FvH4_4g23371

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0022220
Physical Location & Seq
Forward (+)
25880395 .. 25884554
4160 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23371.t1

Sequence Viewer

Length: 1002 bp
ATGCATAGAGCAAGGGAAATTGAATCAGACCTAGGTGTAGGAAAGTTGGATGAACTTCGTGTGACTGCCATTTGTGGGATGCCTGGAATTGAGGTCTATGAAAAGAAAGGTTTGTTCCATCTCCAGAAACAACTTTATGATGACTTTACGTTGAAGACCAAAGTAATCACGGTAATTGTCAACAATGTGAGGCAGAGATTGTGTAACAAAAGGGTGCTTATCATTCTTGATGATGTGAAGGAGTTGGAACATATAGTAGACCTAGTTGGAGAGGGCTCTGGTGAAATGCAGGATGTAATTGTGGATGCACGATTTACTACAACGAATTGTCCAGCTAATGATATGGGAACACAGCAGGTTGTGGGATCACATACAGATGTCATTCCTGTCCTTCAGAACAATAAGAACTTCAAGGACTGTGAATTCTTAACCAGGGCCCTTAACTTCAGTAGAACCCCGAGGCTGGAGAGGCTTCCTCTTATGAAATGGTGGTTCCAAACTGAAAAGGTCTCCGAAAATTGGAGAAAACATGGAATACTTACTGAAACTTCATTTAGATGGGACAGCTACATTAATAAGCATTTGACCAGCCGTTCTTTGCTCAAAATAAAAGACTGCAAGCTCCTGCGGAGTCTTTCTGATGTCATTTGTATGTGTGCATCACTCTCAAATCTACGTCAATTGCCAGATAACATGGGGAGCCTAGTGTGTTTGAAGGTGCTTGACATAAGTGGAAGTGGTATAAAGCAGATTCAGCAGCCAACAATTCCAGTGGTTTGCAGTTGTGCTCCGTTCTCTGGTTTATGCTCCTTGAGAGAATTAATTTCAAGCGATTGTAATCTGTCTGAAGGAGCAATCCCTGATGAAATTCGCTACATATCGTGGTTGAAGTATCTGGATTTAAGTGGAAACAACTTTGAGACATCACCCCAAACCATTTCCCAGCTTTATCAGCTTGAAGACCTTCTGCTAAATGATTTTAGCAACCTAAATCATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_8 NB-ARC TIR

Protein Analysis

334

Amino Acids

37.86

Weight (kDa)

