MD03G1000600.v1.1

TATA-binding protein-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028498
Physical Location & Seq
Forward (+)
33794 .. 40446
6653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1000600.v1.1.491

Sequence Viewer

Length: 951 bp
ATGGCCCAGCAGTCCTCTCGTCTCCACCGCCTCCTCACTCTCTTGGACACTGGTTCAACCCAAGCGACCAGATTTACAGCTGCTCGGCAGATAGGGGACATTGCCAAAGCGCATCCTCAGGACTTGAGCTCTCTGTTGAAAAAGGTCTCCCAATATCTTCGTAGCAAAAACTGGGATACAAGGGTTGCTGCCGCTCATGCTGTTGGGGCTATTGCTGAGAATGTGAAGCACACCTCTTTGATTGAACTCTTTACTTGTATTAAATCGAAGGTGGCTGATGCTGGGATATCTGGTTCTATTGAAGATATGGTTGCCTTCCCTATTTTTGATTCTAACGTTGCTGGCACGTCATTTAGAAGCTTTGATCTCAAAAAGGTTTTGGAGTTTGGTGCTCTGTTTGCATCTGGGGGACAGGAGTATGATGTTGCAAATGATCATATGAAGAGCCCGAGGGAGAAACTGGCTCGACAGAAACAAACACTTCGACGTCGTTTAGGTTTGGATATATGTGAGCGGTTCATGGATGTGAATGATATGATAAAAGATGAAGACCTTATTTTACACAACTCGCATGGGAATGGGACTAATCCAAGAGTTTATACATCACATAGTATTCAGCAATTAGTTGCAAATATGGTTCCTAGTGTCTTATCAAAAAGGCCAAGTCCCAGGGAATTGAATCTTTTAAAACGAAAAGCAAAAATCACTTCGAAAGACCAGTCAAAAGGTTGGTCTGAGGATGGGGATGTGGAGGTGTCATGTGCTCAAAATATACCACCAAAGGGGGCATGTCCTGACTCATTCGGGACTAATAAGGAATTTTTGGACCTTGATAATCACGAAGAAAAAGTTGAACATGATGGAGAAGGGCGGTGGCCTTTTCATAGCTTCGTTGAGGAACTAATCCTAGATATGTTTGATCCAGGTTGGCTTCTATTATTTTATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0048509 GO:0048518 GO:0050789 GO:0050793 GO:0051094 GO:0065007 GO:1902183 GO:1902185
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3535 HEAT HEAT_EZ Helicase_C SNF2_N

Protein Analysis

317

Amino Acids

35.39

Weight (kDa)

6.6

Isoelectric Point (pI)

46.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028498)

