Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0020961

Physical Location & Seq

Genomic Coordinates Reverse (-)

12162004 .. 12167026

Gene Structure 5023 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G23250.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGGCAAAGACGAGTTGTGAAATAATCCTCTGTTTCTTCTTCTTCATTTTAAGCTTCAGGGTCATTTCTGTTTCTGCACAACAAACTTGTGACAACACTGCTGGTAGTTTCAAACCCAATAGTACTTATGACAACAACCGTCGTCTCCTCCTCTCTACCTTCGCCTCTAACGTCACGGCTCAGAACGGCTACTTCAATGGTTCGTTCGGGCTTGGTACAGACAGAGTCTACGCCATGGGGATGTGCGCTCCAGGTGCTGAACCTGATGTTTGCTCCAACTGTATTAAGAACACAGCAGAGGGTTTACTACAGATCTGTCTAAACCAGACGGACGGTTTCTCATGGTCTGGTGAAGAAACCCTTTGCCTTGTTCGTTACTCTAACAAATCCTTCTCCGGGCTACTTGGCTTGGAGCCGTCTAATGATTTCTTCAATGTAAACGAAATAAGAAAAGAAGACCAGAAGGAATTTGATAGTGTGTTTGATGAGTTAATGTTTCGTACAATCCAAGGAGCATCTTCATCGGTGAGAAACAATTCTAATTCGTTATCATTGTCTGGTAAATATTATGCAAAGGATGTAGCTCCTGAACCGGTTTACGGGAACATATCGGTGGTGATGCAATGCACTCCAGATGTTTCTTCAAAGGATTGTAATCTTTGTCTTGAGAGAAGTCTTGATTTCTATAAGAAATGGTACAATGGGAAGAGAGGCACCATTATATTGCGGCCAAGTTGCTTTTTCCGGTGGGAATTGTATACTTTCTTTGGTGCTTTTGATAGTATTAATGCTAGACACCCACCGCCTCCTCCACGCCCGCTTTCACCGCCCCCTTTAAAGACGCCTTCTGTAACCAACCAAACCAATATTACCAAGAAGAATGATAGTAGAATCTCAGGAGGAACTATCGCGGCGATAGTCGTTGTAGTTGTTGTTACAATAATATTGATTGTTGTAGGCTTGGTTATATGCAAGAGAAGAAAGCAAAAGCAAGAAATTGAACTTCCAATCGTTTTAAATGAAGCGGAATCTGTTCAGTTTGATTTAAAGACAATTGAAGCAGCAACGGGCAACTTTTCTGAGCATAACAAGCTTGGTGCAGGTGGATTTGGTGAAGTTTACAAGGGTATGCTTTTGAATGGGACTGAAATTGCGGTAAAAAGATTGTCAAAAACATCAGGACAAGGTGAAATAGAGTTCAAGAACGAGGTGGTTGTTGTTGCAAAGCTTCAACACATAAATCTTGTTAGACTTCTCGGGTTTTCGCTCCAAGGAGAAGAGAAGTTACTCGTCTATGAATTTGTTCCAAACAAAAGTCTCGATTATTTCCTTTTTGACCCTAACAAGAGAAATCAGTTGGACTGGACAGTGAGACGCAACATCATCGGTGGGATTACTCGAGGAATTCTATATCTTCATCAAGATTCACGGCTCAAAATCATACATCGTGACCTCAAAGCGAGTAACATTCTCTTAGATGCGGATATGAACCCGAAAATTGCTGATTTCGGAATGGCAAGGATCTTTGGAGTGGACCAAACTGTAGCCAATACCGCAAGAGTAGTTGGAACCTTTGGTTACATGTCTCCTGAGTATGTGACACATGGCCAATTCTCAATGAAATCAGACGTCTATAGTTTTGGAGTATTGATTCTTGAGATCATTAGTGGCAAAAAGAATAGCAGCTTCTATCAGATGGATGGCTTAGTTAACAATTTAGTCACATATGTCTGGAAACTTTGGGAGAACAAAACAATGCATGAGCTCATAGATCCCTTTATTAAAGAAGATTGTAAAAGCGATGAAGTCATTAGATACGTTCATATTGGGCTGTTATGTGTTCAAGAAAATCCTGCAGATCGACCAACAATGTCTACGATTCACCAAGTGCTCACAACCAGCTCAATTACTCTACCTGTGCCTCAGCCACCTGGATTTTTCTTCAGGAACGGACCAGGATCGAACCCATCAAGCCAGGGAATGGTGCCTGGTCAATCGAGCAGCAAGTCTTTTACTAGTTCTGTAGATGAAGCAACAATCACCCAAGTTAATCCTCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0051704 GO:0051707 GO:0071704 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr Stress-antifung

