AT4G22940

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0027458
Physical Location & Seq
Reverse (-)
12021344 .. 12023563
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22940.2

Sequence Viewer

Length: 1377 bp
ATGGGTTGTATCATTTCTTATCGGAGGAATAAACCGGCGGCACCAACAGGATTGCCGGAAAACCGTCCTCGTTTCCATACTCTTCCATCAGAAAGAAGAATAGCAAATGATCAAGCCCAAAAGGTGACAGAATTGTTGAAGATGAGTGATAAATTAGGTCCAAAGGAGCTGAAGAGAGAAGAAAGTATTCTCGTTGTTAATGTTCATCCTCGCTCATCGGAATTAGCTGCCTCTGGTTGGCCACCGTGGCTTATTTCTGTAGCTGGTGAAGCCCTTGTTGGTTGGACCCCTGGTCGTGAATCTCACTTTGAGAAGCAAGAACAAATTGGGGGAGGAACATTCAGTAAGGTGTTCAAAGCTAGAGATCTTCTTCGTAACAAGACCGTGGCTTTGAAGAGAATCCGGTTCGATATCAACAACTCAGAAAGCATCAAATGTATAGCCAGAGAGATCATAATCCTGCGCAAGTTGGATCATCCCAATGTCATCAAATTAGAAGGGTTAATGCTTGTAGACCACGATTCCTCTACCCTTTACCTGATCTTTGAGTACATGGAACATGACCTCTTGGGACTCTCTTCATTACTCGGTGTACATTTCTCAGAACCTCAGGTTAAATGCTACATGAGGCAGCTCTTAAGAGGGCTTGATCATTGTCACACTAATCATGTACTCCATCGGGATATGAAAAGCTCGAACCTTCTGATCAACGGTGATGGAGTCCTCAAGATTGCAGATTTTGGGTTAGCTACGTTCTTCGATCCTCATAACAGTGTTCCTTTGACAACCCATGTTGCAACTCTATGGTATCGACCTCCTGAGCTTTTGCTTGGAGCTTCTCATTACGGTATTGGAGTTGACTTGTGGAGCACAGGCTGCGTAATAGGTGAATTATATGCCGGAAAGCCTATTCTTCCAGGAAAAAATGAGACAGACCAACTGCACAAGATATTCAAGTTGTGTGGATCGCCTTCAGATGATTATTGGACAAAACTAAAGCTACAGCTTTCAACTCCACTGAGACCCATCTATCCATATGGATCACATATAGCAGAGACATTTAAACAGTTTCCAGCTTCAGTTATTTCGCTCTTAGAAACTTTGCTCTCAATAGATCCAGATTTCCGAGGCACTGCAGCTAGTGCTCTCAAGAGTAAATACTTCAAAACTGAGCCATTGGCTTGTGATCCATCTTGTCTACCAAAATACCCTTCAAGTAAGGAAATCAACATCAAGATGCGCGATAACACTAGAAAACAAGCTTCACAAATAAGAAGAACAGACGAAGCTCAAGCTGTTCAGCCAATTCTAGCAGATTCGTCTCCCACAAAGCCGATGCAGGTATCTCTCTACTCTTACGGTTTCTTGGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

458

Amino Acids

51.41

Weight (kDa)

8.99

Isoelectric Point (pI)

