FvH4_3g10463

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0027783
Physical Location & Seq
Reverse (-)
6145210 .. 6147743
2534 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g10463.t1

Sequence Viewer

Length: 1053 bp
ATGACTCACATATCCCTTGAAGGGGAGTTGCAAATAATTCAAGAAATAGCTTATGATTGTTACAATGACATGAACGAGCCCTTGTTCAGCAACAACCCCTCGCTCTCGCTGCCTACTCCTTATACAATTTCCGATACCAAAAACAAGTTCATTGTGGTTGGTTGTGATGCTTTAGCACTATTTCAAGGCCACCGGGGGGATGAGAACTACACGACCGGGTGTATGTCAATATGTGACGACAATGTTGGCAGTGCTAATGCATCTTGCTCTGGTATTGGGTGCTGCCAAACTTCCATCCCTAGTGGTCTTCAGAGTCTTGGTATGAAAGTGAGTAGCTTTTACAATCACTCGTACATAACAAGCCACTGCGGCTATGCCTTGATAGTGCAAGAAGAAAAATTCACATACTCTGGGAATAAAAGCATTACAGATCTATTAGGCATTGAACAGCTTCCAATGGTTGTTAACTGGGGAATTGGAGATGCCCCATGTGATGCAGCTGAAAAGAAGCTGGATTATGCATGCAAGAAAAATAGTGAGTGTGTCAACCGGACCATCACTATTGGGTCTGGGTCGTCTGGTTACTTTGGCCAGTGCTTGCCAGGTTATGAAGGGAACCCATATCTTCCAGATGGTTGCCAAGATATTGATGAGTGCGAGGCTAACCCCTGCAATGGACTGAATGGAGTATGTCTAAATACACCAGGGAATTACTCTTGTTTATGTAGCAAAGGCTACAAAAACAATGGCCCGAATGGAAAGTGCATCAAAGACAATTCCAGCAACCGTCTCCTGCTTATAGTTCTTTCGTTGGGGGAGGAAAGGCCTACCATGAGAGAAGTAGCCATGGAGCTAGAAGGAATGATTATGACAAAGCATCCATGGGGTTCTGCAGGTCATAACTTCCCAGAAGAGACTATGCACTTGCTGGGATCACCTAATACTTTCATGGATTACATTGTCAATGTTGATGGCGATGGTGGTCCTGGTACAACCAGTGGGTGTGACAGCATGCAACTCGAATTGTTAAATTCATATGCTGATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

351

Amino Acids

37.73

Weight (kDa)