Protein Analysis

686

Amino Acids

76.35

Weight (kDa)

8.05

Isoelectric Point (pI)

46.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Stress-antifung	PF01657	29 - 129	2.5e-19	Salt stress response/antifungal
Stress-antifung	PF01657	153 - 252	2.1e-07	Salt stress response/antifungal
Pkinase	PF00069	355 - 623	2.9e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	357 - 626	2.1e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0020961)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G23250 AT4G23250 AT4G23250 AT4G23260 AT4G23260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1091
AatII	GACGTC	1 cut(s)	1632
Acc36I	ACCTGC	1 cut(s)	1091
AccB1I	GGYRCC	2 cut(s)	713, 1984
AccB7I	CCANNNNNTGG	1 cut(s)	1981
AccI	GTMKAC	3 cut(s)	228, 758, 1874
AccII	CGCG	1 cut(s)	911
AciI	CCGC	9 cut(s)	727, 803, 818, 827, 911, 1025, 1154, 1481, 1554
AclWI	GGATC	3 cut(s)	1529, 1766, 1966
AcoI	YGGCCR	2 cut(s)	728, 1606
AcsI	RAATTY	3 cut(s)	467, 1298, 1404
AcuI	CTGAAG	2 cut(s)	40, 1927
AcyI	GRCGYC	2 cut(s)	842, 1629
AdeI	CACNNNGTG	1 cut(s)	1888
AfaI	GTAC	4 cut(s)	124, 217, 502, 698
AfiI	CCNNNNNNNGG	3 cut(s)	396, 599, 1981
AflIII	ACRYGT	1 cut(s)	1581
AgeI	ACCGGT	1 cut(s)	592
AhlI	ACTAGT	1 cut(s)	2015
AjnI	CCWGG	5 cut(s)	250, 1930, 1954, 1974, 1987
AluBI	AGCT	7 cut(s)	54, 584, 1093, 1228, 1686, 1765, 1902
AluI	AGCT	7 cut(s)	54, 584, 1093, 1228, 1686, 1765, 1902
Alw21I	GWGCWC	2 cut(s)	1767, 1893
Alw26I	GTCTC	4 cut(s)	149, 1322, 1366, 1590
AlwI	GGATC	3 cut(s)	1529, 1766, 1966
AlwNI	CAGNNNCTG	1 cut(s)	257
Ama87I	CYCGRG	2 cut(s)	1256, 1398
AoxI	GGCC	2 cut(s)	728, 1606
ApeKI	GCWGC	3 cut(s)	1061, 1683, 2001
ApoI	RAATTY	3 cut(s)	467, 1298, 1404
AseI	ATTAAT	1 cut(s)	786
AsiGI	ACCGGT	1 cut(s)	592
Asp700I	GAANNNNTTC	3 cut(s)	535, 1032, 1302
AspLEI	GCGC	1 cut(s)	248
AspS9I	GGNCC	2 cut(s)	1534, 1952
AsuC2I	CCSGG	1 cut(s)	397
AsuHPI	GGTGA	8 cut(s)	362, 538, 628, 816, 1124, 1199, 1874, 2032
AvaI	CYCGRG	2 cut(s)	1256, 1398
AvaII	GGWCC	2 cut(s)	1534, 1952
BalI	TGGCCA	1 cut(s)	1608
BanI	GGYRCC	2 cut(s)	713, 1984
BanII	GRGCYC	1 cut(s)	1767
BarI	GAAGNNNNNNTAC	2 cut(s)	1269, 1301
BbsI	GAAGAC	1 cut(s)	462
Bbv12I	GWGCWC	2 cut(s)	1767, 1893
BbvCI	CCTCAGC	1 cut(s)	1923
BbvI	GCAGC	3 cut(s)	1073, 1695, 2013
