AT5G60120

AP2-like ethylene-responsive transcription factor TOE2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0020264
Physical Location & Seq
Forward (+)
24207689 .. 24211743
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60120.3

Sequence Viewer

Length: 1458 bp
ATGCTGGATCTCAATCTCGACGTCGACTCGACCGAGTCTACTCAGAACGAACGAGATTCGATTACTGTTAAAGGGGTTTCTTTGAATCAGATGGATGAATCGGTGACGTCGAACTCTTCTGTTGTTAATGCTGAAGCTTCTAGCTGTATAGATGGTGAAGATGAGTTGTGCTCTACACGAACGGTCAAGTTTCAATTTGAGATACTGAAAGGAGGAGGAGAAGAAGAAGAAGAAGATGATGATGAAAGAAGTGCTGTGATGATGACTAAGGAGTTTTTTCCTGTTGCTAAAGGCATGAACTTTATGGATTCGAGCGCTCAAAGTTCAAGGAGCACCGTTGATATTTCCTTCCAGAGGGGGAAACAAGGTGGAGACTTTATAGGTAGCGGGAGCGGTGGTGGCGATGCTTCACGGGTGATGCAGCCACCATCACAGCCGGTGAAGAAAAGCAGGAGAGGTCCTAGGTCTAAGAGTTCTCAGTATAGAGGCGTCACTTTCTATCGGAGGACAGGCAGATGGGAATCGCATATCTGGGATTGCGGTAAACAAGTTTATTTAGGTGGATTTGATACTGCCCATGCTGCAGCTAGGGCGTATGATCGAGCTGCTGTCAAATTCCGGGGTCTGGAAGCTGACATCAATTTCGTTATTGGCGATTATGAAGAGGATCTCAAGCAGATGGCGAATCTTTCCAAAGAGGAAGTTGTGCAAGTACTTCGGCGACAGAGCTCTGGTTTCTCAAGGAATAATTCTAGATATCAAGGAGTTGCTTTGCAAAAGATTGGCGGGTGGGGAGCTCAAATGGAGCAGCTTCATGGAAACATGGGTTGTGACAAGGCAGCCGTACAATGGAAGGGAAGGGAAGCTGCTTCCTTAATTGAGCCTCATGCATCCCGAATGATTCCCGAGGCAGCTAATGTTAAGCTCGACCTCAACTTGGGAATCTCTCTTTCACTAGGAGATGGTCCGAAGCAAAAAGATAGGGCCCTCCGGCTTCACCATGTCCCTAACAATTCCGTATGTGGAAGGAACACCATGATGGAGAACCACATGGCTGCAGCGGCATGTGATACGCCTTTCAATTTCTTGAAGAGGGGTTCAGACCATCTTAATAACCGGCATGCCCTTCCTTCTGCGTTCTTTTCACCCATGGAAAGAACACCAGAGAAAGGGCTTATGTTACGTTCTCATCAAAGCTTCCCAGCCAGGACATGGCAAGGGCATGATCAGTCCAGTGGTGGGACCGCCGTAGCAGCTACAGCACCGCCACTGTTTTCAAATGCAGCATCATCAGGATTCTCACTCTCAGCTACACGCCCTCCTTCATCCACAGCTATTCATCATCCTTCTCAGCCCTTCGTCAATCTGAATCAGCCCGGTCTCTATGTTATCCACCCATCTGATTACATATCTCAGCACCAACACAATCTCATGAACCGCCCACAACCACCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

485

Amino Acids

53.16

Weight (kDa)

6.77

Isoelectric Point (pI)

55.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 160 - 208 1.2e-09 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020264)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60120 AT5G60120 AT5G60120 AT5G60120 AT5G60120 AT5G60120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 24, 110
