Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0009468

Physical Location & Seq

Genomic Coordinates Forward (+)

17412990 .. 17419448

Gene Structure 6459 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1153600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2871 bp

ATGGAGTCCAGGATGGATCAGTATGAGCTCATGGAGCAGATCGGCCGCGGAGCTTTCGGCGCTGCCATCCTCGTCCACCACAAGGCGGAGAAGAGGAAATATGTGTTGAAGAAGATCCGCCTTGCACGGCAGACGGAGCGGTGCCGGCGATCCGCCCATCAAGAGATGGCTCTTATTGCCCGAATTCAACACCCGTTTATTGTCGAATTTAAGGAAGCCTGGGTCGAGAAGGGTTGCTATGTTTGCATTGTCACTGGATACTGTGAAGGTGGAGACATGGCCGAGATGATGAAGAAGTCAAATGGGGTGTTTTTTCCCGAGGAGAAACTCTGCAGGTGGTTCACACAGCTATTATTGGCAGTTGAATACCTGCATTCCAATTATGTTCTACATCGTGACCTAAAATGTTCCAACATCTTTCTCACCAAGGATCAGGATATTCGTCTTGGGGATTTTGGTCTTGCTAAAACTTTGAAGGCAGATGACTTGGCTTCTTCGGTGGTTGGGACCCCGAATTACATGTGTCCTGAACTGCTTGCGGACATTCCTTATGGTTTCAAATCTGATATTTGGTCCCTAGGGTGTTGCATATACGAGATGGCTGCTCATCGACCTGCTTTTAAAGCTTTTGATATGGCAGGATTGATAAGCAAGATTAATCGTTCCTCTATAGGTCCTCTGCCTTCCTGTTATTCGCCATCCTTGAAAACACTAATCAAAGGCATGCTGAGAAAGAACCCTGAGCATCGGCCAAGTGCATCTGAAATTTTGAGGCACCCGTATCTGCAACCTTATGTTGATCAGTATCGTCCATCATTTACCACTCGAACAGTCTTCTCCCCAGAGAAACGTGTATCTGTAGGTCATGAATCTCGGAGAAACATGGCAGAAAGCCAAAACAGTAATGGGTCAAGCAGTGATAAAGATGGTTTGCCTTCAATTGAGAAAAACATTCCAGAAATGGACACACACTGTGATAATAAAGCTACTGAGACAGATTTAATTTCTCTTGAGGATGAAGATGGCTTTGATCAGCTCCTGCCTACTGAAGAACATGACCGAAATGTGTGCACTTCAAAAGTGGAAGAACAGTTGATGAAACCACCTCATTCTGAATCTAATGTTGAATCGAGGCAACCAAAGACCATTAGGAATATAATGCTGGCTTTGAGAGAAGGAAAAACTAGGGAACATAGCTCACCAATGAGAAGTAATCGGTCTAAGGTTTGTCTGCCGCAAAGAACTAACGTAGAAACAGTGTCCAGAATACCCAAGCTGAGTGATGTCTCTCCTGTTATCAAGCCCAGTCCCGATGCACCAACTGTTGCATGTTCAAAGGCAACCCTCGATCCTGAAAAACGGACACAGGGATCCCATCCTTTAAAGCATCAGTTGCCTGTAATTGAATACTCACCGAAATCTAAACCCAGACATGATGGTACTCCCCCTGTTCCTATAAAACAAATTGAAGATGGACTTCCAAAGCCTAGGCAAAGAACCCCTCCTACACTGGTTAGACGTCCTTCATTTCCTGCGCGGATGAAGCAAGTGGGGGTTGATGTTCCGAATGCTGTGAACTATGTTGCAAGGATAAGTCCAAGGGAGATAAACCAGGAGCCTGAAAAGAATCCCCCTCAAGTGCCCAATGGTTGCCTAACAAACTCTCCTAGAGAAGTCCAGCAAGAGCCTCAAAGATATCAAGAAACTAGTTCAAAAGGATTGCAAACGGACAGCAGCAATTCTGTCTCCTCTTCTGCATCAATTCAAGGATTTGAGATTTGTGATGATGCAACAACTGCTTTTATTGACATGAGAGTGCAGGTGCTTCCTGATTATGGGAGCGTCACCCACACTGAGAGTACAGAGTCTCAACCGAGCTGTTCACCCGCTTCTCATTTTCATTCAGTGATATCTGAAACTGCTGAAAAAGATGGGAATGATAATAAGTCTGAATGCTTAATTGAAACATCTGAGGCTATTCTAGATCTTCAGAATACAGCTGCTGGTGACCAACAAGTGAGTTTAATTGCCACTCTCGATCTCCCTGACCCAGTTTCTGAAGAAAGATTTATTTGTAATGATCATACCCCTGTAAGTATGCCCAGTTGTATGCCTTATACAGTGGATCAAGCAAAACATACATCTACATCTAGTGGTCACGACAAGTTCACTGTAAGGGAACATCAATCCCCAGTAGCGGACACTACCCTTTCTGTTACCCCTCCCATTTCATCAAGCCAAAATTTATTTCAACCAGATCAAGGAACGGGTTTGCAAAATCAGTTAATGGAAAAGTCGACTGCGGCTAATCTTGCCCCTGCTTTTGATGACATCATACATGTGATTAGGCATAGTAGTTTTCGTGTTGGGAGTGATCAACCAGTAATGGAATCCGTGGAGATGGGGGTTCAGAATGTGGATGTTGGTAAGCTGATAAATGTGGTAAGGGATGAATTGGAGATGCGAAATGCGACTGGCCTTGTGGCTCAGAAATCATCAAACTGCGCTGAGACAGTGAGCCCGGAATCAAATAACTCTGATCTCTCTAATGTCAAAGAACCCGAGATAAGAAACCCGATTCCTTCAGCAAAAATATCCGAATCCTCTGAGCCTACAAAGTCCAGTCCTCCGGCGGAAGAGGAAATACCAGAGAAGGAAATCATGGATGTCAAGTCTTTTAGGCAGAGGGCAGAGGCACTAGAAGGACTACTAGAATTGTCTGCAGATTTGCTGCAGAATAACAGATTAGAGGAACTCTCAGTTGTTTTGAGGCCTTTTGGGAAAGATAAAGTCTCCCCAAGAGAGACGGCTATCTGGTTAGCAAAGAGTCTGAAAGGAATGAGGATTGAGGATGGCGGGCGGAGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0008152 GO:0008544 GO:0009888 GO:0009913 GO:0009987 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0030154 GO:0030855 GO:0030863 GO:0030865 GO:0030981 GO:0031122 GO:0032502 GO:0036211 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0043622 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048856 GO:0048869 GO:0055028 GO:0060429 GO:0071704 GO:0071840 GO:0071944 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

957

Amino Acids

106.41

Weight (kDa)

5.92

Isoelectric Point (pI)

57.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	8 - 262	3.3e-66	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	9 - 258	5.3e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009468)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44200 AT3G44200
fragaria_vesca	FvH4_3g26671 FvH4_3g26671 FvH4_3g26671
malus_domestica	MD00G1129100.v1.1 MD03G1153600.v1.1
prunus_persica	Prupe.6G142700_v2.0.a1 Prupe.6G142700_v2.0.a1 Prupe.6G142700_v2.0.a1
pyrus_communis	pycom03g10730 pycom11g14600
rosa_chinensis	RchiOBHm_Chr5g0048101
rosa_laevigata	RLG00000034496
rosa_multiflora	Rmu_sc0001408.1_g000034
