MD02G1016800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0028598
Physical Location & Seq
Forward (+)
1258181 .. 1260777
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1016800.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGTGTTTGTTTACGGTTTGTGCTCGTGTTGGCATTGTAGGGGATGGTGGTGATCAGAGAGAGCATGGTGGGGTGGTGGTGACTTGGGGCACAAGAAGGTCTCTAGCAGAAGAAAAAACTCCCCGAAACGCACCTCTGATGATATTGTCTAGGGAGAGGACACGCCGGAAAGACCCTCGTGACGGATTTAAGAGATACACCGGTGGCTGGAACATCAGCAATGAACATTACTGGGCTTCTGTTAACTATACCGCGACGCCCTTCCTTGTCATTGCTGTCGTATGGTTCAAGATTTTCGGGATAAGCTTGTGCCTAACCTGTATCTGCAACTTCTGTCGCCCTAAAGAGCCTTATGGTTATTCTAGAAGAGCTTATGCTCTCTCTCCCATCTTCCTCGTTTTCTTCACCCTTGCCGCAATCATTGGATTCATTGTTTTGTTCTCCGGTCAAGGAAAGTTCCAGGGAAGTTCAACCGATGCACTGAACCATGTTGTTAGTCAGGCAAATACAACTGCTGCAAAGCTCAGGAATGTGTCATATAATCTCAGTGCAGCTAAGCGGATTGGAGTTGATGCTGTGTTTCTGCCAGAAGATGTCCAGAAGAAAATTGACAATATCGTAACAAAAATTGACGCTTCTGCAGCTACACTTTCTGATAGAACAAAAAAGAATTTAAAGACTATAAAAGATAGTTTAAATACCATCTTGGTGATTGTCGGGTGGATTCTTGTCACGTTAGCGTTCATATTGTGCGGTGCATTTGTTGTCCTCCACAATGCGATTGGAGATGCATGTGTCGCCATGGATGAATGGGTTCAGAACCCTAAACCTCATACTGCATTTGACGATATACTCCCATGTGTGGACCATGCTACTGCCCAGGAGGCTGCGTTGCGCACCAAGGATGCAACCTTCCAGCTTGTTACCGTGGTTAACCATGTCATAACCAATGCTTCGAATGTCGACAATCAGTTAGACAGACCTGTACCTCTTCTTTGCAACCCTTTCCATTCAAATCTCATCGACCGACATTGTATCTCCGGTGAAGTGCCACTGCAAAATGCCACGCAGGTATGGAAGAAATATGTGTGTGAGGTTTCAAAGGCTGGTACTTGTACAAAACCGGGCAGCTGGCTGACTCCCACATTGTACGGACAAATGGAAACTACTGCAAATCTGAGCTACGTACTCTACGGTTACGTTAAATTCTTGACGAATCTGCAATCCTGCAGATCTGTTAGGGATGCATTCAGAGACATAAGCAGTGGGAAGTGTCATGGTTTGGAAAAACACAGCAGCTGGATTTACATTGGTTTGGCAATGGTGTCTGCAGCTCTGGTGCTTTCAATGATCTTCTGGGTTATCTACGCGAGGGAACGAAGTCATCGTCTTTATATCGAAAACTGCTTTCTGGAGAGAGCCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

476

Amino Acids

52.89

Weight (kDa)

9.03

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028598)

Species Orthologous Gene IDs
malus_domestica MD02G1016800.v1.1
pyrus_communis pycom02g01510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 896
Acc36I ACCTGC 1 cut(s) 1060
AccI GTMKAC 1 cut(s) 963
AccII CGCG 2 cut(s) 254, 1370
AciI CCGC 4 cut(s) 252, 414, 559, 753
AcsI RAATTY 2 cut(s) 670, 1205
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 5 cut(s) 987, 1111, 1117, 1151, 1188
AfiI CCNNNNNNNGG 3 cut(s) 182, 207, 862
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 5 cut(s) 289, 471, 1014, 1101, 1347
AjnI CCWGG 2 cut(s) 459, 879
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 2 cut(s) 105, 1248
AlwNI CAGNNNCTG 1 cut(s) 1299
ApeKI GCWGC 7 cut(s) 515, 551, 641, 887, 1128, 1296, 1331
ApoI RAATTY 2 cut(s) 670, 1205
