U-box domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 179 |
| AatII | GACGTC | 1 cut(s) | 2324 |
| AbsI | CCTCGAGG | 1 cut(s) | 478 |
| Acc36I | ACCTGC | 1 cut(s) | 2021 |
| AccB1I | GGYRCC | 1 cut(s) | 1491 |
| AccBSI | CCGCTC | 1 cut(s) | 352 |
| AccII | CGCG | 1 cut(s) | 1701 |
| AciI | CCGC | 9 cut(s) | 77, 278, 352, 1410, 1496, 1624, 1699, 2187, 2261 |
| AclI | AACGTT | 2 cut(s) | 1067, 1841 |
| AclWI | GGATC | 2 cut(s) | 1771, 1850 |
| AcoI | YGGCCR | 1 cut(s) | 2039 |
| AcsI | RAATTY | 2 cut(s) | 1002, 1805 |
| AcuI | CTGAAG | 1 cut(s) | 1362 |
| AcvI | CACGTG | 1 cut(s) | 1305 |
| AcyI | GRCGYC | 1 cut(s) | 2321 |
| AdeI | CACNNNGTG | 1 cut(s) | 863 |
| AfaI | GTAC | 4 cut(s) | 1553, 1817, 2086, 2273 |
| AfeI | AGCGCT | 1 cut(s) | 1656 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 480, 1501, 1532 |
| AflII | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| AflIII | ACRYGT | 2 cut(s) | 579, 1899 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1795 |
| AhlI | ACTAGT | 1 cut(s) | 773 |
| AjiI | CACGTC | 2 cut(s) | 582, 1900 |
| AjnI | CCWGG | 1 cut(s) | 984 |
| AleI | CACNNNNGTG | 1 cut(s) | 2080 |
| Alw21I | GWGCWC | 4 cut(s) | 533, 1128, 1470, 2094 |
| Alw26I | GTCTC | 4 cut(s) | 253, 302, 550, 2328 |
| Alw44I | GTGCAC | 1 cut(s) | 529 |
| AlwI | GGATC | 2 cut(s) | 1771, 1850 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 515 |
| Ama87I | CYCGRG | 1 cut(s) | 478 |
| Aor51HI | AGCGCT | 1 cut(s) | 1656 |
| AoxI | GGCC | 2 cut(s) | 2039, 2108 |
| ApaLI | GTGCAC | 1 cut(s) | 529 |
| ApoI | RAATTY | 2 cut(s) | 1002, 1805 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 566, 598 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 321 |
| AspLEI | GCGC | 4 cut(s) | 154, 1538, 1657, 2194 |
| AspS9I | GGNCC | 1 cut(s) | 31 |
| AsuII | TTCGAA | 1 cut(s) | 1007 |
| AvaI | CYCGRG | 1 cut(s) | 478 |
| AvaII | GGWCC | 1 cut(s) | 31 |
| BaeGI | GKGCMC | 2 cut(s) | 52, 533 |
| BalI | TGGCCA | 1 cut(s) | 2041 |
| BanI | GGYRCC | 1 cut(s) | 1491 |
| BanII | GRGCYC | 2 cut(s) | 1470, 2094 |
| BbrPI | CACGTG | 1 cut(s) | 1305 |
| BbsI | GAAGAC | 1 cut(s) | 801 |
| Bbv12I | GWGCWC | 4 cut(s) | 533, 1128, 1470, 2094 |
| BccI | CCATC | 4 cut(s) | 213, 2261, 2299, 2396 |
| BceAI | ACGGC | 1 cut(s) | 2326 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2160, 2194 |
| BciT130I | CCWGG | 1 cut(s) | 986 |
| BclI | TGATCA | 3 cut(s) | 435, 1528, 1917 |
