MD03G1276900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028495
Physical Location & Seq
Forward (+)
35949025 .. 35949514
490 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1276900.v1.1.491

Sequence Viewer

Length: 348 bp
ATGGCACCTGGCGGCGGCGGAGGAGGCGGTGGTGGAGGAGGGGGTGGAGGTGGTGGTGGTGGAGGTGGAGGTGGAGGTGGTGGAGGTGGCGGTGGAGGAGGAGGGCCTGTAGGCGGAGGAGGAGGGCCTGTAGGCGGAGGAGGAGGGCCTGGAGGCGGCGGAAGAGCACATGTCAATGTCGCAGGAGAATCTAGCGGCGCTGCAGGAGTTGACAGTAGTGCTACTTGGTGTTCTTGGATCTTGTGCTGCTGTGGTTGCTTCAATGATACTCCCACTAAGCCTAAGGATGACACTCCCGATAAGCCTGGCGATGATACTGCCTCGAAGCCTCTTCTCGTCACTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

9.71

Weight (kDa)

4.6

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028495)

Species Orthologous Gene IDs
malus_domestica MD03G1276900.v1.1
pyrus_communis pycom03g22060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AclWI GGATC 1 cut(s) 245
AfiI CCNNNNNNNGG 4 cut(s) 14, 113, 134, 155
AflIII ACRYGT 1 cut(s) 169
AgsI TTSAA 1 cut(s) 262
AjnI CCWGG 3 cut(s) 7, 148, 304
Alw21I GWGCWC 1 cut(s) 169
AlwI GGATC 1 cut(s) 245
AoxI GGCC 3 cut(s) 104, 125, 146
ApeKI GCWGC 2 cut(s) 200, 246
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 3 cut(s) 104, 125, 146
AxyI CCTNAGG 1 cut(s) 282
BanI GGYRCC 1 cut(s) 4
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 187, 233
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 3 cut(s) 9, 150, 306
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 3 cut(s) 108, 129, 201
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 6 cut(s) 13, 16, 157, 196, 201, 247
BlsI GCNGC 6 cut(s) 14, 17, 158, 197, 202, 248
Bme1390I CCNGG 3 cut(s) 9, 150, 306
BmgT120I GGNCC 3 cut(s) 104, 125, 146
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 9, 150, 306
BpmI CTGGAG 1 cut(s) 171
BsaAI YACGTR 1 cut(s) 345
BsaXI ACNNNNNCTCC 4 cut(s) 93, 114, 123, 144
Bsc4I CCNNNNNNNGG 4 cut(s) 14, 113, 134, 155
Bse21I CCTNAGG 1 cut(s) 282
BseBI CCWGG 3 cut(s) 9, 150, 306
BseGI GGATG 1 cut(s) 292
BseLI CCNNNNNNNGG 4 cut(s) 14, 113, 134, 155
BseRI GAGGAG 8 cut(s) 36, 51, 111, 114, 132, 135, 153, 156
BseXI GCAGC 2 cut(s) 187, 233
BshFI GGCC 3 cut(s) 106, 127, 148
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 169
BslI CCNNNNNNNGG 4 cut(s) 14, 113, 134, 155
BsnI GGCC 3 cut(s) 106, 127, 148
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 237
BspANI GGCC 3 cut(s) 106, 127, 148
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 205
BspPI GGATC 1 cut(s) 245
BspQI GCTCTTC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 4
BssMI GATC 1 cut(s) 237
Bst2UI CCWGG 3 cut(s) 9, 150, 306
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 2 cut(s) 157, 336
BstBAI YACGTR 1 cut(s) 345
BstDEI CTNAG 2 cut(s) 276, 282
BstF5I GGATG 1 cut(s) 292
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 1 cut(s) 240
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 2 cut(s) 24, 255
BstNI CCWGG 3 cut(s) 9, 150, 306
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 7, 148, 304
BstSFI CTRYAG 3 cut(s) 108, 129, 201
BstSNI TACGTA 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 187, 233
