AT5G45220

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0026937
Physical Location & Seq
Reverse (-)
18298774 .. 18301166
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45220.1

Sequence Viewer

Length: 1641 bp
ATGGCGACTTTATCCTCTTCTTCTTCGGTAGAACTGCCACCGCAACATACAGTTTTCATCCTTTATAACGGATCACGCATGGGATTCATCTACCATCTCATCATGGCCTTGGAAAAAAAGAACATTAACGTCTTTGTGGGCTTCAATGGATGCATATGCGAACCAGTAGAAAGACTATCGAACAGAATAGAGTCGATAATCGTTCTGGTTATCTTCACCAGCAGGTACACAGAATCAAAATGGTGCTTGATGAAGCTGGTAGACATCAATAAATGTGCGGAGAAAGACCATCTGGTGGCTATTCCGATCTTCTACAAGTTGGATCCCTCCACCGTCAGAGGCCTGAGCGGACAGTTCGGTGATGCATTCAGGGATCTGAGAGAAAGTACTGGATTAATGGAAAAGTGGAAGGAAGCTTTGAAGTCGATTTCCGACAGACCGGGTATCAGGGTGGATAAGAGCAGCCCCAAGGCCAAGAGAATCGAAATAGTTGTGAAGAAGGTGTTGAGCAGAATTCCATCGGAGGGAAGCCATAACGCTTCTGTAGATCCATTAGAAAATAGTGACACTCGACTAACTTCCTCGGGCGAAGAAAATAGGACTTTTGGAATCAAAGAAAGACTGAAAGAATTTAAAGACAAGTTGGATCTCAATAAAATGAGAAGTCAGCTAGGAATGGTAATAGGTTGTCTTTTGGTTCTCTTTTTTGTTCTGAAACAAAGTGCTTATACGAGCACACGTTTAATAGGTGAGGGCGGTGCCATCGGTGCCATCAGTGCCATCGATAGAATTGCGGACAATGTACCAAAAATACGGGAGTACGTTCCTGAAGAGGGAACAGAGAAACCAAGAGACACATATACTGAATTTCATACTGGTAAATATGGAACAAAGCCCGAGAGATCTTCTTCTGACGTTAAAACAAATGCCGATCTCCTTGATAGAACTTTCACTGACACGCGAATGGACGGTAAAGTCAAGCCACCGAAATTTCAAGTTTTCATCAATTTCCGCGGAGATCAGTTGCGCAACAATTTCGTCGGCTATCTCGTTGATGCACTTCGAAGAAGTGAGATCAACGTTTTCATAGATAATCAGGAGCAAAGAGGTGAAGACCTCAACACTCTTTTCAAGAGGATCGAAGATTCTGGTATTGCCATAGTTGTGTTTTCGAGTAGGTACACAGAATCAAAATGGTGCTTGGAAGAGCTTGTGAAGATTAAAGAACGTGTGCACCAAGGCCTTCTCAAGGTGCTTCCCATCTTCTATAAGGTGACTCCAACCAATGTGAAAAGACCTAAGGGAGAGTTTGGTGACCATTTCAGAGATAAAGAGTACATGTACGAGTCTGATGAGCCTATGATAAAGCGATGGAAAGAGGCGATCGTGTTTATCTCCCATAGGTTTGCCCTGACTTTGGATGAAAAAAGTTCCTCGGAAATGTTAGACACATCGTTACATATGTATGTGCACGCTCGTGTGTGGAATTTGAAAGAACTCATCTCGACCTTTTTAACAAAGTGTAGCAGCGCATTGGAGATTGATTTTGTTGAAACTATCGTCAAGGAGGTCTTGAAAATTCTACAAGCCATATGCAAAGTGGAAAGTAGCCAATCCTCTTTTTGTGCGGAGAAAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TIR

Protein Analysis

546

Amino Acids

62.3

Weight (kDa)

8.71

Isoelectric Point (pI)

