Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0021963

Physical Location & Seq

Genomic Coordinates Forward (+)

7208643 .. 7213915

Gene Structure 5273 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G21222.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2496 bp

ATGAGTGAGCCAAAGGTCAGGCGTTGGGTTGGCAAATATGAGGTTGGAAGATTGATCGGTGAATGTAATTTTGGGAAACTGCGGAGTGCTGTTGACACAGAGACAGGAGACCCTGTGGCTTTGATGATCCTTGATAAAGACAAGGTTCTGAAGCATAAGATGGCTGAACAAATAAAGAGAGAAATTTCTATTATGAAGCTGATCAACCATCCAAATGTAGTGCAGCTTTATGAGGTTTTGGCGAGCAAGGCGAAGATATATATTGTCCTAGAGTTCATCAGTGGTGGAAAGCTTTTTGACAAGATTAAAAATGATGGGCGCATGAATGAAGACGAAGCACAGAGATATTTCCAGCAGCTTATCAATGCAGTTGATTACTGCCACAGCAGGGGAGTGTACCACAGAGACTTAAAGCCAGAAAATTTGCTTCTTGATGCGCAAGAGAATTTGAAAGTCGCTGAATTTGGATTGATTGCCTTGTCCCAACAAGCCGGGGGAGATGGCCTTCGTCACACTGCATGTGGAAATCCAGATTATGCTGCTCCTGAGGTTCTTAATGATCAAGGCTATGATGGGGCAAAGGCAGACTTGTGGTCCTGTGGAGTGATACTCTTTGTACTACTTGCTGGGTATTTGCCTTTCGAGGATTCTAGTCTCACGACGCTGTACAAAAAGATCTCATCTGCTGATTTTAGTTGTCCTCCTTGGCTCTCTTCTGGTGTTAAGAATTTGATCGTCCGAATCTTGGATCCAAACCCTATGACTCGTATAACAATTCCAGAGATTTTGGAAGATGTGTGGTTCAAGAAAGATTACAAGCCAGCTGTATTTGAAGAGAAGAAAGAAGCAAATTTGGCTGATGTGGAAGCTGTTTTCAAAGATTCTGAAGAAGGGAGAGTGCAACTGAGGTCATTTCCTTGTGTGATATGTTCAGGTGGTACTACTTGTGGTGATGTTCGTTCAAGGACCAAACTGATGAACGGCTTGATAGAAAGAGGGAGGCCTCAAGAAGCTCATTCCATTTTCAACACACTTATTGAGGAAGGCCACAAGCCAAGCCTCATCACATACACCACGCTTGTAACTGCATTGACCCGTCAAAAGCACTTCCACTCTCTCCTCTCCCTCATCTCCAAAGTCGAGAAAAACGGCCTTAAACCTGACACCATTCTCTTCAACGCCATCATCAACGCCTCTTCCGAGTCTGGAAATCTCGATCAAGCAATGAAGATTTTCGAAAAAATGAAGGAAAGTGGATGTAAACCCACAGCAAGTACATTTAATACTCTCATCAAAGGATATGGCAAAATTGGTAAGCTGGAGGAATCATCAAGACTCCTGGATATGATGTTACGGGATGAGATGCTCCAACCAAATGACAGAACATGTAACATACTTGTCCAGGCTTGGTGTAACCAGAGGAAGATTGAAGAAGCATGGAACATTGTATATAAGATGCAATCTTATGGGGTAAAACCTGATGTAGTTACGTTTAACACATTGGCAAAAGCGTATGCGCGGATCGGGTCAACATGTACAGCTGAGGATATGATCATACCAAGGATGTTACACAATAAAGTTAAACCGAATGTACGTACCTGCGGTACCATTGTGAATGGCTACTGTGAAGAGGGCAAGATGGAAGAAGCTTTGAGGTTTTTCTATAGAATGAAGGAACTTGGAGTTCATCCTAATCTGTTTGTTTTTAACTCTCTAATCAAAGGCTTCCTTAACATCAACGACATGGATGGAGTTGGTGAGGTGGTGGATCTGATGGAAGAATTTGGAGTAAAACCTGATGTGGTAACGTTTAGCACTCTAATGAATGCATGGAGTTCTGTAGGAGATATGAAAAGATGCGAAGAGATCTATACAGATATGTTGGAAGGAGGAATAGATCCTGATATTCATGCATTTAGTATCCTTGCCAAAGGGTATGCTCGAGCTGGGGAGCCCGAAAAAGCGGAGCAAATCTTGAATCAGATGAGAAAATTTGGTGTTCGCCCAAATGTAGTGATCTACACACAGATCATAAGCGGTTGGTGCAGTGCTGGTGAAATGAAGAAAGCGATGCAGGTATATAAGAAAATGTGTGGAATCGTTGGTTTATCGCCTAACTTGACTACTTACGAGACTCTTATATGGGGATTTGGGGAAGCGAAGCAGCCATGGAAAGCAGAAGAGCTTCTGAAAGACATGGAAGGGAAGAATGTTGTTCCCACAAGGAAAACTATGCAACTCATAGCTGATGGATGGAAATCTATTGGTGTCTCTAATAGTAATGATGCCAATACATTGGGCTCTTCCTTCTCAACTTCCTCTAAACTCAATATCCCAAATAATATCGCCTCGTCGAGAAGTCCATTGTTCTTAAAAGGAATGCCTGAAAAACCAAAGCTATGTATCAAGTCTCAGTTTGGTTTGAGGCAGACTCTTTTGGTGGTGTTGTGCAGAGACCAGATTGGGGAAGCCGGGAGATTTTGTAAGTTCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 Pkinase

