AT4G20450

leucine-rich repeat protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0027168
Physical Location & Seq
Reverse (-)
11023918 .. 11029090
5173 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20450.2

Sequence Viewer

Length: 2697 bp
ATGGAAGGTATTCACAAGCTTATTTTCTTGGCCCTAATATGGATTTTTTTGATAACTAATATCGTTGATGCTCAAGATCAACAAGGATTCATCAGCTTAGATTGCGGAATGCCCCGTAACGAGTCTAGTTATACCGACGAGTCCACCGGTTTGAATTTCTCATCCGACGCTGATTTTATTAGCAGCGGAAAAAGTGGTACCATCAAGACCGAAGACTCTGATTCTGGCGTGAAATACATAAAGCCATATAAGCAGCTTAGGTATTTTCCTGAAGGAGCACGAAACTGCTATAATCTGACTGTCATGCAGGGCACACATTATCTTATCAGAGCCGTGTTCGTATATGGAAATTATGACCTCAAACAAAGACCAAAATTTGATCTATATCTTGGCCCCAATTTCTGGACAACTATAAATTTGCAAGATCCTTCGGGTGGTTTCTATTATCGGATTTGGTTGCAAGATGGTACCGTAGAGGAGGTAATCCACATGCCAAAGTCAAACAATTTGGATATTTGTTTGGTTAAGACAGGAACAACGACGCCATTCATATCATCCTTGGAACTACGTCCCTTGAGAGATGACACCTATACTACTACAACCGGCTCTCTAAAGCTAATCTCCCGCTGGTATTTCAGGAAGCCTTTCCCCACTCTTGAATCCATCATACGACATCCAGATGATGTCCATGACCGTCTATGGGACGTATACCATGCGGATGAAGAATGGACAGATATAAACACCACCACCCCTGTAAATACAACTGTTAATGCCTTTGATCTGCCACAAGCTATAATTTCAAAGGCTTCCATACCCCAAGTTGCAAGTGATACATGGAGCACAACCTGGTCGATACAAAACCCGGACGACGACGTTCACGTGTACCTTCACTTTGCCGAGATCCAAGCCTTGAAGCCCAGTGATACCAGAGAGTTTAGTATCTTGTGGAATAAAAACACAATCATTAGGGATTACTACAGTCCTCTAGAGTTCATGGCTGACACTGTACCTATAAGAACCTCGAGTAAATGCGGTGACGATGGTTTCTGCTCCCTAGATCTCACAAGAACTAAGTCGTCAACTCTTCCACCATATTGTAATGCGATGGAGGTTTTCGGGTTACTCCAGCTTCTTCAGACCGAAACAGATGAAAACGACGTCACTACATTGAAGAACATTCAAGCCACTTATAGAATACAAAAAACGAACTGGCAAGGAGATCCATGTGTACCTATACAGTTTATATGGACTGGCCTAAACTGTAGCAACATGTTTCCGTCTATTCCACCAAGAATCACTTCCATAGACTTTTCTAACTTTGGATTAAATGGGACCATAACAAGTGATATACAATATCTTAACCAGCTACAGAAATTGGACCTGTCAAATAACAACTTGACGGGAAAGGTGCCTGAATTTCTCGCCAAAATGAAGTTGTTGACATTCATAAACTTGAGTGGGAACAATCTTAGCGGCTCAATTCCTCAAAGCCTTCTCAACATGGAAAAGAATGGGCTAATAACGTTGCTGTATAATGGAAATAACCTCTGTTTGGATCCATCATGCGAAAGTGAAACTGGTCCTGGGAATAACAAAAAGAAATTGCTAGTACCTATTCTTGCATCAGCTGCTTCTGTGGGAATTATTATAGCTGTGCTTCTTCTCGTCAACATTCTCTTACTTAGAAAGAAGAAGCCATCCAAAGCTTCACGCTCATCAATGGTGGCGAACAAGAGAAGCTATACTTATGAAGAGGTTGCGGTAATTACAAACAATTTTGAGAGACCTCTTGGCGAAGGAGGATTTGGAGTCGTGTACCACGGTAATGTAAATGATAACGAACAAGTGGCTGTAAAGGTGCTCTCTGAATCCTCAGCTCAAGGATATAAGCAATTTAAAGCTGAGGTAGACCTTCTTCTCAGAGTACACCACATAAATTTAGTAACTCTTGTTGGTTATTGTGACGAAGGACAACACTTGGTCCTCATCTACGAGTACATGTCCAATGGAAACTTAAAACAACATCTATCTGGAGAAAATTCTAGGTCCCCTTTGAGTTGGGAAAATAGGCTAAGAATTGCTGCAGAGACAGCACAAGGACTGGAGTACTTGCATATCGGCTGCAAACCACCAATGATTCATAGAGATATCAAATCTATGAATATACTTCTTGATAATAATTTCCAGGCCAAACTTGGTGATTTCGGGCTTTCAAGATCTTTTCCGGTGGGAAGTGAAACTCATGTGTCAACAAATGTTGCTGGATCTCCAGGTTATCTTGACCCTGAATATTACCGAACAAACTGGTTGACAGAGAAGAGTGATGTCTTCAGTTTTGGAGTCGTATTACTGGAAATCATCACAAGTCAGCCAGTGATTGACCAAACTCGTGAGAAGTCTCACATAGGAGAATGGGTGGGATTTAAGTTAACCAATGGAGATATCAAAAATATTGTGGATCCAAGTATGAACGGTGATTATGACTCTAGTTCATTGTGGAAGGCTCTTGAGTTAGCAATGTCATGCGTCAGTCCATCTTCATCCGGAAGACCAAATATGTCCCAGGTTGCTAATGAACTACAAGAGTGTCTCTTAACTGAGAACTCAAGGAAAGGAGGGAGACATGACGTGGACTCCAAGAGTTCTCTTGAACAGAGCACAAGCTTTGGTCCTGAACATATCCCTGATGCACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001653 GO:0002229 GO:0002237 GO:0002238 GO:0002239 GO:0002376 GO:0002831 GO:0002832 GO:0002833 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009787 GO:0009788 GO:0009790 GO:0009791 GO:0009793 GO:0009845 GO:0009888 GO:0009960 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010154 GO:0010200 GO:0010243 GO:0010646 GO:0010648 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022414 GO:0023051 GO:0023052 GO:0023057 GO:0031347 GO:0031348 GO:0031349 GO:0032101 GO:0032102 GO:0032103 GO:0032501 GO:0032502 GO:0032879 GO:0036211 GO:0038023 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0042995 GO:0043170 GO:0043207 GO:0043412 GO:0043900 GO:0043901 GO:0043902 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0046777 GO:0046983 GO:0048316 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0090351 GO:0090406 GO:0098542 GO:0120025 GO:0140096 GO:1900150 GO:1900424 GO:1900426 GO:1901419 GO:1901420 GO:1901564 GO:1901698 GO:1901700 GO:1902288 GO:1902289 GO:1905957 GO:1905958 GO:2000071
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Malectin_like Pkinase Pkinase_Tyr

Protein Analysis

898

Amino Acids

100.69

Weight (kDa)

5.45

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 33 - 372 2.4e-117 Malectin-like domain
LRR_8 PF13855 451 - 490 4.7e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 593 - 746 1.4e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 594 - 763 2.1e-34 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0027168)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20450 AT4G20450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1161
Acc65I GGTACC 2 cut(s) 197, 467
AccB1I GGYRCC 3 cut(s) 197, 467, 1408
AccB7I CCANNNNNTGG 1 cut(s) 402
AccI GTMKAC 2 cut(s) 708, 1908
AccIII TCCGGA 1 cut(s) 2543
AciI CCGC 7 cut(s) 105, 186, 625, 716, 1032, 1473, 1760
AclI AACGTT 1 cut(s) 1523
AclWI GGATC 8 cut(s) 419, 895, 1214, 1550, 1563, 2272, 2453, 2466
AcsI RAATTY 6 cut(s) 154, 374, 415, 1415, 1936, 2038
AcuI CTGAAG 3 cut(s) 291, 1118, 2314