| BcoDI | GTCTC | 4 cut(s) | 253, 302, 550, 2328 |
| BcuI | ACTAGT | 1 cut(s) | 773 |
| BfaI | CTAG | 6 cut(s) | 266, 774, 927, 1514, 1541, 2055 |
| BfmI | CTRYAG | 3 cut(s) | 228, 819, 1925 |
| BfoI | RGCGCY | 3 cut(s) | 155, 1539, 1658 |
| BfrI | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| BfuAI | ACCTGC | 1 cut(s) | 2021 |
| BlpI | GCTNAGC | 1 cut(s) | 1020 |
| Bme1390I | CCNGG | 1 cut(s) | 986 |
| Bme18I | GGWCC | 1 cut(s) | 31 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1795 |
| BmeT110I | CYCGRG | 1 cut(s) | 478 |
| BmgBI | CACGTC | 2 cut(s) | 582, 1900 |
| BmgT120I | GGNCC | 1 cut(s) | 31 |
| BmiI | GGNNCC | 5 cut(s) | 33, 81, 1278, 1493, 2250 |
| BmrFI | CCNGG | 1 cut(s) | 986 |
| BmsI | GCATC | 6 cut(s) | 291, 521, 805, 1063, 1405, 1658 |
| BpiI | GAAGAC | 1 cut(s) | 801 |
| BpmI | CTGGAG | 3 cut(s) | 622, 1142, 2063 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1281 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1020 |
| Bpu14I | TTCGAA | 1 cut(s) | 1007 |
| BpuEI | CTTGAG | 3 cut(s) | 450, 1041, 1498 |
| Bsa29I | ATCGAT | 1 cut(s) | 1854 |
| BsaAI | YACGTR | 2 cut(s) | 860, 1305 |
| BsaBI | GATNNNNATC | 1 cut(s) | 200 |
| BsaHI | GRCGYC | 1 cut(s) | 2321 |
| BsaJI | CCNNGG | 5 cut(s) | 258, 961, 1613, 1833, 1980 |
| BsaWI | WCCGGW | 4 cut(s) | 1874, 2283, 2316, 2326 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 480, 1501, 1532 |
| Bse1I | ACTGG | 4 cut(s) | 1942, 2042, 2080, 2135 |
| Bse8I | GATNNNNATC | 1 cut(s) | 200 |
| BseBI | CCWGG | 1 cut(s) | 986 |
| BseCI | ATCGAT | 1 cut(s) | 1854 |
| BseDI | CCNNGG | 5 cut(s) | 258, 961, 1613, 1833, 1980 |
| BseGI | GGATG | 8 cut(s) | 201, 205, 820, 1078, 1381, 1692, 2242, 2310 |
| BseJI | GATNNNNATC | 1 cut(s) | 200 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 480, 1501, 1532 |
| BseMII | CTCAG | 5 cut(s) | 681, 774, 893, 1011, 1206 |
| BseNI | ACTGG | 4 cut(s) | 1942, 2042, 2080, 2135 |
| BseRI | GAGGAG | 3 cut(s) | 466, 570, 2312 |
| BseSI | GKGCMC | 2 cut(s) | 52, 533 |
| BseYI | CCCAGC | 1 cut(s) | 51 |
| BsgI | GTGCAG | 1 cut(s) | 1182 |
| Bsh1236I | CGCG | 1 cut(s) | 1701 |
| BshFI | GGCC | 2 cut(s) | 2041, 2110 |
| BshNI | GGYRCC | 1 cut(s) | 1491 |
| BshVI | ATCGAT | 1 cut(s) | 1854 |
| BsiHKAI | GWGCWC | 4 cut(s) | 533, 1128, 1470, 2094 |
| BsiHKCI | CYCGRG | 1 cut(s) | 478 |
| BsiSI | CCGG | 4 cut(s) | 1875, 2284, 2317, 2327 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 480, 1501, 1532 |
