AT5G35550
MYB Family

Myb-like DNA-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0027287
Physical Location & Seq
Forward (+)
13726743 .. 13727961
1219 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35550.1

Sequence Viewer

Length: 777 bp
ATGGGAAAGAGAGCAACTACTAGTGTGAGGAGAGAAGAGTTAAACAGAGGAGCTTGGACTGATCATGAAGACAAGATCCTTAGAGATTACATCACCACTCACGGCGAAGGCAAATGGAGCACTCTCCCTAACCAAGCTGGTCTCAAGAGGTGTGGCAAAAGCTGTAGACTTCGGTGGAAGAACTACCTAAGACCGGGGATAAAGCGCGGTAACATCTCATCTGATGAAGAAGAACTCATAATCCGTCTCCATAATCTTCTTGGAAACAGATGGTCGTTGATAGCTGGGAGGCTTCCAGGCCGAACAGACAATGAAATAAAGAATCATTGGAACTCAAACCTCCGCAAAAGACTTCCCAAAACTCAAACCAAGCAACCAAAACGTATAAAACATTCGACGAACAACGAGAATAATGTATGTGTTATACGTACAAAGGCGATTAGGTGCTCAAAGACTCTTCTCTTCTCGGATCTCTCTCTTCAGAAGAAGAGTAGTACTAGTCCACTACCTCTGAAAGAACAAGAGATGGATCAAGGTGGATCTTCGTTGATGGGAGATCTCGAATTCGATTTCGATAGGATCCATTCGGAGTTTCACTTCCCGGATTTGATGGATTTTGATGGTTTGGACTGTGGAAACGTTACATCTCTTGTTTCATCTAACGAGATTTTGGGAGAGTTGGTTCCTGCTCAAGGTAATCTCGATCTCAATAGACCTTTCACTTCTTGTCATCATCGTGGCGACGATGAAGATTGGCTCCGAGACTTCACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006355 GO:0006357 GO:0006629 GO:0006631 GO:0006633 GO:0006725 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009628 GO:0009653 GO:0009698 GO:0009699 GO:0009791 GO:0009888 GO:0009889 GO:0009987 GO:0010023 GO:0010026 GO:0010090 GO:0010154 GO:0010191 GO:0010192 GO:0010214 GO:0010468 GO:0010556 GO:0016043 GO:0016053 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032787 GO:0032989 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0044550 GO:0046189 GO:0046394 GO:0046483 GO:0048316 GO:0048354 GO:0048359 GO:0048468 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0072330 GO:0080090 GO:0090558 GO:0090626 GO:0097159 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1903506 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

258

Amino Acids

29.61

Weight (kDa)