AccB7I CCANNNNNTGG 1 cut(s) 1212
AccBSI CCGCTC 1 cut(s) 393
AccI GTMKAC 2 cut(s) 24, 38
AciI CCGC 8 cut(s) 387, 393, 540, 786, 1061, 1245, 1265, 1438
AclWI GGATC 2 cut(s) 15, 675
AcsI RAATTY 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 153
AcyI GRCGYC 3 cut(s) 21, 107, 489
AfaI GTAC 2 cut(s) 714, 846
AfeI AGCGCT 1 cut(s) 316
AfiI CCNNNNNNNGG 7 cut(s) 354, 625, 849, 937, 1169, 1212, 1239
AgsI TTSAA 6 cut(s) 85, 194, 327, 1081, 1090, 1278
AjnI CCWGG 1 cut(s) 1205
Alw21I GWGCWC 4 cut(s) 173, 335, 731, 799
Alw26I GTCTC 2 cut(s) 366, 1385
AlwI GGATC 2 cut(s) 15, 675
Ama87I CYCGRG 1 cut(s) 905
Aor51HI AGCGCT 1 cut(s) 316
AoxI GGCC 1 cut(s) 984
ApaI GGGCCC 1 cut(s) 988
ApoI RAATTY 1 cut(s) 614
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AspA2I CCTAGG 1 cut(s) 461
AspLEI GCGC 1 cut(s) 317
AspS9I GGNCC 5 cut(s) 458, 965, 984, 985, 1242
AsuC2I CCSGG 2 cut(s) 620, 1377
AsuHPI GGTGA 6 cut(s) 115, 167, 427, 451, 989, 1137
AvaI CYCGRG 1 cut(s) 905
AvaII GGWCC 3 cut(s) 458, 965, 1242
AvrII CCTAGG 1 cut(s) 461
BaeGI GKGCMC 1 cut(s) 988
BanII GRGCYC 3 cut(s) 731, 799, 988
Bbv12I GWGCWC 4 cut(s) 173, 335, 731, 799
BccI CCATC 9 cut(s) 85, 146, 436, 510, 673, 956, 1033, 1113, 1405
BceAI ACGGC 2 cut(s) 827, 1232
BcgI CGANNNNNNTGC 2 cut(s) 698, 732
BciT130I CCWGG 1 cut(s) 1207
BclI TGATCA 1 cut(s) 1225
BcnI CCSGG 2 cut(s) 620, 1377
BcoDI GTCTC 2 cut(s) 366, 1385
BfaI CTAG 5 cut(s) 141, 462, 588, 753, 956
BfmI CTRYAG 3 cut(s) 582, 1056, 1257
BfoI RGCGCY 1 cut(s) 318
BlnI CCTAGG 1 cut(s) 461
BmcAI AGTACT 1 cut(s) 714
Bme1390I CCNGG 3 cut(s) 620, 1207, 1377
Bme18I GGWCC 3 cut(s) 458, 965, 1242
BmeT110I CYCGRG 1 cut(s) 905
BmgT120I GGNCC 5 cut(s) 458, 965, 984, 985, 1242
BmiI GGNNCC 2 cut(s) 986, 1243
BmrFI CCNGG 3 cut(s) 620, 1207, 1377
BmsI GCATC 4 cut(s) 394, 408, 899, 1295
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpuEI CTTGAG 2 cut(s) 656, 724
BpuMI CCSGG 2 cut(s) 620, 1377
BsaBI GATNNNNATC 2 cut(s) 12, 520
BsaHI GRCGYC 3 cut(s) 21, 107, 489
BsaI GGTCTC 1 cut(s) 1385
BsaJI CCNNGG 4 cut(s) 461, 619, 906, 1149
BsaXI ACNNNNNCTCC 4 cut(s) 382, 412, 1303, 1333
Bsc4I CCNNNNNNNGG 7 cut(s) 354, 625, 849, 937, 1169, 1212, 1239