AcvI CACGTG 1 cut(s) 880
AcyI GRCGYC 2 cut(s) 542, 1158
AfiI CCNNNNNNNGG 5 cut(s) 39, 402, 434, 700, 1552
AflIII ACRYGT 3 cut(s) 879, 1269, 1998
AgeI ACCGGT 1 cut(s) 146
AgsI TTSAA 8 cut(s) 154, 659, 801, 913, 1171, 1181, 2214, 2651
AjiI CACGTC 1 cut(s) 2629
AjnI CCWGG 5 cut(s) 845, 1582, 2184, 2269, 2562
AjuI GAANNNNNNNTTGG 4 cut(s) 1282, 1314, 1788, 1820
Alw21I GWGCWC 4 cut(s) 280, 842, 1863, 2660
Alw26I GTCTC 5 cut(s) 1777, 2081, 2403, 2594, 2614
AlwI GGATC 8 cut(s) 419, 895, 1214, 1550, 1563, 2272, 2453, 2466
Ama87I CYCGRG 1 cut(s) 1021
Aor13HI TCCGGA 1 cut(s) 2543
AoxI GGCC 4 cut(s) 30, 391, 1252, 2187
ApeKI GCWGC 5 cut(s) 183, 253, 1628, 2081, 2121
ApoI RAATTY 6 cut(s) 154, 374, 415, 1415, 1936, 2038
AsiGI ACCGGT 1 cut(s) 146
Asp700I GAANNNNTTC 3 cut(s) 9, 644, 1297
Asp718I GGTACC 2 cut(s) 197, 467
AspS9I GGNCC 8 cut(s) 31, 392, 1332, 1378, 1580, 1981, 2046, 2669
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 3 cut(s) 1046, 2210, 2486
AvaI CYCGRG 1 cut(s) 1021
AvaII GGWCC 6 cut(s) 1332, 1378, 1580, 1981, 2046, 2669
BaeGI GKGCMC 1 cut(s) 314
BamHI GGATCC 2 cut(s) 1555, 2458
BanI GGYRCC 3 cut(s) 197, 467, 1408
BarI GAAGNNNNNNTAC 2 cut(s) 1899, 1931
BauI CACGAG 1 cut(s) 2388
BbrPI CACGTG 1 cut(s) 880
BbsI GAAGAC 3 cut(s) 219, 2320, 2554
Bbv12I GWGCWC 4 cut(s) 280, 842, 1863, 2660
BbvCI CCTCAGC 2 cut(s) 1873, 1902
BbvI GCAGC 5 cut(s) 195, 265, 1615, 2068, 2108
BccI CCATC 8 cut(s) 209, 458, 671, 1034, 1099, 1567, 1705, 2542
BceAI ACGGC 1 cut(s) 317
BciT130I CCWGG 5 cut(s) 847, 1584, 2186, 2271, 2564
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 5 cut(s) 1777, 2081, 2403, 2594, 2614
BfaI CTAG 7 cut(s) 126, 986, 1055, 1607, 2043, 2487, 2695
BfmI CTRYAG 4 cut(s) 976, 1261, 1367, 2082
BglII AGATCT 2 cut(s) 1057, 2216
BisI GCNGC 6 cut(s) 184, 254, 1474, 1629, 2082, 2122
BlsI GCNGC 6 cut(s) 185, 255, 1475, 1630, 2083, 2123
BmcAI AGTACT 1 cut(s) 2108
Bme1390I CCNGG 6 cut(s) 847, 863, 1584, 2186, 2271, 2564
Bme18I GGWCC 6 cut(s) 1332, 1378, 1580, 1981, 2046, 2669
BmeT110I CYCGRG 1 cut(s) 1021
BmgBI CACGTC 1 cut(s) 2629
BmgT120I GGNCC 8 cut(s) 31, 392, 1332, 1378, 1580, 1981, 2046, 2669
BmiI GGNNCC 8 cut(s) 199, 394, 469, 1333, 1410, 1557, 2048, 2460
BmrFI CCNGG 6 cut(s) 847, 863, 1584, 2186, 2271, 2564
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 3 cut(s) 58, 1631, 2677
BmuI ACTGGG 1 cut(s) 912
BpiI GAAGAC 3 cut(s) 219, 2320, 2554
BpmI CTGGAG 4 cut(s) 1109, 2052, 2123, 2253
Bpu10I CCTNAGC 3 cut(s) 257, 1873, 1902
