AT5G28290

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020144
Physical Location & Seq
Reverse (-)
10278615 .. 10282824
4210 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28290.3

Sequence Viewer

Length: 1707 bp
ATGGAGCATTACGAGGTTCTTGAGCAAATTGGGAAAGGTTCTTTTGGGTCAGCTCTTCTTGTGAGGCATAAACATGAGAAGAAACTTTATGTTTTGAAGAAAATTCGGTTAGCTAGGCAGACTGGTAGAACAAGAAGGTCTGCTCATCAAGAGATGGAATTGATCTCAAAGATAAGGAATCCATTTATTGTGGAATATAAAGATTCTTGGGTTGAGAAAGGTTGCTATGTGTGCATTGTCATAGGCTATTGCAAAGGCGGTGACATGGCCGAAGCAATAAAGAAAGCCAACGGCGTAGAGTTCTCTGAAGAGAAACTCTGCAAGTGGCTGGTACAACTCTTAATGGCTCTTGAATACTTGCACGCCAGTCACATTCTCCATCGCGATGTCAAGTGTTCAAACATATTCTTGACAAAGGATCAAGATATACGCCTAGGTGATTTCGGACTTGCGAAGATCTTGACATCTGATGACCTTGCTTCTTCAGTGGTTGGAACGCCGAGCTACATGTGCCCTGAGCTTCTTGCTGATATTCCCTACGGGTCAAAGTCGGACATTTGGTCGCTGGGTTGTTGTATGTATGAGATGACGGCGCTGAAACCGGCATTCAAAGCCTTTGACATGCAAGGTCTGATCAACAGGATAAACCGGTCCATCGTTGCTCCACTTCCTGCGCAATATTCTACCGCCTTTCGCAGTCTCGTTAAAAGCATGCTCAGGAAGAATCCAGAACTCAGACCAAGCGCTTCTGATCTTCTCAGACAGCCACTACTTCAGCCCTACGTTCAAAAGGTTCTTCTCAAGCTGAGCTTCCGGGAACACGATACATTGCCATCTGAATCCGAAAGGAGAAGTAGCTATCCTCAGCAGAGAAAGCGTACTTCTGGCAAAAGTGTGAGCTTCGGTCCAAGCAGGTTCGGTGTTGACCAAGAAGATTCTGTTTCTTCTGTTAAACCCGTGCACACGTATTTACACCGTCACAGGCCAGTGGATTTGTCTGCAAATGACACTAGTAGAGTGGTTGTTCGTAGACCCGCTGTTTCTTCAGTGGTTTCTAACAGTTCAAAATATGTGCCAGTGAGATCAAACCAGCCTAAGAGTGGTGGTCTGCTGAAACCTGCGGTAGTGACTCGTAGGGCCTCCTTACCTATCTCACAGAAACCTGCAAAAGGAACAAAAGACTCTCTATACCACCCGAACATCGGTATACTCCATCAGCTAAATTCTCCAGATGTGTCAGTAAACTCGCCAAGAATCGACAGGATCAAGTTCCCGTTAGCTTCATATGAAGAAATGCCTTTCATTCCCGTTGTTCGCAAGAAGAAAGGATCGTCCAGAGGATCATACAGCCCGCCTCCAGAGCCACCACTAGACTGCTCAATAACAAAAGACAAATTCACACTTGAACCAGAACGAGAAACCAAAAGTGATTTGTCTGATCAAAATGCAACTGCTGGAGCATCAAGTAGAGCTTCCTCAGGTGCATCAAGAAGACAAAGATTCGACCCTAGCTCATATCGACAACGAGCTGAGGCACTTGAAGGACTACTTGAATTCAGTGCAAGATTACTTATAGACGAGAGGTACGATGAGCTGAACGTGTTACTAAAACCATTTGGTCCGGGAAAGGTATCGCCAAGAGAAACCGCGATATGGTTGTCTAAAAGCTTCAAGGAAAGTAGTCCTAGTAACTTGGAAGAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

568

Amino Acids

63.75

Weight (kDa)

9.53

Isoelectric Point (pI)

