AT4G19500

MAP kinase kinase kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0027571
Physical Location & Seq
Forward (+)
10625562 .. 10630433
4872 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G19500.2

Sequence Viewer

Length: 3930 bp
ATGGCAGCTTCTTTTTGCGGCAGCCGGAGATACGATGTTTTCCCGAGCTTCAGTAAGGTAGATGTCCGCAGGTCATTCCTCGCGCATCTTCTCAAGGAGCTCGACCGCAGATTAATCAATACGTTCACAGATCATGGTATGGAGAGAAACCTCCCAATCGACGCTGAACTTTTATCGGCGATAGCAGAATCGAGGATCTCAATAGTCATCTTCTCTAAAAACTATGCTTCTTCCACGTGGTGCTTAGATGAATTGGTTGAGATCCACACGTGTTATAAGGAATTGGCTCAAATAGTGGTTCCGGTTTTCTTTAACGTACATCCTTCGCAAGTTAAAAAACAGACCGGAGAATTTGGTAAGGTTTTTGGAAAGACATGCAAAGGTAAACCAGAGAATCGGAAACTAAGATGGATGCAAGCTCTAGCAGCGGTAGCAAATATTGCTGGATATGATCTTCAGAACTGGCCTGATGAAGCTGTCATGATTGAGATGGTAGCTGACGATGTTTCGAAAAAACTTTTTAAATCATCGAATGATTTCAGTGATATCGTCGGGATTGAAGCTCATTTAGAGGCAATGAGTTCAATATTGCGCTTGAAATCTGAGAAAGCTAGAATGGTCGGGATTTCGGGGCCTTCAGGGATTGGTAAGACTACCATCGCAAAAGCTCTTTTCAGTAAACTCTCTCCCCAATTCCACCTTCGTGCTTTCGTTACTTATAAAAGAACCAACCAGGACGACTATGACATGAAGTTGTGTTGGATAGAAAAATTTCTGTCAGAAATTCTTGGTCAAAAGGACTTGAAGGTTTTGGATTTAGGTGCGGTGGAACAAAGTCTAATGCACAAGAAAGTTCTTATCATTCTTGACGATGTAGATGATCTTGAGCTATTAAAGACCTTGGTGGGACAAACTGGATGGTTCGGGTTTGGAAGCAGAATTGTTGTGATCACTCAGGATAGGCAGCTTCTCAAGGCTCATGATATTAACCTCATATATGAGGTGGCCTTCCCATCTGCCCATCTTGCTCTTGAGATTTTCTGCCAATCTGCTTTTGGGAAAATATATCCACCATCTGATTTTAGAGAACTCTCTGTTGAATTTGCATATCTTGCCGGCAATCTTCCTTTGGATCTTAGGGTCTTGGGTTTGGCCATGAAAGGAAAGCACAGGGAGGAGTGGATAGAGATGCTGCCTAGGCTCCGAAATGATTTGGACGGGAAATTTAAGAAAACATTGAGAAATTACCTGCCTGTGATACGGAAGCGCGTTTCCAATGAAGAAGGGGGCCGTGAGAAATTGAAAAAGGGAAATAAAAAGTTGGATTTGGATGAGGAGTTTCCTGGTGGAGAAATTTACAGTGATGAGATACCTTCGCCAACATCTAACTGGAAAGATACAGATGACTTTGATTCAGGGGACATCATTCCAATCATTGCAGACAAATCTACTACTATAATTCCCAACAGGAGGCACTCGAACGATGACTGGTGTTCTTTCTGTGAGTTCCTCAGAAACCGTATACCCCCGTTGAATCCCTTTAAATGTAGTGCCAATGATGTCATTGATTTTCTTCGCACACGGCAGGTTTTAGGCAGCACTGAGGCTCTCGTTGACCGCCTCATTTTCAGTAGTGAGGCATTTGGCATAAAACCTGAGGAAAACCCTTTTCGCAGCCAAGCTGTAACATCGTACTTGAAGGCGGCCAGGGATATGACACGAGAAAAAGAATGCATACTTGTGTTTTCGTGCCACGACAACCTTGATGTAGATGAAACATCTTTCATTGAAGCCATCTCAAAAGAATTGCACAAGCAGGGGTTCATCCCTTTGACATATAATCTTTTGGGCAGAGAGAACCTCGATGAGGAGATGTTATACGGATCTAGAGTCGGTATCATGATACTTTCAAGTAGTTATGTTTCTTCTAGACAGTCCCTGGATCACCTGGTTGCAGTTATGGAGCATTGGAAAACAACAGACCTTGTAATTATTCCTATATATTTTAAAGTAAGACTTTCAGACATTTGTGGGTTGAAAGGCAGGTTTGAAGCAGCGTTTCTGCAGCTTCATATGTCTCTCCAGGAAGACAGAGTTCAGAAATGGAAGGCGGCTATGTCTGAAATAGTGTCCATCGGTGGACATGAATGGACCAAGGGAAGTCAGTTTATTCTTGCCGAGGAAGTTGTAAGAAATGCATCCTTAAGGCTATATCTGAAAAGTAGCAAGAATCTGCTTGGAATCTTAGCGTTGTTAAATCACTCCCAGTCTACAGACGTGGAAATTATGGGAATCTGGGGTATAGCAGGAATAGGTAAGACATCGATTGCAAGAGAAATATTTGAATTACATGCTCCACATTATGATTTCTGTTACTTCCTGCAAGACTTTCATCTAATGTGTCAGATGAAAAGGCCGAGGCAATTGCGTGAAGATTTTATCTCAAAATTGTTTGGGGAAGAAAAAGGTCTAGGTGCTAGTGATGTAAAGCCAAGTTTCATGAGGGACTGGTTCCATAAAAAAACGATTCTTCTCGTTCTTGATGACGTGAGTAATGCCAGAGATGCAGAAGCTGTAATCGGAGGGTTTGGCTGGTTTTCTCATGGACACAGAATCATCTTAACCTCTAGGAGTAAACAAGTTCTTGTACAGTGTAAGGTTAAAAAGCCATACGAGATCCAAAAATTAAGCGATTTTGAATCGTTTCGTCTCTGCAAACAATATTTGGATGGCGAAAATCCGGTCATCTCTGAGCTTATCAGCTGCAGTAGTGGTATTCCATTGGCTCTCAAACTTTTAGTTTCCTCTGTATCAAAGCAGTATATAACGAATATGAAAGACCATCTCCAAAGCTTGAGGAAAGATCCTCCTACTCAGATTCAAGAAGCATTTCGGAGAAGTTTTGATGGACTAGATGAAAACGAGAAAAACATATTTTTGGATCTTGCATGTTTTTTCAGGGGGCAGAGCAAAGATTATGCGGTGCTATTACTTGATGCTTGTGGTTTTTTTACATATATGGGAATCTGTGAGCTCATTGACGAGTCACTCATTAGCCTTGTAGACAACAAGATAGAGATGCCTATTCCTTTTCAAGACATGGGCCGAATTATTGTTCATGAAGAAGATGAGGATCCATGTGAACGTAGCAGATTGTGGGACTCGAAGGACATCGTTGATGTTTTGACAAACAATTCAGGAACAGAAGCAATTGAGGGCATCTTCCTGGATGCGTCTGACTTGACCTGCGAGCTTAGTCCTACTGTGTTTGGTAAGATGTATAATCTTAGATTGCTGAAGTTCTATTGTTCAACCTCTGGGAACCAGTGCAAGCTTACTCTACCTCACGGCCTAGACACTTTGCCTGATGAGCTAAGTCTACTTCACTGGGAGAATTACCCTCTGGTTTACTTGCCTCAGAAATTTAATCCTGTGAACCTTGTAGAGTTAAACATGCCTTATAGCAACATGGAGAAGTTGTGGGAAGGAAAGAAAAATCTCGAGAAGCTAAAGAACATCAAACTGAGTCACTCCAGAGAATTAACTGATATCCTGATGTTATCAGAAGCCCTGAACCTGGAACACATTGATCTCGAAGGGTGTACGAGTCTGATTGATGTTAGCATGTCTATTCCTTGTTGTGGGAAGCTTGTTTCCTTGAATATGAAAGACTGTTCTCGTTTGCGAAGTCTGCCTTCTATGGTTGATTTAACAACTCTCAAGCTTCTTAATTTGTCTGGCTGCTCAGAATTTGAGGATATTCAGGATTTTGCACCAAACCTGGAAGAGATATATCTAGCTGGGACATCCATTAGAGAGCTTCCGTTGTCAATCAGGAATCTCACTGAACTTGTTACGCTAGATCTGGAGAACTGCGAAAGGCTTCAGGAAATGCCGAGTCTTCCGGTGGAAATAATCAGGAGAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 DUF4116 HET LRR_3 NB-ARC Pkinase TIR

Protein Analysis

1309

Amino Acids

148.97

Weight (kDa)

5.72

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 68 - 210 2.2e-16 TIR domain
NB-ARC PF00931 233 - 385 9.9e-16 NB-ARC domain
