MD04G1050700.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0028684
Physical Location & Seq
Forward (+)
5948280 .. 5948792
513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1050700.v1.1.491

Sequence Viewer

Length: 513 bp
ATGGGAGCCGTAACGGAGAGCTTAGGCCTAAGTCCAACCTACTTGAGAAACAGTCTTGAGCAAGGAATGCACATCCTAGTAGCTAACACTTACCCGCAGTGCTCTCCAACTAGCAAGATTCTTGGATTACCACCACATACAGACCACAGCATCATCACCTTCCTTCTCGAAAGTACGTCAGGCCTTGAGATCATGGATTTCACAGACAGTAGTGCATGGAAGTCCATCCCGGCGGTGAAGGGTACGCTTAAGGTCATTGTTGGGAGCCATCTTGAAGTGCTCAGCAATGGAATGTACAAGAGTGTGTTCCACCGGGCGACTCCAAGCCCTCGATTGAGTAGGGTTTCTGTAGCCAGCTTCCTTAGCTTAAGAATGGGAGAGACAGTGGAGCCTGCTGTGGAGCTGGTTGATGAAGAGCACCCTCAAAAATATAGGGCAACTAGCCTCGATGAATTTTTCAACCATCTATCCTCTAACGAAGAAAGACCATACATAGACTGCCTTAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

171

Amino Acids

18.79

Weight (kDa)

5.77

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 24 - 121 2.2e-22 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028684)

