Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0027375

Physical Location & Seq

Genomic Coordinates Forward (+)

20451228 .. 20452569

Gene Structure 1342 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G50240.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 930 bp

ATGAACACGAACACACAAACAGAACAACAGATCATCAGAGAAGAAACTAGAATTGACAAAATTATTAAAAAGCGAAAAAAGAAAATGAGAGCTCAGGTTTTATTATGTCCAACAATAACAGCTTATGGTCGTCTCTATTGTGCGCCACGTAGACTTCACCGCTACAATTCATCATCATCATCATCTCAGTTTCTGAATCTCAATCTCTCTCGCTTCTCCGGTGCTCTCTTCTTCCACATGGAGCAATTCCAGAGTGGAACTGGCTCGAGCGGCAAGAGAGGGATGGTGGAAAATTTGAAGCGGTACGGTGTGATATCGTCGAAAAGAGTAGCTCAAGTTATGGAGGCTTTAGATAGAGGTCTCTTTGTACCAGTTGGTTCTTCAGCTTATGTTGATACTCCTGTACCTATAGGTTATAATGCTACCATTTCTGCTCCGCATATGCATGCTACTTGCTTGCAGCTCTTGGAAGATAAGCTCCACCCCGGGATGCGCGCTTTAGATGTCGGCTCAGGAACCGGGTACTTGACTGGCTGTTTTGCTTTGATGGTTGGAGCTGAGGGACGTGTTGTTGGCGTGGATCATATCCCTGAATTGGTTGATATGTCTATCAAGAATATCGAAAAGAGTGTTGCTGCTTCCTTTCTTAAGAAAGGATCTCTCTCCTTACATGTTGGGGATGGAAGAAAAGGTTGGCAAGAGTTTGCACCGTATGATGCAATACACGTAGGAGCAGCGGCATCAGAAATCCCTCAGCCCCTTTTGGATCAGCTAAAACCCGGTGGACGAATGGTGATTCCACTAGGAACCTATTTTCAAGAGCTCAAGGTAATCGACAAAAACGAGGATGGTTCAATCAAGGTACATACCGAAACTTCAGTCCGGTATGTGCCCCTCACTAGCCGTGTCGAACAGTTAGGAGGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004719 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006479 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008168 GO:0008171 GO:0008213 GO:0008276 GO:0008757 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009845 GO:0009987 GO:0010033 GO:0010340 GO:0016740 GO:0016741 GO:0019538 GO:0030091 GO:0032259 GO:0032501 GO:0032502 GO:0033993 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048856 GO:0050896 GO:0051998 GO:0071704 GO:0090351 GO:0097305 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PCMT

Protein Analysis

309

Amino Acids

33.99

Weight (kDa)

9.42

Isoelectric Point (pI)