BpuEI CTTGAG 6 cut(s) 57, 595, 1474, 1863, 2528, 2590
BpuMI CCSGG 1 cut(s) 863
BsaAI YACGTR 1 cut(s) 880
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 2 cut(s) 542, 1158
BsaI GGTCTC 1 cut(s) 1777
BsaJI CCNNGG 4 cut(s) 558, 1583, 1819, 2562
BsaWI WCCGGW 3 cut(s) 146, 2224, 2543
BsaXI ACNNNNNCTCC 4 cut(s) 2400, 2430, 2618, 2648
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 402, 434, 700, 1552
Bse118I RCCGGY 2 cut(s) 146, 602
Bse1I ACTGG 8 cut(s) 918, 1214, 1255, 1582, 2106, 2309, 2355, 2372
Bse3DI GCAATG 1 cut(s) 2523
Bse8I GATNNNNATC 1 cut(s) 384
BseAI TCCGGA 1 cut(s) 2543
BseBI CCWGG 5 cut(s) 847, 1584, 2186, 2271, 2564
BseDI CCNNGG 4 cut(s) 558, 1583, 1819, 2562
BseGI GGATG 6 cut(s) 161, 554, 673, 724, 1697, 2540
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 5 cut(s) 39, 402, 434, 700, 1552
BseMI GCAATG 1 cut(s) 2523
BseMII CTCAG 4 cut(s) 1887, 1893, 1933, 2589
BseNI ACTGG 8 cut(s) 918, 1214, 1255, 1582, 2106, 2309, 2355, 2372
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 5 cut(s) 195, 265, 1615, 2068, 2108
BshFI GGCC 4 cut(s) 32, 393, 1254, 2189
BshNI GGYRCC 3 cut(s) 197, 467, 1408
BshTI ACCGGT 1 cut(s) 146
BsiHKAI GWGCWC 4 cut(s) 280, 842, 1863, 2660
BsiHKCI CYCGRG 1 cut(s) 1021
BsiSI CCGG 5 cut(s) 147, 603, 863, 2225, 2544
BslFI GGGAC 5 cut(s) 555, 716, 1345, 2032, 2545
BslI CCNNNNNNNGG 5 cut(s) 39, 402, 434, 700, 1552
BsmAI GTCTC 5 cut(s) 1777, 2081, 2403, 2594, 2614
BsmFI GGGAC 5 cut(s) 555, 716, 1345, 2032, 2545
BsmI GAATGC 1 cut(s) 114
BsnI GGCC 4 cut(s) 32, 393, 1254, 2189
Bso31I GGTCTC 1 cut(s) 1777
BsoBI CYCGRG 1 cut(s) 1021
Bsp1286I GDGCHC 5 cut(s) 280, 314, 842, 1863, 2660
Bsp13I TCCGGA 1 cut(s) 2543
BspACI CCGC 7 cut(s) 105, 186, 625, 716, 1032, 1473, 1760
BspANI GGCC 4 cut(s) 32, 393, 1254, 2189
BspCNI CTCAG 4 cut(s) 1886, 1894, 1932, 2590
BspEI TCCGGA 1 cut(s) 2543
BspLI GGNNCC 8 cut(s) 199, 394, 469, 1333, 1410, 1557, 2048, 2460
BspMAI CTGCAG 1 cut(s) 2086
BspPI GGATC 8 cut(s) 419, 895, 1214, 1550, 1563, 2272, 2453, 2466
BspT107I GGYRCC 3 cut(s) 197, 467, 1408
BspTNI GGTCTC 1 cut(s) 1777
BsrDI GCAATG 1 cut(s) 2523
BsrFI RCCGGY 2 cut(s) 146, 602
BsrI ACTGG 8 cut(s) 918, 1214, 1255, 1582, 2106, 2309, 2355, 2372
BssAI RCCGGY 2 cut(s) 146, 602
BssECI CCNNGG 4 cut(s) 558, 1583, 1819, 2562
BssNAI GTATAC 1 cut(s) 709
BssNI GRCGYC 2 cut(s) 542, 1158
BssSI CACGAG 1 cut(s) 2388
BssT1I CCWWGG 1 cut(s) 558
Bst1107I GTATAC 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 2388
Bst2UI CCWGG 5 cut(s) 847, 1584, 2186, 2271, 2564