BccI	CCATC	3 cut(s)	1690, 1694, 1975
BceAI	ACGGC	4 cut(s)	192, 202, 400, 1445
BcgI	CGANNNNNNTGC	4 cut(s)	504, 538, 1366, 1400
BciT130I	CCWGG	5 cut(s)	252, 1932, 1956, 1976, 1989
BcnI	CCSGG	1 cut(s)	397
BcoDI	GTCTC	4 cut(s)	149, 1322, 1366, 1590
BcuI	ACTAGT	1 cut(s)	2015
BfaI	CTAG	2 cut(s)	792, 2016
BfmI	CTRYAG	5 cut(s)	308, 1542, 1633, 1854, 2022
BfuAI	ACCTGC	1 cut(s)	1091
BglII	AGATCT	1 cut(s)	312
BisI	GCNGC	5 cut(s)	728, 912, 1062, 1684, 2002
BlsI	GCNGC	5 cut(s)	729, 913, 1063, 1685, 2003
BmcAI	AGTACT	1 cut(s)	124
Bme1390I	CCNGG	6 cut(s)	252, 397, 1932, 1956, 1976, 1989
Bme18I	GGWCC	2 cut(s)	1534, 1952
BmeT110I	CYCGRG	2 cut(s)	1256, 1398
BmgT120I	GGNCC	2 cut(s)	1534, 1952
BmiI	GGNNCC	4 cut(s)	414, 715, 1570, 1986
BmrFI	CCNGG	6 cut(s)	252, 397, 1932, 1956, 1976, 1989
BmsI	GCATC	3 cut(s)	524, 609, 1468
BpiI	GAAGAC	1 cut(s)	462
BpmI	CTGGAG	2 cut(s)	234, 615
Bpu10I	CCTNAGC	1 cut(s)	1923
BpuEI	CTTGAG	2 cut(s)	686, 1676
BpuMI	CCSGG	1 cut(s)	397
BsaBI	GATNNNNATC	1 cut(s)	654
BsaHI	GRCGYC	2 cut(s)	842, 1629
BsaJI	CCNNGG	4 cut(s)	234, 508, 1270, 1975
BsaWI	WCCGGW	2 cut(s)	592, 744
Bsc4I	CCNNNNNNNGG	3 cut(s)	396, 599, 1981
Bse118I	RCCGGY	1 cut(s)	592
Bse1I	ACTGG	1 cut(s)	1367
Bse3DI	GCAATG	1 cut(s)	629
Bse8I	GATNNNNATC	1 cut(s)	654
BseBI	CCWGG	5 cut(s)	252, 1932, 1956, 1976, 1989
BseDI	CCNNGG	4 cut(s)	234, 508, 1270, 1975
BseGI	GGATG	3 cut(s)	246, 583, 1705
BseJI	GATNNNNATC	1 cut(s)	654
BseLI	CCNNNNNNNGG	3 cut(s)	396, 599, 1981
BseMI	GCAATG	1 cut(s)	629
BseMII	CTCAG	5 cut(s)	194, 909, 1071, 1581, 1937
BseNI	ACTGG	1 cut(s)	1367
BseRI	GAGGAG	3 cut(s)	137, 140, 798
BseXI	GCAGC	3 cut(s)	1073, 1695, 2013
BsgI	GTGCAG	2 cut(s)	60, 1119
Bsh1236I	CGCG	1 cut(s)	911
BshFI	GGCC	2 cut(s)	730, 1608
BshNI	GGYRCC	2 cut(s)	713, 1984
BshTI	ACCGGT	1 cut(s)	592
BsiHKAI	GWGCWC	2 cut(s)	1767, 1893
BsiHKCI	CYCGRG	2 cut(s)	1256, 1398
BsiSI	CCGG	3 cut(s)	396, 593, 745
BslFI	GGGAC	1 cut(s)	1156
BslI	CCNNNNNNNGG	3 cut(s)	396, 599, 1981
BsmAI	GTCTC	4 cut(s)	149, 1322, 1366, 1590
BsmBI	CGTCTC	2 cut(s)	149, 1366
BsmFI	GGGAC	1 cut(s)	1156
BsnI	GGCC	2 cut(s)	730, 1608
BsoBI	CYCGRG	2 cut(s)	1256, 1398
Bsp1286I	GDGCHC	2 cut(s)	1767, 1893
Bsp143I	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
