MD01G1186900.v1.1

aminotransferase ACS12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0024384
Physical Location & Seq
Reverse (-)
28565640 .. 28566082
443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1186900.v1.1.491

Sequence Viewer

Length: 294 bp
ATGGGCCGGGTCCGGATGTCCTCCCGGGCGAGCTGGGTTGCGAAGCCCAATGACTCGCCGTACTATATCGGGTTGGATAGGGCTTCGGAGGAGCCGTACGATAGGGTGGATAATCCGGATGGGGTTATTCAACTCGGATTGTCTGAGAACAGGCTGTGTTTGGATTTGATTGAGAAATGGGTTTCGGAGAATCTGATGGAATCGATGGTGGGAACGGACGGTGGTGATTTGAGCATAAGTGGGATTGCAGCGTACCAGCCGTTTGATGGAATGACTAAGCTGAAAGTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

98

Amino Acids

10.73

Weight (kDa)

4.54

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024384)

Species Orthologous Gene IDs
malus_domestica MD01G1186700.v1.1 MD01G1186900.v1.1
pyrus_communis pycom01g19850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 12, 115
AfaI GTAC 3 cut(s) 62, 98, 254
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 131
AluBI AGCT 2 cut(s) 33, 280
AluI AGCT 2 cut(s) 33, 280
Ama87I CYCGRG 1 cut(s) 24
Aor13HI TCCGGA 2 cut(s) 12, 115
AoxI GGCC 1 cut(s) 4
ApeKI GCWGC 1 cut(s) 248
AspS9I GGNCC 2 cut(s) 4, 10
AsuC2I CCSGG 3 cut(s) 8, 25, 26
AsuHPI GGTGA 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 10
BbvI GCAGC 1 cut(s) 260
BccI CCATC 4 cut(s) 113, 190, 199, 260
BceAI ACGGC 3 cut(s) 43, 79, 244
BcnI CCSGG 3 cut(s) 8, 25, 26
BisI GCNGC 1 cut(s) 249
BlsI GCNGC 1 cut(s) 250
Bme1390I CCNGG 3 cut(s) 8, 25, 26
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 2 cut(s) 4, 10
BmiI GGNNCC 2 cut(s) 11, 93
BmrFI CCNGG 3 cut(s) 8, 25, 26
BpuMI CCSGG 3 cut(s) 8, 25, 26
Bsa29I ATCGAT 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 2 cut(s) 12, 115
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 1 cut(s) 266
BseAI TCCGGA 2 cut(s) 12, 115
BseCI ATCGAT 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 2 cut(s) 21, 124
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 135
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 260
BseYI CCCAGC 1 cut(s) 33
BshFI GGCC 1 cut(s) 6
BshVI ATCGAT 1 cut(s) 203
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 4 cut(s) 7, 13, 25, 116
BsiWI CGTACG 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 1 cut(s) 6
BsoBI CYCGRG 1 cut(s) 24
Bsp13I TCCGGA 2 cut(s) 12, 115
BspANI GGCC 1 cut(s) 6
BspCNI CTCAG 1 cut(s) 136
BspDI ATCGAT 1 cut(s) 203
BspEI TCCGGA 2 cut(s) 12, 115
BspLI GGNNCC 2 cut(s) 11, 93
BssECI CCNNGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 221
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 2 cut(s) 144, 276
BstF5I GGATG 2 cut(s) 21, 124
BstSCI CCNGG 3 cut(s) 6, 23, 24
BstV1I GCAGC 1 cut(s) 260
Bsu15I ATCGAT 1 cut(s) 203
BsuRI GGCC 1 cut(s) 6
BsuTUI ATCGAT 1 cut(s) 203
BtsCI GGATG 2 cut(s) 21, 124
Cac8I GCNNGC 1 cut(s) 31
Cfr13I GGNCC 2 cut(s) 4, 10
Cfr9I CCCGGG 1 cut(s) 24
ClaI ATCGAT 1 cut(s) 203
Csp6I GTAC 3 cut(s) 61, 97, 253
CviJI RGCY 8 cut(s) 6, 33, 46, 83, 94, 154, 259, 280
CviKI_1 RGCY 8 cut(s) 6, 33, 46, 83, 94, 154, 259, 280
CviQI GTAC 3 cut(s) 61, 97, 253
DdeI CTNAG 2 cut(s) 144, 276
Eco47I GGWCC 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 24
FaiI YATR 3 cut(s) 66, 236, 292
Fnu4HI GCNGC 1 cut(s) 249
FokI GGATG 2 cut(s) 28, 131
Fsp4HI GCNGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 249
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 1 cut(s) 6
HapII CCGG 4 cut(s) 7, 13, 25, 116
HinfI GANTC 3 cut(s) 53, 190, 200
HpaII CCGG 4 cut(s) 7, 13, 25, 116
HphI GGTGA 1 cut(s) 236
Hpy188I TCNGA 5 cut(s) 88, 137, 145, 187, 195
Hpy188III TCNNGA 2 cut(s) 13, 116
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 1 cut(s) 248
HpyF3I CTNAG 2 cut(s) 144, 276
Kpn2I TCCGGA 2 cut(s) 12, 115
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 7 cut(s) 19, 20, 26, 38, 129, 136, 269
Lsp1109I GCAGC 1 cut(s) 260
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 54
MnlI CCTC 2 cut(s) 31, 82
MroI TCCGGA 2 cut(s) 12, 115
MspI CCGG 4 cut(s) 7, 13, 25, 116
MspR9I CCNGG 3 cut(s) 8, 25, 26
NciI CCSGG 3 cut(s) 8, 25, 26
NlaIV GGNNCC 2 cut(s) 11, 93
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 2 cut(s) 190, 200
Pfl23II CGTACG 1 cut(s) 96
PkrI GCNGC 1 cut(s) 250
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PspFI CCCAGC 1 cut(s) 33
PspLI CGTACG 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 11, 93
PspPI GGNCC 2 cut(s) 4, 10
RsaI GTAC 3 cut(s) 62, 98, 254
RsaNI GTAC 3 cut(s) 61, 97, 253
SatI GCNGC 1 cut(s) 249
Sau96I GGNCC 2 cut(s) 4, 10
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 3 cut(s) 8, 25, 26
SetI ASST 2 cut(s) 35, 282
SinI GGWCC 1 cut(s) 10
SmaI CCCGGG 1 cut(s) 26
StyD4I CCNGG 3 cut(s) 6, 23, 24
TaaI ACNGT 1 cut(s) 221
TaqI TCGA 1 cut(s) 203
TfiI GAWTC 2 cut(s) 190, 200
TseI GCWGC 1 cut(s) 248
TspGWI ACGGA 1 cut(s) 230
TspMI CCCGGG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 10
XcmI CCANNNNNNNNNTGG 1 cut(s) 263
XmaI CCCGGG 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.