8.96

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 16 - 63 1.3e-14 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 19 - 77 7.5e-11 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 69 - 114 2e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 73 - 120 1e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0027287)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G35550 AT5G35550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 166
AccII CGCG 1 cut(s) 207
AciI CCGC 2 cut(s) 207, 343
AclI AACGTT 1 cut(s) 639
AclWI GGATC 6 cut(s) 70, 477, 537, 547, 574, 587
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 1 cut(s) 464
AfaI GTAC 2 cut(s) 430, 496
AfiI CCNNNNNNNGG 2 cut(s) 193, 692
AhlI ACTAGT 2 cut(s) 20, 497
AjnI CCWGG 1 cut(s) 295
AloI GAACNNNNNNTCC 4 cut(s) 225, 257, 666, 698
AluBI AGCT 4 cut(s) 53, 137, 162, 284
AluI AGCT 4 cut(s) 53, 137, 162, 284
Alw21I GWGCWC 2 cut(s) 122, 449
Alw26I GTCTC 3 cut(s) 146, 251, 756
AlwI GGATC 6 cut(s) 70, 477, 537, 547, 574, 587
AoxI GGCC 1 cut(s) 298
ApoI RAATTY 1 cut(s) 563
AspLEI GCGC 1 cut(s) 207
AsuC2I CCSGG 2 cut(s) 195, 602
AsuHPI GGTGA 1 cut(s) 85
BamHI GGATCC 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 75
Bbv12I GWGCWC 2 cut(s) 122, 449
BccI CCATC 5 cut(s) 264, 520, 544, 604, 614
BceAI ACGGC 1 cut(s) 118
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 61
BcnI CCSGG 2 cut(s) 195, 602
BcoDI GTCTC 3 cut(s) 146, 251, 756
BcuI ACTAGT 2 cut(s) 20, 497
BfaI CTAG 2 cut(s) 21, 498
BfmI CTRYAG 1 cut(s) 163
BglII AGATCT 1 cut(s) 556
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 3 cut(s) 195, 297, 602
BmiI GGNNCC 3 cut(s) 581, 684, 758
BmrFI CCNGG 3 cut(s) 195, 297, 602
BpiI GAAGAC 1 cut(s) 75
BpuEI CTTGAG 2 cut(s) 128, 675
BpuMI CCSGG 2 cut(s) 195, 602
BsaAI YACGTR 1 cut(s) 428
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 194
BsaXI ACNNNNNCTCC 2 cut(s) 666, 696
Bsc4I CCNNNNNNNGG 2 cut(s) 193, 692
BseBI CCWGG 1 cut(s) 297
BseDI CCNNGG 1 cut(s) 194
BseLI CCNNNNNNNGG 2 cut(s) 193, 692
BseRI GAGGAG 2 cut(s) 43, 63
BseYI CCCAGC 1 cut(s) 284
Bsh1236I CGCG 1 cut(s) 207
BshFI GGCC 1 cut(s) 300
BsiHKAI GWGCWC 2 cut(s) 122, 449
BsiSI CCGG 2 cut(s) 194, 602
BslI CCNNNNNNNGG 2 cut(s) 193, 692
BsmAI GTCTC 3 cut(s) 146, 251, 756
BsmBI CGTCTC 1 cut(s) 251
BsnI GGCC 1 cut(s) 300
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 2 cut(s) 122, 449
Bsp143I GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
BspACI CCGC 2 cut(s) 207, 343
BspANI GGCC 1 cut(s) 300
BspFNI CGCG 1 cut(s) 207
BspHI TCATGA 1 cut(s) 64
BspLI GGNNCC 3 cut(s) 581, 684, 758
BspPI GGATC 6 cut(s) 70, 477, 537, 547, 574, 587
BspTNI GGTCTC 1 cut(s) 146
BssECI CCNNGG 1 cut(s) 194
BssMI GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 632
Bst6I CTCTTC 5 cut(s) 30, 462, 467, 482, 483
BstBAI YACGTR 1 cut(s) 428
BstDEI CTNAG 2 cut(s) 80, 188
BstENI CCTNNNNNAGG 1 cut(s) 690
BstFNI CGCG 1 cut(s) 207
BstHHI GCGC 1 cut(s) 207
BstKTI GATC 8 cut(s) 64, 78, 472, 532, 542, 559, 582, 706
BstMAI GTCTC 3 cut(s) 146, 251, 756
BstMBI GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 1 cut(s) 297
BstSCI CCNGG 3 cut(s) 193, 295, 600
BstSFI CTRYAG 1 cut(s) 163
BstSNI TACGTA 1 cut(s) 428
BstUI CGCG 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 75
BstX2I RGATCY 5 cut(s) 75, 469, 539, 556, 579
BstYI RGATCY 5 cut(s) 75, 469, 539, 556, 579
BsuRI GGCC 1 cut(s) 300
CciI TCATGA 1 cut(s) 64
CfoI GCGC 1 cut(s) 207
Csp6I GTAC 2 cut(s) 429, 495
CspCI CAANNNNNGTGG 2 cut(s) 133, 168
CviAII CATG 1 cut(s) 65
CviJI RGCY 7 cut(s) 53, 137, 162, 284, 292, 300, 757
CviKI_1 RGCY 7 cut(s) 53, 137, 162, 284, 292, 300, 757
CviQI GTAC 2 cut(s) 429, 495