38.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 17 - 177 1.1e-24 TIR domain
TIR PF01582 330 - 481 2.6e-33 TIR domain
TIR_2 PF13676 333 - 426 1.3e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026937)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G45220 AT5G45220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AasI GACNNNNNNGTC 1 cut(s) 974
Acc16I TGCGCA 1 cut(s) 1028
Acc36I ACCTGC 1 cut(s) 213
AccB1I GGYRCC 2 cut(s) 758, 767
AccB7I CCANNNNNTGG 1 cut(s) 295
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 261
AccII CGCG 2 cut(s) 961, 1014
AciI CCGC 8 cut(s) 41, 278, 348, 756, 794, 1012, 1014, 1628
AclI AACGTT 1 cut(s) 1080
AclWI GGATC 7 cut(s) 79, 317, 330, 381, 542, 654, 1145
AcsI RAATTY 6 cut(s) 513, 629, 866, 989, 1486, 1578
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 7 cut(s) 227, 388, 804, 821, 1181, 1337, 1343
AfiI CCNNNNNNNGG 5 cut(s) 295, 524, 833, 1249, 1417
AflIII ACRYGT 3 cut(s) 737, 1228, 1338
AgsI TTSAA 7 cut(s) 145, 421, 995, 1132, 1492, 1553, 1576
AleI CACNNNNGTG 1 cut(s) 1476
AluBI AGCT 4 cut(s) 256, 416, 670, 1210
AluI AGCT 4 cut(s) 256, 416, 670, 1210
Alw21I GWGCWC 3 cut(s) 737, 1236, 1473
Alw26I GTCTC 1 cut(s) 846
Alw44I GTGCAC 2 cut(s) 1232, 1469
AlwI GGATC 7 cut(s) 79, 317, 330, 381, 542, 654, 1145
Ama87I CYCGRG 2 cut(s) 583, 896
AoxI GGCC 4 cut(s) 105, 340, 471, 1240
ApaLI GTGCAC 2 cut(s) 1232, 1469
ApeKI GCWGC 2 cut(s) 462, 1527
ApoI RAATTY 6 cut(s) 513, 629, 866, 989, 1486, 1578
AseI ATTAAT 1 cut(s) 395
AspLEI GCGC 2 cut(s) 1029, 1532
AsuC2I CCSGG 1 cut(s) 441
AsuHPI GGTGA 6 cut(s) 208, 371, 761, 1121, 1285, 1325
AsuII TTCGAA 1 cut(s) 1063
AvaI CYCGRG 2 cut(s) 583, 896
AxyI CCTNAGG 1 cut(s) 1299
BaeGI GKGCMC 2 cut(s) 1236, 1473
BamHI GGATCC 1 cut(s) 322
BanI GGYRCC 2 cut(s) 758, 767
BauI CACGAG 1 cut(s) 1476
BbsI GAAGAC 1 cut(s) 1119
Bbv12I GWGCWC 3 cut(s) 737, 1236, 1473
BbvI GCAGC 2 cut(s) 474, 1539
BccI CCATC 8 cut(s) 102, 297, 526, 770, 779, 788, 1268, 1365
BcgI CGANNNNNNTGC 4 cut(s) 773, 807, 1018, 1052
BcnI CCSGG 1 cut(s) 441
BcoDI GTCTC 1 cut(s) 846
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 543
BfuAI ACCTGC 1 cut(s) 213
BglII AGATCT 1 cut(s) 902
BisI GCNGC 2 cut(s) 463, 1528
BlsI GCNGC 2 cut(s) 464, 1529