Protein Analysis

831

Amino Acids

93.14

Weight (kDa)

8.2

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	13 - 268	4.9e-67	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	15 - 265	1.4e-34	Protein tyrosine and serine/threonine kinase
PPR_2	PF13041	323 - 365	1.7e-07	PPR repeat family
TPR_24	PF23276	325 - 414	9e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	337 - 471	1.2e-08	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	341 - 398	6.1e-09	Pentatricopeptide repeat domain
PPR_3	PF13812	376 - 436	3.2e-16	Pentatricopeptide repeat domain
PPR_1	PF12854	383 - 416	3.8e-07	PPR repeat
PPR_2	PF13041	387 - 436	1.2e-14	PPR repeat family
PPR	PF01535	391 - 420	7.9e-06	PPR repeat
TPR_24	PF23276	415 - 514	5.7e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	419 - 450	1.2e-08	PPR repeat
PPR_2	PF13041	422 - 472	1.6e-08	PPR repeat family
PPR	PF01535	425 - 453	7.8e-06	PPR repeat
PPR_3	PF13812	447 - 505	4.5e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	458 - 507	3.7e-12	PPR repeat family
PPR_3	PF13812	522 - 575	7.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	527 - 558	1.3e-07	PPR repeat
PPR_2	PF13041	529 - 577	7.8e-13	PPR repeat family
PPR	PF01535	533 - 562	5.5e-07	PPR repeat
PPR_long	PF17177	548 - 663	2.2e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	564 - 610	1.8e-09	PPR repeat family
PPR_3	PF13812	588 - 644	3.2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	599 - 648	9.8e-11	PPR repeat family
PPR_long	PF17177	619 - 698	2.6e-07	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	623 - 679	1.2e-08	Pentatricopeptide repeat domain
PPR_3	PF13812	658 - 718	2.8e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	666 - 697	2.5e-09	PPR repeat
PPR_2	PF13041	669 - 717	4.4e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0021963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21222 AT5G21222 AT5G21222 AT5G21222

