AT5G25440
ERF Family

Protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0027363
Physical Location & Seq
Reverse (-)
8854507 .. 8856809
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25440.1

Sequence Viewer

Length: 942 bp
ATGGGAAATTGTCTCTCAGCTTCGACACTTCCCACCGAACCAAATGCTCCCAAATCAGTGGAGGAGGGTGTGAAAGTCTTCACGGTGGCAGATCTGAAGAAAGCGACTAATGATTTCGGAAACCAGATGGAACTAGGAGAGAGCTTGGGATACATCAATCCCAAAACGTTATCTCCGGCCAAGAAAGGTGTTGGCATGGCCGTCGCGGTCAAGAAAATTTACTTAGCCAATGAACAAGCTTTTCAAGATTGGCTTGTTGACGTAGAGTTTCTACGACATAACTCTCATCCAAGCTTGGTGAAGCTAATTGGCTACTGTTATGATCGTGATATGTTGTTTATTGTTTCTGAATACTTTCCAAATGGAAGCTTAGGAAGTTACATCAGTAGAGATAGTCGTCCAAAGTCATTGCCGTGGGAGACACGACTTAAGATATCCATAGGAGCGGCTCAGTGTCTTGCCTTCTTGCACTCTAGGAAGCAAGCCGGTCTATACCGCAGATATCTCACTGCTTCGAAAATCCTGCTCGACTCGGACTTCAACGCAAGAGTTTCCTATTTTGGAAAGCCAAAAGTTTCTCTCGATGAATTGGTATACACCATCGGGTTTTCAAATGTAGCACCTAGATATCAATACCCACCTCCCGAATACATACTCTCAGGGATGTCGAACATGGCGGGCGATGTGTATAGCTTTGGTGTTATACTACTTAAAATGTTAACTGGTTTAGGAAAAGACCTCACAATCTCAGCAAAGCGAGAAATAAAAAATAAAAAGTACAACATTGTGGAGATGATCGATCCAGATTTGAAGAATAGCTATCCACTTGAAGCGGGAAGACTTATGTGCGAGCTTATTAAACAATGCCTAGAGGTAGACCCGAAGATGCGACCAACCATGCAAGAAGTGCTAGATAATCTGAATGCTATAGCTCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

313

Amino Acids

35.0

Weight (kDa)

