MD03G1084500.v1.1

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028481
Physical Location & Seq
Reverse (-)
6815699 .. 6818546
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1084500.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGCTGGACATGAGCTTGTTATGCAAGGCAACTGATGAGGATAGAAAGGTTCATATTGTGTACATGGGGTCCCTTCCAGATGGGGAGTTCTCGCCGGTGTCTCACCACCTAAGTATTCTGCAGAGAGTTGTCCAGGGAAGTTCGCCGGAAAGTTTACTGGTACGAAGTTACAAAAGGAGTTTCAATGGGTTTGCAGCAAAACTCACTGACAAGGAAAGGGAAAAACTTGCTGTTGAGAGTGATATAATTGTTGGGGTGATTGACACTGGAATTTGGCCGGAATCTGAGAGCTTTAAAGACGAAGGTTTTGGTCCTCCTCCAAAGAAAGGTGCTTGCGAAGGAGGCAAAAATTTCACTTGCAACAAGTTCCCTCACTTCTCATCTCTCAGTTATTCCTATGGAAATGGTATTTCGTTCCTATTGGCAGCAACGGGCATGCTTTTGCCTTCGTGTCTTCTGACCAGTGTTCGTGGAGTGTGTATTCACCATTTGGCAGTTGTAGCATTGGTACTTGCCTCTGGTCCTCTGGGTATACTAAGATTATATGCAAGAGGAGGTGTTCCTGCAGCAGGAATTGCAACATATAAACTTTGTGATCATGATAAAGGGTGCAGTGCAGATTCCATCTTGGCTGCTTTTGACGATGCTATTGATGACGGAGTTAACGTCATTACGATTTCAATTGGATCTGGTTTTGTGACTAGTTTTGATGAGGACCCTTTCACAATTGGTGCTTTTCATGCAATGAAAAAAGGGATACTGACCATACAGCCTGCAGGCAATGAAGGGCCTCAAATCGCTACTGTGTCATGCGTAGCACCATGGATATTGACAGCAAGTACCATAGATCGCCATATCATTGACAAAGTTGTTCTGGCAAATGGAAAGACACTTGTTGGGAATTCTATTAACACTTTCAGATTAAATGGAACAAGTTTTCCCTCAATACACGGAAAAGATGCTGCAGCGAAAGATTGCACTCGACAAGATGCTGGATTTTGTAAATGTGGTTGCCTGGACAGCGATTCTGTTAAGGGAAAGATAGTGGTATGTGATGCCGGATACGGAGATACAACAGCATATGATTCCGGTGCACTTGGTTCAATTTTACATAATGACAACACAGACGATCTTAGTCAAGTTCCCATATTACCTACAACAATGTTAATGATGGAAGAGCATAATTTGGTCAAATCCTACATAAACTCCACCAGTGACCCTCGAGCGAAAATACTTAAAAGTGATGCCATAAAAGATCCTTCTGCACCTGCTGTCACTTCGTTCTCCTCGCGTGGACCAAATCTAATTTTGCCTGATATTATAAAGCCGGATATTAGTGTCCCAGGGGCTGAAATTCTGGCTACATATTCACCTAACGCTTCGATCACACAATTGGAAGAAGACAAGAGGCGTGTAAAATACAATATGTTGTCTGGAACCTCTGTATCTTGCCCTCATGCTGCTGGTGCAGCGGCATATGTTAAAGCATTCCACCCTGATTGGTTTCCCACCGCCATCAAATCATCTCTTATGACGACTGCTTGGCTAATGAATGATACCAGCAACAAAATTTCCACTGGTGCATTTGCTTATGGATCGGGACATATCAATCCTCTGAACGCTGTAACGCCGGGGCTTGTTTACGAAGCTTGTGAAGAAGATTACATAAAGTTGCTATGCATAATGTATGATGAAGGCAAAGTTAGACTCATGTCAGGAGATAACAGCACATGCCCTAAAGGATCTGAGAAAGGATATCCAAAGGATCTTAATCACCCTTCAATGGGAGTTAAAGTTGCAGAAAAGGAACCTTTTACAGTTAAATTTCACAGAAGAGTTAAAAATGTTGGCTTTCCAAACTCGACCTACAAGGCCAAAATATCGTCGGATTCCCGATTTGACAACTTGACATCAAAGTTGTTCCCGAAGTTCTGGCATTCGAGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

650

Amino Acids

69.69

Weight (kDa)

6.53

Isoelectric Point (pI)

39.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 17 - 82 5.5e-10 Peptidase inhibitor I9
