MD02G1320700.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0018709
Physical Location & Seq
Reverse (-)
37531291 .. 37543143
11853 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1320700.v1.1.491

Sequence Viewer

Length: 4629 bp
ATGGGAACTCCGATAAACATCATAGTTGGATCACATGTTTGGGCAGAGGATTCAGAGGCTGCGTGGATTGATGCCCAAGTCAAAGAAATTCATGGCAGAGATGCCACCATTATTACCACAAACGGGAAGACTATGGTTACAGACATGTCCAGTATGTACCCAAAAGACACAGAAGCACCACCAGCAGGCGTTGACGACATGACTAAGTTGGCTTACCTCCATGAGCCGGGAGTCCTGCATAACTTAGCCTGTCGGTTTTCTATCAATGAGATATATACTTACACTGGGAACATCCTAATAGCGGTGAATCCATTCCGGAGACTACCACACTTGTATGACACCCACATGATGGAGCAGTACAAAGGAACTGCTTTTGGGGAGCTAAGTCCGCACCTTTTTGCTGTTGCAGACACTTGTTACAGGGCAATGATCAATGAACAAGGAAGCCAGTCAATTCTGGTCAGTGGGGAGAGTGGTGCTGGTAAAACAGAGACAACAAAGATGCTCATGAGATATCTTGCCTTCATGGGAGGCAGATCAGGAACAGAAGGGAGAACAGTGGAACAACAGGTCTTAGAGTCCAACCCAGTCTTGGAGGCATTCGGGAACGCCAAAACTGTGAAAAACAACAATTCGAGTCGCTTCGGTAAATTTGTAGAGATCCAATTTGATAAGCATTGGAAAATCTCTGGAGCTGCTATTCGTACATATCTTCTTGAAAGATCACGTGTTTGCCAAGTCTCTGACCCAGAGAGAAACTACCATTGCTTTTACATGCTTTGTGCGGCACCAGCAGAGGATGTGAAGAGGTACAAGCTAGGAGACCCAAGAACTTTCCATTATCTTAATCAATCCTCCTGCTATGAAGTTGCAAATGTAGATGACGCAAGAGAGTATTTGGAGACCAGAAATGCTATGGATGTTGTTGGGATCAGCCAGGAAGAGCAGGATGCCATATTCCGAGTGGTTGCTGCAATCCTCCATCTTGGGAACATTGACTTCATCAAAGGGGACGAAGTTGATTCTTCCAAGTTAAAAGATGATAAATCATGCTCTCACCTCCAAACTTCTGCAGAGCTACTCATGTGTGATAACAAGTCTTTGGAAGACTCGCTTTGCAAGCGTGTAATAGTGACTCCTGATGGTAACATTACAAAACCTCTTGATCCAGACTCAGCTACATTAAGTCGTGATGCCCTGGCGAAGACTGTCTACTCCAGACTGTTCGATTGGATTGTGGATAAGATAAACAACTCAATTGGTCAGGATCCAAACTCATCAAGCTTAATAGGAGTCCTTGATATTTATGGCTTCGAAAGCTTCAAGATCAACAGCTTTGAGCAGCTATGTATCAACCTAACAAATGAAAAGCTGCAACAACATTTTAATCAGCATGTGTTCAAGATGGAGCAAGAAGAGTACACAAAGGATGAAATAAACTGGAGCTATGTAGAATTTGTAGATAACCAGGATGTTTTGGATCTTATCGAGAGGAAGCCTGGGGGCATAATTGCCCTTCTCGATGAAGCATGTATGTTTCCCAAGTCAACGCATGAGACATTTGCACAGAAGATGTATCAGACATATAAAGGACACAAGCGATTTAAAAAGCCAAAACTTGCTCGAACAGACTTCACAGTTAACCACTATGCTGGAGATGTGACATACCAAACAGACCATTTTCTTGATAAAAACAAGGATTACGTAGTGGCAGAACATCAAGCTCTTTTGAATGCCTCGAACTGCTCATTTGTCTCAAATCTATTCCCTCCACTGCCTGAGGAGACTTCAAAACAATCCAAGTTTTCTTCCATAGGCACCCGTTTCAAGCAACAACTGCAAGCTTTAATGGAGACACTTAGCACAACAGAACCACATTATATCAGATGTGTTAAGCCAAATACAGTTTTGAAGCCTGGAATTTTTGAGAATGCCAACGTATTAAACCAGTTGAGGTGTGGGGGTGTGCTGGAGGCTATTAGGATTAGTTGTGCTGGATATCCAACAAAAAGAACATTTGATGATTTCCTCGACCGCTTTGGAATGTTGGCTCCTGATATTATGGACGGATCTGATGAAAAATCAGCATGCATTGCCATCTGTAACAGGATGGGACTGAAGGGATACCAGATTGGAAAATCAAAGGTTTTTCTTAGAGCTGGTCAGATGGCTGAATTAGATGCACGCAGAACTGAAGTTTTGGTTAATTCTGCCAGATGCATTCAAAGGCAAATCAGAACACATCTAACCAGAAGGGAGTTCACTGCCCTGCGAAGGGCTGCTATTCACTTGCAGAAACATTGGAGAGGACAACTTGCGCGCAAGTTGTATGAACAAATAAGACAGGAAGCTGCTTCAATCCGTATACAAAAACGTCTACGTGCTCACAAAGCCAGAATATCCTACACAAAATTGCAGGCAGCAGCAGTTGCCATCCAAACCGGGTTACGAACTATGGTGGCCCGAAATGATCATCAAAACAGGAGAAGAACCAAGGCTGCAAAAATTATTCAGACTATTTGGCGAAGATTTGAAGCTCTTTCTGAATATAAACACCAGAAAAAGGCGAGTCTAACTCTTCAATGTCTCTGGAGAGCAAGGGTAGCAAGGAAGGAGCTTAGGAAGCTTAGGATGGCTGCGAGAGAAACAGGAGCGCTTAAGGCAGCTAAAGACATGCTGGAGAAGCGTGTCGAAGAGCTAACATGGCGACTGGAATTTGAAAAGCACTTGAGGATTGATCTTGACGAAACTAAAGGAAGAGAAATTGCAAAGTTGCAACATTGTTTGCATGAAATGCAAAGGCAAATAGATGAAGCCCATGCAGCAATCATCCATGAGAAAGAAGCAGCAAAGTTAGCCATTAAACAAGCTCCCCCGGTCATCAAAGAGGTGGCAGTTGTGGATAACACCAAACTAGAAATACTGGAAAGCCGCAATGAGGAACTACAGGGTGAGCTAAGTGACCTGAAGAAGAAACTTGAAGAATTTGAAGAAAATCACTCTCAAATTGAAGAAGAAAGGAAAGCAAGGCATGAGGAGGCAGAAGAAGCACGGCTGAAATCAGTGCACATTCAGGAGACTATTGAAAGGTTACAGTTGAACTTGTCAAGTCTTGAGTCTGAAAATCAGGTGCTACGCCAACAGGCTCTTGTTCCATCAGCAAATGGGGAGCTCTCAGAAGAACTGAGAATCTTGAAGAGTAAGATGGCAGAATTGGAATCAGATAATAAATTACTTCACAGCAAGGCTGTAGTCGTGGAGCAAAAAGCTAATCCAGAGAGGGTTCAGCCACTAGTAAAGGAGCCAGATCCTGTTGTGTCCCACCTTACTAGACAAAGATCCCTCACGGATAGGCAGCAGGAAAATCTTGATGTGCTTGTTAAGTGTCTTGTGGAAGACAGACAATTTGACAAGAAGAGGCCTGTCGCTGCCTGTATTATTTACAAAGCACTTGTTCAGTGGAGAACCTTCGAAGCAGAGAAAACAAACATATTTGATAGGATTATTCACAAAATTCGGTCTATGGTAGAGAGTCAGAACAATATCGATGATCTGGCATATTGGCTTTCAACAACTTGTACCCTCGTATTTCTTGTACAAAGTACACTCAAGGCAACCAGTGCACCTGATTCAGCTTCACGTCGTAACCAAACTTCTCCTGCTACCATATTTGGTAGAAATAGAACGGCACAAGGTTTTCATTCATCTTCCATTACTATGGGAGTTTCAAGTGGCTACAGTGGAATGGCTGGAAAGCCCAACGGGAAATTAAAAGTGCAGGCCAAGTACCCAGCATTGTTATTCAAGCAACATCTAACTGCATTTGTCGAGAAAATTTATGGGACCATCCGTGACATTCTAAAGAAGGAGATTAGCCCATTCTTGAATTTGTGCATTCAGGCACCGAGATCTACAAGGGCCAAGTCAATGAGAGGCTCGTCCAAAACTATACATTCAAATATAAGAGCAAAACAATCACCATTGAACACGCACTGGCAAAGCACTGTCAACAAGTTAGATCATACCTTAGATATTTTGTCTATGAATCATGTCTCTCCTATGATCGCAAGGAAAATGTTTACTCAAGTTTTCTCATTCATAAATGTCCAGCTCTTTAACAGCTTATTGCTTCGTCGTGAGTGCTGCTCATTTCGCAATGGAGAATTTCTGAAAGCAGGTTTGCTTAAATTGGAACAGTGGTGTCTCAAAGTAACAGATCAGTTTGCTGGATCGTCCTGGGATGAGCTTCAACACATAAGGCAGGCTGTCAGATTTTTGGTTTTGCACCAAAAAACTCAGAAATCGGTGGATGAAATCACAAATGAATTGTGTCCGATACTCAGCATCCCTCAAATATATCGCATTGGAACAATGTTCTGGGACGACAAATATGGAGCCCAGGGATTATCTTCAGACGTTATTGGACAAATGAGAGTTATGATGGCGGAGGATTCAATCAACATGAACATGCTCAATAATTCCTTCCTGCTTGACGTTGATTCCAGCATACCTTTTTCGATGGAAGAAATGTGCACATCCTTCATTGATATCAGCCTATCTGATGTGGATCCACCGGCCATCATCCACCAAAGATCTGACTTCCACGTCCTGTTGCAACAAACAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016461 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIL IQ Myosin_N Myosin_head

Protein Analysis

1543

Amino Acids

175.76

Weight (kDa)

8.53

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_N PF02736 9 - 47 9.3e-08 Myosin N-terminal SH3-like domain
Myosin_head PF00063 64 - 719 2.2e-252 Myosin head (motor domain)
DIL PF01843 1358 - 1462 2.8e-28 DIL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018709)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31900 AT2G31900 AT2G31900 AT2G31900
malus_domestica MD02G1320700.v1.1
prunus_persica Prupe.2G000500_v2.0.a1 Prupe.2G000500_v2.0.a1 Prupe.2G000500_v2.0.a1
pyrus_communis pycom02g26860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 4604
Acc36I ACCTGC 1 cut(s) 4166
AccB1I GGYRCC 3 cut(s) 787, 1817, 3901
AccB7I CCANNNNNTGG 1 cut(s) 349
AccI GTMKAC 3 cut(s) 1212, 2365, 2377
AccII CGCG 1 cut(s) 2320
AccIII TCCGGA 1 cut(s) 315
AciI CCGC 6 cut(s) 302, 389, 785, 2035, 2932, 4445
AcoI YGGCCR 1 cut(s) 4575
AcuI CTGAAG 4 cut(s) 2138, 2214, 2987, 4395
AcvI CACGTG 1 cut(s) 728
AfaI GTAC 9 cut(s) 158, 359, 706, 812, 1421, 3580, 3597, 3604, 3788
AfeI AGCGCT 1 cut(s) 2655
AflII CTTAAG 1 cut(s) 2657
AflIII ACRYGT 3 cut(s) 34, 144, 727
AhlI ACTAGT 1 cut(s) 3292
AjiI CACGTC 2 cut(s) 3641, 4606
AjnI CCWGG 7 cut(s) 936, 1197, 1467, 1498, 1915, 4235, 4398
Alw21I GWGCWC 5 cut(s) 2386, 3069, 3174, 3625, 4535
Alw44I GTGCAC 3 cut(s) 3065, 3621, 4531
AlwNI CAGNNNCTG 2 cut(s) 59, 3311
Aor13HI TCCGGA 1 cut(s) 315
Aor51HI AGCGCT 1 cut(s) 2655
AoxI GGCC 5 cut(s) 2460, 3419, 3780, 3918, 4575
ApaLI GTGCAC 3 cut(s) 3065, 3621, 4531
ArsI GACNNNNNNTTYG 2 cut(s) 3389, 3421
Asp700I GAANNNNTTC 1 cut(s) 311
AspLEI GCGC 3 cut(s) 2320, 2322, 2656
AspS9I GGNCC 3 cut(s) 2461, 3843, 3918
AsuC2I CCSGG 3 cut(s) 228, 2443, 2876
AsuHPI GGTGA 4 cut(s) 316, 1049, 2963, 3969
AsuII TTCGAA 2 cut(s) 1314, 3471
AvaII GGWCC 1 cut(s) 3843
