FvH4_2g26161

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0027601
Physical Location & Seq
Reverse (-)
21042520 .. 21045182
2663 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26161.t1

Sequence Viewer

Length: 1650 bp
ATGGCCATACTTTATCTTAAATGGAGGAAAACTAGTGCATCACAGGTTGAAAATAAGTTGGACATCAGTATACAATTTCTGACATTGTTCTTGCGTCTGATGCCTCAGTCATCTCGTCCTATGGATATCTCAAAGATCACAGAAGAAAACATCAGCAACATGATTATGGTGAAGCTTAATCGGTCCAACTACACACTGTGGAGGTTACGTTTTCTTCGAGTTCTGAAGAAGTACAAAGTGTATGGACTTGTTGAAGGTTCTGATTCATGTCTTCCAGCCTTTGTAATTAACAGTCGGGGATATATCACTGATGTTATCAATCCTGCATATGATATTTGGATGGAACGTGACCGGATTGTAAAGCTCTGGTTGACTTCAACTATCACTGAGGACTTGCTACATAACATGGCTGGAAAAACTTCTGCTATGGATTTATGGTTGACTTTGGAGAAGAGGTCTCTTGGTGCAGCAAGGTCACATACACATGATCAACTTCGATCACAACTTGAAACTATCAGCAAGGGAAACATGTCCATGAATGAATATCTTCAACAGATCAAGCAGCTAGGGGATGGTTTAGCAGCAGTTGGAGCTCCTATTTATGGCGTTGAACTTATCCAGTATGTGCTTAATGGCTTACCTGAGGAATATGAGCCTTTTGTTATTGATACTGTGTTGCGTGTTCATCGGACTAGTATTGATGAACTGTCCAATCTGTTGCTTAATAAGGAGGGGTACATGGTACAAAATCGTATGTCACCTACTTCTCCAGAGCCTCTTCACGGCCAGGCCAATGTGCTAGAAATTTGCCCAGTAGGAACCAGAGCAGAAATTAAACTGGCTGCAATGAACTTTTCTCCTAATAGCTGGAGAAGGGGTCAGCTTCTGGGTCGTGGAACTTTTGGGTCTGAGATTTCTCTTTTGAGTCGGCTTCAACATGAGAACATAATTCAATACTATGGCTCATATGAGCATGAATCGAACCTCTATATGTGTATGGAGCTTGCACCTCATGGATCCCTGCGAAAATACTATCAGACATGCCATCTTTCAGGCCCTCGAGTCTCTGTATACACGAAAAAGATCCTGCTTGGTCTCAAGTATCTCCATGACCAAAATGTGGTTCATAGGGACATTAAATGTTCAAATATATTGGTGGATGCAAATCGATCTGTGAAGCTTGCAGATTTTGGACTTGCAAAGATAACCAAAATGAACGACATTCAATCTTTGCAGGGGAGCGTATTCTGGATGGCCCCTGAGGTTGTTAATGCAAATATGATAAACCAAGGTTATGGGCTTCCAGCTGATATATGGAGCCTTGGATGCACTGTGTTGGAGACATTCACCGGCCGCGTTCCATATCATAACCTCGAATGGTGTCAGGCATTATTCAAGATTGGTAAGGGGGAGCCCCCAAAGGTTCCTGATTCTCTTCCAAAGGATGCACAAGATTTCATCCACAAATGCCTACAAGTTAACCCAAATGATCGTCTCACCGCTGCGCAGCTCTTAGATCATCCATTTGTGAACATGCCACTAATTAATCCGACTTCTCCAAGGCTGCCAACGATATTTGATGATGTGCAACATTACAAGGGCATATACCGGTGTGCCTTCAACCCCGAGTCATGCACCAAATTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005768 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007584 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009611 GO:0009628 GO:0009631 GO:0009651 GO:0009888 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0009991 GO:0010033 GO:0010243 GO:0010449 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0012505 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0022622 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0031410 GO:0031667 GO:0031982 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035266 GO:0035556 GO:0036211 GO:0040007 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048364 GO:0048507 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048589 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0097159 GO:0097708 GO:0099402 GO:0140096 GO:1901363 GO:1901564 GO:1901698 GO:1901700 GO:1902065 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 ko04626 map04016 map04626
Pfam Domains
Protein Families
Pkinase