| BsmAI | GTCTC | 4 cut(s) | 253, 302, 550, 2328 |
| BsmBI | CGTCTC | 1 cut(s) | 2328 |
| BsmI | GAATGC | 3 cut(s) | 688, 729, 1426 |
| BsnI | GGCC | 2 cut(s) | 2041, 2110 |
| BsoBI | CYCGRG | 1 cut(s) | 478 |
| Bsp119I | TTCGAA | 1 cut(s) | 1007 |
| Bsp1286I | GDGCHC | 5 cut(s) | 52, 533, 1128, 1470, 2094 |
| Bsp1407I | TGTACA | 2 cut(s) | 1815, 2084 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1020 |
| Bsp19I | CCATGG | 1 cut(s) | 1980 |
| BspACI | CCGC | 9 cut(s) | 77, 278, 352, 1410, 1496, 1624, 1699, 2187, 2261 |
| BspANI | GGCC | 2 cut(s) | 2041, 2110 |
| BspCNI | CTCAG | 5 cut(s) | 680, 775, 892, 1012, 1207 |
| BspDI | ATCGAT | 1 cut(s) | 1854 |
| BspFNI | CGCG | 1 cut(s) | 1701 |
| BspHI | TCATGA | 1 cut(s) | 1036 |
| BspLI | GGNNCC | 5 cut(s) | 33, 81, 1278, 1493, 2250 |
| BspMAI | CTGCAG | 2 cut(s) | 823, 1929 |
| BspMI | ACCTGC | 1 cut(s) | 2021 |
| BspPI | GGATC | 2 cut(s) | 1771, 1850 |
| BspQI | GCTCTTC | 1 cut(s) | 1101 |
| BspT104I | TTCGAA | 1 cut(s) | 1007 |
| BspT107I | GGYRCC | 1 cut(s) | 1491 |
| BspTI | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| BsrBI | CCGCTC | 1 cut(s) | 352 |
| BsrGI | TGTACA | 2 cut(s) | 1815, 2084 |
| BsrI | ACTGG | 4 cut(s) | 1942, 2042, 2080, 2135 |
| BssECI | CCNNGG | 5 cut(s) | 258, 961, 1613, 1833, 1980 |
| BssNI | GRCGYC | 1 cut(s) | 2321 |
| BssT1I | CCWWGG | 2 cut(s) | 961, 1980 |
| Bst2UI | CCWGG | 1 cut(s) | 986 |
| Bst4CI | ACNGT | 5 cut(s) | 100, 535, 1565, 1584, 1969 |
| Bst6I | CTCTTC | 5 cut(s) | 327, 1101, 1169, 1179, 1201 |
| BstACI | GRCGYC | 1 cut(s) | 2321 |
| BstAFI | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| BstAUI | TGTACA | 2 cut(s) | 1815, 2084 |
| BstBAI | YACGTR | 2 cut(s) | 860, 1305 |
| BstBI | TTCGAA | 1 cut(s) | 1007 |
| BstC8I | GCNNGC | 4 cut(s) | 48, 350, 832, 1713 |
| BstDEI | CTNAG | 8 cut(s) | 60, 667, 783, 879, 1020, 1025, 1215, 1281 |
| BstDSI | CCRYGG | 3 cut(s) | 1613, 1833, 1980 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 478 |
| BstF5I | GGATG | 8 cut(s) | 201, 205, 820, 1078, 1381, 1692, 2242, 2310 |
| BstFNI | CGCG | 1 cut(s) | 1701 |
| BstH2I | RGCGCY | 3 cut(s) | 155, 1539, 1658 |
| BstHHI | GCGC | 4 cut(s) | 154, 1538, 1657, 2194 |
| BstMAI | GTCTC | 4 cut(s) | 253, 302, 550, 2328 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 47, 52, 74, 129, 827, 1093, 1123, 2072, 2168 |
| BstNI | CCWGG | 1 cut(s) | 986 |
| BstNSI | RCATGY | 1 cut(s) | 1524 |