Peptidase_S8 PF00082 162 - 532 8.1e-30 Subtilase family
fn3_6 PF17766 589 - 632 5.9e-07 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028481)

Species Orthologous Gene IDs
malus_domestica MD03G1084500.v1.1
pyrus_communis pycom03g06670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1322
AarI CACCTGC 1 cut(s) 1276
Acc36I ACCTGC 1 cut(s) 1276
AccI GTMKAC 1 cut(s) 532
AccII CGCG 1 cut(s) 1291
AciI CCGC 2 cut(s) 1472, 1512
AclWI GGATC 5 cut(s) 694, 1250, 1603, 1750, 1773
AcoI YGGCCR 1 cut(s) 275
AcsI RAATTY 6 cut(s) 270, 349, 901, 1353, 1569, 1823
AfaI GTAC 4 cut(s) 62, 162, 510, 841
AfiI CCNNNNNNNGG 4 cut(s) 326, 569, 1783, 1784
AgsI TTSAA 4 cut(s) 184, 681, 1104, 1782
AhlI ACTAGT 1 cut(s) 701
AjnI CCWGG 3 cut(s) 132, 1014, 1342
AloI GAACNNNNNNTCC 2 cut(s) 922, 954
AluBI AGCT 3 cut(s) 15, 291, 1649
AluI AGCT 3 cut(s) 15, 291, 1649
Alw21I GWGCWC 1 cut(s) 1096
Alw26I GTCTC 1 cut(s) 105
Alw44I GTGCAC 1 cut(s) 1092
AlwI GGATC 5 cut(s) 694, 1250, 1603, 1750, 1773
AlwNI CAGNNNCTG 1 cut(s) 1349
Ama87I CYCGRG 1 cut(s) 1221
AoxI GGCC 3 cut(s) 275, 788, 1872
ApaLI GTGCAC 1 cut(s) 1092
ApeKI GCWGC 8 cut(s) 194, 425, 566, 632, 962, 965, 1460, 1469
ApoI RAATTY 6 cut(s) 270, 349, 901, 1353, 1569, 1823
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AspS9I GGNCC 6 cut(s) 69, 311, 521, 715, 788, 1295
AsuC2I CCSGG 1 cut(s) 1632
AsuHPI GGTGA 5 cut(s) 95, 268, 476, 1362, 1766
AvaI CYCGRG 1 cut(s) 1221
AvaII GGWCC 5 cut(s) 69, 311, 521, 715, 1295
BaeGI GKGCMC 1 cut(s) 1096
BaeI ACNNNNGTAYC 2 cut(s) 1428, 1461
BbsI GAAGAC 2 cut(s) 446, 1407
Bbv12I GWGCWC 1 cut(s) 1096
BbvI GCAGC 8 cut(s) 206, 437, 578, 619, 949, 977, 1447, 1481
BccI CCATC 4 cut(s) 74, 632, 1165, 1523
BciT130I CCWGG 3 cut(s) 134, 1016, 1344
BciVI GTATCC 2 cut(s) 750, 1055
BclI TGATCA 1 cut(s) 595
BcnI CCSGG 1 cut(s) 1632
BcoDI GTCTC 1 cut(s) 105
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 1 cut(s) 702
BfmI CTRYAG 4 cut(s) 119, 564, 774, 963
BfuAI ACCTGC 1 cut(s) 1276
BfuI GTATCC 2 cut(s) 750, 1055
BisI GCNGC 9 cut(s) 195, 426, 567, 633, 963, 966, 1461, 1470, 1473
BlsI GCNGC 9 cut(s) 196, 427, 568, 634, 964, 967, 1462, 1471, 1474
Bme1390I CCNGG 4 cut(s) 134, 1016, 1344, 1632
Bme18I GGWCC 5 cut(s) 69, 311, 521, 715, 1295
BmeT110I CYCGRG 1 cut(s) 1221
BmgT120I GGNCC 6 cut(s) 69, 311, 521, 715, 788, 1295
BmiI GGNNCC 5 cut(s) 70, 71, 717, 1438, 1809
BmrFI CCNGG 4 cut(s) 134, 1016, 1344, 1632
BmsI GCATC 5 cut(s) 634, 949, 979, 1045, 1234
