AT5G43755

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0027087
Physical Location & Seq
Reverse (-)
17582977 .. 17583772
796 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G43755.1

Sequence Viewer

Length: 531 bp
ATGGAGAGCTTCGAACATTCCCCTTCCGAGTCTATCAAATCCCCAGATGCTACAAATGAAGCGGAAGAATGGATCACAAATGGCTTATGTGCGCAAGTTCCCACTCCGAGTACGACTAATGGCACAACTAGGCAGTTTCAGAGTCAGTGGCAAAAACCACATATGGGATGGATTAAGTGTAATTACGATGGCAGCTTTGTGAATCGAGTTCGTGGCTCTACGGCAGCTTGGATAATTAGAGATGATAACGGCGTGTTCAAAGGGGCTGCTCAAGCGACAGGCGACTGCAAGCTCCTAGTCAATGTTCTGAATGGAAGAGGCTCAAGATTTGATGCTATCAATTGGATCCATGAAATCAAAGTTTGGAGAAATAAGTTTGACGCAATCAAGTTCACGTGGTCACACAGATCAACAAATCAACCTGCTGATATTTTAGCGAAAAACCAGAAACATGCTCTGTCTTCATACATAGTTCATAGCTTTATCCCATTGTGTATCTCAAATGCTCTGTTTTCAGACTACGTTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Exo_endo_phos_2 RVT_3 zf-RVT

Protein Analysis

176

Amino Acids

19.81

Weight (kDa)

8.37

Isoelectric Point (pI)

