MD02G1169800.v1.1

2-alkenal reductase (NADP( )-dependent)-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0028595
Physical Location & Seq
Forward (+)
14682621 .. 14684331
1711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1169800.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGAATGAGGAGGAAGAGGAGAAGAAGAGGAAGATGGTGAAAAATCTCCTTCCCAACAAACAGATTGTGTTGAATCAGTCTGTGAGTGGGTACCCCAAAGAGAGTGACTTTGGGTTGAGATCATCTTTCATCAACTGCAGCCTTTCCGATGATTCCTCACAGGCTGTGCTTCTGAAGAACCTATACCTCTCTTGTGATCCTTACATGCGCCACCGCATGTCCGATCAGGTCGCTCATCCCGGGACTATTATCCAGTCCTTCACTCCTGGTTCTGTCCTTGTGGGATATGGTGTGTCCAAGGTCATGGAGTCCACACATCCAAGGTTCAAGGAAGGTGACTTTGTTTGGGGGATGATTGGGTGGGAGGAGTACAGTTTGATCACTAGCCCGGATAGGCTAAACAAGATCAGTTTTACTGATGTGCCCCTCTCATACTATGCTGGCATTTTGGGAATGCCGGGAATTGCAGCTTACGTTGGATTTCACAAAATATGTTATCCGAAGGAACGCGATTGTGTCTATGTTTCTTCTGCAGCAGGAGGAGTTGGTCAACTTGTTGGACAATTTGCAAAAATGATCGGCTGCTATGTGGTTGGAAGTGCAAGCACTAAAGAGAAAGTTGATCTCTTAAAGCAGAAGATGGGATTTGATGAAGCGTTCAACTATAAGGAGGAAAATTCAGGTTCAGCATTAAGGAAGTACTTCCCCGAAGGCATTGACATTTACTTCGACAATGTCGGTGGTCGCATGCTTGATGAAGTTATCTTGCAGATGAAAGCTCATGGCCGCATCGCACTCTGCGGTATGATTTCACAATACAATATTGAGGAACCAGAGGGTGTGCACAATCTGTTTAGCCTCATCATGAAGCGGATTGAGATGAAAGGGTTCGTAGAGACCGATTTCGATCACGTGTATCCAGAGTATGTTGAGTTTTGTATCAAATACTTGCAGCAAGGGAAGTTGGTCTATGTTGAAGATGTTGCTCAAGGGATAGAGAATGCCCCAGCTGCACTGGTCGGAATTTTCTATGGTCGGAATGTTGGTAAGCAGGTCGTTTGTATTGCGAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N_2 ADH_zinc_N

Protein Analysis

359

Amino Acids

40.1

Weight (kDa)

5.63

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N_2 PF16884 20 - 133 1.3e-24 N-terminal domain of oxidoreductase
ADH_zinc_N PF00107 181 - 313 3.3e-24 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028595)

