MD05G1078400.v1.1

Presequence protease 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0028836
Physical Location & Seq
Forward (+)
16604549 .. 16605450
902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1078400.v1.1.491

Sequence Viewer

Length: 606 bp
ATGTTGCTGGAATATGGCTATGGAGATATTTTTATTGAGGTTAATTTTCATCTCCTTCAACCTCTATTGAGTATATTGGTGATGAATGTTTCTCGCGATGGCATTTCAAAGGTAGAGCTAGTAGTCACGAGTACACTTATACTATTTGCGGAGAAAGGCTTTGATACAGATGCTGTAGAAGCATCCATGAATACAATGGAGTTCATTTGCGGGGAACATAATAAGAAAATACTTCCTGAAGGCTTGTTCTTAATGCTCCAGTCCATTGATAAATGGATAAATGGTATGGATCCCTTTGATCCACTGAAGCATGGCAATTTTTTAAAGGAACTGAATGCCAGAATAAAAAAGGAAGGCTCTAAAGCTCCTGATCCACAAAAGTTTTCTGGTGATAAAAAAGATGAGGAAGAAATCTTGGGGAAAGTTAAAGCCGGTATGACAGAAAATGATCTCGCTGAACTGGTACATGCTACCCGAGAGCTGCGACTGAAGCAGATAAATCCTGACCCACCAGAAGCTTTGAGAAGTGTTCCAAGCCTCTCTCTGCAAGACATTCCAAAACCTATTCATGTTCCCCCAAGGGTCTTAATCACCCTAAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M16C_assoc Peptidase_M16 Peptidase_M16_C

Protein Analysis

202

Amino Acids

22.57

Weight (kDa)

5.43

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
M16C_assoc PF08367 123 - 189 8.5e-11 Peptidase M16C associated
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028836)