WHD_ROQ1 PF23282 449 - 518 3.4e-15 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 623 - 642 2.7e-10 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0027571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19500 AT4G19500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 276, 720
Acc36I ACCTGC 5 cut(s) 60, 1257, 1576, 2034, 3254
AccI GTMKAC 4 cut(s) 1522, 2270, 3063, 3379
AccII CGCG 2 cut(s) 83, 1269
AciI CCGC 9 cut(s) 18, 67, 106, 428, 824, 1618, 1703, 2111, 2981
AcoI YGGCCR 2 cut(s) 1152, 1704
AcsI RAATTY 8 cut(s) 350, 770, 783, 1100, 1223, 1353, 3422, 3749
AcuI CTGAAG 5 cut(s) 34, 440, 621, 3317, 3869
AcvI CACGTG 2 cut(s) 237, 270
AdeI CACNNNGTG 1 cut(s) 240
AfaI GTAC 4 cut(s) 318, 1694, 2649, 3604
AfiI CCNNNNNNNGG 4 cut(s) 1470, 1867, 3577, 3641
AflII CTTAAG 1 cut(s) 2203
AflIII ACRYGT 2 cut(s) 267, 269
AjiI CACGTC 2 cut(s) 2278, 2548
AjnI CCWGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
AjuI GAANNNNNNNTTGG 2 cut(s) 684, 716
AleI CACNNNNGTG 1 cut(s) 702
Alw21I GWGCWC 2 cut(s) 102, 3036
Alw26I GTCTC 2 cut(s) 2082, 2714
AlwNI CAGNNNCTG 2 cut(s) 1939, 2573
Ama87I CYCGRG 2 cut(s) 43, 3500
AoxI GGCC 9 cut(s) 464, 632, 1005, 1152, 1288, 1704, 2414, 3103, 3349
ApoI RAATTY 8 cut(s) 350, 770, 783, 1100, 1223, 1353, 3422, 3749
ArsI GACNNNNNNTTYG 2 cut(s) 2727, 2759
AseI ATTAAT 1 cut(s) 113
Asp700I GAANNNNTTC 5 cut(s) 536, 771, 2703, 2889, 3882
AspA2I CCTAGG 1 cut(s) 1196
AspLEI GCGC 3 cut(s) 85, 594, 1269
AspS9I GGNCC 4 cut(s) 632, 1288, 2151, 3103
AsuHPI GGTGA 1 cut(s) 1937
AsuII TTCGAA 1 cut(s) 509
AvaI CYCGRG 2 cut(s) 43, 3500
AvaII GGWCC 1 cut(s) 2151
AvrII CCTAGG 1 cut(s) 1196
AxyI CCTNAGG 1 cut(s) 1656
BalI TGGCCA 1 cut(s) 1154
BamHI GGATCC 1 cut(s) 3133
BanII GRGCYC 2 cut(s) 102, 3036
BauI CACGAG 1 cut(s) 1719
BbrPI CACGTG 2 cut(s) 237, 270
BbsI GAAGAC 2 cut(s) 2094, 3893
Bbv12I GWGCWC 2 cut(s) 102, 3036
BceAI ACGGC 3 cut(s) 1275, 1598, 3364
BcgI CGANNNNNNTGC 2 cut(s) 2407, 2441
BciT130I CCWGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
BclI TGATCA 1 cut(s) 948
BcoDI GTCTC 2 cut(s) 2082, 2714
BfmI CTRYAG 3 cut(s) 2063, 2271, 2764
BfrI CTTAAG 1 cut(s) 2203
BfuAI ACCTGC 5 cut(s) 60, 1257, 1576, 2034, 3254
BglII AGATCT 1 cut(s) 3862
BlnI CCTAGG 1 cut(s) 1196
Bme1390I CCNGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
Bme18I GGWCC 1 cut(s) 2151
BmeT110I CYCGRG 2 cut(s) 43, 3500
BmgBI CACGTC 2 cut(s) 2278, 2548