Species Orthologous Gene IDs
malus_domestica MD02G1169800.v1.1
prunus_persica Prupe.7G140000_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1042
Acc65I GGTACC 1 cut(s) 90
AccB1I GGYRCC 1 cut(s) 90
AccII CGCG 1 cut(s) 510
AciI CCGC 4 cut(s) 214, 787, 801, 871
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 2 cut(s) 676, 1023
AcuI CTGAAG 1 cut(s) 194
AcvI CACGTG 1 cut(s) 913
AfaI GTAC 3 cut(s) 92, 371, 701
AflIII ACRYGT 1 cut(s) 912
AgsI TTSAA 4 cut(s) 73, 328, 661, 977
AjnI CCWGG 1 cut(s) 265
AluBI AGCT 3 cut(s) 470, 779, 1010
AluI AGCT 3 cut(s) 470, 779, 1010
Alw21I GWGCWC 1 cut(s) 846
Alw26I GTCTC 1 cut(s) 890
Alw44I GTGCAC 1 cut(s) 842
AlwI GGATC 1 cut(s) 191
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 784
ApaLI GTGCAC 1 cut(s) 842
ApeKI GCWGC 6 cut(s) 138, 467, 533, 582, 952, 1010
ApoI RAATTY 2 cut(s) 676, 1023
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
Asp700I GAANNNNTTC 2 cut(s) 701, 887
Asp718I GGTACC 1 cut(s) 90
AspLEI GCGC 1 cut(s) 210
AsuC2I CCSGG 4 cut(s) 240, 241, 389, 459
AsuHPI GGTGA 2 cut(s) 49, 347
AvaI CYCGRG 1 cut(s) 239
BaeGI GKGCMC 2 cut(s) 426, 846
BanI GGYRCC 1 cut(s) 90
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbrPI CACGTG 1 cut(s) 913
Bbv12I GWGCWC 1 cut(s) 846
BbvI GCAGC 6 cut(s) 150, 479, 545, 569, 964, 997
BccI CCATC 2 cut(s) 28, 634
BciT130I CCWGG 1 cut(s) 267
BciVI GTATCC 1 cut(s) 927
BclI TGATCA 1 cut(s) 378
BcnI CCSGG 4 cut(s) 240, 241, 389, 459
BcoDI GTCTC 1 cut(s) 890
BfaI CTAG 1 cut(s) 384
BfmI CTRYAG 2 cut(s) 136, 531
BfuAI ACCTGC 1 cut(s) 1042
BfuI GTATCC 1 cut(s) 927
BisI GCNGC 7 cut(s) 139, 468, 534, 583, 787, 953, 1011
BlsI GCNGC 7 cut(s) 140, 469, 535, 584, 788, 954, 1012
BmcAI AGTACT 1 cut(s) 701
Bme1390I CCNGG 5 cut(s) 240, 241, 267, 389, 459
BmeT110I CYCGRG 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 92, 831
BmrFI CCNGG 5 cut(s) 240, 241, 267, 389, 459
BmsI GCATC 1 cut(s) 798
BpuEI CTTGAG 1 cut(s) 972
BpuMI CCSGG 4 cut(s) 240, 241, 389, 459
BsaAI YACGTR 1 cut(s) 913
BsaBI GATNNNNATC 1 cut(s) 906
BsaI GGTCTC 1 cut(s) 890
BsaJI CCNNGG 3 cut(s) 239, 297, 320
Bse1I ACTGG 2 cut(s) 253, 1020
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 239, 297, 320
BseGI GGATG 3 cut(s) 235, 316, 357
BseJI GATNNNNATC 1 cut(s) 906
BseNI ACTGG 2 cut(s) 253, 1020
BseRI GAGGAG 4 cut(s) 23, 32, 380, 555
BseSI GKGCMC 2 cut(s) 426, 846
BseXI GCAGC 6 cut(s) 150, 479, 545, 569, 964, 997
BseYI CCCAGC 1 cut(s) 1006
BsgI GTGCAG 1 cut(s) 996
Bsh1236I CGCG 1 cut(s) 510
BshFI GGCC 1 cut(s) 786
BshNI GGYRCC 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 846
BsiHKCI CYCGRG 1 cut(s) 239
BsiSI CCGG 3 cut(s) 240, 389, 458
BslFI GGGAC 1 cut(s) 256
BsmAI GTCTC 1 cut(s) 890
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 459, 1006
BsnI GGCC 1 cut(s) 786
Bso31I GGTCTC 1 cut(s) 890
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 426, 846
Bsp143I GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
BspACI CCGC 4 cut(s) 214, 787, 801, 871
BspANI GGCC 1 cut(s) 786
BspFNI CGCG 1 cut(s) 510
BspHI TCATGA 1 cut(s) 864
BspLI GGNNCC 2 cut(s) 92, 831
BspMAI CTGCAG 2 cut(s) 140, 535
BspMI ACCTGC 1 cut(s) 1042