51.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 257 1.5e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 254 2.4e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04810 AT3G04810 AT3G04810 AT5G28290 AT5G28290 AT5G28290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 675
Acc36I ACCTGC 3 cut(s) 903, 1126, 1171
AccI GTMKAC 2 cut(s) 1030, 1207
AccII CGCG 2 cut(s) 384, 1649
AciI CCGC 6 cut(s) 258, 687, 1035, 1121, 1352, 1647
AclWI GGATC 4 cut(s) 426, 1271, 1336, 1348
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 4 cut(s) 102, 1222, 1394, 1553
AcuI CTGAAG 4 cut(s) 327, 468, 758, 1029
AfaI GTAC 3 cut(s) 333, 880, 1586
AfeI AGCGCT 1 cut(s) 745
AfiI CCNNNNNNNGG 4 cut(s) 1100, 1169, 1202, 1653
AflIII ACRYGT 3 cut(s) 507, 963, 1599
AgeI ACCGGT 1 cut(s) 648
AhdI GACNNNNNGTC 1 cut(s) 559
AhlI ACTAGT 1 cut(s) 1010
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 1 cut(s) 704
Alw44I GTGCAC 1 cut(s) 959
AlwI GGATC 4 cut(s) 426, 1271, 1336, 1348
Aor51HI AGCGCT 1 cut(s) 745
AoxI GGCC 3 cut(s) 267, 983, 1137
ApaLI GTGCAC 1 cut(s) 959
ApoI RAATTY 4 cut(s) 102, 1222, 1394, 1553
AsiGI ACCGGT 1 cut(s) 648
AspA2I CCTAGG 1 cut(s) 433
AspLEI GCGC 3 cut(s) 595, 676, 746
AspS9I GGNCC 4 cut(s) 651, 905, 1137, 1619
AsuC2I CCSGG 2 cut(s) 815, 1623
AsuHPI GGTGA 2 cut(s) 272, 449
AvaII GGWCC 3 cut(s) 651, 905, 1619
AvrII CCTAGG 1 cut(s) 433
AxyI CCTNAGG 1 cut(s) 1477
BaeGI GKGCMC 2 cut(s) 515, 963
BbsI GAAGAC 1 cut(s) 1498
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 2 cut(s) 864, 1530
BccI CCATC 5 cut(s) 148, 387, 662, 841, 1221
BceAI ACGGC 2 cut(s) 307, 606
BclI TGATCA 2 cut(s) 633, 1438
BcnI CCSGG 2 cut(s) 815, 1623
BcoDI GTCTC 1 cut(s) 704
BcuI ACTAGT 1 cut(s) 1010
BfaI CTAG 6 cut(s) 114, 434, 1011, 1370, 1509, 1686
BfoI RGCGCY 2 cut(s) 596, 747
BfuAI ACCTGC 3 cut(s) 903, 1126, 1171
BglII AGATCT 1 cut(s) 456
BlnI CCTAGG 1 cut(s) 433
BlpI GCTNAGC 1 cut(s) 806
Bme1390I CCNGG 2 cut(s) 815, 1623
Bme18I GGWCC 3 cut(s) 651, 905, 1619
BmeRI GACNNNNNGTC 1 cut(s) 559
BmgT120I GGNCC 4 cut(s) 651, 905, 1137, 1619
BmrFI CCNGG 2 cut(s) 815, 1623
BmsI GCATC 2 cut(s) 1469, 1493
BpiI GAAGAC 1 cut(s) 1498
BpmI CTGGAG 3 cut(s) 1212, 1341, 1476
Bpu10I CCTNAGC 4 cut(s) 516, 716, 864, 1530
Bpu1102I GCTNAGC 1 cut(s) 806
BpuEI CTTGAG 2 cut(s) 41, 785
BpuMI CCSGG 2 cut(s) 815, 1623
BsaAI YACGTR 1 cut(s) 966
BsaJI CCNNGG 1 cut(s) 433
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 4 cut(s) 1100, 1169, 1202, 1653
Bse118I RCCGGY 2 cut(s) 601, 648
Bse1I ACTGG 4 cut(s) 127, 366, 986, 1076