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	438
Acc36I	ACCTGC	2 cut(s)	1607, 2065
Acc65I	GGTACC	1 cut(s)	1604
AccB1I	GGYRCC	1 cut(s)	1604
AccII	CGCG	1 cut(s)	1519
AciI	CCGC	5 cut(s)	82, 1519, 1602, 1964, 2037
AclI	AACGTT	1 cut(s)	1808
AclWI	GGATC	6 cut(s)	121, 743, 756, 1529, 1776, 1892
AcsI	RAATTY	8 cut(s)	183, 421, 445, 461, 727, 850, 1781, 1991
AcuI	CTGAAG	2 cut(s)	170, 906
AfaI	GTAC	9 cut(s)	398, 618, 668, 940, 1276, 1537, 1593, 1597, 1606
AfiI	CCNNNNNNNGG	3 cut(s)	388, 745, 2464
AflIII	ACRYGT	2 cut(s)	1385, 1532
AgsI	TTSAA	9 cut(s)	451, 805, 833, 877, 963, 1027, 1177, 1430, 1978
AhdI	GACNNNNNGTC	1 cut(s)	592
AjnI	CCWGG	2 cut(s)	1338, 1401
AjuI	GAANNNNNNNTTGG	2 cut(s)	54, 86
Alw26I	GTCTC	8 cut(s)	95, 102, 399, 659, 2126, 2275, 2415, 2448
AlwI	GGATC	6 cut(s)	121, 743, 756, 1529, 1776, 1892
Ama87I	CYCGRG	1 cut(s)	1941
AoxI	GGCC	4 cut(s)	502, 1001, 1045, 1150
ApeKI	GCWGC	4 cut(s)	223, 355, 539, 2164
ApoI	RAATTY	8 cut(s)	183, 421, 445, 461, 727, 850, 1781, 1991
Asp700I	GAANNNNTTC	2 cut(s)	1232, 2184
Asp718I	GGTACC	1 cut(s)	1604
AspLEI	GCGC	3 cut(s)	321, 439, 1519
AspS9I	GGNCC	2 cut(s)	594, 966
AsuC2I	CCSGG	2 cut(s)	493, 2473
AsuHPI	GGTGA	4 cut(s)	71, 962, 1769, 2066
AsuII	TTCGAA	1 cut(s)	1236
AvaI	CYCGRG	1 cut(s)	1941
AvaII	GGWCC	2 cut(s)	594, 966
AxyI	CCTNAGG	1 cut(s)	546
BaeI	ACNNNNGTAYC	2 cut(s)	1596, 1629
BamHI	GGATCC	1 cut(s)	748
BanI	GGYRCC	1 cut(s)	1604
BanII	GRGCYC	2 cut(s)	1956, 2303
BbsI	GAAGAC	1 cut(s)	336
BbvCI	CCTCAGC	1 cut(s)	1542
BbvI	GCAGC	4 cut(s)	235, 367, 526, 2176
BceAI	ACGGC	2 cut(s)	997, 1165
BciT130I	CCWGG	2 cut(s)	1340, 1403
BciVI	GTATCC	1 cut(s)	1931
BclI	TGATCA	3 cut(s)	201, 559, 1551
BcnI	CCSGG	2 cut(s)	493, 2473
BcoDI	GTCTC	8 cut(s)	95, 102, 399, 659, 2126, 2275, 2415, 2448
BfaI	CTAG	2 cut(s)	269, 651
BfmI	CTRYAG	2 cut(s)	1663, 1839
BfuAI	ACCTGC	2 cut(s)	1607, 2065
BfuI	GTATCC	1 cut(s)	1931
BglII	AGATCT	2 cut(s)	675, 1866
BisI	GCNGC	4 cut(s)	224, 356, 540, 2165
BlsI	GCNGC	4 cut(s)	225, 357, 541, 2166
Bme1390I	CCNGG	4 cut(s)	493, 1340, 1403, 2473
Bme18I	GGWCC	2 cut(s)	594, 966
BmeRI	GACNNNNNGTC	1 cut(s)	592
BmeT110I	CYCGRG	1 cut(s)	1941
BmgT120I	GGNCC	2 cut(s)	594, 966
BmiI	GGNNCC	3 cut(s)	750, 1606, 1953
BmrFI	CCNGG	4 cut(s)	493, 1340, 1403, 2473
BmsI	GCATC	6 cut(s)	424, 1353, 1446, 1847, 2061, 2275
