MD03G1218600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028477
Physical Location & Seq
Forward (+)
30331251 .. 30332402
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1218600.v1.1.491

Sequence Viewer

Length: 369 bp
ATGGATTTTCCGGCCAATTTACAGCCAAGCTTGCCGGAATTGGACCTCGACCCAAGAGGTAAAGATGATCCTCTCCTCGTGGGCTGTCTTCCAGTATTGGGGACGAGGAAGGTGAATCAAGGTTGTTTGAGACCTGGGCCGAGAAGAATGGAAGTTTTTGTAGGTCTTAGTTGCTGGAAGAGACGAGGAAGAGCGTGCCGAAGAGTGGAAAGAGCTCGAAGGATCCGGATGAGCAGAATGGGGAGGAGGAAGCAGCGGAAGAGATTGGGAAGAAGGCGGCCAAGTGGGCATTGCCCGGATTGTAGCCTCCTAAGGCACCGGAAACACAGGTCTCGTCTTCTTCGTTTTTCCCGCCAAACTTGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

14.34

Weight (kDa)

11.75

Isoelectric Point (pI)

94.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028477)

Species Orthologous Gene IDs
malus_domestica MD03G1218600.v1.1
pyrus_communis pycom03g16790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 315
AccIII TCCGGA 1 cut(s) 225
AciI CCGC 3 cut(s) 256, 277, 352
AclWI GGATC 3 cut(s) 62, 217, 230
AcoI YGGCCR 2 cut(s) 12, 278
AfiI CCNNNNNNNGG 2 cut(s) 98, 205
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 2 cut(s) 30, 215
AluI AGCT 2 cut(s) 30, 215
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 3 cut(s) 124, 175, 336
AlwI GGATC 3 cut(s) 62, 217, 230
Aor13HI TCCGGA 1 cut(s) 225
AoxI GGCC 3 cut(s) 12, 137, 278
ApeKI GCWGC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 43, 137
AsuC2I CCSGG 1 cut(s) 296
AsuHPI GGTGA 1 cut(s) 124
AvaII GGWCC 1 cut(s) 43
AxyI CCTNAGG 1 cut(s) 311
BamHI GGATCC 1 cut(s) 222
BanI GGYRCC 1 cut(s) 315
BanII GRGCYC 1 cut(s) 217
BauI CACGAG 1 cut(s) 77
BbsI GAAGAC 2 cut(s) 80, 329
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 265
BciT130I CCWGG 1 cut(s) 135
BcnI CCSGG 1 cut(s) 296
BcoDI GTCTC 3 cut(s) 124, 175, 336
BglI GCCNNNNNGGC 1 cut(s) 286
BisI GCNGC 2 cut(s) 254, 278
BlsI GCNGC 2 cut(s) 255, 279
Bme1390I CCNGG 2 cut(s) 135, 296
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 43, 137
BmiI GGNNCC 2 cut(s) 224, 317
BmrFI CCNGG 2 cut(s) 135, 296
BpiI GAAGAC 2 cut(s) 80, 329
BpuMI CCSGG 1 cut(s) 296
BsaI GGTCTC 2 cut(s) 124, 336
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 2 cut(s) 225, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 205
Bse1I ACTGG 1 cut(s) 92
Bse21I CCTNAGG 1 cut(s) 311
Bse3DI GCAATG 1 cut(s) 289
BseAI TCCGGA 1 cut(s) 225
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 98, 205
BseMI GCAATG 1 cut(s) 289
BseNI ACTGG 1 cut(s) 92
BseRI GAGGAG 2 cut(s) 65, 259
BseXI GCAGC 1 cut(s) 265
BshFI GGCC 3 cut(s) 14, 139, 280
BshNI GGYRCC 1 cut(s) 315
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 5 cut(s) 11, 35, 226, 296, 319
BslFI GGGAC 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 98, 205
BsmAI GTCTC 3 cut(s) 124, 175, 336
BsmBI CGTCTC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 115
BsnI GGCC 3 cut(s) 14, 139, 280
Bso31I GGTCTC 2 cut(s) 124, 336
Bsp1286I GDGCHC 1 cut(s) 217
Bsp13I TCCGGA 1 cut(s) 225
Bsp143I GATC 2 cut(s) 67, 222
BspACI CCGC 3 cut(s) 256, 277, 352
BspANI GGCC 3 cut(s) 14, 139, 280
BspEI TCCGGA 1 cut(s) 225
BspLI GGNNCC 2 cut(s) 224, 317
BspPI GGATC 3 cut(s) 62, 217, 230
BspQI GCTCTTC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 315
BspTNI GGTCTC 2 cut(s) 124, 336
BsrDI GCAATG 1 cut(s) 289
BsrI ACTGG 1 cut(s) 92
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 2 cut(s) 67, 222
