MD03G1294300.v1.1

Protein trichome birefringence-like 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0028502
Physical Location & Seq
Reverse (-)
37052485 .. 37059570
7086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1294300.v1.1.491

Sequence Viewer

Length: 3390 bp
ATGAAGAGGCCGCAGCTCAGTATTTTTTCCATTACATTACCAACTACATCCTTAGTTTTTTATTTTAGCATCGCTTCCTCTATCATCTTTCTCTCTTTCGTTCTCATTTGGGTCAACATATCCTCTCCTTCCAACATTCCATTGCCCGCCCAACAAACCACCAATTGTCTCGAGTATTTCAACACATCCTCCATCGTTTTCGGTTTCACACCATCACCCTTAGCCGCCACAGTTCCACCTCTTCCAGCTGCTGATCATCCAACTAATTTAACTACCGGAGATGATTTTATCGATAAAAATAATGTTTCCGTTACAGAAAATAGTCCTTCGAAAGATTTATCAGTCTTGAAGTTGACGGACACCCCTTCCAGTACACCTGAAAATGCTTCTTCTAGCTATTTTGAGAACGAAGCCCAAGCAGTGGCAGTACTGCAACATGAGTTACATGATGACGAAGAAGAAGAAGAAGAAGAAGAAGAAGACAGCGGCAGCCGTGAGTTAGATATTATTTTTGATAGTAACTTCACAGCAGTGCCGGCACAACAAATTCCACCAGCTGCAATCTTAATTGACAAGATTGAGACAAATACTCATGACGACCAAAATGTTCCATCCGTGAACACGACTGTAGAGCAAAATGCTCCATTGAACCGCACTCATGAAGAGCAAACTGTGCCGTTGGCAAACACAACCGAGGAGCAAAATGCACCGTTGATGAACAAGACTGAAGAGCAAAATGCTCCAATTTTGATGAACAAGACGGAACAGCAAAATGCAGCTGCATTGAGTGAAAAAACGGTTCAAGCTAAGAGTAGAAAGAAGAAGAAGGCCAGAGCAACTTCATTTGATCAGATGACTCAAATTTCAAGTAGCGGACCGCTGTTGGTCGAAGAAGACAAGAAAATAGCTAAATTAGGAGGATGTGATTTTAGTACAAGAGGGAGGTGGGTCGAAGACGAGAGCTATCCGTTGTACACAAGTCGTTCATGTCCGTTCATAGAAGAAGGTTTTAGTTGTAATGGGAGATTAGACAAAAACTTCACTAAGTGGAGATGGCAACCACAAGATTGTGACATTCCAAGGTTCGACGCAAGAAAAATGCTTGAATTTATCAGAGGGAAGAGGCTAGTGTTTGTGGGCGATTCAATCAATAAGAATCAATGGCAATCTATGGTGTGCATGCTTGTCGGAGATAAAAGAAATCCAAATAAGCCCAGATTCATCAGAAAGAGAGGAGGCTATAGTGTCGTCTTTGTGGATTACCAGTGTAAAGTTGAATTCTATCCGTCCGTTTTCTTGGTTGAACAAGTCATGACAAGAGTAGACCAGAAGCAGGAGCAAACGTCCTTGCGAATTGATTCCATTGATCACGGTTCACATCGATGGAGAGAAGCTGATATTTTGGTATTCAACACCGAACATTGGTGGGTCGAATTCAAAACTAATGCCGGGGTTAATTTCTTCCAGGAAGGTGAAAAAATTCATCCATATCTTGATGTTCTCACAGCTCTCAGAAAGGGTTTGATGACGTGGGCATCATGGGTTGACAATTACATCAATCCAAGCAAAACTCAAGTTTTCTTTCGAACTTCATCGCCAACGCACTTCAGCGGAGGAAAATGGAATTTACGGCAGAGTTGTGTAGGAGTTAGTGAGCCCCTCCTCCAACCTTCAGGAATTGTAAACGAGAAAGATGTAATTGTAGAGGAGGTCCTAAAGCAGATGAAGACTCGAGTCACTTTGTTGAACACAACGAGCTTGTCAGAGTATCGAATGGATGCTCATCCATCCGACATCAGAGATTGCAGCCATTGGTGCCTTCCTGGCATTCCTGATGTATGGAATGAATTGTTATATCTTCATTTGAGGGTATCCCAAGCAGTGGGCGCACAAAATGATTTACATGTCAAAAATGAAGACGGAGGCGCCAGTGAGTCAGACATTTTTACTAACGTCACGGTAGTCCCGGCTCAAAGTGCACCAGCTGCAACCTTATTTGACAAGATTGAGAACCACAATGCTCCGTTGGTGAACACGACAAAAGAGCAAAATGAACCGACGTCATTAATGAACAAGACCGATCAAGAGCAAAGTGCGCCAATATCGGAAAACAGGACTGATCAAGAGCAAACTGCTGCATTGATGAACAAGATTGAGAGGGAGAGTGTAGTCCAAAATGCTCCAATATTGATGAATAAGGCTGATCAAGAGCAAAACCCTCCAACATTGATGAACAAGACTACTGACGAGAAAAACGCTGCATTGGTTGAAAAACCGATAAAAAAGAGGATGGCGGGAAAGAGGAAGAAGAAGAAGAGCATAGCAACATCATTTGATCAAAAGAGTAGTACTACTCAAATTTCAAGTAGCGGCGGGATGCAAGACATTGAAGAAAACAAGAAGAGAACAACAAAATTAGGAGGATGTGATTTTAGTACAAGAGGGAGGTGGGTCTATGATGAAAGCTATCCGTTGTACACACGTCGATCCTGTCCCTTCATATATGAAGGTTTTGATTGTCAAGGCAGTGGGAGATTAGATAAAAACTTCACCAAGTGGAGGTGGCAACCACAAGATTGTGAAATTCCAAGGTTCAATGCAACAAAAATGCTGGAACTGATGAGAGGTAAACGGCTACTTTATGTGGGGGATTCAATCAACAGAAATCAATTTGAATCCATGGTGTGCATGCTAATGGGAGATATAAGAGATCCCAATAGGCCCAGAATCTCCCATGCCAAGGGGACTTACAGTTTTTACTTTGTGGATTACAAGTTTACAGTCGAGTTTTCTCCATCCGTTTTCTTGGTTGAGAAAGGCACAAAAAGAGTAGGGCAGAAGCAGGTGCAAACGTCCTTGCGAATTGATGCCATTGATCAGGGTTCATCTAGATGGAGAGGAGCTGATATTTTGGTATTCGACACTGAACATTGGTGGACCGATTCCAAAACTAACTCTGGGAAGAATTTTTACCAGGAAGGGGGAAAAATTCATCCATATCTTGATGTTCACACAGCTCTCAGAAAAGGTTTGATGACCTGGGCAGCATGGGTTGATAAAAGTATCAATCCACACAAAACCCAAGTTTTCTTTCGAACTTCATCACCAACGCATTTCAGCGGAGGTGAGTGGAAATTGAGCCGGAGTTGTATAGGAGCTACTGAACCCCTCCCCAAACCTTCAGGAATGGCAAACAAGAAAGATGTAGTCCTAGAGGAGGTCGTAAAGCAGATGAAAACTCATGTGACATTGTTGAACATAACAAGCCTGTCAGAATATAGAATAGATGCTCATCCAACAGCCATCAAAGACTGCAGCCATTGGTGTCTTCCTGGCCTGCCTGATGCCTGGAATGAATTGTTGTTTCTGCATTTGCTCAGTGACTCATGA

Protein Analysis

1130

Amino Acids

128.03

Weight (kDa)

6.1

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 308 - 358 3.5e-17 PMR5 N terminal Domain
PC-Esterase PF13839 359 - 619 8.9e-84 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
PMR5N PF14416 809 - 861 4.3e-19 PMR5 N terminal Domain
PC-Esterase PF13839 862 - 1122 2.8e-85 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028502)

Species Orthologous Gene IDs
malus_domestica MD03G1294300.v1.1
pyrus_communis pycom03g23260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2833
AatII GACGTC 1 cut(s) 2063
Acc36I ACCTGC 1 cut(s) 2833
AccB1I GGYRCC 2 cut(s) 1815, 1925
AccB7I CCANNNNNTGG 2 cut(s) 421, 1882
AccI GTMKAC 1 cut(s) 1323