BmgT120I GGNCC 4 cut(s) 632, 1288, 2151, 3103
BmiI GGNNCC 7 cut(s) 300, 633, 1202, 1289, 2513, 3135, 3323
BmrFI CCNGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
BmrI ACTGGG 2 cut(s) 2260, 3397
BmsI GCATC 9 cut(s) 94, 402, 1179, 2207, 2554, 2986, 3069, 3220, 3228
BmuI ACTGGG 2 cut(s) 2260, 3397
BpiI GAAGAC 2 cut(s) 2094, 3893
BplI GAGNNNNNCTC 2 cut(s) 1845, 1877
BpmI CTGGAG 3 cut(s) 2066, 3517, 3887
Bpu14I TTCGAA 1 cut(s) 509
BpuEI CTTGAG 6 cut(s) 77, 905, 956, 1052, 2875, 3704
Bsa29I ATCGAT 1 cut(s) 2324
BsaAI YACGTR 2 cut(s) 237, 270
BsaBI GATNNNNATC 1 cut(s) 3132
BsaJI CCNNGG 7 cut(s) 900, 1196, 1707, 1938, 2154, 2178, 2417
BsaWI WCCGGW 4 cut(s) 301, 344, 2740, 3904
BsaXI ACNNNNNCTCC 2 cut(s) 3464, 3494
Bsc4I CCNNNNNNNGG 4 cut(s) 1470, 1867, 3577, 3641
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 8 cut(s) 467, 919, 1394, 1493, 2266, 2513, 3325, 3392
Bse21I CCTNAGG 1 cut(s) 1656
Bse3DI GCAATG 2 cut(s) 582, 1434
Bse8I GATNNNNATC 1 cut(s) 3132
BseBI CCWGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
BseCI ATCGAT 1 cut(s) 2324
BseDI CCNNGG 7 cut(s) 900, 1196, 1707, 1938, 2154, 2178, 2417
BseGI GGATG 9 cut(s) 319, 417, 923, 1336, 1824, 2198, 2734, 3235, 3806
BseJI GATNNNNATC 1 cut(s) 3132
BseLI CCNNNNNNNGG 4 cut(s) 1470, 1867, 3577, 3641
BseMI GCAATG 2 cut(s) 582, 1434
BseNI ACTGG 8 cut(s) 467, 919, 1394, 1493, 2266, 2513, 3325, 3392
BseRI GAGGAG 3 cut(s) 1190, 1349, 1883
BseYI CCCAGC 1 cut(s) 3800
Bsh1236I CGCG 2 cut(s) 83, 1269
Bsh1285I CGRYCG 1 cut(s) 106
BshFI GGCC 9 cut(s) 466, 634, 1007, 1154, 1290, 1706, 2416, 3105, 3351
BshVI ATCGAT 1 cut(s) 2324
BsiEI CGRYCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 102, 3036
BsiHKCI CYCGRG 2 cut(s) 43, 3500
BsiSI CCGG 6 cut(s) 25, 302, 345, 1116, 2741, 3905
BslFI GGGAC 6 cut(s) 921, 1433, 1921, 2519, 3173, 3817
BslI CCNNNNNNNGG 4 cut(s) 1470, 1867, 3577, 3641
BsmAI GTCTC 2 cut(s) 2082, 2714
BsmBI CGTCTC 1 cut(s) 2714
BsmFI GGGAC 6 cut(s) 921, 1433, 1921, 2519, 3173, 3817
BsmI GAATGC 1 cut(s) 1736
BsnI GGCC 9 cut(s) 466, 634, 1007, 1154, 1290, 1706, 2416, 3105, 3351
BsoBI CYCGRG 2 cut(s) 43, 3500
Bsp119I TTCGAA 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 102, 3036
Bsp1407I TGTACA 1 cut(s) 2647
BspACI CCGC 9 cut(s) 18, 67, 106, 428, 824, 1618, 1703, 2111, 2981