BoxI GACNNNNGTC 1 cut(s) 1711
BpiI GAAGAC 2 cut(s) 446, 1407
BpuMI CCSGG 1 cut(s) 1632
BsaBI GATNNNNATC 1 cut(s) 1770
BsaJI CCNNGG 5 cut(s) 133, 821, 1342, 1343, 1631
BsaWI WCCGGW 1 cut(s) 1088
BsaXI ACNNNNNCTCC 6 cut(s) 651, 681, 1190, 1220, 1779, 1809
Bsc4I CCNNNNNNNGG 4 cut(s) 326, 569, 1783, 1784
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 5 cut(s) 162, 271, 462, 1212, 1582
Bse3DI GCAATG 2 cut(s) 750, 787
Bse8I GATNNNNATC 1 cut(s) 1770
BseBI CCWGG 3 cut(s) 134, 1016, 1344
BseDI CCNNGG 5 cut(s) 133, 821, 1342, 1343, 1631
BseJI GATNNNNATC 1 cut(s) 1770
BseLI CCNNNNNNNGG 4 cut(s) 326, 569, 1783, 1784
BseMI GCAATG 2 cut(s) 750, 787
BseMII CTCAG 3 cut(s) 276, 400, 1737
BseNI ACTGG 5 cut(s) 162, 271, 462, 1212, 1582
BseRI GAGGAG 3 cut(s) 306, 567, 1276
BseSI GKGCMC 1 cut(s) 1096
BseXI GCAGC 8 cut(s) 206, 437, 578, 619, 949, 977, 1447, 1481
BsgI GTGCAG 4 cut(s) 631, 636, 1248, 1488
Bsh1236I CGCG 1 cut(s) 1291
BshFI GGCC 3 cut(s) 277, 790, 1874
BsiHKAI GWGCWC 1 cut(s) 1096
BsiHKCI CYCGRG 1 cut(s) 1221
BsiSI CCGG 7 cut(s) 95, 146, 278, 1059, 1089, 1328, 1631
BslFI GGGAC 3 cut(s) 55, 1325, 1614
BslI CCNNNNNNNGG 4 cut(s) 326, 569, 1783, 1784
BsmAI GTCTC 1 cut(s) 105
BsmFI GGGAC 3 cut(s) 55, 1325, 1614
BsmI GAATGC 2 cut(s) 1487, 1936
BsnI GGCC 3 cut(s) 277, 790, 1874
BsoBI CYCGRG 1 cut(s) 1221
Bsp1286I GDGCHC 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
Bsp19I CCATGG 1 cut(s) 821
BspACI CCGC 2 cut(s) 1472, 1512
BspANI GGCC 3 cut(s) 277, 790, 1874
BspCNI CTCAG 3 cut(s) 277, 399, 1738
BspFNI CGCG 1 cut(s) 1291
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 5 cut(s) 70, 71, 717, 1438, 1809
BspMAI CTGCAG 4 cut(s) 123, 568, 778, 967
BspMI ACCTGC 1 cut(s) 1276
BspPI GGATC 5 cut(s) 694, 1250, 1603, 1750, 1773
BspQI GCTCTTC 1 cut(s) 1170
BsrDI GCAATG 2 cut(s) 750, 787
BsrFI RCCGGY 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 5 cut(s) 162, 271, 462, 1212, 1582
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 5 cut(s) 133, 821, 1342, 1343, 1631
BssMI GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
BssNAI GTATAC 1 cut(s) 533
BssT1I CCWWGG 1 cut(s) 821
Bst1107I GTATAC 1 cut(s) 533
Bst2UI CCWGG 3 cut(s) 134, 1016, 1344
Bst4CI ACNGT 2 cut(s) 805, 1819
Bst6I CTCTTC 2 cut(s) 1170, 1828
BstAPI GCANNNNNTGC 1 cut(s) 575
BstAUI TGTACA 1 cut(s) 60
BstC8I GCNNGC 4 cut(s) 334, 437, 774, 778
BstDEI CTNAG 6 cut(s) 110, 285, 386, 536, 1133, 1746