AclWI GGATC 2 cut(s) 2481, 2705
AcuI CTGAAG 4 cut(s) 747, 1591, 1656, 3165
AcyI GRCGYC 2 cut(s) 1926, 2060
AdeI CACNNNGTG 2 cut(s) 1047, 2556
AfaI GTAC 7 cut(s) 373, 429, 934, 974, 2350, 2437, 2477
AfiI CCNNNNNNNGG 8 cut(s) 421, 1333, 1422, 1882, 2559, 2740, 2980, 3217
AflIII ACRYGT 2 cut(s) 1903, 2480
AjiI CACGTC 2 cut(s) 1530, 2483
AjnI CCWGG 6 cut(s) 1464, 1822, 2973, 3038, 3331, 3347
AjuI GAANNNNNNNTTGG 2 cut(s) 3075, 3107
AleI CACNNNNGTG 1 cut(s) 530
Alw21I GWGCWC 1 cut(s) 1981
Alw26I GTCTC 2 cut(s) 173, 575
Alw44I GTGCAC 1 cut(s) 1977
AlwI GGATC 2 cut(s) 2481, 2705
AlwNI CAGNNNCTG 1 cut(s) 251
Ama87I CYCGRG 2 cut(s) 170, 1731
AoxI GGCC 4 cut(s) 8, 828, 2720, 3334
ApaLI GTGCAC 1 cut(s) 1977
AseI ATTAAT 1 cut(s) 2066
Asp700I GAANNNNTTC 2 cut(s) 1357, 1479
AspLEI GCGC 3 cut(s) 1889, 1928, 2098
AspS9I GGNCC 4 cut(s) 875, 1711, 2721, 2937
AsuC2I CCSGG 2 cut(s) 1450, 1967
AsuHPI GGTGA 6 cut(s) 207, 1484, 2041, 2542, 3096, 3137
AsuII TTCGAA 3 cut(s) 329, 1585, 3094
AvaI CYCGRG 2 cut(s) 170, 1731
AvaII GGWCC 3 cut(s) 875, 1711, 2937
BaeGI GKGCMC 1 cut(s) 1981
BanI GGYRCC 2 cut(s) 1815, 1925
BanII GRGCYC 1 cut(s) 1659
BarI GAAGNNNNNNTAC 2 cut(s) 2954, 2986
BbsI GAAGAC 6 cut(s) 486, 900, 960, 1733, 1923, 3320
Bbv12I GWGCWC 1 cut(s) 1981
BceAI ACGGC 4 cut(s) 477, 661, 1646, 2648
BcgI CGANNNNNNTGC 2 cut(s) 1168, 1202
BciT130I CCWGG 6 cut(s) 1466, 1824, 2975, 3040, 3333, 3349
BciVI GTATCC 1 cut(s) 1882
BclI TGATCA 7 cut(s) 253, 847, 1366, 2119, 2203, 2335, 2875
BcnI CCSGG 2 cut(s) 1450, 1967
BcoDI GTCTC 2 cut(s) 173, 575
BfaI CTAG 4 cut(s) 393, 1127, 2889, 3212
BfmI CTRYAG 3 cut(s) 627, 1240, 3313
BfoI RGCGCY 1 cut(s) 1929
BfuAI ACCTGC 1 cut(s) 2833
BfuI GTATCC 1 cut(s) 1882
BglI GCCNNNNNGGC 1 cut(s) 1824
BmcAI AGTACT 2 cut(s) 429, 2350
Bme1390I CCNGG 8 cut(s) 1450, 1466, 1824, 1967, 2975, 3040, 3333, 3349
Bme18I GGWCC 3 cut(s) 875, 1711, 2937
BmeT110I CYCGRG 2 cut(s) 170, 1731
BmgBI CACGTC 2 cut(s) 1530, 2483
BmgT120I GGNCC 4 cut(s) 875, 1711, 2721, 2937
BmiI GGNNCC 2 cut(s) 1817, 1927
BmrFI CCNGG 8 cut(s) 1450, 1466, 1824, 1967, 2975, 3040, 3333, 3349
BmsI GCATC 7 cut(s) 78, 1544, 1768, 2367, 2857, 3277, 3334
BoxI GACNNNNGTC 1 cut(s) 1733
BpiI GAAGAC 6 cut(s) 486, 900, 960, 1733, 1923, 3320