Species Orthologous Gene IDs
malus_domestica MD03G1000600.v1.1
pyrus_communis pycom03g00090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 490
AccBSI CCGCTC 2 cut(s) 194, 514
AciI CCGC 4 cut(s) 28, 192, 514, 871
AclI AACGTT 1 cut(s) 336
AclWI GGATC 1 cut(s) 914
AcsI RAATTY 1 cut(s) 818
AcyI GRCGYC 1 cut(s) 487
AfiI CCNNNNNNNGG 1 cut(s) 782
AgsI TTSAA 6 cut(s) 57, 139, 245, 302, 679, 854
AjiI CACGTC 1 cut(s) 348
AjnI CCWGG 2 cut(s) 668, 922
AloI GAACNNNNNNTCC 2 cut(s) 277, 309
AluBI AGCT 4 cut(s) 80, 129, 360, 888
AluI AGCT 4 cut(s) 80, 129, 360, 888
Alw21I GWGCWC 3 cut(s) 131, 394, 766
Alw26I GTCTC 2 cut(s) 26, 151
AlwI GGATC 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 448
AoxI GGCC 3 cut(s) 3, 659, 875
ApeKI GCWGC 2 cut(s) 80, 188
ApoI RAATTY 1 cut(s) 818
ArsI GACNNNNNNTTYG 4 cut(s) 704, 716, 736, 748
AspLEI GCGC 1 cut(s) 112
AspS9I GGNCC 2 cut(s) 4, 826
AsuII TTCGAA 1 cut(s) 710
AvaI CYCGRG 1 cut(s) 448
AvaII GGWCC 1 cut(s) 826
AxyI CCTNAGG 1 cut(s) 117
BaeI ACNNNNGTAYC 2 cut(s) 168, 201
BanII GRGCYC 2 cut(s) 131, 449
BbsI GAAGAC 1 cut(s) 555
Bbv12I GWGCWC 3 cut(s) 131, 394, 766
BbvI GCAGC 2 cut(s) 67, 175
BccI CCATC 2 cut(s) 734, 854
BcgI CGANNNNNNTGC 1 cut(s) 33
BciT130I CCWGG 2 cut(s) 670, 924
BciVI GTATCC 1 cut(s) 169
BclI TGATCA 1 cut(s) 433
BcoDI GTCTC 2 cut(s) 26, 151
BfaI CTAG 3 cut(s) 642, 908, 949
BfuI GTATCC 1 cut(s) 169
BisI GCNGC 3 cut(s) 81, 189, 192
BlsI GCNGC 3 cut(s) 82, 190, 193
Bme1390I CCNGG 2 cut(s) 670, 924
Bme18I GGWCC 1 cut(s) 826
BmeT110I CYCGRG 1 cut(s) 448
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 2 cut(s) 4, 826
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 2 cut(s) 670, 924
BmrI ACTGGG 1 cut(s) 181
BmsI GCATC 3 cut(s) 121, 268, 410
BmuI ACTGGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 555
Bpu14I TTCGAA 1 cut(s) 710
BpuEI CTTGAG 1 cut(s) 145
BsaHI GRCGYC 1 cut(s) 487
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 3 cut(s) 449, 668, 669
Bsc4I CCNNNNNNNGG 1 cut(s) 782
Bse1I ACTGG 4 cut(s) 55, 176, 465, 718
Bse21I CCTNAGG 1 cut(s) 117
Bse3DI GCAATG 1 cut(s) 99
BseBI CCWGG 2 cut(s) 670, 924
BseDI CCNNGG 3 cut(s) 449, 668, 669
BseGI GGATG 4 cut(s) 112, 529, 745, 751
BseLI CCNNNNNNNGG 1 cut(s) 782
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 3 cut(s) 131, 207, 726
BseNI ACTGG 4 cut(s) 55, 176, 465, 718
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 2 cut(s) 67, 175
BseYI CCCAGC 2 cut(s) 6, 281
BshFI GGCC 3 cut(s) 5, 661, 877
BsiHKAI GWGCWC 3 cut(s) 131, 394, 766
BsiHKCI CYCGRG 1 cut(s) 448
BslFI GGGAC 5 cut(s) 110, 423, 595, 651, 820
BslI CCNNNNNNNGG 1 cut(s) 782
BsmAI GTCTC 2 cut(s) 26, 151
BsmBI CGTCTC 1 cut(s) 26
BsmFI GGGAC 5 cut(s) 110, 423, 595, 651, 820
BsnI GGCC 3 cut(s) 5, 661, 877
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 1 cut(s) 448