DdeI CTNAG 2 cut(s) 80, 188
DpnI GATC 8 cut(s) 63, 77, 471, 531, 541, 558, 581, 705
DpnII GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
Eam1104I CTCTTC 5 cut(s) 30, 462, 467, 482, 483
EarI CTCTTC 5 cut(s) 30, 462, 467, 482, 483
Eco105I TACGTA 1 cut(s) 428
Eco31I GGTCTC 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 464
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoRI GAATTC 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 295
Esp3I CGTCTC 1 cut(s) 251
FaeI CATG 1 cut(s) 68
FaiI YATR 6 cut(s) 66, 239, 252, 386, 418, 425
FatI CATG 1 cut(s) 64
FbaI TGATCA 1 cut(s) 61
FblI GTMKAC 1 cut(s) 166
FspBI CTAG 2 cut(s) 21, 498
GlaI GCGC 1 cut(s) 206
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 300
HapII CCGG 2 cut(s) 194, 602
HhaI GCGC 1 cut(s) 207
Hin1II CATG 1 cut(s) 68
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HinfI GANTC 2 cut(s) 322, 454
HpaII CCGG 2 cut(s) 194, 602
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 2 cut(s) 167, 503
Hpy188I TCNGA 6 cut(s) 223, 469, 483, 513, 589, 761
Hpy188III TCNNGA 4 cut(s) 65, 145, 560, 701
Hpy8I GTNNAC 2 cut(s) 167, 503
Hpy99I CGWCG 2 cut(s) 400, 746
HpyAV CCTTC 1 cut(s) 101
HpyCH4III ACNGT 1 cut(s) 632
HpyCH4IV ACGT 3 cut(s) 382, 427, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 2 cut(s) 80, 188
HpySE526I ACGT 3 cut(s) 382, 427, 639
Hsp92II CATG 1 cut(s) 68
HspAI GCGC 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
LmnI GCTCC 3 cut(s) 50, 117, 762
LpnPI CCDG 7 cut(s) 123, 207, 270, 282, 309, 615, 699
MaeI CTAG 2 cut(s) 21, 498
MaeII ACGT 3 cut(s) 382, 427, 639
MaeIII GTNAC 2 cut(s) 209, 640
MalI GATC 8 cut(s) 63, 77, 471, 531, 541, 558, 581, 705
MboI GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
MflI RGATCY 5 cut(s) 75, 469, 539, 556, 579
MhlI GDGCHC 2 cut(s) 122, 449
MluCI AATT 1 cut(s) 563
MlyI GAGTC 1 cut(s) 448
MnlI CCTC 6 cut(s) 21, 41, 141, 282, 350, 519
MseI TTAA 1 cut(s) 41
MslI CAYNNNNRTG 1 cut(s) 735
MspI CCGG 2 cut(s) 194, 602
MspR9I CCNGG 3 cut(s) 195, 297, 602
MvaI CCWGG 1 cut(s) 297
MvnI CGCG 1 cut(s) 207
MwoI GCNNNNNNNGC 1 cut(s) 117
NciI CCSGG 2 cut(s) 195, 602
NdeII GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
NlaIII CATG 1 cut(s) 68
NlaIV GGNNCC 3 cut(s) 581, 684, 758
PagI TCATGA 1 cut(s) 64
PfeI GAWTC 1 cut(s) 322
PfoI TCCNGGA 1 cut(s) 600
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Ppu21I YACGTR 1 cut(s) 428
Psp1406I AACGTT 1 cut(s) 639
Psp6I CCWGG 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 3 cut(s) 581, 684, 758
PsuI RGATCY 5 cut(s) 75, 469, 539, 556, 579
RsaI GTAC 2 cut(s) 430, 496
RsaNI GTAC 2 cut(s) 429, 495
RseI CAYNNNNRTG 1 cut(s) 735
SaqAI TTAA 1 cut(s) 41
Sau3AI GATC 8 cut(s) 61, 75, 469, 529, 539, 556, 579, 703
ScaI AGTACT 1 cut(s) 496
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 3 cut(s) 195, 297, 602
SduI GDGCHC 2 cut(s) 122, 449
SfcI CTRYAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 2 cut(s) 143, 690
SmoI CTYRAG 2 cut(s) 143, 690
SnaBI TACGTA 1 cut(s) 428
SpeI ACTAGT 2 cut(s) 20, 497
Sse9I AATT 1 cut(s) 563
SsiI CCGC 2 cut(s) 207, 343
SspMI CTAG 2 cut(s) 21, 498
StyD4I CCNGG 3 cut(s) 193, 295, 600
TaaI ACNGT 1 cut(s) 632
TaiI ACGT 3 cut(s) 385, 430, 642
TaqI TCGA 5 cut(s) 395, 561, 567, 573, 702
TasI AATT 1 cut(s) 563
TatI WGTACW 1 cut(s) 494
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 41
Tru9I TTAA 1 cut(s) 41
TspDTI ATGAA 5 cut(s) 81, 240, 327, 645, 762
TspGWI ACGGA 1 cut(s) 233
XagI CCTNNNNNAGG 1 cut(s) 690
XapI RAATTY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XmiI GTMKAC 1 cut(s) 166
XspI CTAG 2 cut(s) 21, 498
ZrmI AGTACT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.