35.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PCMT	PF01135	90 - 300	3.7e-82	Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
Methyltransf_31	PF13847	158 - 227	4.2e-07	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0027375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50240 AT5G50240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	417
AccBSI	CCGCTC	1 cut(s)	270
AccI	GTMKAC	1 cut(s)	151
AccII	CGCG	1 cut(s)	495
AciI	CCGC	5 cut(s)	160, 270, 301, 437, 737
AclWI	GGATC	3 cut(s)	588, 664, 774
AcsI	RAATTY	1 cut(s)	292
AcuI	CTGAAG	2 cut(s)	366, 861
AfaI	GTAC	5 cut(s)	305, 369, 405, 524, 864
AfiI	CCNNNNNNNGG	1 cut(s)	595
AflII	CTTAAG	1 cut(s)	647
AflIII	ACRYGT	3 cut(s)	565, 670, 724
AgsI	TTSAA	3 cut(s)	298, 818, 855
AjiI	CACGTC	1 cut(s)	566
AjuI	GAANNNNNNNTTGG	2 cut(s)	676, 708
AluBI	AGCT	9 cut(s)	92, 122, 332, 386, 463, 478, 557, 772, 823
AluI	AGCT	9 cut(s)	92, 122, 332, 386, 463, 478, 557, 772, 823
Alw21I	GWGCWC	3 cut(s)	94, 226, 825
Alw26I	GTCTC	2 cut(s)	137, 365
AlwI	GGATC	3 cut(s)	588, 664, 774
AlwNI	CAGNNNCTG	1 cut(s)	193
Ama87I	CYCGRG	2 cut(s)	265, 485
ApeKI	GCWGC	3 cut(s)	460, 635, 734
ApoI	RAATTY	1 cut(s)	292
ArsI	GACNNNNNNTTYG	2 cut(s)	864, 896
AspLEI	GCGC	3 cut(s)	145, 495, 497
AsuC2I	CCSGG	4 cut(s)	486, 487, 520, 780
AsuHPI	GGTGA	2 cut(s)	149, 805
AvaI	CYCGRG	2 cut(s)	265, 485
BaeGI	GKGCMC	1 cut(s)	894
BaeI	ACNNNNGTAYC	4 cut(s)	387, 387, 420, 420
BanII	GRGCYC	2 cut(s)	94, 825
Bbv12I	GWGCWC	3 cut(s)	94, 226, 825
BbvCI	CCTCAGC	2 cut(s)	558, 753
BbvI	GCAGC	3 cut(s)	472, 622, 746
BccI	CCATC	4 cut(s)	277, 541, 674, 842
BceAI	ACGGC	1 cut(s)	888
BcnI	CCSGG	4 cut(s)	486, 487, 520, 780
BcoDI	GTCTC	2 cut(s)	137, 365
BfaI	CTAG	3 cut(s)	48, 803, 900
BfmI	CTRYAG	1 cut(s)	408
BfrI	CTTAAG	1 cut(s)	647
BisI	GCNGC	5 cut(s)	271, 461, 636, 735, 738
BlsI	GCNGC	5 cut(s)	272, 462, 637, 736, 739
Bme1390I	CCNGG	4 cut(s)	486, 487, 520, 780
BmeT110I	CYCGRG	2 cut(s)	265, 485
BmgBI	CACGTC	1 cut(s)	566
BmiI	GGNNCC	2 cut(s)	517, 808
BmrFI	CCNGG	4 cut(s)	486, 487, 520, 780
BmsI	GCATC	3 cut(s)	480, 706, 749
Bpu10I	CCTNAGC	4 cut(s)	93, 511, 558, 753
BpuEI	CTTGAG	2 cut(s)	318, 809
BpuMI	CCSGG	4 cut(s)	486, 487, 520, 780
BsaAI	YACGTR	2 cut(s)	149, 727
BsaI	GGTCTC	1 cut(s)	365
BsaJI	CCNNGG	2 cut(s)	484, 485
BsaWI	WCCGGW	2 cut(s)	218, 882