rosa_roxburghii	Rroxscaffold_1G00033820
rosa_rugosa	Rorug05G0236600
rosa_samantha	Rh5AG315800 Rh5BG326000 Rh5CG352500 Rh5DG337200
rosa_wichuraiana	Rw5G029600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	324, 1810
AatII	GACGTC	1 cut(s)	1522
Acc36I	ACCTGC	4 cut(s)	324, 378, 622, 1810
AccB1I	GGYRCC	2 cut(s)	141, 774
AccBSI	CCGCTC	1 cut(s)	139
AccI	GTMKAC	1 cut(s)	2297
AccII	CGCG	2 cut(s)	48, 1537
AclWI	GGATC	8 cut(s)	24, 109, 144, 438, 1343, 1365, 1378, 2133
AcoI	YGGCCR	3 cut(s)	43, 279, 749
AcsI	RAATTY	4 cut(s)	183, 206, 765, 2242
AcuI	CTGAAG	4 cut(s)	1068, 1973, 2079, 2568
AcyI	GRCGYC	1 cut(s)	1519
AdeI	CACNNNGTG	1 cut(s)	974
AfaI	GTAC	2 cut(s)	1441, 1861
AfiI	CCNNNNNNNGG	5 cut(s)	82, 85, 1835, 2197, 2261
AflIII	ACRYGT	3 cut(s)	519, 850, 2338
AhlI	ACTAGT	1 cut(s)	1706
AjnI	CCWGG	3 cut(s)	8, 218, 1611
AjuI	GAANNNNNNNTTGG	2 cut(s)	2232, 2264
Alw21I	GWGCWC	2 cut(s)	30, 1073
Alw26I	GTCTC	8 cut(s)	267, 986, 1291, 1750, 1872, 2504, 2797, 2798
Alw44I	GTGCAC	1 cut(s)	1069
AlwI	GGATC	8 cut(s)	24, 109, 144, 438, 1343, 1365, 1378, 2133
AlwNI	CAGNNNCTG	3 cut(s)	1039, 2003, 2057
Ama87I	CYCGRG	2 cut(s)	317, 2561
AoxI	GGCC	5 cut(s)	43, 279, 749, 2476, 2771
ApaLI	GTGCAC	1 cut(s)	1069
ApeKI	GCWGC	5 cut(s)	62, 602, 1734, 2000, 2731
ApoI	RAATTY	4 cut(s)	183, 206, 765, 2242
AseI	ATTAAT	1 cut(s)	657
AspA2I	CCTAGG	2 cut(s)	577, 1487
AspLEI	GCGC	3 cut(s)	62, 1537, 2507
AspS9I	GGNCC	3 cut(s)	507, 573, 674
AsuC2I	CCSGG	1 cut(s)	2522
AsuHPI	GGTGA	6 cut(s)	415, 1191, 1404, 1837, 1875, 2018
AvaI	CYCGRG	2 cut(s)	317, 2561
AvaII	GGWCC	3 cut(s)	507, 573, 674
AvrII	CCTAGG	2 cut(s)	577, 1487
BaeGI	GKGCMC	2 cut(s)	1073, 1644
BamHI	GGATCC	1 cut(s)	1370
BanI	GGYRCC	2 cut(s)	141, 774
BanII	GRGCYC	2 cut(s)	30, 2522
BarI	GAAGNNNNNNTAC	2 cut(s)	2628, 2660
BbsI	GAAGAC	1 cut(s)	826
Bbv12I	GWGCWC	2 cut(s)	30, 1073
BbvI	GCAGC	5 cut(s)	49, 589, 1746, 1987, 2718
BceAI	ACGGC	2 cut(s)	143, 2823
BcgI	CGANNNNNNTGC	4 cut(s)	584, 618, 1050, 1084
BciT130I	CCWGG	3 cut(s)	10, 220, 1613
BciVI	GTATCC	1 cut(s)	251
BclI	TGATCA	4 cut(s)	799, 1030, 2080, 2374
BcnI	CCSGG	1 cut(s)	2522
BcoDI	GTCTC	8 cut(s)	267, 986, 1291, 1750, 1872, 2504, 2797, 2798
BcuI	ACTAGT	1 cut(s)	1706
