MD01G1233400.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0028616
Physical Location & Seq
Forward (+)
32135573 .. 32137651
2079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1233400.v1.1.491

Sequence Viewer

Length: 609 bp
ATGGAGAGAAAAGTGCTTCTGGTTTGCTGCGCTGTGGGACTCCTGGGGCTGCTATCTGCGGCTGCGGGTTTCGGCGCCGAGAGCACAAGAATCAAGGTTAAGTTTGTCTCGACTAGTCAATGTGAATACCCTGACACTCCGGCTTTTGGACTTGGTGTAACTGCTGCGGTGGCTCTTATGTTAGCTCAAATAATTATAAATGTTTCAACCGGGGGCAATTGTTGCAAAAGGAGGCCCCAGCCTTCCAACTCTAACTCGACGGTAGCACGGTTATACATTGTTTTTTCCAGTTTCTTACTATTCTTGTTGCTGCTGTTGTTTCTCTGGTTCGTATTCGTTATAGCGTTTTTTCTGCTGCTTGCTGGTGCCGCACTCAATCACCGGCATGGTGTAGGAAGCATGTACGCTGGCAACTACGACTGTTACGTTGTGAAACCCGGAGTGTTTGCTGGAGGTGCCCTCTTGTCATTTGCAAGTGCGGTACTCGGAATTGTCTACTATGTCAACTTAAACTCAGCAAAGAACAGTGATATGTCATGCGGCGGTTCTATCCCTAAAGGGGCAATAGCTATGGGGCAACCTCGGATCCCGCCAACCCAATGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

203

Amino Acids

21.26

Weight (kDa)

9.22

Isoelectric Point (pI)

24.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 55 - 160 4.9e-22 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028616)