46.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 103 - 388 5.4e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 104 - 317 5.9e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027458)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22940 AT4G22940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 464
Acc36I ACCTGC 1 cut(s) 1330
AccB1I GGYRCC 1 cut(s) 40
AccI GTMKAC 2 cut(s) 513, 1198
AccII CGCG 1 cut(s) 1242
AciI CCGC 1 cut(s) 38
AclWI GGATC 6 cut(s) 480, 755, 973, 1048, 1109, 1181
AcoI YGGCCR 1 cut(s) 239
AcuI CTGAAG 3 cut(s) 191, 957, 1062
AfaI GTAC 3 cut(s) 551, 594, 672
AfiI CCNNNNNNNGG 1 cut(s) 237
AflII CTTAAG 1 cut(s) 637
AgsI TTSAA 7 cut(s) 139, 355, 394, 955, 1011, 1165, 1215
AhdI GACNNNNNGTC 1 cut(s) 291
AjnI CCWGG 2 cut(s) 289, 916
AjuI GAANNNNNNNTTGG 4 cut(s) 261, 293, 1195, 1227
Alw21I GWGCWC 2 cut(s) 872, 1147
Alw26I GTCTC 4 cut(s) 923, 1015, 1049, 1326
AlwI GGATC 6 cut(s) 480, 755, 973, 1048, 1109, 1181
AoxI GGCC 1 cut(s) 239
ApeKI GCWGC 4 cut(s) 227, 631, 876, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 373, 405
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 2 cut(s) 465, 1242
AspS9I GGNCC 2 cut(s) 158, 285
AsuHPI GGTGA 4 cut(s) 136, 278, 725, 899
AvaII GGWCC 2 cut(s) 158, 285
AxyI CCTNAGG 1 cut(s) 609
BalI TGGCCA 1 cut(s) 241
BanI GGYRCC 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 872, 1147
BbvI GCAGC 4 cut(s) 214, 643, 863, 1148
BccI CCATC 5 cut(s) 94, 684, 710, 1034, 1198
BciT130I CCWGG 2 cut(s) 291, 918
BclI TGATCA 3 cut(s) 109, 649, 705
BcoDI GTCTC 4 cut(s) 923, 1015, 1049, 1326
BfaI CTAG 4 cut(s) 360, 1140, 1251, 1310
BfmI CTRYAG 3 cut(s) 258, 1001, 1134
BfrI CTTAAG 1 cut(s) 637
BfuAI ACCTGC 1 cut(s) 1330
BglI GCCNNNNNGGC 1 cut(s) 247
BglII AGATCT 1 cut(s) 364
BisI GCNGC 5 cut(s) 39, 228, 632, 877, 1137
BlsI GCNGC 5 cut(s) 40, 229, 633, 878, 1138
Bme1390I CCNGG 2 cut(s) 291, 918
Bme18I GGWCC 2 cut(s) 158, 285
BmeRI GACNNNNNGTC 1 cut(s) 291
BmgT120I GGNCC 2 cut(s) 158, 285
BmiI GGNNCC 2 cut(s) 42, 287
BmrFI CCNGG 2 cut(s) 291, 918
BmsI GCATC 3 cut(s) 438, 1227, 1326
Bpu10I CCTNAGC 1 cut(s) 819
BpuEI CTTGAG 3 cut(s) 710, 1133, 1275
BsaBI GATNNNNATC 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 1015
BsaJI CCNNGG 4 cut(s) 245, 289, 384, 1126
BsaWI WCCGGW 1 cut(s) 402
Bsc4I CCNNNNNNNGG 1 cut(s) 237
Bse118I RCCGGY 1 cut(s) 34
Bse21I CCTNAGG 1 cut(s) 609
Bse8I GATNNNNATC 1 cut(s) 455
BseBI CCWGG 2 cut(s) 291, 918