Bpu10I CCTNAGC 1 cut(s) 220
Bpu14I TTCGAA 3 cut(s) 329, 1585, 3094
BpuEI CTTGAG 1 cut(s) 1557
BpuMI CCSGG 2 cut(s) 1450, 1967
Bsa29I ATCGAT 2 cut(s) 291, 1381
BsaBI GATNNNNATC 2 cut(s) 1782, 3291
BsaHI GRCGYC 2 cut(s) 1926, 2060
BsaJI CCNNGG 7 cut(s) 693, 1079, 1449, 2588, 2679, 2739, 3039
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 4 cut(s) 173, 203, 1328, 1358
Bsc4I CCNNNNNNNGG 8 cut(s) 421, 1333, 1422, 1882, 2559, 2740, 2980, 3217
Bse118I RCCGGY 1 cut(s) 535
Bse1I ACTGG 3 cut(s) 369, 1264, 1929
Bse3DI GCAATG 1 cut(s) 140
Bse8I GATNNNNATC 2 cut(s) 1782, 3291
BseBI CCWGG 6 cut(s) 1466, 1824, 2975, 3040, 3333, 3349
BseCI ATCGAT 2 cut(s) 291, 1381
BseDI CCNNGG 7 cut(s) 693, 1079, 1449, 2588, 2679, 2739, 3039
BseJI GATNNNNATC 2 cut(s) 1782, 3291
BseLI CCNNNNNNNGG 8 cut(s) 421, 1333, 1422, 1882, 2559, 2740, 2980, 3217
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 3 cut(s) 31, 1525, 3034
BseNI ACTGG 3 cut(s) 369, 1264, 1929
BseRI GAGGAG 6 cut(s) 710, 1248, 1652, 1721, 2913, 3230
BseSI GKGCMC 1 cut(s) 1981
BshFI GGCC 4 cut(s) 10, 830, 2722, 3336
BshNI GGYRCC 2 cut(s) 1815, 1925
BshVI ATCGAT 2 cut(s) 291, 1381
BsiHKAI GWGCWC 1 cut(s) 1981
BsiHKCI CYCGRG 2 cut(s) 170, 1731
BsiSI CCGG 5 cut(s) 276, 536, 1449, 1967, 3142
BslFI GGGAC 3 cut(s) 1949, 2478, 2758
BslI CCNNNNNNNGG 8 cut(s) 421, 1333, 1422, 1882, 2559, 2740, 2980, 3217
BsmAI GTCTC 2 cut(s) 173, 575
BsmFI GGGAC 3 cut(s) 1949, 2478, 2758
BsmI GAATGC 1 cut(s) 1827
BsnI GGCC 4 cut(s) 10, 830, 2722, 3336
BsoBI CYCGRG 2 cut(s) 170, 1731
Bsp119I TTCGAA 3 cut(s) 329, 1585, 3094
Bsp1286I GDGCHC 2 cut(s) 1659, 1981
Bsp1407I TGTACA 2 cut(s) 972, 2475
Bsp19I CCATGG 1 cut(s) 2679
BspANI GGCC 4 cut(s) 10, 830, 2722, 3336
BspCNI CTCAG 4 cut(s) 30, 1524, 3033, 3390
BspDI ATCGAT 2 cut(s) 291, 1381
BspHI TCATGA 4 cut(s) 592, 658, 1311, 3386
BspLI GGNNCC 2 cut(s) 1817, 1927
BspMAI CTGCAG 1 cut(s) 3317
BspMI ACCTGC 1 cut(s) 2833
BspPI GGATC 2 cut(s) 2481, 2705
BspQI GCTCTTC 3 cut(s) 657, 723, 2309
BspT104I TTCGAA 3 cut(s) 329, 1585, 3094
BspT107I GGYRCC 2 cut(s) 1815, 1925
BsrDI GCAATG 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 535
BsrGI TGTACA 2 cut(s) 972, 2475