4.55

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EGF_CA PF07645 215 - 255 2.6e-08 Calcium-binding EGF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027783)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10463
prunus_persica Prupe.4G093600_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 884
AciI CCGC 1 cut(s) 369
AclWI GGATC 1 cut(s) 940
AcoI YGGCCR 1 cut(s) 589
AcsI RAATTY 2 cut(s) 398, 1030
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 2 cut(s) 353, 991
AfiI CCNNNNNNNGG 4 cut(s) 21, 22, 196, 674
AgsI TTSAA 4 cut(s) 20, 41, 185, 446
AjnI CCWGG 3 cut(s) 601, 703, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 131, 163
AluBI AGCT 6 cut(s) 50, 336, 451, 500, 511, 853
AluI AGCT 6 cut(s) 50, 336, 451, 500, 511, 853
Alw26I GTCTC 2 cut(s) 794, 908
AlwI GGATC 1 cut(s) 940
AoxI GGCC 4 cut(s) 187, 589, 748, 824
ApeKI GCWGC 3 cut(s) 109, 282, 497
ApoI RAATTY 2 cut(s) 398, 1030
Asp700I GAANNNNTTC 1 cut(s) 450
AspS9I GGNCC 3 cut(s) 552, 749, 983
AsuC2I CCSGG 2 cut(s) 194, 217
AsuHPI GGTGA 1 cut(s) 927
AvaII GGWCC 2 cut(s) 552, 983
BalI TGGCCA 1 cut(s) 591
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 1 cut(s) 299
BbvI GCAGC 3 cut(s) 96, 269, 509
BccI CCATC 6 cut(s) 302, 563, 626, 965, 971, 1037
BcgI CGANNNNNNTGC 2 cut(s) 1000, 1034
BciT130I CCWGG 3 cut(s) 603, 705, 987
BcnI CCSGG 2 cut(s) 194, 217
BcoDI GTCTC 2 cut(s) 794, 908
BfaI CTAG 2 cut(s) 300, 854
BfmI CTRYAG 1 cut(s) 891
BfuAI ACCTGC 1 cut(s) 884
BglI GCCNNNNNGGC 1 cut(s) 369
BglII AGATCT 1 cut(s) 430
BisI GCNGC 4 cut(s) 110, 283, 370, 498
BlsI GCNGC 4 cut(s) 111, 284, 371, 499
Bme1390I CCNGG 5 cut(s) 194, 217, 603, 705, 987
Bme18I GGWCC 2 cut(s) 552, 983
BmgT120I GGNCC 3 cut(s) 552, 749, 983
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 5 cut(s) 194, 217, 603, 705, 987
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 6 cut(s) 157, 269, 472, 484, 774, 886
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 299
BpuMI CCSGG 2 cut(s) 194, 217
BsaJI CCNNGG 4 cut(s) 193, 704, 846, 881
BsaWI WCCGGW 1 cut(s) 549
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 22, 196, 674
Bse1I ACTGG 3 cut(s) 473, 592, 996
Bse3DI GCAATG 1 cut(s) 679
BseBI CCWGG 3 cut(s) 603, 705, 987
BseDI CCNNGG 4 cut(s) 193, 704, 846, 881
BseGI GGATG 3 cut(s) 205, 294, 877
BseLI CCNNNNNNNGG 4 cut(s) 21, 22, 196, 674
BseMI GCAATG 1 cut(s) 679
BseNI ACTGG 3 cut(s) 473, 592, 996
BseXI GCAGC 3 cut(s) 96, 269, 509
BseYI CCCAGC 1 cut(s) 928
Bsh1285I CGRYCG 1 cut(s) 216
BshFI GGCC 4 cut(s) 189, 591, 750, 826
BsiEI CGRYCG 1 cut(s) 216
BsiSI CCGG 3 cut(s) 193, 216, 550
BslI CCNNNNNNNGG 4 cut(s) 21, 22, 196, 674
BsmAI GTCTC 2 cut(s) 794, 908
BsmBI CGTCTC 1 cut(s) 794
BsnI GGCC 4 cut(s) 189, 591, 750, 826
Bsp1286I GDGCHC 1 cut(s) 81
Bsp143I GATC 2 cut(s) 430, 932
Bsp19I CCATGG 2 cut(s) 846, 881
BspACI CCGC 1 cut(s) 369
BspANI GGCC 4 cut(s) 189, 591, 750, 826
BspLI GGNNCC 1 cut(s) 617