Bsc4I	CCNNNNNNNGG	1 cut(s)	595
Bse1I	ACTGG	3 cut(s)	265, 371, 535
BseDI	CCNNGG	2 cut(s)	484, 485
BseGI	GGATG	4 cut(s)	288, 495, 685, 853
BseLI	CCNNNNNNNGG	1 cut(s)	595
BseMII	CTCAG	5 cut(s)	107, 200, 525, 549, 767
BseNI	ACTGG	3 cut(s)	265, 371, 535
BsePI	GCGCGC	1 cut(s)	493
BseSI	GKGCMC	1 cut(s)	894
BseXI	GCAGC	3 cut(s)	472, 622, 746
Bsh1236I	CGCG	1 cut(s)	495
BsiHKAI	GWGCWC	3 cut(s)	94, 226, 825
BsiHKCI	CYCGRG	2 cut(s)	265, 485
BsiSI	CCGG	5 cut(s)	219, 486, 519, 780, 883
BslFI	GGGAC	1 cut(s)	576
BslI	CCNNNNNNNGG	1 cut(s)	595
BsmAI	GTCTC	2 cut(s)	137, 365
BsmBI	CGTCTC	1 cut(s)	137
BsmFI	GGGAC	1 cut(s)	576
Bso31I	GGTCTC	1 cut(s)	365
BsoBI	CYCGRG	2 cut(s)	265, 485
Bsp1286I	GDGCHC	4 cut(s)	94, 226, 825, 894
Bsp143I	GATC	4 cut(s)	30, 580, 656, 766
BspACI	CCGC	5 cut(s)	160, 270, 301, 437, 737
BspCNI	CTCAG	5 cut(s)	106, 199, 524, 550, 766
BspFNI	CGCG	1 cut(s)	495
BspLI	GGNNCC	2 cut(s)	517, 808
BspPI	GGATC	3 cut(s)	588, 664, 774
BspTI	CTTAAG	1 cut(s)	647
BspTNI	GGTCTC	1 cut(s)	365
BsrBI	CCGCTC	1 cut(s)	270
BsrI	ACTGG	3 cut(s)	265, 371, 535
BssECI	CCNNGG	2 cut(s)	484, 485
BssHII	GCGCGC	1 cut(s)	493
BssMI	GATC	4 cut(s)	30, 580, 656, 766
Bst4CI	ACNGT	3 cut(s)	308, 711, 915
Bst6I	CTCTTC	1 cut(s)	233
BstAFI	CTTAAG	1 cut(s)	647
BstBAI	YACGTR	2 cut(s)	149, 727
BstC8I	GCNNGC	3 cut(s)	447, 458, 495
BstDEI	CTNAG	5 cut(s)	93, 186, 511, 558, 753
BstF5I	GGATG	4 cut(s)	288, 495, 685, 853
BstFNI	CGCG	1 cut(s)	495
BstHHI	GCGC	3 cut(s)	145, 495, 497
BstKTI	GATC	4 cut(s)	33, 583, 659, 769
BstMAI	GTCTC	2 cut(s)	137, 365
BstMBI	GATC	4 cut(s)	30, 580, 656, 766
BstMWI	GCNNNNNNNGC	1 cut(s)	270
BstNSI	RCATGY	2 cut(s)	449, 674
BstSCI	CCNGG	4 cut(s)	484, 485, 518, 778
BstSFI	CTRYAG	1 cut(s)	408
BstSLI	GKGCMC	1 cut(s)	894
BstUI	CGCG	1 cut(s)	495
BstV1I	GCAGC	3 cut(s)	472, 622, 746
BstX2I	RGATCY	1 cut(s)	656
BstYI	RGATCY	1 cut(s)	656
BtrI	CACGTC	1 cut(s)	566
BtsCI	GGATG	4 cut(s)	288, 495, 685, 853
Cac8I	GCNNGC	3 cut(s)	447, 458, 495
CaiI	CAGNNNCTG	1 cut(s)	193
CfoI	GCGC	3 cut(s)	145, 495, 497
Cfr9I	CCCGGG	1 cut(s)	485
Csp6I	GTAC	5 cut(s)	304, 368, 404, 523, 863
CviAII	CATG	3 cut(s)	238, 446, 671
CviQI	GTAC	5 cut(s)	304, 368, 404, 523, 863
DdeI	CTNAG	5 cut(s)	93, 186, 511, 558, 753
DpnI	GATC	4 cut(s)	32, 582, 658, 768
DpnII	GATC	4 cut(s)	30, 580, 656, 766
Eam1104I	CTCTTC	1 cut(s)	233