BsrI ACTGG 3 cut(s) 369, 1264, 1929
BssAI RCCGGY 1 cut(s) 535
BssECI CCNNGG 7 cut(s) 693, 1079, 1449, 2588, 2679, 2739, 3039
BssNI GRCGYC 2 cut(s) 1926, 2060
BssT1I CCWWGG 4 cut(s) 1079, 2588, 2679, 2739
Bst2UI CCWGG 6 cut(s) 1466, 1824, 2975, 3040, 3333, 3349
Bst4CI ACNGT 9 cut(s) 232, 628, 673, 711, 799, 1373, 1960, 2753, 2782
Bst6I CTCTTC 6 cut(s) 246, 657, 723, 1115, 2309, 2396
BstACI GRCGYC 2 cut(s) 1926, 2060
BstAPI GCANNNNNTGC 2 cut(s) 673, 1985
BstAUI TGTACA 2 cut(s) 972, 2475
BstBI TTCGAA 3 cut(s) 329, 1585, 3094
BstC8I GCNNGC 5 cut(s) 147, 537, 1181, 2690, 3338
BstDEI CTNAG 8 cut(s) 17, 52, 220, 807, 1044, 1511, 3020, 3377
BstDSI CCRYGG 1 cut(s) 2679
BstENI CCTNNNNNAGG 1 cut(s) 3215
BstH2I RGCGCY 1 cut(s) 1929
BstHHI GCGC 3 cut(s) 1889, 1928, 2098
BstMAI GTCTC 2 cut(s) 173, 575
BstMWI GCNNNNNNNGC 8 cut(s) 536, 673, 1815, 1824, 1886, 1976, 1985, 2095
BstNI CCWGG 6 cut(s) 1466, 1824, 2975, 3040, 3333, 3349
BstNSI RCATGY 3 cut(s) 1183, 1907, 2692
BstPAI GACNNNNGTC 1 cut(s) 1733
BstSCI CCNGG 8 cut(s) 1448, 1464, 1822, 1965, 2973, 3038, 3331, 3347
BstSFI CTRYAG 3 cut(s) 627, 1240, 3313
BstSLI GKGCMC 1 cut(s) 1981
BstV2I GAAGAC 6 cut(s) 486, 900, 960, 1733, 1923, 3320
BstX2I RGATCY 1 cut(s) 2710
BstYI RGATCY 1 cut(s) 2710
Bsu15I ATCGAT 2 cut(s) 291, 1381
BsuI GTATCC 1 cut(s) 1882
BsuRI GGCC 4 cut(s) 10, 830, 2722, 3336
BsuTUI ATCGAT 2 cut(s) 291, 1381
BtgI CCRYGG 1 cut(s) 2679
BtgZI GCGATG 2 cut(s) 55, 1578
BtrI CACGTC 2 cut(s) 1530, 2483
BtsI GCAGTG 4 cut(s) 426, 537, 1887, 2533
BtsIMutI CAGTG 8 cut(s) 426, 537, 1271, 1887, 1936, 2533, 2922, 3385
BveI ACCTGC 1 cut(s) 2833
Cac8I GCNNGC 5 cut(s) 147, 537, 1181, 2690, 3338
CaiI CAGNNNCTG 1 cut(s) 251
CciI TCATGA 4 cut(s) 592, 658, 1311, 3386
CfoI GCGC 3 cut(s) 1889, 1928, 2098
Cfr10I RCCGGY 1 cut(s) 535
Cfr13I GGNCC 4 cut(s) 875, 1711, 2721, 2937
ClaI ATCGAT 2 cut(s) 291, 1381
CpoI CGGWCCG 1 cut(s) 875
CseI GACGC 1 cut(s) 1097
Csp6I GTAC 7 cut(s) 372, 428, 933, 973, 2349, 2436, 2476
CspI CGGWCCG 1 cut(s) 875
CviQI GTAC 7 cut(s) 372, 428, 933, 973, 2349, 2436, 2476
DdeI CTNAG 8 cut(s) 17, 52, 220, 807, 1044, 1511, 3020, 3377
DinI GGCGCC 1 cut(s) 1927
DraIII CACNNNGTG 2 cut(s) 1047, 2556