BspMAI CTGCAG 1 cut(s) 895
BspMI ACCTGC 1 cut(s) 884
BspPI GGATC 1 cut(s) 940
BsrDI GCAATG 1 cut(s) 679
BsrI ACTGG 3 cut(s) 473, 592, 996
BssECI CCNNGG 4 cut(s) 193, 704, 846, 881
BssMI GATC 2 cut(s) 430, 932
BssT1I CCWWGG 2 cut(s) 846, 881
Bst2UI CCWGG 3 cut(s) 603, 705, 987
Bst4CI ACNGT 1 cut(s) 788
Bst6I CTCTTC 1 cut(s) 906
BstC8I GCNNGC 3 cut(s) 523, 599, 1013
BstDSI CCRYGG 2 cut(s) 846, 881
BstF5I GGATG 3 cut(s) 205, 294, 877
BstKTI GATC 2 cut(s) 433, 935
BstMAI GTCTC 2 cut(s) 794, 908
BstMBI GATC 2 cut(s) 430, 932
BstMCI CGRYCG 1 cut(s) 216
BstMWI GCNNNNNNNGC 2 cut(s) 109, 369
BstNI CCWGG 3 cut(s) 603, 705, 987
BstNSI RCATGY 2 cut(s) 525, 1015
BstSCI CCNGG 5 cut(s) 192, 215, 601, 703, 985
BstSFI CTRYAG 1 cut(s) 891
BstV1I GCAGC 3 cut(s) 96, 269, 509
BstV2I GAAGAC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 4 cut(s) 189, 591, 750, 826
BtgI CCRYGG 2 cut(s) 846, 881
BtgZI GCGATG 1 cut(s) 990
BtsCI GGATG 3 cut(s) 205, 294, 877
BtsI GCAGTG 2 cut(s) 256, 364
BtsIMutI CAGTG 4 cut(s) 256, 364, 599, 1003
BveI ACCTGC 1 cut(s) 884
Cac8I GCNNGC 3 cut(s) 523, 599, 1013
Cfr13I GGNCC 3 cut(s) 552, 749, 983
Csp6I GTAC 2 cut(s) 352, 990
CviAII CATG 8 cut(s) 70, 489, 522, 832, 847, 882, 949, 1012
CviQI GTAC 2 cut(s) 352, 990
DpnI GATC 2 cut(s) 432, 934
DpnII GATC 2 cut(s) 430, 932
EaeI YGGCCR 1 cut(s) 589
Eam1104I CTCTTC 1 cut(s) 906
EarI CTCTTC 1 cut(s) 906
Eco130I CCWWGG 2 cut(s) 846, 881
Eco147I AGGCCT 1 cut(s) 826
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 552, 983
Eco57I CTGAAG 1 cut(s) 293
EcoRII CCWGG 3 cut(s) 601, 703, 985
EcoT14I CCWWGG 2 cut(s) 846, 881
EcoT22I ATGCAT 2 cut(s) 262, 523
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 846, 881
Esp3I CGTCTC 1 cut(s) 794
FaeI CATG 8 cut(s) 73, 492, 525, 835, 850, 885, 952, 1015
FatI CATG 8 cut(s) 69, 488, 521, 831, 846, 881, 948, 1011
FauNDI CATATG 1 cut(s) 1036
Fnu4HI GCNGC 4 cut(s) 110, 283, 370, 498
FokI GGATG 3 cut(s) 212, 281, 864
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 110, 283, 370, 498
FspBI CTAG 2 cut(s) 300, 854
GluI GCNGC 4 cut(s) 110, 283, 370, 498
GsaI CCCAGC 1 cut(s) 932
HaeIII GGCC 4 cut(s) 189, 591, 750, 826
HapII CCGG 3 cut(s) 193, 216, 550
Hin1II CATG 8 cut(s) 73, 492, 525, 835, 850, 885, 952, 1015
HincII GTYRAC 2 cut(s) 466, 547
HindII GTYRAC 2 cut(s) 466, 547
HinfI GANTC 2 cut(s) 4, 313
HpaI GTTAAC 1 cut(s) 466
HpaII CCGG 3 cut(s) 193, 216, 550
HphI GGTGA 1 cut(s) 927
Hpy166II GTNNAC 2 cut(s) 466, 547
Hpy188I TCNGA 2 cut(s) 133, 312
Hpy188III TCNNGA 2 cut(s) 41, 629
Hpy8I GTNNAC 2 cut(s) 466, 547
HpyAV CCTTC 3 cut(s) 14, 605, 851
HpyCH4III ACNGT 1 cut(s) 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 369
Hsp92II CATG 8 cut(s) 73, 492, 525, 835, 850, 885, 952, 1015
KspAI GTTAAC 1 cut(s) 466
Kzo9I GATC 2 cut(s) 430, 932
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 3 cut(s) 96, 269, 509
LweI GCATC 6 cut(s) 157, 269, 472, 484, 774, 886