Species Orthologous Gene IDs
malus_domestica MD05G1078400.v1.1
pyrus_communis pycom05g07180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 96
AciI CCGC 2 cut(s) 149, 210
AclWI GGATC 4 cut(s) 284, 293, 297, 365
AcuI CTGAAG 3 cut(s) 258, 326, 509
AfaI GTAC 2 cut(s) 133, 465
AgsI TTSAA 2 cut(s) 59, 108
AluBI AGCT 4 cut(s) 118, 365, 481, 518
AluI AGCT 4 cut(s) 118, 365, 481, 518
AlwI GGATC 4 cut(s) 284, 293, 297, 365
AlwNI CAGNNNCTG 1 cut(s) 173
Ama87I CYCGRG 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 481
AsuHPI GGTGA 3 cut(s) 91, 401, 583
AvaI CYCGRG 1 cut(s) 474
BamHI GGATCC 1 cut(s) 289
BauI CACGAG 1 cut(s) 127
BbvI GCAGC 1 cut(s) 468
BccI CCATC 1 cut(s) 92
BfaI CTAG 1 cut(s) 119
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 1 cut(s) 482
BlsI GCNGC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 291
BmsI GCATC 2 cut(s) 160, 191
BpmI CTGGAG 1 cut(s) 242
BsaJI CCNNGG 1 cut(s) 578
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 259, 465
BseDI CCNNGG 1 cut(s) 578
BseGI GGATG 1 cut(s) 182
BseNI ACTGG 2 cut(s) 259, 465
BseXI GCAGC 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 1 cut(s) 432
BsmI GAATGC 1 cut(s) 340
BsoBI CYCGRG 1 cut(s) 474
Bsp143I GATC 4 cut(s) 289, 298, 370, 448
Bsp68I TCGCGA 1 cut(s) 96
BspACI CCGC 2 cut(s) 149, 210
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 4 cut(s) 284, 293, 297, 365
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 259, 465
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 1 cut(s) 578
BssMI GATC 4 cut(s) 289, 298, 370, 448
BssSI CACGAG 1 cut(s) 127
BssT1I CCWWGG 1 cut(s) 578
Bst2BI CACGAG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 96
BstKTI GATC 4 cut(s) 292, 301, 373, 451
BstMBI GATC 4 cut(s) 289, 298, 370, 448
BstMWI GCNNNNNNNGC 2 cut(s) 179, 490
BstNSI RCATGY 1 cut(s) 470
BstSFI CTRYAG 1 cut(s) 174
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 1 cut(s) 182
BtsIMutI CAGTG 1 cut(s) 302
BtuMI TCGCGA 1 cut(s) 96
CaiI CAGNNNCTG 1 cut(s) 173
Cfr10I RCCGGY 1 cut(s) 431
Csp6I GTAC 2 cut(s) 132, 464
CviAII CATG 4 cut(s) 187, 311, 467, 569
CviQI GTAC 2 cut(s) 132, 464
DpnI GATC 4 cut(s) 291, 300, 372, 450
DpnII GATC 4 cut(s) 289, 298, 370, 448
DraI TTTAAA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 578
Eco57I CTGAAG 3 cut(s) 258, 326, 509
Eco88I CYCGRG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 578
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 4 cut(s) 190, 314, 470, 572
FatI CATG 4 cut(s) 186, 310, 466, 568
FauI CCCGC 1 cut(s) 203
Fnu4HI GCNGC 1 cut(s) 482
FokI GGATG 1 cut(s) 169
Fsp4HI GCNGC 1 cut(s) 482
FspBI CTAG 1 cut(s) 119
GluI GCNGC 1 cut(s) 482
GsuI CTGGAG 1 cut(s) 242
HapII CCGG 1 cut(s) 432
Hin1II CATG 4 cut(s) 190, 314, 470, 572
HindIII AAGCTT 1 cut(s) 516
HpaII CCGG 1 cut(s) 432
HphI GGTGA 3 cut(s) 91, 401, 583
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 1 cut(s) 605
Hpy188III TCNNGA 5 cut(s) 95, 127, 236, 368, 503
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 3 cut(s) 65, 233, 347
HpyCH4V TGCA 1 cut(s) 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 490
Hsp92II CATG 4 cut(s) 190, 314, 470, 572
Kzo9I GATC 4 cut(s) 289, 298, 370, 448
LmnI GCTCC 2 cut(s) 261, 370
LpnPI CCDG 9 cut(s) 249, 272, 352, 372, 381, 445, 446, 516, 525
Lsp1109I GCAGC 1 cut(s) 468
LweI GCATC 2 cut(s) 160, 191
MaeI CTAG 1 cut(s) 119
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 291, 300, 372, 450
MboI GATC 4 cut(s) 289, 298, 370, 448
MboII GAAGA 1 cut(s) 419
MflI RGATCY 1 cut(s) 289
MluCI AATT 2 cut(s) 43, 316
MnlI CCTC 4 cut(s) 31, 72, 397, 548
MseI TTAA 5 cut(s) 42, 251, 323, 426, 587
MspI CCGG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 340
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 179, 490
NdeII GATC 4 cut(s) 289, 298, 370, 448
NlaIII CATG 4 cut(s) 190, 314, 470, 572
NlaIV GGNNCC 1 cut(s) 291
NmuCI GTSAC 1 cut(s) 124
NruI TCGCGA 1 cut(s) 96
NspI RCATGY 1 cut(s) 470
PctI GAATGC 1 cut(s) 340
PkrI GCNGC 1 cut(s) 483
PspN4I GGNNCC 1 cut(s) 291
PstNI CAGNNNCTG 1 cut(s) 173
PsuI RGATCY 1 cut(s) 289
RruI TCGCGA 1 cut(s) 96
RsaI GTAC 2 cut(s) 133, 465
RsaNI GTAC 2 cut(s) 132, 464
SaqAI TTAA 5 cut(s) 42, 251, 323, 426, 587
SatI GCNGC 1 cut(s) 482
Sau3AI GATC 4 cut(s) 289, 298, 370, 448
SetI ASST 8 cut(s) 42, 64, 114, 120, 367, 483, 520, 565
SfaNI GCATC 2 cut(s) 160, 191
SfcI CTRYAG 1 cut(s) 174
Sse9I AATT 2 cut(s) 43, 316
SsiI CCGC 2 cut(s) 149, 210
SspMI CTAG 1 cut(s) 119
StyI CCWWGG 1 cut(s) 578
TasI AATT 2 cut(s) 43, 316
TatI WGTACW 1 cut(s) 131
Tru1I TTAA 5 cut(s) 42, 251, 323, 426, 587
Tru9I TTAA 5 cut(s) 42, 251, 323, 426, 587
TscAI CASTG 1 cut(s) 309
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 1 cut(s) 481
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 5 cut(s) 38, 98, 193, 203, 557
TspRI CASTG 1 cut(s) 309
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XspI CTAG 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.