Eam1104I CTCTTC 6 cut(s) 246, 657, 723, 1115, 2309, 2396
EarI CTCTTC 6 cut(s) 246, 657, 723, 1115, 2309, 2396
Eco130I CCWWGG 4 cut(s) 1079, 2588, 2679, 2739
Eco24I GRGCYC 1 cut(s) 1659
Eco47I GGWCC 3 cut(s) 875, 1711, 2937
Eco57I CTGAAG 4 cut(s) 747, 1591, 1656, 3165
Eco88I CYCGRG 2 cut(s) 170, 1731
EcoNI CCTNNNNNAGG 1 cut(s) 3215
EcoO109I RGGNCCY 1 cut(s) 1711
EcoRI GAATTC 2 cut(s) 1277, 1433
EcoRII CCWGG 6 cut(s) 1464, 1822, 2973, 3038, 3331, 3347
EcoT14I CCWWGG 4 cut(s) 1079, 2588, 2679, 2739
EcoT38I GRGCYC 1 cut(s) 1659
EgeI GGCGCC 1 cut(s) 1927
EheI GGCGCC 1 cut(s) 1927
ErhI CCWWGG 4 cut(s) 1079, 2588, 2679, 2739
FaqI GGGAC 3 cut(s) 1949, 2478, 2758
FauI CCCGC 3 cut(s) 154, 2287, 2366
FbaI TGATCA 7 cut(s) 253, 847, 1366, 2119, 2203, 2335, 2875
FblI GTMKAC 1 cut(s) 1323
FriOI GRGCYC 1 cut(s) 1659
FspBI CTAG 4 cut(s) 393, 1127, 2889, 3212
GlaI GCGC 3 cut(s) 1888, 1927, 2097
HaeII RGCGCY 1 cut(s) 1929
HaeIII GGCC 4 cut(s) 10, 830, 2722, 3336
HapII CCGG 5 cut(s) 276, 536, 1449, 1967, 3142
HgaI GACGC 1 cut(s) 1097
HhaI GCGC 3 cut(s) 1889, 1928, 2098
Hin1I GRCGYC 2 cut(s) 1926, 2060
Hin6I GCGC 3 cut(s) 1887, 1926, 2096
HinP1I GCGC 3 cut(s) 1887, 1926, 2096
HincII GTYRAC 3 cut(s) 115, 354, 1546
HindII GTYRAC 3 cut(s) 115, 354, 1546
HpaII CCGG 5 cut(s) 276, 536, 1449, 1967, 3142
HphI GGTGA 6 cut(s) 207, 1484, 2041, 2542, 3096, 3137
Hpy99I CGWCG 3 cut(s) 1091, 2062, 2487
HpyCH4III ACNGT 9 cut(s) 232, 628, 673, 711, 799, 1373, 1960, 2753, 2782
HpyCH4IV ACGT 6 cut(s) 1343, 1529, 1953, 2060, 2482, 2852
HpyF10VI GCNNNNNNNGC 8 cut(s) 536, 673, 1815, 1824, 1886, 1976, 1985, 2095
HpyF3I CTNAG 8 cut(s) 17, 52, 220, 807, 1044, 1511, 3020, 3377
HpySE526I ACGT 6 cut(s) 1343, 1529, 1953, 2060, 2482, 2852
Hsp92I GRCGYC 2 cut(s) 1926, 2060
HspAI GCGC 3 cut(s) 1887, 1926, 2096
KasI GGCGCC 1 cut(s) 1925
KroI GCCGGC 1 cut(s) 535
KroNI GCCGGC 1 cut(s) 537
Ksp22I TGATCA 7 cut(s) 253, 847, 1366, 2119, 2203, 2335, 2875
LguI GCTCTTC 3 cut(s) 657, 723, 2309
LmnI GCTCC 8 cut(s) 646, 697, 745, 1336, 2026, 2185, 2900, 3155
LweI GCATC 7 cut(s) 78, 1544, 1768, 2367, 2857, 3277, 3334
MaeI CTAG 4 cut(s) 393, 1127, 2889, 3212
MaeII ACGT 6 cut(s) 1343, 1529, 1953, 2060, 2482, 2852
MaeIII GTNAC 8 cut(s) 310, 441, 518, 1070, 1735, 1954, 3244, 3380