Species Orthologous Gene IDs
malus_domestica MD01G1233400.v1.1
pyrus_communis pycom01g24170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
AccB1I GGYRCC 3 cut(s) 74, 365, 455
AccI GTMKAC 1 cut(s) 495
AciI CCGC 8 cut(s) 59, 65, 167, 369, 479, 540, 543, 590
AclWI GGATC 2 cut(s) 580, 593
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 404, 483
AfiI CCNNNNNNNGG 2 cut(s) 146, 559
AgsI TTSAA 1 cut(s) 207
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 2 cut(s) 185, 569
AluI AGCT 2 cut(s) 185, 569
Alw21I GWGCWC 1 cut(s) 86
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 580, 593
AoxI GGCC 1 cut(s) 233
ApeKI GCWGC 6 cut(s) 27, 49, 62, 164, 310, 355
AspLEI GCGC 2 cut(s) 32, 77
AspS9I GGNCC 1 cut(s) 234
AsuC2I CCSGG 2 cut(s) 211, 438
AsuHPI GGTGA 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 585
BanI GGYRCC 3 cut(s) 74, 365, 455
Bbv12I GWGCWC 1 cut(s) 86
BbvI GCAGC 6 cut(s) 14, 36, 49, 151, 297, 342
BciT130I CCWGG 1 cut(s) 44
BcnI CCSGG 2 cut(s) 211, 438
BcoDI GTCTC 1 cut(s) 112
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 78
BisI GCNGC 9 cut(s) 28, 50, 60, 63, 165, 311, 356, 369, 541
BlsI GCNGC 9 cut(s) 29, 51, 61, 64, 166, 312, 357, 370, 542
Bme1390I CCNGG 3 cut(s) 44, 211, 438
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 5 cut(s) 76, 236, 367, 457, 587
BmrFI CCNGG 3 cut(s) 44, 211, 438
BplI GAGNNNNNCTC 2 cut(s) 444, 476
BpmI CTGGAG 1 cut(s) 471
BpuMI CCSGG 2 cut(s) 211, 438
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 43, 210, 581
Bsc4I CCNNNNNNNGG 2 cut(s) 146, 559
Bse118I RCCGGY 1 cut(s) 381
Bse1I ACTGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 43, 210, 581
BseLI CCNNNNNNNGG 2 cut(s) 146, 559
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 288
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 6 cut(s) 14, 36, 49, 151, 297, 342
BseYI CCCAGC 1 cut(s) 237
BshFI GGCC 1 cut(s) 235
BshNI GGYRCC 3 cut(s) 74, 365, 455
BsiHKAI GWGCWC 1 cut(s) 86
BsiSI CCGG 4 cut(s) 140, 210, 382, 438
BslFI GGGAC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 146, 559
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 51
BsnI GGCC 1 cut(s) 235
Bsp1286I GDGCHC 2 cut(s) 86, 460
Bsp143I GATC 1 cut(s) 585
BspACI CCGC 8 cut(s) 59, 65, 167, 369, 479, 540, 543, 590
BspANI GGCC 1 cut(s) 235
BspCNI CTCAG 1 cut(s) 527
BspLI GGNNCC 5 cut(s) 76, 236, 367, 457, 587
BspPI GGATC 2 cut(s) 580, 593
BspT107I GGYRCC 3 cut(s) 74, 365, 455
BsrFI RCCGGY 1 cut(s) 381
BsrI ACTGG 1 cut(s) 288
BssAI RCCGGY 1 cut(s) 381
BssECI CCNNGG 3 cut(s) 43, 210, 581
BssMI GATC 1 cut(s) 585
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 4 cut(s) 262, 270, 422, 527
BstACI GRCGYC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 2 cut(s) 360, 409
BstDEI CTNAG 1 cut(s) 514
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 32, 77
BstKTI GATC 1 cut(s) 588
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 1 cut(s) 585
BstMWI GCNNNNNNNGC 5 cut(s) 81, 170, 222, 368, 455
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 3 cut(s) 42, 209, 436
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 6 cut(s) 14, 36, 49, 151, 297, 342
BstX2I RGATCY 1 cut(s) 585
BstYI RGATCY 1 cut(s) 585
BsuRI GGCC 1 cut(s) 235
BtsIMutI CAGTG 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 360, 409
CfoI GCGC 2 cut(s) 32, 77
Cfr10I RCCGGY 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 234
Csp6I GTAC 2 cut(s) 403, 482
CviAII CATG 3 cut(s) 386, 400, 537
CviJI RGCY 8 cut(s) 49, 62, 143, 173, 185, 235, 241, 569
CviKI_1 RGCY 8 cut(s) 49, 62, 143, 173, 185, 235, 241, 569
CviQI GTAC 2 cut(s) 403, 482
DdeI CTNAG 1 cut(s) 514
DinI GGCGCC 1 cut(s) 76
DpnI GATC 1 cut(s) 587
DpnII GATC 1 cut(s) 585
EcoO109I RGGNCCY 1 cut(s) 234
EcoRII CCWGG 1 cut(s) 42
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
