MD04G1127700.v1.1

Hydrolyzes acetyl esters in homogalacturonan regions of pectin. In type I primary cell wall, galacturonic acid residues of pectin can be acetylated at the O-2 and O-3 positions. Decreasing the degree of acetylation of pectin gels in vitro alters their physical properties

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0028679
Physical Location & Seq
Forward (+)
21347777 .. 21349111
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1127700.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGTGGGGCCAATGGCGAATCCCAGGCTTCGCAGCCTTCTATGGTGGCGAAGATGGGCCAAAAAGGACTGGGCAATCGCCACCGTCGGATTTACCGTTATCGCCTTCGCTCTCACTCTCCTCTCCAACTCTTGGCGCGACGAAGCAGACTCCGAAATCTCCCGCCCGTTTCTTCCCAACGCCGCCGCCTTCGATTTCGTCGACTTGACCTTGCTGCGCAACGCCAAAGATAGAGGGGCCATTTGTTTGGATGGGACTGCCCCTGGCTACCATTTCAGGAAGGGATTCGGATCCGGCGCCAACAATTGGCTGCTTCACATTGAGTTCAAATTCTATCCCTGTGCCTTTTTTACAGGTCTAATACAAATTGGTGGAGGTTGGTGCAATTCGATTGAATCGTGTTCTTGGCGCAAAGGTACTATACTAGGGTCTTCTAATTACATGGATCGTCGAGTTCCCTTTTCTGGGATTTTGAGCTCTCATCCATCACAAAATCCTGGAACAAAGTCAAAATCAGATAGTGTGATGGCGCATCCTTCGCTGGCCACCCGGAAAATGAGCCTAAGGTGCATTAAAGGATCAGGACCTTTTTTTAGGGGCCAGCTTATCTGGGAAGCAGTTATGGATGAACTCTTGTCAATTGGCTTGTCAAAAGTGAAACCGGTATCTCGTGGTTACTTCGGTTAA

Protein Analysis

229

Amino Acids

25.33

Weight (kDa)

9.84

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAE PF03283 65 - 220 9.1e-33 Pectinacetylesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028679)

Species Orthologous Gene IDs
malus_domestica MD04G1127700.v1.1
pyrus_communis pycom126g00070