BseDI CCNNGG 4 cut(s) 245, 289, 384, 1126
BseGI GGATG 2 cut(s) 205, 475
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 1 cut(s) 237
BseMII CTCAG 6 cut(s) 435, 615, 623, 810, 1010, 1161
BseXI GCAGC 4 cut(s) 214, 643, 863, 1148
BsgI GTGCAG 1 cut(s) 926
Bsh1236I CGCG 1 cut(s) 1242
BshFI GGCC 1 cut(s) 241
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 2 cut(s) 872, 1147
BsiSI CCGG 4 cut(s) 35, 56, 403, 900
BslFI GGGAC 1 cut(s) 585
BslI CCNNNNNNNGG 1 cut(s) 237
BsmAI GTCTC 4 cut(s) 923, 1015, 1049, 1326
BsmBI CGTCTC 1 cut(s) 1326
BsmFI GGGAC 1 cut(s) 585
BsnI GGCC 1 cut(s) 241
Bso31I GGTCTC 1 cut(s) 1015
Bsp1286I GDGCHC 2 cut(s) 872, 1147
Bsp1407I TGTACA 1 cut(s) 592
BspACI CCGC 1 cut(s) 38
BspANI GGCC 1 cut(s) 241
BspCNI CTCAG 6 cut(s) 434, 614, 622, 811, 1011, 1162
BspFNI CGCG 1 cut(s) 1242
BspLI GGNNCC 2 cut(s) 42, 287
BspMAI CTGCAG 1 cut(s) 1138
BspMI ACCTGC 1 cut(s) 1330
BspPI GGATC 6 cut(s) 480, 755, 973, 1048, 1109, 1181
BspT107I GGYRCC 1 cut(s) 40
BspTI CTTAAG 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 1015
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 592
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 4 cut(s) 245, 289, 384, 1126
Bst2UI CCWGG 2 cut(s) 291, 918
Bst4CI ACNGT 8 cut(s) 65, 246, 385, 713, 773, 848, 1068, 1361
Bst6I CTCTTC 4 cut(s) 87, 167, 389, 583
BstAFI CTTAAG 1 cut(s) 637
BstAPI GCANNNNNTGC 2 cut(s) 876, 1142
BstAUI TGTACA 1 cut(s) 592
BstDEI CTNAG 7 cut(s) 421, 601, 609, 819, 1019, 1093, 1170
BstDSI CCRYGG 2 cut(s) 245, 384
BstF5I GGATG 2 cut(s) 205, 475
BstFNI CGCG 1 cut(s) 1242
BstHHI GCGC 2 cut(s) 465, 1242
BstMAI GTCTC 4 cut(s) 923, 1015, 1049, 1326
BstMWI GCNNNNNNNGC 4 cut(s) 247, 269, 876, 1142
BstNI CCWGG 2 cut(s) 291, 918
BstSCI CCNGG 2 cut(s) 289, 916
BstSFI CTRYAG 3 cut(s) 258, 1001, 1134
BstUI CGCG 1 cut(s) 1242
BstV1I GCAGC 4 cut(s) 214, 643, 863, 1148
BstX2I RGATCY 2 cut(s) 364, 1114
BstYI RGATCY 2 cut(s) 364, 1114
Bsu36I CCTNAGG 1 cut(s) 609
BsuRI GGCC 1 cut(s) 241
BtgI CCRYGG 2 cut(s) 245, 384
BtsCI GGATG 2 cut(s) 205, 475
BtsI GCAGTG 1 cut(s) 1131
BtsIMutI CAGTG 3 cut(s) 778, 1016, 1131
BveI ACCTGC 1 cut(s) 1330
CfoI GCGC 2 cut(s) 465, 1242
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 158, 285
Csp6I GTAC 3 cut(s) 550, 593, 671
CspCI CAANNNNNGTGG 2 cut(s) 943, 978
CviAII CATG 5 cut(s) 553, 560, 625, 668, 791
CviQI GTAC 3 cut(s) 550, 593, 671
DdeI CTNAG 7 cut(s) 421, 601, 609, 819, 1019, 1093, 1170
