MD02G1295900.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0028592
Physical Location & Seq
Forward (+)
35077794 .. 35078764
971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1295900.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGTTTTTCTTCCACCGCGTCCTAATTTTGGGGCTCTTTCTCGCTATGTGTGTTTTTGTAATCATAAACTCCTTGAGGCAAAAAAAGCACCATGGCTTTCCCGTTTGACCAGTGTTTGGCATGTTCCCCTCTCTCTCTGTGGCACTACTCCAACCCAACCCAACCCATATGAGTTGCTCTCCAAAGTTCTTTGCTGCCAAAATGGAACGATTAGATTCAAAGGCCCTTGGTTTAGCAACCTCGTATGCGTCATCACTTGCGACCCTCGTAACTTGGAGTCCCTTCTCAAGGCTAAGTTCTCCAATTTCCTCAAAGGCCCTTATTTCCAAGACACTGTTCGGGATCTTCTCGGTGATGGCATATTCAGCGCAGACCGCGATAAGTGTCAGTGCCAAAGAAAGATGGCGAGCTTTGAGTTCCAGTTGGCCAAGTTCTGGAAACTGACCGTGGATTCATTGTTTGAGCTGGTTCACGGTGGGTTGTTACCTGTTTTAGAGGACTCGATCAAACACTCGGCCCCAATCAACCTCCAAGACGTTCTTATGAGGTTGACTTTTGACAACGTTTGCATGATCGTATTTGGGGTCGATCTCGATCGCTTGCAGCCAGGTTTACGCAAAATACCATTTTCTCAGGCTATTGAGGAGGCGACAGAAGGAACGTTGATGTGCTTTGTTGTCCCCATGAAGCTTCATATGTCCATAAAAGGAGTGGATGAATTCGCAGACGATGTCATCCGGACAAGAAAGAAAGAGCCAGCTTCACTACTTAATAGTAACGATGAGATTTATTTGGATAAGAAGCAAATTAAATCAGATTTGTTAACGGTGTTTATGGGATTGAAAGATGAGAAGGGGAGAGGCGTCTTCAGAAAAGTTCTTGAGGGATGTATGTGA

Protein Analysis

299

Amino Acids

33.83

Weight (kDa)

8.66

Isoelectric Point (pI)

25.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 78 - 286 1.9e-08 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028592)

