MD02G1004300.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0028573
Physical Location & Seq
Forward (+)
295111 .. 296795
1685 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1004300.v1.1.491

Sequence Viewer

Length: 954 bp
ATGTGTAAGCAATCGGAGAACGGGATTCCGGTGATCGATTTCTCCAAGGAGGAGGCGTTGAAGCCGGGGTCGAGTTCTTGGCTAGGGATACGTGAAGATGTTATCCGTGCACTCGAAGGAACGGGCTGCTTCATTGCATTAGTTGGCAACAAGGTCCGCCAAGAGCTTCGCAGCACCATGTTGGAGGCTTTCAAAGAGTTGATGGATTTCCCCCGTGAAGCCAAGATGAAGGTCAAGAGCAAGAACAAGAAGACCTTGGGTGCATATCACAACACCACTCAGATCCACGAAAGCCTGGCAATGGACGACGCCGCAGATGCTGCTGAGTGCCAGAAGTTCAGCAATCTCTTCTGGCCCAACGGAAATGAAAATTTCACTCGAAGTTCGAACGAGTATGCAACGCAAATGATGAACTTAGACTGGATAGTGACGAGGATGATATTCGAAAGCTATGGTGTTGACAACTACCATGATACACATATTAACACCAACACTCACCACATGTCGTTCCTCAAGTACGAGCGATCGGAGGAAATTGAAACCGACGTGGGTGTTTTTATTCACACCGACAAGAACTTCACCACGGTGCTGCATCAGGTGAACGACAGCCATGGCTTGGAGGTGCAGAACAAGAACGGGGACTGGGTGCTCTGTCAACCACCGCCATCGTCCTTCACTTTCATCGCAGGCGATATTTTGCAGGTTTGGAGCAATGATAAAATACAAGCTTGCAAACACAGAGTGACAATAAGCAAAGACACGCAAAGGTTCGTTTTGGCTCTGTTTCTGTTCAAGAGTGGCAAGTTCCAGACGCCGCAAGAGTTGATTGACGAGGAGCATCCCGTCCGCTACAAACCGATTGATCAATCTGAATATTTTGCCGCCGTTATGAACAACGCAATAAATGGAGGAATTAGTTACACTGTCAAAGACCACTGTGGCATTACAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

318

Amino Acids

36.13

Weight (kDa)

5.75

Isoelectric Point (pI)

33.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 9 - 109 1.3e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 168 - 264 1.4e-17 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028573)