Bst6I CTCTTC 3 cut(s) 1089, 1746, 2312
BstACI GRCGYC 2 cut(s) 542, 1158
BstAPI GCANNNNNTGC 1 cut(s) 1628
BstBAI YACGTR 1 cut(s) 880
BstC8I GCNNGC 1 cut(s) 2692
BstDSI CCRYGG 1 cut(s) 1819
BstF5I GGATG 6 cut(s) 161, 554, 673, 724, 1697, 2540
BstMAI GTCTC 5 cut(s) 1777, 2081, 2403, 2594, 2614
BstMWI GCNNNNNNNGC 4 cut(s) 102, 250, 1628, 2090
BstNI CCWGG 5 cut(s) 847, 1584, 2186, 2271, 2564
BstNSI RCATGY 3 cut(s) 493, 1273, 2002
BstSCI CCNGG 6 cut(s) 845, 861, 1582, 2184, 2269, 2562
BstSFI CTRYAG 4 cut(s) 976, 1261, 1367, 2082
BstSLI GKGCMC 1 cut(s) 314
BstV1I GCAGC 5 cut(s) 195, 265, 1615, 2068, 2108
BstV2I GAAGAC 3 cut(s) 219, 2320, 2554
BstX2I RGATCY 8 cut(s) 424, 900, 1057, 1219, 1555, 2216, 2264, 2458
BstYI RGATCY 8 cut(s) 424, 900, 1057, 1219, 1555, 2216, 2264, 2458
BstZ17I GTATAC 1 cut(s) 709
BsuRI GGCC 4 cut(s) 32, 393, 1254, 2189
BtgI CCRYGG 1 cut(s) 1819
BtgZI GCGATG 1 cut(s) 1118
BtrI CACGTC 1 cut(s) 2629
BtsCI GGATG 6 cut(s) 161, 554, 673, 724, 1697, 2540
BtsIMutI CAGTG 3 cut(s) 925, 1002, 2379
Cac8I GCNNGC 1 cut(s) 2692
Cfr10I RCCGGY 2 cut(s) 146, 602
Cfr13I GGNCC 8 cut(s) 31, 392, 1332, 1378, 1580, 1981, 2046, 2669
CseI GACGC 3 cut(s) 176, 550, 2515
CsiI ACCWGGT 1 cut(s) 845
CspAI ACCGGT 1 cut(s) 146
DraI TTTAAA 1 cut(s) 1897
Eam1104I CTCTTC 3 cut(s) 1089, 1746, 2312
EarI CTCTTC 3 cut(s) 1089, 1746, 2312
Eco130I CCWWGG 1 cut(s) 558
Eco31I GGTCTC 1 cut(s) 1777
Eco32I GATATC 2 cut(s) 2149, 2443
Eco47I GGWCC 6 cut(s) 1332, 1378, 1580, 1981, 2046, 2669
Eco57I CTGAAG 3 cut(s) 291, 1118, 2314
Eco72I CACGTG 1 cut(s) 880
Eco88I CYCGRG 1 cut(s) 1021
EcoO109I RGGNCCY 1 cut(s) 2046
EcoRII CCWGG 5 cut(s) 845, 1582, 2184, 2269, 2562
EcoRV GATATC 2 cut(s) 2149, 2443
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FalI AAGNNNNNCTT 4 cut(s) 1282, 1314, 1729, 1761
FaqI GGGAC 5 cut(s) 555, 716, 1345, 2032, 2545
FauI CCCGC 1 cut(s) 632
FblI GTMKAC 2 cut(s) 708, 1908
Fnu4HI GCNGC 6 cut(s) 184, 254, 1474, 1629, 2082, 2122
FokI GGATG 6 cut(s) 148, 541, 660, 731, 1684, 2527
Fsp4HI GCNGC 6 cut(s) 184, 254, 1474, 1629, 2082, 2122
FspBI CTAG 7 cut(s) 126, 986, 1055, 1607, 2043, 2487, 2695
GluI GCNGC 6 cut(s) 184, 254, 1474, 1629, 2082, 2122
GsuI CTGGAG 4 cut(s) 1109, 2052, 2123, 2253
HaeIII GGCC 4 cut(s) 32, 393, 1254, 2189
HapII CCGG 5 cut(s) 147, 603, 863, 2225, 2544
HgaI GACGC 3 cut(s) 176, 550, 2515
Hin1I GRCGYC 2 cut(s) 542, 1158
HincII GTYRAC 6 cut(s) 1080, 1440, 1669, 2250, 2310, 2430
HindII GTYRAC 6 cut(s) 1080, 1440, 1669, 2250, 2310, 2430
HindIII AAGCTT 3 cut(s) 17, 1704, 2662