EarI	CTCTTC	1 cut(s)	233
Ecl136II	GAGCTC	2 cut(s)	92, 823
Eco24I	GRGCYC	2 cut(s)	94, 825
Eco31I	GGTCTC	1 cut(s)	365
Eco32I	GATATC	1 cut(s)	315
Eco53kI	GAGCTC	2 cut(s)	92, 823
Eco57I	CTGAAG	2 cut(s)	366, 861
Eco88I	CYCGRG	2 cut(s)	265, 485
EcoICRI	GAGCTC	2 cut(s)	92, 823
EcoRV	GATATC	1 cut(s)	315
EcoT22I	ATGCAT	1 cut(s)	447
EcoT38I	GRGCYC	2 cut(s)	94, 825
Esp3I	CGTCTC	1 cut(s)	137
FaeI	CATG	3 cut(s)	241, 449, 674
FaqI	GGGAC	1 cut(s)	576
FatI	CATG	3 cut(s)	237, 445, 670
FauNDI	CATATG	1 cut(s)	441
FblI	GTMKAC	1 cut(s)	151
Fnu4HI	GCNGC	5 cut(s)	271, 461, 636, 735, 738
FokI	GGATG	4 cut(s)	295, 502, 692, 860
FriOI	GRGCYC	2 cut(s)	94, 825
Fsp4HI	GCNGC	5 cut(s)	271, 461, 636, 735, 738
FspBI	CTAG	3 cut(s)	48, 803, 900
GlaI	GCGC	3 cut(s)	144, 494, 496
GluI	GCNGC	5 cut(s)	271, 461, 636, 735, 738
HapII	CCGG	5 cut(s)	219, 486, 519, 780, 883
HhaI	GCGC	3 cut(s)	145, 495, 497
Hin1II	CATG	3 cut(s)	241, 449, 674
Hin6I	GCGC	3 cut(s)	143, 493, 495
HinP1I	GCGC	3 cut(s)	143, 493, 495
HinfI	GANTC	2 cut(s)	196, 796
HpaII	CCGG	5 cut(s)	219, 486, 519, 780, 883
HphI	GGTGA	2 cut(s)	149, 805
Hpy166II	GTNNAC	2 cut(s)	152, 785
Hpy188I	TCNGA	3 cut(s)	38, 195, 745
Hpy188III	TCNNGA	4 cut(s)	250, 513, 613, 818
Hpy8I	GTNNAC	2 cut(s)	152, 785
Hpy99I	CGWCG	1 cut(s)	322
HpyCH4III	ACNGT	3 cut(s)	308, 711, 915
HpyCH4IV	ACGT	3 cut(s)	148, 565, 726
HpyCH4V	TGCA	4 cut(s)	445, 460, 707, 719
HpyF10VI	GCNNNNNNNGC	1 cut(s)	270
HpyF3I	CTNAG	5 cut(s)	93, 186, 511, 558, 753
HpySE526I	ACGT	3 cut(s)	148, 565, 726
Hsp92II	CATG	3 cut(s)	241, 449, 674
HspAI	GCGC	3 cut(s)	143, 493, 495
Kzo9I	GATC	4 cut(s)	30, 580, 656, 766
LmnI	GCTCC	5 cut(s)	241, 439, 483, 554, 731
Lsp1109I	GCAGC	3 cut(s)	472, 622, 746
LweI	GCATC	3 cut(s)	480, 706, 749
MaeI	CTAG	3 cut(s)	48, 803, 900
MaeII	ACGT	3 cut(s)	148, 565, 726
MalI	GATC	4 cut(s)	32, 582, 658, 768
MbiI	CCGCTC	1 cut(s)	270
MboI	GATC	4 cut(s)	30, 580, 656, 766
MboII	GAAGA	6 cut(s)	53, 220, 223, 372, 482, 696
MflI	RGATCY	1 cut(s)	656
MhlI	GDGCHC	4 cut(s)	94, 226, 825, 894
MluCI	AATT	6 cut(s)	51, 60, 166, 245, 292, 593
MmeI	TCCRAC	2 cut(s)	134, 532
MnlI	CCTC	8 cut(s)	272, 337, 350, 553, 762, 838, 905, 914
Mph1103I	ATGCAT	1 cut(s)	447
MseI	TTAA	2 cut(s)	66, 648
MslI	CAYNNNNRTG	1 cut(s)	444
MspA1I	CMGCKG	1 cut(s)	737
MspCI	CTTAAG	1 cut(s)	647