Species Orthologous Gene IDs
malus_domestica MD02G1295900.v1.1
pyrus_communis pycom02g24870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 117, 435
AccII CGCG 2 cut(s) 19, 378
AccIII TCCGGA 1 cut(s) 738
AciI CCGC 2 cut(s) 17, 376
AclI AACGTT 2 cut(s) 564, 662
AclWI GGATC 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 719
AcuI CTGAAG 1 cut(s) 853
AcyI GRCGYC 1 cut(s) 864
AfiI CCNNNNNNNGG 4 cut(s) 29, 117, 289, 435
AgsI TTSAA 2 cut(s) 220, 844
AjnI CCWGG 1 cut(s) 607
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 4 cut(s) 411, 466, 691, 761
AluI AGCT 4 cut(s) 411, 466, 691, 761
AlwI GGATC 1 cut(s) 351
Aor13HI TCCGGA 1 cut(s) 738
AoxI GGCC 4 cut(s) 223, 316, 426, 516
ApeKI GCWGC 2 cut(s) 195, 604
ApoI RAATTY 1 cut(s) 719
AspLEI GCGC 1 cut(s) 371
AspS9I GGNCC 3 cut(s) 224, 317, 517
AsuHPI GGTGA 1 cut(s) 365
BalI TGGCCA 1 cut(s) 428
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 859
BbvI GCAGC 2 cut(s) 182, 616
BccI CCATC 2 cut(s) 350, 397
BciT130I CCWGG 1 cut(s) 609
BisI GCNGC 2 cut(s) 196, 605
BlsI GCNGC 2 cut(s) 197, 606
Bme1390I CCNGG 1 cut(s) 609
BmgT120I GGNCC 3 cut(s) 224, 317, 517
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 1 cut(s) 609
BpiI GAAGAC 1 cut(s) 859
BplI GAGNNNNNCTC 2 cut(s) 164, 196
BpuEI CTTGAG 2 cut(s) 95, 272
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 864
BsaJI CCNNGG 3 cut(s) 92, 227, 447
BsaWI WCCGGW 1 cut(s) 738
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 117, 289, 435
Bse1I ACTGG 2 cut(s) 111, 421
Bse8I GATNNNNATC 1 cut(s) 594
BseAI TCCGGA 1 cut(s) 738
BseBI CCWGG 1 cut(s) 609
BseDI CCNNGG 3 cut(s) 92, 227, 447
BseGI GGATG 3 cut(s) 721, 735, 893
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 4 cut(s) 29, 117, 289, 435
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 2 cut(s) 111, 421
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 2 cut(s) 182, 616
Bsh1236I CGCG 2 cut(s) 19, 378
Bsh1285I CGRYCG 1 cut(s) 598
BshFI GGCC 4 cut(s) 225, 318, 428, 518
BsiEI CGRYCG 1 cut(s) 598
BsiSI CCGG 1 cut(s) 739
BslFI GGGAC 2 cut(s) 265, 665
BslI CCNNNNNNNGG 4 cut(s) 29, 117, 289, 435
BsmFI GGGAC 2 cut(s) 265, 665
BsnI GGCC 4 cut(s) 225, 318, 428, 518
Bsp1286I GDGCHC 1 cut(s) 37
Bsp13I TCCGGA 1 cut(s) 738
Bsp143I GATC 5 cut(s) 343, 504, 573, 589, 595
Bsp19I CCATGG 1 cut(s) 92
BspACI CCGC 2 cut(s) 17, 376
BspANI GGCC 4 cut(s) 225, 318, 428, 518
BspCNI CTCAG 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 738
BspFNI CGCG 2 cut(s) 19, 378
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 1 cut(s) 351
BsrI ACTGG 2 cut(s) 111, 421
BssECI CCNNGG 3 cut(s) 92, 227, 447
BssMI GATC 5 cut(s) 343, 504, 573, 589, 595
BssNI GRCGYC 1 cut(s) 864
BssT1I CCWWGG 2 cut(s) 92, 227
Bst2UI CCWGG 1 cut(s) 609
Bst4CI ACNGT 4 cut(s) 337, 448, 476, 829
BstACI GRCGYC 1 cut(s) 864
BstC8I GCNNGC 3 cut(s) 409, 602, 759
BstDEI CTNAG 2 cut(s) 294, 633
BstDSI CCRYGG 2 cut(s) 92, 447
BstENI CCTNNNNNAGG 1 cut(s) 287
BstF5I GGATG 3 cut(s) 721, 735, 893
BstFNI CGCG 2 cut(s) 19, 378
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 5 cut(s) 346, 507, 576, 592, 598
BstMBI GATC 5 cut(s) 343, 504, 573, 589, 595
BstMCI CGRYCG 1 cut(s) 598
BstMWI GCNNNNNNNGC 3 cut(s) 86, 366, 375
BstNI CCWGG 1 cut(s) 609
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 607
BstUI CGCG 2 cut(s) 19, 378
BstV1I GCAGC 2 cut(s) 182, 616
BstV2I GAAGAC 1 cut(s) 859
BstX2I RGATCY 1 cut(s) 343
BstYI RGATCY 1 cut(s) 343
BsuRI GGCC 4 cut(s) 225, 318, 428, 518
BtgI CCRYGG 2 cut(s) 92, 447
BtsCI GGATG 3 cut(s) 721, 735, 893
BtsIMutI CAGTG 3 cut(s) 118, 333, 395
Cac8I GCNNGC 3 cut(s) 409, 602, 759
CfoI GCGC 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 224, 317, 517
CseI GACGC 3 cut(s) 8, 238, 853
CviAII CATG 4 cut(s) 93, 122, 571, 685
DdeI CTNAG 2 cut(s) 294, 633
DpnI GATC 5 cut(s) 345, 506, 575, 591, 597
DpnII GATC 5 cut(s) 343, 504, 573, 589, 595
EaeI YGGCCR 1 cut(s) 426
Eco130I CCWWGG 2 cut(s) 92, 227
Eco24I GRGCYC 1 cut(s) 37
Eco57I CTGAAG 1 cut(s) 853
EcoNI CCTNNNNNAGG 1 cut(s) 287
