MD03G1041200.v1.1

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028496
Physical Location & Seq
Forward (+)
3275199 .. 3277477
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1041200.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGAAATGACGTCATCATCGGGCGGTGGAAGGTACATGGCGTACTCGCCGTCACCGTCGGCACCTCACTCCCCTCACCTCTCCGGCCTTCGCTCCGCCTCCTCCTCCGCTGCTCTCGTTGCCGCCGAGAAAGAAAAATATCTATCTGAGCTGCTTGGGGAGCGTCACAAGCTGAGTCCATTTTTGCCTGTGCTGCCGCATTGCAACAGGTTACTAAACCAAGGTTTAAGTGGTTTGGTGATATCGTCTCAAGCCTACTGGTGGTGTTTGGTGGATGTGTATCCTCTCTTGGGGTTTCAAATATTGCGTGTAACTACACTGTTGGGGAATGCGTCAGTTTTAGGTCAAAGTGGGCTTGAACATGCTAGCCCCCTGGCATCTAGAGGAATGTTTCCAAATGGAGGTGCTGATGTGAACGGATGGGCATCACGGATTCAATCAGAAGTACGGATACTAACCATTGACTTATTAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1 STAR_dimer

Protein Analysis

159

Amino Acids

16.89

Weight (kDa)

7.86

Isoelectric Point (pI)

60.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
STAR_dimer PF16544 44 - 74 6.4e-07 Homodimerisation region of STAR domain protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028496)