Bsp119I TTCGAA 1 cut(s) 710
Bsp1286I GDGCHC 4 cut(s) 131, 394, 449, 766
Bsp143I GATC 3 cut(s) 364, 433, 919
BspACI CCGC 4 cut(s) 28, 192, 514, 871
BspANI GGCC 3 cut(s) 5, 661, 877
BspCNI CTCAG 3 cut(s) 130, 208, 727
BspLI GGNNCC 1 cut(s) 639
BspPI GGATC 1 cut(s) 914
BspQI GCTCTTC 1 cut(s) 437
BspT104I TTCGAA 1 cut(s) 710
BspTNI GGTCTC 1 cut(s) 151
BsrBI CCGCTC 2 cut(s) 194, 514
BsrDI GCAATG 1 cut(s) 99
BsrI ACTGG 4 cut(s) 55, 176, 465, 718
BssECI CCNNGG 3 cut(s) 449, 668, 669
BssMI GATC 3 cut(s) 364, 433, 919
BssNI GRCGYC 1 cut(s) 487
Bst2UI CCWGG 2 cut(s) 670, 924
Bst6I CTCTTC 1 cut(s) 437
BstACI GRCGYC 1 cut(s) 487
BstBI TTCGAA 1 cut(s) 710
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 3 cut(s) 117, 216, 735
BstF5I GGATG 4 cut(s) 112, 529, 745, 751
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 3 cut(s) 367, 436, 922
BstMAI GTCTC 2 cut(s) 26, 151
BstMBI GATC 3 cut(s) 364, 433, 919
BstMWI GCNNNNNNNGC 3 cut(s) 197, 206, 398
BstNI CCWGG 2 cut(s) 670, 924
BstNSI RCATGY 1 cut(s) 792
BstSCI CCNGG 2 cut(s) 668, 922
BstV1I GCAGC 2 cut(s) 67, 175
BstV2I GAAGAC 1 cut(s) 555
Bsu36I CCTNAGG 1 cut(s) 117
BsuI GTATCC 1 cut(s) 169
BsuRI GGCC 3 cut(s) 5, 661, 877
BtrI CACGTC 1 cut(s) 348
BtsCI GGATG 4 cut(s) 112, 529, 745, 751
BtsIMutI CAGTG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 112
Cfr13I GGNCC 2 cut(s) 4, 826
CviAII CATG 6 cut(s) 197, 520, 572, 759, 789, 857
DdeI CTNAG 3 cut(s) 117, 216, 735
DpnI GATC 3 cut(s) 366, 435, 921
DpnII GATC 3 cut(s) 364, 433, 919
DraI TTTAAA 1 cut(s) 687
Eam1104I CTCTTC 1 cut(s) 437
EarI CTCTTC 1 cut(s) 437
Ecl136II GAGCTC 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 131, 449
Eco31I GGTCTC 1 cut(s) 151
Eco32I GATATC 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 129
Eco81I CCTNAGG 1 cut(s) 117
Eco88I CYCGRG 1 cut(s) 448
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 2 cut(s) 668, 922
EcoRV GATATC 1 cut(s) 288
EcoT38I GRGCYC 2 cut(s) 131, 449
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 6 cut(s) 200, 523, 575, 762, 792, 860
FaqI GGGAC 5 cut(s) 110, 423, 595, 651, 820
FatI CATG 6 cut(s) 196, 519, 571, 758, 788, 856
FauNDI CATATG 1 cut(s) 438
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 3 cut(s) 81, 189, 192
FokI GGATG 4 cut(s) 99, 536, 752, 758
FriOI GRGCYC 2 cut(s) 131, 449
Fsp4HI GCNGC 3 cut(s) 81, 189, 192
FspBI CTAG 3 cut(s) 642, 908, 949
GlaI GCGC 1 cut(s) 111
GluI GCNGC 3 cut(s) 81, 189, 192
GsaI CCCAGC 2 cut(s) 10, 285
HaeIII GGCC 3 cut(s) 5, 661, 877
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 1 cut(s) 487
Hin1II CATG 6 cut(s) 200, 523, 575, 762, 792, 860
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HindIII AAGCTT 1 cut(s) 358
HinfI GANTC 3 cut(s) 329, 679, 797
Hpy188I TCNGA 1 cut(s) 736
Hpy188III TCNNGA 4 cut(s) 119, 794, 805, 839
Hpy99I CGWCG 2 cut(s) 489, 492