BpiI	GAAGAC	1 cut(s)	336
BplI	GAGNNNNNCTC	4 cut(s)	594, 626, 2416, 2448
BpmI	CTGGAG	1 cut(s)	1340
Bpu10I	CCTNAGC	1 cut(s)	1542
Bpu14I	TTCGAA	1 cut(s)	1236
BpuEI	CTTGAG	1 cut(s)	990
BpuMI	CCSGG	2 cut(s)	493, 2473
BsaAI	YACGTR	1 cut(s)	1595
BsaBI	GATNNNNATC	1 cut(s)	2257
BsaI	GGTCTC	2 cut(s)	102, 2448
BsaJI	CCNNGG	4 cut(s)	492, 704, 1559, 2168
BsaXI	ACNNNNNCTCC	3 cut(s)	99, 129, 2467
Bsc4I	CCNNNNNNNGG	3 cut(s)	388, 745, 2464
Bse21I	CCTNAGG	1 cut(s)	546
Bse3DI	GCAATG	1 cut(s)	1230
Bse8I	GATNNNNATC	1 cut(s)	2257
BseBI	CCWGG	2 cut(s)	1340, 1403
BseDI	CCNNGG	4 cut(s)	492, 704, 1559, 2168
BseGI	GGATG	7 cut(s)	208, 1262, 1363, 1569, 1687, 1753, 2258
BseJI	GATNNNNATC	1 cut(s)	2257
BseLI	CCNNNNNNNGG	3 cut(s)	388, 745, 2464
BseMI	GCAATG	1 cut(s)	1230
BseMII	CTCAG	4 cut(s)	537, 896, 1533, 2426
BseRI	GAGGAG	1 cut(s)	1109
BseXI	GCAGC	4 cut(s)	235, 367, 526, 2176
BseYI	CCCAGC	2 cut(s)	626, 1946
BsgI	GTGCAG	3 cut(s)	242, 2065, 2470
Bsh1236I	CGCG	1 cut(s)	1519
BshFI	GGCC	4 cut(s)	504, 1003, 1047, 1152
BshNI	GGYRCC	1 cut(s)	1604
BsiHKCI	CYCGRG	1 cut(s)	1941
BsiSI	CCGG	2 cut(s)	492, 2472
BslFI	GGGAC	1 cut(s)	466
BslI	CCNNNNNNNGG	3 cut(s)	388, 745, 2464
BsmAI	GTCTC	8 cut(s)	95, 102, 399, 659, 2126, 2275, 2415, 2448
BsmFI	GGGAC	1 cut(s)	466
BsmI	GAATGC	2 cut(s)	1831, 2385
BsnI	GGCC	4 cut(s)	504, 1003, 1047, 1152
Bso31I	GGTCTC	2 cut(s)	102, 2448
BsoBI	CYCGRG	1 cut(s)	1941
Bsp119I	TTCGAA	1 cut(s)	1236
Bsp1286I	GDGCHC	2 cut(s)	1956, 2303
Bsp1407I	TGTACA	2 cut(s)	666, 1535
Bsp19I	CCATGG	1 cut(s)	2168
BspACI	CCGC	5 cut(s)	82, 1519, 1602, 1964, 2037
BspANI	GGCC	4 cut(s)	504, 1003, 1047, 1152
BspCNI	CTCAG	4 cut(s)	538, 897, 1534, 2425
BspFNI	CGCG	1 cut(s)	1519
BspLI	GGNNCC	3 cut(s)	750, 1606, 1953
BspMI	ACCTGC	2 cut(s)	1607, 2065
BspPI	GGATC	6 cut(s)	121, 743, 756, 1529, 1776, 1892
BspQI	GCTCTTC	2 cut(s)	2175, 2308
BspT104I	TTCGAA	1 cut(s)	1236
BspT107I	GGYRCC	1 cut(s)	1604
BspTNI	GGTCTC	2 cut(s)	102, 2448
BsrDI	GCAATG	1 cut(s)	1230
BsrGI	TGTACA	2 cut(s)	666, 1535
BssECI	CCNNGG	4 cut(s)	492, 704, 1559, 2168
BssT1I	CCWWGG	3 cut(s)	704, 1559, 2168
Bst2UI	CCWGG	2 cut(s)	1340, 1403
Bst4CI	ACNGT	1 cut(s)	1625
Bst6I	CTCTTC	8 cut(s)	718, 828, 1178, 1201, 1623, 1857, 2175, 2308
BstAUI	TGTACA	2 cut(s)	666, 1535
BstBAI	YACGTR	1 cut(s)	1595
BstBI	TTCGAA	1 cut(s)	1236
BstC8I	GCNNGC	2 cut(s)	244, 822