ArsI GACNNNNNNTTYG 2 cut(s) 1372, 1404
AsiGI ACCGGT 1 cut(s) 200
Asp700I GAANNNNTTC 1 cut(s) 813
AspLEI GCGC 1 cut(s) 897
AspS9I GGNCC 1 cut(s) 865
AsuC2I CCSGG 1 cut(s) 1125
AsuHPI GGTGA 5 cut(s) 62, 91, 397, 721, 1055
AsuII TTCGAA 1 cut(s) 956
AvaII GGWCC 1 cut(s) 865
BaeGI GKGCMC 1 cut(s) 92
BaeI ACNNNNGTAYC 2 cut(s) 187, 220
BauI CACGAG 2 cut(s) 24, 177
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 7 cut(s) 502, 563, 653, 874, 1140, 1308, 1343
BccI CCATC 3 cut(s) 38, 395, 710
BciT130I CCWGG 2 cut(s) 461, 881
BclI TGATCA 1 cut(s) 52
BcnI CCSGG 1 cut(s) 1125
BcoDI GTCTC 2 cut(s) 105, 1248
BfaI CTAG 3 cut(s) 104, 150, 363
BfmI CTRYAG 3 cut(s) 639, 1228, 1329
BfuAI ACCTGC 1 cut(s) 1060
BglI GCCNNNNNGGC 1 cut(s) 884
BglII AGATCT 1 cut(s) 1232
BisI GCNGC 8 cut(s) 414, 516, 552, 642, 888, 1129, 1297, 1332
BlpI GCTNAGC 1 cut(s) 555
BlsI GCNGC 8 cut(s) 415, 517, 553, 643, 889, 1130, 1298, 1333
Bme1390I CCNGG 3 cut(s) 461, 881, 1125
Bme18I GGWCC 1 cut(s) 865
BmgT120I GGNCC 1 cut(s) 865
BmrFI CCNGG 3 cut(s) 461, 881, 1125
BmrI ACTGGG 1 cut(s) 241
BmsI GCATC 5 cut(s) 466, 562, 778, 895, 1234
BmuI ACTGGG 1 cut(s) 241
Bpu10I CCTNAGC 1 cut(s) 524
Bpu1102I GCTNAGC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 956
BpuMI CCSGG 1 cut(s) 1125
BsaAI YACGTR 1 cut(s) 1186
BsaHI GRCGYC 1 cut(s) 257
BsaI GGTCTC 1 cut(s) 105
BsaJI CCNNGG 5 cut(s) 460, 801, 879, 900, 927
BsaWI WCCGGW 3 cut(s) 200, 443, 1040
BsaXI ACNNNNNCTCC 2 cut(s) 875, 905
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 207, 862
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 3 cut(s) 226, 270, 1326
BseBI CCWGG 2 cut(s) 461, 881
BseDI CCNNGG 5 cut(s) 460, 801, 879, 900, 927
BseGI GGATG 4 cut(s) 49, 811, 910, 1249
BseLI CCNNNNNNNGG 3 cut(s) 182, 207, 862
BseMI GCAATG 3 cut(s) 226, 270, 1326
BseMII CTCAG 3 cut(s) 538, 559, 1169
BseNI ACTGG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 92
BseXI GCAGC 7 cut(s) 502, 563, 653, 874, 1140, 1308, 1343
BsgI GTGCAG 1 cut(s) 570
Bsh1236I CGCG 2 cut(s) 254, 1370
Bsh1285I CGRYCG 1 cut(s) 1027
BshTI ACCGGT 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 1027
BsiHKAI GWGCWC 1 cut(s) 25
BsiSI CCGG 5 cut(s) 166, 201, 444, 1041, 1124
BslI CCNNNNNNNGG 3 cut(s) 182, 207, 862
BsmAI GTCTC 2 cut(s) 105, 1248
BsmI GAATGC 1 cut(s) 1247
Bso31I GGTCTC 1 cut(s) 105
Bsp119I TTCGAA 1 cut(s) 956
Bsp1286I GDGCHC 2 cut(s) 25, 92
Bsp1407I TGTACA 1 cut(s) 1115
Bsp143I GATC 3 cut(s) 52, 1232, 1350
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 1 cut(s) 801
BspACI CCGC 4 cut(s) 252, 414, 559, 753
BspCNI CTCAG 3 cut(s) 537, 558, 1170