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 296
AccB7I CCANNNNNTGG 2 cut(s) 132, 306
AccI GTMKAC 1 cut(s) 201
AccII CGCG 1 cut(s) 138
AciI CCGC 3 cut(s) 163, 183, 186
AclWI GGATC 4 cut(s) 285, 298, 453, 586
AcoI YGGCCR 1 cut(s) 543
AcsI RAATTY 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 297
AfaI GTAC 1 cut(s) 418
AfiI CCNNNNNNNGG 4 cut(s) 132, 306, 464, 465
AgeI ACCGGT 1 cut(s) 661
AgsI TTSAA 2 cut(s) 328, 395
AjnI CCWGG 3 cut(s) 23, 262, 496
AluBI AGCT 2 cut(s) 477, 604
AluI AGCT 2 cut(s) 477, 604
Alw21I GWGCWC 1 cut(s) 479
AlwI GGATC 4 cut(s) 285, 298, 453, 586
AoxI GGCC 5 cut(s) 8, 57, 237, 543, 598
ApeKI GCWGC 3 cut(s) 33, 214, 310
ApoI RAATTY 1 cut(s) 329
AsiGI ACCGGT 1 cut(s) 661
Asp700I GAANNNNTTC 1 cut(s) 284
AspLEI GCGC 5 cut(s) 138, 219, 299, 411, 532
AspS9I GGNCC 5 cut(s) 8, 57, 237, 584, 598
AsuC2I CCSGG 1 cut(s) 550
AvaII GGWCC 1 cut(s) 584
AxyI CCTNAGG 1 cut(s) 563
BalI TGGCCA 1 cut(s) 545
BamHI GGATCC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 296
BanII GRGCYC 1 cut(s) 479
BauI CACGAG 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 45, 201, 297
BccI CCATC 4 cut(s) 48, 245, 493, 520
BciT130I CCWGG 3 cut(s) 25, 264, 498
BcnI CCSGG 1 cut(s) 550
BfaI CTAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 5 cut(s) 34, 183, 186, 215, 311
BlsI GCNGC 5 cut(s) 35, 184, 187, 216, 312
Bme1390I CCNGG 4 cut(s) 25, 264, 498, 550
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 5 cut(s) 8, 57, 237, 584, 598
BmiI GGNNCC 5 cut(s) 9, 238, 292, 298, 599
BmrFI CCNGG 4 cut(s) 25, 264, 498, 550
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 550
BsaBI GATNNNNATC 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 23, 262
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 4 cut(s) 132, 306, 464, 465
Bse118I RCCGGY 1 cut(s) 661
Bse1I ACTGG 1 cut(s) 74
Bse21I CCTNAGG 1 cut(s) 563
Bse8I GATNNNNATC 1 cut(s) 289
BseBI CCWGG 3 cut(s) 25, 264, 498
BseDI CCNNGG 2 cut(s) 23, 262
BseGI GGATG 4 cut(s) 256, 481, 532, 631
BseJI GATNNNNATC 1 cut(s) 289
BseLI CCNNNNNNNGG 4 cut(s) 132, 306, 464, 465
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 1 cut(s) 110
BseXI GCAGC 3 cut(s) 45, 201, 297
Bsh1236I CGCG 1 cut(s) 138
BshFI GGCC 5 cut(s) 10, 59, 239, 545, 600
BshNI GGYRCC 1 cut(s) 296
BshTI ACCGGT 1 cut(s) 661
BsiHKAI GWGCWC 1 cut(s) 479
BsiSI CCGG 3 cut(s) 294, 550, 662
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 4 cut(s) 132, 306, 464, 465
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 5 cut(s) 10, 59, 239, 545, 600
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 3 cut(s) 290, 445, 578
BspACI CCGC 3 cut(s) 163, 183, 186
BspANI GGCC 5 cut(s) 10, 59, 239, 545, 600
BspFNI CGCG 1 cut(s) 138
BspLI GGNNCC 5 cut(s) 9, 238, 292, 298, 599
BspPI GGATC 4 cut(s) 285, 298, 453, 586
BspT107I GGYRCC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 661
BsrI ACTGG 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 661
BssECI CCNNGG 2 cut(s) 23, 262
BssMI GATC 3 cut(s) 290, 445, 578
BssNI GRCGYC 1 cut(s) 297
BssSI CACGAG 1 cut(s) 669
Bst2BI CACGAG 1 cut(s) 669
Bst2UI CCWGG 3 cut(s) 25, 264, 498
Bst4CI ACNGT 2 cut(s) 85, 97
BstACI GRCGYC 1 cut(s) 297
BstC8I GCNNGC 2 cut(s) 543, 602
BstDEI CTNAG 1 cut(s) 563
BstF5I GGATG 4 cut(s) 256, 481, 532, 631
BstFNI CGCG 1 cut(s) 138
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 5 cut(s) 138, 219, 299, 411, 532
BstKTI GATC 3 cut(s) 293, 448, 581
BstMBI GATC 3 cut(s) 290, 445, 578
BstMWI GCNNNNNNNGC 2 cut(s) 538, 567
BstNI CCWGG 3 cut(s) 25, 264, 498
BstSCI CCNGG 4 cut(s) 23, 262, 496, 548
BstUI CGCG 1 cut(s) 138
BstV1I GCAGC 3 cut(s) 45, 201, 297
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 290
BstXI CCANNNNNNTGG 1 cut(s) 247
BstYI RGATCY 1 cut(s) 290
Bsu36I CCTNAGG 1 cut(s) 563
BsuRI GGCC 5 cut(s) 10, 59, 239, 545, 600
BtsCI GGATG 4 cut(s) 256, 481, 532, 631
Cac8I GCNNGC 2 cut(s) 543, 602
CfoI GCGC 5 cut(s) 138, 219, 299, 411, 532
Cfr10I RCCGGY 1 cut(s) 661
Cfr13I GGNCC 5 cut(s) 8, 57, 237, 584, 598
Csp6I GTAC 1 cut(s) 417
CspAI ACCGGT 1 cut(s) 661
CviAII CATG 1 cut(s) 442
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 1 cut(s) 563
DinI GGCGCC 1 cut(s) 298
DpnI GATC 3 cut(s) 292, 447, 580
DpnII GATC 3 cut(s) 290, 445, 578
EaeI YGGCCR 1 cut(s) 543
Ecl136II GAGCTC 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 479
Eco47I GGWCC 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 477
Eco81I CCTNAGG 1 cut(s) 563
EcoICRI GAGCTC 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 584