BstDEI	CTNAG	4 cut(s)	546, 905, 1542, 2412
BstDSI	CCRYGG	1 cut(s)	2168
BstF5I	GGATG	7 cut(s)	208, 1262, 1363, 1569, 1687, 1753, 2258
BstFNI	CGCG	1 cut(s)	1519
BstHHI	GCGC	3 cut(s)	321, 439, 1519
BstMAI	GTCTC	8 cut(s)	95, 102, 399, 659, 2126, 2275, 2415, 2448
BstMWI	GCNNNNNNNGC	3 cut(s)	248, 854, 2043
BstNI	CCWGG	2 cut(s)	1340, 1403
BstNSI	RCATGY	3 cut(s)	522, 1389, 1536
BstSCI	CCNGG	4 cut(s)	491, 1338, 1401, 2471
BstSFI	CTRYAG	2 cut(s)	1663, 1839
BstSNI	TACGTA	1 cut(s)	1595
BstUI	CGCG	1 cut(s)	1519
BstV1I	GCAGC	4 cut(s)	235, 367, 526, 2176
BstV2I	GAAGAC	1 cut(s)	336
BstX2I	RGATCY	5 cut(s)	675, 748, 1768, 1866, 1897
BstXI	CCANNNNNNTGG	1 cut(s)	2296
BstYI	RGATCY	5 cut(s)	675, 748, 1768, 1866, 1897
Bsu36I	CCTNAGG	1 cut(s)	546
BsuI	GTATCC	1 cut(s)	1931
BsuRI	GGCC	4 cut(s)	504, 1003, 1047, 1152
BtgI	CCRYGG	1 cut(s)	2168
BtgZI	GCGATG	1 cut(s)	2084
BtsCI	GGATG	7 cut(s)	208, 1262, 1363, 1569, 1687, 1753, 2258
BtsI	GCAGTG	2 cut(s)	513, 2053
BtsIMutI	CAGTG	3 cut(s)	286, 513, 2053
BveI	ACCTGC	2 cut(s)	1607, 2065
Cac8I	GCNNGC	2 cut(s)	244, 822
CfoI	GCGC	3 cut(s)	321, 439, 1519
Cfr13I	GGNCC	2 cut(s)	594, 966
CseI	GACGC	1 cut(s)	670
Csp6I	GTAC	9 cut(s)	397, 617, 667, 939, 1275, 1536, 1592, 1596, 1605
CviQI	GTAC	9 cut(s)	397, 617, 667, 939, 1275, 1536, 1592, 1596, 1605
DdeI	CTNAG	4 cut(s)	546, 905, 1542, 2412
DriI	GACNNNNNGTC	1 cut(s)	592
Eam1104I	CTCTTC	8 cut(s)	718, 828, 1178, 1201, 1623, 1857, 2175, 2308
Eam1105I	GACNNNNNGTC	1 cut(s)	592
EarI	CTCTTC	8 cut(s)	718, 828, 1178, 1201, 1623, 1857, 2175, 2308
Eco105I	TACGTA	1 cut(s)	1595
Eco130I	CCWWGG	3 cut(s)	704, 1559, 2168
Eco147I	AGGCCT	1 cut(s)	1003
Eco24I	GRGCYC	2 cut(s)	1956, 2303
Eco31I	GGTCTC	2 cut(s)	102, 2448
Eco47I	GGWCC	2 cut(s)	594, 966
Eco57I	CTGAAG	2 cut(s)	170, 906
Eco81I	CCTNAGG	1 cut(s)	546
Eco88I	CYCGRG	1 cut(s)	1941
EcoRII	CCWGG	2 cut(s)	1338, 1401
EcoT14I	CCWWGG	3 cut(s)	704, 1559, 2168
EcoT22I	ATGCAT	2 cut(s)	1831, 1915
EcoT38I	GRGCYC	2 cut(s)	1956, 2303
ErhI	CCWWGG	3 cut(s)	704, 1559, 2168
FalI	AAGNNNNNCTT	4 cut(s)	572, 604, 1713, 1745
FaqI	GGGAC	1 cut(s)	466
FbaI	TGATCA	3 cut(s)	201, 559, 1551
Fnu4HI	GCNGC	4 cut(s)	224, 356, 540, 2165
FokI	GGATG	7 cut(s)	195, 1269, 1370, 1576, 1674, 1760, 2265
FriOI	GRGCYC	2 cut(s)	1956, 2303
Fsp4HI	GCNGC	4 cut(s)	224, 356, 540, 2165