Species Orthologous Gene IDs
malus_domestica MD04G1050700.v1.1
prunus_persica Prupe.5G059400_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 95, 233
AcsI RAATTY 1 cut(s) 452
AfaI GTAC 3 cut(s) 175, 244, 296
AflII CTTAAG 3 cut(s) 248, 367, 503
AgsI TTSAA 2 cut(s) 275, 460
AluBI AGCT 5 cut(s) 21, 83, 357, 366, 403
AluI AGCT 5 cut(s) 21, 83, 357, 366, 403
Alw21I GWGCWC 3 cut(s) 104, 282, 420
Alw26I GTCTC 1 cut(s) 374
AoxI GGCC 2 cut(s) 25, 181
ApoI RAATTY 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 230, 314
AsuHPI GGTGA 2 cut(s) 148, 247
Bbv12I GWGCWC 3 cut(s) 104, 282, 420
BccI CCATC 3 cut(s) 233, 276, 471
BcnI CCSGG 2 cut(s) 230, 314
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 3 cut(s) 77, 111, 441
BfmI CTRYAG 1 cut(s) 348
BfrI CTTAAG 3 cut(s) 248, 367, 503
BlpI GCTNAGC 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 230, 314
BmiI GGNNCC 3 cut(s) 7, 266, 390
BmrFI CCNGG 2 cut(s) 230, 314
BmsI GCATC 1 cut(s) 159
Bpu10I CCTNAGC 2 cut(s) 22, 362
Bpu1102I GCTNAGC 1 cut(s) 281
BpuEI CTTGAG 3 cut(s) 64, 77, 206
BpuMI CCSGG 2 cut(s) 230, 314
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bse3DI GCAATG 1 cut(s) 292
BseGI GGATG 2 cut(s) 72, 225
BseMI GCAATG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 295
BshFI GGCC 2 cut(s) 27, 183
BsiHKAI GWGCWC 3 cut(s) 104, 282, 420
BsiSI CCGG 2 cut(s) 230, 313
BsmAI GTCTC 1 cut(s) 374
BsmI GAATGC 1 cut(s) 72
BsnI GGCC 2 cut(s) 27, 183
Bsp1286I GDGCHC 3 cut(s) 104, 282, 420
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 1 cut(s) 189
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 2 cut(s) 95, 233
BspANI GGCC 2 cut(s) 27, 183
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 7, 266, 390
BspQI GCTCTTC 1 cut(s) 408
BspTI CTTAAG 3 cut(s) 248, 367, 503
BsrDI GCAATG 1 cut(s) 292
BsrGI TGTACA 1 cut(s) 294
BssMI GATC 1 cut(s) 189
Bst4CI ACNGT 3 cut(s) 53, 209, 385
Bst6I CTCTTC 1 cut(s) 408
BstAFI CTTAAG 3 cut(s) 248, 367, 503
BstAPI GCANNNNNTGC 1 cut(s) 67
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 355, 393
BstDEI CTNAG 4 cut(s) 22, 29, 281, 362
BstF5I GGATG 2 cut(s) 72, 225
BstKTI GATC 1 cut(s) 192
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 1 cut(s) 189
BstMWI GCNNNNNNNGC 2 cut(s) 67, 363
BstSCI CCNGG 2 cut(s) 228, 312
BstSFI CTRYAG 1 cut(s) 348
BsuRI GGCC 2 cut(s) 27, 183
BtsCI GGATG 2 cut(s) 72, 225
BtsI GCAGTG 1 cut(s) 104
BtsIMutI CAGTG 2 cut(s) 104, 390
Cac8I GCNNGC 2 cut(s) 355, 393
Csp6I GTAC 3 cut(s) 174, 243, 295
CviAII CATG 2 cut(s) 193, 216
CviQI GTAC 3 cut(s) 174, 243, 295
DdeI CTNAG 4 cut(s) 22, 29, 281, 362
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 408
EarI CTCTTC 1 cut(s) 408
Eco147I AGGCCT 2 cut(s) 27, 183
FaeI CATG 2 cut(s) 196, 219
FaiI YATR 6 cut(s) 138, 194, 217, 432, 490, 494
FatI CATG 2 cut(s) 192, 215
FauI CCCGC 1 cut(s) 102
FokI GGATG 2 cut(s) 59, 212
FspBI CTAG 3 cut(s) 77, 111, 441
HaeIII GGCC 2 cut(s) 27, 183
HapII CCGG 2 cut(s) 230, 313
Hin1II CATG 2 cut(s) 196, 219
HinfI GANTC 2 cut(s) 118, 319
HpaII CCGG 2 cut(s) 230, 313
HphI GGTGA 2 cut(s) 148, 247
Hpy188III TCNNGA 3 cut(s) 56, 167, 272
HpyAV CCTTC 3 cut(s) 169, 173, 232
HpyCH4III ACNGT 3 cut(s) 53, 209, 385
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 2 cut(s) 70, 215
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 363
HpyF3I CTNAG 4 cut(s) 22, 29, 281, 362
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 2 cut(s) 196, 219
Kzo9I GATC 1 cut(s) 189
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 4 cut(s) 5, 264, 388, 400
LpnPI CCDG 6 cut(s) 165, 243, 326, 367, 389, 405
LweI GCATC 1 cut(s) 159
MaeI CTAG 3 cut(s) 77, 111, 441
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 1 cut(s) 10
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 2 cut(s) 425, 491
MhlI GDGCHC 3 cut(s) 104, 282, 420
MluCI AATT 1 cut(s) 452
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 2 cut(s) 59, 131
MnlI CCTC 4 cut(s) 339, 432, 455, 481
MseI TTAA 3 cut(s) 249, 368, 504
MspCI CTTAAG 3 cut(s) 248, 367, 503
MspI CCGG 2 cut(s) 230, 313
MspR9I CCNGG 2 cut(s) 230, 314
Mva1269I GAATGC 1 cut(s) 72
MwoI GCNNNNNNNGC 2 cut(s) 67, 363
NciI CCSGG 2 cut(s) 230, 314
NdeII GATC 1 cut(s) 189
NlaIII CATG 2 cut(s) 196, 219
NlaIV GGNNCC 3 cut(s) 7, 266, 390
PceI AGGCCT 2 cut(s) 27, 183
PciSI GCTCTTC 1 cut(s) 408
PctI GAATGC 1 cut(s) 72
PfeI GAWTC 1 cut(s) 118
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 7, 266, 390
RsaI GTAC 3 cut(s) 175, 244, 296
RsaNI GTAC 3 cut(s) 174, 243, 295
SapI GCTCTTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 249, 368, 504
Sau3AI GATC 1 cut(s) 189
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 2 cut(s) 230, 314
SduI GDGCHC 3 cut(s) 104, 282, 420
SetI ASST 9 cut(s) 23, 41, 85, 161, 179, 255, 359, 368, 405
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 1 cut(s) 348
SmlI CTYRAG 6 cut(s) 43, 56, 185, 248, 367, 503
SmoI CTYRAG 6 cut(s) 43, 56, 185, 248, 367, 503
Sse9I AATT 1 cut(s) 452
SseBI AGGCCT 2 cut(s) 27, 183
SsiI CCGC 2 cut(s) 95, 233
SspMI CTAG 3 cut(s) 77, 111, 441
StuI AGGCCT 2 cut(s) 27, 183
StyD4I CCNGG 2 cut(s) 228, 312
TaaI ACNGT 3 cut(s) 53, 209, 385
TaiI ACGT 1 cut(s) 179
TaqI TCGA 3 cut(s) 168, 331, 447
TasI AATT 1 cut(s) 452
TatI WGTACW 1 cut(s) 294
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 3 cut(s) 249, 368, 504
Tru9I TTAA 3 cut(s) 249, 368, 504
TscAI CASTG 2 cut(s) 104, 390
TspDTI ATGAA 2 cut(s) 426, 465
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 2 cut(s) 104, 390
Vha464I CTTAAG 3 cut(s) 248, 367, 503
XapI RAATTY 1 cut(s) 452
XspI CTAG 3 cut(s) 77, 111, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.