BspANI GGCC 9 cut(s) 466, 634, 1007, 1154, 1290, 1706, 2416, 3105, 3351
BspDI ATCGAT 1 cut(s) 2324
BspFNI CGCG 2 cut(s) 83, 1269
BspHI TCATGA 5 cut(s) 480, 979, 1899, 2499, 3118
BspLI GGNNCC 7 cut(s) 300, 633, 1202, 1289, 2513, 3135, 3323
BspMAI CTGCAG 2 cut(s) 2067, 2768
BspMI ACCTGC 5 cut(s) 60, 1257, 1576, 2034, 3254
BspT104I TTCGAA 1 cut(s) 509
BspTI CTTAAG 1 cut(s) 2203
BsrDI GCAATG 2 cut(s) 582, 1434
BsrFI RCCGGY 1 cut(s) 1115
BsrGI TGTACA 1 cut(s) 2647
BsrI ACTGG 8 cut(s) 467, 919, 1394, 1493, 2266, 2513, 3325, 3392
BssAI RCCGGY 1 cut(s) 1115
BssECI CCNNGG 7 cut(s) 900, 1196, 1707, 1938, 2154, 2178, 2417
BssNAI GTATAC 1 cut(s) 1523
BssSI CACGAG 1 cut(s) 1719
BssT1I CCWWGG 3 cut(s) 900, 1196, 2154
Bst1107I GTATAC 1 cut(s) 1523
Bst2BI CACGAG 1 cut(s) 1719
Bst2UI CCWGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
Bst4CI ACNGT 6 cut(s) 1361, 1520, 1935, 2652, 3265, 3674
Bst6I CTCTTC 1 cut(s) 3780
BstAFI CTTAAG 1 cut(s) 2203
BstAPI GCANNNNNTGC 2 cut(s) 440, 1112
BstAUI TGTACA 1 cut(s) 2647
BstBAI YACGTR 2 cut(s) 237, 270
BstBI TTCGAA 1 cut(s) 509
BstC8I GCNNGC 4 cut(s) 417, 1117, 3251, 3332
BstENI CCTNNNNNAGG 1 cut(s) 1865
BstF5I GGATG 9 cut(s) 319, 417, 923, 1336, 1824, 2198, 2734, 3235, 3806
BstFNI CGCG 2 cut(s) 83, 1269
BstHHI GCGC 3 cut(s) 85, 594, 1269
BstMAI GTCTC 2 cut(s) 2082, 2714
BstMCI CGRYCG 1 cut(s) 106
BstMWI GCNNNNNNNGC 9 cut(s) 425, 431, 440, 1025, 1112, 1198, 2564, 3370, 3691
BstNI CCWGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
BstNSI RCATGY 5 cut(s) 378, 2354, 2952, 3457, 3628
BstSCI CCNGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
BstSFI CTRYAG 3 cut(s) 2063, 2271, 2764
BstUI CGCG 2 cut(s) 83, 1269
BstV2I GAAGAC 2 cut(s) 2094, 3893
BstX2I RGATCY 9 cut(s) 195, 261, 1132, 1883, 2676, 2863, 2941, 3133, 3862
BstYI RGATCY 9 cut(s) 195, 261, 1132, 1883, 2676, 2863, 2941, 3133, 3862
BstZ17I GTATAC 1 cut(s) 1523
Bsu15I ATCGAT 1 cut(s) 2324
Bsu36I CCTNAGG 1 cut(s) 1656
BsuRI GGCC 9 cut(s) 466, 634, 1007, 1154, 1290, 1706, 2416, 3105, 3351
BsuTUI ATCGAT 1 cut(s) 2324
BtgZI GCGATG 1 cut(s) 643
BtrI CACGTC 2 cut(s) 2278, 2548
BtsCI GGATG 9 cut(s) 319, 417, 923, 1336, 1824, 2198, 2734, 3235, 3806
BtsIMutI CAGTG 7 cut(s) 547, 1366, 1599, 2657, 3332, 3385, 3843
BveI ACCTGC 5 cut(s) 60, 1257, 1576, 2034, 3254
Cac8I GCNNGC 4 cut(s) 417, 1117, 3251, 3332