23.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 94 - 148 9.4e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027087)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G43755
prunus_persica Prupe.4G264300_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 93
Acc36I ACCTGC 1 cut(s) 430
AciI CCGC 1 cut(s) 62
AclWI GGATC 3 cut(s) 80, 340, 353
AcvI CACGTG 1 cut(s) 396
AfaI GTAC 1 cut(s) 112
AfiI CCNNNNNNNGG 1 cut(s) 164
AgsI TTSAA 1 cut(s) 259
AluBI AGCT 5 cut(s) 9, 195, 227, 292, 480
AluI AGCT 5 cut(s) 9, 195, 227, 292, 480
AlwI GGATC 3 cut(s) 80, 340, 353
ApeKI GCWGC 3 cut(s) 192, 224, 266
AspLEI GCGC 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 12
BamHI GGATCC 1 cut(s) 345
BbrPI CACGTG 1 cut(s) 396
BbsI GAAGAC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 204, 236, 253
BccI CCATC 2 cut(s) 162, 182
BceAI ACGGC 2 cut(s) 237, 265
BfaI CTAG 2 cut(s) 129, 296
BfuAI ACCTGC 1 cut(s) 430
BisI GCNGC 3 cut(s) 193, 225, 267
BlsI GCNGC 3 cut(s) 194, 226, 268
BmiI GGNNCC 1 cut(s) 347
BmsI GCATC 2 cut(s) 37, 322
BpiI GAAGAC 1 cut(s) 453
Bpu14I TTCGAA 1 cut(s) 12
BpuEI CTTGAG 2 cut(s) 255, 307
BsaAI YACGTR 1 cut(s) 396
Bsc4I CCNNNNNNNGG 1 cut(s) 164
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 1 cut(s) 164
BseXI GCAGC 3 cut(s) 204, 236, 253
BslI CCNNNNNNNGG 1 cut(s) 164
Bsp119I TTCGAA 1 cut(s) 12
Bsp143I GATC 3 cut(s) 72, 345, 407
BspACI CCGC 1 cut(s) 62
BspLI GGNNCC 1 cut(s) 347
BspMI ACCTGC 1 cut(s) 430
BspPI GGATC 3 cut(s) 80, 340, 353
BspT104I TTCGAA 1 cut(s) 12
BssMI GATC 3 cut(s) 72, 345, 407
Bst6I CTCTTC 1 cut(s) 310
BstBAI YACGTR 1 cut(s) 396
BstBI TTCGAA 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 290
BstF5I GGATG 1 cut(s) 173
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 3 cut(s) 75, 348, 410
BstMBI GATC 3 cut(s) 72, 345, 407
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 455
BstV1I GCAGC 3 cut(s) 204, 236, 253
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 1 cut(s) 345
BstYI RGATCY 1 cut(s) 345
BtsCI GGATG 1 cut(s) 173
BtsIMutI CAGTG 1 cut(s) 152
BveI ACCTGC 1 cut(s) 430
Cac8I GCNNGC 1 cut(s) 290
CfoI GCGC 1 cut(s) 94
CseI GACGC 1 cut(s) 389
Csp6I GTAC 1 cut(s) 111
CviAII CATG 2 cut(s) 350, 452
CviJI RGCY 9 cut(s) 9, 84, 195, 216, 227, 266, 292, 321, 480
CviKI_1 RGCY 9 cut(s) 9, 84, 195, 216, 227, 266, 292, 321, 480
CviQI GTAC 1 cut(s) 111
DpnI GATC 3 cut(s) 74, 347, 409
DpnII GATC 3 cut(s) 72, 345, 407
Eam1104I CTCTTC 1 cut(s) 310
EarI CTCTTC 1 cut(s) 310
Eco72I CACGTG 1 cut(s) 396
FaeI CATG 2 cut(s) 353, 455
FaiI YATR 8 cut(s) 88, 162, 164, 351, 453, 466, 470, 477
FatI CATG 2 cut(s) 349, 451
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 3 cut(s) 193, 225, 267
FokI GGATG 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 193, 225, 267
FspBI CTAG 2 cut(s) 129, 296
FspI TGCGCA 1 cut(s) 93
GlaI GCGC 1 cut(s) 93
GluI GCNGC 3 cut(s) 193, 225, 267
HgaI GACGC 1 cut(s) 389
HhaI GCGC 1 cut(s) 94
Hin1II CATG 2 cut(s) 353, 455
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HinfI GANTC 3 cut(s) 29, 142, 202
Hpy166II GTNNAC 1 cut(s) 393
Hpy188I TCNGA 5 cut(s) 28, 108, 141, 309, 517
Hpy188III TCNNGA 1 cut(s) 324
Hpy8I GTNNAC 1 cut(s) 393
HpyAV CCTTC 1 cut(s) 33
HpyCH4IV ACGT 2 cut(s) 395, 522
HpyCH4V TGCA 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 395, 522
Hsp92II CATG 2 cut(s) 353, 455
HspAI GCGC 1 cut(s) 92
Kzo9I GATC 3 cut(s) 72, 345, 407
LmnI GCTCC 1 cut(s) 297
LpnPI CCDG 4 cut(s) 57, 264, 435, 458
Lsp1109I GCAGC 3 cut(s) 204, 236, 253
LweI GCATC 2 cut(s) 37, 322
MaeI CTAG 2 cut(s) 129, 296
MaeII ACGT 2 cut(s) 395, 522
MaeIII GTNAC 1 cut(s) 399
MalI GATC 3 cut(s) 74, 347, 409
MboI GATC 3 cut(s) 72, 345, 407
MboII GAAGA 3 cut(s) 77, 327, 453
MfeI CAATTG 1 cut(s) 340
MflI RGATCY 1 cut(s) 345
MluCI AATT 3 cut(s) 181, 234, 340
MlyI GAGTC 2 cut(s) 38, 151
MnlI CCTC 1 cut(s) 311
MseI TTAA 1 cut(s) 174
MunI CAATTG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeI CATATG 1 cut(s) 162
NdeII GATC 3 cut(s) 72, 345, 407
NlaIII CATG 2 cut(s) 353, 455
NlaIV GGNNCC 1 cut(s) 347
NmuCI GTSAC 1 cut(s) 399
NsbI TGCGCA 1 cut(s) 93
NspI RCATGY 1 cut(s) 455
NspV TTCGAA 1 cut(s) 12
PfeI GAWTC 1 cut(s) 202
PkrI GCNGC 3 cut(s) 194, 226, 268
PleI GAGTC 2 cut(s) 37, 150
PmaCI CACGTG 1 cut(s) 396
PmlI CACGTG 1 cut(s) 396
PpsI GAGTC 2 cut(s) 37, 150
Ppu21I YACGTR 1 cut(s) 396
PspCI CACGTG 1 cut(s) 396
PspN4I GGNNCC 1 cut(s) 347
PsuI RGATCY 1 cut(s) 345
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 3 cut(s) 193, 225, 267
Sau3AI GATC 3 cut(s) 72, 345, 407
SchI GAGTC 2 cut(s) 38, 151
SetI ASST 8 cut(s) 11, 197, 229, 294, 398, 424, 482, 525
SfaNI GCATC 2 cut(s) 37, 322
SfuI TTCGAA 1 cut(s) 12
SmlI CTYRAG 2 cut(s) 270, 322
SmoI CTYRAG 2 cut(s) 270, 322
Sse9I AATT 3 cut(s) 181, 234, 340
SsiI CCGC 1 cut(s) 62
SspMI CTAG 2 cut(s) 129, 296
TaiI ACGT 2 cut(s) 398, 525
TaqI TCGA 2 cut(s) 12, 205
TasI AATT 3 cut(s) 181, 234, 340
TfiI GAWTC 1 cut(s) 202
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 3 cut(s) 192, 224, 266
Tsp45I GTSAC 1 cut(s) 399
TspDTI ATGAA 4 cut(s) 72, 366, 453, 464
TspRI CASTG 1 cut(s) 152
XceI RCATGY 1 cut(s) 455
XcmI CCANNNNNNNNNTGG 1 cut(s) 165
XspI CTAG 2 cut(s) 129, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.