Species Orthologous Gene IDs
malus_domestica MD02G1004300.v1.1
pyrus_communis pycom02g00280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 691
AccB7I CCANNNNNTGG 1 cut(s) 616
AciI CCGC 6 cut(s) 157, 312, 662, 815, 847, 882
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 370
AcyI GRCGYC 2 cut(s) 309, 812
AdeI CACNNNGTG 1 cut(s) 742
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 2 cut(s) 301, 616
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 4 cut(s) 61, 193, 539, 793
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 1 cut(s) 294
AleI CACNNNNGTG 1 cut(s) 584
AluBI AGCT 3 cut(s) 166, 450, 728
AluI AGCT 3 cut(s) 166, 450, 728
Alw21I GWGCWC 2 cut(s) 112, 651
Alw44I GTGCAC 1 cut(s) 108
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 1 cut(s) 353
ApaLI GTGCAC 1 cut(s) 108
ApeKI GCWGC 4 cut(s) 126, 171, 320, 589
ApoI RAATTY 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 154, 354
AsuC2I CCSGG 1 cut(s) 66
AsuHPI GGTGA 4 cut(s) 43, 488, 571, 610
AsuII TTCGAA 2 cut(s) 386, 444
AvaII GGWCC 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 112
BbsI GAAGAC 1 cut(s) 257
Bbv12I GWGCWC 2 cut(s) 112, 651
BbvI GCAGC 4 cut(s) 113, 183, 307, 576
BccI CCATC 2 cut(s) 196, 673
BceAI ACGGC 1 cut(s) 869
BciT130I CCWGG 1 cut(s) 296
BciVI GTATCC 1 cut(s) 81
BclI TGATCA 1 cut(s) 862
BcnI CCSGG 1 cut(s) 66
BfaI CTAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 691
BfuI GTATCC 1 cut(s) 81
BisI GCNGC 7 cut(s) 127, 172, 312, 321, 590, 815, 882
BlsI GCNGC 7 cut(s) 128, 173, 313, 322, 591, 816, 883
Bme1390I CCNGG 2 cut(s) 66, 296
Bme18I GGWCC 1 cut(s) 154
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 2 cut(s) 154, 354
BmrFI CCNGG 2 cut(s) 66, 296
BmrI ACTGGG 1 cut(s) 652
BmsI GCATC 3 cut(s) 307, 601, 847
BmuI ACTGGG 1 cut(s) 652
BpiI GAAGAC 1 cut(s) 257
Bpu14I TTCGAA 2 cut(s) 386, 444
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 66
Bsa29I ATCGAT 1 cut(s) 36
BsaAI YACGTR 1 cut(s) 92
BsaHI GRCGYC 2 cut(s) 309, 812
BsaJI CCNNGG 5 cut(s) 45, 65, 255, 582, 610
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 616
Bse1I ACTGG 2 cut(s) 425, 647
Bse3DI GCAATG 3 cut(s) 132, 306, 718
BseBI CCWGG 1 cut(s) 296
BseCI ATCGAT 1 cut(s) 36
BseDI CCNNGG 5 cut(s) 45, 65, 255, 582, 610
BseGI GGATG 2 cut(s) 441, 838
BseLI CCNNNNNNNGG 2 cut(s) 301, 616
BseMI GCAATG 3 cut(s) 132, 306, 718
BseMII CTCAG 2 cut(s) 293, 315
BseNI ACTGG 2 cut(s) 425, 647
BseRI GAGGAG 2 cut(s) 65, 848
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 4 cut(s) 113, 183, 307, 576
BsgI GTGCAG 1 cut(s) 644
Bsh1285I CGRYCG 1 cut(s) 527
BshFI GGCC 1 cut(s) 355
BshVI ATCGAT 1 cut(s) 36
BsiEI CGRYCG 1 cut(s) 527
BsiHKAI GWGCWC 2 cut(s) 112, 651
BsiSI CCGG 2 cut(s) 29, 65
BslFI GGGAC 1 cut(s) 653
BslI CCNNNNNNNGG 2 cut(s) 301, 616
BsmFI GGGAC 1 cut(s) 653
BsnI GGCC 1 cut(s) 355
Bsp119I TTCGAA 2 cut(s) 386, 444
Bsp1286I GDGCHC 2 cut(s) 112, 651
Bsp143I GATC 4 cut(s) 33, 282, 524, 862
Bsp19I CCATGG 1 cut(s) 610
BspACI CCGC 6 cut(s) 157, 312, 662, 815, 847, 882