MaeI CTAG 2 cut(s) 300, 854
MaeIII GTNAC 4 cut(s) 59, 233, 581, 1004
MalI GATC 2 cut(s) 432, 934
MboI GATC 2 cut(s) 430, 932
MboII GAAGA 4 cut(s) 299, 404, 617, 923
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 1 cut(s) 81
MlsI TGGCCA 1 cut(s) 591
MluCI AATT 8 cut(s) 36, 126, 398, 474, 709, 775, 1022, 1030
MluNI TGGCCA 1 cut(s) 591
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 3 cut(s) 109, 652, 811
Mox20I TGGCCA 1 cut(s) 591
Mph1103I ATGCAT 2 cut(s) 262, 523
MroXI GAANNNNTTC 1 cut(s) 450
MscI TGGCCA 1 cut(s) 591
MseI TTAA 2 cut(s) 465, 1028
Msp20I TGGCCA 1 cut(s) 591
MspA1I CMGCKG 1 cut(s) 500
MspI CCGG 3 cut(s) 193, 216, 550
MspR9I CCNGG 5 cut(s) 194, 217, 603, 705, 987
MvaI CCWGG 3 cut(s) 603, 705, 987
MwoI GCNNNNNNNGC 2 cut(s) 109, 369
NciI CCSGG 2 cut(s) 194, 217
NcoI CCATGG 2 cut(s) 846, 881
NdeI CATATG 1 cut(s) 1036
NdeII GATC 2 cut(s) 430, 932
NlaIII CATG 8 cut(s) 73, 492, 525, 835, 850, 885, 952, 1015
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 2 cut(s) 233, 1004
NsiI ATGCAT 2 cut(s) 262, 523
NspI RCATGY 2 cut(s) 525, 1015
PaeI GCATGC 2 cut(s) 525, 1015
PceI AGGCCT 1 cut(s) 826
PdmI GAANNNNTTC 1 cut(s) 450
PkrI GCNGC 4 cut(s) 111, 284, 371, 499
PleI GAGTC 1 cut(s) 321
PpsI GAGTC 1 cut(s) 321
Psp6I CCWGG 3 cut(s) 601, 703, 985
PspFI CCCAGC 1 cut(s) 928
PspGI CCWGG 3 cut(s) 601, 703, 985
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 3 cut(s) 552, 749, 983
PstI CTGCAG 1 cut(s) 895
PsuI RGATCY 1 cut(s) 430
PvuII CAGCTG 1 cut(s) 500
RsaI GTAC 2 cut(s) 353, 991
RsaNI GTAC 2 cut(s) 352, 990
SaqAI TTAA 2 cut(s) 465, 1028
SatI GCNGC 4 cut(s) 110, 283, 370, 498
Sau3AI GATC 2 cut(s) 430, 932
Sau96I GGNCC 3 cut(s) 552, 749, 983
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 5 cut(s) 194, 217, 603, 705, 987
SduI GDGCHC 1 cut(s) 81
SetI ASST 9 cut(s) 52, 338, 453, 502, 513, 607, 855, 898, 940
SfaNI GCATC 6 cut(s) 157, 269, 472, 484, 774, 886
SfcI CTRYAG 1 cut(s) 891
SinI GGWCC 2 cut(s) 552, 983
SphI GCATGC 2 cut(s) 525, 1015
Sse9I AATT 8 cut(s) 36, 126, 398, 474, 709, 775, 1022, 1030
SseBI AGGCCT 1 cut(s) 826
SsiI CCGC 1 cut(s) 369
SspMI CTAG 2 cut(s) 300, 854
StuI AGGCCT 1 cut(s) 826
StyD4I CCNGG 5 cut(s) 192, 215, 601, 703, 985
StyI CCWWGG 2 cut(s) 846, 881
TaaI ACNGT 1 cut(s) 788
TaqI TCGA 1 cut(s) 1020
TasI AATT 8 cut(s) 36, 126, 398, 474, 709, 775, 1022, 1030
TauI GCSGC 1 cut(s) 372
Tru1I TTAA 2 cut(s) 465, 1028
Tru9I TTAA 2 cut(s) 465, 1028
TscAI CASTG 4 cut(s) 256, 371, 599, 1003
TseFI GTSAC 2 cut(s) 233, 1004
TseI GCWGC 3 cut(s) 109, 282, 497
Tsp45I GTSAC 2 cut(s) 233, 1004
TspDTI ATGAA 6 cut(s) 86, 139, 338, 624, 937, 1023
TspRI CASTG 4 cut(s) 256, 371, 599, 1003
VpaK11BI GGWCC 2 cut(s) 552, 983
XapI RAATTY 2 cut(s) 398, 1030
XceI RCATGY 2 cut(s) 525, 1015
XmnI GAANNNNTTC 1 cut(s) 450
XspI CTAG 2 cut(s) 300, 854
Zsp2I ATGCAT 2 cut(s) 262, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.