Bse21I CCTNAGG 1 cut(s) 1477
Bse3DI GCAATG 1 cut(s) 827
BseDI CCNNGG 1 cut(s) 433
BseLI CCNNNNNNNGG 4 cut(s) 1100, 1169, 1202, 1653
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 8 cut(s) 507, 730, 748, 772, 797, 878, 1491, 1521
BseNI ACTGG 4 cut(s) 127, 366, 986, 1076
BseSI GKGCMC 2 cut(s) 515, 963
BseYI CCCAGC 1 cut(s) 565
Bsh1236I CGCG 2 cut(s) 384, 1649
BshFI GGCC 3 cut(s) 269, 985, 1139
BshTI ACCGGT 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 963
BsiSI CCGG 4 cut(s) 602, 649, 814, 1622
BslI CCNNNNNNNGG 4 cut(s) 1100, 1169, 1202, 1653
BsmAI GTCTC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 3 cut(s) 269, 985, 1139
Bsp1286I GDGCHC 2 cut(s) 515, 963
Bsp1720I GCTNAGC 1 cut(s) 806
Bsp68I TCGCGA 1 cut(s) 384
BspACI CCGC 6 cut(s) 258, 687, 1035, 1121, 1352, 1647
BspANI GGCC 3 cut(s) 269, 985, 1139
BspCNI CTCAG 8 cut(s) 508, 729, 747, 771, 798, 877, 1490, 1522
BspFNI CGCG 2 cut(s) 384, 1649
BspMI ACCTGC 3 cut(s) 903, 1126, 1171
BspPI GGATC 4 cut(s) 426, 1271, 1336, 1348
BspQI GCTCTTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 827
BsrFI RCCGGY 2 cut(s) 601, 648
BsrI ACTGG 4 cut(s) 127, 366, 986, 1076
BssAI RCCGGY 2 cut(s) 601, 648
BssECI CCNNGG 1 cut(s) 433
BssNAI GTATAC 1 cut(s) 1208
BssT1I CCWWGG 1 cut(s) 433
Bst1107I GTATAC 1 cut(s) 1208
Bst4CI ACNGT 2 cut(s) 977, 1061
Bst6I CTCTTC 2 cut(s) 60, 303
BstBAI YACGTR 1 cut(s) 966
BstC8I GCNNGC 3 cut(s) 363, 713, 1352
BstDEI CTNAG 9 cut(s) 516, 716, 734, 758, 806, 864, 1095, 1477, 1530
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstFNI CGCG 2 cut(s) 384, 1649
BstH2I RGCGCY 2 cut(s) 596, 747
BstHHI GCGC 3 cut(s) 595, 676, 746
BstMAI GTCTC 1 cut(s) 704
BstMWI GCNNNNNNNGC 5 cut(s) 231, 510, 611, 874, 1360
BstNSI RCATGY 3 cut(s) 511, 625, 715
BstSCI CCNGG 2 cut(s) 813, 1621
BstSLI GKGCMC 2 cut(s) 515, 963
BstUI CGCG 2 cut(s) 384, 1649
BstV2I GAAGAC 1 cut(s) 1498
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BstZ17I GTATAC 1 cut(s) 1208
Bsu36I CCTNAGG 1 cut(s) 1477
BsuRI GGCC 3 cut(s) 269, 985, 1139
BtgZI GCGATG 2 cut(s) 365, 399
BtsIMutI CAGTG 5 cut(s) 492, 993, 1053, 1083, 1564
BtuMI TCGCGA 1 cut(s) 384
BveI ACCTGC 3 cut(s) 903, 1126, 1171
Cac8I GCNNGC 3 cut(s) 363, 713, 1352
CfoI GCGC 3 cut(s) 595, 676, 746
Cfr10I RCCGGY 2 cut(s) 601, 648
Cfr13I GGNCC 4 cut(s) 651, 905, 1137, 1619
Csp6I GTAC 3 cut(s) 332, 879, 1585
CspAI ACCGGT 1 cut(s) 648
CviAII CATG 5 cut(s) 74, 265, 508, 622, 712
CviQI GTAC 3 cut(s) 332, 879, 1585
DdeI CTNAG 9 cut(s) 516, 716, 734, 758, 806, 864, 1095, 1477, 1530
