 1 cut(s) 2603
BstSFI CTRYAG 2 cut(s) 2239, 5172
BstSLI GKGCMC 2 cut(s) 188, 1481
BstUI CGCG 1 cut(s) 56
BstV2I GAAGAC 3 cut(s) 969, 1764, 1803
BstX2I RGATCY 7 cut(s) 1293, 1517, 2568, 2760, 4038, 4437, 4859
BstXI CCANNNNNNTGG 6 cut(s) 341, 416, 641, 791, 2316, 3344
BstYI RGATCY 7 cut(s) 1293, 1517, 2568, 2760, 4038, 4437, 4859
Bsu15I ATCGAT 3 cut(s) 1152, 2895, 4100
Bsu36I CCTNAGG 2 cut(s) 1342, 2982
BsuI GTATCC 1 cut(s) 4998
BsuRI GGCC 4 cut(s) 50, 874, 1068, 3273
BsuTUI ATCGAT 3 cut(s) 1152, 2895, 4100
BtgI CCRYGG 6 cut(s) 150, 334, 409, 634, 784, 4495
BtgZI GCGATG 1 cut(s) 814
BtrI CACGTC 1 cut(s) 2873
BtsI GCAGTG 1 cut(s) 2483
BtsIMutI CAGTG 6 cut(s) 399, 973, 2483, 3287, 3441, 4794
BveI ACCTGC 1 cut(s) 3387
Cac8I GCNNGC 4 cut(s) 859, 1200, 2322, 3617
CciI TCATGA 1 cut(s) 2853
CfoI GCGC 6 cut(s) 455, 605, 755, 3839, 4696, 5032
Cfr13I GGNCC 8 cut(s) 611, 1066, 1175, 2617, 2834, 4132, 4914, 5130
ClaI ATCGAT 3 cut(s) 1152, 2895, 4100
CseI GACGC 3 cut(s) 1024, 2827, 5282
CspCI CAANNNNNGTGG 2 cut(s) 544, 579
DraI TTTAAA 3 cut(s) 2415, 3936, 5376
DrdI GACNNNNNNGTC 3 cut(s) 34, 2371, 5174
DseDI GACNNNNNNGTC 3 cut(s) 34, 2371, 5174
EaeI YGGCCR 1 cut(s) 48
Eam1104I CTCTTC 7 cut(s) 2020, 2559, 3561, 4315, 4714, 4719, 4737
EarI CTCTTC 7 cut(s) 2020, 2559, 3561, 4315, 4714, 4719, 4737
EciI GGCGGA 1 cut(s) 1954
Ecl136II GAGCTC 1 cut(s) 1644
Eco130I CCWWGG 7 cut(s) 334, 634, 784, 1582, 1880, 1979, 5125
Eco147I AGGCCT 2 cut(s) 874, 3273
Eco24I GRGCYC 2 cut(s) 634, 1646
Eco31I GGTCTC 2 cut(s) 2591, 3381
Eco32I GATATC 2 cut(s) 2712, 5069
Eco47I GGWCC 7 cut(s) 611, 1175, 2617, 2834, 4132, 4914, 5130
Eco47III AGCGCT 2 cut(s) 604, 754
Eco53kI GAGCTC 1 cut(s) 1644
Eco72I CACGTG 1 cut(s) 4872
Eco81I CCTNAGG 2 cut(s) 1342, 2982
Eco88I CYCGRG 4 cut(s) 109, 127, 1133, 4641
EcoICRI GAGCTC 1 cut(s) 1644
EcoNI CCTNNNNNAGG 1 cut(s) 1581
EcoO109I RGGNCCY 3 cut(s) 1175, 2617, 5130
EcoRI GAATTC 1 cut(s) 545
EcoRV GATATC 2 cut(s) 2712, 5069
EcoT14I CCWWGG 7 cut(s) 334, 634, 784, 1582, 1880, 1979, 5125
EcoT22I ATGCAT 3 cut(s) 316, 863, 2495
EcoT38I GRGCYC 2 cut(s) 634, 1646
ErhI CCWWGG 7 cut(s) 334, 634, 784, 1582, 1880, 1979, 5125
Esp3I CGTCTC 1 cut(s) 4739
FalI AAGNNNNNCTT 4 cut(s) 2395, 2427, 5118, 5150
FauI CCCGC 1 cut(s) 5394
FauNDI CATATG 2 cut(s) 2167, 2254
FbaI TGATCA 1 cut(s) 4978
FblI GTMKAC 3 cut(s) 1548, 4147, 4353
FriOI GRGCYC 2 cut(s) 634, 1646
GlaI GCGC 6 cut(s) 454, 604, 754, 3838, 4695, 5031
GsuI CTGGAG 4 cut(s) 3974, 4247, 4342, 5343
HaeII RGCGCY 2 cut(s) 606, 756
HaeIII GGCC 4 cut(s) 50, 874, 1068, 3273
HapII CCGG 7 cut(s) 90, 234, 1310, 2080, 2707, 4034, 4451