Bsp19I	CCATGG	1 cut(s)	234
BspACI	CCGC	9 cut(s)	727, 803, 818, 827, 911, 1025, 1154, 1481, 1554
BspANI	GGCC	2 cut(s)	730, 1608
BspCNI	CTCAG	5 cut(s)	193, 908, 1072, 1582, 1936
BspFNI	CGCG	1 cut(s)	911
BspLI	GGNNCC	4 cut(s)	414, 715, 1570, 1986
BspMAI	CTGCAG	1 cut(s)	1858
BspMI	ACCTGC	1 cut(s)	1091
BspPI	GGATC	3 cut(s)	1529, 1766, 1966
BspT107I	GGYRCC	2 cut(s)	713, 1984
BsrDI	GCAATG	1 cut(s)	629
BsrFI	RCCGGY	1 cut(s)	592
BsrI	ACTGG	1 cut(s)	1367
BssAI	RCCGGY	1 cut(s)	592
BssECI	CCNNGG	4 cut(s)	234, 508, 1270, 1975
BssMI	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
BssNAI	GTATAC	1 cut(s)	759
BssNI	GRCGYC	2 cut(s)	842, 1629
BssT1I	CCWWGG	3 cut(s)	234, 508, 1270
Bst1107I	GTATAC	1 cut(s)	759
Bst2UI	CCWGG	5 cut(s)	252, 1932, 1956, 1976, 1989
Bst4CI	ACNGT	5 cut(s)	140, 281, 335, 1369, 1543
Bst6I	CTCTTC	2 cut(s)	701, 1272
BstACI	GRCGYC	2 cut(s)	842, 1629
BstC8I	GCNNGC	1 cut(s)	818
BstDEI	CTNAG	7 cut(s)	180, 895, 1080, 1474, 1590, 1705, 1923
BstDSI	CCRYGG	1 cut(s)	234
BstF5I	GGATG	3 cut(s)	246, 583, 1705
BstFNI	CGCG	1 cut(s)	911
BstHHI	GCGC	1 cut(s)	248
BstKTI	GATC	6 cut(s)	315, 1524, 1662, 1774, 1861, 1961
BstMAI	GTCTC	4 cut(s)	149, 1322, 1366, 1590
BstMBI	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
BstMWI	GCNNNNNNNGC	4 cut(s)	254, 826, 1090, 1553
BstNI	CCWGG	5 cut(s)	252, 1932, 1956, 1976, 1989
BstNSI	RCATGY	1 cut(s)	1585
BstSCI	CCNGG	6 cut(s)	250, 395, 1930, 1954, 1974, 1987
BstSFI	CTRYAG	5 cut(s)	308, 1542, 1633, 1854, 2022
BstUI	CGCG	1 cut(s)	911
BstV1I	GCAGC	3 cut(s)	1073, 1695, 2013
BstV2I	GAAGAC	1 cut(s)	462
BstX2I	RGATCY	3 cut(s)	312, 1521, 1771
BstYI	RGATCY	3 cut(s)	312, 1521, 1771
BstZ17I	GTATAC	1 cut(s)	759
BsuRI	GGCC	2 cut(s)	730, 1608
BtgI	CCRYGG	1 cut(s)	234
BtgZI	GCGATG	1 cut(s)	1815
BtsCI	GGATG	3 cut(s)	246, 583, 1705
BtsI	GCAGTG	1 cut(s)	96
BtsIMutI	CAGTG	2 cut(s)	96, 1374
BveI	ACCTGC	1 cut(s)	1091
Cac8I	GCNNGC	1 cut(s)	818
CaiI	CAGNNNCTG	1 cut(s)	257
CfoI	GCGC	1 cut(s)	248
Cfr10I	RCCGGY	1 cut(s)	592
Cfr13I	GGNCC	2 cut(s)	1534, 1952
CseI	GACGC	2 cut(s)	850, 1383
Csp6I	GTAC	4 cut(s)	123, 216, 501, 697
CspAI	ACCGGT	1 cut(s)	592
CviAII	CATG	5 cut(s)	235, 342, 1582, 1604, 1760
CviQI	GTAC	4 cut(s)	123, 216, 501, 697