HpaI GTTAAC 1 cut(s) 2430
HpaII CCGG 5 cut(s) 147, 603, 863, 2225, 2544
HphI GGTGA 3 cut(s) 1046, 2210, 2486
Hpy188I TCNGA 8 cut(s) 166, 220, 297, 329, 450, 1137, 1867, 1922
Hpy99I CGWCG 6 cut(s) 140, 170, 544, 872, 875, 1160
HpyAV CCTTC 8 cut(s) 266, 438, 896, 1502, 1790, 1922, 1961, 2494
HpyCH4IV ACGT 7 cut(s) 568, 705, 873, 879, 1158, 1523, 2628
HpyCH4V TGCA 9 cut(s) 307, 421, 460, 824, 1622, 2084, 2113, 2124, 2690
HpyF10VI GCNNNNNNNGC 4 cut(s) 102, 250, 1628, 2090
HpySE526I ACGT 7 cut(s) 568, 705, 873, 879, 1158, 1523, 2628
Hsp92I GRCGYC 2 cut(s) 542, 1158
Kpn2I TCCGGA 1 cut(s) 2543
KpnI GGTACC 2 cut(s) 201, 471
KspAI GTTAAC 1 cut(s) 2430
LmnI GCTCC 3 cut(s) 275, 837, 1055
Lsp1109I GCAGC 5 cut(s) 195, 265, 1615, 2068, 2108
LweI GCATC 3 cut(s) 58, 1631, 2677
MabI ACCWGGT 1 cut(s) 845
MaeI CTAG 7 cut(s) 126, 986, 1055, 1607, 2043, 2487, 2695
MaeII ACGT 7 cut(s) 568, 705, 873, 879, 1158, 1523, 2628
MaeIII GTNAC 6 cut(s) 116, 1034, 1119, 1159, 1942, 1961
MflI RGATCY 8 cut(s) 424, 900, 1057, 1219, 1555, 2216, 2264, 2458
MhlI GDGCHC 5 cut(s) 280, 314, 842, 1863, 2660
MlyI GAGTC 7 cut(s) 131, 149, 209, 1818, 2349, 2477, 2627
MmeI TCCRAC 1 cut(s) 189
MroI TCCGGA 1 cut(s) 2543
MroXI GAANNNNTTC 3 cut(s) 9, 644, 1297
MseI TTAA 9 cut(s) 525, 768, 1325, 1359, 1896, 2015, 2424, 2429, 2594
MslI CAYNNNNRTG 3 cut(s) 678, 717, 1824
MspA1I CMGCKG 3 cut(s) 186, 627, 1628
MspI CCGG 5 cut(s) 147, 603, 863, 2225, 2544
MspR9I CCNGG 6 cut(s) 847, 863, 1584, 2186, 2271, 2564
Mva1269I GAATGC 1 cut(s) 114
MvaI CCWGG 5 cut(s) 847, 1584, 2186, 2271, 2564
MwoI GCNNNNNNNGC 4 cut(s) 102, 250, 1628, 2090
NciI CCSGG 1 cut(s) 863
NlaIV GGNNCC 8 cut(s) 199, 394, 469, 1333, 1410, 1557, 2048, 2460
NmeAIII GCCGAG 1 cut(s) 922
NmuCI GTSAC 3 cut(s) 1034, 1159, 1961
NspI RCATGY 3 cut(s) 493, 1273, 2002
PaeR7I CTCGAG 1 cut(s) 1021
PciI ACATGT 2 cut(s) 1269, 1998
PcsI WCGNNNNNNNCGW 1 cut(s) 876
PctI GAATGC 1 cut(s) 114
PdmI GAANNNNTTC 3 cut(s) 9, 644, 1297
PfeI GAWTC 6 cut(s) 87, 221, 659, 1293, 1868, 2137
PflMI CCANNNNNTGG 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 146
PkrI GCNGC 6 cut(s) 185, 255, 1475, 1630, 2083, 2123
PleI GAGTC 7 cut(s) 130, 148, 209, 1817, 2348, 2477, 2627
PmaCI CACGTG 1 cut(s) 880
PmlI CACGTG 1 cut(s) 880
PpsI GAGTC 7 cut(s) 130, 148, 209, 1817, 2348, 2477, 2627
Ppu21I YACGTR 1 cut(s) 880
PpuMI RGGWCCY 1 cut(s) 2046
PscI ACATGT 2 cut(s) 1269, 1998
Psp1406I AACGTT 1 cut(s) 1523
Psp5II RGGWCCY 1 cut(s) 2046
Psp6I CCWGG 5 cut(s) 845, 1582, 2184, 2269, 2562
