FvH4_4g27600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0020864
Physical Location & Seq
Forward (+)
28532058 .. 28532950
893 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27600.t1

Sequence Viewer

Length: 309 bp
AGCGGTATTTATACAATCAAGCAAAACCCAGGCTCAGGCTGGGACCAAATAAAGAAAGAAAAGAAGAGGAAGAAGAAATTGCAGATCCAAAGGGCAGCAGGGAAGAGAAAGGAAGACAGGAGAAGAGAGATGGTGTTGAACAGCTATGTAGCGGTAAGAGTAGCAAACATGTCGGCATACGCGTGTCAATACATAGCTTGCAATCCCGAGAGATTGAGTAGCGACCAAGTTCTGTACCTCCTCTTCTGCTTCCCGTTCCTTCAACTCCGTCGCTTCCTCCTCAACTTCCGACGACGACCCTCCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

12.08

Weight (kDa)

10.54

Isoelectric Point (pI)

78.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55365
fragaria_vesca FvH4_4g27600
prunus_persica Prupe.7G004500_v2.0.a1
pyrus_communis pycom12g00340
rosa_rugosa Rorug03G0289200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AciI CCGC 2 cut(s) 3, 152
AclWI GGATC 1 cut(s) 79
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 1 cut(s) 35
AflIII ACRYGT 2 cut(s) 168, 180
AgsI TTSAA 2 cut(s) 139, 263
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 2 cut(s) 144, 197
AluI AGCT 2 cut(s) 144, 197
AlwI GGATC 1 cut(s) 79
Ama87I CYCGRG 1 cut(s) 206
ApeKI GCWGC 1 cut(s) 95
AspS9I GGNCC 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 43
BarI GAAGNNNNNNTAC 2 cut(s) 227, 259
BbsI GAAGAC 1 cut(s) 120
BbvI GCAGC 1 cut(s) 107
BccI CCATC 1 cut(s) 124
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 1 cut(s) 30
BisI GCNGC 1 cut(s) 96
BlsI GCNGC 1 cut(s) 97
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 1 cut(s) 44
BmrFI CCNGG 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 35
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 1 cut(s) 48
BseRI GAGGAG 2 cut(s) 230, 269
BseXI GCAGC 1 cut(s) 107
BseYI CCCAGC 1 cut(s) 39
Bsh1236I CGCG 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 206
Bsp143I GATC 1 cut(s) 84
BspACI CCGC 2 cut(s) 3, 152
BspCNI CTCAG 1 cut(s) 47
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 44
BspPI GGATC 1 cut(s) 79
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 30
Bst6I CTCTTC 4 cut(s) 59, 98, 118, 248
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 1 cut(s) 34
BstFNI CGCG 1 cut(s) 182
BstKTI GATC 1 cut(s) 87
BstMBI GATC 1 cut(s) 84
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 28
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 120
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 199
Cfr13I GGNCC 1 cut(s) 43
Csp6I GTAC 1 cut(s) 235
CviAII CATG 1 cut(s) 169
CviJI RGCY 4 cut(s) 33, 39, 144, 197
CviKI_1 RGCY 4 cut(s) 33, 39, 144, 197
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 1 cut(s) 34
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
Eam1104I CTCTTC 4 cut(s) 59, 98, 118, 248
EarI CTCTTC 4 cut(s) 59, 98, 118, 248
Eco47I GGWCC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 28
FaeI CATG 1 cut(s) 172
FaiI YATR 5 cut(s) 12, 147, 170, 178, 194
FaqI GGGAC 1 cut(s) 56
FatI CATG 1 cut(s) 168
Fnu4HI GCNGC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 96
GluI GCNGC 1 cut(s) 96
GsaI CCCAGC 1 cut(s) 43
Hin1II CATG 1 cut(s) 172
Hpy188I TCNGA 1 cut(s) 290
Hpy188III TCNNGA 1 cut(s) 206
Hpy99I CGWCG 3 cut(s) 273, 294, 297
HpyAV CCTTC 1 cut(s) 269
HpyCH4V TGCA 2 cut(s) 82, 201
HpyF3I CTNAG 1 cut(s) 34
Hsp92II CATG 1 cut(s) 172
Kzo9I GATC 1 cut(s) 84
LpnPI CCDG 6 cut(s) 15, 21, 25, 42, 84, 103
Lsp1109I GCAGC 1 cut(s) 107
MalI GATC 1 cut(s) 86
MboI GATC 1 cut(s) 84
MboII GAAGA 7 cut(s) 76, 82, 85, 115, 125, 135, 235
MflI RGATCY 1 cut(s) 84
MluCI AATT 1 cut(s) 77
MluI ACGCGT 1 cut(s) 180
MnlI CCTC 5 cut(s) 60, 248, 251, 287, 290
MslI CAYNNNNRTG 1 cut(s) 181
MspR9I CCNGG 1 cut(s) 30
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 182
NdeII GATC 1 cut(s) 84
NlaIII CATG 1 cut(s) 172
NlaIV GGNNCC 1 cut(s) 44
NspI RCATGY 1 cut(s) 172
PciI ACATGT 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 179
PkrI GCNGC 1 cut(s) 97
PscI ACATGT 1 cut(s) 168
Psp6I CCWGG 1 cut(s) 28
PspFI CCCAGC 1 cut(s) 39
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 44
PspPI GGNCC 1 cut(s) 43
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 1 cut(s) 181
SatI GCNGC 1 cut(s) 96
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 43
ScrFI CCNGG 1 cut(s) 30
SetI ASST 3 cut(s) 146, 199, 240
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 181
Sse9I AATT 1 cut(s) 77
SsiI CCGC 2 cut(s) 3, 152
StyD4I CCNGG 1 cut(s) 28
TasI AATT 1 cut(s) 77
TseI GCWGC 1 cut(s) 95
TspGWI ACGGA 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 43
XceI RCATGY 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.