DriI GACNNNNNGTC 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 2 cut(s) 60, 303
Eam1105I GACNNNNNGTC 1 cut(s) 559
EarI CTCTTC 2 cut(s) 60, 303
Eco130I CCWWGG 1 cut(s) 433
Eco47I GGWCC 3 cut(s) 651, 905, 1619
Eco47III AGCGCT 1 cut(s) 745
Eco57I CTGAAG 4 cut(s) 327, 468, 758, 1029
Eco81I CCTNAGG 1 cut(s) 1477
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoO109I RGGNCCY 1 cut(s) 1137
EcoRI GAATTC 1 cut(s) 1553
EcoT14I CCWWGG 1 cut(s) 433
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 5 cut(s) 77, 268, 511, 625, 715
FalI AAGNNNNNCTT 6 cut(s) 794, 826, 1456, 1488, 1533, 1565
FatI CATG 5 cut(s) 73, 264, 507, 621, 711
FauI CCCGC 2 cut(s) 1042, 1359
FauNDI CATATG 1 cut(s) 1285
FbaI TGATCA 2 cut(s) 633, 1438
FblI GTMKAC 2 cut(s) 1030, 1207
FspBI CTAG 6 cut(s) 114, 434, 1011, 1370, 1509, 1686
FspI TGCGCA 1 cut(s) 675
GlaI GCGC 3 cut(s) 594, 675, 745
GsaI CCCAGC 1 cut(s) 569
GsuI CTGGAG 3 cut(s) 1212, 1341, 1476
HaeII RGCGCY 2 cut(s) 596, 747
HaeIII GGCC 3 cut(s) 269, 985, 1139
HapII CCGG 4 cut(s) 602, 649, 814, 1622
HhaI GCGC 3 cut(s) 595, 676, 746
Hin1II CATG 5 cut(s) 77, 268, 511, 625, 715
Hin6I GCGC 3 cut(s) 593, 674, 744
HinP1I GCGC 3 cut(s) 593, 674, 744
HincII GTYRAC 1 cut(s) 925
HindII GTYRAC 1 cut(s) 925
HindIII AAGCTT 1 cut(s) 1666
HinfI GANTC 9 cut(s) 178, 203, 724, 839, 935, 1129, 1181, 1254, 1500
HpaII CCGG 4 cut(s) 602, 649, 814, 1622
HphI GGTGA 2 cut(s) 272, 449
Hpy166II GTNNAC 5 cut(s) 925, 961, 1031, 1208, 1243
Hpy8I GTNNAC 5 cut(s) 925, 961, 1031, 1208, 1243
HpyAV CCTTC 2 cut(s) 129, 1535
HpyCH4III ACNGT 2 cut(s) 977, 1061
HpyCH4IV ACGT 3 cut(s) 783, 965, 1599
HpyF10VI GCNNNNNNNGC 5 cut(s) 231, 510, 611, 874, 1360
HpyF3I CTNAG 9 cut(s) 516, 716, 734, 758, 806, 864, 1095, 1477, 1530
HpySE526I ACGT 3 cut(s) 783, 965, 1599
Hsp92II CATG 5 cut(s) 77, 268, 511, 625, 715
HspAI GCGC 3 cut(s) 593, 674, 744
Ksp22I TGATCA 2 cut(s) 633, 1438
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 3 cut(s) 4, 667, 1457
LweI GCATC 2 cut(s) 1469, 1493
MaeI CTAG 6 cut(s) 114, 434, 1011, 1370, 1509, 1686
MaeII ACGT 3 cut(s) 783, 965, 1599
MaeIII GTNAC 6 cut(s) 260, 368, 977, 1126, 1602, 1688
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 515, 963
MluCI AATT 6 cut(s) 27, 102, 158, 1222, 1394, 1553
MlyI GAGTC 2 cut(s) 1123, 1175
MmeI TCCRAC 2 cut(s) 472, 531
MnlI CCTC 9 cut(s) 7, 57, 873, 1150, 1331, 1365, 1486, 1525, 1575
MseI TTAA 3 cut(s) 341, 705, 951
MslI CAYNNNNRTG 2 cut(s) 72, 384
MspA1I CMGCKG 1 cut(s) 1037
MspI CCGG 4 cut(s) 602, 649, 814, 1622
MspR9I CCNGG 2 cut(s) 815, 1623