Protein Analysis

550

Amino Acids

62.52

Weight (kDa)

8.71

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotran_gag_2 PF14223 75 - 245 5.1e-12 gag-polypeptide of LTR copia-type
PK_Tyr_Ser-Thr PF07714 300 - 505 1.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 301 - 510 6.2e-60 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027601)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48050
fragaria_vesca FvH4_2g26161

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1506
AccB7I CCANNNNNTGG 1 cut(s) 1120
AccI GTMKAC 2 cut(s) 70, 1071
AccII CGCG 1 cut(s) 1356
AciI CCGC 2 cut(s) 1354, 1500
AclWI GGATC 3 cut(s) 1011, 1024, 1078
AcoI YGGCCR 3 cut(s) 3, 784, 1351
AcsI RAATTY 1 cut(s) 804
AcuI CTGAAG 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 198
AfaI GTAC 3 cut(s) 233, 737, 744
AfiI CCNNNNNNNGG 3 cut(s) 602, 782, 1120
AflIII ACRYGT 1 cut(s) 528
AgeI ACCGGT 1 cut(s) 1608
AhlI ACTAGT 2 cut(s) 32, 692
AjnI CCWGG 1 cut(s) 786
AjuI GAANNNNNNNTTGG 2 cut(s) 1107, 1139
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 5 cut(s) 462, 1069, 1100, 1334, 1499
AlwI GGATC 3 cut(s) 1011, 1024, 1078
AlwNI CAGNNNCTG 1 cut(s) 886
Ama87I CYCGRG 2 cut(s) 1059, 1625
AoxI GGCC 6 cut(s) 3, 784, 789, 1054, 1254, 1351
ApeKI GCWGC 7 cut(s) 467, 562, 581, 842, 1502, 1507, 1564
ApoI RAATTY 1 cut(s) 804
AseI ATTAAT 1 cut(s) 1545
AsiGI ACCGGT 1 cut(s) 1608
Asp700I GAANNNNTTC 2 cut(s) 418, 546
AspLEI GCGC 1 cut(s) 1507
AspS9I GGNCC 3 cut(s) 183, 1055, 1255
AsuHPI GGTGA 4 cut(s) 181, 750, 1339, 1489
AvaI CYCGRG 2 cut(s) 1059, 1625
AvaII GGWCC 1 cut(s) 183
AxyI CCTNAGG 2 cut(s) 642, 1260
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 1016
BanII GRGCYC 2 cut(s) 595, 1416
BbsI GAAGAC 1 cut(s) 263
Bbv12I GWGCWC 1 cut(s) 595
BbvI GCAGC 7 cut(s) 479, 574, 593, 829, 1489, 1519, 1551
BccI CCATC 4 cut(s) 334, 566, 1053, 1246
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 1 cut(s) 788
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 5 cut(s) 462, 1069, 1100, 1334, 1499
BcuI ACTAGT 2 cut(s) 32, 692
BfaI CTAG 4 cut(s) 33, 566, 693, 800
BisI GCNGC 8 cut(s) 468, 563, 582, 843, 1354, 1503, 1508, 1565
BlsI GCNGC 8 cut(s) 469, 564, 583, 844, 1355, 1504, 1509, 1566
Bme1390I CCNGG 1 cut(s) 788
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 2 cut(s) 1059, 1625
BmgT120I GGNCC 3 cut(s) 183, 1055, 1255
BmiI GGNNCC 6 cut(s) 820, 1018, 1257, 1319, 1413, 1425
BmrFI CCNGG 1 cut(s) 788
BmrI ACTGGG 1 cut(s) 806
BmsI GCATC 5 cut(s) 47, 90, 1150, 1316, 1435
BmuI ACTGGG 1 cut(s) 806
BpiI GAAGAC 1 cut(s) 263