DdeI	CTNAG	7 cut(s)	180, 895, 1080, 1474, 1590, 1705, 1923
DpnI	GATC	6 cut(s)	314, 1523, 1661, 1773, 1860, 1960
DpnII	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
DraI	TTTAAA	3 cut(s)	837, 1017, 1047
DraIII	CACNNNGTG	1 cut(s)	1888
EaeI	YGGCCR	2 cut(s)	728, 1606
Eam1104I	CTCTTC	2 cut(s)	701, 1272
EarI	CTCTTC	2 cut(s)	701, 1272
Ecl136II	GAGCTC	1 cut(s)	1765
Eco130I	CCWWGG	3 cut(s)	234, 508, 1270
Eco24I	GRGCYC	1 cut(s)	1767
Eco47I	GGWCC	2 cut(s)	1534, 1952
Eco53kI	GAGCTC	1 cut(s)	1765
Eco57I	CTGAAG	2 cut(s)	40, 1927
Eco88I	CYCGRG	2 cut(s)	1256, 1398
EcoICRI	GAGCTC	1 cut(s)	1765
EcoRI	GAATTC	1 cut(s)	1404
EcoRII	CCWGG	5 cut(s)	250, 1930, 1954, 1974, 1987
EcoT14I	CCWWGG	3 cut(s)	234, 508, 1270
EcoT22I	ATGCAT	1 cut(s)	1761
EcoT38I	GRGCYC	1 cut(s)	1767
ErhI	CCWWGG	3 cut(s)	234, 508, 1270
Esp3I	CGTCTC	2 cut(s)	149, 1366
FaeI	CATG	5 cut(s)	238, 345, 1585, 1607, 1763
FalI	AAGNNNNNCTT	2 cut(s)	345, 377
FaqI	GGGAC	1 cut(s)	1156
FatI	CATG	5 cut(s)	234, 341, 1581, 1603, 1759
FauI	CCCGC	1 cut(s)	825
FauNDI	CATATG	1 cut(s)	1726
FblI	GTMKAC	3 cut(s)	228, 758, 1874
Fnu4HI	GCNGC	5 cut(s)	728, 912, 1062, 1684, 2002
FokI	GGATG	3 cut(s)	253, 590, 1712
FriOI	GRGCYC	1 cut(s)	1767
Fsp4HI	GCNGC	5 cut(s)	728, 912, 1062, 1684, 2002
FspBI	CTAG	2 cut(s)	792, 2016
GlaI	GCGC	1 cut(s)	247
GluI	GCNGC	5 cut(s)	728, 912, 1062, 1684, 2002
GsuI	CTGGAG	2 cut(s)	234, 615
HaeIII	GGCC	2 cut(s)	730, 1608
HapII	CCGG	3 cut(s)	396, 593, 745
HgaI	GACGC	2 cut(s)	850, 1383
HhaI	GCGC	1 cut(s)	248
Hin1I	GRCGYC	2 cut(s)	842, 1629
Hin1II	CATG	5 cut(s)	238, 345, 1585, 1607, 1763
Hin6I	GCGC	1 cut(s)	246
HinP1I	GCGC	1 cut(s)	246
HincII	GTYRAC	1 cut(s)	1711
HindII	GTYRAC	1 cut(s)	1711
HindIII	AAGCTT	3 cut(s)	52, 1091, 1226
HinfI	GANTC	6 cut(s)	225, 891, 1028, 1424, 1651, 1879
HpaI	GTTAAC	1 cut(s)	1711
HpaII	CCGG	3 cut(s)	396, 593, 745
HphI	GGTGA	8 cut(s)	362, 538, 628, 816, 1124, 1199, 1874, 2032
Hpy166II	GTNNAC	9 cut(s)	229, 305, 439, 598, 759, 1120, 1534, 1711, 1875
Hpy188I	TCNGA	5 cut(s)	183, 1081, 1511, 1627, 1695
Hpy8I	GTNNAC	9 cut(s)	229, 305, 439, 598, 759, 1120, 1534, 1711, 1875
Hpy99I	CGWCG	1 cut(s)	144
HpyAV	CCTTC	4 cut(s)	169, 400, 457, 855
HpyCH4III	ACNGT	5 cut(s)	140, 281, 335, 1369, 1543