| BstSCI | CCNGG | 1 cut(s) | 984 |
| BstSFI | CTRYAG | 3 cut(s) | 228, 819, 1925 |
| BstSLI | GKGCMC | 2 cut(s) | 52, 533 |
| BstUI | CGCG | 1 cut(s) | 1701 |
| BstV2I | GAAGAC | 1 cut(s) | 801 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1614 |
| Bsu15I | ATCGAT | 1 cut(s) | 1854 |
| BsuRI | GGCC | 2 cut(s) | 2041, 2110 |
| BsuTUI | ATCGAT | 1 cut(s) | 1854 |
| BtgI | CCRYGG | 3 cut(s) | 1613, 1833, 1980 |
| BtrI | CACGTC | 2 cut(s) | 582, 1900 |
| BtsCI | GGATG | 8 cut(s) | 201, 205, 820, 1078, 1381, 1692, 2242, 2310 |
| BtsI | GCAGTG | 2 cut(s) | 408, 1056 |
| BtsIMutI | CAGTG | 5 cut(s) | 408, 1056, 1935, 1955, 2087 |
| BveI | ACCTGC | 1 cut(s) | 2021 |
| Cac8I | GCNNGC | 4 cut(s) | 48, 350, 832, 1713 |
| CaiI | CAGNNNCTG | 1 cut(s) | 515 |
| CciI | TCATGA | 1 cut(s) | 1036 |
| CfoI | GCGC | 4 cut(s) | 154, 1538, 1657, 2194 |
| Cfr13I | GGNCC | 1 cut(s) | 31 |
| ClaI | ATCGAT | 1 cut(s) | 1854 |
| CseI | GACGC | 3 cut(s) | 465, 489, 917 |
| Csp6I | GTAC | 4 cut(s) | 1552, 1816, 2085, 2272 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1815, 1850 |
| CviAII | CATG | 8 cut(s) | 922, 1037, 1232, 1521, 1532, 1555, 1981, 2253 |
| CviQI | GTAC | 4 cut(s) | 1552, 1816, 2085, 2272 |
| DdeI | CTNAG | 8 cut(s) | 60, 667, 783, 879, 1020, 1025, 1215, 1281 |
| DraI | TTTAAA | 1 cut(s) | 1350 |
| DraIII | CACNNNGTG | 1 cut(s) | 863 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 179 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1795 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 179 |
| EaeI | YGGCCR | 1 cut(s) | 2039 |
| Eam1104I | CTCTTC | 5 cut(s) | 327, 1101, 1169, 1179, 1201 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1795 |
| EarI | CTCTTC | 5 cut(s) | 327, 1101, 1169, 1179, 1201 |
| Ecl136II | GAGCTC | 2 cut(s) | 1468, 2092 |
| Eco130I | CCWWGG | 2 cut(s) | 961, 1980 |
| Eco147I | AGGCCT | 1 cut(s) | 2110 |
| Eco24I | GRGCYC | 2 cut(s) | 1470, 2094 |
| Eco32I | GATATC | 2 cut(s) | 507, 1884 |
| Eco47I | GGWCC | 1 cut(s) | 31 |
| Eco47III | AGCGCT | 1 cut(s) | 1656 |
| Eco53kI | GAGCTC | 2 cut(s) | 1468, 2092 |
| Eco57I | CTGAAG | 1 cut(s) | 1362 |
| Eco72I | CACGTG | 1 cut(s) | 1305 |
| Eco88I | CYCGRG | 1 cut(s) | 478 |
| EcoICRI | GAGCTC | 2 cut(s) | 1468, 2092 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 478 |
| EcoRII | CCWGG | 1 cut(s) | 984 |
| EcoRV | GATATC | 2 cut(s) | 507, 1884 |
| EcoT14I | CCWWGG | 2 cut(s) | 961, 1980 |