8.75

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 56 - 307 7.8e-31 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 62 - 305 5.2e-23 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027363)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25440 AT5G25440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 446
AccI GTMKAC 2 cut(s) 594, 876
AccII CGCG 1 cut(s) 206
AciI CCGC 5 cut(s) 206, 446, 496, 677, 833
AclI AACGTT 1 cut(s) 167
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 2 cut(s) 177, 198
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 1 cut(s) 116
AfaI GTAC 1 cut(s) 779
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 5 cut(s) 245, 541, 612, 811, 830
Alw26I GTCTC 2 cut(s) 17, 413
AlwI GGATC 1 cut(s) 794
AoxI GGCC 2 cut(s) 177, 198
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 2 cut(s) 77, 354
AsuHPI GGTGA 1 cut(s) 310
AsuII TTCGAA 1 cut(s) 515
BbsI GAAGAC 2 cut(s) 70, 844
BccI CCATC 2 cut(s) 121, 608
BceAI ACGGC 2 cut(s) 185, 397
BcgI CGANNNNNNTGC 6 cut(s) 26, 60, 184, 218, 505, 539
BciVI GTATCC 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 17, 413
BfaI CTAG 5 cut(s) 134, 474, 624, 869, 911
BfmI CTRYAG 1 cut(s) 927
BfrI CTTAAG 1 cut(s) 428
BfuI GTATCC 1 cut(s) 143
BglII AGATCT 1 cut(s) 91
BisI GCNGC 1 cut(s) 447
BlsI GCNGC 1 cut(s) 448
BmsI GCATC 1 cut(s) 876
BpiI GAAGAC 2 cut(s) 70, 844
Bpu10I CCTNAGC 1 cut(s) 370
Bpu14I TTCGAA 1 cut(s) 515
Bsa29I ATCGAT 1 cut(s) 798
BsaJI CCNNGG 1 cut(s) 413
BsaXI ACNNNNNCTCC 2 cut(s) 157, 187
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 1 cut(s) 727
Bse3DI GCAATG 1 cut(s) 407
BseCI ATCGAT 1 cut(s) 798
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 2 cut(s) 286, 669
BseMI GCAATG 1 cut(s) 407
BseMII CTCAG 4 cut(s) 30, 464, 672, 762
BseNI ACTGG 1 cut(s) 727
BseRI GAGGAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 179, 200
BshVI ATCGAT 1 cut(s) 798
BsiSI CCGG 2 cut(s) 176, 486
BsmAI GTCTC 2 cut(s) 17, 413
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 2 cut(s) 179, 200
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 4 cut(s) 91, 322, 795, 799
BspACI CCGC 5 cut(s) 206, 446, 496, 677, 833
BspANI GGCC 2 cut(s) 179, 200
BspCNI CTCAG 4 cut(s) 29, 463, 671, 761
BspDI ATCGAT 1 cut(s) 798
BspFNI CGCG 1 cut(s) 206
BspPI GGATC 1 cut(s) 794
BspT104I TTCGAA 1 cut(s) 515
BspTI CTTAAG 1 cut(s) 428
BsrBI CCGCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 407
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 1 cut(s) 727
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 4 cut(s) 91, 322, 795, 799
BssNAI GTATAC 1 cut(s) 595
Bst1107I GTATAC 1 cut(s) 595
Bst4CI ACNGT 2 cut(s) 85, 317
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 483, 679, 851
BstDEI CTNAG 7 cut(s) 16, 223, 370, 450, 658, 748, 933
BstDSI CCRYGG 1 cut(s) 413
BstF5I GGATG 2 cut(s) 286, 669
BstFNI CGCG 1 cut(s) 206
BstKTI GATC 4 cut(s) 94, 325, 798, 802
BstMAI GTCTC 2 cut(s) 17, 413
BstMBI GATC 4 cut(s) 91, 322, 795, 799
BstMWI GCNNNNNNNGC 1 cut(s) 907
BstSFI CTRYAG 1 cut(s) 927
BstUI CGCG 1 cut(s) 206
BstV2I GAAGAC 2 cut(s) 70, 844
BstX2I RGATCY 1 cut(s) 91
BstXI CCANNNNNNTGG 1 cut(s) 58
BstYI RGATCY 1 cut(s) 91
BstZ17I GTATAC 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 798
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 2 cut(s) 179, 200
BsuTUI ATCGAT 1 cut(s) 798
BtgI CCRYGG 1 cut(s) 413
BtgZI GCGATG 1 cut(s) 696
BtsCI GGATG 2 cut(s) 286, 669
BtsI GCAGTG 1 cut(s) 507
BtsIMutI CAGTG 3 cut(s) 63, 458, 507
Cac8I GCNNGC 3 cut(s) 483, 679, 851
Cfr10I RCCGGY 1 cut(s) 485
ClaI ATCGAT 1 cut(s) 798
Csp6I GTAC 1 cut(s) 778