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 1 cut(s) 441
BmeT110I CYCGRG 2 cut(s) 583, 896
BmiI GGNNCC 3 cut(s) 324, 760, 769
BmrFI CCNGG 1 cut(s) 441
BmsI GCATC 3 cut(s) 140, 352, 1045
BpiI GAAGAC 1 cut(s) 1119
Bpu10I CCTNAGC 1 cut(s) 344
Bpu14I TTCGAA 1 cut(s) 1063
BpuEI CTTGAG 1 cut(s) 1232
BpuMI CCSGG 1 cut(s) 441
Bsa29I ATCGAT 1 cut(s) 783
BsaJI CCNNGG 6 cut(s) 108, 468, 582, 1012, 1237, 1434
Bsc4I CCNNNNNNNGG 5 cut(s) 295, 524, 833, 1249, 1417
Bse1I ACTGG 3 cut(s) 164, 394, 880
Bse21I CCTNAGG 1 cut(s) 1299
BseCI ATCGAT 1 cut(s) 783
BseDI CCNNGG 6 cut(s) 108, 468, 582, 1012, 1237, 1434
BseGI GGATG 3 cut(s) 57, 155, 1426
BseLI CCNNNNNNNGG 5 cut(s) 295, 524, 833, 1249, 1417
BseMII CTCAG 2 cut(s) 335, 368
BseNI ACTGG 3 cut(s) 164, 394, 880
BseSI GKGCMC 2 cut(s) 1236, 1473
BseXI GCAGC 2 cut(s) 474, 1539
Bsh1236I CGCG 2 cut(s) 961, 1014
Bsh1285I CGRYCG 1 cut(s) 1386
BshFI GGCC 4 cut(s) 107, 342, 473, 1242
BshNI GGYRCC 2 cut(s) 758, 767
BshVI ATCGAT 1 cut(s) 783
BsiEI CGRYCG 1 cut(s) 1386
BsiHKAI GWGCWC 3 cut(s) 737, 1236, 1473
BsiHKCI CYCGRG 2 cut(s) 583, 896
BsiSI CCGG 1 cut(s) 440
BslI CCNNNNNNNGG 5 cut(s) 295, 524, 833, 1249, 1417
BsmAI GTCTC 1 cut(s) 846
BsmI GAATGC 1 cut(s) 365
BsnI GGCC 4 cut(s) 107, 342, 473, 1242
BsoBI CYCGRG 2 cut(s) 583, 896
Bsp119I TTCGAA 1 cut(s) 1063
Bsp1286I GDGCHC 3 cut(s) 737, 1236, 1473
BspACI CCGC 8 cut(s) 41, 278, 348, 756, 794, 1012, 1014, 1628
BspANI GGCC 4 cut(s) 107, 342, 473, 1242
BspCNI CTCAG 2 cut(s) 336, 369
BspDI ATCGAT 1 cut(s) 783
BspFNI CGCG 2 cut(s) 961, 1014
BspLI GGNNCC 3 cut(s) 324, 760, 769
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 7 cut(s) 79, 317, 330, 381, 542, 654, 1145
BspQI GCTCTTC 1 cut(s) 1200
BspT104I TTCGAA 1 cut(s) 1063
BspT107I GGYRCC 2 cut(s) 758, 767
BsrBI CCGCTC 1 cut(s) 348
BsrI ACTGG 3 cut(s) 164, 394, 880
BssECI CCNNGG 6 cut(s) 108, 468, 582, 1012, 1237, 1434
BssSI CACGAG 1 cut(s) 1476
BssT1I CCWWGG 3 cut(s) 108, 468, 1237
Bst2BI CACGAG 1 cut(s) 1476
Bst4CI ACNGT 4 cut(s) 52, 334, 354, 971
Bst6I CTCTTC 3 cut(s) 22, 825, 1200
BstBI TTCGAA 1 cut(s) 1063
BstC8I GCNNGC 1 cut(s) 1473
BstDEI CTNAG 3 cut(s) 344, 377, 1299
BstDSI CCRYGG 1 cut(s) 1012
BstEII GGTNACC 1 cut(s) 1313
BstENI CCTNNNNNAGG 1 cut(s) 1247
BstF5I GGATG 3 cut(s) 57, 155, 1426
