FvH4_2g39151

histone H3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0026660
Physical Location & Seq
Reverse (-)
28196215 .. 28196768
554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39151.t1

Sequence Viewer

Length: 429 bp
ATGGCTCGTACGAAGCAGACCGCTCGCAAATCCACCGGCGGGAAGGCTCCGAGGAAGCAGCTGGCCACCAAGGCAGCCCGGAAGTCAGCTCCGACGACCGGCGGAGTGAAGAAGCCGCATCGCTACCGCCCCGGAACCGTCGCCCTCCGTGAGATCCGCAAGTACCAGAAGAGCACCGAGCTTCTGATACGGAAGCTTCCTTTCCAGCGCCTGGTTCGTGAGATCGCTCAGGATTTCAAGACGGACTTGAGGTTTCAGAGCCACGCCGTGCTTGCGCTTCAGGAGGCGGCGGAGGCTTACTTGGTGGGCCTGTTTGAGGATACTAATCTGTGCGCGATTCATGCAAAGAGGGTGACGATTATGCCCAAGGATATTCAACTTGCGAGGAGGATTCGTGGCGAGCATTTGGTTGTTGCTTATCTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

143

Amino Acids

16.09

Weight (kDa)

10.88

Isoelectric Point (pI)

36.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 44 - 131 1.2e-38 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026660)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g39151
pyrus_communis pycom01g06240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 211
AccBSI CCGCTC 1 cut(s) 23
AccII CGCG 1 cut(s) 335
AciI CCGC 8 cut(s) 21, 39, 102, 116, 127, 157, 287, 290
AclWI GGATC 1 cut(s) 148
AcoI YGGCCR 1 cut(s) 63
AcuI CTGAAG 1 cut(s) 263
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 2 cut(s) 10, 164
AfiI CCNNNNNNNGG 4 cut(s) 39, 98, 211, 316
AgsI TTSAA 2 cut(s) 238, 377
AjnI CCWGG 1 cut(s) 210
AluBI AGCT 4 cut(s) 61, 89, 181, 196
AluI AGCT 4 cut(s) 61, 89, 181, 196
Alw21I GWGCWC 1 cut(s) 176
AlwI GGATC 1 cut(s) 148
AlwNI CAGNNNCTG 1 cut(s) 211
AoxI GGCC 2 cut(s) 63, 307
ApeKI GCWGC 2 cut(s) 58, 74
AspLEI GCGC 3 cut(s) 210, 277, 335
AspS9I GGNCC 1 cut(s) 307
AsuC2I CCSGG 2 cut(s) 79, 132
AsuHPI GGTGA 1 cut(s) 364
BalI TGGCCA 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 176
BbvI GCAGC 2 cut(s) 70, 86
BceAI ACGGC 1 cut(s) 251
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 1 cut(s) 212
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 2 cut(s) 79, 132
BfoI RGCGCY 1 cut(s) 211
BfuI GTATCC 1 cut(s) 313
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 4 cut(s) 59, 75, 116, 288
BlsI GCNGC 4 cut(s) 60, 76, 117, 289
Bme1390I CCNGG 3 cut(s) 79, 132, 212
BmgT120I GGNCC 1 cut(s) 307
BmiI GGNNCC 2 cut(s) 48, 136
BmrFI CCNGG 3 cut(s) 79, 132, 212
BmsI GCATC 1 cut(s) 127
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 268
BpuMI CCSGG 2 cut(s) 79, 132
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 50, 69, 130, 366
BsaXI ACNNNNNCTCC 2 cut(s) 379, 409
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 98, 211, 316
Bse118I RCCGGY 2 cut(s) 35, 98
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 4 cut(s) 50, 69, 130, 366
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 4 cut(s) 39, 98, 211, 316
BseMII CTCAG 1 cut(s) 242
BseRI GAGGAG 1 cut(s) 400
BseXI GCAGC 2 cut(s) 70, 86
Bsh1236I CGCG 1 cut(s) 335
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 2 cut(s) 65, 309
BsiEI CGRYCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 176
BsiSI CCGG 4 cut(s) 36, 79, 99, 132
BsiWI CGTACG 1 cut(s) 8
BslI CCNNNNNNNGG 4 cut(s) 39, 98, 211, 316
BsnI GGCC 2 cut(s) 65, 309
Bsp1286I GDGCHC 1 cut(s) 176
Bsp143I GATC 2 cut(s) 153, 222
BspACI CCGC 8 cut(s) 21, 39, 102, 116, 127, 157, 287, 290
BspANI GGCC 2 cut(s) 65, 309
BspCNI CTCAG 1 cut(s) 241
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 2 cut(s) 48, 136
BspPI GGATC 1 cut(s) 148
BspQI GCTCTTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 35, 98
BssAI RCCGGY 2 cut(s) 35, 98
BssECI CCNNGG 4 cut(s) 50, 69, 130, 366
BssMI GATC 2 cut(s) 153, 222
BssT1I CCWWGG 2 cut(s) 69, 366
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 164
BstC8I GCNNGC 4 cut(s) 25, 63, 273, 401
BstDEI CTNAG 1 cut(s) 228
BstENI CCTNNNNNAGG 1 cut(s) 314
BstFNI CGCG 1 cut(s) 335
BstH2I RGCGCY 1 cut(s) 211
BstHHI GCGC 3 cut(s) 210, 277, 335
BstKTI GATC 2 cut(s) 156, 225
BstMBI GATC 2 cut(s) 153, 222