MspI	CCGG	5 cut(s)	219, 486, 519, 780, 883
MspR9I	CCNGG	4 cut(s)	486, 487, 520, 780
MvnI	CGCG	1 cut(s)	495
MwoI	GCNNNNNNNGC	1 cut(s)	270
NciI	CCSGG	4 cut(s)	486, 487, 520, 780
NdeI	CATATG	1 cut(s)	441
NdeII	GATC	4 cut(s)	30, 580, 656, 766
NlaIII	CATG	3 cut(s)	241, 449, 674
NlaIV	GGNNCC	2 cut(s)	517, 808
NsiI	ATGCAT	1 cut(s)	447
NspI	RCATGY	2 cut(s)	449, 674
PaeI	GCATGC	1 cut(s)	449
PaeR7I	CTCGAG	1 cut(s)	265
PauI	GCGCGC	1 cut(s)	493
PciI	ACATGT	1 cut(s)	670
PcsI	WCGNNNNNNNCGW	1 cut(s)	840
PfeI	GAWTC	2 cut(s)	196, 796
PkrI	GCNGC	5 cut(s)	272, 462, 637, 736, 739
Ppu21I	YACGTR	2 cut(s)	149, 727
PscI	ACATGT	1 cut(s)	670
PsiI	TTATAA	1 cut(s)	417
Psp124BI	GAGCTC	2 cut(s)	94, 825
PspN4I	GGNNCC	2 cut(s)	517, 808
PspXI	VCTCGAGB	1 cut(s)	265
PstNI	CAGNNNCTG	1 cut(s)	193
PsuI	RGATCY	1 cut(s)	656
PteI	GCGCGC	1 cut(s)	493
RsaI	GTAC	5 cut(s)	305, 369, 405, 524, 864
RsaNI	GTAC	5 cut(s)	304, 368, 404, 523, 863
RseI	CAYNNNNRTG	1 cut(s)	444
SacI	GAGCTC	2 cut(s)	94, 825
SaqAI	TTAA	2 cut(s)	66, 648
SatI	GCNGC	5 cut(s)	271, 461, 636, 735, 738
Sau3AI	GATC	4 cut(s)	30, 580, 656, 766
ScrFI	CCNGG	4 cut(s)	486, 487, 520, 780
SduI	GDGCHC	4 cut(s)	94, 226, 825, 894
SfaNI	GCATC	3 cut(s)	480, 706, 749
SfcI	CTRYAG	1 cut(s)	408
Sfr274I	CTCGAG	1 cut(s)	265
SlaI	CTCGAG	1 cut(s)	265
SmaI	CCCGGG	1 cut(s)	487
SmiMI	CAYNNNNRTG	1 cut(s)	444
SmlI	CTYRAG	4 cut(s)	265, 333, 647, 824
SmoI	CTYRAG	4 cut(s)	265, 333, 647, 824
SphI	GCATGC	1 cut(s)	449
Sse9I	AATT	6 cut(s)	51, 60, 166, 245, 292, 593
SsiI	CCGC	5 cut(s)	160, 270, 301, 437, 737
SspMI	CTAG	3 cut(s)	48, 803, 900
SstI	GAGCTC	2 cut(s)	94, 825
StyD4I	CCNGG	4 cut(s)	484, 485, 518, 778
TaaI	ACNGT	3 cut(s)	308, 711, 915
TaiI	ACGT	3 cut(s)	151, 568, 729
TaqI	TCGA	5 cut(s)	266, 320, 621, 834, 909
TasI	AATT	6 cut(s)	51, 60, 166, 245, 292, 593
TauI	GCSGC	2 cut(s)	273, 740
TfiI	GAWTC	2 cut(s)	196, 796
Tru1I	TTAA	2 cut(s)	66, 648
Tru9I	TTAA	2 cut(s)	66, 648
TseI	GCWGC	3 cut(s)	460, 635, 734
TspDTI	ATGAA	2 cut(s)	17, 159
TspMI	CCCGGG	1 cut(s)	485
Vha464I	CTTAAG	1 cut(s)	647
XapI	RAATTY	1 cut(s)	292
XceI	RCATGY	2 cut(s)	449, 674
XcmI	CCANNNNNNNNNTGG	1 cut(s)	257
XhoI	CTCGAG	1 cut(s)	265
XmaI	CCCGGG	1 cut(s)	485
XmiI	GTMKAC	1 cut(s)	151
XspI	CTAG	3 cut(s)	48, 803, 900
Zsp2I	ATGCAT	1 cut(s)	447

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.