HpyCH4IV	ACGT	3 cut(s)	171, 1629, 1818
HpyCH4V	TGCA	9 cut(s)	77, 572, 622, 627, 972, 1100, 1223, 1759, 1856
HpyF10VI	GCNNNNNNNGC	4 cut(s)	254, 826, 1090, 1553
HpyF3I	CTNAG	7 cut(s)	180, 895, 1080, 1474, 1590, 1705, 1923
HpySE526I	ACGT	3 cut(s)	171, 1629, 1818
Hsp92I	GRCGYC	2 cut(s)	842, 1629
Hsp92II	CATG	5 cut(s)	238, 345, 1585, 1607, 1763
HspAI	GCGC	1 cut(s)	246
KspAI	GTTAAC	1 cut(s)	1711
Kzo9I	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
LmnI	GCTCC	6 cut(s)	253, 278, 412, 512, 589, 1272
Lsp1109I	GCAGC	3 cut(s)	1073, 1695, 2013
LweI	GCATC	3 cut(s)	524, 609, 1468
MaeI	CTAG	2 cut(s)	792, 2016
MaeII	ACGT	3 cut(s)	171, 1629, 1818
MalI	GATC	6 cut(s)	314, 1523, 1661, 1773, 1860, 1960
MboI	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
MfeI	CAATTG	1 cut(s)	1053
MflI	RGATCY	3 cut(s)	312, 1521, 1771
MhlI	GDGCHC	2 cut(s)	1767, 1893
MlsI	TGGCCA	1 cut(s)	1608
MluNI	TGGCCA	1 cut(s)	1608
MlyI	GAGTC	1 cut(s)	234
MmeI	TCCRAC	3 cut(s)	300, 1338, 1546
Mox20I	TGGCCA	1 cut(s)	1608
Mph1103I	ATGCAT	1 cut(s)	1761
MroXI	GAANNNNTTC	3 cut(s)	535, 1032, 1302
MscI	TGGCCA	1 cut(s)	1608
MslI	CAYNNNNRTG	2 cut(s)	239, 611
Msp20I	TGGCCA	1 cut(s)	1608
MspI	CCGG	3 cut(s)	396, 593, 745
MspR9I	CCNGG	6 cut(s)	252, 397, 1932, 1956, 1976, 1989
MunI	CAATTG	1 cut(s)	1053
MvaI	CCWGG	5 cut(s)	252, 1932, 1956, 1976, 1989
MvnI	CGCG	1 cut(s)	911
MwoI	GCNNNNNNNGC	4 cut(s)	254, 826, 1090, 1553
NciI	CCSGG	1 cut(s)	397
NcoI	CCATGG	1 cut(s)	234
NdeI	CATATG	1 cut(s)	1726
NdeII	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
NlaIII	CATG	5 cut(s)	238, 345, 1585, 1607, 1763
NlaIV	GGNNCC	4 cut(s)	414, 715, 1570, 1986
NmuCI	GTSAC	5 cut(s)	89, 172, 1448, 1597, 1720
NsiI	ATGCAT	1 cut(s)	1761
NspI	RCATGY	1 cut(s)	1585
PaeR7I	CTCGAG	1 cut(s)	1398
PaqCI	CACCTGC	1 cut(s)	1091
PciI	ACATGT	1 cut(s)	1581
PcsI	WCGNNNNNNNCGW	1 cut(s)	168
PdmI	GAANNNNTTC	3 cut(s)	535, 1032, 1302
PfeI	GAWTC	5 cut(s)	891, 1028, 1424, 1651, 1879
PflFI	GACNNNGTC	1 cut(s)	224
PflMI	CCANNNNNTGG	1 cut(s)	1981
PinAI	ACCGGT	1 cut(s)	592
PkrI	GCNGC	5 cut(s)	729, 913, 1063, 1685, 2003
PleI	GAGTC	1 cut(s)	233
PpsI	GAGTC	1 cut(s)	233
PscI	ACATGT	1 cut(s)	1581
PshBI	ATTAAT	1 cut(s)	786
Psp124BI	GAGCTC	1 cut(s)	1767