EcoO109I RGGNCCY 2 cut(s) 224, 317
EcoRI GAATTC 1 cut(s) 719
EcoRII CCWGG 1 cut(s) 607
EcoT14I CCWWGG 2 cut(s) 92, 227
EcoT38I GRGCYC 1 cut(s) 37
ErhI CCWWGG 2 cut(s) 92, 227
FaeI CATG 4 cut(s) 96, 125, 574, 688
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FaqI GGGAC 2 cut(s) 265, 665
FatI CATG 4 cut(s) 92, 121, 570, 684
FauNDI CATATG 2 cut(s) 169, 696
Fnu4HI GCNGC 2 cut(s) 196, 605
FokI GGATG 2 cut(s) 722, 728
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 2 cut(s) 196, 605
GlaI GCGC 1 cut(s) 370
GluI GCNGC 2 cut(s) 196, 605
HaeIII GGCC 4 cut(s) 225, 318, 428, 518
HapII CCGG 1 cut(s) 739
HgaI GACGC 3 cut(s) 8, 238, 853
HhaI GCGC 1 cut(s) 371
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 4 cut(s) 96, 125, 574, 688
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HincII GTYRAC 2 cut(s) 552, 825
HindII GTYRAC 2 cut(s) 552, 825
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 4 cut(s) 216, 278, 452, 500
HpaI GTTAAC 1 cut(s) 825
HpaII CCGG 1 cut(s) 739
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 4 cut(s) 472, 552, 614, 825
Hpy188I TCNGA 2 cut(s) 817, 872
Hpy188III TCNNGA 5 cut(s) 341, 436, 593, 739, 881
Hpy8I GTNNAC 4 cut(s) 472, 552, 614, 825
HpyAV CCTTC 3 cut(s) 293, 650, 847
HpyCH4III ACNGT 4 cut(s) 337, 448, 476, 829
HpyCH4IV ACGT 3 cut(s) 537, 564, 662
HpyCH4V TGCA 2 cut(s) 570, 604
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 366, 375
HpyF3I CTNAG 2 cut(s) 294, 633
HpySE526I ACGT 3 cut(s) 537, 564, 662
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 4 cut(s) 96, 125, 574, 688
HspAI GCGC 1 cut(s) 369
Kpn2I TCCGGA 1 cut(s) 738
KspAI GTTAAC 1 cut(s) 825
Kzo9I GATC 5 cut(s) 343, 504, 573, 589, 595
Lsp1109I GCAGC 2 cut(s) 182, 616
MaeII ACGT 3 cut(s) 537, 564, 662
MaeIII GTNAC 3 cut(s) 269, 483, 776
MalI GATC 5 cut(s) 345, 506, 575, 591, 597
MboI GATC 5 cut(s) 343, 504, 573, 589, 595
MboII GAAGA 2 cut(s) 338, 859
MflI RGATCY 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 37
MlsI TGGCCA 1 cut(s) 428
MluCI AATT 4 cut(s) 25, 304, 719, 807
MluNI TGGCCA 1 cut(s) 428
MlyI GAGTC 2 cut(s) 287, 494
MmeI TCCRAC 1 cut(s) 176
Mox20I TGGCCA 1 cut(s) 428
MroI TCCGGA 1 cut(s) 738
MscI TGGCCA 1 cut(s) 428
MseI TTAA 3 cut(s) 771, 810, 824
Msp20I TGGCCA 1 cut(s) 428
MspI CCGG 1 cut(s) 739
MspR9I CCNGG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 609
MvnI CGCG 2 cut(s) 19, 378
MwoI GCNNNNNNNGC 3 cut(s) 86, 366, 375
NcoI CCATGG 1 cut(s) 92
NdeI CATATG 2 cut(s) 169, 696
NdeII GATC 5 cut(s) 343, 504, 573, 589, 595
NlaIII CATG 4 cut(s) 96, 125, 574, 688
NlaIV GGNNCC 1 cut(s) 519
NmeAIII GCCGAG 1 cut(s) 494
NspI RCATGY 1 cut(s) 125
PfeI GAWTC 2 cut(s) 216, 452
PflFI GACNNNGTC 1 cut(s) 731
PflMI CCANNNNNTGG 2 cut(s) 117, 435
PkrI GCNGC 2 cut(s) 197, 606
Ple19I CGATCG 1 cut(s) 598
PleI GAGTC 2 cut(s) 286, 494
PpsI GAGTC 2 cut(s) 286, 494
Psp1406I AACGTT 2 cut(s) 564, 662
Psp6I CCWGG 1 cut(s) 607
PspGI CCWGG 1 cut(s) 607
PspN4I GGNNCC 1 cut(s) 519
PspPI GGNCC 3 cut(s) 224, 317, 517
PsuI RGATCY 1 cut(s) 343
PsyI GACNNNGTC 1 cut(s) 731
PvuI CGATCG 1 cut(s) 598
SaqAI TTAA 3 cut(s) 771, 810, 824
SatI GCNGC 2 cut(s) 196, 605
Sau3AI GATC 5 cut(s) 343, 504, 573, 589, 595
Sau96I GGNCC 3 cut(s) 224, 317, 517
SchI GAGTC 2 cut(s) 287, 494
ScrFI CCNGG 1 cut(s) 609
SduI GDGCHC 1 cut(s) 37
SmlI CTYRAG 3 cut(s) 74, 287, 881
SmoI CTYRAG 3 cut(s) 74, 287, 881
Sse9I AATT 4 cut(s) 25, 304, 719, 807
SsiI CCGC 2 cut(s) 17, 376
StyD4I CCNGG 1 cut(s) 607
StyI CCWWGG 2 cut(s) 92, 227
TaaI ACNGT 4 cut(s) 337, 448, 476, 829
TaiI ACGT 3 cut(s) 540, 567, 665
TaqI TCGA 3 cut(s) 503, 588, 594
TasI AATT 4 cut(s) 25, 304, 719, 807
TfiI GAWTC 2 cut(s) 216, 452
Tru1I TTAA 3 cut(s) 771, 810, 824
Tru9I TTAA 3 cut(s) 771, 810, 824
TscAI CASTG 3 cut(s) 118, 340, 395
TseI GCWGC 2 cut(s) 195, 604
TspDTI ATGAA 4 cut(s) 444, 683, 701, 732
TspRI CASTG 3 cut(s) 118, 340, 395
Tth111I GACNNNGTC 1 cut(s) 731
Van91I CCANNNNNTGG 2 cut(s) 117, 435
XagI CCTNNNNNAGG 1 cut(s) 287
XapI RAATTY 1 cut(s) 719
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.