BfaI	CTAG	9 cut(s)	578, 1185, 1488, 1668, 1707, 1982, 2151, 2699, 2711
BfmI	CTRYAG	5 cut(s)	331, 669, 858, 2721, 2732
BfoI	RGCGCY	1 cut(s)	63
BfuAI	ACCTGC	4 cut(s)	324, 378, 622, 1810
BfuI	GTATCC	1 cut(s)	251
BglII	AGATCT	1 cut(s)	1984
BisI	GCNGC	8 cut(s)	46, 63, 603, 1235, 1735, 2001, 2304, 2732
BlnI	CCTAGG	2 cut(s)	577, 1487
BlsI	GCNGC	8 cut(s)	47, 64, 604, 1236, 1736, 2002, 2305, 2733
Bme1390I	CCNGG	4 cut(s)	10, 220, 1613, 2522
Bme18I	GGWCC	3 cut(s)	507, 573, 674
BmeT110I	CYCGRG	2 cut(s)	317, 2561
BmgT120I	GGNCC	3 cut(s)	507, 573, 674
BmiI	GGNNCC	7 cut(s)	143, 508, 509, 575, 776, 1372, 1617
BmrFI	CCNGG	4 cut(s)	10, 220, 1613, 2522
BmrI	ACTGGG	4 cut(s)	1299, 2045, 2097, 2186
BmsI	GCATC	7 cut(s)	754, 767, 1303, 1396, 1766, 1777, 2451
BmuI	ACTGGG	4 cut(s)	1299, 2045, 2097, 2186
BpiI	GAAGAC	1 cut(s)	826
BplI	GAGNNNNNCTC	2 cut(s)	2741, 2773
Bpu10I	CCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	2 cut(s)	1031, 1620
BpuMI	CCSGG	1 cut(s)	2522
BsaBI	GATNNNNATC	1 cut(s)	804
BsaHI	GRCGYC	1 cut(s)	1519
BsaJI	CCNNGG	8 cut(s)	46, 219, 318, 426, 577, 1487, 1598, 2394
BsaXI	ACNNNNNCTCC	2 cut(s)	1648, 1678
Bsc4I	CCNNNNNNNGG	5 cut(s)	82, 85, 1835, 2197, 2261
Bse118I	RCCGGY	1 cut(s)	144
Bse1I	ACTGG	9 cut(s)	259, 1305, 1515, 2051, 2103, 2192, 2381, 2479, 2622
Bse8I	GATNNNNATC	1 cut(s)	804
BseBI	CCWGG	3 cut(s)	10, 220, 1613
BseDI	CCNNGG	8 cut(s)	46, 219, 318, 426, 577, 1487, 1598, 2394
BseJI	GATNNNNATC	1 cut(s)	804
BseLI	CCNNNNNNNGG	5 cut(s)	82, 85, 1835, 2197, 2261
BseNI	ACTGG	9 cut(s)	259, 1305, 1515, 2051, 2103, 2192, 2381, 2479, 2622
BseRI	GAGGAG	2 cut(s)	335, 1738
BseSI	GKGCMC	2 cut(s)	1073, 1644
BseX3I	CGGCCG	1 cut(s)	43
BseXI	GCAGC	5 cut(s)	49, 589, 1746, 1987, 2718
BsgI	GTGCAG	1 cut(s)	1838
Bsh1236I	CGCG	2 cut(s)	48, 1537
Bsh1285I	CGRYCG	1 cut(s)	46
BshFI	GGCC	5 cut(s)	45, 281, 751, 2478, 2773
BshNI	GGYRCC	2 cut(s)	141, 774
BsiEI	CGRYCG	1 cut(s)	46
BsiHKAI	GWGCWC	2 cut(s)	30, 1073
BsiHKCI	CYCGRG	2 cut(s)	317, 2561
BsiSI	CCGG	3 cut(s)	145, 2522, 2630
BslFI	GGGAC	3 cut(s)	520, 559, 1293
BslI	CCNNNNNNNGG	5 cut(s)	82, 85, 1835, 2197, 2261
BsmAI	GTCTC	8 cut(s)	267, 986, 1291, 1750, 1872, 2504, 2797, 2798
BsmBI	CGTCTC	1 cut(s)	2798