BspPI GGATC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 90
BspTNI GGTCTC 1 cut(s) 890
BsrI ACTGG 2 cut(s) 253, 1020
BssECI CCNNGG 3 cut(s) 239, 297, 320
BssMI GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
BssT1I CCWWGG 2 cut(s) 297, 320
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 1 cut(s) 374
Bst6I CTCTTC 2 cut(s) 9, 20
BstBAI YACGTR 1 cut(s) 913
BstC8I GCNNGC 3 cut(s) 442, 604, 749
BstF5I GGATG 3 cut(s) 235, 316, 357
BstFNI CGCG 1 cut(s) 510
BstHHI GCGC 1 cut(s) 210
BstKTI GATC 8 cut(s) 122, 199, 226, 381, 408, 579, 625, 910
BstMAI GTCTC 1 cut(s) 890
BstMBI GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
BstMWI GCNNNNNNNGC 1 cut(s) 1010
BstNI CCWGG 1 cut(s) 267
BstNSI RCATGY 3 cut(s) 208, 220, 751
BstSCI CCNGG 5 cut(s) 238, 239, 265, 387, 457
BstSFI CTRYAG 2 cut(s) 136, 531
BstSLI GKGCMC 2 cut(s) 426, 846
BstUI CGCG 1 cut(s) 510
BstV1I GCAGC 6 cut(s) 150, 479, 545, 569, 964, 997
BstXI CCANNNNNNTGG 1 cut(s) 304
BsuI GTATCC 1 cut(s) 927
BsuRI GGCC 1 cut(s) 786
BtgZI GCGATG 1 cut(s) 775
BtsCI GGATG 3 cut(s) 235, 316, 357
BtsIMutI CAGTG 1 cut(s) 1013
BveI ACCTGC 1 cut(s) 1042
Cac8I GCNNGC 3 cut(s) 442, 604, 749
CciI TCATGA 1 cut(s) 864
CfoI GCGC 1 cut(s) 210
Cfr9I CCCGGG 1 cut(s) 239
Csp6I GTAC 3 cut(s) 91, 370, 700
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviAII CATG 6 cut(s) 205, 217, 304, 748, 782, 865
CviQI GTAC 3 cut(s) 91, 370, 700
DpnI GATC 8 cut(s) 121, 198, 225, 380, 407, 578, 624, 909
DpnII GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
EaeI YGGCCR 1 cut(s) 784
Eam1104I CTCTTC 2 cut(s) 9, 20
EarI CTCTTC 2 cut(s) 9, 20
Eco130I CCWWGG 2 cut(s) 297, 320
Eco31I GGTCTC 1 cut(s) 890
Eco57I CTGAAG 1 cut(s) 194
Eco72I CACGTG 1 cut(s) 913
Eco88I CYCGRG 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 297, 320
ErhI CCWWGG 2 cut(s) 297, 320
FaeI CATG 6 cut(s) 208, 220, 307, 751, 785, 868
FaqI GGGAC 1 cut(s) 256
FatI CATG 6 cut(s) 204, 216, 303, 747, 781, 864
FbaI TGATCA 1 cut(s) 378
Fnu4HI GCNGC 7 cut(s) 139, 468, 534, 583, 787, 953, 1011
FokI GGATG 3 cut(s) 222, 303, 364
Fsp4HI GCNGC 7 cut(s) 139, 468, 534, 583, 787, 953, 1011
FspBI CTAG 1 cut(s) 384
GlaI GCGC 1 cut(s) 209
GluI GCNGC 7 cut(s) 139, 468, 534, 583, 787, 953, 1011
GsaI CCCAGC 1 cut(s) 1010
HaeIII GGCC 1 cut(s) 786
HapII CCGG 3 cut(s) 240, 389, 458
HhaI GCGC 1 cut(s) 210
Hin1II CATG 6 cut(s) 208, 220, 307, 751, 785, 868
Hin6I GCGC 1 cut(s) 208
HinP1I GCGC 1 cut(s) 208
HincII GTYRAC 1 cut(s) 551
HindII GTYRAC 1 cut(s) 551
HinfI GANTC 3 cut(s) 73, 152, 308
HpaII CCGG 3 cut(s) 240, 389, 458
HphI GGTGA 2 cut(s) 49, 347
Hpy166II GTNNAC 3 cut(s) 312, 551, 844
Hpy188I TCNGA 6 cut(s) 148, 174, 223, 501, 1022, 1038
Hpy188III TCNNGA 2 cut(s) 865, 920
Hpy8I GTNNAC 3 cut(s) 312, 551, 844
HpyAV CCTTC 5 cut(s) 59, 268, 326, 496, 704
HpyCH4III ACNGT 1 cut(s) 374
HpyCH4IV ACGT 2 cut(s) 474, 912
HpyCH4V TGCA 9 cut(s) 138, 467, 533, 569, 602, 769, 844, 952, 1013
HpyF10VI GCNNNNNNNGC 1 cut(s) 1010
HpySE526I ACGT 2 cut(s) 474, 912
Hsp92II CATG 6 cut(s) 208, 220, 307, 751, 785, 868
HspAI GCGC 1 cut(s) 208
KpnI GGTACC 1 cut(s) 94
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
Lsp1109I GCAGC 6 cut(s) 150, 479, 545, 569, 964, 997