BstDSI CCRYGG 1 cut(s) 821
BstENI CCTNNNNNAGG 1 cut(s) 567
BstFNI CGCG 1 cut(s) 1291
BstKTI GATC 9 cut(s) 598, 689, 850, 1132, 1258, 1386, 1598, 1745, 1768
BstMAI GTCTC 1 cut(s) 105
BstMBI GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
BstMWI GCNNNNNNNGC 8 cut(s) 21, 342, 500, 575, 740, 1020, 1466, 1469
BstNI CCWGG 3 cut(s) 134, 1016, 1344
BstNSI RCATGY 2 cut(s) 439, 1734
BstPAI GACNNNNGTC 1 cut(s) 1711
BstSCI CCNGG 4 cut(s) 132, 1014, 1342, 1630
BstSFI CTRYAG 4 cut(s) 119, 564, 774, 963
BstSLI GKGCMC 1 cut(s) 1096
BstUI CGCG 1 cut(s) 1291
BstV1I GCAGC 8 cut(s) 206, 437, 578, 619, 949, 977, 1447, 1481
BstV2I GAAGAC 2 cut(s) 446, 1407
BstX2I RGATCY 4 cut(s) 686, 1255, 1742, 1765
BstYI RGATCY 4 cut(s) 686, 1255, 1742, 1765
BstZ17I GTATAC 1 cut(s) 533
BsuI GTATCC 2 cut(s) 750, 1055
BsuRI GGCC 3 cut(s) 277, 790, 1874
BtgI CCRYGG 1 cut(s) 821
BtsI GCAGTG 1 cut(s) 619
BtsIMutI CAGTG 6 cut(s) 204, 264, 469, 619, 1219, 1575
BveI ACCTGC 1 cut(s) 1276
Cac8I GCNNGC 4 cut(s) 334, 437, 774, 778
CaiI CAGNNNCTG 1 cut(s) 1349
CciI TCATGA 1 cut(s) 598
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 6 cut(s) 69, 311, 521, 715, 788, 1295
Csp6I GTAC 4 cut(s) 61, 161, 509, 840
CspCI CAANNNNNGTGG 2 cut(s) 1481, 1516
CviQI GTAC 4 cut(s) 61, 161, 509, 840
DdeI CTNAG 6 cut(s) 110, 285, 386, 536, 1133, 1746
DpnI GATC 9 cut(s) 597, 688, 849, 1131, 1257, 1385, 1597, 1744, 1767
DpnII GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
DraI TTTAAA 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 275
Eam1104I CTCTTC 2 cut(s) 1170, 1828
EarI CTCTTC 2 cut(s) 1170, 1828
Eco130I CCWWGG 1 cut(s) 821
Eco32I GATATC 1 cut(s) 1757
Eco47I GGWCC 5 cut(s) 69, 311, 521, 715, 1295
Eco88I CYCGRG 1 cut(s) 1221
EcoNI CCTNNNNNAGG 1 cut(s) 567
EcoO109I RGGNCCY 3 cut(s) 69, 715, 788
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 3 cut(s) 132, 1014, 1342
EcoRV GATATC 1 cut(s) 1757
EcoT14I CCWWGG 1 cut(s) 821
EcoT22I ATGCAT 1 cut(s) 1682
ErhI CCWWGG 1 cut(s) 821
FaqI GGGAC 3 cut(s) 55, 1325, 1614
FauNDI CATATG 2 cut(s) 1081, 1477
FbaI TGATCA 1 cut(s) 595
FblI GTMKAC 1 cut(s) 532
Fnu4HI GCNGC 9 cut(s) 195, 426, 567, 633, 963, 966, 1461, 1470, 1473
Fsp4HI GCNGC 9 cut(s) 195, 426, 567, 633, 963, 966, 1461, 1470, 1473
FspBI CTAG 1 cut(s) 702
GluI GCNGC 9 cut(s) 195, 426, 567, 633, 963, 966, 1461, 1470, 1473
HaeIII GGCC 3 cut(s) 277, 790, 1874
HapII CCGG 7 cut(s) 95, 146, 278, 1059, 1089, 1328, 1631