HpyAV CCTTC 3 cut(s) 262, 325, 860
HpyCH4IV ACGT 3 cut(s) 336, 347, 487
HpyCH4V TGCA 3 cut(s) 401, 428, 629
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 206, 398
HpyF3I CTNAG 3 cut(s) 117, 216, 735
HpySE526I ACGT 3 cut(s) 336, 347, 487
Hsp92I GRCGYC 1 cut(s) 487
Hsp92II CATG 6 cut(s) 200, 523, 575, 762, 792, 860
HspAI GCGC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 3 cut(s) 364, 433, 919
LguI GCTCTTC 1 cut(s) 437
Lsp1109I GCAGC 2 cut(s) 67, 175
LweI GCATC 3 cut(s) 121, 268, 410
MaeI CTAG 3 cut(s) 642, 908, 949
MaeII ACGT 3 cut(s) 336, 347, 487
MalI GATC 3 cut(s) 366, 435, 921
MbiI CCGCTC 2 cut(s) 194, 514
MboI GATC 3 cut(s) 364, 433, 919
MboII GAAGA 5 cut(s) 149, 314, 454, 560, 854
MhlI GDGCHC 4 cut(s) 131, 394, 449, 766
MluCI AATT 3 cut(s) 620, 674, 818
MlyI GAGTC 1 cut(s) 791
MnlI CCTC 9 cut(s) 25, 41, 44, 126, 244, 444, 730, 745, 889
MseI TTAA 2 cut(s) 261, 686
MslI CAYNNNNRTG 2 cut(s) 524, 576
MspA1I CMGCKG 1 cut(s) 80
MspR9I CCNGG 2 cut(s) 670, 924
MvaI CCWGG 2 cut(s) 670, 924
MwoI GCNNNNNNNGC 3 cut(s) 197, 206, 398
NdeI CATATG 1 cut(s) 438
NdeII GATC 3 cut(s) 364, 433, 919
NlaIII CATG 6 cut(s) 200, 523, 575, 762, 792, 860
NlaIV GGNNCC 1 cut(s) 639
NmeAIII GCCGAG 1 cut(s) 64
NspI RCATGY 1 cut(s) 792
NspV TTCGAA 1 cut(s) 710
PasI CCCWGGG 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 437
PfeI GAWTC 2 cut(s) 329, 679
PkrI GCNGC 3 cut(s) 82, 190, 193
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 336
Psp6I CCWGG 2 cut(s) 668, 922
PspFI CCCAGC 2 cut(s) 6, 281
PspGI CCWGG 2 cut(s) 668, 922
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 2 cut(s) 4, 826
PvuII CAGCTG 1 cut(s) 80
RseI CAYNNNNRTG 2 cut(s) 524, 576
SacI GAGCTC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 261, 686
SatI GCNGC 3 cut(s) 81, 189, 192
Sau3AI GATC 3 cut(s) 364, 433, 919
Sau96I GGNCC 2 cut(s) 4, 826
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 2 cut(s) 670, 924
SduI GDGCHC 4 cut(s) 131, 394, 449, 766
SfaNI GCATC 3 cut(s) 121, 268, 410
SfuI TTCGAA 1 cut(s) 710
SinI GGWCC 1 cut(s) 826
SmiMI CAYNNNNRTG 2 cut(s) 524, 576
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 3 cut(s) 620, 674, 818
SsiI CCGC 4 cut(s) 28, 192, 514, 871
SspMI CTAG 3 cut(s) 642, 908, 949
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 668, 922
TaiI ACGT 3 cut(s) 339, 350, 490
TaqI TCGA 4 cut(s) 266, 466, 484, 710
TasI AATT 3 cut(s) 620, 674, 818
TauI GCSGC 1 cut(s) 194
TfiI GAWTC 2 cut(s) 329, 679
Tru1I TTAA 2 cut(s) 261, 686
Tru9I TTAA 2 cut(s) 261, 686
TscAI CASTG 1 cut(s) 55
TseI GCWGC 2 cut(s) 80, 188
TspDTI ATGAA 4 cut(s) 455, 508, 561, 872
TspRI CASTG 1 cut(s) 55
VpaK11BI GGWCC 1 cut(s) 826
XapI RAATTY 1 cut(s) 818
XceI RCATGY 1 cut(s) 792
XspI CTAG 3 cut(s) 642, 908, 949
ZraI GACGTC 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.