BstFNI CGCG 2 cut(s) 961, 1014
BstHHI GCGC 2 cut(s) 1029, 1532
BstMAI GTCTC 1 cut(s) 846
BstMCI CGRYCG 1 cut(s) 1386
BstMWI GCNNNNNNNGC 3 cut(s) 767, 776, 1634
BstNSI RCATGY 1 cut(s) 1342
BstPI GGTNACC 1 cut(s) 1313
BstSCI CCNGG 1 cut(s) 439
BstSFI CTRYAG 1 cut(s) 543
BstSLI GKGCMC 2 cut(s) 1236, 1473
BstUI CGCG 2 cut(s) 961, 1014
BstV1I GCAGC 2 cut(s) 474, 1539
BstV2I GAAGAC 1 cut(s) 1119
BstX2I RGATCY 5 cut(s) 322, 373, 547, 646, 902
BstYI RGATCY 5 cut(s) 322, 373, 547, 646, 902
Bsu15I ATCGAT 1 cut(s) 783
Bsu36I CCTNAGG 1 cut(s) 1299
BsuRI GGCC 4 cut(s) 107, 342, 473, 1242
BsuTUI ATCGAT 1 cut(s) 783
BtgI CCRYGG 1 cut(s) 1012
BtgZI GCGATG 1 cut(s) 1384
BtsCI GGATG 3 cut(s) 57, 155, 1426
BtsIMutI CAGTG 2 cut(s) 781, 951
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 1 cut(s) 1473
CfoI GCGC 2 cut(s) 1029, 1532
Cfr42I CCGCGG 1 cut(s) 1015
ClaI ATCGAT 1 cut(s) 783
Csp6I GTAC 7 cut(s) 226, 387, 803, 820, 1180, 1336, 1342
CviAII CATG 3 cut(s) 79, 103, 1339
CviQI GTAC 7 cut(s) 226, 387, 803, 820, 1180, 1336, 1342
DdeI CTNAG 3 cut(s) 344, 377, 1299
DraI TTTAAA 1 cut(s) 634
DrdI GACNNNNNNGTC 1 cut(s) 974
DseDI GACNNNNNNGTC 1 cut(s) 974
Eam1104I CTCTTC 3 cut(s) 22, 825, 1200
EarI CTCTTC 3 cut(s) 22, 825, 1200
Eco130I CCWWGG 3 cut(s) 108, 468, 1237
Eco147I AGGCCT 2 cut(s) 342, 1242
Eco57I CTGAAG 1 cut(s) 849
Eco81I CCTNAGG 1 cut(s) 1299
Eco88I CYCGRG 2 cut(s) 583, 896
Eco91I GGTNACC 1 cut(s) 1313
EcoNI CCTNNNNNAGG 1 cut(s) 1247
EcoO65I GGTNACC 1 cut(s) 1313
EcoRI GAATTC 1 cut(s) 513
EcoT14I CCWWGG 3 cut(s) 108, 468, 1237
EcoT22I ATGCAT 2 cut(s) 155, 367
ErhI CCWWGG 3 cut(s) 108, 468, 1237
FaeI CATG 3 cut(s) 82, 106, 1342
FalI AAGNNNNNCTT 2 cut(s) 1556, 1588
FatI CATG 3 cut(s) 78, 102, 1338
FauNDI CATATG 3 cut(s) 155, 1461, 1592
FblI GTMKAC 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 463, 1528
FokI GGATG 3 cut(s) 44, 162, 1433
Fsp4HI GCNGC 2 cut(s) 463, 1528
FspBI CTAG 1 cut(s) 671
FspI TGCGCA 1 cut(s) 1028
GlaI GCGC 2 cut(s) 1028, 1531
GluI GCNGC 2 cut(s) 463, 1528
HaeIII GGCC 4 cut(s) 107, 342, 473, 1242
HapII CCGG 1 cut(s) 440
HhaI GCGC 2 cut(s) 1029, 1532
Hin1II CATG 3 cut(s) 82, 106, 1342
Hin6I GCGC 2 cut(s) 1027, 1530
HinP1I GCGC 2 cut(s) 1027, 1530
HindIII AAGCTT 1 cut(s) 414