PspCI CACGTG 1 cut(s) 880
PspGI CCWGG 5 cut(s) 845, 1582, 2184, 2269, 2562
PspN4I GGNNCC 8 cut(s) 199, 394, 469, 1333, 1410, 1557, 2048, 2460
PspPI GGNCC 8 cut(s) 31, 392, 1332, 1378, 1580, 1981, 2046, 2669
PspPPI RGGWCCY 1 cut(s) 2046
PspXI VCTCGAGB 1 cut(s) 1021
PstI CTGCAG 1 cut(s) 2086
PsuI RGATCY 8 cut(s) 424, 900, 1057, 1219, 1555, 2216, 2264, 2458
PvuII CAGCTG 1 cut(s) 1628
RseI CAYNNNNRTG 3 cut(s) 678, 717, 1824
SaqAI TTAA 9 cut(s) 525, 768, 1325, 1359, 1896, 2015, 2424, 2429, 2594
SatI GCNGC 6 cut(s) 184, 254, 1474, 1629, 2082, 2122
Sau96I GGNCC 8 cut(s) 31, 392, 1332, 1378, 1580, 1981, 2046, 2669
ScaI AGTACT 1 cut(s) 2108
SchI GAGTC 7 cut(s) 131, 149, 209, 1818, 2349, 2477, 2627
ScrFI CCNGG 6 cut(s) 847, 863, 1584, 2186, 2271, 2564
SduI GDGCHC 5 cut(s) 280, 314, 842, 1863, 2660
SexAI ACCWGGT 1 cut(s) 845
SfaNI GCATC 3 cut(s) 58, 1631, 2677
SfcI CTRYAG 4 cut(s) 976, 1261, 1367, 2082
Sfr274I CTCGAG 1 cut(s) 1021
SinI GGWCC 6 cut(s) 1332, 1378, 1580, 1981, 2046, 2669
SlaI CTCGAG 1 cut(s) 1021
SmiMI CAYNNNNRTG 3 cut(s) 678, 717, 1824
SmlI CTYRAG 7 cut(s) 72, 574, 1021, 1453, 1878, 2507, 2605
SmoI CTYRAG 7 cut(s) 72, 574, 1021, 1453, 1878, 2507, 2605
SsiI CCGC 7 cut(s) 105, 186, 625, 716, 1032, 1473, 1760
SspI AATATT 2 cut(s) 2291, 2452
SspMI CTAG 7 cut(s) 126, 986, 1055, 1607, 2043, 2487, 2695
StyD4I CCNGG 6 cut(s) 845, 861, 1582, 2184, 2269, 2562
StyI CCWWGG 1 cut(s) 558
TaiI ACGT 7 cut(s) 571, 708, 876, 882, 1161, 1526, 2631
TaqI TCGA 2 cut(s) 851, 1022
TaqII GACCGA 2 cut(s) 224, 1154
TatI WGTACW 3 cut(s) 1924, 1995, 2106
TauI GCSGC 1 cut(s) 1476
TfiI GAWTC 6 cut(s) 87, 221, 659, 1293, 1868, 2137
Tru1I TTAA 9 cut(s) 525, 768, 1325, 1359, 1896, 2015, 2424, 2429, 2594
Tru9I TTAA 9 cut(s) 525, 768, 1325, 1359, 1896, 2015, 2424, 2429, 2594
TscAI CASTG 3 cut(s) 925, 1009, 2379
TseFI GTSAC 3 cut(s) 1034, 1159, 1961
TseI GCWGC 5 cut(s) 183, 253, 1628, 2081, 2121
Tsp45I GTSAC 3 cut(s) 1034, 1159, 1961
TspGWI ACGGA 1 cut(s) 1266
TspRI CASTG 3 cut(s) 925, 1009, 2379
Van91I CCANNNNNTGG 1 cut(s) 402
VpaK11BI GGWCC 6 cut(s) 1332, 1378, 1580, 1981, 2046, 2669
XapI RAATTY 6 cut(s) 154, 374, 415, 1415, 1936, 2038
XbaI TCTAGA 1 cut(s) 985
XceI RCATGY 3 cut(s) 493, 1273, 2002
XcmI CCANNNNNNNNNTGG 1 cut(s) 2192
XhoI CTCGAG 1 cut(s) 1021
XmiI GTMKAC 2 cut(s) 708, 1908
XmnI GAANNNNTTC 3 cut(s) 9, 644, 1297
XspI CTAG 7 cut(s) 126, 986, 1055, 1607, 2043, 2487, 2695
ZraI GACGTC 1 cut(s) 1159
ZrmI AGTACT 1 cut(s) 2108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.