| EcoT38I | GRGCYC | 2 cut(s) | 1470, 2094 |
| ErhI | CCWWGG | 2 cut(s) | 961, 1980 |
| Esp3I | CGTCTC | 1 cut(s) | 2328 |
| FaeI | CATG | 8 cut(s) | 925, 1040, 1235, 1524, 1535, 1558, 1984, 2256 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 306, 338, 1621, 1653 |
| FatI | CATG | 8 cut(s) | 921, 1036, 1231, 1520, 1531, 1554, 1980, 2252 |
| FauI | CCCGC | 1 cut(s) | 1503 |
| FbaI | TGATCA | 3 cut(s) | 435, 1528, 1917 |
| FokI | GGATG | 8 cut(s) | 188, 192, 827, 1085, 1388, 1679, 2249, 2317 |
| FriOI | GRGCYC | 2 cut(s) | 1470, 2094 |
| FspBI | CTAG | 6 cut(s) | 266, 774, 927, 1514, 1541, 2055 |
| GlaI | GCGC | 4 cut(s) | 153, 1537, 1656, 2193 |
| GsaI | CCCAGC | 1 cut(s) | 55 |
| GsuI | CTGGAG | 3 cut(s) | 622, 1142, 2063 |
| HaeII | RGCGCY | 3 cut(s) | 155, 1539, 1658 |
| HaeIII | GGCC | 2 cut(s) | 2041, 2110 |
| HapII | CCGG | 4 cut(s) | 1875, 2284, 2317, 2327 |
| HgaI | GACGC | 3 cut(s) | 465, 489, 917 |
| HhaI | GCGC | 4 cut(s) | 154, 1538, 1657, 2194 |
| Hin1I | GRCGYC | 1 cut(s) | 2321 |
| Hin1II | CATG | 8 cut(s) | 925, 1040, 1235, 1524, 1535, 1558, 1984, 2256 |
| Hin6I | GCGC | 4 cut(s) | 152, 1536, 1655, 2192 |
| HinP1I | GCGC | 4 cut(s) | 152, 1536, 1655, 2192 |
| HincII | GTYRAC | 5 cut(s) | 1594, 1604, 1800, 1943, 2201 |
| HindII | GTYRAC | 5 cut(s) | 1594, 1604, 1800, 1943, 2201 |
| HindIII | AAGCTT | 2 cut(s) | 1253, 2146 |
| HpaII | CCGG | 4 cut(s) | 1875, 2284, 2317, 2327 |
| Hpy99I | CGWCG | 3 cut(s) | 505, 2000, 2396 |
| HpyAV | CCTTC | 6 cut(s) | 93, 1129, 1173, 1240, 1872, 2239 |
| HpyCH4III | ACNGT | 5 cut(s) | 100, 535, 1565, 1584, 1969 |
| HpyCH4IV | ACGT | 8 cut(s) | 581, 859, 1067, 1304, 1841, 1899, 2321, 2352 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 47, 52, 74, 129, 827, 1093, 1123, 2072, 2168 |
| HpyF3I | CTNAG | 8 cut(s) | 60, 667, 783, 879, 1020, 1025, 1215, 1281 |
| HpySE526I | ACGT | 8 cut(s) | 581, 859, 1067, 1304, 1841, 1899, 2321, 2352 |
| Hsp92I | GRCGYC | 1 cut(s) | 2321 |
| Hsp92II | CATG | 8 cut(s) | 925, 1040, 1235, 1524, 1535, 1558, 1984, 2256 |
| HspAI | GCGC | 4 cut(s) | 152, 1536, 1655, 2192 |
| Ksp22I | TGATCA | 3 cut(s) | 435, 1528, 1917 |
| LguI | GCTCTTC | 1 cut(s) | 1101 |
| LmnI | GCTCC | 9 cut(s) | 85, 120, 659, 1123, 1282, 1364, 1517, 2097, 2248 |
| LweI | GCATC | 6 cut(s) | 291, 521, 805, 1063, 1405, 1658 |
| MaeI | CTAG | 6 cut(s) | 266, 774, 927, 1514, 1541, 2055 |
| MaeII | ACGT | 8 cut(s) | 581, 859, 1067, 1304, 1841, 1899, 2321, 2352 |