HincII GTYRAC 1 cut(s) 664
HindII GTYRAC 1 cut(s) 664
HindIII AAGCTT 1 cut(s) 1647
HinfI GANTC 7 cut(s) 281, 620, 1025, 1085, 1707, 1889, 1942
HpaI GTTAAC 1 cut(s) 664
HpaII CCGG 7 cut(s) 95, 146, 278, 1059, 1089, 1328, 1631
HphI GGTGA 5 cut(s) 95, 268, 476, 1362, 1766
Hpy166II GTNNAC 7 cut(s) 61, 155, 533, 664, 1094, 1295, 1642
Hpy188I TCNGA 7 cut(s) 286, 459, 920, 1617, 1747, 1888, 1949
Hpy188III TCNNGA 7 cut(s) 77, 599, 1434, 1599, 1716, 1893, 1924
Hpy8I GTNNAC 7 cut(s) 61, 155, 533, 664, 1094, 1295, 1642
Hpy99I CGWCG 1 cut(s) 1888
HpyAV CCTTC 8 cut(s) 83, 296, 332, 456, 779, 1269, 1688, 1788
HpyCH4III ACNGT 2 cut(s) 805, 1819
HpyCH4IV ACGT 1 cut(s) 666
HpyF10VI GCNNNNNNNGC 8 cut(s) 21, 342, 500, 575, 740, 1020, 1466, 1469
HpyF3I CTNAG 6 cut(s) 110, 285, 386, 536, 1133, 1746
HpySE526I ACGT 1 cut(s) 666
KflI GGGWCCC 1 cut(s) 69
Ksp22I TGATCA 1 cut(s) 595
KspAI GTTAAC 1 cut(s) 664
Kzo9I GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
LguI GCTCTTC 1 cut(s) 1170
Lsp1109I GCAGC 8 cut(s) 206, 437, 578, 619, 949, 977, 1447, 1481
LweI GCATC 5 cut(s) 634, 949, 979, 1045, 1234
MaeI CTAG 1 cut(s) 702
MaeII ACGT 1 cut(s) 666
MaeIII GTNAC 5 cut(s) 167, 697, 1214, 1273, 1624
MalI GATC 9 cut(s) 597, 688, 849, 1131, 1257, 1385, 1597, 1744, 1767
MboI GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
MboII GAAGA 7 cut(s) 446, 1187, 1409, 1412, 1667, 1670, 1845
MfeI CAATTG 3 cut(s) 681, 726, 1391
MflI RGATCY 4 cut(s) 686, 1255, 1742, 1765
MhlI GDGCHC 1 cut(s) 1096
MlyI GAGTC 1 cut(s) 1701
MmeI TCCRAC 1 cut(s) 1866
Mph1103I ATGCAT 1 cut(s) 1682
MspA1I CMGCKG 1 cut(s) 1472
MspI CCGG 7 cut(s) 95, 146, 278, 1059, 1089, 1328, 1631
MspR9I CCNGG 4 cut(s) 134, 1016, 1344, 1632
MunI CAATTG 3 cut(s) 681, 726, 1391
Mva1269I GAATGC 2 cut(s) 1487, 1936
MvaI CCWGG 3 cut(s) 134, 1016, 1344
MvnI CGCG 1 cut(s) 1291
MwoI GCNNNNNNNGC 8 cut(s) 21, 342, 500, 575, 740, 1020, 1466, 1469
NciI CCSGG 1 cut(s) 1632
NcoI CCATGG 1 cut(s) 821
NdeI CATATG 2 cut(s) 1081, 1477
NdeII GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
NlaIV GGNNCC 5 cut(s) 70, 71, 717, 1438, 1809
NmuCI GTSAC 3 cut(s) 697, 1214, 1273
NsiI ATGCAT 1 cut(s) 1682
NspI RCATGY 2 cut(s) 439, 1734
PaeI GCATGC 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 1221
PagI TCATGA 1 cut(s) 598
PaqCI CACCTGC 1 cut(s) 1276
PasI CCCWGGG 1 cut(s) 1343
PciSI GCTCTTC 1 cut(s) 1170
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 2 cut(s) 1487, 1936