MfeI CAATTG 1 cut(s) 163
MflI RGATCY 1 cut(s) 2710
MhlI GDGCHC 2 cut(s) 1659, 1981
Mly113I GGCGCC 1 cut(s) 1926
MlyI GAGTC 5 cut(s) 850, 1723, 1743, 1943, 3377
MmeI TCCRAC 7 cut(s) 156, 284, 1168, 1690, 1815, 2246, 3320
MroNI GCCGGC 1 cut(s) 535
MroXI GAANNNNTTC 2 cut(s) 1357, 1479
MseI TTAA 4 cut(s) 269, 566, 1455, 2066
MslI CAYNNNNRTG 4 cut(s) 530, 1381, 2693, 2890
MspA1I CMGCKG 8 cut(s) 248, 486, 557, 779, 880, 1611, 1985, 3120
MspI CCGG 5 cut(s) 276, 536, 1449, 1967, 3142
MspR9I CCNGG 8 cut(s) 1450, 1466, 1824, 1967, 2975, 3040, 3333, 3349
MunI CAATTG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 1827
MvaI CCWGG 6 cut(s) 1466, 1824, 2975, 3040, 3333, 3349
MwoI GCNNNNNNNGC 8 cut(s) 536, 673, 1815, 1824, 1886, 1976, 1985, 2095
NaeI GCCGGC 1 cut(s) 537
NarI GGCGCC 1 cut(s) 1926
NciI CCSGG 2 cut(s) 1450, 1967
NcoI CCATGG 1 cut(s) 2679
NgoMIV GCCGGC 1 cut(s) 535
NlaIV GGNNCC 2 cut(s) 1817, 1927
NmuCI GTSAC 5 cut(s) 1070, 1735, 1954, 3244, 3380
NspI RCATGY 3 cut(s) 1183, 1907, 2692
NspV TTCGAA 3 cut(s) 329, 1585, 3094
OliI CACNNNNGTG 1 cut(s) 530
PaeI GCATGC 2 cut(s) 1183, 2692
PaeR7I CTCGAG 2 cut(s) 170, 1731
PagI TCATGA 4 cut(s) 592, 658, 1311, 3386
PaqCI CACCTGC 1 cut(s) 2833
PciI ACATGT 1 cut(s) 1903
PciSI GCTCTTC 3 cut(s) 657, 723, 2309
PctI GAATGC 1 cut(s) 1827
PdiI GCCGGC 1 cut(s) 537
PdmI GAANNNNTTC 2 cut(s) 1357, 1479
PfeI GAWTC 8 cut(s) 1142, 1156, 1218, 1358, 2651, 2675, 2727, 2942
PflMI CCANNNNNTGG 2 cut(s) 421, 1882
PfoI TCCNGGA 1 cut(s) 1464
PleI GAGTC 5 cut(s) 850, 1723, 1742, 1942, 3377
PluTI GGCGCC 1 cut(s) 1929
PpsI GAGTC 5 cut(s) 850, 1723, 1742, 1942, 3377
PpuMI RGGWCCY 1 cut(s) 1711
PscI ACATGT 1 cut(s) 1903
PshAI GACNNNNGTC 1 cut(s) 1733
PshBI ATTAAT 1 cut(s) 2066
Psp5II RGGWCCY 1 cut(s) 1711
Psp6I CCWGG 6 cut(s) 1464, 1822, 2973, 3038, 3331, 3347
PspGI CCWGG 6 cut(s) 1464, 1822, 2973, 3038, 3331, 3347
PspN4I GGNNCC 2 cut(s) 1817, 1927
PspPI GGNCC 4 cut(s) 875, 1711, 2721, 2937
PspPPI RGGWCCY 1 cut(s) 1711
PspXI VCTCGAGB 1 cut(s) 1731
PstI CTGCAG 1 cut(s) 3317
PstNI CAGNNNCTG 1 cut(s) 251
PsuI RGATCY 1 cut(s) 2710
PvuII CAGCTG 4 cut(s) 248, 557, 779, 1985
RsaI GTAC 7 cut(s) 373, 429, 934, 974, 2350, 2437, 2477