HinfI GANTC 9 cut(s) 84, 191, 233, 480, 609, 1145, 1187, 1276, 1346
HpaII CCGG 1 cut(s) 440
HphI GGTGA 6 cut(s) 208, 371, 761, 1121, 1285, 1325
Hpy166II GTNNAC 5 cut(s) 228, 262, 1182, 1234, 1471
Hpy188III TCNNGA 5 cut(s) 827, 1097, 1132, 1504, 1573
Hpy8I GTNNAC 5 cut(s) 228, 262, 1182, 1234, 1471
Hpy99I CGWCG 1 cut(s) 1043
HpyAV CCTTC 3 cut(s) 403, 493, 1253
HpyCH4III ACNGT 4 cut(s) 52, 334, 354, 971
HpyCH4IV ACGT 6 cut(s) 129, 739, 822, 915, 1080, 1228
HpyCH4V TGCA 6 cut(s) 153, 365, 1058, 1234, 1471, 1596
HpyF10VI GCNNNNNNNGC 3 cut(s) 767, 776, 1634
HpyF3I CTNAG 3 cut(s) 344, 377, 1299
HpySE526I ACGT 6 cut(s) 129, 739, 822, 915, 1080, 1228
Hsp92II CATG 3 cut(s) 82, 106, 1342
HspAI GCGC 2 cut(s) 1027, 1530
KspI CCGCGG 1 cut(s) 1015
LguI GCTCTTC 1 cut(s) 1200
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 2 cut(s) 474, 1539
LweI GCATC 3 cut(s) 140, 352, 1045
MaeI CTAG 1 cut(s) 671
MaeII ACGT 6 cut(s) 129, 739, 822, 915, 1080, 1228
MaeIII GTNAC 4 cut(s) 563, 1273, 1313, 1455
MbiI CCGCTC 1 cut(s) 348
MflI RGATCY 5 cut(s) 322, 373, 547, 646, 902
MhlI GDGCHC 3 cut(s) 737, 1236, 1473
MluCI AATT 9 cut(s) 513, 629, 789, 866, 989, 1006, 1033, 1486, 1578
MlyI GAGTC 3 cut(s) 200, 1270, 1355
MmeI TCCRAC 4 cut(s) 300, 456, 624, 1304
Mph1103I ATGCAT 2 cut(s) 155, 367
MseI TTAA 7 cut(s) 126, 395, 633, 743, 918, 1221, 1514
MslI CAYNNNNRTG 4 cut(s) 962, 1163, 1464, 1476
MspA1I CMGCKG 1 cut(s) 1014
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 441
Mva1269I GAATGC 1 cut(s) 365
MvnI CGCG 2 cut(s) 961, 1014
MwoI GCNNNNNNNGC 3 cut(s) 767, 776, 1634
NciI CCSGG 1 cut(s) 441
NdeI CATATG 3 cut(s) 155, 1461, 1592
NlaIII CATG 3 cut(s) 82, 106, 1342
NlaIV GGNNCC 3 cut(s) 324, 760, 769
NmuCI GTSAC 3 cut(s) 563, 1273, 1313
NsbI TGCGCA 1 cut(s) 1028
NsiI ATGCAT 2 cut(s) 155, 367
NspI RCATGY 1 cut(s) 1342
NspV TTCGAA 1 cut(s) 1063
OliI CACNNNNGTG 1 cut(s) 1476
PceI AGGCCT 2 cut(s) 342, 1242
PciI ACATGT 1 cut(s) 1338
PciSI GCTCTTC 1 cut(s) 1200
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 1 cut(s) 365
PfeI GAWTC 6 cut(s) 84, 233, 480, 609, 1145, 1187
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 2 cut(s) 464, 1529
Ple19I CGATCG 1 cut(s) 1386
PleI GAGTC 3 cut(s) 199, 1270, 1354