Psp6I	CCWGG	5 cut(s)	250, 1930, 1954, 1974, 1987
PspGI	CCWGG	5 cut(s)	250, 1930, 1954, 1974, 1987
PspN4I	GGNNCC	4 cut(s)	414, 715, 1570, 1986
PspPI	GGNCC	2 cut(s)	1534, 1952
PspXI	VCTCGAGB	1 cut(s)	1398
PstI	CTGCAG	1 cut(s)	1858
PstNI	CAGNNNCTG	1 cut(s)	257
PsuI	RGATCY	3 cut(s)	312, 1521, 1771
PsyI	GACNNNGTC	1 cut(s)	224
RsaI	GTAC	4 cut(s)	124, 217, 502, 698
RsaNI	GTAC	4 cut(s)	123, 216, 501, 697
RseI	CAYNNNNRTG	2 cut(s)	239, 611
SacI	GAGCTC	1 cut(s)	1767
SatI	GCNGC	5 cut(s)	728, 912, 1062, 1684, 2002
Sau3AI	GATC	6 cut(s)	312, 1521, 1659, 1771, 1858, 1958
Sau96I	GGNCC	2 cut(s)	1534, 1952
ScaI	AGTACT	1 cut(s)	124
SchI	GAGTC	1 cut(s)	234
ScrFI	CCNGG	6 cut(s)	252, 397, 1932, 1956, 1976, 1989
SduI	GDGCHC	2 cut(s)	1767, 1893
SfaNI	GCATC	3 cut(s)	524, 609, 1468
SfcI	CTRYAG	5 cut(s)	308, 1542, 1633, 1854, 2022
Sfr274I	CTCGAG	1 cut(s)	1398
SinI	GGWCC	2 cut(s)	1534, 1952
SlaI	CTCGAG	1 cut(s)	1398
SmiMI	CAYNNNNRTG	2 cut(s)	239, 611
SmlI	CTYRAG	3 cut(s)	665, 1398, 1655
SmoI	CTYRAG	3 cut(s)	665, 1398, 1655
SpeI	ACTAGT	1 cut(s)	2015
SsiI	CCGC	9 cut(s)	727, 803, 818, 827, 911, 1025, 1154, 1481, 1554
SspI	AATATT	3 cut(s)	566, 868, 945
SspMI	CTAG	2 cut(s)	792, 2016
SstI	GAGCTC	1 cut(s)	1767
StyD4I	CCNGG	6 cut(s)	250, 395, 1930, 1954, 1974, 1987
StyI	CCWWGG	3 cut(s)	234, 508, 1270
TaaI	ACNGT	5 cut(s)	140, 281, 335, 1369, 1543
TaiI	ACGT	3 cut(s)	174, 1632, 1821
TaqI	TCGA	5 cut(s)	1320, 1399, 1861, 1961, 1997
TatI	WGTACW	1 cut(s)	122
TauI	GCSGC	2 cut(s)	730, 914
TfiI	GAWTC	5 cut(s)	891, 1028, 1424, 1651, 1879
TscAI	CASTG	2 cut(s)	103, 1374
TseFI	GTSAC	5 cut(s)	89, 172, 1448, 1597, 1720
TseI	GCWGC	3 cut(s)	1061, 1683, 2001
Tsp45I	GTSAC	5 cut(s)	89, 172, 1448, 1597, 1720
TspGWI	ACGGA	2 cut(s)	344, 1965
TspRI	CASTG	2 cut(s)	103, 1374
Tth111I	GACNNNGTC	1 cut(s)	224
Van91I	CCANNNNNTGG	1 cut(s)	1981
VpaK11BI	GGWCC	2 cut(s)	1534, 1952
VspI	ATTAAT	1 cut(s)	786
XapI	RAATTY	3 cut(s)	467, 1298, 1404
XceI	RCATGY	1 cut(s)	1585
XhoI	CTCGAG	1 cut(s)	1398
XmiI	GTMKAC	3 cut(s)	228, 758, 1874
XmnI	GAANNNNTTC	3 cut(s)	535, 1032, 1302
XspI	CTAG	2 cut(s)	792, 2016
ZraI	GACGTC	1 cut(s)	1630
ZrmI	AGTACT	1 cut(s)	124
Zsp2I	ATGCAT	1 cut(s)	1761

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.