BpmI CTGGAG 2 cut(s) 753, 889
BpuEI CTTGAG 1 cut(s) 1082
Bsa29I ATCGAT 1 cut(s) 1168
BsaBI GATNNNNATC 1 cut(s) 1164
BsaI GGTCTC 2 cut(s) 462, 1100
BsaJI CCNNGG 3 cut(s) 1288, 1321, 1559
BsaWI WCCGGW 2 cut(s) 351, 1608
BsaXI ACNNNNNCTCC 6 cut(s) 193, 223, 440, 470, 862, 892
Bsc4I CCNNNNNNNGG 3 cut(s) 602, 782, 1120
Bse118I RCCGGY 2 cut(s) 1349, 1608
Bse1I ACTGG 3 cut(s) 619, 812, 843
Bse21I CCTNAGG 2 cut(s) 642, 1260
Bse3DI GCAATG 1 cut(s) 852
Bse8I GATNNNNATC 1 cut(s) 1164
BseBI CCWGG 1 cut(s) 788
BseCI ATCGAT 1 cut(s) 1168
BseDI CCNNGG 3 cut(s) 1288, 1321, 1559
BseGI GGATG 8 cut(s) 345, 577, 1165, 1257, 1331, 1450, 1458, 1519
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 3 cut(s) 602, 782, 1120
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 5 cut(s) 119, 378, 633, 900, 1251
BseNI ACTGG 3 cut(s) 619, 812, 843
BseX3I CGGCCG 1 cut(s) 1351
BseXI GCAGC 7 cut(s) 479, 574, 593, 829, 1489, 1519, 1551
BsgI GTGCAG 1 cut(s) 486
Bsh1236I CGCG 1 cut(s) 1356
Bsh1285I CGRYCG 1 cut(s) 1354
BshFI GGCC 6 cut(s) 5, 786, 791, 1056, 1256, 1353
BshTI ACCGGT 1 cut(s) 1608
BshVI ATCGAT 1 cut(s) 1168
BsiEI CGRYCG 1 cut(s) 1354
BsiHKAI GWGCWC 1 cut(s) 595
BsiHKCI CYCGRG 2 cut(s) 1059, 1625
BsiSI CCGG 3 cut(s) 352, 1350, 1609
BslFI GGGAC 1 cut(s) 1145
BslI CCNNNNNNNGG 3 cut(s) 602, 782, 1120
BsmAI GTCTC 5 cut(s) 462, 1069, 1100, 1334, 1499
BsmBI CGTCTC 1 cut(s) 1499
BsmFI GGGAC 1 cut(s) 1145
BsnI GGCC 6 cut(s) 5, 786, 791, 1056, 1256, 1353
Bso31I GGTCTC 2 cut(s) 462, 1100
BsoBI CYCGRG 2 cut(s) 1059, 1625
Bsp1286I GDGCHC 2 cut(s) 595, 1416
Bsp143I GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
BspACI CCGC 2 cut(s) 1354, 1500
BspANI GGCC 6 cut(s) 5, 786, 791, 1056, 1256, 1353
BspCNI CTCAG 5 cut(s) 118, 379, 634, 901, 1252
BspDI ATCGAT 1 cut(s) 1168
BspFNI CGCG 1 cut(s) 1356
BspLI GGNNCC 6 cut(s) 820, 1018, 1257, 1319, 1413, 1425
BspPI GGATC 3 cut(s) 1011, 1024, 1078
BspTNI GGTCTC 2 cut(s) 462, 1100
BsrDI GCAATG 1 cut(s) 852
BsrFI RCCGGY 2 cut(s) 1349, 1608
BsrI ACTGG 3 cut(s) 619, 812, 843
BssAI RCCGGY 2 cut(s) 1349, 1608
BssECI CCNNGG 3 cut(s) 1288, 1321, 1559
BssMI GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
BssNAI GTATAC 2 cut(s) 71, 1072
BssT1I CCWWGG 3 cut(s) 1288, 1321, 1559
Bst1107I GTATAC 2 cut(s) 71, 1072
Bst2UI CCWGG 1 cut(s) 788
Bst4CI ACNGT 5 cut(s) 198, 293, 673, 708, 1333
Bst6I CTCTTC 3 cut(s) 446, 783, 1440
BstC8I GCNNGC 2 cut(s) 1005, 1182
BstDEI CTNAG 6 cut(s) 105, 387, 642, 909, 1260, 1513
BstF5I GGATG 8 cut(s) 345, 577, 1165, 1257, 1331, 1450, 1458, 1519