AxyI CCTNAGG 1 cut(s) 1779
BaeGI GKGCMC 3 cut(s) 3069, 3625, 4535
BamHI GGATCC 2 cut(s) 1267, 4567
BanI GGYRCC 3 cut(s) 787, 1817, 3901
BanII GRGCYC 2 cut(s) 3174, 4399
BarI GAAGNNNNNNTAC 4 cut(s) 1196, 1228, 3637, 3669
BbrPI CACGTG 1 cut(s) 728
BbsI GAAGAC 4 cut(s) 134, 1113, 1211, 3402
Bbv12I GWGCWC 5 cut(s) 2386, 3069, 3174, 3625, 4535
BceAI ACGGC 2 cut(s) 3068, 3702
BciT130I CCWGG 7 cut(s) 938, 1199, 1469, 1500, 1917, 4237, 4400
BciVI GTATCC 1 cut(s) 2117
BclI TGATCA 2 cut(s) 429, 2470
BcnI CCSGG 3 cut(s) 228, 2443, 2876
BcuI ACTAGT 1 cut(s) 3292
BfaI CTAG 4 cut(s) 818, 2915, 3293, 3330
BfmI CTRYAG 4 cut(s) 1071, 2945, 3249, 3736
BfoI RGCGCY 1 cut(s) 2657
BfrI CTTAAG 1 cut(s) 2657
BfuAI ACCTGC 1 cut(s) 4166
BfuI GTATCC 1 cut(s) 2117
BglII AGATCT 2 cut(s) 3908, 4592
Bme18I GGWCC 1 cut(s) 3843
BmgBI CACGTC 2 cut(s) 3641, 4606
BmgT120I GGNCC 3 cut(s) 2461, 3843, 3918
BmiI GGNNCC 9 cut(s) 789, 1269, 1819, 2052, 3303, 3844, 3903, 4396, 4569
BmrI ACTGGG 2 cut(s) 294, 581
BmsI GCATC 8 cut(s) 61, 91, 492, 940, 1183, 2170, 2207, 4353
BmuI ACTGGG 2 cut(s) 294, 581
BpiI GAAGAC 4 cut(s) 134, 1113, 1211, 3402
BplI GAGNNNNNCTC 4 cut(s) 2560, 2592, 4130, 4162
BpmI CTGGAG 7 cut(s) 711, 1201, 1462, 1674, 1991, 2611, 2699
Bpu10I CCTNAGC 2 cut(s) 2618, 2627
Bpu14I TTCGAA 2 cut(s) 1314, 3471
BpuEI CTTGAG 4 cut(s) 2749, 3134, 3593, 4068
BpuMI CCSGG 3 cut(s) 228, 2443, 2876
Bsa29I ATCGAT 1 cut(s) 3546
BsaAI YACGTR 3 cut(s) 728, 1705, 2381
BsaBI GATNNNNATC 2 cut(s) 17, 4566
BsaI GGTCTC 2 cut(s) 816, 896
BsaJI CCNNGG 7 cut(s) 1197, 1499, 2493, 2874, 4236, 4398, 4399
BsaWI WCCGGW 1 cut(s) 315
Bse118I RCCGGY 1 cut(s) 4573
Bse21I CCTNAGG 1 cut(s) 1779
Bse3DI GCAATG 5 cut(s) 432, 763, 2091, 2941, 4162
Bse8I GATNNNNATC 2 cut(s) 17, 4566
BseAI TCCGGA 1 cut(s) 315
BseBI CCWGG 7 cut(s) 938, 1199, 1469, 1500, 1917, 4237, 4400
BseCI ATCGAT 1 cut(s) 3546
BseDI CCNNGG 7 cut(s) 1197, 1499, 2493, 2874, 4236, 4398, 4399
BseJI GATNNNNATC 2 cut(s) 17, 4566
BseMI GCAATG 5 cut(s) 432, 763, 2091, 2941, 4162
BseMII CTCAG 6 cut(s) 1188, 1770, 3176, 3189, 4310, 4354
BsePI GCGCGC 1 cut(s) 2318
BseRI GAGGAG 2 cut(s) 1796, 3050
BseSI GKGCMC 3 cut(s) 3069, 3625, 4535
BseYI CCCAGC 1 cut(s) 3790
BsgI GTGCAG 1 cut(s) 3797
Bsh1236I CGCG 1 cut(s) 2320
Bsh1285I CGRYCG 1 cut(s) 2035