6.19

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022220)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g23371 FvH4_4g23371
rosa_chinensis RchiOBHm_Chr4g0429691
rosa_rugosa Rorug01G0149200.1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccI GTMKAC 1 cut(s) 258
AciI CCGC 1 cut(s) 628
AclWI GGATC 1 cut(s) 373
AcsI RAATTY 2 cut(s) 422, 867
AcuI CTGAAG 3 cut(s) 377, 430, 867
AfiI CCNNNNNNNGG 4 cut(s) 75, 463, 519, 797
AgsI TTSAA 7 cut(s) 23, 154, 412, 715, 828, 889, 959
AjnI CCWGG 2 cut(s) 82, 431
AluBI AGCT 5 cut(s) 335, 567, 622, 946, 955
AluI AGCT 5 cut(s) 335, 567, 622, 946, 955
Alw21I GWGCWC 1 cut(s) 790
Alw26I GTCTC 2 cut(s) 514, 914
AlwI GGATC 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 457
AoxI GGCC 1 cut(s) 435
ApaI GGGCCC 1 cut(s) 439
ApeKI GCWGC 1 cut(s) 757
ApoI RAATTY 2 cut(s) 422, 867
AseI ATTAAT 2 cut(s) 573, 821
Asp700I GAANNNNTTC 1 cut(s) 963
AspA2I CCTAGG 1 cut(s) 31
AspS9I GGNCC 2 cut(s) 435, 436
AsuHPI GGTGA 2 cut(s) 293, 918
AvaI CYCGRG 1 cut(s) 457
AvrII CCTAGG 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 439
BanII GRGCYC 2 cut(s) 278, 439
BbsI GAAGAC 2 cut(s) 161, 966
Bbv12I GWGCWC 1 cut(s) 790
BbvI GCAGC 1 cut(s) 769
BccI CCATC 2 cut(s) 126, 552
BceAI ACGGC 1 cut(s) 576
BciT130I CCWGG 2 cut(s) 84, 433
BcoDI GTCTC 2 cut(s) 514, 914
BfaI CTAG 3 cut(s) 32, 263, 704
BfuAI ACCTGC 1 cut(s) 346
BisI GCNGC 1 cut(s) 758
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 1 cut(s) 759
Bme1390I CCNGG 2 cut(s) 84, 433
BmeT110I CYCGRG 1 cut(s) 457
BmgT120I GGNCC 2 cut(s) 435, 436
BmiI GGNNCC 3 cut(s) 437, 494, 701
BmrFI CCNGG 2 cut(s) 84, 433
BmsI GCATC 3 cut(s) 69, 295, 668
BpiI GAAGAC 2 cut(s) 161, 966
BplI GAGNNNNNCTC 2 cut(s) 460, 492
BpmI CTGGAG 2 cut(s) 107, 485
BpuEI CTTGAG 1 cut(s) 832
BsaBI GATNNNNATC 1 cut(s) 837
BsaI GGTCTC 1 cut(s) 514
BsaJI CCNNGG 3 cut(s) 31, 432, 458
Bsc4I CCNNNNNNNGG 4 cut(s) 75, 463, 519, 797
Bse1I ACTGG 1 cut(s) 770
Bse3DI GCAATG 1 cut(s) 994
Bse8I GATNNNNATC 1 cut(s) 837
BseBI CCWGG 2 cut(s) 84, 433
BseDI CCNNGG 3 cut(s) 31, 432, 458
BseGI GGATG 4 cut(s) 55, 84, 298, 310
BseJI GATNNNNATC 1 cut(s) 837
BseLI CCNNNNNNNGG 4 cut(s) 75, 463, 519, 797
BseMI GCAATG 1 cut(s) 994
BseNI ACTGG 1 cut(s) 770
BseSI GKGCMC 1 cut(s) 439
BseXI GCAGC 1 cut(s) 769
BseYI CCCAGC 1 cut(s) 942
BshFI GGCC 1 cut(s) 437
BsiHKAI GWGCWC 1 cut(s) 790
BsiHKCI CYCGRG 1 cut(s) 457
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 4 cut(s) 75, 463, 519, 797
BsmAI GTCTC 2 cut(s) 514, 914
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 1 cut(s) 437
Bso31I GGTCTC 1 cut(s) 514
BsoBI CYCGRG 1 cut(s) 457
Bsp120I GGGCCC 1 cut(s) 435
Bsp1286I GDGCHC 3 cut(s) 278, 439, 790
Bsp143I GATC 1 cut(s) 365
BspACI CCGC 1 cut(s) 628
BspANI GGCC 1 cut(s) 437
BspLI GGNNCC 3 cut(s) 437, 494, 701
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 1 cut(s) 373
BspTNI GGTCTC 1 cut(s) 514
BsrDI GCAATG 1 cut(s) 994
BsrI ACTGG 1 cut(s) 770
BssECI CCNNGG 3 cut(s) 31, 432, 458
BssMI GATC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 2 cut(s) 84, 433
Bst4CI ACNGT 2 cut(s) 172, 419
BstC8I GCNNGC 1 cut(s) 620
BstF5I GGATG 4 cut(s) 55, 84, 298, 310
BstKTI GATC 1 cut(s) 368
BstMAI GTCTC 2 cut(s) 514, 914
BstMBI GATC 1 cut(s) 365
BstMWI GCNNNNNNNGC 3 cut(s) 469, 754, 952
BstNI CCWGG 2 cut(s) 84, 433
BstSCI CCNGG 2 cut(s) 82, 431
BstSLI GKGCMC 1 cut(s) 439
BstV1I GCAGC 1 cut(s) 769
BstV2I GAAGAC 2 cut(s) 161, 966
BsuRI GGCC 1 cut(s) 437
BtsCI GGATG 4 cut(s) 55, 84, 298, 310
BtsIMutI CAGTG 1 cut(s) 777
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 620
Cfr13I GGNCC 2 cut(s) 435, 436
CspCI CAANNNNNGTGG 2 cut(s) 753, 788
CviAII CATG 2 cut(s) 530, 694
DpnI GATC 1 cut(s) 367