DraI TTTAAA 1 cut(s) 1063
DriI GACNNNNNGTC 1 cut(s) 291
EaeI YGGCCR 1 cut(s) 239
Eam1104I CTCTTC 4 cut(s) 87, 167, 389, 583
Eam1105I GACNNNNNGTC 1 cut(s) 291
EarI CTCTTC 4 cut(s) 87, 167, 389, 583
Eco31I GGTCTC 1 cut(s) 1015
Eco32I GATATC 1 cut(s) 412
Eco47I GGWCC 2 cut(s) 158, 285
Eco57I CTGAAG 3 cut(s) 191, 957, 1062
Eco81I CCTNAGG 1 cut(s) 609
EcoRII CCWGG 2 cut(s) 289, 916
EcoRV GATATC 1 cut(s) 412
Esp3I CGTCTC 1 cut(s) 1326
FaeI CATG 5 cut(s) 556, 563, 628, 671, 794
FaqI GGGAC 1 cut(s) 585
FatI CATG 5 cut(s) 552, 559, 624, 667, 790
FauNDI CATATG 1 cut(s) 1036
FbaI TGATCA 3 cut(s) 109, 649, 705
FblI GTMKAC 2 cut(s) 513, 1198
Fnu4HI GCNGC 5 cut(s) 39, 228, 632, 877, 1137
FokI GGATG 2 cut(s) 192, 462
Fsp4HI GCNGC 5 cut(s) 39, 228, 632, 877, 1137
FspBI CTAG 4 cut(s) 360, 1140, 1251, 1310
FspI TGCGCA 1 cut(s) 464
GlaI GCGC 2 cut(s) 464, 1241
GluI GCNGC 5 cut(s) 39, 228, 632, 877, 1137
HaeIII GGCC 1 cut(s) 241
HapII CCGG 4 cut(s) 35, 56, 403, 900
HhaI GCGC 2 cut(s) 465, 1242
Hin1II CATG 5 cut(s) 556, 563, 628, 671, 794
Hin6I GCGC 2 cut(s) 463, 1240
HinP1I GCGC 2 cut(s) 463, 1240
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 1 cut(s) 1260
HinfI GANTC 6 cut(s) 299, 399, 521, 573, 720, 1316
HpaII CCGG 4 cut(s) 35, 56, 403, 900
HphI GGTGA 4 cut(s) 136, 278, 725, 899
Hpy166II GTNNAC 4 cut(s) 514, 593, 859, 1199
Hpy188I TCNGA 8 cut(s) 24, 91, 220, 424, 604, 705, 976, 1127
Hpy188III TCNNGA 8 cut(s) 296, 680, 727, 818, 1118, 1150, 1234, 1374
Hpy8I GTNNAC 4 cut(s) 514, 593, 859, 1199
HpyAV CCTTC 4 cut(s) 491, 710, 981, 1221
HpyCH4III ACNGT 8 cut(s) 65, 246, 385, 713, 773, 848, 1068, 1361
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 5 cut(s) 734, 797, 943, 1136, 1339
HpyF10VI GCNNNNNNNGC 4 cut(s) 247, 269, 876, 1142
HpyF3I CTNAG 7 cut(s) 421, 601, 609, 819, 1019, 1093, 1170
HpySE526I ACGT 1 cut(s) 752
Hsp92II CATG 5 cut(s) 556, 563, 628, 671, 794
HspAI GCGC 2 cut(s) 463, 1240
Ksp22I TGATCA 3 cut(s) 109, 649, 705
LmnI GCTCC 3 cut(s) 166, 833, 867
Lsp1109I GCAGC 4 cut(s) 214, 643, 863, 1148
LweI GCATC 3 cut(s) 438, 1227, 1326
MaeI CTAG 4 cut(s) 360, 1140, 1251, 1310
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 3 cut(s) 124, 374, 656
MflI RGATCY 2 cut(s) 364, 1114
MhlI GDGCHC 2 cut(s) 872, 1147
MlsI TGGCCA 1 cut(s) 241
MluCI AATT 7 cut(s) 131, 152, 221, 324, 491, 890, 1305
MluNI TGGCCA 1 cut(s) 241
MlyI GAGTC 2 cut(s) 567, 729
MmeI TCCRAC 2 cut(s) 263, 450
Mox20I TGGCCA 1 cut(s) 241