BshFI GGCC 5 cut(s) 2462, 3421, 3782, 3920, 4577
BshNI GGYRCC 3 cut(s) 787, 1817, 3901
BshVI ATCGAT 1 cut(s) 3546
BsiEI CGRYCG 1 cut(s) 2035
BsiHKAI GWGCWC 5 cut(s) 2386, 3069, 3174, 3625, 4535
BsiSI CCGG 5 cut(s) 227, 316, 2442, 2876, 4574
BslFI GGGAC 5 cut(s) 1025, 2127, 3304, 3856, 4394
BsmFI GGGAC 5 cut(s) 1025, 2127, 3304, 3856, 4394
BsmI GAATGC 5 cut(s) 599, 1738, 1936, 2220, 3894
BsnI GGCC 5 cut(s) 2462, 3421, 3782, 3920, 4577
Bso31I GGTCTC 2 cut(s) 816, 896
Bsp119I TTCGAA 2 cut(s) 1314, 3471
Bsp1286I GDGCHC 6 cut(s) 2386, 3069, 3174, 3625, 4399, 4535
Bsp13I TCCGGA 1 cut(s) 315
Bsp1407I TGTACA 1 cut(s) 3595
BspACI CCGC 6 cut(s) 302, 389, 785, 2035, 2932, 4445
BspANI GGCC 5 cut(s) 2462, 3421, 3782, 3920, 4577
BspCNI CTCAG 6 cut(s) 1187, 1771, 3177, 3188, 4309, 4353
BspDI ATCGAT 1 cut(s) 3546
BspEI TCCGGA 1 cut(s) 315
BspFNI CGCG 1 cut(s) 2320
BspHI TCATGA 1 cut(s) 507
BspLI GGNNCC 9 cut(s) 789, 1269, 1819, 2052, 3303, 3844, 3903, 4396, 4569
BspMAI CTGCAG 1 cut(s) 1075
BspMI ACCTGC 1 cut(s) 4166
BspQI GCTCTTC 2 cut(s) 936, 2688
BspT104I TTCGAA 2 cut(s) 1314, 3471
BspT107I GGYRCC 3 cut(s) 787, 1817, 3901
BspTI CTTAAG 1 cut(s) 2657
BspTNI GGTCTC 2 cut(s) 816, 896
BsrDI GCAATG 5 cut(s) 432, 763, 2091, 2941, 4162
BsrFI RCCGGY 1 cut(s) 4573
BsrGI TGTACA 1 cut(s) 3595
BssAI RCCGGY 1 cut(s) 4573
BssECI CCNNGG 7 cut(s) 1197, 1499, 2493, 2874, 4236, 4398, 4399
BssHII GCGCGC 1 cut(s) 2318
BssNAI GTATAC 1 cut(s) 2366
BssT1I CCWWGG 1 cut(s) 2493
Bst1107I GTATAC 1 cut(s) 2366
Bst2UI CCWGG 7 cut(s) 938, 1199, 1469, 1500, 1917, 4237, 4400
Bst6I CTCTTC 8 cut(s) 800, 936, 1410, 2583, 2688, 2752, 3191, 3410
BstAFI CTTAAG 1 cut(s) 2657
BstAPI GCANNNNNTGC 5 cut(s) 1837, 2093, 2429, 2794, 3620
BstAUI TGTACA 1 cut(s) 3595
BstBAI YACGTR 3 cut(s) 728, 1705, 2381
BstBI TTCGAA 2 cut(s) 1314, 3471
BstC8I GCNNGC 9 cut(s) 187, 1121, 1842, 2089, 2185, 2320, 2418, 3780, 4263
BstFNI CGCG 1 cut(s) 2320
BstH2I RGCGCY 1 cut(s) 2657
BstHHI GCGC 3 cut(s) 2320, 2322, 2656
BstMCI CGRYCG 1 cut(s) 2035
BstNI CCWGG 7 cut(s) 938, 1199, 1469, 1500, 1917, 4237, 4400
BstNSI RCATGY 8 cut(s) 38, 148, 778, 1397, 1533, 2091, 2677, 4471
BstSFI CTRYAG 4 cut(s) 1071, 2945, 3249, 3736
BstSLI GKGCMC 3 cut(s) 3069, 3625, 4535
BstSNI TACGTA 1 cut(s) 1705
BstUI CGCG 1 cut(s) 2320