HgaI GACGC 3 cut(s) 1024, 2827, 5282
HhaI GCGC 6 cut(s) 455, 605, 755, 3839, 4696, 5032
Hin1I GRCGYC 2 cut(s) 35, 1035
Hin6I GCGC 6 cut(s) 453, 603, 753, 3837, 4694, 5030
HinP1I GCGC 6 cut(s) 453, 603, 753, 3837, 4694, 5030
HindIII AAGCTT 4 cut(s) 2728, 3776, 3917, 4430
HpaI GTTAAC 1 cut(s) 3813
HpaII CCGG 7 cut(s) 90, 234, 1310, 2080, 2707, 4034, 4451
HphI GGTGA 9 cut(s) 104, 932, 2241, 2452, 2970, 4035, 4537, 4586, 4820
Hpy99I CGWCG 4 cut(s) 178, 181, 4249, 4840
HpyCH4IV ACGT 7 cut(s) 35, 346, 2872, 3426, 3510, 4835, 4871
HpySE526I ACGT 7 cut(s) 35, 346, 2872, 3426, 3510, 4835, 4871
Hsp92I GRCGYC 2 cut(s) 35, 1035
HspAI GCGC 6 cut(s) 453, 603, 753, 3837, 4694, 5030
KflI GGGWCCC 1 cut(s) 5130
Kpn2I TCCGGA 2 cut(s) 1309, 2706
Ksp22I TGATCA 1 cut(s) 4978
KspAI GTTAAC 1 cut(s) 3813
LguI GCTCTTC 1 cut(s) 2020
MaeII ACGT 7 cut(s) 35, 346, 2872, 3426, 3510, 4835, 4871
MbiI CCGCTC 1 cut(s) 47
MfeI CAATTG 1 cut(s) 1633
MflI RGATCY 7 cut(s) 1293, 1517, 2568, 2760, 4038, 4437, 4859
MhlI GDGCHC 5 cut(s) 188, 634, 1365, 1481, 1646
MlyI GAGTC 9 cut(s) 690, 765, 2507, 4235, 4349, 4430, 4805, 5099, 5162
MmeI TCCRAC 5 cut(s) 1698, 3241, 3828, 4190, 4636
Mph1103I ATGCAT 3 cut(s) 316, 863, 2495
MroI TCCGGA 2 cut(s) 1309, 2706
MroXI GAANNNNTTC 2 cut(s) 18, 3357
MslI CAYNNNNRTG 9 cut(s) 339, 414, 489, 639, 789, 2064, 2488, 2904, 4999
MspA1I CMGCKG 2 cut(s) 3374, 5389
MspCI CTTAAG 3 cut(s) 2612, 3911, 4264
MspI CCGG 7 cut(s) 90, 234, 1310, 2080, 2707, 4034, 4451
MunI CAATTG 1 cut(s) 1633
Mva1269I GAATGC 1 cut(s) 3412
MvnI CGCG 1 cut(s) 56
NciI CCSGG 1 cut(s) 4034
NcoI CCATGG 3 cut(s) 334, 634, 784
NdeI CATATG 2 cut(s) 2167, 2254
NlaIV GGNNCC 7 cut(s) 232, 1176, 2762, 4915, 5131, 5132, 5358
NmeAIII GCCGAG 3 cut(s) 653, 3636, 4725
NmuCI GTSAC 7 cut(s) 1368, 1745, 2481, 2645, 2976, 3000, 4808
NsiI ATGCAT 3 cut(s) 316, 863, 2495
NspI RCATGY 9 cut(s) 861, 1369, 1735, 2964, 3192, 3260, 3325, 3481, 4788
NspV TTCGAA 2 cut(s) 2177, 5137
OliI CACNNNNGTG 1 cut(s) 414
PaeI GCATGC 1 cut(s) 861
PaeR7I CTCGAG 2 cut(s) 109, 4641
PagI TCATGA 1 cut(s) 2853
PceI AGGCCT 2 cut(s) 874, 3273
PciI ACATGT 5 cut(s) 1365, 1731, 2960, 3256, 3477
PciSI GCTCTTC 1 cut(s) 2020
PcsI WCGNNNNNNNCGW 1 cut(s) 2749
PctI GAATGC 1 cut(s) 3412
PdmI GAANNNNTTC 2 cut(s) 18, 3357
PfeI GAWTC 8 cut(s) 1313, 1969, 2378, 3368, 3484, 3983, 4159, 5197
PflFI GACNNNGTC 3 cut(s) 699, 2998, 3218
PfoI TCCNGGA 3 cut(s) 2836, 4032, 4358
PleI GAGTC 9 cut(s) 690, 765, 2506, 4235, 4349, 4429, 4805, 5098, 5162
PmaCI CACGTG 1 cut(s) 4872
PmlI CACGTG 1 cut(s) 4872
PpsI GAGTC 9 cut(s) 690, 765, 2506, 4235, 4349, 4429, 4805, 5098, 5162
Ppu21I YACGTR 2 cut(s) 3427, 4872