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
BstNI CCWGG 1 cut(s) 212
BstSCI CCNGG 3 cut(s) 77, 130, 210
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 2 cut(s) 70, 86
BstX2I RGATCY 1 cut(s) 153
BstYI RGATCY 1 cut(s) 153
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 2 cut(s) 65, 309
BtgZI GCGATG 1 cut(s) 104
Cac8I GCNNGC 4 cut(s) 25, 63, 273, 401
CaiI CAGNNNCTG 1 cut(s) 211
CfoI GCGC 3 cut(s) 210, 277, 335
Cfr10I RCCGGY 2 cut(s) 35, 98
Cfr13I GGNCC 1 cut(s) 307
Csp6I GTAC 2 cut(s) 9, 163
CviAII CATG 1 cut(s) 341
CviQI GTAC 2 cut(s) 9, 163
DdeI CTNAG 1 cut(s) 228
DpnI GATC 2 cut(s) 155, 224
DpnII GATC 2 cut(s) 153, 222
DraIII CACNNNGTG 1 cut(s) 268
EaeI YGGCCR 1 cut(s) 63
Eam1104I CTCTTC 1 cut(s) 164
EarI CTCTTC 1 cut(s) 164
EciI GGCGGA 2 cut(s) 117, 305
Eco130I CCWWGG 2 cut(s) 69, 366
Eco57I CTGAAG 1 cut(s) 263
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 2 cut(s) 69, 366
ErhI CCWWGG 2 cut(s) 69, 366
FaeI CATG 1 cut(s) 344
FaiI YATR 2 cut(s) 342, 362
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 32
Fnu4HI GCNGC 4 cut(s) 59, 75, 116, 288
Fsp4HI GCNGC 4 cut(s) 59, 75, 116, 288
GlaI GCGC 3 cut(s) 209, 276, 334
GluI GCNGC 4 cut(s) 59, 75, 116, 288
HaeII RGCGCY 1 cut(s) 211
HaeIII GGCC 2 cut(s) 65, 309
HapII CCGG 4 cut(s) 36, 79, 99, 132
HhaI GCGC 3 cut(s) 210, 277, 335
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 3 cut(s) 208, 275, 333
HinP1I GCGC 3 cut(s) 208, 275, 333
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 337, 391
HpaII CCGG 4 cut(s) 36, 79, 99, 132
HphI GGTGA 1 cut(s) 364
Hpy188I TCNGA 4 cut(s) 51, 93, 186, 258
Hpy188III TCNNGA 4 cut(s) 218, 230, 238, 281
Hpy99I CGWCG 2 cut(s) 97, 143
HpyAV CCTTC 1 cut(s) 37
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4V TGCA 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
HpyF3I CTNAG 1 cut(s) 228
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 3 cut(s) 208, 275, 333
Kzo9I GATC 2 cut(s) 153, 222
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 52, 94
Lsp1109I GCAGC 2 cut(s) 70, 86
LweI GCATC 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 155, 224
MbiI CCGCTC 1 cut(s) 23
MboI GATC 2 cut(s) 153, 222
MboII GAAGA 2 cut(s) 121, 181
MflI RGATCY 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 176
MlsI TGGCCA 1 cut(s) 65
MluNI TGGCCA 1 cut(s) 65
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 9 cut(s) 45, 155, 243, 277, 286, 310, 342, 378, 381
Mox20I TGGCCA 1 cut(s) 65
MscI TGGCCA 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 65
MspA1I CMGCKG 1 cut(s) 61
MspI CCGG 4 cut(s) 36, 79, 99, 132
MspR9I CCNGG 3 cut(s) 79, 132, 212
MvaI CCWGG 1 cut(s) 212
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 4 cut(s) 71, 272, 293, 341
NciI CCSGG 2 cut(s) 79, 132
NdeII GATC 2 cut(s) 153, 222
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 48, 136
NmuCI GTSAC 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 164
PfeI GAWTC 2 cut(s) 337, 391
Pfl23II CGTACG 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 211
PkrI GCNGC 4 cut(s) 60, 76, 117, 289
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 48, 136
PspPI GGNCC 1 cut(s) 307
PstNI CAGNNNCTG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 61
RsaI GTAC 2 cut(s) 10, 164
RsaNI GTAC 2 cut(s) 9, 163
SapI GCTCTTC 1 cut(s) 164
SatI GCNGC 4 cut(s) 59, 75, 116, 288
Sau3AI GATC 2 cut(s) 153, 222
Sau96I GGNCC 1 cut(s) 307
ScrFI CCNGG 3 cut(s) 79, 132, 212
SduI GDGCHC 1 cut(s) 176
SetI ASST 5 cut(s) 63, 91, 183, 198, 254
SfaNI GCATC 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
SsiI CCGC 8 cut(s) 21, 39, 102, 116, 127, 157, 287, 290
StyD4I CCNGG 3 cut(s) 77, 130, 210
StyI CCWWGG 2 cut(s) 69, 366
TaaI ACNGT 1 cut(s) 139
TauI GCSGC 2 cut(s) 118, 290
TfiI GAWTC 2 cut(s) 337, 391
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 58, 74
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 329
TspGWI ACGGA 3 cut(s) 137, 205, 257
Van91I CCANNNNNTGG 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.