BstV2I GAAGAC 4 cut(s) 134, 1113, 1211, 3402
BstX2I RGATCY 9 cut(s) 660, 1267, 1480, 2069, 3307, 3338, 3908, 4567, 4592
BstXI CCANNNNNNTGG 2 cut(s) 1652, 3718
BstYI RGATCY 9 cut(s) 660, 1267, 1480, 2069, 3307, 3338, 3908, 4567, 4592
BstZ17I GTATAC 1 cut(s) 2366
Bsu15I ATCGAT 1 cut(s) 3546
Bsu36I CCTNAGG 1 cut(s) 1779
BsuI GTATCC 1 cut(s) 2117
BsuRI GGCC 5 cut(s) 2462, 3421, 3782, 3920, 4577
BsuTUI ATCGAT 1 cut(s) 3546
BtrI CACGTC 2 cut(s) 3641, 4606
BtsI GCAGTG 2 cut(s) 1772, 2262
BveI ACCTGC 1 cut(s) 4166
Cac8I GCNNGC 9 cut(s) 187, 1121, 1842, 2089, 2185, 2320, 2418, 3780, 4263
CaiI CAGNNNCTG 2 cut(s) 59, 3311
CciI TCATGA 1 cut(s) 507
CfoI GCGC 3 cut(s) 2320, 2322, 2656
Cfr10I RCCGGY 1 cut(s) 4573
Cfr13I GGNCC 3 cut(s) 2461, 3843, 3918
ClaI ATCGAT 1 cut(s) 3546
CseI GACGC 1 cut(s) 893
Csp6I GTAC 9 cut(s) 157, 358, 705, 811, 1420, 3579, 3596, 3603, 3787
CviQI GTAC 9 cut(s) 157, 358, 705, 811, 1420, 3579, 3596, 3603, 3787
DraI TTTAAA 1 cut(s) 1606
DrdI GACNNNNNNGTC 1 cut(s) 4604
DseDI GACNNNNNNGTC 1 cut(s) 4604
EaeI YGGCCR 1 cut(s) 4575
Eam1104I CTCTTC 8 cut(s) 800, 936, 1410, 2583, 2688, 2752, 3191, 3410
EarI CTCTTC 8 cut(s) 800, 936, 1410, 2583, 2688, 2752, 3191, 3410
EciI GGCGGA 1 cut(s) 4460
Ecl136II GAGCTC 1 cut(s) 3172
Eco105I TACGTA 1 cut(s) 1705
Eco130I CCWWGG 1 cut(s) 2493
Eco147I AGGCCT 1 cut(s) 3421
Eco24I GRGCYC 2 cut(s) 3174, 4399
Eco31I GGTCTC 2 cut(s) 816, 896
Eco32I GATATC 3 cut(s) 515, 2000, 4549
Eco47I GGWCC 1 cut(s) 3843
Eco47III AGCGCT 1 cut(s) 2655
Eco53kI GAGCTC 1 cut(s) 3172
Eco57I CTGAAG 4 cut(s) 2138, 2214, 2987, 4395
Eco72I CACGTG 1 cut(s) 728
Eco81I CCTNAGG 1 cut(s) 1779
EcoICRI GAGCTC 1 cut(s) 3172
EcoRII CCWGG 7 cut(s) 936, 1197, 1467, 1498, 1915, 4235, 4398
EcoRV GATATC 3 cut(s) 515, 2000, 4549
EcoT14I CCWWGG 1 cut(s) 2493
EcoT22I ATGCAT 2 cut(s) 2093, 2222
EcoT38I GRGCYC 2 cut(s) 3174, 4399
ErhI CCWWGG 1 cut(s) 2493
FalI AAGNNNNNCTT 2 cut(s) 1098, 1130
FaqI GGGAC 5 cut(s) 1025, 2127, 3304, 3856, 4394
FbaI TGATCA 2 cut(s) 429, 2470
FblI GTMKAC 3 cut(s) 1212, 2365, 2377
FriOI GRGCYC 2 cut(s) 3174, 4399
FspBI CTAG 4 cut(s) 818, 2915, 3293, 3330
GlaI GCGC 3 cut(s) 2319, 2321, 2655
GsaI CCCAGC 1 cut(s) 3794
GsuI CTGGAG 7 cut(s) 711, 1201, 1462, 1674, 1991, 2611, 2699
HaeII RGCGCY 1 cut(s) 2657