BsmFI	GGGAC	3 cut(s)	520, 559, 1293
BsmI	GAATGC	3 cut(s)	373, 1573, 1958
BsnI	GGCC	5 cut(s)	45, 281, 751, 2478, 2773
BsoBI	CYCGRG	2 cut(s)	317, 2561
Bsp1286I	GDGCHC	4 cut(s)	30, 1073, 1644, 2522
BspANI	GGCC	5 cut(s)	45, 281, 751, 2478, 2773
BspFNI	CGCG	2 cut(s)	48, 1537
BspHI	TCATGA	2 cut(s)	865, 2867
BspLI	GGNNCC	7 cut(s)	143, 508, 509, 575, 776, 1372, 1617
BspMAI	CTGCAG	3 cut(s)	335, 2725, 2736
BspMI	ACCTGC	4 cut(s)	324, 378, 622, 1810
BspPI	GGATC	8 cut(s)	24, 109, 144, 438, 1343, 1365, 1378, 2133
BspT107I	GGYRCC	2 cut(s)	141, 774
BsrBI	CCGCTC	1 cut(s)	139
BsrFI	RCCGGY	1 cut(s)	144
BsrI	ACTGG	9 cut(s)	259, 1305, 1515, 2051, 2103, 2192, 2381, 2479, 2622
BssAI	RCCGGY	1 cut(s)	144
BssECI	CCNNGG	8 cut(s)	46, 219, 318, 426, 577, 1487, 1598, 2394
BssNI	GRCGYC	1 cut(s)	1519
BssT1I	CCWWGG	4 cut(s)	426, 577, 1487, 1598
Bst2UI	CCWGG	3 cut(s)	10, 220, 1613
Bst6I	CTCTTC	3 cut(s)	86, 1756, 2631
BstACI	GRCGYC	1 cut(s)	1519
BstAPI	GCANNNNNTGC	2 cut(s)	1393, 1796
BstC8I	GCNNGC	5 cut(s)	146, 537, 725, 1164, 2858
BstDSI	CCRYGG	2 cut(s)	46, 2394
BstEII	GGTNACC	1 cut(s)	2006
BstFNI	CGCG	2 cut(s)	48, 1537
BstH2I	RGCGCY	1 cut(s)	63
BstHHI	GCGC	3 cut(s)	62, 1537, 2507
BstMAI	GTCTC	8 cut(s)	267, 986, 1291, 1750, 1872, 2504, 2797, 2798
BstMCI	CGRYCG	1 cut(s)	46
BstNI	CCWGG	3 cut(s)	10, 220, 1613
BstNSI	RCATGY	4 cut(s)	523, 727, 1332, 2342
BstPI	GGTNACC	1 cut(s)	2006
BstSCI	CCNGG	4 cut(s)	8, 218, 1611, 2520
BstSFI	CTRYAG	5 cut(s)	331, 669, 858, 2721, 2732
BstSLI	GKGCMC	2 cut(s)	1073, 1644
BstUI	CGCG	2 cut(s)	48, 1537
BstV1I	GCAGC	5 cut(s)	49, 589, 1746, 1987, 2718
BstV2I	GAAGAC	1 cut(s)	826
BstX2I	RGATCY	3 cut(s)	114, 1370, 1984
BstYI	RGATCY	3 cut(s)	114, 1370, 1984
BstZI	CGGCCG	1 cut(s)	43
BsuI	GTATCC	1 cut(s)	251
BsuRI	GGCC	5 cut(s)	45, 281, 751, 2478, 2773
BtgI	CCRYGG	2 cut(s)	46, 2394
BtsI	GCAGTG	1 cut(s)	922
BveI	ACCTGC	4 cut(s)	324, 378, 622, 1810
Cac8I	GCNNGC	5 cut(s)	146, 537, 725, 1164, 2858
CaiI	CAGNNNCTG	3 cut(s)	1039, 2003, 2057
CciI	TCATGA	2 cut(s)	865, 2867
CfoI	GCGC	3 cut(s)	62, 1537, 2507
Cfr10I	RCCGGY	1 cut(s)	144
Cfr13I	GGNCC	3 cut(s)	507, 573, 674
Cfr42I	CCGCGG	1 cut(s)	49
CseI	GACGC	1 cut(s)	1831
Csp6I	GTAC	2 cut(s)	1440, 1860
CviQI	GTAC	2 cut(s)	1440, 1860