BstFNI CGCG 1 cut(s) 1356
BstHHI GCGC 1 cut(s) 1507
BstKTI GATC 9 cut(s) 138, 490, 500, 558, 1019, 1086, 1172, 1492, 1519
BstMAI GTCTC 5 cut(s) 462, 1069, 1100, 1334, 1499
BstMBI GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
BstMCI CGRYCG 1 cut(s) 1354
BstMWI GCNNNNNNNGC 3 cut(s) 100, 590, 1326
BstNI CCWGG 1 cut(s) 788
BstNSI RCATGY 3 cut(s) 532, 1044, 1537
BstSCI CCNGG 1 cut(s) 786
BstUI CGCG 1 cut(s) 1356
BstV1I GCAGC 7 cut(s) 479, 574, 593, 829, 1489, 1519, 1551
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 2 cut(s) 1016, 1083
BstXI CCANNNNNNTGG 1 cut(s) 1295
BstYI RGATCY 2 cut(s) 1016, 1083
BstZ17I GTATAC 2 cut(s) 71, 1072
BstZI CGGCCG 1 cut(s) 1351
Bsu15I ATCGAT 1 cut(s) 1168
Bsu36I CCTNAGG 2 cut(s) 642, 1260
BsuRI GGCC 6 cut(s) 5, 786, 791, 1056, 1256, 1353
BsuTUI ATCGAT 1 cut(s) 1168
BtsCI GGATG 8 cut(s) 345, 577, 1165, 1257, 1331, 1450, 1458, 1519
BtsIMutI CAGTG 4 cut(s) 194, 306, 384, 1329
Cac8I GCNNGC 2 cut(s) 1005, 1182
CaiI CAGNNNCTG 1 cut(s) 886
CfoI GCGC 1 cut(s) 1507
Cfr10I RCCGGY 2 cut(s) 1349, 1608
Cfr13I GGNCC 3 cut(s) 183, 1055, 1255
ClaI ATCGAT 1 cut(s) 1168
CseI GACGC 1 cut(s) 83
Csp6I GTAC 3 cut(s) 232, 736, 743
CspAI ACCGGT 1 cut(s) 1608
CviQI GTAC 3 cut(s) 232, 736, 743
DdeI CTNAG 6 cut(s) 105, 387, 642, 909, 1260, 1513
DpnI GATC 9 cut(s) 137, 489, 499, 557, 1018, 1085, 1171, 1491, 1518
DpnII GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
DraIII CACNNNGTG 1 cut(s) 198
EaeI YGGCCR 3 cut(s) 3, 784, 1351
EagI CGGCCG 1 cut(s) 1351
Eam1104I CTCTTC 3 cut(s) 446, 783, 1440
EarI CTCTTC 3 cut(s) 446, 783, 1440
Ecl136II GAGCTC 1 cut(s) 593
EclXI CGGCCG 1 cut(s) 1351
Eco130I CCWWGG 3 cut(s) 1288, 1321, 1559
Eco24I GRGCYC 2 cut(s) 595, 1416
Eco31I GGTCTC 2 cut(s) 462, 1100
Eco32I GATATC 1 cut(s) 127
Eco47I GGWCC 1 cut(s) 183
Eco52I CGGCCG 1 cut(s) 1351
Eco53kI GAGCTC 1 cut(s) 593
Eco57I CTGAAG 1 cut(s) 245
Eco81I CCTNAGG 2 cut(s) 642, 1260
Eco88I CYCGRG 2 cut(s) 1059, 1625
EcoICRI GAGCTC 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRII CCWGG 1 cut(s) 786
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 3 cut(s) 1288, 1321, 1559
EcoT38I GRGCYC 2 cut(s) 595, 1416
ErhI CCWWGG 3 cut(s) 1288, 1321, 1559
Esp3I CGTCTC 1 cut(s) 1499
FaqI GGGAC 1 cut(s) 1145
FauNDI CATATG 2 cut(s) 328, 967
FbaI TGATCA 1 cut(s) 487
FblI GTMKAC 2 cut(s) 70, 1071
Fnu4HI GCNGC 8 cut(s) 468, 563, 582, 843, 1354, 1503, 1508, 1565
FokI GGATG 8 cut(s) 352, 584, 1172, 1264, 1338, 1445, 1457, 1506
FriOI GRGCYC 2 cut(s) 595, 1416
Fsp4HI GCNGC 8 cut(s) 468, 563, 582, 843, 1354, 1503, 1508, 1565