| MaeIII | GTNAC | 5 cut(s) | 181, 952, 2033, 2329, 2343 |
| MbiI | CCGCTC | 1 cut(s) | 352 |
| MhlI | GDGCHC | 5 cut(s) | 52, 533, 1128, 1470, 2094 |
| MlsI | TGGCCA | 1 cut(s) | 2041 |
| MluCI | AATT | 8 cut(s) | 141, 484, 1002, 1314, 1454, 1805, 2060, 2335 |
| MluNI | TGGCCA | 1 cut(s) | 2041 |
| MlyI | GAGTC | 2 cut(s) | 567, 794 |
| MmeI | TCCRAC | 1 cut(s) | 1050 |
| Mox20I | TGGCCA | 1 cut(s) | 2041 |
| MroXI | GAANNNNTTC | 1 cut(s) | 321 |
| MscI | TGGCCA | 1 cut(s) | 2041 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1035, 2080 |
| Msp20I | TGGCCA | 1 cut(s) | 2041 |
| MspA1I | CMGCKG | 3 cut(s) | 642, 1412, 2189 |
| MspCI | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| MspI | CCGG | 4 cut(s) | 1875, 2284, 2317, 2327 |
| MspR9I | CCNGG | 1 cut(s) | 986 |
| Mva1269I | GAATGC | 3 cut(s) | 688, 729, 1426 |
| MvaI | CCWGG | 1 cut(s) | 986 |
| MvnI | CGCG | 1 cut(s) | 1701 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 47, 52, 74, 129, 827, 1093, 1123, 2072, 2168 |
| NcoI | CCATGG | 1 cut(s) | 1980 |
| NlaIII | CATG | 8 cut(s) | 925, 1040, 1235, 1524, 1535, 1558, 1984, 2256 |
| NlaIV | GGNNCC | 5 cut(s) | 33, 81, 1278, 1493, 2250 |
| NmuCI | GTSAC | 1 cut(s) | 181 |
| NspI | RCATGY | 1 cut(s) | 1524 |
| NspV | TTCGAA | 1 cut(s) | 1007 |
| OliI | CACNNNNGTG | 1 cut(s) | 2080 |
| PaeR7I | CTCGAG | 1 cut(s) | 478 |
| PagI | TCATGA | 1 cut(s) | 1036 |
| PceI | AGGCCT | 1 cut(s) | 2110 |
| PciSI | GCTCTTC | 1 cut(s) | 1101 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2388 |
| PctI | GAATGC | 3 cut(s) | 688, 729, 1426 |
| PdmI | GAANNNNTTC | 1 cut(s) | 321 |
| PfeI | GAWTC | 7 cut(s) | 196, 386, 614, 874, 1632, 2014, 2128 |
| PleI | GAGTC | 2 cut(s) | 566, 793 |
| PmaCI | CACGTG | 1 cut(s) | 1305 |
| PmlI | CACGTG | 1 cut(s) | 1305 |
| PpsI | GAGTC | 2 cut(s) | 566, 793 |
| Ppu21I | YACGTR | 2 cut(s) | 860, 1305 |
| Psp124BI | GAGCTC | 2 cut(s) | 1470, 2094 |
| Psp1406I | AACGTT | 2 cut(s) | 1067, 1841 |
| Psp6I | CCWGG | 1 cut(s) | 984 |
| PspCI | CACGTG | 1 cut(s) | 1305 |
| PspFI | CCCAGC | 1 cut(s) | 51 |
| PspGI | CCWGG | 1 cut(s) | 984 |
| PspN4I | GGNNCC | 5 cut(s) | 33, 81, 1278, 1493, 2250 |
| PspPI | GGNCC | 1 cut(s) | 31 |
| PspXI | VCTCGAGB | 1 cut(s) | 478 |
| PstI | CTGCAG | 2 cut(s) | 823, 1929 |
| PstNI | CAGNNNCTG | 1 cut(s) | 515 |
| PvuII | CAGCTG | 1 cut(s) | 642 |
| RsaI | GTAC | 4 cut(s) | 1553, 1817, 2086, 2273 |