DpnII GATC 1 cut(s) 365
Eco130I CCWWGG 1 cut(s) 31
Eco24I GRGCYC 2 cut(s) 278, 439
Eco31I GGTCTC 1 cut(s) 514
Eco57I CTGAAG 3 cut(s) 377, 430, 867
Eco88I CYCGRG 1 cut(s) 457
EcoO109I RGGNCCY 2 cut(s) 435, 436
EcoRI GAATTC 1 cut(s) 422
EcoRII CCWGG 2 cut(s) 82, 431
EcoT14I CCWWGG 1 cut(s) 31
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 278, 439
ErhI CCWWGG 1 cut(s) 31
FaeI CATG 2 cut(s) 533, 697
FaqI GGGAC 1 cut(s) 575
FatI CATG 2 cut(s) 529, 693
FblI GTMKAC 1 cut(s) 258
Fnu4HI GCNGC 1 cut(s) 758
FokI GGATG 4 cut(s) 62, 91, 305, 317
FriOI GRGCYC 2 cut(s) 278, 439
Fsp4HI GCNGC 1 cut(s) 758
FspBI CTAG 3 cut(s) 32, 263, 704
GluI GCNGC 1 cut(s) 758
GsaI CCCAGC 1 cut(s) 946
GsuI CTGGAG 2 cut(s) 107, 485
HaeIII GGCC 1 cut(s) 437
Hin1II CATG 2 cut(s) 533, 697
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 3 cut(s) 23, 631, 751
HphI GGTGA 2 cut(s) 293, 918
Hpy166II GTNNAC 2 cut(s) 181, 259
Hpy188I TCNGA 5 cut(s) 28, 396, 514, 640, 847
Hpy188III TCNNGA 3 cut(s) 124, 227, 896
Hpy8I GTNNAC 2 cut(s) 181, 259
HpyAV CCTTC 5 cut(s) 232, 401, 709, 842, 974
HpyCH4III ACNGT 2 cut(s) 172, 419
HpyCH4IV ACGT 2 cut(s) 149, 676
HpyCH4V TGCA 6 cut(s) 4, 289, 308, 618, 659, 780
HpyF10VI GCNNNNNNNGC 3 cut(s) 469, 754, 952
HpySE526I ACGT 2 cut(s) 149, 676
Hsp92II CATG 2 cut(s) 533, 697
Kzo9I GATC 1 cut(s) 365
LmnI GCTCC 5 cut(s) 627, 699, 793, 812, 851
Lsp1109I GCAGC 1 cut(s) 769
LweI GCATC 3 cut(s) 69, 295, 668
MaeI CTAG 3 cut(s) 32, 263, 704
MaeII ACGT 2 cut(s) 149, 676
MaeIII GTNAC 2 cut(s) 61, 203
MalI GATC 1 cut(s) 367
MboI GATC 1 cut(s) 365
MboII GAAGA 2 cut(s) 166, 971
MfeI CAATTG 1 cut(s) 680
MhlI GDGCHC 3 cut(s) 278, 439, 790
MlyI GAGTC 1 cut(s) 640
MmeI TCCRAC 3 cut(s) 27, 225, 247
MnlI CCTC 6 cut(s) 85, 183, 265, 453, 462, 486
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 963
MseI TTAA 5 cut(s) 428, 441, 573, 821, 902
MslI CAYNNNNRTG 3 cut(s) 375, 556, 650
MspR9I CCNGG 2 cut(s) 84, 433
MunI CAATTG 1 cut(s) 680
MvaI CCWGG 2 cut(s) 84, 433
MwoI GCNNNNNNNGC 3 cut(s) 469, 754, 952
NdeII GATC 1 cut(s) 365
NlaIII CATG 2 cut(s) 533, 697
NlaIV GGNNCC 3 cut(s) 437, 494, 701
NmuCI GTSAC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 2 cut(s) 23, 751
PkrI GCNGC 1 cut(s) 759
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
PshBI ATTAAT 2 cut(s) 573, 821
Psp6I CCWGG 2 cut(s) 82, 431
PspFI CCCAGC 1 cut(s) 942
PspGI CCWGG 2 cut(s) 82, 431
PspN4I GGNNCC 3 cut(s) 437, 494, 701
PspOMI GGGCCC 1 cut(s) 435
PspPI GGNCC 2 cut(s) 435, 436
RseI CAYNNNNRTG 3 cut(s) 375, 556, 650
SaqAI TTAA 5 cut(s) 428, 441, 573, 821, 902
SatI GCNGC 1 cut(s) 758
Sau3AI GATC 1 cut(s) 365
Sau96I GGNCC 2 cut(s) 435, 436
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 2 cut(s) 84, 433
SduI GDGCHC 3 cut(s) 278, 439, 790
SfaNI GCATC 3 cut(s) 69, 295, 668
SmiMI CAYNNNNRTG 3 cut(s) 375, 556, 650
SmlI CTYRAG 1 cut(s) 811
SmoI CTYRAG 1 cut(s) 811
SsiI CCGC 1 cut(s) 628
SspMI CTAG 3 cut(s) 32, 263, 704
StyD4I CCNGG 2 cut(s) 82, 431
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 2 cut(s) 172, 419
TaiI ACGT 2 cut(s) 152, 679
TfiI GAWTC 2 cut(s) 23, 751
Tru1I TTAA 5 cut(s) 428, 441, 573, 821, 902
Tru9I TTAA 5 cut(s) 428, 441, 573, 821, 902
TscAI CASTG 1 cut(s) 777
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 757
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 5 cut(s) 66, 114, 497, 540, 879
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 1 cut(s) 777
VspI ATTAAT 2 cut(s) 573, 821
XapI RAATTY 2 cut(s) 422, 867
XmaJI CCTAGG 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 258
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 3 cut(s) 32, 263, 704
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.