FspBI CTAG 4 cut(s) 33, 566, 693, 800
FspI TGCGCA 1 cut(s) 1506
GlaI GCGC 1 cut(s) 1506
GluI GCNGC 8 cut(s) 468, 563, 582, 843, 1354, 1503, 1508, 1565
GsuI CTGGAG 2 cut(s) 753, 889
HaeIII GGCC 6 cut(s) 5, 786, 791, 1056, 1256, 1353
HapII CCGG 3 cut(s) 352, 1350, 1609
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 1507
Hin6I GCGC 1 cut(s) 1505
HinP1I GCGC 1 cut(s) 1505
HincII GTYRAC 3 cut(s) 372, 441, 1480
HindII GTYRAC 3 cut(s) 372, 441, 1480
HindIII AAGCTT 2 cut(s) 173, 1178
HinfI GANTC 6 cut(s) 263, 925, 977, 1062, 1430, 1628
HpaI GTTAAC 1 cut(s) 1480
HpaII CCGG 3 cut(s) 352, 1350, 1609
HphI GGTGA 4 cut(s) 181, 750, 1339, 1489
Hpy166II GTNNAC 6 cut(s) 71, 372, 441, 1072, 1480, 1531
Hpy188I TCNGA 8 cut(s) 81, 99, 225, 262, 690, 910, 1038, 1551
Hpy188III TCNNGA 4 cut(s) 770, 1249, 1396, 1427
Hpy8I GTNNAC 6 cut(s) 71, 372, 441, 1072, 1480, 1531
HpyAV CCTTC 3 cut(s) 248, 867, 1627
HpyCH4III ACNGT 5 cut(s) 198, 293, 673, 708, 1333
HpyCH4IV ACGT 2 cut(s) 208, 346
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 590, 1326
HpyF3I CTNAG 6 cut(s) 105, 387, 642, 909, 1260, 1513
HpySE526I ACGT 2 cut(s) 208, 346
HspAI GCGC 1 cut(s) 1505
Ksp22I TGATCA 1 cut(s) 487
KspAI GTTAAC 1 cut(s) 1480
Kzo9I GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
LmnI GCTCC 6 cut(s) 590, 598, 1000, 1239, 1317, 1411
Lsp1109I GCAGC 7 cut(s) 479, 574, 593, 829, 1489, 1519, 1551
LweI GCATC 5 cut(s) 47, 90, 1150, 1316, 1435
MaeI CTAG 4 cut(s) 33, 566, 693, 800
MaeII ACGT 2 cut(s) 208, 346
MaeIII GTNAC 4 cut(s) 204, 347, 474, 756
MalI GATC 9 cut(s) 137, 489, 499, 557, 1018, 1085, 1171, 1491, 1518
MboI GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
MboII GAAGA 8 cut(s) 155, 206, 238, 263, 463, 539, 770, 1427
MflI RGATCY 2 cut(s) 1016, 1083
MhlI GDGCHC 2 cut(s) 595, 1416
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 74, 285, 804, 831, 948, 1542, 1640
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 3 cut(s) 934, 1071, 1637
MmeI TCCRAC 5 cut(s) 39, 210, 568, 1317, 1574
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 418, 546
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 3 cut(s) 164, 483, 533
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 1307, 1502
MspI CCGG 3 cut(s) 352, 1350, 1609
MspR9I CCNGG 1 cut(s) 788
MvaI CCWGG 1 cut(s) 788
MvnI CGCG 1 cut(s) 1356
MwoI GCNNNNNNNGC 3 cut(s) 100, 590, 1326
NdeI CATATG 2 cut(s) 328, 967
NdeII GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
NlaIV GGNNCC 6 cut(s) 820, 1018, 1257, 1319, 1413, 1425
NmuCI GTSAC 3 cut(s) 347, 474, 756