FaeI CATG 3 cut(s) 389, 403, 540
FaqI GGGAC 1 cut(s) 51
FatI CATG 3 cut(s) 385, 399, 536
FauI CCCGC 2 cut(s) 58, 597
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 9 cut(s) 28, 50, 60, 63, 165, 311, 356, 369, 541
Fsp4HI GCNGC 9 cut(s) 28, 50, 60, 63, 165, 311, 356, 369, 541
FspBI CTAG 1 cut(s) 114
GlaI GCGC 2 cut(s) 31, 76
GluI GCNGC 9 cut(s) 28, 50, 60, 63, 165, 311, 356, 369, 541
GsaI CCCAGC 1 cut(s) 241
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 1 cut(s) 235
HapII CCGG 4 cut(s) 140, 210, 382, 438
HhaI GCGC 2 cut(s) 32, 77
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 389, 403, 540
Hin6I GCGC 2 cut(s) 30, 75
HinP1I GCGC 2 cut(s) 30, 75
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 39, 90
HpaII CCGG 4 cut(s) 140, 210, 382, 438
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 2 cut(s) 496, 505
Hpy188I TCNGA 2 cut(s) 488, 585
Hpy188III TCNNGA 1 cut(s) 109
Hpy8I GTNNAC 2 cut(s) 496, 505
Hpy99I CGWCG 1 cut(s) 262
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 4 cut(s) 262, 270, 422, 527
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 225, 473
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 170, 222, 368, 455
HpyF3I CTNAG 1 cut(s) 514
HpySE526I ACGT 1 cut(s) 426
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 389, 403, 540
HspAI GCGC 2 cut(s) 30, 75
KasI GGCGCC 1 cut(s) 74
Kzo9I GATC 1 cut(s) 585
Lsp1109I GCAGC 6 cut(s) 14, 36, 49, 151, 297, 342
MaeI CTAG 1 cut(s) 114
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 157, 422
MalI GATC 1 cut(s) 587
MboI GATC 1 cut(s) 585
MfeI CAATTG 1 cut(s) 217
MflI RGATCY 1 cut(s) 585
MhlI GDGCHC 2 cut(s) 86, 460
MluCI AATT 3 cut(s) 192, 217, 489
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 1 cut(s) 33
MmeI TCCRAC 1 cut(s) 270
MnlI CCTC 4 cut(s) 225, 446, 470, 591
MseI TTAA 2 cut(s) 99, 509
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 4 cut(s) 140, 210, 382, 438
MspR9I CCNGG 3 cut(s) 44, 211, 438
MunI CAATTG 1 cut(s) 217
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 5 cut(s) 81, 170, 222, 368, 455
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 2 cut(s) 211, 438
NdeII GATC 1 cut(s) 585
NlaIII CATG 3 cut(s) 389, 403, 540
NlaIV GGNNCC 5 cut(s) 76, 236, 367, 457, 587
NmeAIII GCCGAG 1 cut(s) 103
NspI RCATGY 1 cut(s) 403
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PfeI GAWTC 1 cut(s) 90
PkrI GCNGC 9 cut(s) 29, 51, 61, 64, 166, 312, 357, 370, 542
PleI GAGTC 1 cut(s) 33
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 33
PsiI TTATAA 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 5 cut(s) 76, 236, 367, 457, 587
PspPI GGNCC 1 cut(s) 234
PsuI RGATCY 1 cut(s) 585
RsaI GTAC 2 cut(s) 404, 483
RsaNI GTAC 2 cut(s) 403, 482
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 2 cut(s) 99, 509
SatI GCNGC 9 cut(s) 28, 50, 60, 63, 165, 311, 356, 369, 541
Sau3AI GATC 1 cut(s) 585
Sau96I GGNCC 1 cut(s) 234
SchI GAGTC 1 cut(s) 33
ScrFI CCNGG 3 cut(s) 44, 211, 438
SduI GDGCHC 2 cut(s) 86, 460
SetI ASST 6 cut(s) 99, 187, 429, 457, 571, 583
SfoI GGCGCC 1 cut(s) 76
SmiMI CAYNNNNRTG 1 cut(s) 384
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 3 cut(s) 192, 217, 489
SsiI CCGC 8 cut(s) 59, 65, 167, 369, 479, 540, 543, 590
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 1 cut(s) 114
StyD4I CCNGG 3 cut(s) 42, 209, 436
TaaI ACNGT 4 cut(s) 262, 270, 422, 527
TaiI ACGT 1 cut(s) 429
TaqI TCGA 2 cut(s) 110, 257
TasI AATT 3 cut(s) 192, 217, 489
TauI GCSGC 3 cut(s) 62, 371, 543
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 2 cut(s) 99, 509
Tru9I TTAA 2 cut(s) 99, 509
TscAI CASTG 1 cut(s) 532
TseI GCWGC 6 cut(s) 27, 49, 62, 164, 310, 355
TspRI CASTG 1 cut(s) 532
XceI RCATGY 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 495
XspI CTAG 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.