FspBI	CTAG	2 cut(s)	269, 651
FspI	TGCGCA	1 cut(s)	438
GlaI	GCGC	3 cut(s)	320, 438, 1518
GluI	GCNGC	4 cut(s)	224, 356, 540, 2165
GsaI	CCCAGC	2 cut(s)	630, 1950
GsuI	CTGGAG	1 cut(s)	1340
HaeIII	GGCC	4 cut(s)	504, 1003, 1047, 1152
HapII	CCGG	2 cut(s)	492, 2472
HgaI	GACGC	1 cut(s)	670
HhaI	GCGC	3 cut(s)	321, 439, 1519
Hin6I	GCGC	3 cut(s)	319, 437, 1517
HinP1I	GCGC	3 cut(s)	319, 437, 1517
HincII	GTYRAC	2 cut(s)	94, 1530
HindII	GTYRAC	2 cut(s)	94, 1530
HindIII	AAGCTT	2 cut(s)	290, 1647
HpaII	CCGG	2 cut(s)	492, 2472
HphI	GGTGA	4 cut(s)	71, 962, 1769, 2066
Hpy166II	GTNNAC	4 cut(s)	94, 397, 1262, 1530
Hpy188I	TCNGA	7 cut(s)	150, 740, 886, 1201, 1773, 1983, 2190
Hpy8I	GTNNAC	4 cut(s)	94, 397, 1262, 1530
Hpy99I	CGWCG	2 cut(s)	664, 2356
HpyAV	CCTTC	8 cut(s)	515, 884, 1037, 1240, 1666, 1880, 2195, 2317
HpyCH4III	ACNGT	1 cut(s)	1625
HpyCH4IV	ACGT	3 cut(s)	1490, 1594, 1808
HpyF10VI	GCNNNNNNNGC	3 cut(s)	248, 854, 2043
HpyF3I	CTNAG	4 cut(s)	546, 905, 1542, 2412
HpySE526I	ACGT	3 cut(s)	1490, 1594, 1808
HspAI	GCGC	3 cut(s)	319, 437, 1517
KpnI	GGTACC	1 cut(s)	1608
Ksp22I	TGATCA	3 cut(s)	201, 559, 1551
LguI	GCTCTTC	2 cut(s)	2175, 2308
LmnI	GCTCC	4 cut(s)	547, 1371, 1951, 1966
Lsp1109I	GCAGC	4 cut(s)	235, 367, 526, 2176
LweI	GCATC	6 cut(s)	424, 1353, 1446, 1847, 2061, 2275
MaeI	CTAG	2 cut(s)	269, 651
MaeII	ACGT	3 cut(s)	1490, 1594, 1808
MaeIII	GTNAC	8 cut(s)	509, 1081, 1350, 1388, 1412, 1486, 1566, 1804
MflI	RGATCY	5 cut(s)	675, 748, 1768, 1866, 1897
MhlI	GDGCHC	2 cut(s)	1956, 2303
MlyI	GAGTC	5 cut(s)	757, 1211, 1329, 2128, 2425
MmeI	TCCRAC	3 cut(s)	25, 1393, 1863
Mph1103I	ATGCAT	2 cut(s)	1831, 1915
MroXI	GAANNNNTTC	2 cut(s)	1232, 2184
MslI	CAYNNNNRTG	2 cut(s)	213, 1820
MspA1I	CMGCKG	2 cut(s)	824, 1541
MspI	CCGG	2 cut(s)	492, 2472
MspR9I	CCNGG	4 cut(s)	493, 1340, 1403, 2473
Mva1269I	GAATGC	2 cut(s)	1831, 2385
MvaI	CCWGG	2 cut(s)	1340, 1403
MvnI	CGCG	1 cut(s)	1519
MwoI	GCNNNNNNNGC	3 cut(s)	248, 854, 2043
NciI	CCSGG	2 cut(s)	493, 2473
NcoI	CCATGG	1 cut(s)	2168
NlaIV	GGNNCC	3 cut(s)	750, 1606, 1953
NmuCI	GTSAC	1 cut(s)	509
NsbI	TGCGCA	1 cut(s)	438
NsiI	ATGCAT	2 cut(s)	1831, 1915
NspI	RCATGY	3 cut(s)	522, 1389, 1536
NspV	TTCGAA	1 cut(s)	1236
PaeR7I	CTCGAG	1 cut(s)	1941
PceI	AGGCCT	1 cut(s)	1003
PciI	ACATGT	2 cut(s)	1385, 1532
PciSI	GCTCTTC	2 cut(s)	2175, 2308