BssSI CACGAG 1 cut(s) 77
Bst2BI CACGAG 1 cut(s) 77
Bst2UI CCWGG 1 cut(s) 135
Bst6I CTCTTC 4 cut(s) 173, 184, 196, 254
BstC8I GCNNGC 2 cut(s) 32, 196
BstDEI CTNAG 2 cut(s) 167, 311
BstF5I GGATG 1 cut(s) 234
BstKTI GATC 2 cut(s) 70, 225
BstMAI GTCTC 3 cut(s) 124, 175, 336
BstMBI GATC 2 cut(s) 67, 222
BstMWI GCNNNNNNNGC 2 cut(s) 31, 286
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 133, 294
BstV1I GCAGC 1 cut(s) 265
BstV2I GAAGAC 2 cut(s) 80, 329
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
Bsu36I CCTNAGG 1 cut(s) 311
BsuRI GGCC 3 cut(s) 14, 139, 280
BtsCI GGATG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 32, 196
Cfr13I GGNCC 2 cut(s) 43, 137
CviJI RGCY 8 cut(s) 14, 25, 30, 84, 139, 215, 280, 306
CviKI_1 RGCY 8 cut(s) 14, 25, 30, 84, 139, 215, 280, 306
DdeI CTNAG 2 cut(s) 167, 311
DpnI GATC 2 cut(s) 69, 224
DpnII GATC 2 cut(s) 67, 222
EaeI YGGCCR 2 cut(s) 12, 278
Eam1104I CTCTTC 4 cut(s) 173, 184, 196, 254
EarI CTCTTC 4 cut(s) 173, 184, 196, 254
Ecl136II GAGCTC 1 cut(s) 215
Eco24I GRGCYC 1 cut(s) 217
Eco31I GGTCTC 2 cut(s) 124, 336
Eco47I GGWCC 1 cut(s) 43
Eco53kI GAGCTC 1 cut(s) 215
Eco81I CCTNAGG 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 215
EcoRII CCWGG 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 217
Esp3I CGTCTC 1 cut(s) 175
FaqI GGGAC 1 cut(s) 115
FauI CCCGC 1 cut(s) 359
Fnu4HI GCNGC 2 cut(s) 254, 278
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 254, 278
GluI GCNGC 2 cut(s) 254, 278
HaeIII GGCC 3 cut(s) 14, 139, 280
HapII CCGG 5 cut(s) 11, 35, 226, 296, 319
HindIII AAGCTT 1 cut(s) 28
HinfI GANTC 1 cut(s) 115
HpaII CCGG 5 cut(s) 11, 35, 226, 296, 319
HphI GGTGA 1 cut(s) 124
Hpy188III TCNNGA 1 cut(s) 226
HpyAV CCTTC 3 cut(s) 103, 213, 267
HpyF10VI GCNNNNNNNGC 2 cut(s) 31, 286
HpyF3I CTNAG 2 cut(s) 167, 311
Kpn2I TCCGGA 1 cut(s) 225
Kzo9I GATC 2 cut(s) 67, 222
LguI GCTCTTC 1 cut(s) 184
Lsp1109I GCAGC 1 cut(s) 265
MalI GATC 2 cut(s) 69, 224
MboI GATC 2 cut(s) 67, 222
MboII GAAGA 9 cut(s) 80, 156, 190, 201, 213, 271, 282, 329, 332
MflI RGATCY 1 cut(s) 222
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 16, 38
MnlI CCTC 9 cut(s) 50, 56, 81, 86, 99, 179, 237, 240, 317
MroI TCCGGA 1 cut(s) 225
MspA1I CMGCKG 1 cut(s) 256
MspI CCGG 5 cut(s) 11, 35, 226, 296, 319
MspR9I CCNGG 2 cut(s) 135, 296
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 2 cut(s) 31, 286
NciI CCSGG 1 cut(s) 296
NdeII GATC 2 cut(s) 67, 222
NlaIV GGNNCC 2 cut(s) 224, 317
NmeAIII GCCGAG 1 cut(s) 165
PciSI GCTCTTC 1 cut(s) 184
PcsI WCGNNNNNNNCGW 1 cut(s) 340
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 2 cut(s) 255, 279
Psp124BI GAGCTC 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 224, 317
PspPI GGNCC 2 cut(s) 43, 137
PsuI RGATCY 1 cut(s) 222
SacI GAGCTC 1 cut(s) 217
SapI GCTCTTC 1 cut(s) 184
SatI GCNGC 2 cut(s) 254, 278
Sau3AI GATC 2 cut(s) 67, 222
Sau96I GGNCC 2 cut(s) 43, 137
ScrFI CCNGG 2 cut(s) 135, 296
SduI GDGCHC 1 cut(s) 217
SetI ASST 9 cut(s) 32, 48, 61, 114, 124, 136, 166, 217, 332
SinI GGWCC 1 cut(s) 43
Sse9I AATT 2 cut(s) 16, 38
SsiI CCGC 3 cut(s) 256, 277, 352
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 2 cut(s) 133, 294
TaqI TCGA 2 cut(s) 48, 217
TasI AATT 2 cut(s) 16, 38
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 1 cut(s) 115
TseI GCWGC 1 cut(s) 253
VpaK11BI GGWCC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.