MroXI GAANNNNTTC 1 cut(s) 186
MscI TGGCCA 1 cut(s) 241
MseI TTAA 5 cut(s) 198, 503, 615, 638, 1062
MslI CAYNNNNRTG 3 cut(s) 480, 771, 1235
Msp20I TGGCCA 1 cut(s) 241
MspCI CTTAAG 1 cut(s) 637
MspI CCGG 4 cut(s) 35, 56, 403, 900
MspR9I CCNGG 2 cut(s) 291, 918
MvaI CCWGG 2 cut(s) 291, 918
MvnI CGCG 1 cut(s) 1242
MwoI GCNNNNNNNGC 4 cut(s) 247, 269, 876, 1142
NdeI CATATG 1 cut(s) 1036
NlaIII CATG 5 cut(s) 556, 563, 628, 671, 794
NlaIV GGNNCC 2 cut(s) 42, 287
NmuCI GTSAC 2 cut(s) 124, 656
NsbI TGCGCA 1 cut(s) 464
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 4 cut(s) 299, 399, 521, 1316
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 5 cut(s) 40, 229, 633, 878, 1138
PleI GAGTC 2 cut(s) 567, 728
PpsI GAGTC 2 cut(s) 567, 728
Psp6I CCWGG 2 cut(s) 289, 916
PspGI CCWGG 2 cut(s) 289, 916
PspN4I GGNNCC 2 cut(s) 42, 287
PspPI GGNCC 2 cut(s) 158, 285
PstI CTGCAG 1 cut(s) 1138
PsuI RGATCY 2 cut(s) 364, 1114
RsaI GTAC 3 cut(s) 551, 594, 672
RsaNI GTAC 3 cut(s) 550, 593, 671
RseI CAYNNNNRTG 3 cut(s) 480, 771, 1235
SaqAI TTAA 5 cut(s) 198, 503, 615, 638, 1062
SatI GCNGC 5 cut(s) 39, 228, 632, 877, 1137
Sau96I GGNCC 2 cut(s) 158, 285
SchI GAGTC 2 cut(s) 567, 729
ScrFI CCNGG 2 cut(s) 291, 918
SduI GDGCHC 2 cut(s) 872, 1147
SfaNI GCATC 3 cut(s) 438, 1227, 1326
SfcI CTRYAG 3 cut(s) 258, 1001, 1134
SinI GGWCC 2 cut(s) 158, 285
SmiMI CAYNNNNRTG 3 cut(s) 480, 771, 1235
SmlI CTYRAG 4 cut(s) 637, 725, 1148, 1290
SmoI CTYRAG 4 cut(s) 637, 725, 1148, 1290
Sse9I AATT 7 cut(s) 131, 152, 221, 324, 491, 890, 1305
SsiI CCGC 1 cut(s) 38
SspMI CTAG 4 cut(s) 360, 1140, 1251, 1310
StyD4I CCNGG 2 cut(s) 289, 916
TaaI ACNGT 8 cut(s) 65, 246, 385, 713, 773, 848, 1068, 1361
TaiI ACGT 1 cut(s) 755
TaqI TCGA 4 cut(s) 408, 695, 759, 811
TasI AATT 7 cut(s) 131, 152, 221, 324, 491, 890, 1305
TatI WGTACW 3 cut(s) 549, 592, 670
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 4 cut(s) 299, 399, 521, 1316
Tru1I TTAA 5 cut(s) 198, 503, 615, 638, 1062
Tru9I TTAA 5 cut(s) 198, 503, 615, 638, 1062
TscAI CASTG 3 cut(s) 778, 1023, 1138
TseFI GTSAC 2 cut(s) 124, 656
TseI GCWGC 4 cut(s) 227, 631, 876, 1136
Tsp45I GTSAC 2 cut(s) 124, 656
TspDTI ATGAA 3 cut(s) 194, 570, 701
TspRI CASTG 3 cut(s) 778, 1023, 1138
Vha464I CTTAAG 1 cut(s) 637
VpaK11BI GGWCC 2 cut(s) 158, 285
XmiI GTMKAC 2 cut(s) 513, 1198
XmnI GAANNNNTTC 1 cut(s) 186
XspI CTAG 4 cut(s) 360, 1140, 1251, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.