RsaNI GTAC 7 cut(s) 372, 428, 933, 973, 2349, 2436, 2476
RseI CAYNNNNRTG 4 cut(s) 530, 1381, 2693, 2890
Rsr2I CGGWCCG 1 cut(s) 875
RsrII CGGWCCG 1 cut(s) 875
SapI GCTCTTC 3 cut(s) 657, 723, 2309
SaqAI TTAA 4 cut(s) 269, 566, 1455, 2066
Sau96I GGNCC 4 cut(s) 875, 1711, 2721, 2937
ScaI AGTACT 2 cut(s) 429, 2350
SchI GAGTC 5 cut(s) 850, 1723, 1743, 1943, 3377
ScrFI CCNGG 8 cut(s) 1450, 1466, 1824, 1967, 2975, 3040, 3333, 3349
SduI GDGCHC 2 cut(s) 1659, 1981
SfaNI GCATC 7 cut(s) 78, 1544, 1768, 2367, 2857, 3277, 3334
SfcI CTRYAG 3 cut(s) 627, 1240, 3313
SfoI GGCGCC 1 cut(s) 1927
Sfr274I CTCGAG 2 cut(s) 170, 1731
SfuI TTCGAA 3 cut(s) 329, 1585, 3094
SinI GGWCC 3 cut(s) 875, 1711, 2937
SlaI CTCGAG 2 cut(s) 170, 1731
SmiMI CAYNNNNRTG 4 cut(s) 530, 1381, 2693, 2890
SmlI CTYRAG 3 cut(s) 170, 1572, 1731
SmoI CTYRAG 3 cut(s) 170, 1572, 1731
SphI GCATGC 2 cut(s) 1183, 2692
SspDI GGCGCC 1 cut(s) 1925
SspI AATATT 1 cut(s) 2187
SspMI CTAG 4 cut(s) 393, 1127, 2889, 3212
StyD4I CCNGG 8 cut(s) 1448, 1464, 1822, 1965, 2973, 3038, 3331, 3347
StyI CCWWGG 4 cut(s) 1079, 2588, 2679, 2739
TaaI ACNGT 9 cut(s) 232, 628, 673, 711, 799, 1373, 1960, 2753, 2782
TaiI ACGT 6 cut(s) 1346, 1532, 1956, 2063, 2485, 2855
TaqII GACCGA 2 cut(s) 2093, 2954
TatI WGTACW 7 cut(s) 371, 427, 932, 972, 2348, 2435, 2475
TauI GCSGC 4 cut(s) 13, 227, 489, 2373
TfiI GAWTC 8 cut(s) 1142, 1156, 1218, 1358, 2651, 2675, 2727, 2942
Tru1I TTAA 4 cut(s) 269, 566, 1455, 2066
Tru9I TTAA 4 cut(s) 269, 566, 1455, 2066
TscAI CASTG 8 cut(s) 426, 537, 1271, 1887, 1936, 2533, 2929, 3385
TseFI GTSAC 5 cut(s) 1070, 1735, 1954, 3244, 3380
Tsp45I GTSAC 5 cut(s) 1070, 1735, 1954, 3244, 3380
TspRI CASTG 8 cut(s) 426, 537, 1271, 1887, 1936, 2533, 2929, 3385
Van91I CCANNNNNTGG 2 cut(s) 421, 1882
VneI GTGCAC 1 cut(s) 1977
VpaK11BI GGWCC 3 cut(s) 875, 1711, 2937
VspI ATTAAT 1 cut(s) 2066
XagI CCTNNNNNAGG 1 cut(s) 3215
XbaI TCTAGA 1 cut(s) 2888
XceI RCATGY 3 cut(s) 1183, 1907, 2692
XhoI CTCGAG 2 cut(s) 170, 1731
XmiI GTMKAC 1 cut(s) 1323
XmnI GAANNNNTTC 2 cut(s) 1357, 1479
XspI CTAG 4 cut(s) 393, 1127, 2889, 3212
ZraI GACGTC 1 cut(s) 2061
ZrmI AGTACT 2 cut(s) 429, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.