DraI	TTTAAA	2 cut(s)	622, 1383
DraIII	CACNNNGTG	1 cut(s)	974
EaeI	YGGCCR	3 cut(s)	43, 279, 749
EagI	CGGCCG	1 cut(s)	43
Eam1104I	CTCTTC	3 cut(s)	86, 1756, 2631
EarI	CTCTTC	3 cut(s)	86, 1756, 2631
EciI	GGCGGA	4 cut(s)	101, 107, 142, 2648
Ecl136II	GAGCTC	1 cut(s)	28
EclXI	CGGCCG	1 cut(s)	43
Eco130I	CCWWGG	4 cut(s)	426, 577, 1487, 1598
Eco147I	AGGCCT	1 cut(s)	2773
Eco24I	GRGCYC	2 cut(s)	30, 2522
Eco32I	GATATC	2 cut(s)	1697, 1911
Eco47I	GGWCC	3 cut(s)	507, 573, 674
Eco52I	CGGCCG	1 cut(s)	43
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	4 cut(s)	1068, 1973, 2079, 2568
Eco88I	CYCGRG	2 cut(s)	317, 2561
Eco91I	GGTNACC	1 cut(s)	2006
EcoICRI	GAGCTC	1 cut(s)	28
EcoO109I	RGGNCCY	2 cut(s)	507, 674
EcoO65I	GGTNACC	1 cut(s)	2006
EcoRI	GAATTC	1 cut(s)	183
EcoRII	CCWGG	3 cut(s)	8, 218, 1611
EcoRV	GATATC	2 cut(s)	1697, 1911
EcoT14I	CCWWGG	4 cut(s)	426, 577, 1487, 1598
EcoT38I	GRGCYC	2 cut(s)	30, 2522
ErhI	CCWWGG	4 cut(s)	426, 577, 1487, 1598
Esp3I	CGTCTC	1 cut(s)	2798
FalI	AAGNNNNNCTT	2 cut(s)	1461, 1493
FaqI	GGGAC	3 cut(s)	520, 559, 1293
FauI	CCCGC	2 cut(s)	1894, 2849
FbaI	TGATCA	4 cut(s)	799, 1030, 2080, 2374
FblI	GTMKAC	1 cut(s)	2297
Fnu4HI	GCNGC	8 cut(s)	46, 63, 603, 1235, 1735, 2001, 2304, 2732
FriOI	GRGCYC	2 cut(s)	30, 2522
Fsp4HI	GCNGC	8 cut(s)	46, 63, 603, 1235, 1735, 2001, 2304, 2732
FspBI	CTAG	9 cut(s)	578, 1185, 1488, 1668, 1707, 1982, 2151, 2699, 2711
GlaI	GCGC	3 cut(s)	61, 1536, 2506
GluI	GCNGC	8 cut(s)	46, 63, 603, 1235, 1735, 2001, 2304, 2732
HaeII	RGCGCY	1 cut(s)	63
HaeIII	GGCC	5 cut(s)	45, 281, 751, 2478, 2773
HapII	CCGG	3 cut(s)	145, 2522, 2630
HgaI	GACGC	1 cut(s)	1831
HhaI	GCGC	3 cut(s)	62, 1537, 2507
Hin1I	GRCGYC	1 cut(s)	1519
Hin6I	GCGC	3 cut(s)	60, 1535, 2505
HinP1I	GCGC	3 cut(s)	60, 1535, 2505
HincII	GTYRAC	1 cut(s)	2298
HindII	GTYRAC	1 cut(s)	2298
HindIII	AAGCTT	1 cut(s)	624
HpaII	CCGG	3 cut(s)	145, 2522, 2630
HphI	GGTGA	6 cut(s)	415, 1191, 1404, 1837, 1875, 2018
Hpy166II	GTNNAC	7 cut(s)	76, 342, 1071, 1576, 1883, 2169, 2298
Hpy8I	GTNNAC	7 cut(s)	76, 342, 1071, 1576, 1883, 2169, 2298
HpyCH4IV	ACGT	3 cut(s)	850, 1248, 1519
HpySE526I	ACGT	3 cut(s)	850, 1248, 1519
Hsp92I	GRCGYC	1 cut(s)	1519