BspFNI CGCG 2 cut(s) 254, 1370
BspMAI CTGCAG 3 cut(s) 643, 1232, 1333
BspMI ACCTGC 1 cut(s) 1060
BspQI GCTCTTC 1 cut(s) 361
BspT104I TTCGAA 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 105
BsrDI GCAATG 3 cut(s) 226, 270, 1326
BsrFI RCCGGY 1 cut(s) 200
BsrGI TGTACA 1 cut(s) 1115
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 460, 801, 879, 900, 927
BssMI GATC 3 cut(s) 52, 1232, 1350
BssNI GRCGYC 1 cut(s) 257
BssSI CACGAG 2 cut(s) 24, 177
BssT1I CCWWGG 2 cut(s) 801, 900
Bst2BI CACGAG 2 cut(s) 24, 177
Bst2UI CCWGG 2 cut(s) 461, 881
Bst4CI ACNGT 3 cut(s) 16, 928, 1196
Bst6I CTCTTC 2 cut(s) 361, 996
BstACI GRCGYC 1 cut(s) 257
BstAUI TGTACA 1 cut(s) 1115
BstBAI YACGTR 1 cut(s) 1186
BstBI TTCGAA 1 cut(s) 956
BstC8I GCNNGC 1 cut(s) 1133
BstDEI CTNAG 4 cut(s) 524, 545, 555, 1178
BstDSI CCRYGG 2 cut(s) 801, 927
BstF5I GGATG 4 cut(s) 49, 811, 910, 1249
BstFNI CGCG 2 cut(s) 254, 1370
BstHHI GCGC 1 cut(s) 897
BstKTI GATC 3 cut(s) 55, 1235, 1353
BstMAI GTCTC 2 cut(s) 105, 1248
BstMBI GATC 3 cut(s) 52, 1232, 1350
BstMCI CGRYCG 1 cut(s) 1027
BstMWI GCNNNNNNNGC 3 cut(s) 641, 797, 884
BstNI CCWGG 2 cut(s) 461, 881
BstNSI RCATGY 1 cut(s) 795
BstSCI CCNGG 3 cut(s) 459, 879, 1123
BstSFI CTRYAG 3 cut(s) 639, 1228, 1329
BstSLI GKGCMC 1 cut(s) 92
BstSNI TACGTA 1 cut(s) 1186
BstUI CGCG 2 cut(s) 254, 1370
BstV1I GCAGC 7 cut(s) 502, 563, 653, 874, 1140, 1308, 1343
BstX2I RGATCY 1 cut(s) 1232
BstYI RGATCY 1 cut(s) 1232
BtgI CCRYGG 2 cut(s) 801, 927
BtsCI GGATG 4 cut(s) 49, 811, 910, 1249
BtsI GCAGTG 2 cut(s) 1052, 1270
BtsIMutI CAGTG 4 cut(s) 479, 553, 1052, 1270
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 1 cut(s) 1133
CaiI CAGNNNCTG 1 cut(s) 1299
CfoI GCGC 1 cut(s) 897
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 865
CseI GACGC 2 cut(s) 265, 641
Csp6I GTAC 5 cut(s) 986, 1110, 1116, 1150, 1187
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 8 cut(s) 65, 488, 792, 802, 858, 869, 938, 1277
CviQI GTAC 5 cut(s) 986, 1110, 1116, 1150, 1187
DdeI CTNAG 4 cut(s) 524, 545, 555, 1178
DpnI GATC 3 cut(s) 54, 1234, 1352
DpnII GATC 3 cut(s) 52, 1232, 1350
DraI TTTAAA 2 cut(s) 675, 696
Eam1104I CTCTTC 2 cut(s) 361, 996
EarI CTCTTC 2 cut(s) 361, 996
Eco105I TACGTA 1 cut(s) 1186
Eco130I CCWWGG 2 cut(s) 801, 900
Eco31I GGTCTC 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 865
EcoRII CCWGG 2 cut(s) 459, 879
EcoT14I CCWWGG 2 cut(s) 801, 900
EcoT22I ATGCAT 2 cut(s) 793, 1249
ErhI CCWWGG 2 cut(s) 801, 900
FaeI CATG 8 cut(s) 68, 491, 795, 805, 861, 872, 941, 1280
FatI CATG 8 cut(s) 64, 487, 791, 801, 857, 868, 937, 1276
FbaI TGATCA 1 cut(s) 52