HaeIII GGCC 5 cut(s) 2462, 3421, 3782, 3920, 4577
HapII CCGG 5 cut(s) 227, 316, 2442, 2876, 4574
HgaI GACGC 1 cut(s) 893
HhaI GCGC 3 cut(s) 2320, 2322, 2656
Hin6I GCGC 3 cut(s) 2318, 2320, 2654
HinP1I GCGC 3 cut(s) 2318, 2320, 2654
HincII GTYRAC 4 cut(s) 193, 1548, 1642, 4009
HindII GTYRAC 4 cut(s) 193, 1548, 1642, 4009
HindIII AAGCTT 4 cut(s) 1282, 1318, 1842, 2624
HpaI GTTAAC 1 cut(s) 1642
HpaII CCGG 5 cut(s) 227, 316, 2442, 2876, 4574
HphI GGTGA 4 cut(s) 316, 1049, 2963, 3969
Hpy99I CGWCG 2 cut(s) 3645, 4137
HpyCH4IV ACGT 9 cut(s) 727, 1704, 1938, 2374, 2380, 3640, 4416, 4494, 4605
HpySE526I ACGT 9 cut(s) 727, 1704, 1938, 2374, 2380, 3640, 4416, 4494, 4605
HspAI GCGC 3 cut(s) 2318, 2320, 2654
Kpn2I TCCGGA 1 cut(s) 315
Ksp22I TGATCA 2 cut(s) 429, 2470
KspAI GTTAAC 1 cut(s) 1642
LguI GCTCTTC 2 cut(s) 936, 2688
LweI GCATC 8 cut(s) 61, 91, 492, 940, 1183, 2170, 2207, 4353
MaeI CTAG 4 cut(s) 818, 2915, 3293, 3330
MaeII ACGT 9 cut(s) 727, 1704, 1938, 2374, 2380, 3640, 4416, 4494, 4605
MfeI CAATTG 1 cut(s) 1257
MflI RGATCY 9 cut(s) 660, 1267, 1480, 2069, 3307, 3338, 3908, 4567, 4592
MhlI GDGCHC 6 cut(s) 2386, 3069, 3174, 3625, 4399, 4535
MmeI TCCRAC 3 cut(s) 7, 606, 2027
Mph1103I ATGCAT 2 cut(s) 2093, 2222
MroI TCCGGA 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 311
MslI CAYNNNNRTG 4 cut(s) 333, 344, 3716, 4466
MspCI CTTAAG 1 cut(s) 2657
MspI CCGG 5 cut(s) 227, 316, 2442, 2876, 4574
MunI CAATTG 1 cut(s) 1257
Mva1269I GAATGC 5 cut(s) 599, 1738, 1936, 2220, 3894
MvaI CCWGG 7 cut(s) 938, 1199, 1469, 1500, 1917, 4237, 4400
MvnI CGCG 1 cut(s) 2320
NciI CCSGG 3 cut(s) 228, 2443, 2876
NlaIV GGNNCC 9 cut(s) 789, 1269, 1819, 2052, 3303, 3844, 3903, 4396, 4569
NmuCI GTSAC 4 cut(s) 1132, 1660, 2960, 3851
NsiI ATGCAT 2 cut(s) 2093, 2222
NspI RCATGY 8 cut(s) 38, 148, 778, 1397, 1533, 2091, 2677, 4471
NspV TTCGAA 2 cut(s) 1314, 3471
PaeI GCATGC 1 cut(s) 2091
PagI TCATGA 1 cut(s) 507
PasI CCCWGGG 1 cut(s) 4399
PauI GCGCGC 1 cut(s) 2318
PceI AGGCCT 1 cut(s) 3421
PciI ACATGT 2 cut(s) 34, 144
PciSI GCTCTTC 2 cut(s) 936, 2688
PctI GAATGC 5 cut(s) 599, 1738, 1936, 2220, 3894
PdmI GAANNNNTTC 1 cut(s) 311
PfeI GAWTC 9 cut(s) 50, 307, 1022, 3189, 3218, 3629, 4045, 4451, 4499
PflMI CCANNNNNTGG 1 cut(s) 349
PmaCI CACGTG 1 cut(s) 728
PmlI CACGTG 1 cut(s) 728