HspAI	GCGC	3 cut(s)	60, 1535, 2505
KflI	GGGWCCC	1 cut(s)	507
KroI	GCCGGC	1 cut(s)	144
KroNI	GCCGGC	1 cut(s)	146
Ksp22I	TGATCA	4 cut(s)	799, 1030, 2080, 2374
KspI	CCGCGG	1 cut(s)	49
LmnI	GCTCC	6 cut(s)	34, 50, 136, 1041, 1615, 1839
Lsp1109I	GCAGC	5 cut(s)	49, 589, 1746, 1987, 2718
LweI	GCATC	7 cut(s)	754, 767, 1303, 1396, 1766, 1777, 2451
MaeI	CTAG	9 cut(s)	578, 1185, 1488, 1668, 1707, 1982, 2151, 2699, 2711
MaeII	ACGT	3 cut(s)	850, 1248, 1519
MaeIII	GTNAC	6 cut(s)	250, 395, 1843, 2006, 2156, 2215
MbiI	CCGCTC	1 cut(s)	139
MfeI	CAATTG	1 cut(s)	939
MflI	RGATCY	3 cut(s)	114, 1370, 1984
MhlI	GDGCHC	4 cut(s)	30, 1073, 1644, 2522
MlyI	GAGTC	3 cut(s)	14, 1874, 2836
MmeI	TCCRAC	1 cut(s)	435
MroNI	GCCGGC	1 cut(s)	144
MseI	TTAA	8 cut(s)	210, 621, 657, 1001, 1382, 1958, 2024, 2285
MslI	CAYNNNNRTG	2 cut(s)	1814, 2339
MspA1I	CMGCKG	2 cut(s)	48, 2000
MspI	CCGG	3 cut(s)	145, 2522, 2630
MspR9I	CCNGG	4 cut(s)	10, 220, 1613, 2522
MunI	CAATTG	1 cut(s)	939
Mva1269I	GAATGC	3 cut(s)	373, 1573, 1958
MvaI	CCWGG	3 cut(s)	10, 220, 1613
MvnI	CGCG	2 cut(s)	48, 1537
NaeI	GCCGGC	1 cut(s)	146
NciI	CCSGG	1 cut(s)	2522
NgoMIV	GCCGGC	1 cut(s)	144
NlaIV	GGNNCC	7 cut(s)	143, 508, 509, 575, 776, 1372, 1617
NmeAIII	GCCGAG	1 cut(s)	307
NmuCI	GTSAC	5 cut(s)	250, 395, 1843, 2006, 2156
NspI	RCATGY	4 cut(s)	523, 727, 1332, 2342
PaeI	GCATGC	1 cut(s)	727
PagI	TCATGA	2 cut(s)	865, 2867
PaqCI	CACCTGC	2 cut(s)	324, 1810
PceI	AGGCCT	1 cut(s)	2773
PciI	ACATGT	2 cut(s)	519, 2338
PctI	GAATGC	3 cut(s)	373, 1573, 1958
PdiI	GCCGGC	1 cut(s)	146
PfeI	GAWTC	8 cut(s)	869, 1115, 1127, 1627, 2390, 2525, 2578, 2600
PfoI	TCCNGGA	1 cut(s)	8
PkrI	GCNGC	8 cut(s)	47, 64, 604, 1236, 1736, 2002, 2305, 2733
PleI	GAGTC	3 cut(s)	13, 1873, 2835
PpsI	GAGTC	3 cut(s)	13, 1873, 2835
PpuMI	RGGWCCY	2 cut(s)	507, 674
PscI	ACATGT	2 cut(s)	519, 2338
PshBI	ATTAAT	1 cut(s)	657
Psp124BI	GAGCTC	1 cut(s)	30
Psp5II	RGGWCCY	2 cut(s)	507, 674
Psp6I	CCWGG	3 cut(s)	8, 218, 1611
PspEI	GGTNACC	1 cut(s)	2006
PspGI	CCWGG	3 cut(s)	8, 218, 1611
PspN4I	GGNNCC	7 cut(s)	143, 508, 509, 575, 776, 1372, 1617
PspPI	GGNCC	3 cut(s)	507, 573, 674
PspPPI	RGGWCCY	2 cut(s)	507, 674
PsrI	GAACNNNNNNTAC	2 cut(s)	1489, 1521
PstI	CTGCAG	3 cut(s)	335, 2725, 2736