CaiI CAGNNNCTG 2 cut(s) 1939, 2573
CciI TCATGA 5 cut(s) 480, 979, 1899, 2499, 3118
CfoI GCGC 3 cut(s) 85, 594, 1269
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 4 cut(s) 632, 1288, 2151, 3103
ClaI ATCGAT 1 cut(s) 2324
CseI GACGC 2 cut(s) 170, 3222
CsiI ACCWGGT 1 cut(s) 1947
Csp6I GTAC 4 cut(s) 317, 1693, 2648, 3603
CviQI GTAC 4 cut(s) 317, 1693, 2648, 3603
DraI TTTAAA 3 cut(s) 523, 1543, 2008
DraIII CACNNNGTG 1 cut(s) 240
EaeI YGGCCR 2 cut(s) 1152, 1704
Eam1104I CTCTTC 1 cut(s) 3780
EarI CTCTTC 1 cut(s) 3780
Ecl136II GAGCTC 2 cut(s) 100, 3034
Eco130I CCWWGG 3 cut(s) 900, 1196, 2154
Eco24I GRGCYC 2 cut(s) 102, 3036
Eco32I GATATC 2 cut(s) 547, 3550
Eco47I GGWCC 1 cut(s) 2151
Eco53kI GAGCTC 2 cut(s) 100, 3034
Eco57I CTGAAG 5 cut(s) 34, 440, 621, 3317, 3869
Eco72I CACGTG 2 cut(s) 237, 270
Eco81I CCTNAGG 1 cut(s) 1656
Eco88I CYCGRG 2 cut(s) 43, 3500
EcoICRI GAGCTC 2 cut(s) 100, 3034
EcoNI CCTNNNNNAGG 1 cut(s) 1865
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
EcoRV GATATC 2 cut(s) 547, 3550
EcoT14I CCWWGG 3 cut(s) 900, 1196, 2154
EcoT22I ATGCAT 2 cut(s) 1736, 2200
EcoT38I GRGCYC 2 cut(s) 102, 3036
ErhI CCWWGG 3 cut(s) 900, 1196, 2154
Esp3I CGTCTC 1 cut(s) 2714
FalI AAGNNNNNCTT 4 cut(s) 2001, 2033, 3679, 3711
FaqI GGGAC 6 cut(s) 921, 1433, 1921, 2519, 3173, 3817
FauNDI CATATG 1 cut(s) 2073
FbaI TGATCA 1 cut(s) 948
FblI GTMKAC 4 cut(s) 1522, 2270, 3063, 3379
FokI GGATG 9 cut(s) 306, 424, 930, 1343, 1811, 2185, 2741, 3242, 3793
FriOI GRGCYC 2 cut(s) 102, 3036
GlaI GCGC 3 cut(s) 84, 593, 1268
GsaI CCCAGC 1 cut(s) 3804
GsuI CTGGAG 3 cut(s) 2066, 3517, 3887
HaeIII GGCC 9 cut(s) 466, 634, 1007, 1154, 1290, 1706, 2416, 3105, 3351
HapII CCGG 6 cut(s) 25, 302, 345, 1116, 2741, 3905
HgaI GACGC 2 cut(s) 170, 3222
HhaI GCGC 3 cut(s) 85, 594, 1269
Hin6I GCGC 3 cut(s) 83, 592, 1267
HinP1I GCGC 3 cut(s) 83, 592, 1267
HincII GTYRAC 1 cut(s) 1615
HindII GTYRAC 1 cut(s) 1615
HindIII AAGCTT 4 cut(s) 2851, 3332, 3647, 3722
HpaII CCGG 6 cut(s) 25, 302, 345, 1116, 2741, 3905
HphI GGTGA 1 cut(s) 1937
Hpy99I CGWCG 2 cut(s) 164, 554
HpyCH4III ACNGT 6 cut(s) 1361, 1520, 1935, 2652, 3265, 3674
HpyCH4IV ACGT 7 cut(s) 122, 236, 269, 315, 2277, 2547, 3145
HpyF10VI GCNNNNNNNGC 9 cut(s) 425, 431, 440, 1025, 1112, 1198, 2564, 3370, 3691