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 3 cut(s) 106, 127, 148
BtgZI GCGATG 1 cut(s) 324
BtsCI GGATG 1 cut(s) 292
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 3 cut(s) 104, 125, 146
CviAII CATG 1 cut(s) 170
CviJI RGCY 6 cut(s) 106, 127, 148, 280, 304, 328
CviKI_1 RGCY 6 cut(s) 106, 127, 148, 280, 304, 328
DdeI CTNAG 2 cut(s) 276, 282
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
Eam1104I CTCTTC 2 cut(s) 157, 336
EarI CTCTTC 2 cut(s) 157, 336
EciI GGCGGA 4 cut(s) 33, 129, 150, 174
Eco105I TACGTA 1 cut(s) 345
Eco81I CCTNAGG 1 cut(s) 282
EcoO109I RGGNCCY 3 cut(s) 104, 125, 146
EcoRII CCWGG 3 cut(s) 7, 148, 304
FaeI CATG 1 cut(s) 173
FaiI YATR 1 cut(s) 171
FatI CATG 1 cut(s) 169
Fnu4HI GCNGC 6 cut(s) 13, 16, 157, 196, 201, 247
FokI GGATG 1 cut(s) 299
Fsp4HI GCNGC 6 cut(s) 13, 16, 157, 196, 201, 247
FspBI CTAG 1 cut(s) 192
GlaI GCGC 1 cut(s) 199
GluI GCNGC 6 cut(s) 13, 16, 157, 196, 201, 247
GsuI CTGGAG 1 cut(s) 171
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 3 cut(s) 106, 127, 148
HhaI GCGC 1 cut(s) 200
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HinfI GANTC 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 1 cut(s) 211
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 1 cut(s) 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 255
HpyF3I CTNAG 2 cut(s) 276, 282
HpySE526I ACGT 1 cut(s) 344
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 237
LguI GCTCTTC 1 cut(s) 157
LpnPI CCDG 9 cut(s) 21, 120, 135, 141, 162, 168, 189, 291, 318
Lsp1109I GCAGC 2 cut(s) 187, 233
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 337
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 2 cut(s) 174, 323
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 169
MslI CAYNNNNRTG 1 cut(s) 174
MspR9I CCNGG 3 cut(s) 9, 150, 306
MvaI CCWGG 3 cut(s) 9, 150, 306
MwoI GCNNNNNNNGC 2 cut(s) 24, 255
NdeII GATC 1 cut(s) 237
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 1 cut(s) 337
NspI RCATGY 1 cut(s) 173
PciI ACATGT 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 6 cut(s) 14, 17, 158, 197, 202, 248
Ppu21I YACGTR 1 cut(s) 345
PscI ACATGT 1 cut(s) 169
Psp6I CCWGG 3 cut(s) 7, 148, 304
PspGI CCWGG 3 cut(s) 7, 148, 304
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 3 cut(s) 104, 125, 146
PstI CTGCAG 1 cut(s) 205
PsuI RGATCY 1 cut(s) 237
RseI CAYNNNNRTG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 157
SatI GCNGC 6 cut(s) 13, 16, 157, 196, 201, 247
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 3 cut(s) 104, 125, 146
ScrFI CCNGG 3 cut(s) 9, 150, 306
SduI GDGCHC 1 cut(s) 169
SetI ASST 7 cut(s) 10, 52, 67, 73, 79, 88, 347
SfcI CTRYAG 3 cut(s) 108, 129, 201
SmiMI CAYNNNNRTG 1 cut(s) 174
SnaBI TACGTA 1 cut(s) 345
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 7, 148, 304
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 1 cut(s) 347
TaqI TCGA 1 cut(s) 323
TauI GCSGC 4 cut(s) 15, 18, 159, 198
TfiI GAWTC 1 cut(s) 188
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 2 cut(s) 200, 246
Tsp45I GTSAC 1 cut(s) 337
XceI RCATGY 1 cut(s) 173
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.