MD04G1071600.v1.1

Peptidase inhibitor I9

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0028671
Physical Location & Seq
Reverse (-)
9697145 .. 9697595
451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1071600.v1.1.491

Sequence Viewer

Length: 393 bp
ATGTTTGGAGAGGCCAATGGCACAGCTACTGGCATGGCTCATCATGCTCACTTGGCAATGTACAAAGTCTCTGAGTTTGAGTGTGCTGAAGGTTATGTTTTGGCTGCAATGGATGTTGTGGTTGAGGATGGAGTGGACGTGCTATCCCTCTCAGTTGGCTGTCCCACAATTCCTTTCTCTGCAGCTGGAAACTCTGGTTTTGAACCCGGTTCTCTGTCAAATGAGCTCTCACGGATTCTCACAGTTGGAACAAGAACAACCGACATAATATTAAACATAAACTCACAACCTACAGCCACAATCTTGTCCAATGGCAACAGAGACGCACTTCATCCCGAGATCGCATCCTTTTCATCAAGAGGGCCAAACACTACAAGCCACGGAATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

131

Amino Acids

13.52

Weight (kDa)

4.79

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028671)

Species Orthologous Gene IDs
malus_domestica MD04G1071600.v1.1
pyrus_communis pycom04g06500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 108
AfaI GTAC 1 cut(s) 62
AgsI TTSAA 1 cut(s) 203
AjiI CACGTC 1 cut(s) 139
AluBI AGCT 3 cut(s) 26, 185, 226
AluI AGCT 3 cut(s) 26, 185, 226
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 73, 315
AlwNI CAGNNNCTG 1 cut(s) 29
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 12, 362
ApeKI GCWGC 2 cut(s) 104, 182
ApoI RAATTY 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 362
AsuC2I CCSGG 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 335
BanII GRGCYC 1 cut(s) 228
Bbv12I GWGCWC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 91, 194
BccI CCATC 1 cut(s) 122
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 2 cut(s) 73, 315
BfmI CTRYAG 2 cut(s) 180, 291
BisI GCNGC 2 cut(s) 105, 183
BlsI GCNGC 2 cut(s) 106, 184
Bme1390I CCNGG 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 362
BmrFI CCNGG 1 cut(s) 207
BmsI GCATC 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bse1I ACTGG 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 63, 114
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 4 cut(s) 118, 133, 331, 344
BseMI GCAATG 2 cut(s) 63, 114
BseMII CTCAG 2 cut(s) 63, 165
BseNI ACTGG 1 cut(s) 34
BseXI GCAGC 2 cut(s) 91, 194
BshFI GGCC 2 cut(s) 14, 364
BsiHKAI GWGCWC 1 cut(s) 228
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 1 cut(s) 207
BslFI GGGAC 1 cut(s) 147
BsmAI GTCTC 2 cut(s) 73, 315
BsmBI CGTCTC 1 cut(s) 315
BsmFI GGGAC 1 cut(s) 147
BsnI GGCC 2 cut(s) 14, 364
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 228
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 1 cut(s) 339
BspANI GGCC 2 cut(s) 14, 364
BspCNI CTCAG 2 cut(s) 64, 164
BspMAI CTGCAG 1 cut(s) 184
BsrDI GCAATG 2 cut(s) 63, 114
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 1 cut(s) 34
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 72, 151
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 4 cut(s) 118, 133, 331, 344
BstKTI GATC 1 cut(s) 342
BstMAI GTCTC 2 cut(s) 73, 315
BstMBI GATC 1 cut(s) 339
BstMWI GCNNNNNNNGC 2 cut(s) 44, 53
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 2 cut(s) 180, 291
BstV1I GCAGC 2 cut(s) 91, 194
BsuRI GGCC 2 cut(s) 14, 364
BtgI CCRYGG 1 cut(s) 379
BtrI CACGTC 1 cut(s) 139
BtsCI GGATG 4 cut(s) 118, 133, 331, 344
CaiI CAGNNNCTG 1 cut(s) 29
Cfr13I GGNCC 1 cut(s) 362
CseI GACGC 1 cut(s) 332
Csp6I GTAC 1 cut(s) 61
CviAII CATG 2 cut(s) 34, 44
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 2 cut(s) 72, 151
DpnI GATC 1 cut(s) 341
DpnII GATC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 108
Eco88I CYCGRG 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 226
EcoRI GAATTC 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 228
Esp3I CGTCTC 1 cut(s) 315
FaeI CATG 2 cut(s) 37, 47
FaiI YATR 5 cut(s) 35, 45, 96, 266, 278
FaqI GGGAC 1 cut(s) 147
FatI CATG 2 cut(s) 33, 43
Fnu4HI GCNGC 2 cut(s) 105, 183
FokI GGATG 4 cut(s) 125, 140, 318, 331
FriOI GRGCYC 1 cut(s) 228
Fsp4HI GCNGC 2 cut(s) 105, 183
GluI GCNGC 2 cut(s) 105, 183
HaeIII GGCC 2 cut(s) 14, 364
HapII CCGG 1 cut(s) 207
HgaI GACGC 1 cut(s) 332
Hin1II CATG 2 cut(s) 37, 47
HinfI GANTC 1 cut(s) 235
HpaII CCGG 1 cut(s) 207
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 2 cut(s) 335, 357
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 1 cut(s) 138
HpyCH4V TGCA 2 cut(s) 107, 182
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 53
HpyF3I CTNAG 2 cut(s) 72, 151
HpySE526I ACGT 1 cut(s) 138
Hsp92II CATG 2 cut(s) 37, 47
Kzo9I GATC 1 cut(s) 339
LpnPI CCDG 4 cut(s) 15, 171, 180, 220
Lsp1109I GCAGC 2 cut(s) 91, 194
LweI GCATC 1 cut(s) 353
MaeII ACGT 1 cut(s) 138
MalI GATC 1 cut(s) 341
MboI GATC 1 cut(s) 339
MhlI GDGCHC 1 cut(s) 228
MluCI AATT 2 cut(s) 168, 384
MmeI TCCRAC 1 cut(s) 226
MnlI CCTC 4 cut(s) 4, 118, 158, 353
MseI TTAA 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 185
MspI CCGG 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 44, 53
NciI CCSGG 1 cut(s) 207
NdeII GATC 1 cut(s) 339
NlaIII CATG 2 cut(s) 37, 47
PfeI GAWTC 1 cut(s) 235
PkrI GCNGC 2 cut(s) 106, 184
Psp124BI GAGCTC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 362
PstI CTGCAG 1 cut(s) 184
PstNI CAGNNNCTG 1 cut(s) 29
PvuII CAGCTG 1 cut(s) 185
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SacI GAGCTC 1 cut(s) 228
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 2 cut(s) 105, 183
Sau3AI GATC 1 cut(s) 339
Sau96I GGNCC 1 cut(s) 362
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 228
SetI ASST 6 cut(s) 28, 94, 141, 187, 228, 292
SfaNI GCATC 1 cut(s) 353
SfcI CTRYAG 2 cut(s) 180, 291
Sse9I AATT 2 cut(s) 168, 384
SspI AATATT 1 cut(s) 270
SstI GAGCTC 1 cut(s) 228
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 1 cut(s) 141
TasI AATT 2 cut(s) 168, 384
TatI WGTACW 1 cut(s) 60
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TseI GCWGC 2 cut(s) 104, 182
TspDTI ATGAA 2 cut(s) 320, 342
TspGWI ACGGA 1 cut(s) 247
XapI RAATTY 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.