Species Orthologous Gene IDs
malus_domestica MD03G1041200.v1.1
pyrus_communis pycom11g03640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 14
AccB1I GGYRCC 1 cut(s) 61
AciI CCGC 5 cut(s) 24, 96, 108, 123, 197
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 3 cut(s) 35, 44, 447
AfiI CCNNNNNNNGG 3 cut(s) 261, 290, 401
AgsI TTSAA 3 cut(s) 299, 359, 437
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 2 cut(s) 151, 172
AluI AGCT 2 cut(s) 151, 172
Alw26I GTCTC 1 cut(s) 252
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 110, 151, 193
AsuHPI GGTGA 3 cut(s) 45, 68, 250
AsuNHI GCTAGC 1 cut(s) 365
BanI GGYRCC 1 cut(s) 61
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
BbvI GCAGC 3 cut(s) 97, 138, 180
BccI CCATC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 374
BciVI GTATCC 2 cut(s) 291, 444
BcoDI GTCTC 1 cut(s) 252
BfaI CTAG 2 cut(s) 366, 381
BfuI GTATCC 2 cut(s) 291, 444
BisI GCNGC 5 cut(s) 111, 123, 152, 194, 197
BlsI GCNGC 5 cut(s) 112, 124, 153, 195, 198
Bme1390I CCNGG 1 cut(s) 374
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 386, 434
BmtI GCTAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 234
BsaBI GATNNNNATC 2 cut(s) 279, 424
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 220, 372
Bsc4I CCNNNNNNNGG 3 cut(s) 261, 290, 401
Bse1I ACTGG 1 cut(s) 263
Bse3DI GCAATG 1 cut(s) 199
Bse8I GATNNNNATC 2 cut(s) 279, 424
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 2 cut(s) 220, 372
BseGI GGATG 2 cut(s) 280, 425
BseJI GATNNNNATC 2 cut(s) 279, 424
BseLI CCNNNNNNNGG 3 cut(s) 261, 290, 401
BseMI GCAATG 1 cut(s) 199
BseMII CTCAG 2 cut(s) 138, 164
BseNI ACTGG 1 cut(s) 263
BseRI GAGGAG 2 cut(s) 91, 94
BseXI GCAGC 3 cut(s) 97, 138, 180
BshFI GGCC 1 cut(s) 87
BshNI GGYRCC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 84
BslI CCNNNNNNNGG 3 cut(s) 261, 290, 401
BsmAI GTCTC 1 cut(s) 252
BsmBI CGTCTC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 334
BsnI GGCC 1 cut(s) 87
BspACI CCGC 5 cut(s) 24, 96, 108, 123, 197
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 2 cut(s) 139, 165
BspLI GGNNCC 1 cut(s) 63
BspOI GCTAGC 1 cut(s) 369
BspT107I GGYRCC 1 cut(s) 61
BsrDI GCAATG 1 cut(s) 199
BsrI ACTGG 1 cut(s) 263
BssECI CCNNGG 2 cut(s) 220, 372
BssNI GRCGYC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 220
Bst2UI CCWGG 1 cut(s) 374
Bst4CI ACNGT 2 cut(s) 57, 321
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 2 cut(s) 147, 173
BstF5I GGATG 2 cut(s) 280, 425
BstMAI GTCTC 1 cut(s) 252
BstMWI GCNNNNNNNGC 4 cut(s) 119, 160, 169, 193
BstNI CCWGG 1 cut(s) 374
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 372
BstV1I GCAGC 3 cut(s) 97, 138, 180
BsuI GTATCC 2 cut(s) 291, 444
BsuRI GGCC 1 cut(s) 87
BtsCI GGATG 2 cut(s) 280, 425
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 1 cut(s) 367
CseI GACGC 2 cut(s) 152, 321
Csp6I GTAC 3 cut(s) 34, 43, 446
CviAII CATG 2 cut(s) 37, 362
CviJI RGCY 6 cut(s) 87, 151, 172, 254, 355, 369
CviKI_1 RGCY 6 cut(s) 87, 151, 172, 254, 355, 369
CviQI GTAC 3 cut(s) 34, 43, 446
DdeI CTNAG 2 cut(s) 147, 173
EciI GGCGGA 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 220
Eco32I GATATC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 372
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 220
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 2 cut(s) 40, 365
FaiI YATR 2 cut(s) 38, 363
FatI CATG 2 cut(s) 36, 361
Fnu4HI GCNGC 5 cut(s) 111, 123, 152, 194, 197
FokI GGATG 2 cut(s) 287, 432
Fsp4HI GCNGC 5 cut(s) 111, 123, 152, 194, 197
FspBI CTAG 2 cut(s) 366, 381
GluI GCNGC 5 cut(s) 111, 123, 152, 194, 197
HaeIII GGCC 1 cut(s) 87
HapII CCGG 1 cut(s) 84
HgaI GACGC 2 cut(s) 152, 321
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 2 cut(s) 40, 365
HinfI GANTC 2 cut(s) 175, 433
HpaII CCGG 1 cut(s) 84
HphI GGTGA 3 cut(s) 45, 68, 250
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 2 cut(s) 148, 442
Hpy188III TCNNGA 1 cut(s) 381
Hpy8I GTNNAC 1 cut(s) 415
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 24, 98
HpyCH4III ACNGT 2 cut(s) 57, 321
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 160, 169, 193
HpyF3I CTNAG 2 cut(s) 147, 173
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 2 cut(s) 40, 365
LmnI GCTCC 2 cut(s) 98, 160
LpnPI CCDG 6 cut(s) 97, 193, 201, 244, 359, 386
Lsp1109I GCAGC 3 cut(s) 97, 138, 180
LweI GCATC 2 cut(s) 386, 434
MaeI CTAG 2 cut(s) 366, 381
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 4 cut(s) 51, 164, 210, 310
MlyI GAGTC 1 cut(s) 184
MnlI CCTC 9 cut(s) 75, 84, 89, 109, 112, 115, 294, 377, 395
MseI TTAA 3 cut(s) 227, 470, 475
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 1 cut(s) 84
MspR9I CCNGG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 334
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 4 cut(s) 119, 160, 169, 193
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 2 cut(s) 40, 365
NlaIV GGNNCC 1 cut(s) 63
NmeAIII GCCGAG 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 51, 164
NspI RCATGY 1 cut(s) 365
PcsI WCGNNNNNNNCGW 2 cut(s) 53, 123
PctI GAATGC 1 cut(s) 334
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 5 cut(s) 112, 124, 153, 195, 198
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspN4I GGNNCC 1 cut(s) 63
RsaI GTAC 3 cut(s) 35, 44, 447
RsaNI GTAC 3 cut(s) 34, 43, 446
SaqAI TTAA 3 cut(s) 227, 470, 475
SatI GCNGC 5 cut(s) 111, 123, 152, 194, 197
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 1 cut(s) 374
SfaNI GCATC 2 cut(s) 386, 434
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
SsiI CCGC 5 cut(s) 24, 96, 108, 123, 197
SspI AATATT 1 cut(s) 303
SspMI CTAG 2 cut(s) 366, 381
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 2 cut(s) 57, 321
TaiI ACGT 1 cut(s) 14
TauI GCSGC 2 cut(s) 125, 199
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 3 cut(s) 227, 470, 475
Tru9I TTAA 3 cut(s) 227, 470, 475
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 2 cut(s) 51, 164
TseI GCWGC 3 cut(s) 110, 151, 193
Tsp45I GTSAC 2 cut(s) 51, 164
TspGWI ACGGA 3 cut(s) 432, 445, 463
TspRI CASTG 1 cut(s) 324
XbaI TCTAGA 1 cut(s) 380
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XspI CTAG 2 cut(s) 366, 381
ZraI GACGTC 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.