Ppu21I YACGTR 3 cut(s) 728, 1705, 2381
PscI ACATGT 2 cut(s) 34, 144
Psp124BI GAGCTC 1 cut(s) 3174
Psp6I CCWGG 7 cut(s) 936, 1197, 1467, 1498, 1915, 4235, 4398
PspCI CACGTG 1 cut(s) 728
PspFI CCCAGC 1 cut(s) 3790
PspGI CCWGG 7 cut(s) 936, 1197, 1467, 1498, 1915, 4235, 4398
PspN4I GGNNCC 9 cut(s) 789, 1269, 1819, 2052, 3303, 3844, 3903, 4396, 4569
PspPI GGNCC 3 cut(s) 2461, 3843, 3918
PstI CTGCAG 1 cut(s) 1075
PstNI CAGNNNCTG 2 cut(s) 59, 3311
PsuI RGATCY 9 cut(s) 660, 1267, 1480, 2069, 3307, 3338, 3908, 4567, 4592
PteI GCGCGC 1 cut(s) 2318
RsaI GTAC 9 cut(s) 158, 359, 706, 812, 1421, 3580, 3597, 3604, 3788
RsaNI GTAC 9 cut(s) 157, 358, 705, 811, 1420, 3579, 3596, 3603, 3787
RseI CAYNNNNRTG 4 cut(s) 333, 344, 3716, 4466
SacI GAGCTC 1 cut(s) 3174
SapI GCTCTTC 2 cut(s) 936, 2688
Sau96I GGNCC 3 cut(s) 2461, 3843, 3918
SduI GDGCHC 6 cut(s) 2386, 3069, 3174, 3625, 4399, 4535
SfaNI GCATC 8 cut(s) 61, 91, 492, 940, 1183, 2170, 2207, 4353
SfcI CTRYAG 4 cut(s) 1071, 2945, 3249, 3736
SfuI TTCGAA 2 cut(s) 1314, 3471
SinI GGWCC 1 cut(s) 3843
SmiMI CAYNNNNRTG 4 cut(s) 333, 344, 3716, 4466
SmlI CTYRAG 5 cut(s) 2657, 2728, 3113, 3608, 4083
SmoI CTYRAG 5 cut(s) 2657, 2728, 3113, 3608, 4083
SnaBI TACGTA 1 cut(s) 1705
SpeI ACTAGT 1 cut(s) 3292
SphI GCATGC 1 cut(s) 2091
SseBI AGGCCT 1 cut(s) 3421
SsiI CCGC 6 cut(s) 302, 389, 785, 2035, 2932, 4445
SspMI CTAG 4 cut(s) 818, 2915, 3293, 3330
SstI GAGCTC 1 cut(s) 3174
StuI AGGCCT 1 cut(s) 3421
StyI CCWWGG 1 cut(s) 2493
TaiI ACGT 9 cut(s) 730, 1707, 1941, 2377, 2383, 3643, 4419, 4497, 4608
TaqII GACCGA 1 cut(s) 3507
TatI WGTACW 4 cut(s) 357, 1419, 3595, 3602
TauI GCSGC 2 cut(s) 788, 2934
TfiI GAWTC 9 cut(s) 50, 307, 1022, 3189, 3218, 3629, 4045, 4451, 4499
TseFI GTSAC 4 cut(s) 1132, 1660, 2960, 3851
Tsp45I GTSAC 4 cut(s) 1132, 1660, 2960, 3851
TspGWI ACGGA 4 cut(s) 2082, 2351, 3362, 3839
Van91I CCANNNNNTGG 1 cut(s) 349
Vha464I CTTAAG 1 cut(s) 2657
VneI GTGCAC 3 cut(s) 3065, 3621, 4531
VpaK11BI GGWCC 1 cut(s) 3843
XceI RCATGY 8 cut(s) 38, 148, 778, 1397, 1533, 2091, 2677, 4471
XcmI CCANNNNNNNNNTGG 4 cut(s) 589, 913, 961, 1955
XmiI GTMKAC 3 cut(s) 1212, 2365, 2377
XmnI GAANNNNTTC 1 cut(s) 311
XspI CTAG 4 cut(s) 818, 2915, 3293, 3330
Zsp2I ATGCAT 2 cut(s) 2093, 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.