CviAII CATG 3 cut(s) 196, 673, 898
CviQI GTAC 1 cut(s) 778
DdeI CTNAG 7 cut(s) 16, 223, 370, 450, 658, 748, 933
DpnI GATC 4 cut(s) 93, 324, 797, 801
DpnII GATC 4 cut(s) 91, 322, 795, 799
EaeI YGGCCR 2 cut(s) 177, 198
Eco32I GATATC 3 cut(s) 435, 503, 629
Eco57I CTGAAG 1 cut(s) 116
EcoRV GATATC 3 cut(s) 435, 503, 629
FaeI CATG 3 cut(s) 199, 676, 901
FalI AAGNNNNNCTT 2 cut(s) 237, 269
FatI CATG 3 cut(s) 195, 672, 897
FauI CCCGC 2 cut(s) 670, 826
FblI GTMKAC 2 cut(s) 594, 876
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 2 cut(s) 273, 676
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 5 cut(s) 134, 474, 624, 869, 911
GluI GCNGC 1 cut(s) 447
HaeIII GGCC 2 cut(s) 179, 200
HapII CCGG 2 cut(s) 176, 486
Hin1II CATG 3 cut(s) 199, 676, 901
HincII GTYRAC 2 cut(s) 259, 720
HindII GTYRAC 2 cut(s) 259, 720
HindIII AAGCTT 3 cut(s) 237, 292, 367
HinfI GANTC 1 cut(s) 530
HpaI GTTAAC 1 cut(s) 720
HpaII CCGG 2 cut(s) 176, 486
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 4 cut(s) 259, 595, 720, 877
Hpy188I TCNGA 6 cut(s) 96, 119, 349, 535, 921, 936
Hpy188III TCNNGA 6 cut(s) 211, 245, 326, 581, 644, 803
Hpy8I GTNNAC 4 cut(s) 259, 595, 720, 877
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 2 cut(s) 85, 317
HpyCH4IV ACGT 2 cut(s) 167, 261
HpyCH4V TGCA 2 cut(s) 469, 901
HpyF10VI GCNNNNNNNGC 1 cut(s) 907
HpyF3I CTNAG 7 cut(s) 16, 223, 370, 450, 658, 748, 933
HpySE526I ACGT 2 cut(s) 167, 261
Hsp92II CATG 3 cut(s) 199, 676, 901
KspAI GTTAAC 1 cut(s) 720
Kzo9I GATC 4 cut(s) 91, 322, 795, 799
LmnI GCTCC 2 cut(s) 52, 443
LpnPI CCDG 7 cut(s) 137, 189, 499, 536, 645, 708, 816
LweI GCATC 1 cut(s) 876
MaeI CTAG 5 cut(s) 134, 474, 624, 869, 911
MaeII ACGT 2 cut(s) 167, 261
MaeIII GTNAC 1 cut(s) 377
MalI GATC 4 cut(s) 93, 324, 797, 801
MbiI CCGCTC 1 cut(s) 446
MboI GATC 4 cut(s) 91, 322, 795, 799
MboII GAAGA 5 cut(s) 70, 109, 823, 849, 895
MflI RGATCY 1 cut(s) 91
MluCI AATT 4 cut(s) 7, 216, 306, 587
MlyI GAGTC 1 cut(s) 524
MnlI CCTC 5 cut(s) 55, 58, 651, 749, 865
MroXI GAANNNNTTC 2 cut(s) 77, 354
MseI TTAA 4 cut(s) 429, 711, 719, 858
MslI CAYNNNNRTG 1 cut(s) 412
MspCI CTTAAG 1 cut(s) 428
MspI CCGG 2 cut(s) 176, 486
Mva1269I GAATGC 1 cut(s) 928
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 1 cut(s) 907
NdeII GATC 4 cut(s) 91, 322, 795, 799
NlaIII CATG 3 cut(s) 199, 676, 901
NspV TTCGAA 1 cut(s) 515
PctI GAATGC 1 cut(s) 928
PdmI GAANNNNTTC 2 cut(s) 77, 354
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 1 cut(s) 524
PpsI GAGTC 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 167
PsuI RGATCY 1 cut(s) 91
RsaI GTAC 1 cut(s) 779
RsaNI GTAC 1 cut(s) 778
RseI CAYNNNNRTG 1 cut(s) 412
SaqAI TTAA 4 cut(s) 429, 711, 719, 858
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 4 cut(s) 91, 322, 795, 799
SchI GAGTC 1 cut(s) 524
SfaNI GCATC 1 cut(s) 876
SfcI CTRYAG 1 cut(s) 927
SfuI TTCGAA 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 4 cut(s) 7, 216, 306, 587
SsiI CCGC 5 cut(s) 206, 446, 496, 677, 833
SspMI CTAG 5 cut(s) 134, 474, 624, 869, 911
TaaI ACNGT 2 cut(s) 85, 317
TaiI ACGT 2 cut(s) 170, 264
TaqI TCGA 6 cut(s) 23, 515, 528, 582, 668, 798
TasI AATT 4 cut(s) 7, 216, 306, 587
TatI WGTACW 1 cut(s) 777
TauI GCSGC 1 cut(s) 449
Tru1I TTAA 4 cut(s) 429, 711, 719, 858
Tru9I TTAA 4 cut(s) 429, 711, 719, 858
TscAI CASTG 3 cut(s) 63, 458, 514
TspDTI ATGAA 2 cut(s) 246, 600
TspRI CASTG 3 cut(s) 63, 458, 514
Vha464I CTTAAG 1 cut(s) 428
XapI RAATTY 1 cut(s) 216
XmiI GTMKAC 2 cut(s) 594, 876
XmnI GAANNNNTTC 2 cut(s) 77, 354
XspI CTAG 5 cut(s) 134, 474, 624, 869, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.