FblI GTMKAC 1 cut(s) 963
Fnu4HI GCNGC 8 cut(s) 414, 516, 552, 642, 888, 1129, 1297, 1332
FokI GGATG 4 cut(s) 56, 818, 917, 1256
Fsp4HI GCNGC 8 cut(s) 414, 516, 552, 642, 888, 1129, 1297, 1332
FspBI CTAG 3 cut(s) 104, 150, 363
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 1 cut(s) 896
GluI GCNGC 8 cut(s) 414, 516, 552, 642, 888, 1129, 1297, 1332
HapII CCGG 5 cut(s) 166, 201, 444, 1041, 1124
HgaI GACGC 2 cut(s) 265, 641
HhaI GCGC 1 cut(s) 897
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 8 cut(s) 68, 491, 795, 805, 861, 872, 941, 1280
Hin6I GCGC 1 cut(s) 895
HinP1I GCGC 1 cut(s) 895
HincII GTYRAC 3 cut(s) 244, 934, 964
HindII GTYRAC 3 cut(s) 244, 934, 964
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 4 cut(s) 426, 724, 1138, 1216
HpaI GTTAAC 2 cut(s) 244, 934
HpaII CCGG 5 cut(s) 166, 201, 444, 1041, 1124
HphI GGTGA 5 cut(s) 62, 91, 397, 721, 1055
Hpy166II GTNNAC 5 cut(s) 12, 244, 865, 934, 964
Hpy188I TCNGA 6 cut(s) 57, 138, 655, 819, 1179, 1253
Hpy188III TCNNGA 8 cut(s) 179, 289, 298, 363, 526, 598, 1210, 1412
Hpy8I GTNNAC 5 cut(s) 12, 244, 865, 934, 964
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 3 cut(s) 90, 271, 922
HpyCH4III ACNGT 3 cut(s) 16, 928, 1196
HpyCH4IV ACGT 3 cut(s) 734, 1185, 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 641, 797, 884
HpyF3I CTNAG 4 cut(s) 524, 545, 555, 1178
HpySE526I ACGT 3 cut(s) 734, 1185, 1200
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 8 cut(s) 68, 491, 795, 805, 861, 872, 941, 1280
HspAI GCGC 1 cut(s) 895
Ksp22I TGATCA 1 cut(s) 52
KspAI GTTAAC 2 cut(s) 244, 934
Kzo9I GATC 3 cut(s) 52, 1232, 1350
LguI GCTCTTC 1 cut(s) 361
Lsp1109I GCAGC 7 cut(s) 502, 563, 653, 874, 1140, 1308, 1343
LweI GCATC 5 cut(s) 466, 562, 778, 895, 1234
MaeI CTAG 3 cut(s) 104, 150, 363
MaeII ACGT 3 cut(s) 734, 1185, 1200
MaeIII GTNAC 6 cut(s) 79, 179, 619, 730, 922, 1196
MalI GATC 3 cut(s) 54, 1234, 1352
MboI GATC 3 cut(s) 52, 1232, 1350
MboII GAAGA 9 cut(s) 122, 378, 382, 394, 602, 613, 983, 1090, 1345
MflI RGATCY 1 cut(s) 1232
MhlI GDGCHC 2 cut(s) 25, 92
MluCI AATT 4 cut(s) 606, 627, 670, 1205
MlyI GAGTC 1 cut(s) 1132
Mph1103I ATGCAT 2 cut(s) 793, 1249
MroXI GAANNNNTTC 1 cut(s) 813
MseI TTAA 6 cut(s) 189, 243, 674, 695, 933, 1203
MslI CAYNNNNRTG 1 cut(s) 707
MspA1I CMGCKG 2 cut(s) 1131, 1299
MspI CCGG 5 cut(s) 166, 201, 444, 1041, 1124
MspR9I CCNGG 3 cut(s) 461, 881, 1125
Mva1269I GAATGC 1 cut(s) 1247
MvaI CCWGG 2 cut(s) 461, 881
MvnI CGCG 2 cut(s) 254, 1370
MwoI GCNNNNNNNGC 3 cut(s) 641, 797, 884
NciI CCSGG 1 cut(s) 1125
NcoI CCATGG 1 cut(s) 801
NdeII GATC 3 cut(s) 52, 1232, 1350