Bse118I RCCGGY 2 cut(s) 436, 1116
Bse1I ACTGG 1 cut(s) 1233
Bse8I GATNNNNATC 2 cut(s) 12, 520
BseBI CCWGG 1 cut(s) 1207
BseDI CCNNGG 4 cut(s) 461, 619, 906, 1149
BseGI GGATG 4 cut(s) 100, 890, 1325, 1342
BseJI GATNNNNATC 2 cut(s) 12, 520
BseLI CCNNNNNNNGG 7 cut(s) 354, 625, 849, 937, 1169, 1212, 1239
BseMII CTCAG 5 cut(s) 56, 491, 1320, 1364, 1427
BseNI ACTGG 1 cut(s) 1233
BseRI GAGGAG 2 cut(s) 228, 231
BseSI GKGCMC 1 cut(s) 988
BseYI CCCAGC 1 cut(s) 1201
Bsh1285I CGRYCG 1 cut(s) 33
BshFI GGCC 1 cut(s) 986
BsiEI CGRYCG 1 cut(s) 33
BsiHKAI GWGCWC 4 cut(s) 173, 335, 731, 799
BsiHKCI CYCGRG 1 cut(s) 905
BsiSI CCGG 5 cut(s) 437, 619, 991, 1117, 1377
BslFI GGGAC 2 cut(s) 989, 1255
BslI CCNNNNNNNGG 7 cut(s) 354, 625, 849, 937, 1169, 1212, 1239
BsmAI GTCTC 2 cut(s) 366, 1385
BsmFI GGGAC 2 cut(s) 989, 1255
BsnI GGCC 1 cut(s) 986
Bso31I GGTCTC 1 cut(s) 1385
BsoBI CYCGRG 1 cut(s) 905
Bsp120I GGGCCC 1 cut(s) 984
Bsp1286I GDGCHC 5 cut(s) 173, 335, 731, 799, 988
Bsp143I GATC 4 cut(s) 7, 598, 667, 1225
Bsp19I CCATGG 1 cut(s) 1149
BspACI CCGC 8 cut(s) 387, 393, 540, 786, 1061, 1245, 1265, 1438
BspANI GGCC 1 cut(s) 986
BspCNI CTCAG 5 cut(s) 55, 490, 1319, 1363, 1426
BspHI TCATGA 1 cut(s) 1431
BspLI GGNNCC 2 cut(s) 986, 1243
BspMAI CTGCAG 2 cut(s) 586, 1060
BspPI GGATC 2 cut(s) 15, 675
BspTNI GGTCTC 1 cut(s) 1385
BsrBI CCGCTC 1 cut(s) 393
BsrFI RCCGGY 2 cut(s) 436, 1116
BsrI ACTGG 1 cut(s) 1233
BssAI RCCGGY 2 cut(s) 436, 1116
BssECI CCNNGG 4 cut(s) 461, 619, 906, 1149
BssMI GATC 4 cut(s) 7, 598, 667, 1225
BssNI GRCGYC 3 cut(s) 21, 107, 489
BssT1I CCWWGG 2 cut(s) 461, 1149
Bst2UI CCWGG 1 cut(s) 1207
Bst4CI ACNGT 4 cut(s) 67, 184, 337, 1272
Bst6I CTCTTC 3 cut(s) 121, 657, 1085
BstACI GRCGYC 3 cut(s) 21, 107, 489
BstC8I GCNNGC 1 cut(s) 1122
BstDEI CTNAG 7 cut(s) 42, 267, 468, 477, 1306, 1350, 1413
BstDSI CCRYGG 1 cut(s) 1149
BstENI CCTNNNNNAGG 1 cut(s) 352
BstF5I GGATG 4 cut(s) 100, 890, 1325, 1342
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 1 cut(s) 317
BstKTI GATC 4 cut(s) 10, 601, 670, 1228
BstMAI GTCTC 2 cut(s) 366, 1385
BstMBI GATC 4 cut(s) 7, 598, 667, 1225
BstMCI CGRYCG 1 cut(s) 33