LweI GCATC 1 cut(s) 798
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 474, 912
MaeIII GTNAC 2 cut(s) 104, 335
MalI GATC 8 cut(s) 121, 198, 225, 380, 407, 578, 624, 909
MboI GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
MboII GAAGA 8 cut(s) 26, 34, 37, 43, 187, 519, 649, 989
MhlI GDGCHC 2 cut(s) 426, 846
MluCI AATT 4 cut(s) 462, 563, 676, 1023
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 5 cut(s) 457, 538, 574, 1000, 1016
MroXI GAANNNNTTC 2 cut(s) 701, 887
MseI TTAA 2 cut(s) 629, 692
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 3 cut(s) 240, 389, 458
MspR9I CCNGG 5 cut(s) 240, 241, 267, 389, 459
Mva1269I GAATGC 2 cut(s) 459, 1006
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 510
MwoI GCNNNNNNNGC 1 cut(s) 1010
NciI CCSGG 4 cut(s) 240, 241, 389, 459
NdeII GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
NlaIII CATG 6 cut(s) 208, 220, 307, 751, 785, 868
NlaIV GGNNCC 2 cut(s) 92, 831
NmuCI GTSAC 2 cut(s) 104, 335
NspI RCATGY 3 cut(s) 208, 220, 751
PaeI GCATGC 1 cut(s) 751
PagI TCATGA 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 897
PctI GAATGC 2 cut(s) 459, 1006
PdmI GAANNNNTTC 2 cut(s) 701, 887
PfeI GAWTC 2 cut(s) 73, 152
PflFI GACNNNGTC 1 cut(s) 734
PkrI GCNGC 7 cut(s) 140, 469, 535, 584, 788, 954, 1012
PleI GAGTC 1 cut(s) 316
PmaCI CACGTG 1 cut(s) 913
PmlI CACGTG 1 cut(s) 913
PpsI GAGTC 1 cut(s) 316
Ppu21I YACGTR 1 cut(s) 913
Psp6I CCWGG 1 cut(s) 265
PspCI CACGTG 1 cut(s) 913
PspFI CCCAGC 1 cut(s) 1006
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 2 cut(s) 92, 831
PstI CTGCAG 2 cut(s) 140, 535
PsyI GACNNNGTC 1 cut(s) 734
PvuII CAGCTG 1 cut(s) 1010
RsaI GTAC 3 cut(s) 92, 371, 701
RsaNI GTAC 3 cut(s) 91, 370, 700
SaqAI TTAA 2 cut(s) 629, 692
SatI GCNGC 7 cut(s) 139, 468, 534, 583, 787, 953, 1011
Sau3AI GATC 8 cut(s) 119, 196, 223, 378, 405, 576, 622, 907
ScaI AGTACT 1 cut(s) 701
SchI GAGTC 1 cut(s) 317
ScrFI CCNGG 5 cut(s) 240, 241, 267, 389, 459
SduI GDGCHC 2 cut(s) 426, 846
SfaNI GCATC 1 cut(s) 798
SfcI CTRYAG 2 cut(s) 136, 531
SmaI CCCGGG 1 cut(s) 241
SmlI CTYRAG 1 cut(s) 987
SmoI CTYRAG 1 cut(s) 987
SphI GCATGC 1 cut(s) 751
Sse9I AATT 4 cut(s) 462, 563, 676, 1023
SsiI CCGC 4 cut(s) 214, 787, 801, 871
SspI AATATT 1 cut(s) 823
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 5 cut(s) 238, 239, 265, 387, 457
StyI CCWWGG 2 cut(s) 297, 320
TaaI ACNGT 1 cut(s) 374
TaiI ACGT 2 cut(s) 477, 915
TaqI TCGA 2 cut(s) 729, 906
TaqII GACCGA 1 cut(s) 914
TasI AATT 4 cut(s) 462, 563, 676, 1023
TatI WGTACW 2 cut(s) 369, 699
TauI GCSGC 1 cut(s) 789
TfiI GAWTC 2 cut(s) 73, 152
Tru1I TTAA 2 cut(s) 629, 692
Tru9I TTAA 2 cut(s) 629, 692
TscAI CASTG 1 cut(s) 1020
TseFI GTSAC 2 cut(s) 104, 335
TseI GCWGC 6 cut(s) 138, 467, 533, 582, 952, 1010
Tsp45I GTSAC 2 cut(s) 104, 335
TspDTI ATGAA 7 cut(s) 17, 118, 666, 771, 788, 881, 896
TspMI CCCGGG 1 cut(s) 239
TspRI CASTG 1 cut(s) 1020
Tth111I GACNNNGTC 1 cut(s) 734
VneI GTGCAC 1 cut(s) 842
XapI RAATTY 2 cut(s) 676, 1023
XceI RCATGY 3 cut(s) 208, 220, 751
XmaI CCCGGG 1 cut(s) 239
XmnI GAANNNNTTC 2 cut(s) 701, 887
XspI CTAG 1 cut(s) 384
ZrmI AGTACT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.