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 2 cut(s) 292, 316
BspDI ATCGAT 1 cut(s) 36
BspMI ACCTGC 1 cut(s) 691
BspPI GGATC 1 cut(s) 277
BspT104I TTCGAA 2 cut(s) 386, 444
BsrDI GCAATG 3 cut(s) 132, 306, 718
BsrI ACTGG 2 cut(s) 425, 647
BssECI CCNNGG 5 cut(s) 45, 65, 255, 582, 610
BssMI GATC 4 cut(s) 33, 282, 524, 862
BssNI GRCGYC 2 cut(s) 309, 812
BssT1I CCWWGG 3 cut(s) 45, 255, 610
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 586, 925, 938, 949
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 2 cut(s) 309, 812
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 92
BstBI TTCGAA 2 cut(s) 386, 444
BstC8I GCNNGC 2 cut(s) 688, 730
BstDEI CTNAG 3 cut(s) 279, 324, 415
BstDSI CCRYGG 2 cut(s) 582, 610
BstF5I GGATG 2 cut(s) 441, 838
BstKTI GATC 4 cut(s) 36, 285, 527, 865
BstMBI GATC 4 cut(s) 33, 282, 524, 862
BstMCI CGRYCG 1 cut(s) 527
BstMWI GCNNNNNNNGC 2 cut(s) 317, 320
BstNI CCWGG 1 cut(s) 296
BstNSI RCATGY 1 cut(s) 505
BstSCI CCNGG 2 cut(s) 64, 294
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 4 cut(s) 113, 183, 307, 576
BstV2I GAAGAC 1 cut(s) 257
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
Bsu15I ATCGAT 1 cut(s) 36
BsuI GTATCC 1 cut(s) 81
BsuRI GGCC 1 cut(s) 355
BsuTUI ATCGAT 1 cut(s) 36
BtgI CCRYGG 2 cut(s) 582, 610
BtgZI GCGATG 1 cut(s) 667
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 2 cut(s) 441, 838
BtsIMutI CAGTG 2 cut(s) 921, 934
BveI ACCTGC 1 cut(s) 691
Cac8I GCNNGC 2 cut(s) 688, 730
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 154, 354
ClaI ATCGAT 1 cut(s) 36
CseI GACGC 2 cut(s) 317, 820
Csp6I GTAC 1 cut(s) 517
CviAII CATG 4 cut(s) 178, 470, 502, 611
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 3 cut(s) 279, 324, 415
DpnI GATC 4 cut(s) 35, 284, 526, 864
DpnII GATC 4 cut(s) 33, 282, 524, 862
DraIII CACNNNGTG 1 cut(s) 742
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 1 cut(s) 146
Eco130I CCWWGG 3 cut(s) 45, 255, 610
Eco47I GGWCC 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 3 cut(s) 45, 255, 610
ErhI CCWWGG 3 cut(s) 45, 255, 610
FaeI CATG 4 cut(s) 181, 473, 505, 614
FaiI YATR 9 cut(s) 179, 265, 396, 453, 471, 480, 503, 612, 890
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FaqI GGGAC 1 cut(s) 653
FatI CATG 4 cut(s) 177, 469, 501, 610
FbaI TGATCA 1 cut(s) 862
Fnu4HI GCNGC 7 cut(s) 127, 172, 312, 321, 590, 815, 882
FokI GGATG 2 cut(s) 448, 825
Fsp4HI GCNGC 7 cut(s) 127, 172, 312, 321, 590, 815, 882
FspBI CTAG 1 cut(s) 83
GluI GCNGC 7 cut(s) 127, 172, 312, 321, 590, 815, 882
HaeIII GGCC 1 cut(s) 355
HapII CCGG 2 cut(s) 29, 65
HgaI GACGC 2 cut(s) 317, 820
Hin1I GRCGYC 2 cut(s) 309, 812
Hin1II CATG 4 cut(s) 181, 473, 505, 614
HincII GTYRAC 2 cut(s) 460, 656
HindII GTYRAC 2 cut(s) 460, 656
HindIII AAGCTT 1 cut(s) 726
HinfI GANTC 1 cut(s) 25
HpaII CCGG 2 cut(s) 29, 65
HphI GGTGA 4 cut(s) 43, 488, 571, 610
Hpy166II GTNNAC 4 cut(s) 110, 460, 601, 656
Hpy188I TCNGA 4 cut(s) 16, 282, 529, 871
Hpy188III TCNNGA 3 cut(s) 235, 793, 808
Hpy8I GTNNAC 4 cut(s) 110, 460, 601, 656
Hpy99I CGWCG 2 cut(s) 311, 548
HpyAV CCTTC 3 cut(s) 110, 223, 682