BstMWI GCNNNNNNNGC 6 cut(s) 399, 581, 590, 1061, 1253, 1259
BstNI CCWGG 1 cut(s) 1207
BstNSI RCATGY 2 cut(s) 1068, 1124
BstSCI CCNGG 3 cut(s) 618, 1205, 1375
BstSFI CTRYAG 3 cut(s) 582, 1056, 1257
BstSLI GKGCMC 1 cut(s) 988
BstX2I RGATCY 2 cut(s) 7, 667
BstYI RGATCY 2 cut(s) 7, 667
BsuRI GGCC 1 cut(s) 986
BtgI CCRYGG 1 cut(s) 1149
BtgZI GCGATG 1 cut(s) 417
BtsCI GGATG 4 cut(s) 100, 890, 1325, 1342
BtsIMutI CAGTG 2 cut(s) 1240, 1268
Cac8I GCNNGC 1 cut(s) 1122
CciI TCATGA 1 cut(s) 1431
CfoI GCGC 1 cut(s) 317
Cfr10I RCCGGY 2 cut(s) 436, 1116
Cfr13I GGNCC 5 cut(s) 458, 965, 984, 985, 1242
CseI GACGC 1 cut(s) 478
Csp6I GTAC 2 cut(s) 713, 845
CviQI GTAC 2 cut(s) 713, 845
DdeI CTNAG 7 cut(s) 42, 267, 468, 477, 1306, 1350, 1413
DpnI GATC 4 cut(s) 9, 600, 669, 1227
DpnII GATC 4 cut(s) 7, 598, 667, 1225
Eam1104I CTCTTC 3 cut(s) 121, 657, 1085
EarI CTCTTC 3 cut(s) 121, 657, 1085
Ecl136II GAGCTC 2 cut(s) 729, 797
Eco130I CCWWGG 2 cut(s) 461, 1149
Eco24I GRGCYC 3 cut(s) 731, 799, 988
Eco31I GGTCTC 1 cut(s) 1385
Eco32I GATATC 1 cut(s) 758
Eco47I GGWCC 3 cut(s) 458, 965, 1242
Eco47III AGCGCT 1 cut(s) 316
Eco53kI GAGCTC 2 cut(s) 729, 797
Eco57I CTGAAG 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 905
EcoICRI GAGCTC 2 cut(s) 729, 797
EcoNI CCTNNNNNAGG 1 cut(s) 352
EcoO109I RGGNCCY 3 cut(s) 458, 984, 985
EcoRII CCWGG 1 cut(s) 1205
EcoRV GATATC 1 cut(s) 758
EcoT14I CCWWGG 2 cut(s) 461, 1149
EcoT22I ATGCAT 1 cut(s) 892
EcoT38I GRGCYC 3 cut(s) 731, 799, 988
ErhI CCWWGG 2 cut(s) 461, 1149
FaqI GGGAC 2 cut(s) 989, 1255
FauI CCCGC 2 cut(s) 380, 779
FbaI TGATCA 1 cut(s) 1225
FblI GTMKAC 2 cut(s) 24, 38
FokI GGATG 4 cut(s) 107, 877, 1312, 1329
FriOI GRGCYC 3 cut(s) 731, 799, 988
FspBI CTAG 5 cut(s) 141, 462, 588, 753, 956
GlaI GCGC 1 cut(s) 316
GsaI CCCAGC 1 cut(s) 1205
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 1 cut(s) 986
HapII CCGG 5 cut(s) 437, 619, 991, 1117, 1377
HgaI GACGC 1 cut(s) 478
HhaI GCGC 1 cut(s) 317
Hin1I GRCGYC 3 cut(s) 21, 107, 489
Hin6I GCGC 1 cut(s) 315
HinP1I GCGC 1 cut(s) 315
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 2 cut(s) 135, 1195
HpaII CCGG 5 cut(s) 437, 619, 991, 1117, 1377