PpuMI RGGWCCY 3 cut(s) 1175, 2617, 5130
PscI ACATGT 5 cut(s) 1365, 1731, 2960, 3256, 3477
PshAI GACNNNNGTC 1 cut(s) 2603
Psp124BI GAGCTC 1 cut(s) 1646
Psp5II RGGWCCY 3 cut(s) 1175, 2617, 5130
PspCI CACGTG 1 cut(s) 4872
PspN4I GGNNCC 7 cut(s) 232, 1176, 2762, 4915, 5131, 5132, 5358
PspPI GGNCC 8 cut(s) 611, 1066, 1175, 2617, 2834, 4132, 4914, 5130
PspPPI RGGWCCY 3 cut(s) 1175, 2617, 5130
PspXI VCTCGAGB 2 cut(s) 109, 4641
PsuI RGATCY 7 cut(s) 1293, 1517, 2568, 2760, 4038, 4437, 4859
PsyI GACNNNGTC 3 cut(s) 699, 2998, 3218
PvuII CAGCTG 1 cut(s) 3374
RseI CAYNNNNRTG 9 cut(s) 339, 414, 489, 639, 789, 2064, 2488, 2904, 4999
SacI GAGCTC 1 cut(s) 1646
SapI GCTCTTC 1 cut(s) 2020
Sau96I GGNCC 8 cut(s) 611, 1066, 1175, 2617, 2834, 4132, 4914, 5130
ScaI AGTACT 2 cut(s) 466, 4602
SchI GAGTC 9 cut(s) 690, 765, 2507, 4235, 4349, 4430, 4805, 5099, 5162
SduI GDGCHC 5 cut(s) 188, 634, 1365, 1481, 1646
SfcI CTRYAG 2 cut(s) 2239, 5172
Sfr274I CTCGAG 2 cut(s) 109, 4641
SfuI TTCGAA 2 cut(s) 2177, 5137
SinI GGWCC 7 cut(s) 611, 1175, 2617, 2834, 4132, 4914, 5130
SlaI CTCGAG 2 cut(s) 109, 4641
SmiMI CAYNNNNRTG 9 cut(s) 339, 414, 489, 639, 789, 2064, 2488, 2904, 4999
SpeI ACTAGT 3 cut(s) 59, 1028, 1775
SphI GCATGC 1 cut(s) 861
SseBI AGGCCT 2 cut(s) 874, 3273
SspI AATATT 3 cut(s) 3605, 3641, 3961
SstI GAGCTC 1 cut(s) 1646
StuI AGGCCT 2 cut(s) 874, 3273
StyI CCWWGG 7 cut(s) 334, 634, 784, 1582, 1880, 1979, 5125
TaiI ACGT 7 cut(s) 38, 349, 2875, 3429, 3513, 4838, 4874
TatI WGTACW 7 cut(s) 396, 464, 1728, 3752, 4062, 4600, 5100
TauI GCSGC 2 cut(s) 50, 4699
TfiI GAWTC 8 cut(s) 1313, 1969, 2378, 3368, 3484, 3983, 4159, 5197
TscAI CASTG 6 cut(s) 399, 973, 2490, 3294, 3448, 4801
TseFI GTSAC 7 cut(s) 1368, 1745, 2481, 2645, 2976, 3000, 4808
Tsp45I GTSAC 7 cut(s) 1368, 1745, 2481, 2645, 2976, 3000, 4808
TspGWI ACGGA 6 cut(s) 12, 426, 2037, 4754, 4906, 5328
TspRI CASTG 6 cut(s) 399, 973, 2490, 3294, 3448, 4801
Tth111I GACNNNGTC 3 cut(s) 699, 2998, 3218
Vha464I CTTAAG 3 cut(s) 2612, 3911, 4264
VneI GTGCAC 2 cut(s) 184, 1477
VpaK11BI GGWCC 7 cut(s) 611, 1175, 2617, 2834, 4132, 4914, 5130
XagI CCTNNNNNAGG 1 cut(s) 1581
XapI RAATTY 7 cut(s) 545, 2105, 2665, 3017, 3491, 3931, 5095
XbaI TCTAGA 1 cut(s) 2861
XceI RCATGY 9 cut(s) 861, 1369, 1735, 2964, 3192, 3260, 3325, 3481, 4788
XcmI CCANNNNNNNNNTGG 2 cut(s) 1589, 3766
XhoI CTCGAG 2 cut(s) 109, 4641
XmaJI CCTAGG 1 cut(s) 1880
XmiI GTMKAC 3 cut(s) 1548, 4147, 4353
XmnI GAANNNNTTC 2 cut(s) 18, 3357
ZraI GACGTC 1 cut(s) 36
ZrmI AGTACT 2 cut(s) 466, 4602
Zsp2I ATGCAT 3 cut(s) 316, 863, 2495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.