HpyCH4III ACNGT 4 cut(s) 586, 925, 938, 949
HpyCH4IV ACGT 2 cut(s) 91, 546
HpyCH4V TGCA 8 cut(s) 110, 137, 263, 398, 592, 625, 700, 732
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 320
HpyF3I CTNAG 3 cut(s) 279, 324, 415
HpySE526I ACGT 2 cut(s) 91, 546
Hsp92I GRCGYC 2 cut(s) 309, 812
Hsp92II CATG 4 cut(s) 181, 473, 505, 614
Ksp22I TGATCA 1 cut(s) 862
Kzo9I GATC 4 cut(s) 33, 282, 524, 862
LmnI GCTCC 2 cut(s) 708, 835
Lsp1109I GCAGC 4 cut(s) 113, 183, 307, 576
LweI GCATC 3 cut(s) 307, 601, 847
MaeI CTAG 1 cut(s) 83
MaeII ACGT 2 cut(s) 91, 546
MaeIII GTNAC 3 cut(s) 427, 742, 917
MalI GATC 4 cut(s) 35, 284, 526, 864
MboI GATC 4 cut(s) 33, 282, 524, 862
MboII GAAGA 3 cut(s) 107, 262, 340
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 2 cut(s) 112, 651
MluCI AATT 3 cut(s) 370, 534, 912
MmeI TCCRAC 1 cut(s) 162
MnlI CCTC 9 cut(s) 43, 46, 178, 426, 521, 523, 613, 826, 902
MseI TTAA 1 cut(s) 483
MslI CAYNNNNRTG 1 cut(s) 584
MspI CCGG 2 cut(s) 29, 65
MspR9I CCNGG 2 cut(s) 66, 296
MvaI CCWGG 1 cut(s) 296
MwoI GCNNNNNNNGC 2 cut(s) 317, 320
NciI CCSGG 1 cut(s) 66
NcoI CCATGG 1 cut(s) 610
NdeII GATC 4 cut(s) 33, 282, 524, 862
NlaIII CATG 4 cut(s) 181, 473, 505, 614
NmuCI GTSAC 2 cut(s) 427, 742
NspI RCATGY 1 cut(s) 505
NspV TTCGAA 2 cut(s) 386, 444
OliI CACNNNNGTG 1 cut(s) 584
PciI ACATGT 1 cut(s) 501
PfeI GAWTC 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 616
PkrI GCNGC 7 cut(s) 128, 173, 313, 322, 591, 816, 883
Ple19I CGATCG 1 cut(s) 527
Ppu21I YACGTR 1 cut(s) 92
PscI ACATGT 1 cut(s) 501
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspPI GGNCC 2 cut(s) 154, 354
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 282
PvuI CGATCG 1 cut(s) 527
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 1 cut(s) 584
SaqAI TTAA 1 cut(s) 483
SatI GCNGC 7 cut(s) 127, 172, 312, 321, 590, 815, 882
Sau3AI GATC 4 cut(s) 33, 282, 524, 862
Sau96I GGNCC 2 cut(s) 154, 354
ScrFI CCNGG 2 cut(s) 66, 296
SduI GDGCHC 2 cut(s) 112, 651
SfaNI GCATC 3 cut(s) 307, 601, 847
SfuI TTCGAA 2 cut(s) 386, 444
SinI GGWCC 1 cut(s) 154
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
Sse9I AATT 3 cut(s) 370, 534, 912
SsiI CCGC 6 cut(s) 157, 312, 662, 815, 847, 882
SspI AATATT 1 cut(s) 875
SspMI CTAG 1 cut(s) 83
StyD4I CCNGG 2 cut(s) 64, 294
StyI CCWWGG 3 cut(s) 45, 255, 610
TaaI ACNGT 4 cut(s) 586, 925, 938, 949
TaiI ACGT 2 cut(s) 94, 549
TaqI TCGA 6 cut(s) 36, 71, 114, 379, 386, 444
TasI AATT 3 cut(s) 370, 534, 912
TauI GCSGC 3 cut(s) 314, 817, 884
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 1 cut(s) 483
Tru9I TTAA 1 cut(s) 483
TscAI CASTG 2 cut(s) 928, 941
TseFI GTSAC 2 cut(s) 427, 742
TseI GCWGC 4 cut(s) 126, 171, 320, 589
Tsp45I GTSAC 2 cut(s) 427, 742
TspDTI ATGAA 6 cut(s) 121, 242, 381, 425, 670, 905
TspGWI ACGGA 2 cut(s) 95, 375
TspRI CASTG 2 cut(s) 928, 941
Van91I CCANNNNNTGG 1 cut(s) 616
VneI GTGCAC 1 cut(s) 108
VpaK11BI GGWCC 1 cut(s) 154
XapI RAATTY 1 cut(s) 370
XceI RCATGY 1 cut(s) 505
XspI CTAG 1 cut(s) 83
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.