NsbI TGCGCA 1 cut(s) 1506
NspI RCATGY 3 cut(s) 532, 1044, 1537
PaeR7I CTCGAG 1 cut(s) 1059
PciI ACATGT 1 cut(s) 528
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PdmI GAANNNNTTC 2 cut(s) 418, 546
PfeI GAWTC 3 cut(s) 263, 977, 1430
PflMI CCANNNNNTGG 1 cut(s) 1120
PinAI ACCGGT 1 cut(s) 1608
PkrI GCNGC 8 cut(s) 469, 564, 583, 844, 1355, 1504, 1509, 1566
PleI GAGTC 3 cut(s) 933, 1070, 1636
PpsI GAGTC 3 cut(s) 933, 1070, 1636
PscI ACATGT 1 cut(s) 528
PshBI ATTAAT 1 cut(s) 1545
Psp124BI GAGCTC 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 786
PspGI CCWGG 1 cut(s) 786
PspN4I GGNNCC 6 cut(s) 820, 1018, 1257, 1319, 1413, 1425
PspPI GGNCC 3 cut(s) 183, 1055, 1255
PspXI VCTCGAGB 1 cut(s) 1059
PstNI CAGNNNCTG 1 cut(s) 886
PsuI RGATCY 2 cut(s) 1016, 1083
PvuII CAGCTG 1 cut(s) 1307
RsaI GTAC 3 cut(s) 233, 737, 744
RsaNI GTAC 3 cut(s) 232, 736, 743
RseI CAYNNNNRTG 3 cut(s) 164, 483, 533
SacI GAGCTC 1 cut(s) 595
SatI GCNGC 8 cut(s) 468, 563, 582, 843, 1354, 1503, 1508, 1565
Sau3AI GATC 9 cut(s) 135, 487, 497, 555, 1016, 1083, 1169, 1489, 1516
Sau96I GGNCC 3 cut(s) 183, 1055, 1255
SchI GAGTC 3 cut(s) 934, 1071, 1637
ScrFI CCNGG 1 cut(s) 788
SduI GDGCHC 2 cut(s) 595, 1416
SfaNI GCATC 5 cut(s) 47, 90, 1150, 1316, 1435
Sfr274I CTCGAG 1 cut(s) 1059
SinI GGWCC 1 cut(s) 183
SlaI CTCGAG 1 cut(s) 1059
SmiMI CAYNNNNRTG 3 cut(s) 164, 483, 533
SmlI CTYRAG 2 cut(s) 1059, 1097
SmoI CTYRAG 2 cut(s) 1059, 1097
SpeI ACTAGT 2 cut(s) 32, 692
Sse9I AATT 7 cut(s) 74, 285, 804, 831, 948, 1542, 1640
SsiI CCGC 2 cut(s) 1354, 1500
SspMI CTAG 4 cut(s) 33, 566, 693, 800
SstI GAGCTC 1 cut(s) 595
StyD4I CCNGG 1 cut(s) 786
StyI CCWWGG 3 cut(s) 1288, 1321, 1559
TaaI ACNGT 5 cut(s) 198, 293, 673, 708, 1333
TaiI ACGT 2 cut(s) 211, 349
TaqI TCGA 6 cut(s) 217, 496, 980, 1060, 1168, 1374
TaqII GACCGA 1 cut(s) 171
TasI AATT 7 cut(s) 74, 285, 804, 831, 948, 1542, 1640
TatI WGTACW 1 cut(s) 231
TauI GCSGC 1 cut(s) 1356
TfiI GAWTC 3 cut(s) 263, 977, 1430
TscAI CASTG 4 cut(s) 201, 313, 391, 1336
TseFI GTSAC 3 cut(s) 347, 474, 756
TseI GCWGC 7 cut(s) 467, 562, 581, 842, 1502, 1507, 1564
Tsp45I GTSAC 3 cut(s) 347, 474, 756
TspRI CASTG 4 cut(s) 201, 313, 391, 1336
Van91I CCANNNNNTGG 1 cut(s) 1120
VpaK11BI GGWCC 1 cut(s) 183
VspI ATTAAT 1 cut(s) 1545
XapI RAATTY 1 cut(s) 804
XceI RCATGY 3 cut(s) 532, 1044, 1537
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XhoI CTCGAG 1 cut(s) 1059
XmiI GTMKAC 2 cut(s) 70, 1071
XmnI GAANNNNTTC 2 cut(s) 418, 546
XspI CTAG 4 cut(s) 33, 566, 693, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.