Mva1269I GAATGC 1 cut(s) 605
MvnI CGCG 2 cut(s) 384, 1649
MwoI GCNNNNNNNGC 5 cut(s) 231, 510, 611, 874, 1360
NciI CCSGG 2 cut(s) 815, 1623
NdeI CATATG 1 cut(s) 1285
NlaIII CATG 5 cut(s) 77, 268, 511, 625, 715
NmeAIII GCCGAG 1 cut(s) 525
NmuCI GTSAC 4 cut(s) 260, 368, 977, 1126
NruI TCGCGA 1 cut(s) 384
NsbI TGCGCA 1 cut(s) 675
NspI RCATGY 3 cut(s) 511, 625, 715
PaeI GCATGC 1 cut(s) 715
PciI ACATGT 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 60
PcsI WCGNNNNNNNCGW 1 cut(s) 1584
PctI GAATGC 1 cut(s) 605
PfeI GAWTC 7 cut(s) 178, 203, 724, 839, 935, 1254, 1500
PfoI TCCNGGA 2 cut(s) 813, 1621
PinAI ACCGGT 1 cut(s) 648
PleI GAGTC 2 cut(s) 1123, 1175
PpsI GAGTC 2 cut(s) 1123, 1175
Ppu21I YACGTR 1 cut(s) 966
PscI ACATGT 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 565
PspPI GGNCC 4 cut(s) 651, 905, 1137, 1619
PsuI RGATCY 1 cut(s) 456
RruI TCGCGA 1 cut(s) 384
RsaI GTAC 3 cut(s) 333, 880, 1586
RsaNI GTAC 3 cut(s) 332, 879, 1585
RseI CAYNNNNRTG 2 cut(s) 72, 384
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 3 cut(s) 341, 705, 951
Sau96I GGNCC 4 cut(s) 651, 905, 1137, 1619
SchI GAGTC 2 cut(s) 1123, 1175
ScrFI CCNGG 2 cut(s) 815, 1623
SduI GDGCHC 2 cut(s) 515, 963
SfaNI GCATC 2 cut(s) 1469, 1493
SinI GGWCC 3 cut(s) 651, 905, 1619
SmiMI CAYNNNNRTG 2 cut(s) 72, 384
SmlI CTYRAG 2 cut(s) 20, 800
SmoI CTYRAG 2 cut(s) 20, 800
SpeI ACTAGT 1 cut(s) 1010
SphI GCATGC 1 cut(s) 715
Sse9I AATT 6 cut(s) 27, 102, 158, 1222, 1394, 1553
SsiI CCGC 6 cut(s) 258, 687, 1035, 1121, 1352, 1647
SspI AATATT 1 cut(s) 680
SspMI CTAG 6 cut(s) 114, 434, 1011, 1370, 1509, 1686
StyD4I CCNGG 2 cut(s) 813, 1621
StyI CCWWGG 1 cut(s) 433
TaaI ACNGT 2 cut(s) 977, 1061
TaiI ACGT 3 cut(s) 786, 968, 1602
TaqI TCGA 3 cut(s) 1257, 1503, 1519
TaqII GACCGA 1 cut(s) 893
TasI AATT 6 cut(s) 27, 102, 158, 1222, 1394, 1553
TfiI GAWTC 7 cut(s) 178, 203, 724, 839, 935, 1254, 1500
Tru1I TTAA 3 cut(s) 341, 705, 951
Tru9I TTAA 3 cut(s) 341, 705, 951
TscAI CASTG 5 cut(s) 492, 993, 1053, 1083, 1564
TseFI GTSAC 4 cut(s) 260, 368, 977, 1126
Tsp45I GTSAC 4 cut(s) 260, 368, 977, 1126
TspDTI ATGAA 3 cut(s) 1272, 1291, 1302
TspRI CASTG 5 cut(s) 492, 993, 1053, 1083, 1564
VneI GTGCAC 1 cut(s) 959
VpaK11BI GGWCC 3 cut(s) 651, 905, 1619
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 4 cut(s) 102, 1222, 1394, 1553
XceI RCATGY 3 cut(s) 511, 625, 715
XcmI CCANNNNNNNNNTGG 1 cut(s) 1097
XmaJI CCTAGG 1 cut(s) 433
XmiI GTMKAC 2 cut(s) 1030, 1207
XspI CTAG 6 cut(s) 114, 434, 1011, 1370, 1509, 1686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.