HphI GGTGA 6 cut(s) 115, 167, 427, 451, 989, 1137
Hpy166II GTNNAC 3 cut(s) 25, 39, 545
Hpy188I TCNGA 7 cut(s) 45, 90, 504, 969, 1102, 1368, 1402
Hpy188III TCNNGA 9 cut(s) 17, 352, 626, 753, 894, 905, 1087, 1293, 1432
Hpy8I GTNNAC 3 cut(s) 25, 39, 545
Hpy99I CGWCG 3 cut(s) 23, 26, 112
HpyAV CCTTC 9 cut(s) 358, 847, 852, 1020, 1136, 1140, 1332, 1356, 1366
HpyCH4III ACNGT 4 cut(s) 67, 184, 337, 1272
HpyCH4IV ACGT 3 cut(s) 21, 107, 1183
HpyCH4V TGCA 7 cut(s) 421, 584, 709, 775, 890, 1058, 1283
HpyF10VI GCNNNNNNNGC 6 cut(s) 399, 581, 590, 1061, 1253, 1259
HpyF3I CTNAG 7 cut(s) 42, 267, 468, 477, 1306, 1350, 1413
HpySE526I ACGT 3 cut(s) 21, 107, 1183
Hsp92I GRCGYC 3 cut(s) 21, 107, 489
HspAI GCGC 1 cut(s) 315
Ksp22I TGATCA 1 cut(s) 1225
Kzo9I GATC 4 cut(s) 7, 598, 667, 1225
LmnI GCTCC 4 cut(s) 330, 390, 794, 805
LweI GCATC 4 cut(s) 394, 408, 899, 1295
MaeI CTAG 5 cut(s) 141, 462, 588, 753, 956
MaeII ACGT 3 cut(s) 21, 107, 1183
MaeIII GTNAC 4 cut(s) 103, 490, 830, 1179
MalI GATC 4 cut(s) 9, 600, 669, 1227
MbiI CCGCTC 1 cut(s) 393
MboI GATC 4 cut(s) 7, 598, 667, 1225
MflI RGATCY 2 cut(s) 7, 667
MhlI GDGCHC 5 cut(s) 173, 335, 731, 799, 988
MluCI AATT 7 cut(s) 194, 614, 640, 748, 876, 1012, 1081
MlyI GAGTC 2 cut(s) 20, 44
Mph1103I ATGCAT 1 cut(s) 892
MseI TTAA 5 cut(s) 69, 126, 875, 921, 1110
MslI CAYNNNNRTG 1 cut(s) 1037
MspA1I CMGCKG 1 cut(s) 1061
MspI CCGG 5 cut(s) 437, 619, 991, 1117, 1377
MspR9I CCNGG 3 cut(s) 620, 1207, 1377
MvaI CCWGG 1 cut(s) 1207
MwoI GCNNNNNNNGC 6 cut(s) 399, 581, 590, 1061, 1253, 1259
NciI CCSGG 2 cut(s) 620, 1377
NcoI CCATGG 1 cut(s) 1149
NdeII GATC 4 cut(s) 7, 598, 667, 1225
NlaIV GGNNCC 2 cut(s) 986, 1243
NmuCI GTSAC 3 cut(s) 103, 490, 830
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 2 cut(s) 1068, 1124
PaeI GCATGC 1 cut(s) 1124
PagI TCATGA 1 cut(s) 1431
PcsI WCGNNNNNNNCGW 3 cut(s) 30, 107, 651
PflFI GACNNNGTC 1 cut(s) 34
PflMI CCANNNNNTGG 1 cut(s) 1212
PleI GAGTC 2 cut(s) 20, 43
PpsI GAGTC 2 cut(s) 20, 43
PpuMI RGGWCCY 1 cut(s) 458
Psp124BI GAGCTC 2 cut(s) 731, 799
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 1205
PspFI CCCAGC 1 cut(s) 1201
PspGI CCWGG 1 cut(s) 1205
PspN4I GGNNCC 2 cut(s) 986, 1243