PstNI	CAGNNNCTG	3 cut(s)	1039, 2003, 2057
PsuI	RGATCY	3 cut(s)	114, 1370, 1984
PvuII	CAGCTG	1 cut(s)	2000
RsaI	GTAC	2 cut(s)	1441, 1861
RsaNI	GTAC	2 cut(s)	1440, 1860
RseI	CAYNNNNRTG	2 cut(s)	1814, 2339
SacI	GAGCTC	1 cut(s)	30
SacII	CCGCGG	1 cut(s)	49
SalI	GTCGAC	1 cut(s)	2296
SaqAI	TTAA	8 cut(s)	210, 621, 657, 1001, 1382, 1958, 2024, 2285
SatI	GCNGC	8 cut(s)	46, 63, 603, 1235, 1735, 2001, 2304, 2732
Sau96I	GGNCC	3 cut(s)	507, 573, 674
SchI	GAGTC	3 cut(s)	14, 1874, 2836
ScrFI	CCNGG	4 cut(s)	10, 220, 1613, 2522
SduI	GDGCHC	4 cut(s)	30, 1073, 1644, 2522
SfaNI	GCATC	7 cut(s)	754, 767, 1303, 1396, 1766, 1777, 2451
SfcI	CTRYAG	5 cut(s)	331, 669, 858, 2721, 2732
Sfr303I	CCGCGG	1 cut(s)	49
SgrBI	CCGCGG	1 cut(s)	49
SinI	GGWCC	3 cut(s)	507, 573, 674
SmiMI	CAYNNNNRTG	2 cut(s)	1814, 2339
SmlI	CTYRAG	2 cut(s)	1010, 1635
SmoI	CTYRAG	2 cut(s)	1010, 1635
SpeI	ACTAGT	1 cut(s)	1706
SphI	GCATGC	1 cut(s)	727
SseBI	AGGCCT	1 cut(s)	2773
SspMI	CTAG	9 cut(s)	578, 1185, 1488, 1668, 1707, 1982, 2151, 2699, 2711
SstI	GAGCTC	1 cut(s)	30
StuI	AGGCCT	1 cut(s)	2773
StyD4I	CCNGG	4 cut(s)	8, 218, 1611, 2520
StyI	CCWWGG	4 cut(s)	426, 577, 1487, 1598
TaiI	ACGT	3 cut(s)	853, 1251, 1522
TaqI	TCGA	8 cut(s)	204, 225, 610, 826, 1130, 1347, 2037, 2297
TaqII	GACCGA	2 cut(s)	1074, 1206
TatI	WGTACW	1 cut(s)	1859
TauI	GCSGC	3 cut(s)	48, 1237, 2306
TfiI	GAWTC	8 cut(s)	869, 1115, 1127, 1627, 2390, 2525, 2578, 2600
Tru1I	TTAA	8 cut(s)	210, 621, 657, 1001, 1382, 1958, 2024, 2285
Tru9I	TTAA	8 cut(s)	210, 621, 657, 1001, 1382, 1958, 2024, 2285
TseFI	GTSAC	5 cut(s)	250, 395, 1843, 2006, 2156
TseI	GCWGC	5 cut(s)	62, 602, 1734, 2000, 2731
Tsp45I	GTSAC	5 cut(s)	250, 395, 1843, 2006, 2156
TspGWI	ACGGA	4 cut(s)	149, 1375, 1742, 2383
VneI	GTGCAC	1 cut(s)	1069
VpaK11BI	GGWCC	3 cut(s)	507, 573, 674
VspI	ATTAAT	1 cut(s)	657
XapI	RAATTY	4 cut(s)	183, 206, 765, 2242
XbaI	TCTAGA	1 cut(s)	1981
XceI	RCATGY	4 cut(s)	523, 727, 1332, 2342
XcmI	CCANNNNNNNNNTGG	1 cut(s)	902
XmaJI	CCTAGG	2 cut(s)	577, 1487
XmiI	GTMKAC	1 cut(s)	2297
XspI	CTAG	9 cut(s)	578, 1185, 1488, 1668, 1707, 1982, 2151, 2699, 2711
ZraI	GACGTC	1 cut(s)	1520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.