HpySE526I ACGT 7 cut(s) 122, 236, 269, 315, 2277, 2547, 3145
HspAI GCGC 3 cut(s) 83, 592, 1267
KroI GCCGGC 1 cut(s) 1115
KroNI GCCGGC 1 cut(s) 1117
Ksp22I TGATCA 1 cut(s) 948
LmnI GCTCC 4 cut(s) 97, 1206, 1963, 2359
LweI GCATC 9 cut(s) 94, 402, 1179, 2207, 2554, 2986, 3069, 3220, 3228
MabI ACCWGGT 1 cut(s) 1947
MaeII ACGT 7 cut(s) 122, 236, 269, 315, 2277, 2547, 3145
MaeIII GTNAC 6 cut(s) 712, 1684, 2372, 3045, 3527, 3853
MfeI CAATTG 2 cut(s) 2423, 3210
MflI RGATCY 9 cut(s) 195, 261, 1132, 1883, 2676, 2863, 2941, 3133, 3862
MhlI GDGCHC 2 cut(s) 102, 3036
MlsI TGGCCA 1 cut(s) 1154
MluNI TGGCCA 1 cut(s) 1154
MlyI GAGTC 6 cut(s) 1899, 3053, 3155, 3535, 3616, 3907
MmeI TCCRAC 2 cut(s) 740, 1302
Mox20I TGGCCA 1 cut(s) 1154
Mph1103I ATGCAT 2 cut(s) 1736, 2200
MroNI GCCGGC 1 cut(s) 1115
MroXI GAANNNNTTC 5 cut(s) 536, 771, 2703, 2889, 3882
MscI TGGCCA 1 cut(s) 1154
MslI CAYNNNNRTG 2 cut(s) 702, 1739
Msp20I TGGCCA 1 cut(s) 1154
MspA1I CMGCKG 2 cut(s) 428, 2763
MspCI CTTAAG 1 cut(s) 2203
MspI CCGG 6 cut(s) 25, 302, 345, 1116, 2741, 3905
MspR9I CCNGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
MunI CAATTG 2 cut(s) 2423, 3210
Mva1269I GAATGC 1 cut(s) 1736
MvaI CCWGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
MvnI CGCG 2 cut(s) 83, 1269
MwoI GCNNNNNNNGC 9 cut(s) 425, 431, 440, 1025, 1112, 1198, 2564, 3370, 3691
NaeI GCCGGC 1 cut(s) 1117
NdeI CATATG 1 cut(s) 2073
NgoMIV GCCGGC 1 cut(s) 1115
NlaIV GGNNCC 7 cut(s) 300, 633, 1202, 1289, 2513, 3135, 3323
NmeAIII GCCGAG 3 cut(s) 2203, 2442, 3921
NmuCI GTSAC 2 cut(s) 3045, 3527
NsiI ATGCAT 2 cut(s) 1736, 2200
NspI RCATGY 5 cut(s) 378, 2354, 2952, 3457, 3628
NspV TTCGAA 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 702
PaeR7I CTCGAG 1 cut(s) 3500
PagI TCATGA 5 cut(s) 480, 979, 1899, 2499, 3118
PctI GAATGC 1 cut(s) 1736
PdiI GCCGGC 1 cut(s) 1117
PdmI GAANNNNTTC 5 cut(s) 536, 771, 2703, 2889, 3882
PfoI TCCNGGA 2 cut(s) 2082, 3225
PleI GAGTC 6 cut(s) 1898, 3052, 3155, 3534, 3615, 3906
PmaCI CACGTG 2 cut(s) 237, 270
PmlI CACGTG 2 cut(s) 237, 270
PpsI GAGTC 6 cut(s) 1898, 3052, 3155, 3534, 3615, 3906
Ppu21I YACGTR 2 cut(s) 237, 270
PshBI ATTAAT 1 cut(s) 113
PsiI TTATAA 2 cut(s) 276, 720
Psp124BI GAGCTC 2 cut(s) 102, 3036
Psp6I CCWGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
PspCI CACGTG 2 cut(s) 237, 270
PspFI CCCAGC 1 cut(s) 3800