EcoRII CCWGG 3 cut(s) 23, 262, 496
EcoT38I GRGCYC 1 cut(s) 479
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
FaeI CATG 1 cut(s) 445
FaiI YATR 4 cut(s) 43, 422, 443, 623
FaqI GGGAC 1 cut(s) 268
FatI CATG 1 cut(s) 441
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 5 cut(s) 34, 183, 186, 215, 311
FokI GGATG 4 cut(s) 263, 468, 519, 638
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 5 cut(s) 34, 183, 186, 215, 311
FspBI CTAG 1 cut(s) 425
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 5 cut(s) 137, 218, 298, 410, 531
GluI GCNGC 5 cut(s) 34, 183, 186, 215, 311
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 5 cut(s) 10, 59, 239, 545, 600
HapII CCGG 3 cut(s) 294, 550, 662
HhaI GCGC 5 cut(s) 138, 219, 299, 411, 532
Hin1I GRCGYC 1 cut(s) 297
Hin1II CATG 1 cut(s) 445
Hin6I GCGC 5 cut(s) 136, 217, 297, 409, 530
HinP1I GCGC 5 cut(s) 136, 217, 297, 409, 530
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 4 cut(s) 19, 149, 285, 395
HpaII CCGG 3 cut(s) 294, 550, 662
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 4 cut(s) 89, 154, 290, 517
Hpy188III TCNNGA 2 cut(s) 277, 582
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 4 cut(s) 89, 143, 203, 453
HpyAV CCTTC 5 cut(s) 47, 115, 199, 274, 546
HpyCH4III ACNGT 2 cut(s) 85, 97
HpyCH4V TGCA 2 cut(s) 384, 570
HpyF10VI GCNNNNNNNGC 2 cut(s) 538, 567
HpyF3I CTNAG 1 cut(s) 563
Hsp92I GRCGYC 1 cut(s) 297
Hsp92II CATG 1 cut(s) 445
HspAI GCGC 5 cut(s) 136, 217, 297, 409, 530
KasI GGCGCC 1 cut(s) 296
Kzo9I GATC 3 cut(s) 290, 445, 578
Lsp1109I GCAGC 3 cut(s) 45, 201, 297
LweI GCATC 1 cut(s) 541
MaeI CTAG 1 cut(s) 425
MaeIII GTNAC 1 cut(s) 674
MalI GATC 3 cut(s) 292, 447, 580
MboI GATC 3 cut(s) 290, 445, 578
MboII GAAGA 3 cut(s) 63, 164, 423
MfeI CAATTG 2 cut(s) 304, 639
MflI RGATCY 1 cut(s) 290
MhlI GDGCHC 1 cut(s) 479
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 6 cut(s) 304, 329, 366, 385, 436, 639
MluNI TGGCCA 1 cut(s) 545
Mly113I GGCGCC 1 cut(s) 297
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 2 cut(s) 67, 150
MnlI CCTC 3 cut(s) 131, 227, 368
Mox20I TGGCCA 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 284
MscI TGGCCA 1 cut(s) 545
MseI TTAA 2 cut(s) 573, 685
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 3 cut(s) 294, 550, 662
MspR9I CCNGG 4 cut(s) 25, 264, 498, 550
MunI CAATTG 2 cut(s) 304, 639
MvaI CCWGG 3 cut(s) 25, 264, 498
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 2 cut(s) 538, 567
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 1 cut(s) 550
NdeII GATC 3 cut(s) 290, 445, 578
NlaIII CATG 1 cut(s) 445
NlaIV GGNNCC 5 cut(s) 9, 238, 292, 298, 599
NsbI TGCGCA 1 cut(s) 218
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 198, 395
PdmI GAANNNNTTC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 19, 285, 395
PflMI CCANNNNNTGG 2 cut(s) 132, 306
PfoI TCCNGGA 1 cut(s) 496
PinAI ACCGGT 1 cut(s) 661
PkrI GCNGC 5 cut(s) 35, 184, 187, 216, 312
PleI GAGTC 1 cut(s) 143
PluTI GGCGCC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 479
Psp5II RGGWCCY 1 cut(s) 584
Psp6I CCWGG 3 cut(s) 23, 262, 496
PspGI CCWGG 3 cut(s) 23, 262, 496
PspN4I GGNNCC 5 cut(s) 9, 238, 292, 298, 599
PspPI GGNCC 5 cut(s) 8, 57, 237, 584, 598
PspPPI RGGWCCY 1 cut(s) 584
PsuI RGATCY 1 cut(s) 290
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
SacI GAGCTC 1 cut(s) 479
SalI GTCGAC 1 cut(s) 200
SaqAI TTAA 2 cut(s) 573, 685
SatI GCNGC 5 cut(s) 34, 183, 186, 215, 311
Sau3AI GATC 3 cut(s) 290, 445, 578
Sau96I GGNCC 5 cut(s) 8, 57, 237, 584, 598
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 4 cut(s) 25, 264, 498, 550
SduI GDGCHC 1 cut(s) 479
SetI ASST 8 cut(s) 212, 358, 379, 418, 479, 569, 589, 606
SfaNI GCATC 1 cut(s) 541
SfoI GGCGCC 1 cut(s) 298
SinI GGWCC 1 cut(s) 584
Sse9I AATT 6 cut(s) 304, 329, 366, 385, 436, 639
SsiI CCGC 3 cut(s) 163, 183, 186
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 425
SstI GAGCTC 1 cut(s) 479
StyD4I CCNGG 4 cut(s) 23, 262, 496, 548
TaaI ACNGT 2 cut(s) 85, 97
TaqI TCGA 4 cut(s) 192, 201, 389, 451
TasI AATT 6 cut(s) 304, 329, 366, 385, 436, 639
TauI GCSGC 2 cut(s) 185, 188
TfiI GAWTC 3 cut(s) 19, 285, 395
Tru1I TTAA 2 cut(s) 573, 685
Tru9I TTAA 2 cut(s) 573, 685
TseI GCWGC 3 cut(s) 33, 214, 310
TspDTI ATGAA 1 cut(s) 642
Van91I CCANNNNNTGG 2 cut(s) 132, 306
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 1 cut(s) 329
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 284
XspI CTAG 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.