PfeI GAWTC 5 cut(s) 281, 620, 1025, 1085, 1889
PkrI GCNGC 9 cut(s) 196, 427, 568, 634, 964, 967, 1462, 1471, 1474
PleI GAGTC 1 cut(s) 1701
PpsI GAGTC 1 cut(s) 1701
PpuMI RGGWCCY 2 cut(s) 69, 715
PshAI GACNNNNGTC 1 cut(s) 1711
PsiI TTATAA 1 cut(s) 1322
Psp5II RGGWCCY 2 cut(s) 69, 715
Psp6I CCWGG 3 cut(s) 132, 1014, 1342
PspGI CCWGG 3 cut(s) 132, 1014, 1342
PspN4I GGNNCC 5 cut(s) 70, 71, 717, 1438, 1809
PspPI GGNCC 6 cut(s) 69, 311, 521, 715, 788, 1295
PspPPI RGGWCCY 2 cut(s) 69, 715
PspXI VCTCGAGB 1 cut(s) 1221
PstI CTGCAG 4 cut(s) 123, 568, 778, 967
PstNI CAGNNNCTG 1 cut(s) 1349
PsuI RGATCY 4 cut(s) 686, 1255, 1742, 1765
RsaI GTAC 4 cut(s) 62, 162, 510, 841
RsaNI GTAC 4 cut(s) 61, 161, 509, 840
SapI GCTCTTC 1 cut(s) 1170
SatI GCNGC 9 cut(s) 195, 426, 567, 633, 963, 966, 1461, 1470, 1473
Sau3AI GATC 9 cut(s) 595, 686, 847, 1129, 1255, 1383, 1595, 1742, 1765
Sau96I GGNCC 6 cut(s) 69, 311, 521, 715, 788, 1295
SbfI CCTGCAGG 1 cut(s) 778
SchI GAGTC 1 cut(s) 1701
ScrFI CCNGG 4 cut(s) 134, 1016, 1344, 1632
SdaI CCTGCAGG 1 cut(s) 778
SduI GDGCHC 1 cut(s) 1096
SfaNI GCATC 5 cut(s) 634, 949, 979, 1045, 1234
SfcI CTRYAG 4 cut(s) 119, 564, 774, 963
Sfr274I CTCGAG 1 cut(s) 1221
SgrAI CRCCGGYG 1 cut(s) 94
SinI GGWCC 5 cut(s) 69, 311, 521, 715, 1295
SlaI CTCGAG 1 cut(s) 1221
SmlI CTYRAG 1 cut(s) 1221
SmoI CTYRAG 1 cut(s) 1221
SpeI ACTAGT 1 cut(s) 701
SphI GCATGC 1 cut(s) 439
Sse8387I CCTGCAGG 1 cut(s) 778
SsiI CCGC 2 cut(s) 1472, 1512
SspMI CTAG 1 cut(s) 702
StyD4I CCNGG 4 cut(s) 132, 1014, 1342, 1630
StyI CCWWGG 1 cut(s) 821
TaaI ACNGT 2 cut(s) 805, 1819
TaiI ACGT 1 cut(s) 669
TaqI TCGA 5 cut(s) 982, 1222, 1382, 1862, 1940
TatI WGTACW 1 cut(s) 60
TauI GCSGC 1 cut(s) 1475
TfiI GAWTC 5 cut(s) 281, 620, 1025, 1085, 1889
TscAI CASTG 6 cut(s) 211, 271, 469, 619, 1219, 1582
TseFI GTSAC 3 cut(s) 697, 1214, 1273
TseI GCWGC 8 cut(s) 194, 425, 566, 632, 962, 965, 1460, 1469
Tsp45I GTSAC 3 cut(s) 697, 1214, 1273
TspDTI ATGAA 6 cut(s) 41, 728, 761, 798, 1565, 1707
TspGWI ACGGA 3 cut(s) 672, 966, 1080
TspRI CASTG 6 cut(s) 211, 271, 469, 619, 1219, 1582
VneI GTGCAC 1 cut(s) 1092
VpaK11BI GGWCC 5 cut(s) 69, 311, 521, 715, 1295
XagI CCTNNNNNAGG 1 cut(s) 567
XapI RAATTY 6 cut(s) 270, 349, 901, 1353, 1569, 1823
XceI RCATGY 2 cut(s) 439, 1734
XhoI CTCGAG 1 cut(s) 1221
XmiI GTMKAC 1 cut(s) 532
XspI CTAG 1 cut(s) 702
Zsp2I ATGCAT 1 cut(s) 1682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.