NlaIII CATG 8 cut(s) 68, 491, 795, 805, 861, 872, 941, 1280
NmuCI GTSAC 3 cut(s) 79, 179, 730
NsbI TGCGCA 1 cut(s) 896
NsiI ATGCAT 2 cut(s) 793, 1249
NspI RCATGY 1 cut(s) 795
NspV TTCGAA 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 1384
PctI GAATGC 1 cut(s) 1247
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 3 cut(s) 426, 724, 1216
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 8 cut(s) 415, 517, 553, 643, 889, 1130, 1298, 1333
PleI GAGTC 1 cut(s) 1132
PpsI GAGTC 1 cut(s) 1132
Ppu21I YACGTR 1 cut(s) 1186
Psp6I CCWGG 2 cut(s) 459, 879
PspGI CCWGG 2 cut(s) 459, 879
PspPI GGNCC 1 cut(s) 865
PstI CTGCAG 3 cut(s) 643, 1232, 1333
PstNI CAGNNNCTG 1 cut(s) 1299
PsuI RGATCY 1 cut(s) 1232
PvuII CAGCTG 2 cut(s) 1131, 1299
RsaI GTAC 5 cut(s) 987, 1111, 1117, 1151, 1188
RsaNI GTAC 5 cut(s) 986, 1110, 1116, 1150, 1187
RseI CAYNNNNRTG 1 cut(s) 707
SalI GTCGAC 1 cut(s) 962
SapI GCTCTTC 1 cut(s) 361
SaqAI TTAA 6 cut(s) 189, 243, 674, 695, 933, 1203
SatI GCNGC 8 cut(s) 414, 516, 552, 642, 888, 1129, 1297, 1332
Sau3AI GATC 3 cut(s) 52, 1232, 1350
Sau96I GGNCC 1 cut(s) 865
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 3 cut(s) 461, 881, 1125
SduI GDGCHC 2 cut(s) 25, 92
SfaNI GCATC 5 cut(s) 466, 562, 778, 895, 1234
SfcI CTRYAG 3 cut(s) 639, 1228, 1329
SfuI TTCGAA 1 cut(s) 956
SgrAI CRCCGGYG 1 cut(s) 200
SinI GGWCC 1 cut(s) 865
SmiMI CAYNNNNRTG 1 cut(s) 707
SnaBI TACGTA 1 cut(s) 1186
Sse9I AATT 4 cut(s) 606, 627, 670, 1205
SsiI CCGC 4 cut(s) 252, 414, 559, 753
SspMI CTAG 3 cut(s) 104, 150, 363
StyD4I CCNGG 3 cut(s) 459, 879, 1123
StyI CCWWGG 2 cut(s) 801, 900
TaaI ACNGT 3 cut(s) 16, 928, 1196
TaiI ACGT 3 cut(s) 737, 1188, 1203
TaqI TCGA 4 cut(s) 956, 963, 1023, 1398
TaqII GACCGA 1 cut(s) 1041
TasI AATT 4 cut(s) 606, 627, 670, 1205
TatI WGTACW 1 cut(s) 1115
TauI GCSGC 1 cut(s) 416
TfiI GAWTC 3 cut(s) 426, 724, 1216
Tru1I TTAA 6 cut(s) 189, 243, 674, 695, 933, 1203
Tru9I TTAA 6 cut(s) 189, 243, 674, 695, 933, 1203
TscAI CASTG 4 cut(s) 486, 553, 1059, 1270
TseFI GTSAC 3 cut(s) 79, 179, 730
TseI GCWGC 7 cut(s) 515, 551, 641, 887, 1128, 1296, 1331
Tsp45I GTSAC 3 cut(s) 79, 179, 730
TspDTI ATGAA 4 cut(s) 237, 418, 733, 822
TspGWI ACGGA 2 cut(s) 198, 1167
TspRI CASTG 4 cut(s) 486, 553, 1059, 1270
VpaK11BI GGWCC 1 cut(s) 865
XapI RAATTY 2 cut(s) 670, 1205
XbaI TCTAGA 1 cut(s) 362
XceI RCATGY 1 cut(s) 795
XcmI CCANNNNNNNNNTGG 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 963
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 3 cut(s) 104, 150, 363
Zsp2I ATGCAT 2 cut(s) 793, 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.