PcsI	WCGNNNNNNNCGW	1 cut(s)	1197
PctI	GAATGC	2 cut(s)	1831, 2385
PdmI	GAANNNNTTC	2 cut(s)	1232, 2184
PfeI	GAWTC	6 cut(s)	647, 741, 881, 1325, 1978, 2097
PfoI	TCCNGGA	1 cut(s)	1338
PkrI	GCNGC	4 cut(s)	225, 357, 541, 2166
PleI	GAGTC	5 cut(s)	757, 1210, 1329, 2128, 2425
PpsI	GAGTC	5 cut(s)	757, 1210, 1329, 2128, 2425
Ppu21I	YACGTR	1 cut(s)	1595
PscI	ACATGT	2 cut(s)	1385, 1532
Psp1406I	AACGTT	1 cut(s)	1808
Psp6I	CCWGG	2 cut(s)	1338, 1401
PspFI	CCCAGC	2 cut(s)	626, 1946
PspGI	CCWGG	2 cut(s)	1338, 1401
PspN4I	GGNNCC	3 cut(s)	750, 1606, 1953
PspPI	GGNCC	2 cut(s)	594, 966
PspXI	VCTCGAGB	1 cut(s)	1941
PsuI	RGATCY	5 cut(s)	675, 748, 1768, 1866, 1897
PvuII	CAGCTG	2 cut(s)	824, 1541
RsaI	GTAC	9 cut(s)	398, 618, 668, 940, 1276, 1537, 1593, 1597, 1606
RsaNI	GTAC	9 cut(s)	397, 617, 667, 939, 1275, 1536, 1592, 1596, 1605
RseI	CAYNNNNRTG	2 cut(s)	213, 1820
SapI	GCTCTTC	2 cut(s)	2175, 2308
SatI	GCNGC	4 cut(s)	224, 356, 540, 2165
Sau96I	GGNCC	2 cut(s)	594, 966
SchI	GAGTC	5 cut(s)	757, 1211, 1329, 2128, 2425
ScrFI	CCNGG	4 cut(s)	493, 1340, 1403, 2473
SduI	GDGCHC	2 cut(s)	1956, 2303
SfaNI	GCATC	6 cut(s)	424, 1353, 1446, 1847, 2061, 2275
SfcI	CTRYAG	2 cut(s)	1663, 1839
Sfr274I	CTCGAG	1 cut(s)	1941
SfuI	TTCGAA	1 cut(s)	1236
SinI	GGWCC	2 cut(s)	594, 966
SlaI	CTCGAG	1 cut(s)	1941
SmiMI	CAYNNNNRTG	2 cut(s)	213, 1820
SmlI	CTYRAG	2 cut(s)	1005, 1941
SmoI	CTYRAG	2 cut(s)	1005, 1941
SnaBI	TACGTA	1 cut(s)	1595
SseBI	AGGCCT	1 cut(s)	1003
SsiI	CCGC	5 cut(s)	82, 1519, 1602, 1964, 2037
SspMI	CTAG	2 cut(s)	269, 651
StuI	AGGCCT	1 cut(s)	1003
StyD4I	CCNGG	4 cut(s)	491, 1338, 1401, 2471
StyI	CCWWGG	3 cut(s)	704, 1559, 2168
TaaI	ACNGT	1 cut(s)	1625
TaiI	ACGT	3 cut(s)	1493, 1597, 1811
TaqI	TCGA	6 cut(s)	642, 1140, 1215, 1236, 1942, 2354
TatI	WGTACW	4 cut(s)	616, 666, 1274, 1535
TfiI	GAWTC	6 cut(s)	647, 741, 881, 1325, 1978, 2097
TscAI	CASTG	3 cut(s)	286, 520, 2053
TseFI	GTSAC	1 cut(s)	509
TseI	GCWGC	4 cut(s)	223, 355, 539, 2164
Tsp45I	GTSAC	1 cut(s)	509
TspRI	CASTG	3 cut(s)	286, 520, 2053
VpaK11BI	GGWCC	2 cut(s)	594, 966
XapI	RAATTY	8 cut(s)	183, 421, 445, 461, 727, 850, 1781, 1991
XceI	RCATGY	3 cut(s)	522, 1389, 1536
XhoI	CTCGAG	1 cut(s)	1941
XmnI	GAANNNNTTC	2 cut(s)	1232, 2184
XspI	CTAG	2 cut(s)	269, 651
Zsp2I	ATGCAT	2 cut(s)	1831, 1915

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.