| RsaNI | GTAC | 4 cut(s) | 1552, 1816, 2085, 2272 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1035, 2080 |
| SacI | GAGCTC | 2 cut(s) | 1470, 2094 |
| SapI | GCTCTTC | 1 cut(s) | 1101 |
| Sau96I | GGNCC | 1 cut(s) | 31 |
| SchI | GAGTC | 2 cut(s) | 567, 794 |
| ScrFI | CCNGG | 1 cut(s) | 986 |
| SduI | GDGCHC | 5 cut(s) | 52, 533, 1128, 1470, 2094 |
| SfaNI | GCATC | 6 cut(s) | 291, 521, 805, 1063, 1405, 1658 |
| SfcI | CTRYAG | 3 cut(s) | 228, 819, 1925 |
| Sfr274I | CTCGAG | 1 cut(s) | 478 |
| SfuI | TTCGAA | 1 cut(s) | 1007 |
| SinI | GGWCC | 1 cut(s) | 31 |
| SlaI | CTCGAG | 1 cut(s) | 478 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1035, 2080 |
| SmlI | CTYRAG | 7 cut(s) | 465, 478, 1056, 1097, 1477, 1706, 2231 |
| SmoI | CTYRAG | 7 cut(s) | 465, 478, 1056, 1097, 1477, 1706, 2231 |
| SpeI | ACTAGT | 1 cut(s) | 773 |
| Sse9I | AATT | 8 cut(s) | 141, 484, 1002, 1314, 1454, 1805, 2060, 2335 |
| SseBI | AGGCCT | 1 cut(s) | 2110 |
| SsiI | CCGC | 9 cut(s) | 77, 278, 352, 1410, 1496, 1624, 1699, 2187, 2261 |
| SspMI | CTAG | 6 cut(s) | 266, 774, 927, 1514, 1541, 2055 |
| SstI | GAGCTC | 2 cut(s) | 1470, 2094 |
| StuI | AGGCCT | 1 cut(s) | 2110 |
| StyD4I | CCNGG | 1 cut(s) | 984 |
| StyI | CCWWGG | 2 cut(s) | 961, 1980 |
| TaaI | ACNGT | 5 cut(s) | 100, 535, 1565, 1584, 1969 |
| TaiI | ACGT | 8 cut(s) | 584, 862, 1070, 1307, 1844, 1902, 2324, 2355 |
| TaqI | TCGA | 9 cut(s) | 479, 503, 680, 1007, 1458, 1470, 1854, 2046, 2394 |
| TasI | AATT | 8 cut(s) | 141, 484, 1002, 1314, 1454, 1805, 2060, 2335 |
| TatI | WGTACW | 3 cut(s) | 1551, 1815, 2084 |
| TauI | GCSGC | 4 cut(s) | 80, 281, 1412, 2264 |
| TfiI | GAWTC | 7 cut(s) | 196, 386, 614, 874, 1632, 2014, 2128 |
| TscAI | CASTG | 5 cut(s) | 408, 1056, 1942, 1962, 2087 |
| TseFI | GTSAC | 1 cut(s) | 181 |
| Tsp45I | GTSAC | 1 cut(s) | 181 |
| TspDTI | ATGAA | 8 cut(s) | 807, 1014, 1053, 1235, 1248, 1320, 2037, 2258 |
| TspGWI | ACGGA | 4 cut(s) | 567, 605, 957, 2084 |
| TspRI | CASTG | 5 cut(s) | 408, 1056, 1942, 1962, 2087 |
| Vha464I | CTTAAG | 3 cut(s) | 1097, 1706, 2231 |
| VneI | GTGCAC | 1 cut(s) | 529 |
| VpaK11BI | GGWCC | 1 cut(s) | 31 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 478 |
| XapI | RAATTY | 2 cut(s) | 1002, 1805 |
| XceI | RCATGY | 1 cut(s) | 1524 |
| XhoI | CTCGAG | 1 cut(s) | 478 |
| XmnI | GAANNNNTTC | 1 cut(s) | 321 |
| XspI | CTAG | 6 cut(s) | 266, 774, 927, 1514, 1541, 2055 |
| ZraI | GACGTC | 1 cut(s) | 2322 |