PpsI GAGTC 3 cut(s) 199, 1270, 1354
PscI ACATGT 1 cut(s) 1338
PshBI ATTAAT 1 cut(s) 395
PsiI TTATAA 1 cut(s) 66
Psp1406I AACGTT 1 cut(s) 1080
PspEI GGTNACC 1 cut(s) 1313
PspN4I GGNNCC 3 cut(s) 324, 760, 769
PsuI RGATCY 5 cut(s) 322, 373, 547, 646, 902
PvuI CGATCG 1 cut(s) 1386
RsaI GTAC 7 cut(s) 227, 388, 804, 821, 1181, 1337, 1343
RsaNI GTAC 7 cut(s) 226, 387, 803, 820, 1180, 1336, 1342
RseI CAYNNNNRTG 4 cut(s) 962, 1163, 1464, 1476
SacII CCGCGG 1 cut(s) 1015
SapI GCTCTTC 1 cut(s) 1200
SaqAI TTAA 7 cut(s) 126, 395, 633, 743, 918, 1221, 1514
SatI GCNGC 2 cut(s) 463, 1528
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 3 cut(s) 200, 1270, 1355
ScrFI CCNGG 1 cut(s) 441
SduI GDGCHC 3 cut(s) 737, 1236, 1473
SfaNI GCATC 3 cut(s) 140, 352, 1045
SfcI CTRYAG 1 cut(s) 543
Sfr303I CCGCGG 1 cut(s) 1015
SfuI TTCGAA 1 cut(s) 1063
SgrBI CCGCGG 1 cut(s) 1015
SmiMI CAYNNNNRTG 4 cut(s) 962, 1163, 1464, 1476
SmlI CTYRAG 1 cut(s) 1247
SmoI CTYRAG 1 cut(s) 1247
Sse9I AATT 9 cut(s) 513, 629, 789, 866, 989, 1006, 1033, 1486, 1578
SseBI AGGCCT 2 cut(s) 342, 1242
SsiI CCGC 8 cut(s) 41, 278, 348, 756, 794, 1012, 1014, 1628
SspMI CTAG 1 cut(s) 671
StuI AGGCCT 2 cut(s) 342, 1242
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 3 cut(s) 108, 468, 1237
TaaI ACNGT 4 cut(s) 52, 334, 354, 971
TaiI ACGT 6 cut(s) 132, 742, 825, 918, 1083, 1231
TasI AATT 9 cut(s) 513, 629, 789, 866, 989, 1006, 1033, 1486, 1578
TatI WGTACW 2 cut(s) 386, 1335
TfiI GAWTC 6 cut(s) 84, 233, 480, 609, 1145, 1187
Tru1I TTAA 7 cut(s) 126, 395, 633, 743, 918, 1221, 1514
Tru9I TTAA 7 cut(s) 126, 395, 633, 743, 918, 1221, 1514
TscAI CASTG 2 cut(s) 781, 958
TseFI GTSAC 3 cut(s) 563, 1273, 1313
TseI GCWGC 2 cut(s) 462, 1527
Tsp45I GTSAC 3 cut(s) 563, 1273, 1313
TspDTI ATGAA 7 cut(s) 46, 76, 266, 860, 991, 1075, 1437
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 2 cut(s) 781, 958
Van91I CCANNNNNTGG 1 cut(s) 295
VneI GTGCAC 2 cut(s) 1232, 1469
VspI ATTAAT 1 cut(s) 395
XagI CCTNNNNNAGG 1 cut(s) 1247
XapI RAATTY 6 cut(s) 513, 629, 866, 989, 1486, 1578
XceI RCATGY 1 cut(s) 1342
XcmI CCANNNNNNNNNTGG 1 cut(s) 1597
XmiI GTMKAC 1 cut(s) 261
XspI CTAG 1 cut(s) 671
ZrmI AGTACT 1 cut(s) 388
Zsp2I ATGCAT 2 cut(s) 155, 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.