PspOMI GGGCCC 1 cut(s) 984
PspPI GGNCC 5 cut(s) 458, 965, 984, 985, 1242
PspPPI RGGWCCY 1 cut(s) 458
PstI CTGCAG 2 cut(s) 586, 1060
PsuI RGATCY 2 cut(s) 7, 667
PsyI GACNNNGTC 1 cut(s) 34
RsaI GTAC 2 cut(s) 714, 846
RsaNI GTAC 2 cut(s) 713, 845
RseI CAYNNNNRTG 1 cut(s) 1037
SacI GAGCTC 2 cut(s) 731, 799
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 5 cut(s) 69, 126, 875, 921, 1110
Sau3AI GATC 4 cut(s) 7, 598, 667, 1225
Sau96I GGNCC 5 cut(s) 458, 965, 984, 985, 1242
ScaI AGTACT 1 cut(s) 714
SchI GAGTC 2 cut(s) 20, 44
ScrFI CCNGG 3 cut(s) 620, 1207, 1377
SduI GDGCHC 5 cut(s) 173, 335, 731, 799, 988
SfaNI GCATC 4 cut(s) 394, 408, 899, 1295
SfcI CTRYAG 3 cut(s) 582, 1056, 1257
SinI GGWCC 3 cut(s) 458, 965, 1242
SmiMI CAYNNNNRTG 1 cut(s) 1037
SmlI CTYRAG 2 cut(s) 671, 739
SmoI CTYRAG 2 cut(s) 671, 739
SphI GCATGC 1 cut(s) 1124
Sse9I AATT 7 cut(s) 194, 614, 640, 748, 876, 1012, 1081
SsiI CCGC 8 cut(s) 387, 393, 540, 786, 1061, 1245, 1265, 1438
SspMI CTAG 5 cut(s) 141, 462, 588, 753, 956
SstI GAGCTC 2 cut(s) 731, 799
StyD4I CCNGG 3 cut(s) 618, 1205, 1375
StyI CCWWGG 2 cut(s) 461, 1149
TaaI ACNGT 4 cut(s) 67, 184, 337, 1272
TaiI ACGT 3 cut(s) 24, 110, 1186
TaqI TCGA 8 cut(s) 18, 24, 29, 59, 110, 311, 601, 927
TaqII GACCGA 1 cut(s) 47
TasI AATT 7 cut(s) 194, 614, 640, 748, 876, 1012, 1081
TatI WGTACW 1 cut(s) 712
TauI GCSGC 1 cut(s) 1064
Tru1I TTAA 5 cut(s) 69, 126, 875, 921, 1110
Tru9I TTAA 5 cut(s) 69, 126, 875, 921, 1110
TscAI CASTG 2 cut(s) 1240, 1275
TseFI GTSAC 3 cut(s) 103, 490, 830
Tsp45I GTSAC 3 cut(s) 103, 490, 830
TspDTI ATGAA 8 cut(s) 111, 258, 311, 675, 803, 1314, 1328, 1448
TspGWI ACGGA 1 cut(s) 1006
TspRI CASTG 2 cut(s) 1240, 1275
Tth111I GACNNNGTC 1 cut(s) 34
Van91I CCANNNNNTGG 1 cut(s) 1212
VpaK11BI GGWCC 3 cut(s) 458, 965, 1242
XagI CCTNNNNNAGG 1 cut(s) 352
XapI RAATTY 1 cut(s) 614
XbaI TCTAGA 1 cut(s) 752
XceI RCATGY 2 cut(s) 1068, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 1209
XmaJI CCTAGG 1 cut(s) 461
XmiI GTMKAC 2 cut(s) 24, 38
XspI CTAG 5 cut(s) 141, 462, 588, 753, 956
ZraI GACGTC 2 cut(s) 22, 108
ZrmI AGTACT 1 cut(s) 714
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.