PspGI CCWGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
PspN4I GGNNCC 7 cut(s) 300, 633, 1202, 1289, 2513, 3135, 3323
PspPI GGNCC 4 cut(s) 632, 1288, 2151, 3103
PstI CTGCAG 2 cut(s) 2067, 2768
PstNI CAGNNNCTG 2 cut(s) 1939, 2573
PsuI RGATCY 9 cut(s) 195, 261, 1132, 1883, 2676, 2863, 2941, 3133, 3862
PvuII CAGCTG 1 cut(s) 2763
RsaI GTAC 4 cut(s) 318, 1694, 2649, 3604
RsaNI GTAC 4 cut(s) 317, 1693, 2648, 3603
RseI CAYNNNNRTG 2 cut(s) 702, 1739
SacI GAGCTC 2 cut(s) 102, 3036
Sau96I GGNCC 4 cut(s) 632, 1288, 2151, 3103
SchI GAGTC 6 cut(s) 1899, 3053, 3155, 3535, 3616, 3907
ScrFI CCNGG 9 cut(s) 734, 1344, 1708, 1940, 1949, 2084, 3227, 3578, 3782
SduI GDGCHC 2 cut(s) 102, 3036
SexAI ACCWGGT 1 cut(s) 1947
SfaNI GCATC 9 cut(s) 94, 402, 1179, 2207, 2554, 2986, 3069, 3220, 3228
SfcI CTRYAG 3 cut(s) 2063, 2271, 2764
Sfr274I CTCGAG 1 cut(s) 3500
SfuI TTCGAA 1 cut(s) 509
SinI GGWCC 1 cut(s) 2151
SlaI CTCGAG 1 cut(s) 3500
SmiMI CAYNNNNRTG 2 cut(s) 702, 1739
SmlI CTYRAG 8 cut(s) 92, 884, 971, 1031, 2203, 2854, 3500, 3719
SmoI CTYRAG 8 cut(s) 92, 884, 971, 1031, 2203, 2854, 3500, 3719
SsiI CCGC 9 cut(s) 18, 67, 106, 428, 824, 1618, 1703, 2111, 2981
SspI AATATT 4 cut(s) 439, 588, 2340, 2723
SstI GAGCTC 2 cut(s) 102, 3036
StyD4I CCNGG 9 cut(s) 732, 1342, 1706, 1938, 1947, 2082, 3225, 3576, 3780
StyI CCWWGG 3 cut(s) 900, 1196, 2154
TaaI ACNGT 6 cut(s) 1361, 1520, 1935, 2652, 3265, 3674
TaiI ACGT 7 cut(s) 125, 239, 272, 318, 2280, 2550, 3148
TatI WGTACW 1 cut(s) 2647
TauI GCSGC 3 cut(s) 21, 1706, 2114
TscAI CASTG 7 cut(s) 547, 1366, 1606, 2657, 3332, 3392, 3850
TseFI GTSAC 2 cut(s) 3045, 3527
Tsp45I GTSAC 2 cut(s) 3045, 3527
TspGWI ACGGA 3 cut(s) 1276, 1896, 3813
TspRI CASTG 7 cut(s) 547, 1366, 1606, 2657, 3332, 3392, 3850
Vha464I CTTAAG 1 cut(s) 2203
VpaK11BI GGWCC 1 cut(s) 2151
VspI ATTAAT 1 cut(s) 113
XagI CCTNNNNNAGG 1 cut(s) 1865
XapI RAATTY 8 cut(s) 350, 770, 783, 1100, 1223, 1353, 3422, 3749
XbaI TCTAGA 2 cut(s) 1886, 1928
XceI RCATGY 5 cut(s) 378, 2354, 2952, 3457, 3628
XcmI CCANNNNNNNNNTGG 2 cut(s) 1052, 1386
XhoI CTCGAG 1 cut(s) 3500
XmaJI CCTAGG 1 cut(s) 1196
XmiI GTMKAC 4 cut(s) 1522, 2270, 3063, 3379
XmnI GAANNNNTTC 5 cut(s) 536, 771, 2703, 2889, 3882
Zsp2I ATGCAT 2 cut(s) 1736, 2200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.